MD00G1018600.v1.1

14-3-3-like protein 16R

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007005
Physical Location & Seq
Reverse (-)
3143713 .. 3146570
2858 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1018600.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGCAGCCTCATCCCCACGCGAAGAGATCGTGTACGTCGCCAAGCTCGCCGAGCAAGCCGAGCGATACGAGGAGATGGTTGAGTTCATGGAGAAGGTCGTCTCCTCCCTCCCTGAAGGCGAAGAGCCCACCGTCGAAGAGCGCAACCTCCTCTCCGTCGCCTACAAGAACGTGATCGGCGCCCGCCGTGCCTCGTGGCGCATCGTCTCCTCCATCGAGCAGAAGGAGGAGAGCCGCGGCAACAGCGACCACGTCGCCACTATCAAGGAGTACAGGGCTCGGATCGAGAACGAGCTCTCCAACATCTGCGGTGGGATTCTCAAGGTTCTCGAATCCAAGCTTGTTCCTGCGGCAAACGTTGGAGAGTCCAAGGTGTTTTATTTGAAGATGAAGGGAGATTACCATAGGTATTTGGCTGAGTTCAAAACTGGAGACGAGCGCAAGGAGGCCGCTGAGAACACCCTCAATTCCTATAAGTCAGCTCAGGATATTGCAACCTCTGAGCTGGCCCCAACTCATCCCATCCGTTTAGGATTGGCTTTGAACTTCTCTGTGTTTTACTACGAGATTCTAAATTCTCCTGACCGGGCTTGCAGTCTTGCCAAGCAGGCTTTTGATGAAGCCATTGCAGAGCTGGATACACTGGGAGAGGATTCATACAAGGATAGCACTTTGATAATGCAACTTCTTCGTGATAATCTCACATTGTGGACTTCGGATATGCAGGATGACGGGACAGATGAGATTAAACAAGCACCCAAGCCTCAAGCTGAGGCGCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

262

Amino Acids

29.35

Weight (kDa)

4.79

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 238 2.3e-103 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 2 cut(s) 21, 237
AciI CCGC 6 cut(s) 184, 235, 237, 309, 350, 450
AclI AACGTT 1 cut(s) 357
AclWI GGATC 1 cut(s) 290
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 1 cut(s) 135
AcyI GRCGYC 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 708
AfaI GTAC 2 cut(s) 35, 272
AgsI TTSAA 3 cut(s) 385, 424, 544
AjiI CACGTC 1 cut(s) 253
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
AluBI AGCT 7 cut(s) 46, 295, 340, 482, 505, 634, 770
AluI AGCT 7 cut(s) 46, 295, 340, 482, 505, 634, 770
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 3 cut(s) 106, 211, 426
AlwI GGATC 1 cut(s) 290
AoxI GGCC 2 cut(s) 447, 507
ApeKI GCWGC 2 cut(s) 5, 778
ApoI RAATTY 1 cut(s) 574
AspLEI GCGC 5 cut(s) 144, 182, 201, 441, 778
AspS9I GGNCC 1 cut(s) 508
AsuC2I CCSGG 1 cut(s) 587
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 3 cut(s) 129, 280, 297
BarI GAAGNNNNNNTAC 2 cut(s) 383, 415
BauI CACGAG 1 cut(s) 193
Bbv12I GWGCWC 1 cut(s) 297
BbvCI CCTCAGC 1 cut(s) 771
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 70, 221, 530
BceAI ACGGC 1 cut(s) 171
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciVI GTATCC 1 cut(s) 631
BcnI CCSGG 1 cut(s) 587
BcoDI GTCTC 3 cut(s) 106, 211, 426
BfoI RGCGCY 1 cut(s) 183
BfuI GTATCC 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 608
BisI GCNGC 6 cut(s) 6, 235, 238, 351, 450, 779
BlsI GCNGC 6 cut(s) 7, 236, 239, 352, 451, 780
Bme1390I CCNGG 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 2 cut(s) 181, 510
BmrFI CCNGG 1 cut(s) 587
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 1 cut(s) 210
BmuI ACTGGG 1 cut(s) 653
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 2 cut(s) 483, 771
BpuEI CTTGAG 2 cut(s) 305, 750
BpuMI CCSGG 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 235, 369
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 281, 311
Bse1I ACTGG 2 cut(s) 433, 648
Bse3DI GCAATG 1 cut(s) 624
BseDI CCNNGG 2 cut(s) 235, 369
BseGI GGATG 4 cut(s) 11, 517, 522, 733
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 5 cut(s) 408, 444, 492, 497, 762
BseNI ACTGG 2 cut(s) 433, 648
BseRI GAGGAG 5 cut(s) 86, 94, 140, 199, 242
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 2 cut(s) 21, 237
BshFI GGCC 2 cut(s) 449, 509
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 586
BslFI GGGAC 1 cut(s) 748
BsmAI GTCTC 3 cut(s) 106, 211, 426
BsmBI CGTCTC 3 cut(s) 106, 211, 426
BsmFI GGGAC 1 cut(s) 748
BsnI GGCC 2 cut(s) 449, 509
Bsp1286I GDGCHC 3 cut(s) 129, 280, 297
Bsp143I GATC 3 cut(s) 27, 174, 282
BspACI CCGC 6 cut(s) 184, 235, 237, 309, 350, 450
BspANI GGCC 2 cut(s) 449, 509
BspCNI CTCAG 5 cut(s) 409, 445, 493, 496, 763
BspFNI CGCG 2 cut(s) 21, 237
BspLI GGNNCC 2 cut(s) 181, 510
BspPI GGATC 1 cut(s) 290
BspQI GCTCTTC 2 cut(s) 117, 132
BspT107I GGYRCC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 624
BsrI ACTGG 2 cut(s) 433, 648
BssECI CCNNGG 2 cut(s) 235, 369
BssMI GATC 3 cut(s) 27, 174, 282
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 193
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 3 cut(s) 18, 117, 132
BstACI GRCGYC 1 cut(s) 180
BstC8I GCNNGC 6 cut(s) 48, 57, 184, 507, 592, 609
BstDEI CTNAG 5 cut(s) 417, 453, 483, 501, 771
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 4 cut(s) 11, 517, 522, 733
BstFNI CGCG 2 cut(s) 21, 237
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 5 cut(s) 144, 182, 201, 441, 778
BstKTI GATC 3 cut(s) 30, 177, 285
BstMAI GTCTC 3 cut(s) 106, 211, 426
BstMBI GATC 3 cut(s) 27, 174, 282
BstMWI GCNNNNNNNGC 7 cut(s) 47, 52, 56, 61, 188, 243, 608
BstSCI CCNGG 1 cut(s) 585
BstUI CGCG 2 cut(s) 21, 237
BstV1I GCAGC 1 cut(s) 17
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 2 cut(s) 449, 509
BtgI CCRYGG 1 cut(s) 235
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 4 cut(s) 11, 517, 522, 733
BtsIMutI CAGTG 1 cut(s) 641
Cac8I GCNNGC 6 cut(s) 48, 57, 184, 507, 592, 609
CfoI GCGC 5 cut(s) 144, 182, 201, 441, 778
Cfr13I GGNCC 1 cut(s) 508
Cfr42I CCGCGG 1 cut(s) 238
Csp6I GTAC 2 cut(s) 34, 271
CviAII CATG 1 cut(s) 88
CviQI GTAC 2 cut(s) 34, 271
DdeI CTNAG 5 cut(s) 417, 453, 483, 501, 771
DinI GGCGCC 1 cut(s) 181
DpnI GATC 3 cut(s) 29, 176, 284
DpnII GATC 3 cut(s) 27, 174, 282
DraIII CACNNNGTG 1 cut(s) 708
Eam1104I CTCTTC 3 cut(s) 18, 117, 132
EarI CTCTTC 3 cut(s) 18, 117, 132
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 3 cut(s) 129, 280, 297
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 135
EcoICRI GAGCTC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 3 cut(s) 129, 280, 297
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 3 cut(s) 106, 211, 426
FaeI CATG 1 cut(s) 91
FaiI YATR 5 cut(s) 89, 405, 474, 658, 722
FaqI GGGAC 1 cut(s) 748
FatI CATG 1 cut(s) 87
FauI CCCGC 1 cut(s) 191
Fnu4HI GCNGC 6 cut(s) 6, 235, 238, 351, 450, 779
FokI GGATG 3 cut(s) 504, 509, 740
FriOI GRGCYC 3 cut(s) 129, 280, 297
Fsp4HI GCNGC 6 cut(s) 6, 235, 238, 351, 450, 779
GlaI GCGC 5 cut(s) 143, 181, 200, 440, 777
GluI GCNGC 6 cut(s) 6, 235, 238, 351, 450, 779
GsuI CTGGAG 1 cut(s) 450
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 449, 509
HapII CCGG 1 cut(s) 586
HhaI GCGC 5 cut(s) 144, 182, 201, 441, 778
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 5 cut(s) 142, 180, 199, 439, 776
HinP1I GCGC 5 cut(s) 142, 180, 199, 439, 776
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 5 cut(s) 316, 332, 365, 568, 653
HpaII CCGG 1 cut(s) 586
Hpy166II GTNNAC 2 cut(s) 34, 711
Hpy188I TCNGA 3 cut(s) 282, 502, 718
Hpy188III TCNNGA 5 cut(s) 286, 329, 485, 581, 692
Hpy8I GTNNAC 2 cut(s) 34, 711
Hpy99I CGWCG 4 cut(s) 41, 137, 161, 257
HpyAV CCTTC 4 cut(s) 88, 110, 217, 385
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 4 cut(s) 36, 171, 252, 357
HpyCH4V TGCA 5 cut(s) 494, 594, 629, 682, 724
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 52, 56, 61, 188, 243, 608
HpyF3I CTNAG 5 cut(s) 417, 453, 483, 501, 771
HpySE526I ACGT 4 cut(s) 36, 171, 252, 357
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 5 cut(s) 142, 180, 199, 439, 776
KasI GGCGCC 1 cut(s) 179
KspI CCGCGG 1 cut(s) 238
Kzo9I GATC 3 cut(s) 27, 174, 282
LguI GCTCTTC 2 cut(s) 117, 132
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 210
MaeII ACGT 4 cut(s) 36, 171, 252, 357
MalI GATC 3 cut(s) 29, 176, 284
MboI GATC 3 cut(s) 27, 174, 282
MboII GAAGA 5 cut(s) 35, 134, 149, 397, 680
MhlI GDGCHC 3 cut(s) 129, 280, 297
MluCI AATT 2 cut(s) 466, 574
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 2 cut(s) 324, 340
MseI TTAA 1 cut(s) 747
MspA1I CMGCKG 2 cut(s) 237, 452
MspI CCGG 1 cut(s) 586
MspR9I CCNGG 1 cut(s) 587
MvnI CGCG 2 cut(s) 21, 237
MwoI GCNNNNNNNGC 7 cut(s) 47, 52, 56, 61, 188, 243, 608
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 1 cut(s) 587
NdeII GATC 3 cut(s) 27, 174, 282
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 2 cut(s) 181, 510
NmeAIII GCCGAG 2 cut(s) 76, 85
PciSI GCTCTTC 2 cut(s) 117, 132
PfeI GAWTC 4 cut(s) 316, 332, 568, 653
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 6 cut(s) 7, 236, 239, 352, 451, 780
PleI GAGTC 1 cut(s) 373
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 297
Psp1406I AACGTT 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 181, 510
PspPI GGNCC 1 cut(s) 508
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 2 cut(s) 35, 272
RsaNI GTAC 2 cut(s) 34, 271
SacI GAGCTC 1 cut(s) 297
SacII CCGCGG 1 cut(s) 238
SapI GCTCTTC 2 cut(s) 117, 132
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 6 cut(s) 6, 235, 238, 351, 450, 779
Sau3AI GATC 3 cut(s) 27, 174, 282
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 1 cut(s) 587
SduI GDGCHC 3 cut(s) 129, 280, 297
SfaNI GCATC 1 cut(s) 210
SfoI GGCGCC 1 cut(s) 181
Sfr303I CCGCGG 1 cut(s) 238
SgrBI CCGCGG 1 cut(s) 238
SmlI CTYRAG 2 cut(s) 320, 765
SmoI CTYRAG 2 cut(s) 320, 765
Sse9I AATT 2 cut(s) 466, 574
SsiI CCGC 6 cut(s) 184, 235, 237, 309, 350, 450
SspDI GGCGCC 1 cut(s) 179
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 585
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 4 cut(s) 39, 174, 255, 360
TaqI TCGA 4 cut(s) 135, 216, 285, 330
TasI AATT 2 cut(s) 466, 574
TatI WGTACW 1 cut(s) 270
TauI GCSGC 4 cut(s) 237, 240, 353, 452
TfiI GAWTC 4 cut(s) 316, 332, 568, 653
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TscAI CASTG 1 cut(s) 648
TseI GCWGC 2 cut(s) 5, 778
TspDTI ATGAA 4 cut(s) 76, 404, 633, 645
TspGWI ACGGA 2 cut(s) 145, 515
TspRI CASTG 1 cut(s) 648
Tth111I GACNNNGTC 1 cut(s) 251
XapI RAATTY 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.