AT1G47670

amino acid transporter family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004402
Physical Location & Seq
Reverse (-)
17536625 .. 17539719
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G47670.1

Sequence Viewer

Length: 1560 bp
ATGGACGAAAGACCCGAGACAGAGCTAATATCAATACCAGCCACACCACGAGTTTCAACACCGGAGATTCTAACTCCCTCCGGTCAAAGATCACCTCGTCCGGCCACCAAACCATCGTCGGCGACATGGACTCCGACTTCGTTCATATCACCGAGGTTCTTGAGTCCGATTGGTACACCAATGAAACGAGTTCTTGTGAACATGAAAGGGTATCTTGAAGAAGTTGGTCATCTCACTAAGCTAAACCCACAAGATGCTTGGCTTCCAATCACTGAATCTCGTAATGGAAACGCTCATTACGCTGCTTTTCACAATCTCAACGCTGGTGTTGGTTTTCAAGCCCTTGTTCTTCCCGTCGCGTTTGCGTTTCTTGGCTGGAGTTGGGGAATACTGTCTTTAACAATAGCATATTGCTGGCAACTCTACACACTGTGGATTCTGGTTCAGTTGCACGAAGCTGTCCCCGGGAAACGCTACAATCGATATGTCGAGCTTGCACAAGCTGCTTTTGGAGAAAGGTTAGGAGTATGGCTTGCATTGTTTCCTACGGTTTACTTATCAGCAGGAACCGCGACAGCGCTGATTCTGATCGGTGGAGAGACAATGAAACTCTTCTTCCAGATAGTTTGCGGTCCATTATGCACCTCGAATCCGTTAACAACAGTTGAATGGTATTTGGTGTTTACATCTCTCTGCATCGTTCTGTCTCAGCTACCAAACCTCAATTCTATTGCGGGACTCTCCTTAATAGGAGCAGTGACTGCAATAACTTACTCCACAATGGTTTGGGTTCTCTCTGTGAGCCAACCAAGACCAGCCACTATCTCATACGAGCCTCTTTCTATGCCTTCGACTTCTGGTTCACTCTTCGCTGTTTTGAATGCTCTTGGCATTATAGCTTTTGCCTTCAGAGGTCACAACTTGGTTTTGGAAATTCAGTCGACGATGCCATCAACGTTTAAGCATCCTGCTCACGTACCAATGTGGAGAGGAGCCAAAATCTCTTACTTCCTCATTGCTCTGTGTATCTTTCCAATCTCCATCGGAGGCTTTTGGGCTTATGGGAACCTTATGCCTTCAGGAGGTATGCTTGCTGCTTTGTATGCATTCCACATTCACGATATCCCGAGAGGCTTATTAGCGACGGCATTTCTCCTAGTAGTCTTCAGCTGCTTGAGCAGTTTTCAGATATACTCCATGCCTGCCTTTGACAGCTTTGAAGCTGGCTACACAAGCAGAACCAACAAGCCGTGTTCGATTTGGGTCAGATCAGGTTTCCGAGTCTTTTTTGGTTTTGTCTCGTTTTTCATCGGTGTTGCTCTCCCTTTTCTGTCTAGTCTTGCGGGGTTGCTCGGTGGACTCACTCTCCCGGTCACATTTGCTTATCCTTGCTTCATGTGGGTCTTGATTAAGAAACCGGCGAAATACAGTTTCAATTGGTATTTCCATTGGGGATTAGGTTGGTTGGGAGTTGCATTCAGCTTGGCATTCTCCATAGGTGGGATCTGGAGTATGGTTACCAATGGACTTAAGCTCAAGTTCTTCAAGCCGCCTAACTAA

Protein Analysis

519

Amino Acids

57.12

Weight (kDa)

9.38

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 95 - 506 4.5e-57 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 941
AccII CGCG 2 cut(s) 359, 572
AciI CCGC 5 cut(s) 570, 630, 734, 1343, 1550
AclI AACGTT 1 cut(s) 956
AclWI GGATC 1 cut(s) 1511
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 933
AcuI CTGAAG 3 cut(s) 892, 1062, 1150
AdeI CACNNNGTG 1 cut(s) 432
AfaI GTAC 2 cut(s) 175, 978
AfeI AGCGCT 1 cut(s) 579
AfiI CCNNNNNNNGG 2 cut(s) 1082, 1500
AflII CTTAAG 1 cut(s) 1529
AgsI TTSAA 8 cut(s) 57, 218, 338, 668, 880, 1220, 1435, 1546
AjuI GAANNNNNNNTTGG 2 cut(s) 709, 741
Alw26I GTCTC 4 cut(s) 11, 593, 711, 1303
AlwI GGATC 1 cut(s) 1511
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 3 cut(s) 14, 464, 1126
Aor51HI AGCGCT 1 cut(s) 579
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 4 cut(s) 302, 503, 1094, 1170
ApoI RAATTY 1 cut(s) 933
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
Asp700I GAANNNNTTC 1 cut(s) 611
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 1 cut(s) 632
AsuC2I CCSGG 3 cut(s) 465, 466, 1370
AsuHPI GGTGA 2 cut(s) 84, 141
AvaI CYCGRG 3 cut(s) 14, 464, 1126
AvaII GGWCC 1 cut(s) 632
BauI CACGAG 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 1156
BbvI GCAGC 4 cut(s) 289, 490, 1081, 1157
BccI CCATC 3 cut(s) 121, 958, 1049
BceAI ACGGC 2 cut(s) 1161, 1234
BcnI CCSGG 3 cut(s) 465, 466, 1370
BcoDI GTCTC 4 cut(s) 11, 593, 711, 1303
BfaI CTAG 2 cut(s) 1157, 1335
BfoI RGCGCY 1 cut(s) 581
BfrI CTTAAG 1 cut(s) 1529
BisI GCNGC 5 cut(s) 303, 504, 1095, 1171, 1550
BlsI GCNGC 5 cut(s) 304, 505, 1096, 1172, 1551
Bme1390I CCNGG 3 cut(s) 465, 466, 1370
Bme18I GGWCC 1 cut(s) 632
BmeT110I CYCGRG 3 cut(s) 14, 464, 1126
BmgT120I GGNCC 1 cut(s) 632
BmiI GGNNCC 3 cut(s) 568, 994, 1067
BmrFI CCNGG 3 cut(s) 465, 466, 1370
BmsI GCATC 4 cut(s) 244, 705, 936, 973
BpiI GAAGAC 1 cut(s) 1156
BpmI CTGGAG 2 cut(s) 397, 1528
BpuEI CTTGAG 3 cut(s) 181, 1195, 1520
BpuMI CCSGG 3 cut(s) 465, 466, 1370
Bsa29I ATCGAT 1 cut(s) 481
BsaAI YACGTR 1 cut(s) 976
BsaBI GATNNNNATC 1 cut(s) 587
BsaJI CCNNGG 3 cut(s) 152, 463, 464
BsaWI WCCGGW 2 cut(s) 61, 80
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 1350, 1380
Bsc4I CCNNNNNNNGG 2 cut(s) 1082, 1500
Bse118I RCCGGY 1 cut(s) 1417
Bse3DI GCAATG 1 cut(s) 1014
Bse8I GATNNNNATC 1 cut(s) 587
BseCI ATCGAT 1 cut(s) 481
BseDI CCNNGG 3 cut(s) 152, 463, 464
BseGI GGATG 1 cut(s) 964
BseJI GATNNNNATC 1 cut(s) 587
BseLI CCNNNNNNNGG 2 cut(s) 1082, 1500
BseMI GCAATG 1 cut(s) 1014
BseMII CTCAG 1 cut(s) 722
BseRI GAGGAG 1 cut(s) 1005
BseXI GCAGC 4 cut(s) 289, 490, 1081, 1157
Bsh1236I CGCG 2 cut(s) 359, 572
BshFI GGCC 1 cut(s) 104
BshVI ATCGAT 1 cut(s) 481
BsiHKCI CYCGRG 3 cut(s) 14, 464, 1126
BsiSI CCGG 6 cut(s) 62, 81, 101, 465, 1370, 1418
BslFI GGGAC 2 cut(s) 446, 750
BslI CCNNNNNNNGG 2 cut(s) 1082, 1500
BsmAI GTCTC 4 cut(s) 11, 593, 711, 1303
BsmFI GGGAC 2 cut(s) 446, 750
BsmI GAATGC 4 cut(s) 886, 1106, 1475, 1487
BsnI GGCC 1 cut(s) 104
BsoBI CYCGRG 3 cut(s) 14, 464, 1126
Bsp143I GATC 4 cut(s) 89, 588, 1268, 1503
BspACI CCGC 5 cut(s) 570, 630, 734, 1343, 1550
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 1 cut(s) 721
BspDI ATCGAT 1 cut(s) 481
BspFNI CGCG 2 cut(s) 359, 572
BspLI GGNNCC 3 cut(s) 568, 994, 1067
BspPI GGATC 1 cut(s) 1511
BspTI CTTAAG 1 cut(s) 1529
BsrDI GCAATG 1 cut(s) 1014
BsrFI RCCGGY 1 cut(s) 1417
BssAI RCCGGY 1 cut(s) 1417
BssECI CCNNGG 3 cut(s) 152, 463, 464
BssMI GATC 4 cut(s) 89, 588, 1268, 1503
BssSI CACGAG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 48
Bst4CI ACNGT 5 cut(s) 393, 432, 550, 664, 1430
Bst6I CTCTTC 2 cut(s) 617, 872
BstAFI CTTAAG 1 cut(s) 1529
BstAPI GCANNNNNTGC 2 cut(s) 503, 761
BstBAI YACGTR 1 cut(s) 976
BstC8I GCNNGC 6 cut(s) 416, 495, 534, 1092, 1203, 1225
BstDEI CTNAG 2 cut(s) 237, 708
BstEII GGTNACC 1 cut(s) 1516
BstENI CCTNNNNNAGG 1 cut(s) 1080
BstF5I GGATG 1 cut(s) 964
BstFNI CGCG 2 cut(s) 359, 572
BstH2I RGCGCY 1 cut(s) 581
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 4 cut(s) 92, 591, 1271, 1506
BstMAI GTCTC 4 cut(s) 11, 593, 711, 1303
BstMBI GATC 4 cut(s) 89, 588, 1268, 1503
BstMWI GCNNNNNNNGC 7 cut(s) 299, 503, 569, 761, 1103, 1176, 1233
BstPI GGTNACC 1 cut(s) 1516
BstSCI CCNGG 3 cut(s) 463, 464, 1368
BstUI CGCG 2 cut(s) 359, 572
BstV1I GCAGC 4 cut(s) 289, 490, 1081, 1157
BstV2I GAAGAC 1 cut(s) 1156
BstX2I RGATCY 1 cut(s) 1503
BstYI RGATCY 1 cut(s) 1503
Bsu15I ATCGAT 1 cut(s) 481
BsuRI GGCC 1 cut(s) 104
BsuTUI ATCGAT 1 cut(s) 481
BtsCI GGATG 1 cut(s) 964
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 270, 428, 762
Cac8I GCNNGC 6 cut(s) 416, 495, 534, 1092, 1203, 1225
CaiI CAGNNNCTG 1 cut(s) 761
CfoI GCGC 1 cut(s) 580
Cfr10I RCCGGY 1 cut(s) 1417
Cfr13I GGNCC 1 cut(s) 632
Cfr9I CCCGGG 1 cut(s) 464
ClaI ATCGAT 1 cut(s) 481
Csp6I GTAC 2 cut(s) 174, 977
CviAII CATG 4 cut(s) 126, 202, 1198, 1396
CviQI GTAC 2 cut(s) 174, 977
DdeI CTNAG 2 cut(s) 237, 708
DpnI GATC 4 cut(s) 91, 590, 1270, 1505
DpnII GATC 4 cut(s) 89, 588, 1268, 1503
DraIII CACNNNGTG 1 cut(s) 432
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 2 cut(s) 617, 872
EarI CTCTTC 2 cut(s) 617, 872
Eco32I GATATC 1 cut(s) 1123
Eco47I GGWCC 1 cut(s) 632
Eco47III AGCGCT 1 cut(s) 579
Eco57I CTGAAG 3 cut(s) 892, 1062, 1150
Eco88I CYCGRG 3 cut(s) 14, 464, 1126
Eco91I GGTNACC 1 cut(s) 1516
EcoNI CCTNNNNNAGG 1 cut(s) 1080
EcoO65I GGTNACC 1 cut(s) 1516
EcoRV GATATC 1 cut(s) 1123
EcoT22I ATGCAT 1 cut(s) 1108
FaeI CATG 4 cut(s) 129, 205, 1201, 1399
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 2 cut(s) 446, 750
FatI CATG 4 cut(s) 125, 201, 1197, 1395
FauI CCCGC 2 cut(s) 727, 1336
FblI GTMKAC 1 cut(s) 941
Fnu4HI GCNGC 5 cut(s) 303, 504, 1095, 1171, 1550
FokI GGATG 1 cut(s) 951
Fsp4HI GCNGC 5 cut(s) 303, 504, 1095, 1171, 1550
FspBI CTAG 2 cut(s) 1157, 1335
GlaI GCGC 1 cut(s) 579
GluI GCNGC 5 cut(s) 303, 504, 1095, 1171, 1550
GsuI CTGGAG 2 cut(s) 397, 1528
HaeII RGCGCY 1 cut(s) 581
HaeIII GGCC 1 cut(s) 104
HapII CCGG 6 cut(s) 62, 81, 101, 465, 1370, 1418
HhaI GCGC 1 cut(s) 580
Hin1II CATG 4 cut(s) 129, 205, 1201, 1399
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HincII GTYRAC 2 cut(s) 657, 942
HindII GTYRAC 2 cut(s) 657, 942
HpaI GTTAAC 1 cut(s) 657
HpaII CCGG 6 cut(s) 62, 81, 101, 465, 1370, 1418
HphI GGTGA 2 cut(s) 84, 141
Hpy166II GTNNAC 8 cut(s) 176, 199, 553, 657, 684, 863, 942, 1358
Hpy188I TCNGA 8 cut(s) 135, 168, 588, 911, 1046, 1188, 1268, 1280
Hpy188III TCNNGA 8 cut(s) 160, 215, 619, 1080, 1118, 1126, 1405, 1507
Hpy8I GTNNAC 8 cut(s) 176, 199, 553, 657, 684, 863, 942, 1358
Hpy99I CGWCG 4 cut(s) 121, 359, 946, 1147
HpyAV CCTTC 3 cut(s) 858, 916, 1086
HpyCH4III ACNGT 5 cut(s) 393, 432, 550, 664, 1430
HpyCH4IV ACGT 2 cut(s) 956, 975
HpyCH4V TGCA 8 cut(s) 451, 497, 536, 642, 696, 764, 1106, 1475
HpyF10VI GCNNNNNNNGC 7 cut(s) 299, 503, 569, 761, 1103, 1176, 1233
HpyF3I CTNAG 2 cut(s) 237, 708
HpySE526I ACGT 2 cut(s) 956, 975
Hsp92II CATG 4 cut(s) 129, 205, 1201, 1399
HspAI GCGC 1 cut(s) 578
KspAI GTTAAC 1 cut(s) 657
Kzo9I GATC 4 cut(s) 89, 588, 1268, 1503
LmnI GCTCC 2 cut(s) 752, 992
Lsp1109I GCAGC 4 cut(s) 289, 490, 1081, 1157
LweI GCATC 4 cut(s) 244, 705, 936, 973
MaeI CTAG 2 cut(s) 1157, 1335
MaeII ACGT 2 cut(s) 956, 975
MaeIII GTNAC 4 cut(s) 757, 914, 1372, 1516
MalI GATC 4 cut(s) 91, 590, 1270, 1505
MboI GATC 4 cut(s) 89, 588, 1268, 1503
MboII GAAGA 7 cut(s) 230, 341, 604, 607, 859, 1156, 1534
MfeI CAATTG 1 cut(s) 1435
MflI RGATCY 1 cut(s) 1503
MluCI AATT 3 cut(s) 724, 933, 1435
MlyI GAGTC 5 cut(s) 124, 172, 732, 1290, 1353
MmeI TCCRAC 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 1108
MroXI GAANNNNTTC 1 cut(s) 611
MseI TTAA 6 cut(s) 398, 656, 746, 960, 1410, 1530
MspA1I CMGCKG 1 cut(s) 1170
MspCI CTTAAG 1 cut(s) 1529
MspI CCGG 6 cut(s) 62, 81, 101, 465, 1370, 1418
MspR9I CCNGG 3 cut(s) 465, 466, 1370
MunI CAATTG 1 cut(s) 1435
Mva1269I GAATGC 4 cut(s) 886, 1106, 1475, 1487
MvnI CGCG 2 cut(s) 359, 572
MwoI GCNNNNNNNGC 7 cut(s) 299, 503, 569, 761, 1103, 1176, 1233
NciI CCSGG 3 cut(s) 465, 466, 1370
NdeII GATC 4 cut(s) 89, 588, 1268, 1503
NlaIII CATG 4 cut(s) 129, 205, 1201, 1399
NlaIV GGNNCC 3 cut(s) 568, 994, 1067
NmuCI GTSAC 3 cut(s) 757, 914, 1372
NsiI ATGCAT 1 cut(s) 1108
PcsI WCGNNNNNNNCGW 2 cut(s) 12, 478
PctI GAATGC 4 cut(s) 886, 1106, 1475, 1487
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 5 cut(s) 67, 275, 436, 583, 649
PkrI GCNGC 5 cut(s) 304, 505, 1096, 1172, 1551
PleI GAGTC 5 cut(s) 124, 171, 732, 1289, 1353
PpsI GAGTC 5 cut(s) 124, 171, 732, 1289, 1353
Ppu21I YACGTR 1 cut(s) 976
Psp1406I AACGTT 1 cut(s) 956
PspEI GGTNACC 1 cut(s) 1516
PspN4I GGNNCC 3 cut(s) 568, 994, 1067
PspPI GGNCC 1 cut(s) 632
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 1 cut(s) 1503
PvuII CAGCTG 1 cut(s) 1170
RsaI GTAC 2 cut(s) 175, 978
RsaNI GTAC 2 cut(s) 174, 977
SalI GTCGAC 1 cut(s) 940
SaqAI TTAA 6 cut(s) 398, 656, 746, 960, 1410, 1530
SatI GCNGC 5 cut(s) 303, 504, 1095, 1171, 1550
Sau3AI GATC 4 cut(s) 89, 588, 1268, 1503
Sau96I GGNCC 1 cut(s) 632
SchI GAGTC 5 cut(s) 124, 172, 732, 1290, 1353
ScrFI CCNGG 3 cut(s) 465, 466, 1370
SfaNI GCATC 4 cut(s) 244, 705, 936, 973
SinI GGWCC 1 cut(s) 632
SmaI CCCGGG 1 cut(s) 466
SmlI CTYRAG 4 cut(s) 160, 1174, 1529, 1535
SmoI CTYRAG 4 cut(s) 160, 1174, 1529, 1535
Sse9I AATT 3 cut(s) 724, 933, 1435
SsiI CCGC 5 cut(s) 570, 630, 734, 1343, 1550
SspMI CTAG 2 cut(s) 1157, 1335
StyD4I CCNGG 3 cut(s) 463, 464, 1368
TaaI ACNGT 5 cut(s) 393, 432, 550, 664, 1430
TaiI ACGT 2 cut(s) 959, 978
TaqI TCGA 6 cut(s) 481, 489, 647, 851, 941, 1256
TasI AATT 3 cut(s) 724, 933, 1435
TauI GCSGC 1 cut(s) 1552
TfiI GAWTC 5 cut(s) 67, 275, 436, 583, 649
Tru1I TTAA 6 cut(s) 398, 656, 746, 960, 1410, 1530
Tru9I TTAA 6 cut(s) 398, 656, 746, 960, 1410, 1530
TscAI CASTG 3 cut(s) 277, 435, 762
TseFI GTSAC 3 cut(s) 757, 914, 1372
TseI GCWGC 4 cut(s) 302, 503, 1094, 1170
Tsp45I GTSAC 3 cut(s) 757, 914, 1372
TspDTI ATGAA 6 cut(s) 133, 197, 218, 620, 1297, 1384
TspGWI ACGGA 1 cut(s) 642
TspMI CCCGGG 1 cut(s) 464
TspRI CASTG 3 cut(s) 277, 435, 762
Vha464I CTTAAG 1 cut(s) 1529
VpaK11BI GGWCC 1 cut(s) 632
XagI CCTNNNNNAGG 1 cut(s) 1080
XapI RAATTY 1 cut(s) 933
XcmI CCANNNNNNNNNTGG 1 cut(s) 255
XmaI CCCGGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 611
XspI CTAG 2 cut(s) 1157, 1335
Zsp2I ATGCAT 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.