Rroxscaffold_7G00164130

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004402
Physical Location & Seq
Forward (+)
6191774 .. 6195246
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164130.1

Sequence Viewer

Length: 1383 bp
ATGAAAAAGGCCATTGCAAGCATGCAAGGCTACTTGGAAGAAGTTGGACACTTCACCAAGCTCGATCCTCAAGACGCCTGGCTACCCATCACCGAGTCGAGAAATGGTAATGCCTACTACTCGGCGTTTCACACACTCAGTTCCGGAATCGGAGTTCAAGCCCTCCTCCTTCCGCTGTCTTTCACTGTTCTCGGTTGGGCATGGGGAATCATATGCCTCTCAGTGGCTTTCATGTGGCAGTTGTATACTCTGTGGTTACTGATACAGCTCCATGAATCGCAATCTGGGATGCGCTACAGTCGATACCTCCGCCTTTCCATGGCCGCTTTTGGTGAGAAACTAGGGAAGTTGCTGGCGCTGTTTCCAATCATGTACCTCTCCGGTGGCACGTGTGTGACCCTAATCATGATTGGTGGTGGAACCATGAAGATTTTTTACCAAATTGTTAGCGGTAGTAATACAAGTCCTTTGACCATCCTGGAGTGGTACTTGGTGTTCACCGGCATGGCCATTGTTCTTGCTCAGCTTCCTAACCTCAACTCCATAGCCGGGGTTTCGCTAATCGGAGCAATCACTGCTATAACTTACTGCACTCTCATTTGGATTGTTTCCATTACCAGAGATAGGCCAGCGGGTGTTAACTATGACTATCCAGTTCTTCCTCCCCAAGCAAAATCTGATCCAGCTGCTACGGTTTTTAGTATTCTCAATGCGCTTGGGATCACTGTCTTTGCTTTTAGAGGCCACAATCTTGTGCTCGAAATACAGGGGACTATGCCTTCAAGCACAAAGAGCCCCTCGCGTTTGCCAATGTGGAAAGGGGTGAAGTTTGCATATCTCATCATCGGAATGTGCTTGTTTCCCCTTGCAATTGGCGGCTACTGGGCTTATGGAAATTTGATACCATCAAACGGAGGAATGTTAAATGCCTTGAACGAGTATCATGGACACGATACGTCAAAATTTGTGCTAGGTTTTACGAGCCTGCTGGTTGTGGTTAACTGCCTAACCTCGTTTCAAATATATGCAATGCCGGTGTTTGACAATCTGGAATTCAGATATACTAGCTGCATGAACAAACCCTGTCCACGGTGGCTCCGCGCAGGGTTCAGGATTTTGTTTGGCTGCTTCGCCTGTTTTATTGCAGTGGCACTACCCTTCTTGCCAAGCTTGGCAGGGTTGATTGGAGGAGTTGCAGTACCAATCACATTAGCATATCCATGTTTCATGTGGGTACTTATAAAAAACCCTCCTAAACATATCGCAATTTTCAATTGGGTACTCGGATCCTTAGGGATAGTCCTTAGTATCTTGATTGTCACCGGAGCAATTTGGAATATAGTGACCATCGGAATAGAAGTTCACTTCTTCAAGCCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

460

Amino Acids

50.58

Weight (kDa)

8.99

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 35 - 447 1.9e-52 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1241
AccI GTMKAC 1 cut(s) 245
AccII CGCG 2 cut(s) 802, 1101
AccIII TCCGGA 1 cut(s) 143
AciI CCGC 7 cut(s) 173, 310, 324, 450, 632, 876, 1099
AclWI GGATC 5 cut(s) 59, 674, 728, 1281, 1294
AcoI YGGCCR 2 cut(s) 321, 507
AcsI RAATTY 3 cut(s) 895, 962, 1052
AcvI CACGTG 1 cut(s) 390
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 5 cut(s) 374, 488, 1200, 1236, 1281
AfiI CCNNNNNNNGG 6 cut(s) 223, 319, 549, 624, 911, 1089
AflIII ACRYGT 1 cut(s) 389
AgsI TTSAA 6 cut(s) 158, 783, 934, 1019, 1273, 1372
AjnI CCWGG 2 cut(s) 77, 477
AleI CACNNNNGTG 1 cut(s) 392
AloI GAACNNNNNNTCC 4 cut(s) 138, 170, 1344, 1376
AluBI AGCT 6 cut(s) 61, 268, 526, 686, 1068, 1170
AluI AGCT 6 cut(s) 61, 268, 526, 686, 1068, 1170
Alw21I GWGCWC 1 cut(s) 759
AlwI GGATC 5 cut(s) 59, 674, 728, 1281, 1294
Aor13HI TCCGGA 1 cut(s) 143
AoxI GGCC 5 cut(s) 9, 321, 507, 626, 742
ApeKI GCWGC 3 cut(s) 686, 1068, 1125
ApoI RAATTY 3 cut(s) 895, 962, 1052
AspLEI GCGC 4 cut(s) 294, 358, 715, 1103
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 6 cut(s) 46, 82, 344, 490, 835, 1312
AxyI CCTNAGG 1 cut(s) 1291
BalI TGGCCA 1 cut(s) 509
BamHI GGATCC 1 cut(s) 1286
BanII GRGCYC 1 cut(s) 797
BarI GAAGNNNNNNTAC 2 cut(s) 419, 451
BbrPI CACGTG 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 759
BbvI GCAGC 3 cut(s) 673, 1055, 1112
BccI CCATC 4 cut(s) 95, 482, 913, 1355
BciT130I CCWGG 2 cut(s) 79, 479
BcnI CCSGG 1 cut(s) 550
BfaI CTAG 3 cut(s) 341, 971, 1065
BfmI CTRYAG 1 cut(s) 295
BfoI RGCGCY 1 cut(s) 359
BisI GCNGC 5 cut(s) 324, 687, 877, 1069, 1126
BlpI GCTNAGC 1 cut(s) 522
BlsI GCNGC 5 cut(s) 325, 688, 878, 1070, 1127
Bme1390I CCNGG 3 cut(s) 79, 479, 550
BmiI GGNNCC 3 cut(s) 421, 1097, 1288
BmrFI CCNGG 3 cut(s) 79, 479, 550
BmrI ACTGGG 1 cut(s) 892
BmsI GCATC 1 cut(s) 279
BmuI ACTGGG 1 cut(s) 892
BpmI CTGGAG 1 cut(s) 500
Bpu1102I GCTNAGC 1 cut(s) 522
BpuEI CTTGAG 1 cut(s) 54
BpuMI CCSGG 1 cut(s) 550
BsaAI YACGTR 1 cut(s) 390
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 318, 549, 1088
BsaWI WCCGGW 3 cut(s) 143, 380, 1322
BsaXI ACNNNNNCTCC 6 cut(s) 524, 554, 1080, 1110, 1182, 1212
Bsc4I CCNNNNNNNGG 6 cut(s) 223, 319, 549, 624, 911, 1089
Bse118I RCCGGY 2 cut(s) 500, 1033
Bse1I ACTGG 2 cut(s) 653, 887
Bse21I CCTNAGG 1 cut(s) 1291
Bse3DI GCAATG 2 cut(s) 12, 1035
BseAI TCCGGA 1 cut(s) 143
BseBI CCWGG 2 cut(s) 79, 479
BseDI CCNNGG 3 cut(s) 318, 549, 1088
BseGI GGATG 2 cut(s) 294, 474
BseLI CCNNNNNNNGG 6 cut(s) 223, 319, 549, 624, 911, 1089
BseMI GCAATG 2 cut(s) 12, 1035
BseMII CTCAG 3 cut(s) 151, 234, 536
BseNI ACTGG 2 cut(s) 653, 887
BseRI GAGGAG 2 cut(s) 155, 1203
BseXI GCAGC 3 cut(s) 673, 1055, 1112
BsgI GTGCAG 1 cut(s) 574
Bsh1236I CGCG 2 cut(s) 802, 1101
BshFI GGCC 5 cut(s) 11, 323, 509, 628, 744
BsiHKAI GWGCWC 1 cut(s) 759
BsiSI CCGG 6 cut(s) 144, 381, 501, 549, 1034, 1323
BslFI GGGAC 1 cut(s) 784
BslI CCNNNNNNNGG 6 cut(s) 223, 319, 549, 624, 911, 1089
BsmFI GGGAC 1 cut(s) 784
BsnI GGCC 5 cut(s) 11, 323, 509, 628, 744
Bsp1286I GDGCHC 2 cut(s) 759, 797
Bsp13I TCCGGA 1 cut(s) 143
Bsp143I GATC 4 cut(s) 64, 679, 720, 1286
Bsp1720I GCTNAGC 1 cut(s) 522
Bsp19I CCATGG 1 cut(s) 318
BspACI CCGC 7 cut(s) 173, 310, 324, 450, 632, 876, 1099
BspANI GGCC 5 cut(s) 11, 323, 509, 628, 744
BspCNI CTCAG 3 cut(s) 150, 233, 535
BspEI TCCGGA 1 cut(s) 143
BspFNI CGCG 2 cut(s) 802, 1101
BspHI TCATGA 1 cut(s) 405
BspLI GGNNCC 3 cut(s) 421, 1097, 1288
BspPI GGATC 5 cut(s) 59, 674, 728, 1281, 1294
BsrDI GCAATG 2 cut(s) 12, 1035
BsrFI RCCGGY 2 cut(s) 500, 1033
BsrI ACTGG 2 cut(s) 653, 887
BssAI RCCGGY 2 cut(s) 500, 1033
BssECI CCNNGG 3 cut(s) 318, 549, 1088
BssMI GATC 4 cut(s) 64, 679, 720, 1286
BssNAI GTATAC 1 cut(s) 246
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 318
Bst1107I GTATAC 1 cut(s) 246
Bst2UI CCWGG 2 cut(s) 79, 479
Bst4CI ACNGT 5 cut(s) 187, 299, 694, 727, 1092
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 575
BstBAI YACGTR 1 cut(s) 390
BstC8I GCNNGC 5 cut(s) 19, 23, 354, 630, 986
BstDEI CTNAG 5 cut(s) 137, 220, 522, 1291, 1304
BstDSI CCRYGG 2 cut(s) 318, 1088
BstF5I GGATG 2 cut(s) 294, 474
BstFNI CGCG 2 cut(s) 802, 1101
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 4 cut(s) 294, 358, 715, 1103
BstKTI GATC 4 cut(s) 67, 682, 723, 1289
BstMBI GATC 4 cut(s) 64, 679, 720, 1286
BstMWI GCNNNNNNNGC 3 cut(s) 27, 575, 792
BstNI CCWGG 2 cut(s) 79, 479
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 3 cut(s) 77, 477, 548
BstSFI CTRYAG 1 cut(s) 295
BstUI CGCG 2 cut(s) 802, 1101
BstV1I GCAGC 3 cut(s) 673, 1055, 1112
BstX2I RGATCY 1 cut(s) 1286
BstYI RGATCY 1 cut(s) 1286
BstZ17I GTATAC 1 cut(s) 246
Bsu36I CCTNAGG 1 cut(s) 1291
BsuRI GGCC 5 cut(s) 11, 323, 509, 628, 744
BtgI CCRYGG 2 cut(s) 318, 1088
BtsCI GGATG 2 cut(s) 294, 474
BtsI GCAGTG 2 cut(s) 573, 1152
BtsIMutI CAGTG 5 cut(s) 183, 228, 573, 723, 1152
Cac8I GCNNGC 5 cut(s) 19, 23, 354, 630, 986
CciI TCATGA 1 cut(s) 405
CfoI GCGC 4 cut(s) 294, 358, 715, 1103
Cfr10I RCCGGY 2 cut(s) 500, 1033
CseI GACGC 1 cut(s) 83
Csp6I GTAC 5 cut(s) 373, 487, 1199, 1235, 1280
CviQI GTAC 5 cut(s) 373, 487, 1199, 1235, 1280
DdeI CTNAG 5 cut(s) 137, 220, 522, 1291, 1304
DpnI GATC 4 cut(s) 66, 681, 722, 1288
DpnII GATC 4 cut(s) 64, 679, 720, 1286
EaeI YGGCCR 2 cut(s) 321, 507
EciI GGCGGA 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 797
Eco72I CACGTG 1 cut(s) 390
Eco81I CCTNAGG 1 cut(s) 1291
EcoRI GAATTC 1 cut(s) 1052
EcoRII CCWGG 2 cut(s) 77, 477
EcoT14I CCWWGG 1 cut(s) 318
EcoT38I GRGCYC 1 cut(s) 797
ErhI CCWWGG 1 cut(s) 318
FaqI GGGAC 1 cut(s) 784
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 212
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 5 cut(s) 324, 687, 877, 1069, 1126
FokI GGATG 2 cut(s) 301, 461
FriOI GRGCYC 1 cut(s) 797
Fsp4HI GCNGC 5 cut(s) 324, 687, 877, 1069, 1126
FspBI CTAG 3 cut(s) 341, 971, 1065
GlaI GCGC 4 cut(s) 293, 357, 714, 1102
GluI GCNGC 5 cut(s) 324, 687, 877, 1069, 1126
GsuI CTGGAG 1 cut(s) 500
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 5 cut(s) 11, 323, 509, 628, 744
HapII CCGG 6 cut(s) 144, 381, 501, 549, 1034, 1323
HgaI GACGC 1 cut(s) 83
HhaI GCGC 4 cut(s) 294, 358, 715, 1103
Hin1I GRCGYC 1 cut(s) 75
Hin6I GCGC 4 cut(s) 292, 356, 713, 1101
HinP1I GCGC 4 cut(s) 292, 356, 713, 1101
HincII GTYRAC 2 cut(s) 640, 1000
HindII GTYRAC 2 cut(s) 640, 1000
HindIII AAGCTT 1 cut(s) 1168
HinfI GANTC 4 cut(s) 95, 147, 207, 275
HpaI GTTAAC 2 cut(s) 640, 1000
HpaII CCGG 6 cut(s) 144, 381, 501, 549, 1034, 1323
HphI GGTGA 6 cut(s) 46, 82, 344, 490, 835, 1312
Hpy166II GTNNAC 6 cut(s) 246, 498, 640, 1000, 1088, 1363
Hpy188I TCNGA 7 cut(s) 152, 566, 679, 848, 1058, 1286, 1352
Hpy188III TCNNGA 7 cut(s) 71, 99, 144, 406, 1049, 1111, 1312
Hpy8I GTNNAC 6 cut(s) 246, 498, 640, 1000, 1088, 1363
HpyAV CCTTC 3 cut(s) 179, 789, 1168
HpyCH4III ACNGT 5 cut(s) 187, 299, 694, 727, 1092
HpyCH4IV ACGT 2 cut(s) 389, 956
HpyCH4V TGCA 9 cut(s) 17, 25, 591, 833, 869, 1028, 1071, 1145, 1196
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 575, 792
HpyF3I CTNAG 5 cut(s) 137, 220, 522, 1291, 1304
HpySE526I ACGT 2 cut(s) 389, 956
Hsp92I GRCGYC 1 cut(s) 75
HspAI GCGC 4 cut(s) 292, 356, 713, 1101
Kpn2I TCCGGA 1 cut(s) 143
KspAI GTTAAC 2 cut(s) 640, 1000
Kzo9I GATC 4 cut(s) 64, 679, 720, 1286
LmnI GCTCC 4 cut(s) 273, 566, 1101, 1325
Lsp1109I GCAGC 3 cut(s) 673, 1055, 1112
LweI GCATC 1 cut(s) 279
MaeI CTAG 3 cut(s) 341, 971, 1065
MaeII ACGT 2 cut(s) 389, 956
MaeIII GTNAC 4 cut(s) 255, 394, 1318, 1342
MalI GATC 4 cut(s) 66, 681, 722, 1288
MboI GATC 4 cut(s) 64, 679, 720, 1286
MboII GAAGA 4 cut(s) 50, 439, 650, 1360
MfeI CAATTG 2 cut(s) 870, 1273
MflI RGATCY 1 cut(s) 1286
MhlI GDGCHC 2 cut(s) 759, 797
MlsI TGGCCA 1 cut(s) 509
MluCI AATT 8 cut(s) 441, 870, 895, 962, 1052, 1266, 1273, 1329
MluNI TGGCCA 1 cut(s) 509
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 25
Mox20I TGGCCA 1 cut(s) 509
MroI TCCGGA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 509
MseI TTAA 3 cut(s) 639, 923, 999
MslI CAYNNNNRTG 4 cut(s) 392, 503, 848, 1219
Msp20I TGGCCA 1 cut(s) 509
MspA1I CMGCKG 3 cut(s) 175, 632, 686
MspI CCGG 6 cut(s) 144, 381, 501, 549, 1034, 1323
MspR9I CCNGG 3 cut(s) 79, 479, 550
MunI CAATTG 2 cut(s) 870, 1273
MvaI CCWGG 2 cut(s) 79, 479
MvnI CGCG 2 cut(s) 802, 1101
MwoI GCNNNNNNNGC 3 cut(s) 27, 575, 792
NciI CCSGG 1 cut(s) 550
NcoI CCATGG 1 cut(s) 318
NdeI CATATG 1 cut(s) 212
NdeII GATC 4 cut(s) 64, 679, 720, 1286
NlaIV GGNNCC 3 cut(s) 421, 1097, 1288
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 3 cut(s) 394, 1318, 1342
NspI RCATGY 1 cut(s) 25
OliI CACNNNNGTG 1 cut(s) 392
PaeI GCATGC 1 cut(s) 25
PagI TCATGA 1 cut(s) 405
PfeI GAWTC 3 cut(s) 147, 207, 275
PfoI TCCNGGA 1 cut(s) 477
PkrI GCNGC 5 cut(s) 325, 688, 878, 1070, 1127
PleI GAGTC 1 cut(s) 103
PmaCI CACGTG 1 cut(s) 390
PmlI CACGTG 1 cut(s) 390
PpsI GAGTC 1 cut(s) 103
Ppu21I YACGTR 1 cut(s) 390
PsiI TTATAA 1 cut(s) 1241
Psp6I CCWGG 2 cut(s) 77, 477
PspCI CACGTG 1 cut(s) 390
PspGI CCWGG 2 cut(s) 77, 477
PspN4I GGNNCC 3 cut(s) 421, 1097, 1288
PsrI GAACNNNNNNTAC 2 cut(s) 479, 511
PsuI RGATCY 1 cut(s) 1286
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 5 cut(s) 374, 488, 1200, 1236, 1281
RsaNI GTAC 5 cut(s) 373, 487, 1199, 1235, 1280
RseI CAYNNNNRTG 4 cut(s) 392, 503, 848, 1219
SaqAI TTAA 3 cut(s) 639, 923, 999
SatI GCNGC 5 cut(s) 324, 687, 877, 1069, 1126
Sau3AI GATC 4 cut(s) 64, 679, 720, 1286
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 79, 479, 550
SduI GDGCHC 2 cut(s) 759, 797
SfaNI GCATC 1 cut(s) 279
SfcI CTRYAG 1 cut(s) 295
SmiMI CAYNNNNRTG 4 cut(s) 392, 503, 848, 1219
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
SphI GCATGC 1 cut(s) 25
Sse9I AATT 8 cut(s) 441, 870, 895, 962, 1052, 1266, 1273, 1329
SsiI CCGC 7 cut(s) 173, 310, 324, 450, 632, 876, 1099
SspMI CTAG 3 cut(s) 341, 971, 1065
StyD4I CCNGG 3 cut(s) 77, 477, 548
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 5 cut(s) 187, 299, 694, 727, 1092
TaiI ACGT 2 cut(s) 392, 959
TaqI TCGA 4 cut(s) 63, 98, 301, 759
TasI AATT 8 cut(s) 441, 870, 895, 962, 1052, 1266, 1273, 1329
TauI GCSGC 2 cut(s) 326, 879
TfiI GAWTC 3 cut(s) 147, 207, 275
Tru1I TTAA 3 cut(s) 639, 923, 999
Tru9I TTAA 3 cut(s) 639, 923, 999
TscAI CASTG 5 cut(s) 190, 228, 580, 730, 1152
TseFI GTSAC 3 cut(s) 394, 1318, 1342
TseI GCWGC 3 cut(s) 686, 1068, 1125
Tsp45I GTSAC 3 cut(s) 394, 1318, 1342
TspDTI ATGAA 6 cut(s) 17, 220, 288, 440, 1088, 1216
TspGWI ACGGA 1 cut(s) 927
TspRI CASTG 5 cut(s) 190, 228, 580, 730, 1152
XapI RAATTY 3 cut(s) 895, 962, 1052
XceI RCATGY 1 cut(s) 25
XcmI CCANNNNNNNNNTGG 1 cut(s) 1227
XmiI GTMKAC 1 cut(s) 245
XspI CTAG 3 cut(s) 341, 971, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.