Rw6G038240

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004402
Physical Location & Seq
Reverse (-)
62425452 .. 62427898
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G038240.1

Sequence Viewer

Length: 1512 bp
ATGAGTAGTGAGGTGACGGAAGTGAAATCGAACTCAAAGACGGCAAGGAGGGCAGCAGATTCAGAAATCGAAGCTGCGGCAGCCGATATGTCAAGCCCAGCTGGTCCGCCAAAAACGCCTAAGAGCCCTTTTGCTTCGCGTTTTATGAGTACTCCTCTTGCCAGCCCCATGAAAAAGGCCATTGTAAGCATGCAAGGGTACTTGGAAGAAGTTGGACACTTTACCAAGCTCGATCCTCAAGACTCCTGGCTACCCATCACCGAGTCGAGAAATGGTAATGCCTACTACTCGGCTTTTCATACGCTCAGTTCCGGAATCGGAGTTCAAGCCCTCCTCCTTCCGCTGTCTTTCACTGTTCTCGGTTGGGCATGGGGAATCATATGCCTCTCAGTGGCTTTCATGTGGCAGTTGTATACTCTGTGGTTACTGATACAGCTCCATGAATCGCAATCTGGGATGCGCTACAGTCGATACCTCCGCCTTTCCATGGCCGCTTTTGGTGAGAAACTAGGGAAGTTGCTGGCGCTGTTTCCAATCATGTACCTCTCCGGTGGCACGTGTGTGACCCTGATCATGATTGGTGGTGGAACCATGAAGATTTTTTACCAAATCTGGTACTTGGTGTTCACCGGAATGGCCATTGTTCTTGCTCAGCTTCCTAACCTCAACTCCATTGCCGGGGTTTCGCTAATTGGAGCAATCACTGCTATAACTTACTGCACTCTCATTTGGATTGTTTCCATTACCAGAGATAGGCCAGCGGGTGTTAACTATGACTATCCAGTTCTTCCTCCCCAAGCAAAATCTGATCCAGCTGCTTCGGTTTTTAGTATTCTCAATGCGCTTGGGATCACTGTCTTTGCTTTTAGAGGCCACAATCTTGTGCTCGAAATACAGGGGACTATGCCTTCAAGCACAAAGAGCCCGTCGCGTTTGCCAATGTGGAAAGGGGTGAAGTTTGCATATCTCATCATCGCAATGTGCTTATTTCCCCTTGCAATTGGCGGCTACTGGGCATATGGAAATTTGATACCATCAAATGGAGGAATGTTAAATGCCTTGAACGAGTATCATGGACACGATACGTCAAAATTTGTGCTAGGTTTTACAAGCCTGCTGGTTGTGGTTAACTGCCTAACCTCGTTTCAAATATATGCAATGCCGGTGTTTGACAATCTAGAATTCAGATATACTAGCTGCATGAACAAACCTTGTCCGCGGTGGCTCCGCGCAGGGTTCAGGATTTTGTTTGGCTGCTTCGCCTGTTTTATTGCAGTGGCACTACCCTTCTTGCCAAGCTTGGCAGGGTTGATTGGAGGAGTTGCAGTACCAATCACATTAGCATATCCATGTTTCATGTGGGTACTTATAAAAAAACCTCCTAAACATATTGCAATTTTCAATTGGGTACTGGGATCCTTGGGGATAGTCCTTAGTATCTTGATTGTCACCGCAGCAATTTGGAATATAGCGACCATCGGAATAGAAGTTCACTTCTTCAAGCCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

503

Amino Acids

55.09

Weight (kDa)

9.17

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 91 - 489 7.3e-50 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1370
AccB7I CCANNNNNTGG 1 cut(s) 1040
AccI GTMKAC 1 cut(s) 413
AccII CGCG 4 cut(s) 139, 931, 1219, 1230
AccIII TCCGGA 1 cut(s) 311
AclWI GGATC 5 cut(s) 227, 803, 857, 1410, 1423
AcoI YGGCCR 2 cut(s) 489, 636
AcsI RAATTY 3 cut(s) 1024, 1091, 1181
AcvI CACGTG 1 cut(s) 558
AfaI GTAC 7 cut(s) 151, 200, 542, 617, 1329, 1365, 1410
AfiI CCNNNNNNNGG 5 cut(s) 391, 487, 678, 753, 1040
AflIII ACRYGT 1 cut(s) 557
AgsI TTSAA 6 cut(s) 326, 912, 1063, 1148, 1402, 1501
AjnI CCWGG 1 cut(s) 245
AleI CACNNNNGTG 1 cut(s) 560
AloI GAACNNNNNNTCC 4 cut(s) 306, 338, 1473, 1505
AluBI AGCT 8 cut(s) 74, 101, 229, 436, 655, 815, 1197, 1299
AluI AGCT 8 cut(s) 74, 101, 229, 436, 655, 815, 1197, 1299
Alw21I GWGCWC 1 cut(s) 888
AlwI GGATC 5 cut(s) 227, 803, 857, 1410, 1423
Aor13HI TCCGGA 1 cut(s) 311
AoxI GGCC 5 cut(s) 177, 489, 636, 755, 871
ApeKI GCWGC 7 cut(s) 53, 74, 80, 815, 1197, 1254, 1454
ApoI RAATTY 3 cut(s) 1024, 1091, 1181
ArsI GACNNNNNNTTYG 2 cut(s) 911, 943
AspLEI GCGC 4 cut(s) 462, 526, 844, 1232
AspS9I GGNCC 1 cut(s) 104
AsuC2I CCSGG 1 cut(s) 679
AsuHPI GGTGA 6 cut(s) 25, 250, 512, 619, 964, 1441
AvaII GGWCC 1 cut(s) 104
BalI TGGCCA 1 cut(s) 638
BamHI GGATCC 1 cut(s) 1415
BanII GRGCYC 2 cut(s) 128, 926
BarI GAAGNNNNNNTAC 2 cut(s) 587, 619
BbrPI CACGTG 1 cut(s) 558
Bbv12I GWGCWC 1 cut(s) 888
BbvI GCAGC 7 cut(s) 61, 65, 92, 802, 1184, 1241, 1466
BccI CCATC 3 cut(s) 263, 1042, 1484
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 247
BclI TGATCA 1 cut(s) 570
BcnI CCSGG 1 cut(s) 679
BfaI CTAG 4 cut(s) 509, 1100, 1178, 1194
BfmI CTRYAG 1 cut(s) 463
BfoI RGCGCY 1 cut(s) 527
BlpI GCTNAGC 1 cut(s) 651
BmcAI AGTACT 1 cut(s) 151
Bme1390I CCNGG 2 cut(s) 247, 679
Bme18I GGWCC 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 3 cut(s) 589, 1226, 1417
BmrFI CCNGG 2 cut(s) 247, 679
BmrI ACTGGG 2 cut(s) 1021, 1421
BmsI GCATC 1 cut(s) 447
BmuI ACTGGG 2 cut(s) 1021, 1421
BplI GAGNNNNNCTC 2 cut(s) 139, 171
Bpu1102I GCTNAGC 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 222
BpuMI CCSGG 1 cut(s) 679
BsaAI YACGTR 1 cut(s) 558
BsaJI CCNNGG 4 cut(s) 486, 678, 1217, 1419
BsaWI WCCGGW 3 cut(s) 311, 548, 629
BsaXI ACNNNNNCTCC 4 cut(s) 653, 683, 1311, 1341
Bsc4I CCNNNNNNNGG 5 cut(s) 391, 487, 678, 753, 1040
Bse118I RCCGGY 1 cut(s) 1162
Bse1I ACTGG 3 cut(s) 782, 1016, 1416
Bse3DI GCAATG 3 cut(s) 672, 984, 1164
BseAI TCCGGA 1 cut(s) 311
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 4 cut(s) 486, 678, 1217, 1419
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 5 cut(s) 391, 487, 678, 753, 1040
BseMI GCAATG 3 cut(s) 672, 984, 1164
BseMII CTCAG 3 cut(s) 319, 402, 665
BseNI ACTGG 3 cut(s) 782, 1016, 1416
BseRI GAGGAG 3 cut(s) 144, 323, 1332
BseXI GCAGC 7 cut(s) 61, 65, 92, 802, 1184, 1241, 1466
BseYI CCCAGC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 703
Bsh1236I CGCG 4 cut(s) 139, 931, 1219, 1230
BshFI GGCC 5 cut(s) 179, 491, 638, 757, 873
BsiHKAI GWGCWC 1 cut(s) 888
BsiSI CCGG 5 cut(s) 312, 549, 630, 678, 1163
BslFI GGGAC 1 cut(s) 913
BslI CCNNNNNNNGG 5 cut(s) 391, 487, 678, 753, 1040
BsmFI GGGAC 1 cut(s) 913
BsnI GGCC 5 cut(s) 179, 491, 638, 757, 873
Bsp1286I GDGCHC 3 cut(s) 128, 888, 926
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 5 cut(s) 232, 570, 808, 849, 1415
Bsp1720I GCTNAGC 1 cut(s) 651
Bsp19I CCATGG 1 cut(s) 486
BspANI GGCC 5 cut(s) 179, 491, 638, 757, 873
BspCNI CTCAG 3 cut(s) 318, 401, 664
BspEI TCCGGA 1 cut(s) 311
BspFNI CGCG 4 cut(s) 139, 931, 1219, 1230
BspHI TCATGA 1 cut(s) 573
BspLI GGNNCC 3 cut(s) 589, 1226, 1417
BspPI GGATC 5 cut(s) 227, 803, 857, 1410, 1423
BsrDI GCAATG 3 cut(s) 672, 984, 1164
BsrFI RCCGGY 1 cut(s) 1162
BsrI ACTGG 3 cut(s) 782, 1016, 1416
BssAI RCCGGY 1 cut(s) 1162
BssECI CCNNGG 4 cut(s) 486, 678, 1217, 1419
BssMI GATC 5 cut(s) 232, 570, 808, 849, 1415
BssNAI GTATAC 1 cut(s) 414
BssT1I CCWWGG 2 cut(s) 486, 1419
Bst1107I GTATAC 1 cut(s) 414
Bst2UI CCWGG 1 cut(s) 247
Bst4CI ACNGT 3 cut(s) 355, 467, 856
BstAPI GCANNNNNTGC 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 558
BstC8I GCNNGC 5 cut(s) 163, 191, 522, 759, 1115
BstDEI CTNAG 5 cut(s) 120, 305, 388, 651, 1433
BstDSI CCRYGG 2 cut(s) 486, 1217
BstF5I GGATG 1 cut(s) 462
BstFNI CGCG 4 cut(s) 139, 931, 1219, 1230
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 4 cut(s) 462, 526, 844, 1232
BstKTI GATC 5 cut(s) 235, 573, 811, 852, 1418
BstMBI GATC 5 cut(s) 232, 570, 808, 849, 1415
BstMWI GCNNNNNNNGC 5 cut(s) 50, 80, 115, 704, 921
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 245, 677
BstSFI CTRYAG 1 cut(s) 463
BstUI CGCG 4 cut(s) 139, 931, 1219, 1230
BstV1I GCAGC 7 cut(s) 61, 65, 92, 802, 1184, 1241, 1466
BstX2I RGATCY 1 cut(s) 1415
BstYI RGATCY 1 cut(s) 1415
BstZ17I GTATAC 1 cut(s) 414
BsuRI GGCC 5 cut(s) 179, 491, 638, 757, 873
BtgI CCRYGG 2 cut(s) 486, 1217
BtgZI GCGATG 1 cut(s) 958
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 2 cut(s) 702, 1281
BtsIMutI CAGTG 5 cut(s) 351, 396, 702, 852, 1281
Cac8I GCNNGC 5 cut(s) 163, 191, 522, 759, 1115
CciI TCATGA 1 cut(s) 573
CfoI GCGC 4 cut(s) 462, 526, 844, 1232
Cfr10I RCCGGY 1 cut(s) 1162
Cfr13I GGNCC 1 cut(s) 104
Cfr42I CCGCGG 1 cut(s) 1220
Csp6I GTAC 7 cut(s) 150, 199, 541, 616, 1328, 1364, 1409
CviQI GTAC 7 cut(s) 150, 199, 541, 616, 1328, 1364, 1409
DdeI CTNAG 5 cut(s) 120, 305, 388, 651, 1433
DpnI GATC 5 cut(s) 234, 572, 810, 851, 1417
DpnII GATC 5 cut(s) 232, 570, 808, 849, 1415
EaeI YGGCCR 2 cut(s) 489, 636
EciI GGCGGA 2 cut(s) 96, 467
Eco130I CCWWGG 2 cut(s) 486, 1419
Eco24I GRGCYC 2 cut(s) 128, 926
Eco47I GGWCC 1 cut(s) 104
Eco72I CACGTG 1 cut(s) 558
EcoRI GAATTC 1 cut(s) 1181
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 486, 1419
EcoT38I GRGCYC 2 cut(s) 128, 926
ErhI CCWWGG 2 cut(s) 486, 1419
FaqI GGGAC 1 cut(s) 913
FauI CCCGC 1 cut(s) 754
FauNDI CATATG 2 cut(s) 380, 1018
FbaI TGATCA 1 cut(s) 570
FblI GTMKAC 1 cut(s) 413
FokI GGATG 1 cut(s) 469
FriOI GRGCYC 2 cut(s) 128, 926
FspBI CTAG 4 cut(s) 509, 1100, 1178, 1194
GlaI GCGC 4 cut(s) 461, 525, 843, 1231
GsaI CCCAGC 1 cut(s) 101
HaeII RGCGCY 1 cut(s) 527
HaeIII GGCC 5 cut(s) 179, 491, 638, 757, 873
HapII CCGG 5 cut(s) 312, 549, 630, 678, 1163
HhaI GCGC 4 cut(s) 462, 526, 844, 1232
Hin6I GCGC 4 cut(s) 460, 524, 842, 1230
HinP1I GCGC 4 cut(s) 460, 524, 842, 1230
HincII GTYRAC 2 cut(s) 769, 1129
HindII GTYRAC 2 cut(s) 769, 1129
HindIII AAGCTT 1 cut(s) 1297
HinfI GANTC 6 cut(s) 59, 242, 263, 315, 375, 443
HpaI GTTAAC 2 cut(s) 769, 1129
HpaII CCGG 5 cut(s) 312, 549, 630, 678, 1163
HphI GGTGA 6 cut(s) 25, 250, 512, 619, 964, 1441
Hpy166II GTNNAC 5 cut(s) 414, 627, 769, 1129, 1492
Hpy188I TCNGA 5 cut(s) 64, 320, 808, 1187, 1481
Hpy188III TCNNGA 7 cut(s) 239, 267, 312, 574, 1178, 1240, 1441
Hpy8I GTNNAC 5 cut(s) 414, 627, 769, 1129, 1492
Hpy99I CGWCG 1 cut(s) 931
HpyAV CCTTC 3 cut(s) 347, 918, 1297
HpyCH4III ACNGT 3 cut(s) 355, 467, 856
HpyCH4IV ACGT 2 cut(s) 557, 1085
HpyCH4V TGCA 9 cut(s) 193, 720, 962, 998, 1157, 1200, 1274, 1325, 1394
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 80, 115, 704, 921
HpyF3I CTNAG 5 cut(s) 120, 305, 388, 651, 1433
HpySE526I ACGT 2 cut(s) 557, 1085
HspAI GCGC 4 cut(s) 460, 524, 842, 1230
Kpn2I TCCGGA 1 cut(s) 311
Ksp22I TGATCA 1 cut(s) 570
KspAI GTTAAC 2 cut(s) 769, 1129
KspI CCGCGG 1 cut(s) 1220
Kzo9I GATC 5 cut(s) 232, 570, 808, 849, 1415
LmnI GCTCC 3 cut(s) 441, 695, 1230
Lsp1109I GCAGC 7 cut(s) 61, 65, 92, 802, 1184, 1241, 1466
LweI GCATC 1 cut(s) 447
MaeI CTAG 4 cut(s) 509, 1100, 1178, 1194
MaeII ACGT 2 cut(s) 557, 1085
MaeIII GTNAC 4 cut(s) 13, 423, 562, 1447
MalI GATC 5 cut(s) 234, 572, 810, 851, 1417
MboI GATC 5 cut(s) 232, 570, 808, 849, 1415
MboII GAAGA 4 cut(s) 218, 607, 779, 1489
MfeI CAATTG 2 cut(s) 999, 1402
MflI RGATCY 1 cut(s) 1415
MhlI GDGCHC 3 cut(s) 128, 888, 926
MlsI TGGCCA 1 cut(s) 638
MluCI AATT 8 cut(s) 690, 999, 1024, 1091, 1181, 1395, 1402, 1458
MluNI TGGCCA 1 cut(s) 638
MlyI GAGTC 2 cut(s) 236, 272
MmeI TCCRAC 1 cut(s) 193
Mox20I TGGCCA 1 cut(s) 638
MroI TCCGGA 1 cut(s) 311
MscI TGGCCA 1 cut(s) 638
MseI TTAA 3 cut(s) 768, 1052, 1128
MslI CAYNNNNRTG 4 cut(s) 560, 632, 977, 1348
Msp20I TGGCCA 1 cut(s) 638
MspA1I CMGCKG 5 cut(s) 101, 343, 761, 815, 1219
MspI CCGG 5 cut(s) 312, 549, 630, 678, 1163
MspR9I CCNGG 2 cut(s) 247, 679
MunI CAATTG 2 cut(s) 999, 1402
MvaI CCWGG 1 cut(s) 247
MvnI CGCG 4 cut(s) 139, 931, 1219, 1230
MwoI GCNNNNNNNGC 5 cut(s) 50, 80, 115, 704, 921
NciI CCSGG 1 cut(s) 679
NcoI CCATGG 1 cut(s) 486
NdeI CATATG 2 cut(s) 380, 1018
NdeII GATC 5 cut(s) 232, 570, 808, 849, 1415
NlaIV GGNNCC 3 cut(s) 589, 1226, 1417
NmeAIII GCCGAG 1 cut(s) 269
NmuCI GTSAC 3 cut(s) 13, 562, 1447
NspI RCATGY 1 cut(s) 193
OliI CACNNNNGTG 1 cut(s) 560
PaeI GCATGC 1 cut(s) 193
PagI TCATGA 1 cut(s) 573
PfeI GAWTC 4 cut(s) 59, 315, 375, 443
PflMI CCANNNNNTGG 1 cut(s) 1040
PleI GAGTC 2 cut(s) 236, 271
PmaCI CACGTG 1 cut(s) 558
PmlI CACGTG 1 cut(s) 558
PpsI GAGTC 2 cut(s) 236, 271
Ppu21I YACGTR 1 cut(s) 558
PsiI TTATAA 1 cut(s) 1370
Psp6I CCWGG 1 cut(s) 245
PspCI CACGTG 1 cut(s) 558
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 3 cut(s) 589, 1226, 1417
PspPI GGNCC 1 cut(s) 104
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
PsuI RGATCY 1 cut(s) 1415
PvuII CAGCTG 2 cut(s) 101, 815
RsaI GTAC 7 cut(s) 151, 200, 542, 617, 1329, 1365, 1410
RsaNI GTAC 7 cut(s) 150, 199, 541, 616, 1328, 1364, 1409
RseI CAYNNNNRTG 4 cut(s) 560, 632, 977, 1348
SacII CCGCGG 1 cut(s) 1220
SaqAI TTAA 3 cut(s) 768, 1052, 1128
Sau3AI GATC 5 cut(s) 232, 570, 808, 849, 1415
Sau96I GGNCC 1 cut(s) 104
ScaI AGTACT 1 cut(s) 151
SchI GAGTC 2 cut(s) 236, 272
ScrFI CCNGG 2 cut(s) 247, 679
SduI GDGCHC 3 cut(s) 128, 888, 926
SfaNI GCATC 1 cut(s) 447
SfcI CTRYAG 1 cut(s) 463
Sfr303I CCGCGG 1 cut(s) 1220
SgrBI CCGCGG 1 cut(s) 1220
SinI GGWCC 1 cut(s) 104
SmiMI CAYNNNNRTG 4 cut(s) 560, 632, 977, 1348
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SphI GCATGC 1 cut(s) 193
Sse9I AATT 8 cut(s) 690, 999, 1024, 1091, 1181, 1395, 1402, 1458
SspMI CTAG 4 cut(s) 509, 1100, 1178, 1194
StyD4I CCNGG 2 cut(s) 245, 677
StyI CCWWGG 2 cut(s) 486, 1419
TaaI ACNGT 3 cut(s) 355, 467, 856
TaiI ACGT 2 cut(s) 560, 1088
TaqI TCGA 6 cut(s) 29, 69, 231, 266, 469, 888
TasI AATT 8 cut(s) 690, 999, 1024, 1091, 1181, 1395, 1402, 1458
TatI WGTACW 1 cut(s) 149
TauI GCSGC 3 cut(s) 80, 494, 1008
TfiI GAWTC 4 cut(s) 59, 315, 375, 443
Tru1I TTAA 3 cut(s) 768, 1052, 1128
Tru9I TTAA 3 cut(s) 768, 1052, 1128
TscAI CASTG 5 cut(s) 358, 396, 709, 859, 1281
TseFI GTSAC 3 cut(s) 13, 562, 1447
TseI GCWGC 7 cut(s) 53, 74, 80, 815, 1197, 1254, 1454
Tsp45I GTSAC 3 cut(s) 13, 562, 1447
TspDTI ATGAA 7 cut(s) 185, 287, 388, 456, 608, 1217, 1345
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 5 cut(s) 358, 396, 709, 859, 1281
Van91I CCANNNNNTGG 1 cut(s) 1040
VpaK11BI GGWCC 1 cut(s) 104
XapI RAATTY 3 cut(s) 1024, 1091, 1181
XbaI TCTAGA 1 cut(s) 1177
XceI RCATGY 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 1356
XmiI GTMKAC 1 cut(s) 413
XspI CTAG 4 cut(s) 509, 1100, 1178, 1194
ZrmI AGTACT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.