AT1G35160

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007005
Physical Location & Seq
Forward (+)
12867081 .. 12868890
1810 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G35160.1

Sequence Viewer

Length: 804 bp
ATGGCGGCACCACCAGCATCATCCTCGGCGAGGGAAGAGTTCGTGTACCTCGCAAAGCTCGCAGAGCAAGCGGAACGTTACGAAGAAATGGTTGAATTCATGGAAAAAGTCGCTGAAGCCGTTGACAAAGACGAACTCACCGTCGAAGAACGTAATCTCCTCTCCGTCGCTTACAAAAACGTAATCGGCGCTCGTCGTGCTTCCTGGAGAATCATCTCTTCCATTGAACAAAAAGAAGAGAGTCGTGGTAACGATGACCATGTGACCACGATCCGTGATTACAGAAGCAAGATCGAATCTGAGTTATCGAAAATCTGTGACGGTATTCTTAAGCTTCTTGATACTAGACTTGTTCCTGCTTCTGCTAATGGAGATTCTAAGGTTTTTTACCTTAAGATGAAGGGAGATTATCATAGGTATTTGGCTGAGTTTAAGACTGGTCAAGAGAGGAAAGATGCTGCTGAACATACTCTCACCGCTTACAAAGCTGCTCAGGATATTGCTAATGCTGAATTGGCTCCAACGCATCCGATTCGTCTTGGTTTGGCTTTGAATTTCTCTGTGTTTTACTATGAGATTCTTAATTCTCCAGATCGTGCTTGTAATCTCGCTAAGCAGGCGTTTGATGAAGCCATTGCTGAGTTAGATACTCTTGGAGAGGAGTCATACAAGGATAGTACCTTGATCATGCAGCTTCTTCGTGACAATCTTACTCTCTGGACTTCTGACATGCAGGACGAAAGTCCGGAGGAGATTAAAGAAGCAGCAGCACCAAAGCCTGCTGAAGAACAGAAGGAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

267

Amino Acids

30.19

Weight (kDa)

4.79

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 241 3.3e-106 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 745
AciI CCGC 3 cut(s) 5, 71, 477
AclI AACGTT 1 cut(s) 76
AclWI GGATC 1 cut(s) 265
AcsI RAATTY 2 cut(s) 95, 553
AcuI CTGAAG 2 cut(s) 135, 804
AfaI GTAC 2 cut(s) 47, 679
AfiI CCNNNNNNNGG 1 cut(s) 30
AflII CTTAAG 2 cut(s) 329, 392
AgsI TTSAA 3 cut(s) 95, 227, 553
AjnI CCWGG 1 cut(s) 203
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
AluBI AGCT 4 cut(s) 58, 334, 488, 694
AluI AGCT 4 cut(s) 58, 334, 488, 694
AlwI GGATC 1 cut(s) 265
Aor13HI TCCGGA 1 cut(s) 745
ApeKI GCWGC 5 cut(s) 458, 488, 691, 764, 767
ApoI RAATTY 2 cut(s) 95, 553
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AspLEI GCGC 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 130, 466
BanI GGYRCC 1 cut(s) 7
BbvI GCAGC 5 cut(s) 445, 475, 703, 776, 779
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 6 cut(s) 6, 40, 515, 549, 680, 714
BciT130I CCWGG 1 cut(s) 205
BclI TGATCA 1 cut(s) 684
BfaI CTAG 1 cut(s) 345
BfoI RGCGCY 1 cut(s) 192
BfrI CTTAAG 2 cut(s) 329, 392
BglII AGATCT 1 cut(s) 798
BisI GCNGC 6 cut(s) 6, 459, 489, 692, 765, 768
BlpI GCTNAGC 1 cut(s) 612
BlsI GCNGC 6 cut(s) 7, 460, 490, 693, 766, 769
Bme1390I CCNGG 1 cut(s) 205
BmiI GGNNCC 2 cut(s) 9, 519
BmrFI CCNGG 1 cut(s) 205
BmsI GCATC 3 cut(s) 26, 445, 535
BoxI GACNNNNGTC 1 cut(s) 741
BpmI CTGGAG 2 cut(s) 226, 573
Bpu10I CCTNAGC 1 cut(s) 492
Bpu1102I GCTNAGC 1 cut(s) 612
BsaJI CCNNGG 1 cut(s) 24
BsaWI WCCGGW 1 cut(s) 745
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 442
Bse3DI GCAATG 1 cut(s) 633
BseAI TCCGGA 1 cut(s) 745
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 2 cut(s) 20, 526
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 4 cut(s) 291, 417, 506, 630
BseNI ACTGG 1 cut(s) 442
BseRI GAGGAG 3 cut(s) 149, 674, 764
BseXI GCAGC 5 cut(s) 445, 475, 703, 776, 779
BshNI GGYRCC 1 cut(s) 7
BsiSI CCGG 1 cut(s) 746
BslI CCNNNNNNNGG 1 cut(s) 30
Bsp13I TCCGGA 1 cut(s) 745
Bsp143I GATC 5 cut(s) 270, 291, 592, 684, 798
Bsp1720I GCTNAGC 1 cut(s) 612
BspACI CCGC 3 cut(s) 5, 71, 477
BspCNI CTCAG 4 cut(s) 292, 418, 505, 631
BspEI TCCGGA 1 cut(s) 745
BspLI GGNNCC 2 cut(s) 9, 519
BspPI GGATC 1 cut(s) 265
BspT107I GGYRCC 1 cut(s) 7
BspTI CTTAAG 2 cut(s) 329, 392
BsrDI GCAATG 1 cut(s) 633
BsrI ACTGG 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 5 cut(s) 270, 291, 592, 684, 798
Bst2UI CCWGG 1 cut(s) 205
Bst4CI ACNGT 2 cut(s) 142, 323
Bst6I CTCTTC 3 cut(s) 30, 223, 231
BstAFI CTTAAG 2 cut(s) 329, 392
BstC8I GCNNGC 4 cut(s) 60, 69, 618, 780
BstDEI CTNAG 6 cut(s) 300, 378, 426, 492, 612, 639
BstENI CCTNNNNNAGG 1 cut(s) 28
BstF5I GGATG 2 cut(s) 20, 526
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 5 cut(s) 273, 294, 595, 687, 801
BstMBI GATC 5 cut(s) 270, 291, 592, 684, 798
BstMWI GCNNNNNNNGC 8 cut(s) 14, 59, 64, 68, 197, 485, 515, 617
BstNI CCWGG 1 cut(s) 205
BstNSI RCATGY 1 cut(s) 733
BstPAI GACNNNNGTC 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 203
BstV1I GCAGC 5 cut(s) 445, 475, 703, 776, 779
BstX2I RGATCY 1 cut(s) 798
BstYI RGATCY 1 cut(s) 798
BtsCI GGATG 2 cut(s) 20, 526
Cac8I GCNNGC 4 cut(s) 60, 69, 618, 780
CfoI GCGC 1 cut(s) 191
Csp6I GTAC 2 cut(s) 46, 678
CviAII CATG 4 cut(s) 100, 260, 688, 730
CviQI GTAC 2 cut(s) 46, 678
DdeI CTNAG 6 cut(s) 300, 378, 426, 492, 612, 639
DpnI GATC 5 cut(s) 272, 293, 594, 686, 800
DpnII GATC 5 cut(s) 270, 291, 592, 684, 798
Eam1104I CTCTTC 3 cut(s) 30, 223, 231
EarI CTCTTC 3 cut(s) 30, 223, 231
Eco57I CTGAAG 2 cut(s) 135, 804
EcoNI CCTNNNNNAGG 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 203
FaeI CATG 4 cut(s) 103, 263, 691, 733
FaiI YATR 8 cut(s) 101, 261, 414, 468, 573, 667, 689, 731
FatI CATG 4 cut(s) 99, 259, 687, 729
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 6 cut(s) 6, 459, 489, 692, 765, 768
FokI GGATG 2 cut(s) 7, 513
Fsp4HI GCNGC 6 cut(s) 6, 459, 489, 692, 765, 768
FspBI CTAG 1 cut(s) 345
GlaI GCGC 1 cut(s) 190
GluI GCNGC 6 cut(s) 6, 459, 489, 692, 765, 768
GsuI CTGGAG 2 cut(s) 226, 573
HaeII RGCGCY 1 cut(s) 192
HapII CCGG 1 cut(s) 746
HhaI GCGC 1 cut(s) 191
Hin1II CATG 4 cut(s) 103, 263, 691, 733
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 7 cut(s) 210, 241, 296, 374, 532, 577, 662
HpaII CCGG 1 cut(s) 746
HphI GGTGA 2 cut(s) 130, 466
Hpy166II GTNNAC 2 cut(s) 46, 124
Hpy188I TCNGA 3 cut(s) 301, 531, 727
Hpy188III TCNNGA 7 cut(s) 338, 443, 494, 590, 701, 718, 746
Hpy8I GTNNAC 2 cut(s) 46, 124
Hpy99I CGWCG 3 cut(s) 146, 170, 198
HpyAV CCTTC 2 cut(s) 394, 787
HpyCH4III ACNGT 2 cut(s) 142, 323
HpyCH4IV ACGT 3 cut(s) 76, 151, 180
HpyCH4V TGCA 2 cut(s) 691, 733
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 59, 64, 68, 197, 485, 515, 617
HpyF3I CTNAG 6 cut(s) 300, 378, 426, 492, 612, 639
HpySE526I ACGT 3 cut(s) 76, 151, 180
Hsp92II CATG 4 cut(s) 103, 263, 691, 733
HspAI GCGC 1 cut(s) 189
Kpn2I TCCGGA 1 cut(s) 745
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 5 cut(s) 270, 291, 592, 684, 798
LmnI GCTCC 1 cut(s) 523
Lsp1109I GCAGC 5 cut(s) 445, 475, 703, 776, 779
LweI GCATC 3 cut(s) 26, 445, 535
MaeI CTAG 1 cut(s) 345
MaeII ACGT 3 cut(s) 76, 151, 180
MaeIII GTNAC 5 cut(s) 77, 248, 262, 317, 701
MalI GATC 5 cut(s) 272, 293, 594, 686, 800
MboI GATC 5 cut(s) 270, 291, 592, 684, 798
MboII GAAGA 7 cut(s) 47, 95, 158, 210, 248, 689, 797
MflI RGATCY 1 cut(s) 798
MluCI AATT 4 cut(s) 95, 512, 553, 583
MlyI GAGTC 2 cut(s) 250, 671
MmeI TCCRAC 1 cut(s) 545
MnlI CCTC 7 cut(s) 24, 34, 59, 170, 441, 652, 742
MroI TCCGGA 1 cut(s) 745
MseI TTAA 5 cut(s) 330, 393, 432, 582, 756
MspCI CTTAAG 2 cut(s) 329, 392
MspI CCGG 1 cut(s) 746
MspR9I CCNGG 1 cut(s) 205
MvaI CCWGG 1 cut(s) 205
MwoI GCNNNNNNNGC 8 cut(s) 14, 59, 64, 68, 197, 485, 515, 617
NdeII GATC 5 cut(s) 270, 291, 592, 684, 798
NlaIII CATG 4 cut(s) 103, 263, 691, 733
NlaIV GGNNCC 2 cut(s) 9, 519
NmeAIII GCCGAG 1 cut(s) 5
NmuCI GTSAC 3 cut(s) 262, 317, 701
NspI RCATGY 1 cut(s) 733
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 138
PfeI GAWTC 5 cut(s) 210, 296, 374, 532, 577
PfoI TCCNGGA 1 cut(s) 203
PkrI GCNGC 6 cut(s) 7, 460, 490, 693, 766, 769
PleI GAGTC 2 cut(s) 249, 670
PpsI GAGTC 2 cut(s) 249, 670
PshAI GACNNNNGTC 1 cut(s) 741
Psp1406I AACGTT 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 203
PspGI CCWGG 1 cut(s) 203
PspN4I GGNNCC 2 cut(s) 9, 519
PsuI RGATCY 1 cut(s) 798
RsaI GTAC 2 cut(s) 47, 679
RsaNI GTAC 2 cut(s) 46, 678
SaqAI TTAA 5 cut(s) 330, 393, 432, 582, 756
SatI GCNGC 6 cut(s) 6, 459, 489, 692, 765, 768
Sau3AI GATC 5 cut(s) 270, 291, 592, 684, 798
SchI GAGTC 2 cut(s) 250, 671
ScrFI CCNGG 1 cut(s) 205
SfaNI GCATC 3 cut(s) 26, 445, 535
SmlI CTYRAG 2 cut(s) 329, 392
SmoI CTYRAG 2 cut(s) 329, 392
Sse9I AATT 4 cut(s) 95, 512, 553, 583
SsiI CCGC 3 cut(s) 5, 71, 477
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 203
TaaI ACNGT 2 cut(s) 142, 323
TaiI ACGT 3 cut(s) 79, 154, 183
TaqI TCGA 3 cut(s) 144, 294, 308
TasI AATT 4 cut(s) 95, 512, 553, 583
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 5 cut(s) 210, 296, 374, 532, 577
Tru1I TTAA 5 cut(s) 330, 393, 432, 582, 756
Tru9I TTAA 5 cut(s) 330, 393, 432, 582, 756
TseFI GTSAC 3 cut(s) 262, 317, 701
TseI GCWGC 5 cut(s) 458, 488, 691, 764, 767
Tsp45I GTSAC 3 cut(s) 262, 317, 701
TspDTI ATGAA 3 cut(s) 88, 413, 642
TspGWI ACGGA 2 cut(s) 154, 263
Vha464I CTTAAG 2 cut(s) 329, 392
XagI CCTNNNNNAGG 1 cut(s) 28
XapI RAATTY 2 cut(s) 95, 553
XceI RCATGY 1 cut(s) 733
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.