Rorug05G0491500

ATPase family associated with various cellular activities (AAA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006008
Physical Location & Seq
Forward (+)
67445152 .. 67446487
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0491500.1

Sequence Viewer

Length: 693 bp
ATGAAGCGTCACCACCAAGTTTTAGCAGCACAAAGGGGAGTGTTGAAGAATATTGACATTTGTGTGCCTTATCAAACTAGGAGTAGTTCTATGATTGTAATAGGAGAGAAGGTTCTAGAATTCCTAGTCCTATTCGGAATAGTTTTGGAAATGCTTGTATGCTCATTAGTATCAAAGGTGCCTTTGTCAGAAACTCTTCTGAAGCTTAAATCAGAACCAAACTTGAGCAATGAATGTCCAATATGTATTGAACCTCAAACAGTAGGAAGCTGGATGAATGAGGAAGGAAACATTTCTTTGAAGCTTATGGTTAGCAAATCTCAGAAGATAGTTTGCTATGCAGAAGCAGGGGAGGATTTTGTCAATTTACTTTTCAGTTTCTTGACTGTTCCACTCGGGTTTATAATAAAACAGATCCAGGATAGCTCGTCTTCTTTGAAAGGTAGCATTGAGCAGCTAAGTGTCCAGGATCTTGATGATGAATTCTTCAAATCAGATTACCAGAAGGAATTGAGTATGGCCTGGAGACCTCGAATTATTATGCACGCTACTGGACAGGTACTATTCACTGATATTGAGGAGCAAGTTGTGCAGGTCGGAAAGGAGGAGGCTTTTCGCCTTTTGGTAACATCTTTTCTTTGTGATTCTTCTCTCACCGGTGTCTTCCTTCGGCAGCCAAAGAGCAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_30

Protein Analysis

230

Amino Acids

25.87

Weight (kDa)

5.5

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 45 - 165 1.6e-25 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 583
AccB1I GGYRCC 1 cut(s) 178
AclWI GGATC 2 cut(s) 409, 477
AcsI RAATTY 2 cut(s) 119, 482
AcuI CTGAAG 1 cut(s) 221
AfaI GTAC 1 cut(s) 561
AgeI ACCGGT 1 cut(s) 656
AgsI TTSAA 5 cut(s) 46, 251, 301, 439, 490
AjnI CCWGG 3 cut(s) 417, 465, 521
AloI GAACNNNNNNTCC 2 cut(s) 96, 128
AluBI AGCT 5 cut(s) 205, 270, 304, 426, 457
AluI AGCT 5 cut(s) 205, 270, 304, 426, 457
Alw26I GTCTC 1 cut(s) 520
AlwI GGATC 2 cut(s) 409, 477
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 1 cut(s) 519
ApeKI GCWGC 3 cut(s) 26, 454, 673
ApoI RAATTY 2 cut(s) 119, 482
AsiGI ACCGGT 1 cut(s) 656
Asp700I GAANNNNTTC 2 cut(s) 195, 292
AsuHPI GGTGA 1 cut(s) 646
AvaI CYCGRG 1 cut(s) 395
BanI GGYRCC 1 cut(s) 178
BbsI GAAGAC 2 cut(s) 423, 655
BbvI GCAGC 3 cut(s) 38, 466, 685
BciT130I CCWGG 3 cut(s) 419, 467, 523
BcoDI GTCTC 1 cut(s) 520
BfaI CTAG 3 cut(s) 78, 116, 125
BfuAI ACCTGC 1 cut(s) 583
BisI GCNGC 3 cut(s) 27, 455, 674
BlsI GCNGC 3 cut(s) 28, 456, 675
Bme1390I CCNGG 3 cut(s) 419, 467, 523
BmeT110I CYCGRG 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 3 cut(s) 419, 467, 523
BpiI GAAGAC 2 cut(s) 423, 655
BpmI CTGGAG 1 cut(s) 544
BpuEI CTTGAG 1 cut(s) 244
BsaI GGTCTC 1 cut(s) 520
BsaWI WCCGGW 1 cut(s) 656
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 1 cut(s) 556
Bse3DI GCAATG 1 cut(s) 235
BseBI CCWGG 3 cut(s) 419, 467, 523
BseGI GGATG 1 cut(s) 279
BseMI GCAATG 1 cut(s) 235
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 556
BseRI GAGGAG 2 cut(s) 593, 620
BseXI GCAGC 3 cut(s) 38, 466, 685
BsgI GTGCAG 1 cut(s) 611
BshFI GGCC 1 cut(s) 521
BshNI GGYRCC 1 cut(s) 178
BshTI ACCGGT 1 cut(s) 656
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 657
BsmAI GTCTC 1 cut(s) 520
BsnI GGCC 1 cut(s) 521
Bso31I GGTCTC 1 cut(s) 520
BsoBI CYCGRG 1 cut(s) 395
Bsp143I GATC 2 cut(s) 414, 469
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 1 cut(s) 334
BspLI GGNNCC 1 cut(s) 180
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 2 cut(s) 409, 477
BspT107I GGYRCC 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 656
BsrI ACTGG 1 cut(s) 556
BssAI RCCGGY 1 cut(s) 656
BssMI GATC 2 cut(s) 414, 469
Bst2UI CCWGG 3 cut(s) 419, 467, 523
Bst4CI ACNGT 2 cut(s) 262, 388
Bst6I CTCTTC 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 546
BstDEI CTNAG 2 cut(s) 321, 458
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 2 cut(s) 417, 472
BstMAI GTCTC 1 cut(s) 520
BstMBI GATC 2 cut(s) 414, 469
BstMWI GCNNNNNNNGC 1 cut(s) 589
BstNI CCWGG 3 cut(s) 419, 467, 523
BstSCI CCNGG 3 cut(s) 417, 465, 521
BstV1I GCAGC 3 cut(s) 38, 466, 685
BstV2I GAAGAC 2 cut(s) 423, 655
BstX2I RGATCY 2 cut(s) 414, 469
BstYI RGATCY 2 cut(s) 414, 469
BsuRI GGCC 1 cut(s) 521
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 567
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 1 cut(s) 546
Cfr10I RCCGGY 1 cut(s) 656
Csp6I GTAC 1 cut(s) 560
CspAI ACCGGT 1 cut(s) 656
CviJI RGCY 8 cut(s) 205, 270, 304, 426, 457, 521, 611, 676
CviKI_1 RGCY 8 cut(s) 205, 270, 304, 426, 457, 521, 611, 676
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 2 cut(s) 321, 458
DpnI GATC 2 cut(s) 416, 471
DpnII GATC 2 cut(s) 414, 469
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 520
Eco57I CTGAAG 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 395
EcoRI GAATTC 2 cut(s) 119, 482
EcoRII CCWGG 3 cut(s) 417, 465, 521
FaiI YATR 8 cut(s) 92, 160, 244, 308, 339, 404, 518, 542
Fnu4HI GCNGC 3 cut(s) 27, 455, 674
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 3 cut(s) 27, 455, 674
FspBI CTAG 3 cut(s) 78, 116, 125
GluI GCNGC 3 cut(s) 27, 455, 674
GsuI CTGGAG 1 cut(s) 544
HaeIII GGCC 1 cut(s) 521
HapII CCGG 1 cut(s) 657
HindIII AAGCTT 2 cut(s) 203, 302
HinfI GANTC 1 cut(s) 644
HpaII CCGG 1 cut(s) 657
HphI GGTGA 1 cut(s) 646
Hpy188I TCNGA 7 cut(s) 137, 190, 201, 214, 324, 496, 599
Hpy188III TCNNGA 3 cut(s) 116, 382, 473
HpyAV CCTTC 4 cut(s) 103, 278, 499, 677
HpyCH4III ACNGT 2 cut(s) 262, 388
HpyCH4V TGCA 3 cut(s) 341, 544, 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 589
HpyF3I CTNAG 2 cut(s) 321, 458
Kzo9I GATC 2 cut(s) 414, 469
LmnI GCTCC 1 cut(s) 580
Lsp1109I GCAGC 3 cut(s) 38, 466, 685
MaeI CTAG 3 cut(s) 78, 116, 125
MaeIII GTNAC 2 cut(s) 8, 625
MalI GATC 2 cut(s) 416, 471
MboI GATC 2 cut(s) 414, 469
MboII GAAGA 7 cut(s) 58, 188, 337, 423, 478, 639, 655
MflI RGATCY 2 cut(s) 414, 469
MluCI AATT 5 cut(s) 119, 364, 482, 509, 534
MmeI TCCRAC 1 cut(s) 577
MnlI CCTC 7 cut(s) 264, 274, 346, 540, 571, 598, 601
MroXI GAANNNNTTC 2 cut(s) 195, 292
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 62
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 3 cut(s) 419, 467, 523
MvaI CCWGG 3 cut(s) 419, 467, 523
MwoI GCNNNNNNNGC 1 cut(s) 589
NdeII GATC 2 cut(s) 414, 469
NlaIV GGNNCC 1 cut(s) 180
NmuCI GTSAC 1 cut(s) 8
PdmI GAANNNNTTC 2 cut(s) 195, 292
PfeI GAWTC 1 cut(s) 644
PfoI TCCNGGA 2 cut(s) 417, 465
PinAI ACCGGT 1 cut(s) 656
PkrI GCNGC 3 cut(s) 28, 456, 675
PsiI TTATAA 1 cut(s) 404
Psp6I CCWGG 3 cut(s) 417, 465, 521
PspGI CCWGG 3 cut(s) 417, 465, 521
PspN4I GGNNCC 1 cut(s) 180
PsuI RGATCY 2 cut(s) 414, 469
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
RseI CAYNNNNRTG 1 cut(s) 62
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 3 cut(s) 27, 455, 674
Sau3AI GATC 2 cut(s) 414, 469
ScrFI CCNGG 3 cut(s) 419, 467, 523
SgrAI CRCCGGYG 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 5 cut(s) 119, 364, 482, 509, 534
SspI AATATT 1 cut(s) 52
SspMI CTAG 3 cut(s) 78, 116, 125
StyD4I CCNGG 3 cut(s) 417, 465, 521
TaaI ACNGT 2 cut(s) 262, 388
TaqI TCGA 1 cut(s) 532
TasI AATT 5 cut(s) 119, 364, 482, 509, 534
TfiI GAWTC 1 cut(s) 644
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 3 cut(s) 26, 454, 673
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 4 cut(s) 17, 246, 290, 495
TspRI CASTG 1 cut(s) 574
XapI RAATTY 2 cut(s) 119, 482
XbaI TCTAGA 1 cut(s) 115
XmnI GAANNNNTTC 2 cut(s) 195, 292
XspI CTAG 3 cut(s) 78, 116, 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.