Rw5G017740

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008571
Physical Location & Seq
Forward (+)
22652275 .. 22656272
3998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017740.1

Sequence Viewer

Length: 1407 bp
ATGCCGAAGAAGAGAGGGAGAGAGAGGCATCACAACCCGGAGCCATTTCTGGCCGACGACGATGGCGCCTCTGTGGCCTCCAAGAAGCGGACCAAGTCCAAGCAGCACCAGACCGACGAGACTCTGATTTCGTCGGGCATGAGCGCCAAGATTCTCAAGCAGGCTCAGCTTCAGCAGAGGGAGGTCGAGGACGACGAGAATCGAACCCAAAACCCCAACGCCTTATCGGACATCACCGAAGAGCCGCACGGGGTTGAATCTGATGACGACGACCCCGATGAGTTCGGTGGGTTTCTTGACAATCGCGGCGAGGATGAAGCGTTTAAAGTGACGAACAGGATGACAAAGGAGATTGGGAAGGAGTATGAGAAAGTATTGGGTAAGGCGGCGGTGGAAGAGGATGAGAAATTACTGGATGCATTTCTTTCGAAGGATGCAGGGCGGCAGCTTACTCTTGCAGACCTGATTGTTGCGAGGATTAAGGAGAATGATTCTAATATCACTCCATCAAATGCCAATTCAGATATGAGACCTTTGCCTAAATTGGATGCAGACACCATAGAAATCTTCAAGGGGGTTGGCCAGTTTCTTAAAAAGTACACAGCTGGGAAGGTTCCCAAACCCTTTAAACACATCCCTTCAATGAATCAATGGGAAGATGTCCTATATTTAACTGAGCCTGAGAATTGGTCTCCCAATGCGATGTATCAAGCTACAAGAATTTTTGCTTCCAATTTGACTGCGAAGAAGGTAGAACGTTTCTACAAGCTTGTCTTGCTTCCACGTGTGAGAGAAGACATTCGGAAGAACAAGCGCTTGCATTTTGCCCTTTATCAGGCTTTGAAAAAGTCTCTTTACAAGCCTGCTGCCTTTTTCAAAGGAATATTGCTACCTTTGTGCGAGTCAGGGACGTGCAATCTAAGAGAAGCTGTCATAGTTGGGAGCGTCTTACAAAAGGTTTCCATTCCGCCACTTCATTCAAGTGTTGCAGTAATGAAGCTAGCAGACATGGAATACTGTGGTACAACAAGTTATTTTATAAAGCTTCTTTTGGAGAAAAAGTATGCTTTGCCATATCGTGTAATTGATGCTCTTGTTGGACATTTTATGAGATTTATGAATGAAACAAGGGTAATGCCAGTGATATGGCACCAATCACTTCTTGCATTTGTGGAAAGGTACAAAAATGATATACTGAATGAGGATAAAGATAATCTTAGAGTTCTTCTTCAAACTCAAAAGCACGATCTGGTAACTCGTGAAATAAGAAAGGAGCTAGATAATAGCCGCAGTCGTGGTGAGAAGGAGGATGACCTTATGTCAATTGCTTCTCCAGATTCTGTGATGATTAAGCCTGTTGAAGAAGATATGTTTGATATTCCAGAGGTGCCTATGGAGGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

468

Amino Acids

53.52

Weight (kDa)

5.84

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 148 - 435 1.9e-130 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1040
AccB1I GGYRCC 3 cut(s) 65, 1149, 1387
AccII CGCG 1 cut(s) 306
AciI CCGC 8 cut(s) 88, 245, 306, 386, 389, 442, 968, 1288
AclI AACGTT 1 cut(s) 757
AcoI YGGCCR 2 cut(s) 51, 580
AcsI RAATTY 1 cut(s) 720
AcuI CTGAAG 1 cut(s) 155
AcvI CACGTG 1 cut(s) 785
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 3 cut(s) 599, 1024, 1181
AfeI AGCGCT 1 cut(s) 815
AfiI CCNNNNNNNGG 2 cut(s) 87, 835
AflIII ACRYGT 1 cut(s) 784
AgsI TTSAA 8 cut(s) 257, 571, 642, 844, 877, 981, 1232, 1361
AhdI GACNNNNNGTC 1 cut(s) 1318
AjiI CACGTC 1 cut(s) 912
AluBI AGCT 9 cut(s) 169, 448, 605, 713, 769, 929, 1000, 1045, 1276
AluI AGCT 9 cut(s) 169, 448, 605, 713, 769, 929, 1000, 1045, 1276
Alw26I GTCTC 4 cut(s) 113, 523, 696, 855
AlwNI CAGNNNCTG 1 cut(s) 1340
Aor51HI AGCGCT 1 cut(s) 815
AoxI GGCC 3 cut(s) 51, 75, 580
ApeKI GCWGC 3 cut(s) 103, 445, 866
ApoI RAATTY 1 cut(s) 720
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
Asp700I GAANNNNTTC 1 cut(s) 798
AspLEI GCGC 3 cut(s) 68, 146, 816
AspS9I GGNCC 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 226, 1310
AsuII TTCGAA 1 cut(s) 428
AsuNHI GCTAGC 1 cut(s) 1000
AvaII GGWCC 1 cut(s) 90
BalI TGGCCA 1 cut(s) 582
BanI GGYRCC 3 cut(s) 65, 1149, 1387
BauI CACGAG 1 cut(s) 1257
BbrPI CACGTG 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 801
BbvI GCAGC 3 cut(s) 115, 457, 853
BccI CCATC 2 cut(s) 56, 514
BcgI CGANNNNNNTGC 2 cut(s) 408, 442
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 4 cut(s) 113, 523, 696, 855
BfaI CTAG 2 cut(s) 1001, 1277
BfoI RGCGCY 3 cut(s) 69, 147, 817
BglI GCCNNNNNGGC 1 cut(s) 74
BisI GCNGC 8 cut(s) 104, 245, 307, 387, 443, 446, 867, 1288
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 8 cut(s) 105, 246, 308, 388, 444, 447, 868, 1289
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 1 cut(s) 90
BmeRI GACNNNNNGTC 1 cut(s) 1318
BmgBI CACGTC 1 cut(s) 912
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 5 cut(s) 42, 67, 615, 1151, 1389
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 5 cut(s) 37, 406, 424, 538, 1078
BmtI GCTAGC 1 cut(s) 1004
BpiI GAAGAC 1 cut(s) 801
BpmI CTGGAG 1 cut(s) 1317
Bpu1102I GCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 785
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 2 cut(s) 523, 696
BsaXI ACNNNNNCTCC 2 cut(s) 1046, 1076
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 835
Bse1I ACTGG 3 cut(s) 417, 583, 1139
BseGI GGATG 9 cut(s) 319, 345, 406, 421, 439, 553, 633, 1315, 1405
BseLI CCNNNNNNNGG 2 cut(s) 87, 835
BseMII CTCAG 3 cut(s) 179, 666, 672
BseNI ACTGG 3 cut(s) 417, 583, 1139
BseXI GCAGC 3 cut(s) 115, 457, 853
BseYI CCCAGC 1 cut(s) 605
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 3 cut(s) 53, 77, 582
BshNI GGYRCC 3 cut(s) 65, 1149, 1387
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 922
BslI CCNNNNNNNGG 2 cut(s) 87, 835
BsmAI GTCTC 4 cut(s) 113, 523, 696, 855
BsmFI GGGAC 1 cut(s) 922
BsnI GGCC 3 cut(s) 53, 77, 582
Bso31I GGTCTC 2 cut(s) 523, 696
Bsp119I TTCGAA 1 cut(s) 428
Bsp143I GATC 1 cut(s) 1246
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 8 cut(s) 88, 245, 306, 386, 389, 442, 968, 1288
BspANI GGCC 3 cut(s) 53, 77, 582
BspCNI CTCAG 3 cut(s) 178, 667, 673
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 5 cut(s) 42, 67, 615, 1151, 1389
BspOI GCTAGC 1 cut(s) 1004
BspQI GCTCTTC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 428
BspT107I GGYRCC 3 cut(s) 65, 1149, 1387
BspTNI GGTCTC 2 cut(s) 523, 696
BsrI ACTGG 3 cut(s) 417, 583, 1139
BssMI GATC 1 cut(s) 1246
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 1257
Bst2BI CACGAG 1 cut(s) 1257
Bst4CI ACNGT 1 cut(s) 1019
Bst6I CTCTTC 3 cut(s) 5, 234, 390
BstACI GRCGYC 1 cut(s) 66
BstBAI YACGTR 1 cut(s) 785
BstBI TTCGAA 1 cut(s) 428
BstC8I GCNNGC 4 cut(s) 162, 818, 864, 1002
BstDEI CTNAG 5 cut(s) 165, 675, 681, 920, 1217
BstENI CCTNNNNNAGG 1 cut(s) 833
BstF5I GGATG 9 cut(s) 319, 345, 406, 421, 439, 553, 633, 1315, 1405
BstFNI CGCG 1 cut(s) 306
BstH2I RGCGCY 3 cut(s) 69, 147, 817
BstHHI GCGC 3 cut(s) 68, 146, 816
BstKTI GATC 1 cut(s) 1249
BstMAI GTCTC 4 cut(s) 113, 523, 696, 855
BstMBI GATC 1 cut(s) 1246
BstMWI GCNNNNNNNGC 3 cut(s) 74, 166, 775
BstSCI CCNGG 1 cut(s) 36
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 3 cut(s) 115, 457, 853
BstV2I GAAGAC 1 cut(s) 801
BstXI CCANNNNNNTGG 1 cut(s) 1146
BsuRI GGCC 3 cut(s) 53, 77, 582
BtgZI GCGATG 1 cut(s) 716
BtrI CACGTC 1 cut(s) 912
BtsCI GGATG 9 cut(s) 319, 345, 406, 421, 439, 553, 633, 1315, 1405
BtsIMutI CAGTG 1 cut(s) 1146
Cac8I GCNNGC 4 cut(s) 162, 818, 864, 1002
CaiI CAGNNNCTG 1 cut(s) 1340
CfoI GCGC 3 cut(s) 68, 146, 816
Cfr13I GGNCC 1 cut(s) 90
CseI GACGC 1 cut(s) 934
Csp6I GTAC 3 cut(s) 598, 1023, 1180
CviAII CATG 2 cut(s) 139, 1009
CviQI GTAC 3 cut(s) 598, 1023, 1180
DdeI CTNAG 5 cut(s) 165, 675, 681, 920, 1217
DinI GGCGCC 1 cut(s) 67
DpnI GATC 1 cut(s) 1248
DpnII GATC 1 cut(s) 1246
DraI TTTAAA 2 cut(s) 325, 628
DriI GACNNNNNGTC 1 cut(s) 1318
EaeI YGGCCR 2 cut(s) 51, 580
Eam1104I CTCTTC 3 cut(s) 5, 234, 390
Eam1105I GACNNNNNGTC 1 cut(s) 1318
EarI CTCTTC 3 cut(s) 5, 234, 390
EciI GGCGGA 1 cut(s) 957
Eco31I GGTCTC 2 cut(s) 523, 696
Eco47I GGWCC 1 cut(s) 90
Eco47III AGCGCT 1 cut(s) 815
Eco57I CTGAAG 1 cut(s) 155
Eco72I CACGTG 1 cut(s) 785
EcoNI CCTNNNNNAGG 1 cut(s) 833
EcoT22I ATGCAT 1 cut(s) 421
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
FaeI CATG 2 cut(s) 142, 1012
FalI AAGNNNNNCTT 4 cut(s) 758, 790, 1200, 1232
FaqI GGGAC 1 cut(s) 922
FatI CATG 2 cut(s) 138, 1008
Fnu4HI GCNGC 8 cut(s) 104, 245, 307, 387, 443, 446, 867, 1288
FokI GGATG 8 cut(s) 326, 352, 413, 428, 446, 560, 620, 1322
Fsp4HI GCNGC 8 cut(s) 104, 245, 307, 387, 443, 446, 867, 1288
FspBI CTAG 2 cut(s) 1001, 1277
GlaI GCGC 3 cut(s) 67, 145, 815
GluI GCNGC 8 cut(s) 104, 245, 307, 387, 443, 446, 867, 1288
GsaI CCCAGC 1 cut(s) 609
GsuI CTGGAG 1 cut(s) 1317
HaeII RGCGCY 3 cut(s) 69, 147, 817
HaeIII GGCC 3 cut(s) 53, 77, 582
HapII CCGG 1 cut(s) 38
HgaI GACGC 1 cut(s) 934
HhaI GCGC 3 cut(s) 68, 146, 816
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 142, 1012
Hin6I GCGC 3 cut(s) 66, 144, 814
HinP1I GCGC 3 cut(s) 66, 144, 814
HindIII AAGCTT 2 cut(s) 767, 1043
HinfI GANTC 8 cut(s) 121, 151, 199, 257, 491, 646, 902, 1337
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 226, 1310
Hpy166II GTNNAC 1 cut(s) 600
Hpy188I TCNGA 5 cut(s) 126, 229, 262, 523, 804
Hpy188III TCNNGA 4 cut(s) 296, 1259, 1334, 1382
Hpy8I GTNNAC 1 cut(s) 600
Hpy99I CGWCG 6 cut(s) 59, 62, 119, 136, 197, 272
HpyAV CCTTC 6 cut(s) 352, 424, 604, 648, 742, 1297
HpyCH4III ACNGT 1 cut(s) 1019
HpyCH4IV ACGT 3 cut(s) 757, 784, 911
HpyCH4V TGCA 8 cut(s) 419, 437, 458, 551, 820, 915, 989, 1166
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 166, 775
HpyF3I CTNAG 5 cut(s) 165, 675, 681, 920, 1217
HpySE526I ACGT 3 cut(s) 757, 784, 911
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 142, 1012
HspAI GCGC 3 cut(s) 66, 144, 814
KasI GGCGCC 1 cut(s) 65
Kzo9I GATC 1 cut(s) 1246
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 3 cut(s) 40, 942, 1273
Lsp1109I GCAGC 3 cut(s) 115, 457, 853
LweI GCATC 5 cut(s) 37, 406, 424, 538, 1078
MaeI CTAG 2 cut(s) 1001, 1277
MaeII ACGT 3 cut(s) 757, 784, 911
MaeIII GTNAC 2 cut(s) 328, 1252
MalI GATC 1 cut(s) 1248
MboI GATC 1 cut(s) 1246
MfeI CAATTG 1 cut(s) 1323
MlsI TGGCCA 1 cut(s) 582
MluCI AATT 8 cut(s) 407, 517, 542, 685, 720, 733, 1083, 1323
MluNI TGGCCA 1 cut(s) 582
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 2 cut(s) 115, 911
MmeI TCCRAC 1 cut(s) 1078
Mox20I TGGCCA 1 cut(s) 582
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 798
MscI TGGCCA 1 cut(s) 582
MseI TTAA 6 cut(s) 324, 480, 591, 627, 671, 1350
MslI CAYNNNNRTG 1 cut(s) 981
Msp20I TGGCCA 1 cut(s) 582
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 38
MunI CAATTG 1 cut(s) 1323
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 74, 166, 775
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 38
NdeII GATC 1 cut(s) 1246
NheI GCTAGC 1 cut(s) 1000
NlaIII CATG 2 cut(s) 142, 1012
NlaIV GGNNCC 5 cut(s) 42, 67, 615, 1151, 1389
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 421
NspV TTCGAA 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 273
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 6 cut(s) 151, 199, 257, 491, 646, 1337
PflFI GACNNNGTC 1 cut(s) 94
PkrI GCNGC 8 cut(s) 105, 246, 308, 388, 444, 447, 868, 1289
PleI GAGTC 2 cut(s) 115, 910
PluTI GGCGCC 1 cut(s) 69
PmaCI CACGTG 1 cut(s) 785
PmlI CACGTG 1 cut(s) 785
PpsI GAGTC 2 cut(s) 115, 910
Ppu21I YACGTR 1 cut(s) 785
PsiI TTATAA 1 cut(s) 1040
Psp1406I AACGTT 1 cut(s) 757
PspCI CACGTG 1 cut(s) 785
PspFI CCCAGC 1 cut(s) 605
PspN4I GGNNCC 5 cut(s) 42, 67, 615, 1151, 1389
PspPI GGNCC 1 cut(s) 90
PstNI CAGNNNCTG 1 cut(s) 1340
PsyI GACNNNGTC 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 3 cut(s) 599, 1024, 1181
RsaNI GTAC 3 cut(s) 598, 1023, 1180
RseI CAYNNNNRTG 1 cut(s) 981
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 6 cut(s) 324, 480, 591, 627, 671, 1350
SatI GCNGC 8 cut(s) 104, 245, 307, 387, 443, 446, 867, 1288
Sau3AI GATC 1 cut(s) 1246
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 2 cut(s) 115, 911
ScrFI CCNGG 1 cut(s) 38
SfaNI GCATC 5 cut(s) 37, 406, 424, 538, 1078
SfoI GGCGCC 1 cut(s) 67
SfuI TTCGAA 1 cut(s) 428
SinI GGWCC 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 981
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 8 cut(s) 407, 517, 542, 685, 720, 733, 1083, 1323
SsiI CCGC 8 cut(s) 88, 245, 306, 386, 389, 442, 968, 1288
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 1 cut(s) 885
SspMI CTAG 2 cut(s) 1001, 1277
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 1019
TaiI ACGT 3 cut(s) 760, 787, 914
TaqI TCGA 3 cut(s) 186, 202, 428
TaqII GACCGA 1 cut(s) 128
TasI AATT 8 cut(s) 407, 517, 542, 685, 720, 733, 1083, 1323
TatI WGTACW 1 cut(s) 597
TauI GCSGC 5 cut(s) 247, 309, 389, 445, 1290
TfiI GAWTC 6 cut(s) 151, 199, 257, 491, 646, 1337
Tru1I TTAA 6 cut(s) 324, 480, 591, 627, 671, 1350
Tru9I TTAA 6 cut(s) 324, 480, 591, 627, 671, 1350
TscAI CASTG 1 cut(s) 1146
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 3 cut(s) 103, 445, 866
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 6 cut(s) 330, 659, 965, 1010, 1133, 1137
TspRI CASTG 1 cut(s) 1146
Tth111I GACNNNGTC 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 90
XagI CCTNNNNNAGG 1 cut(s) 833
XapI RAATTY 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 2 cut(s) 1001, 1277
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.