AT4G09000

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007005
Physical Location & Seq
Forward (+)
5775210 .. 5777481
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G09000.2

Sequence Viewer

Length: 804 bp
ATGGCGACACCAGGAGCTTCCTCAGCAAGAGATGAATTCGTATACATGGCGAAACTCGCCGAACAAGCAGAGCGTTACGAAGAAATGGTTGAATTCATGGAGAAAGTCGCGAAAGCTGTTGACAAAGACGAACTCACCGTCGAAGAACGTAATCTCCTCTCCGTCGCTTACAAAAACGTCATCGGAGCTCGTCGTGCTTCGTGGAGAATCATTTCATCGATCGAACAAAAGGAGGAGTCTCGCGGTAACGATGACCACGTTTCGTTGATCCGTGACTACAGAAGCAAAATCGAAACGGAACTTTCTGATATCTGTGACGGAATCCTTAAGCTTCTCGATACGATTCTCGTTCCCGCTGCTGCTTCTGGAGATTCGAAGGTGTTTTATCTGAAGATGAAAGGTGATTATCATAGGTACTTGGCTGAGTTCAAATCTGGTCAAGAGAGGAAAGATGCTGCTGAACATACTCTTACTGCTTACAAAGCTGCTCAGGATATTGCTAATTCTGAATTGGCTCCAACGCATCCGATTCGTCTTGGTCTTGCGTTGAACTTCTCTGTGTTTTACTATGAGATTCTCAATTCTCCAGATCGTGCTTGTAATCTCGCTAAGCAGGCGTTTGATGAAGCAATTGCTGAATTGGATACTCTTGGTGAAGAGTCATACAAGGACAGTACTTTGATTATGCAGCTTCTTCGTGATAACCTCACTCTCTGGACTTCTGATATGCAGGACGATGTTGCTGATGACATCAAAGAAGCAGCACCAGCAGCAGCAAAACCCGCTGACGAGCAACAATCCTAA

Protein Analysis

267

Amino Acids

29.93

Weight (kDa)

4.68

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 16 - 240 1.5e-106 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 42
AccII CGCG 2 cut(s) 110, 243
AciI CCGC 3 cut(s) 243, 354, 783
AclWI GGATC 1 cut(s) 262
AcsI RAATTY 2 cut(s) 35, 92
AcuI CTGAAG 1 cut(s) 410
AfaI GTAC 2 cut(s) 416, 676
AflII CTTAAG 1 cut(s) 326
AgsI TTSAA 3 cut(s) 92, 430, 550
AjnI CCWGG 1 cut(s) 10
AloI GAACNNNNNNTCC 2 cut(s) 138, 170
AluBI AGCT 6 cut(s) 17, 116, 188, 331, 485, 691
AluI AGCT 6 cut(s) 17, 116, 188, 331, 485, 691
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 262
ApeKI GCWGC 8 cut(s) 356, 359, 455, 485, 688, 761, 770, 773
ApoI RAATTY 2 cut(s) 35, 92
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
Asp700I GAANNNNTTC 1 cut(s) 211
AsuHPI GGTGA 3 cut(s) 127, 413, 665
AsuII TTCGAA 1 cut(s) 374
BanII GRGCYC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 190
BbvCI CCTCAGC 1 cut(s) 22
BbvI GCAGC 8 cut(s) 343, 346, 442, 472, 700, 773, 782, 785
BcgI CGANNNNNNTGC 4 cut(s) 512, 546, 677, 711
BciT130I CCWGG 1 cut(s) 12
BciVI GTATCC 1 cut(s) 637
BcoDI GTCTC 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 277
BfrI CTTAAG 1 cut(s) 326
BfuI GTATCC 1 cut(s) 637
BisI GCNGC 8 cut(s) 357, 360, 456, 486, 689, 762, 771, 774
BlpI GCTNAGC 1 cut(s) 609
BlsI GCNGC 8 cut(s) 358, 361, 457, 487, 690, 763, 772, 775
BmcAI AGTACT 1 cut(s) 676
Bme1390I CCNGG 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 2 cut(s) 442, 532
BpmI CTGGAG 2 cut(s) 387, 570
Bpu10I CCTNAGC 2 cut(s) 22, 489
Bpu1102I GCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 374
Bsa29I ATCGAT 1 cut(s) 218
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
BseBI CCWGG 1 cut(s) 12
BseCI ATCGAT 1 cut(s) 218
BseGI GGATG 1 cut(s) 523
BseMII CTCAG 3 cut(s) 36, 414, 503
BseRI GAGGAG 2 cut(s) 146, 248
BseXI GCAGC 8 cut(s) 343, 346, 442, 472, 700, 773, 782, 785
Bsh1236I CGCG 2 cut(s) 110, 243
Bsh1285I CGRYCG 1 cut(s) 222
BshVI ATCGAT 1 cut(s) 218
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 190
BsmAI GTCTC 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 190
Bsp143I GATC 3 cut(s) 219, 267, 589
Bsp1720I GCTNAGC 1 cut(s) 609
Bsp68I TCGCGA 1 cut(s) 110
BspACI CCGC 3 cut(s) 243, 354, 783
BspCNI CTCAG 3 cut(s) 35, 415, 502
BspDI ATCGAT 1 cut(s) 218
BspFNI CGCG 2 cut(s) 110, 243
BspLI GGNNCC 1 cut(s) 516
BspPI GGATC 1 cut(s) 262
BspT104I TTCGAA 1 cut(s) 374
BspTI CTTAAG 1 cut(s) 326
BssMI GATC 3 cut(s) 219, 267, 589
BssNAI GTATAC 1 cut(s) 43
Bst1107I GTATAC 1 cut(s) 43
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 2 cut(s) 139, 674
Bst6I CTCTTC 1 cut(s) 651
BstAFI CTTAAG 1 cut(s) 326
BstBI TTCGAA 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 615
BstDEI CTNAG 4 cut(s) 22, 423, 489, 609
BstF5I GGATG 1 cut(s) 523
BstFNI CGCG 2 cut(s) 110, 243
BstKTI GATC 3 cut(s) 222, 270, 592
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 3 cut(s) 219, 267, 589
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 9 cut(s) 23, 56, 65, 194, 482, 614, 767, 770, 782
BstNI CCWGG 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 277
BstUI CGCG 2 cut(s) 110, 243
BstV1I GCAGC 8 cut(s) 343, 346, 442, 472, 700, 773, 782, 785
BstZ17I GTATAC 1 cut(s) 43
Bsu15I ATCGAT 1 cut(s) 218
BsuI GTATCC 1 cut(s) 637
BsuTUI ATCGAT 1 cut(s) 218
BtsCI GGATG 1 cut(s) 523
BtuMI TCGCGA 1 cut(s) 110
Cac8I GCNNGC 1 cut(s) 615
ClaI ATCGAT 1 cut(s) 218
Csp6I GTAC 2 cut(s) 415, 675
CviAII CATG 2 cut(s) 46, 97
CviJI RGCY 8 cut(s) 17, 116, 188, 331, 422, 485, 515, 691
CviKI_1 RGCY 8 cut(s) 17, 116, 188, 331, 422, 485, 515, 691
CviQI GTAC 2 cut(s) 415, 675
DdeI CTNAG 4 cut(s) 22, 423, 489, 609
DpnI GATC 3 cut(s) 221, 269, 591
DpnII GATC 3 cut(s) 219, 267, 589
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Ecl136II GAGCTC 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 190
Eco32I GATATC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 188
EcoRI GAATTC 2 cut(s) 35, 92
EcoRII CCWGG 1 cut(s) 10
EcoRV GATATC 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 190
FaeI CATG 2 cut(s) 49, 100
FaiI YATR 9 cut(s) 43, 47, 98, 411, 465, 570, 664, 686, 728
FatI CATG 2 cut(s) 45, 96
FauI CCCGC 2 cut(s) 361, 790
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 8 cut(s) 357, 360, 456, 486, 689, 762, 771, 774
FokI GGATG 1 cut(s) 510
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 8 cut(s) 357, 360, 456, 486, 689, 762, 771, 774
GluI GCNGC 8 cut(s) 357, 360, 456, 486, 689, 762, 771, 774
GsuI CTGGAG 2 cut(s) 387, 570
Hin1II CATG 2 cut(s) 49, 100
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 8 cut(s) 207, 236, 321, 343, 371, 529, 574, 659
HphI GGTGA 3 cut(s) 127, 413, 665
Hpy166II GTNNAC 2 cut(s) 43, 121
Hpy188I TCNGA 6 cut(s) 185, 307, 390, 508, 528, 724
Hpy188III TCNNGA 8 cut(s) 109, 335, 366, 440, 491, 587, 698, 715
Hpy8I GTNNAC 2 cut(s) 43, 121
Hpy99I CGWCG 3 cut(s) 143, 167, 195
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 2 cut(s) 139, 674
HpyCH4IV ACGT 3 cut(s) 148, 177, 258
HpyCH4V TGCA 2 cut(s) 688, 730
HpyF10VI GCNNNNNNNGC 9 cut(s) 23, 56, 65, 194, 482, 614, 767, 770, 782
HpyF3I CTNAG 4 cut(s) 22, 423, 489, 609
HpySE526I ACGT 3 cut(s) 148, 177, 258
Hsp92II CATG 2 cut(s) 49, 100
Kzo9I GATC 3 cut(s) 219, 267, 589
LmnI GCTCC 3 cut(s) 14, 185, 520
LpnPI CCDG 9 cut(s) 24, 351, 420, 476, 599, 600, 700, 716, 780
Lsp1109I GCAGC 8 cut(s) 343, 346, 442, 472, 700, 773, 782, 785
LweI GCATC 2 cut(s) 442, 532
MaeII ACGT 3 cut(s) 148, 177, 258
MaeIII GTNAC 4 cut(s) 74, 245, 272, 314
MalI GATC 3 cut(s) 221, 269, 591
MboI GATC 3 cut(s) 219, 267, 589
MboII GAAGA 5 cut(s) 92, 155, 403, 668, 686
MfeI CAATTG 1 cut(s) 630
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 7 cut(s) 35, 92, 502, 509, 580, 630, 638
MlyI GAGTC 2 cut(s) 245, 668
MmeI TCCRAC 1 cut(s) 542
MnlI CCTC 5 cut(s) 31, 167, 226, 438, 716
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 1 cut(s) 327
MspA1I CMGCKG 2 cut(s) 356, 785
MspCI CTTAAG 1 cut(s) 326
MspR9I CCNGG 1 cut(s) 12
MunI CAATTG 1 cut(s) 630
MvaI CCWGG 1 cut(s) 12
MvnI CGCG 2 cut(s) 110, 243
MwoI GCNNNNNNNGC 9 cut(s) 23, 56, 65, 194, 482, 614, 767, 770, 782
NdeII GATC 3 cut(s) 219, 267, 589
NlaIII CATG 2 cut(s) 49, 100
NlaIV GGNNCC 1 cut(s) 516
NmuCI GTSAC 2 cut(s) 272, 314
NruI TCGCGA 1 cut(s) 110
NspV TTCGAA 1 cut(s) 374
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 255
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 6 cut(s) 207, 321, 343, 371, 529, 574
PkrI GCNGC 8 cut(s) 358, 361, 457, 487, 690, 763, 772, 775
Ple19I CGATCG 1 cut(s) 222
PleI GAGTC 2 cut(s) 244, 667
PpsI GAGTC 2 cut(s) 244, 667
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 516
PvuI CGATCG 1 cut(s) 222
RruI TCGCGA 1 cut(s) 110
RsaI GTAC 2 cut(s) 416, 676
RsaNI GTAC 2 cut(s) 415, 675
SacI GAGCTC 1 cut(s) 190
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 8 cut(s) 357, 360, 456, 486, 689, 762, 771, 774
Sau3AI GATC 3 cut(s) 219, 267, 589
ScaI AGTACT 1 cut(s) 676
SchI GAGTC 2 cut(s) 245, 668
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 1 cut(s) 190
SfaNI GCATC 2 cut(s) 442, 532
SfcI CTRYAG 1 cut(s) 277
SfuI TTCGAA 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 7 cut(s) 35, 92, 502, 509, 580, 630, 638
SsiI CCGC 3 cut(s) 243, 354, 783
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 1 cut(s) 10
TaaI ACNGT 2 cut(s) 139, 674
TaiI ACGT 3 cut(s) 151, 180, 261
TaqI TCGA 6 cut(s) 141, 218, 222, 291, 336, 374
TasI AATT 7 cut(s) 35, 92, 502, 509, 580, 630, 638
TatI WGTACW 1 cut(s) 674
TfiI GAWTC 6 cut(s) 207, 321, 343, 371, 529, 574
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseFI GTSAC 2 cut(s) 272, 314
TseI GCWGC 8 cut(s) 356, 359, 455, 485, 688, 761, 770, 773
Tsp45I GTSAC 2 cut(s) 272, 314
TspDTI ATGAA 5 cut(s) 48, 85, 204, 410, 639
TspGWI ACGGA 4 cut(s) 151, 260, 311, 333
Vha464I CTTAAG 1 cut(s) 326
XapI RAATTY 2 cut(s) 35, 92
XmiI GTMKAC 1 cut(s) 42
XmnI GAANNNNTTC 1 cut(s) 211
ZrmI AGTACT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.