Rorug05G0491600

ATPase family associated with various cellular activities (AAA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006008
Physical Location & Seq
Forward (+)
67448549 .. 67450173
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0491600.1

Sequence Viewer

Length: 1455 bp
ATGGCTCAAGAAGTATCAGATGCGAAGAATATCAGCTTGAAGGCATTGGTGGACAAGGGAAGAAACAGAGTAATCTTCGTAGAGGCCGATAATGATTTCATTGATGTTCTCTTCAGTTTTTTGACATTACCAATGGGAAAAATTGTCAAGCTTGCTCGCGACCATTCAACAGCACTGGAAATAGGTTGCATAAACAAATTGTATCAGAGTATTGAGAAGCTTGATGTGCACGTTTTCAGGTCCAATGTATGTAGAGACATGTTGTTACTTCCGCACTGTGCAGCTAACTGTCATTGCAAGAACCTCAAATTGAAAATTATTGACGATGTGGAGCCAACACGGTTTAGGTGCAATAATTACGCTTACGGTTGCTCCTGGTTAAGTTATTATGCAACGGTTCCTTGCCCTTCCTCACATACCGGGCGTTCTGAAGTATATCTTTCAGTGGACGATTCTCAAGTTGGAGGGGTATTTGTAAAAGACGGAGCCAGTGTTATAATAACTGATGATTTACAAGTGCTACCCCAATTAAGTGCAGCTAGCATTTCCTTGTTTACCAAGCTTGGAGCCACGAATAGCAATACCACTGAGGAGCTAACTTTTAATATTGGAGTCGAGCAGGTTTTGAATTTGCTTGTGAGCTCATTTGTATCCAAAACGCCATTCACTGAAACTCTGCTGAAGCCTAATCCTGTACCAAAATTTGGTTGTGTGAATTCCAGTCAAGGAAAATGTATTGAATCTCAAATGTCGGGGGACTCCATAGACGAGGAAAAAGAAAAAAACATCTCTGTCAAGCTCATAGTGAGTAAATCAAAGAATGTGGTTTGCTATGCAGAAGTACGAGAGGATTTTGTTAATTTGATCTTCAGTTTCTTGACTCTTCCACTTGGGTTTATATTAAAGCAGATGCTGGATTTCCCTTGGAAAGGATGTATTGATCAGTTGTGCAAGGGTGTCCTGGATTTTGATGAGCAATTTTTTAAGTCAAATCACCACAAGGAATTGCTATGCAATCCCAAACTTGTTCCTGGGTTCATCTTTGAGAACAATCTTTTAGGAATTGAGGAGGCCGTGTACTATAGGTTTTATGATAATAAGTATAAGTGGACTTCTGATAGTTCCATTATCCCTTCTAACCAGTCCTCAGTCGAAGTTAAATTTTTGGATCCCAAATCCCATGGTGATAAAGATAGAAATGCTAGGGGATTCTTAAAAGAACCAGCGATTTTCACAGTAACTGACAATTTGGATGTAAGGCGAGCATCTCCAATCTTTCAACTGTCTGTTCTTAATGATTTGAAGGTACATTTCACTGATATTGAGGACCGAACTGTGCATGTTGGCAAGAACGAGGCTTTGCGTCTTTTGGTGGCTTCTGTTGTTTCTGACTCAGCTCTAACCAATGCTTTCCTACGGAAGCCACAGGAGCCATCGATTAAGCGAGAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_30

Protein Analysis

484

Amino Acids

54.32

Weight (kDa)

6.11

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 9 - 450 1.5e-115 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 610
AccB7I CCANNNNNTGG 1 cut(s) 704
AccII CGCG 1 cut(s) 159
AciI CCGC 1 cut(s) 272
AclWI GGATC 2 cut(s) 1163, 1176
AcsI RAATTY 4 cut(s) 628, 701, 715, 1160
AcuI CTGAAG 4 cut(s) 97, 450, 701, 853
AfaI GTAC 4 cut(s) 696, 843, 1079, 1308
AfiI CCNNNNNNNGG 2 cut(s) 704, 929
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 7 cut(s) 40, 168, 313, 628, 740, 1280, 1303
AjnI CCWGG 3 cut(s) 374, 960, 1030
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
Alw21I GWGCWC 2 cut(s) 231, 644
Alw26I GTCTC 1 cut(s) 249
Alw44I GTGCAC 1 cut(s) 227
AlwI GGATC 2 cut(s) 1163, 1176
AlwNI CAGNNNCTG 2 cut(s) 913, 1241
AoxI GGCC 2 cut(s) 84, 1071
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 2 cut(s) 281, 536
ApoI RAATTY 4 cut(s) 628, 701, 715, 1160
AspS9I GGNCC 2 cut(s) 240, 1327
AsuC2I CCSGG 1 cut(s) 421
AsuHPI GGTGA 2 cut(s) 986, 1196
AsuNHI GCTAGC 1 cut(s) 539
AvaII GGWCC 2 cut(s) 240, 1327
BaeGI GKGCMC 1 cut(s) 231
BamHI GGATCC 1 cut(s) 1168
BanII GRGCYC 1 cut(s) 644
Bbv12I GWGCWC 2 cut(s) 231, 644
BbvI GCAGC 2 cut(s) 293, 548
BccI CCATC 1 cut(s) 1441
BceAI ACGGC 1 cut(s) 1058
BciT130I CCWGG 3 cut(s) 376, 962, 1032
BciVI GTATCC 1 cut(s) 661
BclI TGATCA 1 cut(s) 940
BcnI CCSGG 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 2 cut(s) 540, 1203
BfmI CTRYAG 1 cut(s) 1081
BfuAI ACCTGC 1 cut(s) 610
BfuI GTATCC 1 cut(s) 661
BisI GCNGC 2 cut(s) 282, 537
BlsI GCNGC 2 cut(s) 283, 538
Bme1390I CCNGG 4 cut(s) 376, 421, 962, 1032
Bme18I GGWCC 2 cut(s) 240, 1327
BmgT120I GGNCC 2 cut(s) 240, 1327
BmiI GGNNCC 6 cut(s) 333, 399, 487, 568, 1170, 1431
BmrFI CCNGG 4 cut(s) 376, 421, 962, 1032
BmsI GCATC 3 cut(s) 10, 900, 1274
BmtI GCTAGC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 441
BpuMI CCSGG 1 cut(s) 421
Bsa29I ATCGAT 1 cut(s) 1436
BsaJI CCNNGG 3 cut(s) 923, 1031, 1180
BsaXI ACNNNNNCTCC 6 cut(s) 356, 386, 477, 507, 1061, 1091
Bsc4I CCNNNNNNNGG 2 cut(s) 704, 929
Bse1I ACTGG 4 cut(s) 180, 489, 720, 1141
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 3 cut(s) 376, 962, 1032
BseCI ATCGAT 1 cut(s) 1436
BseDI CCNNGG 3 cut(s) 923, 1031, 1180
BseGI GGATG 2 cut(s) 938, 1258
BseLI CCNNNNNNNGG 2 cut(s) 704, 929
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 3 cut(s) 579, 1161, 1407
BseNI ACTGG 4 cut(s) 180, 489, 720, 1141
BseRI GAGGAG 2 cut(s) 605, 1082
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 2 cut(s) 293, 548
BsgI GTGCAG 2 cut(s) 300, 555
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 86, 1073
BshVI ATCGAT 1 cut(s) 1436
BsiHKAI GWGCWC 2 cut(s) 231, 644
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 1 cut(s) 770
BslI CCNNNNNNNGG 2 cut(s) 704, 929
BsmAI GTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 770
BsnI GGCC 2 cut(s) 86, 1073
Bsp1286I GDGCHC 2 cut(s) 231, 644
Bsp143I GATC 3 cut(s) 864, 940, 1168
Bsp19I CCATGG 1 cut(s) 1180
Bsp68I TCGCGA 1 cut(s) 159
BspACI CCGC 1 cut(s) 272
BspANI GGCC 2 cut(s) 86, 1073
BspCNI CTCAG 3 cut(s) 580, 1160, 1406
BspDI ATCGAT 1 cut(s) 1436
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 6 cut(s) 333, 399, 487, 568, 1170, 1431
BspMI ACCTGC 1 cut(s) 610
BspOI GCTAGC 1 cut(s) 543
BspPI GGATC 2 cut(s) 1163, 1176
BsrDI GCAATG 1 cut(s) 292
BsrI ACTGG 4 cut(s) 180, 489, 720, 1141
BssECI CCNNGG 3 cut(s) 923, 1031, 1180
BssMI GATC 3 cut(s) 864, 940, 1168
BssT1I CCWWGG 2 cut(s) 923, 1180
Bst2UI CCWGG 3 cut(s) 376, 962, 1032
Bst4CI ACNGT 8 cut(s) 278, 290, 342, 368, 397, 1237, 1284, 1336
Bst6I CTCTTC 2 cut(s) 116, 888
BstC8I GCNNGC 4 cut(s) 153, 157, 541, 1263
BstDEI CTNAG 3 cut(s) 588, 1147, 1393
BstDSI CCRYGG 1 cut(s) 1180
BstENI CCTNNNNNAGG 1 cut(s) 927
BstF5I GGATG 2 cut(s) 938, 1258
BstFNI CGCG 1 cut(s) 159
BstKTI GATC 3 cut(s) 867, 943, 1171
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 3 cut(s) 864, 940, 1168
BstMWI GCNNNNNNNGC 2 cut(s) 226, 1429
BstNI CCWGG 3 cut(s) 376, 962, 1032
BstNSI RCATGY 2 cut(s) 262, 1343
BstSCI CCNGG 4 cut(s) 374, 419, 960, 1030
BstSFI CTRYAG 1 cut(s) 1081
BstSLI GKGCMC 1 cut(s) 231
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 293, 548
BstX2I RGATCY 1 cut(s) 1168
BstYI RGATCY 1 cut(s) 1168
Bsu15I ATCGAT 1 cut(s) 1436
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 2 cut(s) 86, 1073
BsuTUI ATCGAT 1 cut(s) 1436
BtgI CCRYGG 1 cut(s) 1180
BtsCI GGATG 2 cut(s) 938, 1258
BtsIMutI CAGTG 7 cut(s) 173, 274, 450, 496, 585, 666, 1314
BtuMI TCGCGA 1 cut(s) 159
BveI ACCTGC 1 cut(s) 610
Cac8I GCNNGC 4 cut(s) 153, 157, 541, 1263
CaiI CAGNNNCTG 2 cut(s) 913, 1241
Cfr13I GGNCC 2 cut(s) 240, 1327
ClaI ATCGAT 1 cut(s) 1436
CseI GACGC 1 cut(s) 1352
Csp6I GTAC 4 cut(s) 695, 842, 1078, 1307
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviAII CATG 3 cut(s) 259, 1181, 1340
CviQI GTAC 4 cut(s) 695, 842, 1078, 1307
DdeI CTNAG 3 cut(s) 588, 1147, 1393
DpnI GATC 3 cut(s) 866, 942, 1170
DpnII GATC 3 cut(s) 864, 940, 1168
Eam1104I CTCTTC 2 cut(s) 116, 888
EarI CTCTTC 2 cut(s) 116, 888
Ecl136II GAGCTC 1 cut(s) 642
Eco130I CCWWGG 2 cut(s) 923, 1180
Eco24I GRGCYC 1 cut(s) 644
Eco47I GGWCC 2 cut(s) 240, 1327
Eco53kI GAGCTC 1 cut(s) 642
Eco57I CTGAAG 4 cut(s) 97, 450, 701, 853
EcoICRI GAGCTC 1 cut(s) 642
EcoNI CCTNNNNNAGG 1 cut(s) 927
EcoRI GAATTC 1 cut(s) 715
EcoRII CCWGG 3 cut(s) 374, 960, 1030
EcoT14I CCWWGG 2 cut(s) 923, 1180
EcoT38I GRGCYC 1 cut(s) 644
ErhI CCWWGG 2 cut(s) 923, 1180
FaeI CATG 3 cut(s) 262, 1184, 1343
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FaqI GGGAC 1 cut(s) 770
FatI CATG 3 cut(s) 258, 1180, 1339
FbaI TGATCA 1 cut(s) 940
Fnu4HI GCNGC 2 cut(s) 282, 537
FokI GGATG 2 cut(s) 945, 1265
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 2 cut(s) 282, 537
FspBI CTAG 2 cut(s) 540, 1203
GluI GCNGC 2 cut(s) 282, 537
HaeIII GGCC 2 cut(s) 86, 1073
HapII CCGG 1 cut(s) 420
HgaI GACGC 1 cut(s) 1352
Hin1II CATG 3 cut(s) 262, 1184, 1343
HindIII AAGCTT 3 cut(s) 149, 218, 560
HinfI GANTC 7 cut(s) 452, 612, 740, 758, 880, 1209, 1391
HpaII CCGG 1 cut(s) 420
HphI GGTGA 2 cut(s) 986, 1196
Hpy166II GTNNAC 6 cut(s) 52, 229, 448, 555, 1078, 1110
Hpy188I TCNGA 5 cut(s) 19, 207, 430, 1117, 1390
Hpy188III TCNNGA 3 cut(s) 8, 158, 877
Hpy8I GTNNAC 6 cut(s) 52, 229, 448, 555, 1078, 1110
HpyAV CCTTC 4 cut(s) 34, 417, 1143, 1297
HpyCH4III ACNGT 8 cut(s) 278, 290, 342, 368, 397, 1237, 1284, 1336
HpyCH4IV ACGT 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 1429
HpyF3I CTNAG 3 cut(s) 588, 1147, 1393
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 3 cut(s) 262, 1184, 1343
Ksp22I TGATCA 1 cut(s) 940
Kzo9I GATC 3 cut(s) 864, 940, 1168
LmnI GCTCC 6 cut(s) 331, 377, 485, 566, 592, 1429
Lsp1109I GCAGC 2 cut(s) 293, 548
LweI GCATC 3 cut(s) 10, 900, 1274
MaeI CTAG 2 cut(s) 540, 1203
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 264, 1237
MalI GATC 3 cut(s) 866, 942, 1170
MboI GATC 3 cut(s) 864, 940, 1168
MboII GAAGA 6 cut(s) 37, 67, 72, 103, 859, 875
MflI RGATCY 1 cut(s) 1168
MhlI GDGCHC 2 cut(s) 231, 644
MlyI GAGTC 4 cut(s) 621, 752, 874, 1385
MmeI TCCRAC 1 cut(s) 442
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 4 cut(s) 376, 421, 962, 1032
MvaI CCWGG 3 cut(s) 376, 962, 1032
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 226, 1429
NciI CCSGG 1 cut(s) 421
NcoI CCATGG 1 cut(s) 1180
NdeII GATC 3 cut(s) 864, 940, 1168
NheI GCTAGC 1 cut(s) 539
NlaIII CATG 3 cut(s) 262, 1184, 1343
NlaIV GGNNCC 6 cut(s) 333, 399, 487, 568, 1170, 1431
NruI TCGCGA 1 cut(s) 159
NspI RCATGY 2 cut(s) 262, 1343
PciI ACATGT 1 cut(s) 258
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 3 cut(s) 452, 740, 1209
PflMI CCANNNNNTGG 1 cut(s) 704
PfoI TCCNGGA 1 cut(s) 960
PkrI GCNGC 2 cut(s) 283, 538
PleI GAGTC 4 cut(s) 620, 752, 874, 1385
PpsI GAGTC 4 cut(s) 620, 752, 874, 1385
PscI ACATGT 1 cut(s) 258
PsiI TTATAA 1 cut(s) 497
Psp124BI GAGCTC 1 cut(s) 644
Psp6I CCWGG 3 cut(s) 374, 960, 1030
PspGI CCWGG 3 cut(s) 374, 960, 1030
PspN4I GGNNCC 6 cut(s) 333, 399, 487, 568, 1170, 1431
PspPI GGNCC 2 cut(s) 240, 1327
PstNI CAGNNNCTG 2 cut(s) 913, 1241
PsuI RGATCY 1 cut(s) 1168
RruI TCGCGA 1 cut(s) 159
RsaI GTAC 4 cut(s) 696, 843, 1079, 1308
RsaNI GTAC 4 cut(s) 695, 842, 1078, 1307
SacI GAGCTC 1 cut(s) 644
SatI GCNGC 2 cut(s) 282, 537
Sau3AI GATC 3 cut(s) 864, 940, 1168
Sau96I GGNCC 2 cut(s) 240, 1327
SchI GAGTC 4 cut(s) 621, 752, 874, 1385
ScrFI CCNGG 4 cut(s) 376, 421, 962, 1032
SduI GDGCHC 2 cut(s) 231, 644
SfaNI GCATC 3 cut(s) 10, 900, 1274
SfcI CTRYAG 1 cut(s) 1081
SinI GGWCC 2 cut(s) 240, 1327
SmlI CTYRAG 2 cut(s) 6, 456
SmoI CTYRAG 2 cut(s) 6, 456
SsiI CCGC 1 cut(s) 272
SspI AATATT 1 cut(s) 607
SspMI CTAG 2 cut(s) 540, 1203
SstI GAGCTC 1 cut(s) 644
StyD4I CCNGG 4 cut(s) 374, 419, 960, 1030
StyI CCWWGG 2 cut(s) 923, 1180
TaaI ACNGT 8 cut(s) 278, 290, 342, 368, 397, 1237, 1284, 1336
TaiI ACGT 1 cut(s) 234
TaqI TCGA 3 cut(s) 615, 1152, 1436
TaqII GACCGA 1 cut(s) 1344
TatI WGTACW 1 cut(s) 1077
TfiI GAWTC 3 cut(s) 452, 740, 1209
TscAI CASTG 7 cut(s) 180, 281, 450, 496, 592, 673, 1321
TseI GCWGC 2 cut(s) 281, 536
TspDTI ATGAA 2 cut(s) 88, 1027
TspGWI ACGGA 2 cut(s) 498, 1432
TspRI CASTG 7 cut(s) 180, 281, 450, 496, 592, 673, 1321
Van91I CCANNNNNTGG 1 cut(s) 704
VneI GTGCAC 1 cut(s) 227
VpaK11BI GGWCC 2 cut(s) 240, 1327
XagI CCTNNNNNAGG 1 cut(s) 927
XapI RAATTY 4 cut(s) 628, 701, 715, 1160
XceI RCATGY 2 cut(s) 262, 1343
XspI CTAG 2 cut(s) 540, 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.