MD10G1084500.v1.1

14-3-3-like protein 16R

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007005
Physical Location & Seq
Forward (+)
12408819 .. 12411711
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1084500.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGCGGCCTCATCCCCACGCGAAGAAAACGTCTACGTCGCCAAGCTCGCCGAGCAAGCGGAGCGCTACGAGGAGATGGTCGAGTTCATGGAGAAGGTCGTCTCCTCCCTCCCCGAAGGCGAGGAGCCCACCGTCGAAGAGCGCAACCTCCTCTCCGTCGCCTACAAGAACGTCATCGGCGCCCGCCGTGCCTCCTGGCGTATCGTCTCCTCCATCGAGCAGAAGGAGGAGAGCCGCGGCAACACCGACCACGTCGCCACCATCAAGGAGTACAGAGCTCGGATCGAGAACGAGCTCTCCAACATCTGCGGTGGGATCCTGAAGGTTCTCGAATCGAAGCTCGTTCCGTCTGCGAAAGTCGGAGAGTCCAAGGTGTTTTATCTGAAGATGAAAGGGGATTACCATAGGTATTTGGCTGAGTTCAAAACTGGGGATGAGCGTAAAGAGGCGGCTGAGAACACCCTCAATGCCTATAAGGCAGCGCAGGATATTGCAACCTCTGAGTTGGCTCCAACTCATCCCATCCGTTTAGGATTGGCTTTGAACTTCTCTGTGTTTTACTACGAGATTCTAAATTCTCCTGACCGGGCTTGCAGCCTTGCAAAGCAGGCTTTTGATGAAGCCATTGCAGAGCTGGATACACTGGGAGAGGATTCATACAAGGATAGCACTCTGATAATGCAACTTCTTCGTGATAATCTCACATTGTGGACTTCAGATATGCAGGATGACGGAACAGATGAGATTAAAGAAGCACCCAAGCCTCAGGCTGAGCCACAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

262

Amino Acids

29.39

Weight (kDa)

4.81

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 238 1.2e-103 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 33
AccII CGCG 2 cut(s) 21, 237
AciI CCGC 7 cut(s) 5, 59, 184, 235, 237, 309, 449
AclWI GGATC 3 cut(s) 290, 310, 323
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 3 cut(s) 341, 404, 699
AcyI GRCGYC 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 708
AfaI GTAC 1 cut(s) 272
AfeI AGCGCT 1 cut(s) 65
AgsI TTSAA 2 cut(s) 424, 544
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 194
AleI CACNNNNGTG 1 cut(s) 781
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
AluBI AGCT 5 cut(s) 46, 278, 295, 340, 634
AluI AGCT 5 cut(s) 46, 278, 295, 340, 634
Alw21I GWGCWC 2 cut(s) 280, 297
Alw26I GTCTC 2 cut(s) 106, 211
AlwI GGATC 3 cut(s) 290, 310, 323
Aor51HI AGCGCT 1 cut(s) 65
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 479, 594
ApoI RAATTY 1 cut(s) 574
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
AspLEI GCGC 4 cut(s) 66, 144, 182, 484
AsuC2I CCSGG 1 cut(s) 587
AxyI CCTNAGG 1 cut(s) 765
BamHI GGATCC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 3 cut(s) 129, 280, 297
Bbv12I GWGCWC 2 cut(s) 280, 297
BbvI GCAGC 2 cut(s) 491, 606
BccI CCATC 4 cut(s) 70, 221, 269, 530
BceAI ACGGC 1 cut(s) 171
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 1 cut(s) 196
BciVI GTATCC 1 cut(s) 631
BcnI CCSGG 1 cut(s) 587
BcoDI GTCTC 2 cut(s) 106, 211
BfoI RGCGCY 2 cut(s) 67, 183
BfuI GTATCC 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 476
BisI GCNGC 6 cut(s) 6, 235, 238, 450, 480, 595
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 6 cut(s) 7, 236, 239, 451, 481, 596
Bme1390I CCNGG 2 cut(s) 196, 587
BmgBI CACGTC 1 cut(s) 253
BmiI GGNNCC 4 cut(s) 126, 181, 317, 510
BmrFI CCNGG 2 cut(s) 196, 587
BmrI ACTGGG 2 cut(s) 438, 653
BmuI ACTGGG 2 cut(s) 438, 653
Bpu1102I GCTNAGC 1 cut(s) 771
BpuMI CCSGG 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 235, 369
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 281, 311
Bse1I ACTGG 2 cut(s) 433, 648
Bse21I CCTNAGG 1 cut(s) 765
Bse3DI GCAATG 1 cut(s) 624
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 235, 369
BseGI GGATG 5 cut(s) 11, 439, 517, 522, 733
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 5 cut(s) 408, 444, 492, 762, 779
BseNI ACTGG 2 cut(s) 433, 648
BseRI GAGGAG 6 cut(s) 86, 94, 137, 140, 199, 242
BseXI GCAGC 2 cut(s) 491, 606
Bsh1236I CGCG 2 cut(s) 21, 237
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 2 cut(s) 280, 297
BsiSI CCGG 1 cut(s) 586
BsmAI GTCTC 2 cut(s) 106, 211
BsmBI CGTCTC 2 cut(s) 106, 211
BsnI GGCC 1 cut(s) 8
Bsp1286I GDGCHC 3 cut(s) 129, 280, 297
Bsp143I GATC 2 cut(s) 282, 315
Bsp1720I GCTNAGC 1 cut(s) 771
BspACI CCGC 7 cut(s) 5, 59, 184, 235, 237, 309, 449
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 5 cut(s) 409, 445, 493, 763, 778
BspFNI CGCG 2 cut(s) 21, 237
BspLI GGNNCC 4 cut(s) 126, 181, 317, 510
BspPI GGATC 3 cut(s) 290, 310, 323
BspQI GCTCTTC 1 cut(s) 132
BspT107I GGYRCC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 624
BsrI ACTGG 2 cut(s) 433, 648
BssECI CCNNGG 2 cut(s) 235, 369
BssMI GATC 2 cut(s) 282, 315
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 180
BstC8I GCNNGC 5 cut(s) 48, 57, 184, 592, 609
BstDEI CTNAG 5 cut(s) 417, 453, 501, 765, 771
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 5 cut(s) 11, 439, 517, 522, 733
BstFNI CGCG 2 cut(s) 21, 237
BstH2I RGCGCY 2 cut(s) 67, 183
BstHHI GCGC 4 cut(s) 66, 144, 182, 484
BstKTI GATC 2 cut(s) 285, 318
BstMAI GTCTC 2 cut(s) 106, 211
BstMBI GATC 2 cut(s) 282, 315
BstMWI GCNNNNNNNGC 7 cut(s) 47, 52, 56, 61, 188, 476, 608
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 194, 585
BstUI CGCG 2 cut(s) 21, 237
BstV1I GCAGC 2 cut(s) 491, 606
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
Bsu36I CCTNAGG 1 cut(s) 765
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 235
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 5 cut(s) 11, 439, 517, 522, 733
BtsIMutI CAGTG 1 cut(s) 641
Cac8I GCNNGC 5 cut(s) 48, 57, 184, 592, 609
CfoI GCGC 4 cut(s) 66, 144, 182, 484
Cfr42I CCGCGG 1 cut(s) 238
Csp6I GTAC 1 cut(s) 271
CviAII CATG 1 cut(s) 88
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 5 cut(s) 417, 453, 501, 765, 771
DinI GGCGCC 1 cut(s) 181
DpnI GATC 2 cut(s) 284, 317
DpnII GATC 2 cut(s) 282, 315
DraIII CACNNNGTG 1 cut(s) 708
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Ecl136II GAGCTC 2 cut(s) 278, 295
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 3 cut(s) 129, 280, 297
Eco47III AGCGCT 1 cut(s) 65
Eco53kI GAGCTC 2 cut(s) 278, 295
Eco57I CTGAAG 3 cut(s) 341, 404, 699
Eco81I CCTNAGG 1 cut(s) 765
EcoICRI GAGCTC 2 cut(s) 278, 295
EcoRII CCWGG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 3 cut(s) 129, 280, 297
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 2 cut(s) 106, 211
FaeI CATG 1 cut(s) 91
FaiI YATR 5 cut(s) 89, 405, 474, 658, 722
FatI CATG 1 cut(s) 87
FauI CCCGC 1 cut(s) 191
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 6 cut(s) 6, 235, 238, 450, 480, 595
FokI GGATG 4 cut(s) 446, 504, 509, 740
FriOI GRGCYC 3 cut(s) 129, 280, 297
Fsp4HI GCNGC 6 cut(s) 6, 235, 238, 450, 480, 595
GlaI GCGC 4 cut(s) 65, 143, 181, 483
GluI GCNGC 6 cut(s) 6, 235, 238, 450, 480, 595
HaeII RGCGCY 2 cut(s) 67, 183
HaeIII GGCC 1 cut(s) 8
HapII CCGG 1 cut(s) 586
HhaI GCGC 4 cut(s) 66, 144, 182, 484
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 4 cut(s) 64, 142, 180, 482
HinP1I GCGC 4 cut(s) 64, 142, 180, 482
HinfI GANTC 4 cut(s) 332, 365, 568, 653
HpaII CCGG 1 cut(s) 586
Hpy166II GTNNAC 2 cut(s) 34, 711
Hpy188I TCNGA 6 cut(s) 282, 362, 384, 502, 675, 718
Hpy188III TCNNGA 5 cut(s) 286, 319, 329, 581, 692
Hpy8I GTNNAC 2 cut(s) 34, 711
Hpy99I CGWCG 4 cut(s) 41, 137, 161, 257
HpyAV CCTTC 4 cut(s) 88, 110, 217, 316
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 4 cut(s) 30, 36, 171, 252
HpyCH4V TGCA 6 cut(s) 494, 594, 602, 629, 682, 724
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 52, 56, 61, 188, 476, 608
HpyF3I CTNAG 5 cut(s) 417, 453, 501, 765, 771
HpySE526I ACGT 4 cut(s) 30, 36, 171, 252
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 4 cut(s) 64, 142, 180, 482
KasI GGCGCC 1 cut(s) 179
KspI CCGCGG 1 cut(s) 238
Kzo9I GATC 2 cut(s) 282, 315
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 3 cut(s) 61, 124, 514
Lsp1109I GCAGC 2 cut(s) 491, 606
MaeII ACGT 4 cut(s) 30, 36, 171, 252
MalI GATC 2 cut(s) 284, 317
MboI GATC 2 cut(s) 282, 315
MboII GAAGA 4 cut(s) 35, 149, 397, 680
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 3 cut(s) 129, 280, 297
MluCI AATT 1 cut(s) 574
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 3 cut(s) 324, 340, 536
MseI TTAA 1 cut(s) 747
MslI CAYNNNNRTG 1 cut(s) 781
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 1 cut(s) 586
MspR9I CCNGG 2 cut(s) 196, 587
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 2 cut(s) 21, 237
MwoI GCNNNNNNNGC 7 cut(s) 47, 52, 56, 61, 188, 476, 608
NarI GGCGCC 1 cut(s) 180
NciI CCSGG 1 cut(s) 587
NdeII GATC 2 cut(s) 282, 315
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 4 cut(s) 126, 181, 317, 510
NmeAIII GCCGAG 1 cut(s) 76
OliI CACNNNNGTG 1 cut(s) 781
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 3 cut(s) 332, 568, 653
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 6 cut(s) 7, 236, 239, 451, 481, 596
PleI GAGTC 1 cut(s) 373
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 373
Psp124BI GAGCTC 2 cut(s) 280, 297
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 4 cut(s) 126, 181, 317, 510
PsuI RGATCY 1 cut(s) 315
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 781
SacI GAGCTC 2 cut(s) 280, 297
SacII CCGCGG 1 cut(s) 238
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 6 cut(s) 6, 235, 238, 450, 480, 595
Sau3AI GATC 2 cut(s) 282, 315
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 2 cut(s) 196, 587
SduI GDGCHC 3 cut(s) 129, 280, 297
SfoI GGCGCC 1 cut(s) 181
Sfr303I CCGCGG 1 cut(s) 238
SgrBI CCGCGG 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 781
Sse9I AATT 1 cut(s) 574
SsiI CCGC 7 cut(s) 5, 59, 184, 235, 237, 309, 449
SspDI GGCGCC 1 cut(s) 179
SstI GAGCTC 2 cut(s) 280, 297
StyD4I CCNGG 2 cut(s) 194, 585
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 4 cut(s) 33, 39, 174, 255
TaqI TCGA 6 cut(s) 81, 135, 216, 285, 330, 335
TasI AATT 1 cut(s) 574
TatI WGTACW 1 cut(s) 270
TauI GCSGC 4 cut(s) 8, 237, 240, 452
TfiI GAWTC 3 cut(s) 332, 568, 653
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TscAI CASTG 1 cut(s) 648
TseI GCWGC 2 cut(s) 479, 594
TspDTI ATGAA 4 cut(s) 76, 404, 633, 645
TspGWI ACGGA 4 cut(s) 145, 336, 515, 747
TspRI CASTG 1 cut(s) 648
Tth111I GACNNNGTC 1 cut(s) 251
XapI RAATTY 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.