AT1G33290

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006008
Physical Location & Seq
Forward (+)
12074099 .. 12076289
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33290.1

Sequence Viewer

Length: 1140 bp
ATGGCCGGAGTTTGCGTCCCATGGGGCGGCGGAGCTTGTCGTCTTATTTCCCCAATTCGCCGGCGTTTTCCGGTTTCCTCCTCCGTCGCTTCTCTTGCTCTTCCAGACTCCTCCTCTATGATCGTCGACGACAATCTCGGCGCATTTCTCGAGATTCTTCCAAAAGATCTTCGTCACCGTCTCTTGAACGATTCAAGAAGAAACCAGCTCGTAGAGGTCATAATGGATTTAGGAAGACCACCTGAAGCACGTTATCTCGGAGAACCTGGAGGTCAATATCTTAGGAACAACGAGGTATCTATGGAAGAGTTAGAGGATGCTCAAGAGCTTGTAGGTGAGTTTGGAGCTGATAATAGAGCTGGAATAGAAGGTACATTGCACAGGATATCAGCTATTCGTAATCGGAAGGGATTCATTGTCGGTTTAACTTGTCGTGTTGGGAGAGCTGTGAGTGGTCACATTGACATGCTTTATGATCTTCTTCACTATGGCAAAAGCATCTTGTTCGTTGGACGGCCTGGTGTTGGTAAGACCACTGTTTTGCGAGAGATTGCTCGTGTCTTGTCTGATGAATTCCAGAAAAGAGTGGTGATAATTGATACAAGCAACGAAATAGGAGGAGATGGTGATATTCCACACTCAGCTATTGGAGGTTCGCGTAGAATGCAAGTACCTAAGCCATCTTTGCAGCATAAAGTGATGATTGAGGCAGTTGAGAATCATATGCCTCAAGTGATCATCGTTGATGAGATTGGCACAGAAGCTGAAGCACTTGCTTGTCGTTCCATTGCTGAAAGAGGAGTGATGCTAATTGGCACTGCTCATGGAGAGCAGCTACAGAACATCATAAAGAATCCTACTCTTTCAGACTTGATTGGTGGTATTGAGACTGTAACGCTTGGAGATGAAGAAGCGAGGGCGAGAAGGAGTCAGAAGAGTATTCTCGAGAGAAAAGCTCCACCGACTTTTTATTTTTTGATCGAGATGAGAGAAAGAGATTATTGGATTGCACATCAAACTGAAAAGAGTGTTGATATGTTGCTTCGCGGAAGAAATCCCATGGTTGAGGTGAGGAGAAGGGATGAAGAGTACAAGGTGGTGATAGAAAGATGGAAGGCATACGACGGGCAAGGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_30

Protein Analysis

379

Amino Acids

42.25

Weight (kDa)

6.35

Isoelectric Point (pI)

58.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spore_III_AA PF19568 131 - 293 5.9e-13 Sporulation stage III, protein AA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 126
AccII CGCG 2 cut(s) 658, 1047
AciI CCGC 3 cut(s) 27, 30, 1047
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 2 cut(s) 264, 786
AfaI GTAC 3 cut(s) 373, 672, 1091
AfiI CCNNNNNNNGG 2 cut(s) 26, 524
AgsI TTSAA 2 cut(s) 187, 195
AjnI CCWGG 2 cut(s) 265, 517
Alw26I GTCTC 2 cut(s) 185, 881
AlwNI CAGNNNCTG 1 cut(s) 764
Ama87I CYCGRG 2 cut(s) 149, 944
AoxI GGCC 2 cut(s) 3, 515
ApeKI GCWGC 2 cut(s) 688, 832
ApoI RAATTY 1 cut(s) 572
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
Asp700I GAANNNNTTC 1 cut(s) 410
AspLEI GCGC 1 cut(s) 143
AsuHPI GGTGA 6 cut(s) 167, 347, 601, 638, 1081, 1111
AvaI CYCGRG 2 cut(s) 149, 944
BauI CACGAG 1 cut(s) 555
BbsI GAAGAC 1 cut(s) 241
BbvI GCAGC 2 cut(s) 700, 844
BccI CCATC 3 cut(s) 617, 688, 1104
BceAI ACGGC 1 cut(s) 530
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 2 cut(s) 267, 519
BclI TGATCA 1 cut(s) 735
BcoDI GTCTC 2 cut(s) 185, 881
BfmI CTRYAG 1 cut(s) 836
BglII AGATCT 1 cut(s) 166
BisI GCNGC 3 cut(s) 28, 689, 833
BlsI GCNGC 3 cut(s) 29, 690, 834
Bme1390I CCNGG 2 cut(s) 267, 519
BmeT110I CYCGRG 2 cut(s) 149, 944
BmrFI CCNGG 2 cut(s) 267, 519
BmsI GCATC 3 cut(s) 307, 507, 795
BpiI GAAGAC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 940, 972
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 675
BpuEI CTTGAG 2 cut(s) 306, 714
BsaJI CCNNGG 2 cut(s) 20, 1059
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 524
Bse118I RCCGGY 1 cut(s) 60
Bse3DI GCAATG 2 cut(s) 374, 786
BseBI CCWGG 2 cut(s) 267, 519
BseDI CCNNGG 2 cut(s) 20, 1059
BseGI GGATG 2 cut(s) 322, 1087
BseLI CCNNNNNNNGG 2 cut(s) 26, 524
BseMI GCAATG 2 cut(s) 374, 786
BseMII CTCAG 1 cut(s) 654
BseRI GAGGAG 6 cut(s) 70, 100, 103, 633, 813, 1087
BseXI GCAGC 2 cut(s) 700, 844
Bsh1236I CGCG 2 cut(s) 658, 1047
BshFI GGCC 2 cut(s) 5, 517
BsiHKCI CYCGRG 2 cut(s) 149, 944
BsiSI CCGG 3 cut(s) 6, 61, 71
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 2 cut(s) 26, 524
BsmAI GTCTC 2 cut(s) 185, 881
BsmBI CGTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 2
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 2 cut(s) 5, 517
BsoBI CYCGRG 2 cut(s) 149, 944
Bsp143I GATC 5 cut(s) 120, 166, 475, 735, 978
Bsp19I CCATGG 2 cut(s) 20, 1059
BspACI CCGC 3 cut(s) 27, 30, 1047
BspANI GGCC 2 cut(s) 5, 517
BspCNI CTCAG 1 cut(s) 653
BspFNI CGCG 2 cut(s) 658, 1047
BspQI GCTCTTC 1 cut(s) 105
BsrDI GCAATG 2 cut(s) 374, 786
BsrFI RCCGGY 1 cut(s) 60
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 2 cut(s) 20, 1059
BssMI GATC 5 cut(s) 120, 166, 475, 735, 978
BssSI CACGAG 1 cut(s) 555
BssT1I CCWWGG 2 cut(s) 20, 1059
Bst2BI CACGAG 1 cut(s) 555
Bst2UI CCWGG 2 cut(s) 267, 519
Bst4CI ACNGT 3 cut(s) 179, 538, 892
Bst6I CTCTTC 4 cut(s) 105, 300, 929, 1080
BstC8I GCNNGC 1 cut(s) 62
BstDEI CTNAG 3 cut(s) 281, 640, 675
BstDSI CCRYGG 2 cut(s) 20, 1059
BstF5I GGATG 2 cut(s) 322, 1087
BstFNI CGCG 2 cut(s) 658, 1047
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 5 cut(s) 123, 169, 478, 738, 981
BstMAI GTCTC 2 cut(s) 185, 881
BstMBI GATC 5 cut(s) 120, 166, 475, 735, 978
BstMWI GCNNNNNNNGC 3 cut(s) 95, 664, 685
BstNI CCWGG 2 cut(s) 267, 519
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 265, 517
BstSFI CTRYAG 1 cut(s) 836
BstUI CGCG 2 cut(s) 658, 1047
BstV1I GCAGC 2 cut(s) 700, 844
BstV2I GAAGAC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 2 cut(s) 5, 517
BtgI CCRYGG 2 cut(s) 20, 1059
BtsCI GGATG 2 cut(s) 322, 1087
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 2 cut(s) 534, 816
Cac8I GCNNGC 1 cut(s) 62
CaiI CAGNNNCTG 1 cut(s) 764
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 60
CseI GACGC 1 cut(s) 4
Csp6I GTAC 3 cut(s) 372, 671, 1090
CviAII CATG 4 cut(s) 21, 466, 824, 1060
CviQI GTAC 3 cut(s) 372, 671, 1090
DdeI CTNAG 3 cut(s) 281, 640, 675
DpnI GATC 5 cut(s) 122, 168, 477, 737, 980
DpnII GATC 5 cut(s) 120, 166, 475, 735, 978
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 105, 300, 929, 1080
EarI CTCTTC 4 cut(s) 105, 300, 929, 1080
EciI GGCGGA 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 20, 1059
Eco32I GATATC 1 cut(s) 387
Eco57I CTGAAG 2 cut(s) 264, 786
Eco88I CYCGRG 2 cut(s) 149, 944
EcoRI GAATTC 1 cut(s) 572
EcoRII CCWGG 2 cut(s) 265, 517
EcoRV GATATC 1 cut(s) 387
EcoT14I CCWWGG 2 cut(s) 20, 1059
ErhI CCWWGG 2 cut(s) 20, 1059
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 4 cut(s) 24, 469, 827, 1063
FaqI GGGAC 1 cut(s) 2
FatI CATG 4 cut(s) 20, 465, 823, 1059
FauNDI CATATG 1 cut(s) 723
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 3 cut(s) 28, 689, 833
FokI GGATG 2 cut(s) 329, 1094
Fsp4HI GCNGC 3 cut(s) 28, 689, 833
GlaI GCGC 1 cut(s) 142
GluI GCNGC 3 cut(s) 28, 689, 833
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 2 cut(s) 5, 517
HapII CCGG 3 cut(s) 6, 61, 71
HgaI GACGC 1 cut(s) 4
HhaI GCGC 1 cut(s) 143
Hin1II CATG 4 cut(s) 24, 469, 827, 1063
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 7 cut(s) 107, 154, 191, 411, 718, 853, 928
HpaII CCGG 3 cut(s) 6, 61, 71
HphI GGTGA 6 cut(s) 167, 347, 601, 638, 1081, 1111
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 5 cut(s) 260, 405, 568, 868, 933
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 4 cut(s) 89, 128, 131, 1127
HpyAV CCTTC 5 cut(s) 362, 400, 918, 1071, 1108
HpyCH4III ACNGT 3 cut(s) 179, 538, 892
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 4 cut(s) 379, 667, 688, 1010
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 664, 685
HpyF3I CTNAG 3 cut(s) 281, 640, 675
HpySE526I ACGT 1 cut(s) 250
Hsp92II CATG 4 cut(s) 24, 469, 827, 1063
HspAI GCGC 1 cut(s) 141
KroI GCCGGC 1 cut(s) 60
KroNI GCCGGC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 735
Kzo9I GATC 5 cut(s) 120, 166, 475, 735, 978
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 3 cut(s) 32, 344, 961
Lsp1109I GCAGC 2 cut(s) 700, 844
LweI GCATC 3 cut(s) 307, 507, 795
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 3 cut(s) 173, 455, 892
MalI GATC 5 cut(s) 122, 168, 477, 737, 980
MboI GATC 5 cut(s) 120, 166, 475, 735, 978
MflI RGATCY 1 cut(s) 166
MluCI AATT 4 cut(s) 54, 572, 594, 810
MlyI GAGTC 2 cut(s) 101, 937
MmeI TCCRAC 1 cut(s) 490
MreI CGCCGGCG 1 cut(s) 60
MroNI GCCGGC 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 410
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 1 cut(s) 464
MspI CCGG 3 cut(s) 6, 61, 71
MspR9I CCNGG 2 cut(s) 267, 519
Mva1269I GAATGC 1 cut(s) 669
MvaI CCWGG 2 cut(s) 267, 519
MvnI CGCG 2 cut(s) 658, 1047
MwoI GCNNNNNNNGC 3 cut(s) 95, 664, 685
NaeI GCCGGC 1 cut(s) 62
NcoI CCATGG 2 cut(s) 20, 1059
NdeI CATATG 1 cut(s) 723
NdeII GATC 5 cut(s) 120, 166, 475, 735, 978
NgoMIV GCCGGC 1 cut(s) 60
NlaIII CATG 4 cut(s) 24, 469, 827, 1063
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 2 cut(s) 173, 455
NspI RCATGY 1 cut(s) 469
PaeR7I CTCGAG 2 cut(s) 149, 944
PciSI GCTCTTC 1 cut(s) 105
PctI GAATGC 1 cut(s) 669
PdiI GCCGGC 1 cut(s) 62
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 5 cut(s) 154, 191, 411, 718, 853
PkrI GCNGC 3 cut(s) 29, 690, 834
PleI GAGTC 2 cut(s) 101, 936
PpsI GAGTC 2 cut(s) 101, 936
Psp6I CCWGG 2 cut(s) 265, 517
PspGI CCWGG 2 cut(s) 265, 517
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 1 cut(s) 166
RsaI GTAC 3 cut(s) 373, 672, 1091
RsaNI GTAC 3 cut(s) 372, 671, 1090
RseI CAYNNNNRTG 1 cut(s) 464
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 3 cut(s) 28, 689, 833
Sau3AI GATC 5 cut(s) 120, 166, 475, 735, 978
SchI GAGTC 2 cut(s) 101, 937
ScrFI CCNGG 2 cut(s) 267, 519
SfaNI GCATC 3 cut(s) 307, 507, 795
SfcI CTRYAG 1 cut(s) 836
Sfr274I CTCGAG 2 cut(s) 149, 944
SgrAI CRCCGGYG 1 cut(s) 60
SgrDI CGTCGACG 1 cut(s) 125
SlaI CTCGAG 2 cut(s) 149, 944
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 4 cut(s) 149, 321, 729, 944
SmoI CTYRAG 4 cut(s) 149, 321, 729, 944
Sse9I AATT 4 cut(s) 54, 572, 594, 810
SsiI CCGC 3 cut(s) 27, 30, 1047
StyD4I CCNGG 2 cut(s) 265, 517
StyI CCWWGG 2 cut(s) 20, 1059
TaaI ACNGT 3 cut(s) 179, 538, 892
TaiI ACGT 1 cut(s) 253
TaqI TCGA 4 cut(s) 126, 150, 945, 981
TasI AATT 4 cut(s) 54, 572, 594, 810
TatI WGTACW 1 cut(s) 1089
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 5 cut(s) 154, 191, 411, 718, 853
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 2 cut(s) 541, 823
TseFI GTSAC 2 cut(s) 173, 455
TseI GCWGC 2 cut(s) 688, 832
Tsp45I GTSAC 2 cut(s) 173, 455
TspDTI ATGAA 4 cut(s) 403, 585, 921, 1098
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 2 cut(s) 541, 823
XapI RAATTY 1 cut(s) 572
XceI RCATGY 1 cut(s) 469
XhoI CTCGAG 2 cut(s) 149, 944
XmiI GTMKAC 1 cut(s) 126
XmnI GAANNNNTTC 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.