FvH4_2g40670

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004402
Physical Location & Seq
Reverse (-)
28939345 .. 28943070
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40670.t1

Sequence Viewer

Length: 1566 bp
ATGGACGAGAGGCCGGAGACAGAGCTCATCTCGATTCCGGCGACGCCACGTGGAGTGTCCACGCCGGAGATACAGACACCCTCCGGGCAGAGGTCTCCTAGGCCGCCGTCCAAGGAGGCCAGGTCGTCGGCGTGGACTCCGACGTCGTTCATATCGCCTCGGTTCTTGAGCCCCATTGGGACTCCAATGAAGAGGGTTCTGATCAACATGAAGGGTTATTTGGAGGAGATGGGCCACCTCACCAAGCTCAACCCTCAGGATGCTTGGCTTCCCATCACTGAGTCTCGCAATGGAAACGCTCACTACGCTGCGTTTCACAACCTCAATGCTGGTGTTGGATTCCAGGCTTTGGTTCTTCCCGTTGCCTTTTCTTTTCTAGGCTGGAGTTGGGGAACACTTTCCTTAACCATTGCCTACTTCTGGCAACTGTACACATTGTGGATCCTTGTTCAGCTACATGAAGCTGTTCCCGGAAAGAGGTACAACAGATATGTGGAGCTTGCTCAGGCCGCCTTTGGGGAAAGATTGGGTGTTTGGCTTGCTCTGTTCCCAACTGTGTACCTATCAGCTGGAACTGCAACAGCTTTGATTCTCATAGGAGGAGAGACCATGAAACTATTTTTCCAGATTGTTTGCGGGCCTCTCTGTTCATCGAATCCCCTAACAACAGTGGAATGGTATCTAGTTTTCACTTCGTTGTGTATCGTCCTGTCTCAGCTTCCGAACCTCAATTCAATAGCCGGACTTTCATTAGTAGGAGCTGTCACAGCCATCACTTACTCTACAATGGTTTGGGTCCTCTCTGTAAGCCAACCAAGGCCACCCACAATGTCTTATGAACCACTTCCAATGCCATCTTTCTCGGCTTCCCTCTTTGCAGCCTTGAACGCACTTGGTATAATAGCCTTTGCATTCAGAGGTCACAATTTAGTTCTAGAGATTCAGGCAACAATGCCTTCAACCTTTAAACACCCAGCTCATGTACCCATGTGGAAAGGAGCAAAGGTTGCCTATGTCTTGATTGCCTTGTGTGTGTTTCCTGTTGCAATCGGAGGCTTCTGGGCCTATGGAAACCTCATGCCGTCTGGAGGGATATTGAATGCACTGTACGGATTCCATAGTCATGACATCCCCAGAGGATTACTTGCAATGTGTTTCCTACTAGTTGTGTTCAATTGTTTGAGCAGCTTTCAGATATACTCCATGCCCGTATTTGATAGTTTTGAAGCTGGTTACACTAGTCGTACCAACCGACCCTGTTCAATCTGGGTCCGTTCTGGTTTTCGAGTTTTCTACGGCTTCATCTCATTCTTCATAGGAGTGGCACTCCCCTTCCTGTCCAGTCTTGCTGGGCTGTTGGGAGGACTAACCCTCCCAGTCACATTTGCCTACCCTTGTTTCATGTGGGTTCTCATAAAAAAGCCTACAAAGTACAGCTTCAATTGGTATTTCAATTGGATCTTAGGCTGGTTGGGGATTGCCTTCAGTTTGGCCTTTTCAATTGGAGGCATTTGGAGTATGGTCAACAGTGGACTTAAATTGAAGTTCTTCAAGCCGCCTAGCTGA

Protein Analysis

522

Amino Acids

57.54

Weight (kDa)

9.36

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 97 - 508 3e-60 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 142
AatII GACGTC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 349
AciI CCGC 4 cut(s) 104, 510, 636, 1556
AclWI GGATC 3 cut(s) 436, 449, 1466
AcuI CTGAAG 1 cut(s) 1468
AcvI CACGTG 1 cut(s) 50
AcyI GRCGYC 2 cut(s) 44, 143
AdeI CACNNNGTG 1 cut(s) 438
AfaI GTAC 7 cut(s) 431, 482, 560, 984, 1109, 1246, 1433
AfiI CCNNNNNNNGG 6 cut(s) 90, 349, 420, 477, 516, 1088
AhlI ACTAGT 2 cut(s) 1162, 1238
AjnI CCWGG 2 cut(s) 119, 342
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 5 cut(s) 11, 99, 288, 599, 717
AlwI GGATC 3 cut(s) 436, 449, 1466
AoxI GGCC 9 cut(s) 11, 101, 117, 232, 507, 638, 818, 1062, 1491
ApeKI GCWGC 3 cut(s) 308, 878, 1185
Asp700I GAANNNNTTC 4 cut(s) 397, 465, 843, 1547
AspA2I CCTAGG 1 cut(s) 98
AspS9I GGNCC 5 cut(s) 232, 638, 796, 1062, 1270
AsuC2I CCSGG 2 cut(s) 85, 471
AsuHPI GGTGA 1 cut(s) 232
AvaII GGWCC 2 cut(s) 796, 1270
AvrII CCTAGG 1 cut(s) 98
AxyI CCTNAGG 1 cut(s) 255
BamHI GGATCC 1 cut(s) 441
BanII GRGCYC 2 cut(s) 27, 173
BbrPI CACGTG 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 3 cut(s) 295, 890, 1197
BccI CCATC 4 cut(s) 223, 281, 779, 862
BceAI ACGGC 3 cut(s) 91, 1066, 1312
BciT130I CCWGG 2 cut(s) 121, 344
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 2 cut(s) 85, 471
BcoDI GTCTC 5 cut(s) 11, 99, 288, 599, 717
BcuI ACTAGT 2 cut(s) 1162, 1238
BfaI CTAG 7 cut(s) 99, 377, 683, 935, 1163, 1239, 1560
BisI GCNGC 6 cut(s) 104, 309, 510, 879, 1186, 1556
BlnI CCTAGG 1 cut(s) 98
BlsI GCNGC 6 cut(s) 105, 310, 511, 880, 1187, 1557
Bme1390I CCNGG 4 cut(s) 85, 121, 344, 471
Bme18I GGWCC 2 cut(s) 796, 1270
BmgT120I GGNCC 5 cut(s) 232, 638, 796, 1062, 1270
BmiI GGNNCC 3 cut(s) 443, 797, 1271
BmrFI CCNGG 4 cut(s) 85, 121, 344, 471
BmrI ACTGGG 1 cut(s) 1370
BmsI GCATC 1 cut(s) 250
BmuI ACTGGG 1 cut(s) 1370
BplI GAGNNNNNCTC 4 cut(s) 14, 46, 1311, 1343
BpmI CTGGAG 2 cut(s) 403, 1107
Bpu10I CCTNAGC 1 cut(s) 504
BpuEI CTTGAG 1 cut(s) 187
BpuMI CCSGG 2 cut(s) 85, 471
BsaAI YACGTR 1 cut(s) 50
BsaHI GRCGYC 2 cut(s) 44, 143
BsaI GGTCTC 2 cut(s) 99, 599
BsaJI CCNNGG 4 cut(s) 98, 111, 158, 815
BsaXI ACNNNNNCTCC 6 cut(s) 107, 137, 990, 1020, 1356, 1386
Bsc4I CCNNNNNNNGG 6 cut(s) 90, 349, 420, 477, 516, 1088
Bse1I ACTGG 2 cut(s) 1341, 1376
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 3 cut(s) 295, 408, 1155
BseBI CCWGG 2 cut(s) 121, 344
BseDI CCNNGG 4 cut(s) 98, 111, 158, 815
BseGI GGATG 2 cut(s) 265, 1128
BseLI CCNNNNNNNGG 6 cut(s) 90, 349, 420, 477, 516, 1088
BseMI GCAATG 3 cut(s) 295, 408, 1155
BseMII CTCAG 4 cut(s) 269, 270, 518, 728
BseNI ACTGG 2 cut(s) 1341, 1376
BseRI GAGGAG 2 cut(s) 239, 615
BseXI GCAGC 3 cut(s) 295, 890, 1197
BseYI CCCAGC 2 cut(s) 973, 1349
BshFI GGCC 9 cut(s) 13, 103, 119, 234, 509, 640, 820, 1064, 1493
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 6 cut(s) 14, 38, 65, 84, 471, 741
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 6 cut(s) 90, 349, 420, 477, 516, 1088
BsmAI GTCTC 5 cut(s) 11, 99, 288, 599, 717
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 2 cut(s) 911, 1105
BsnI GGCC 9 cut(s) 13, 103, 119, 234, 509, 640, 820, 1064, 1493
Bso31I GGTCTC 2 cut(s) 99, 599
Bsp1286I GDGCHC 2 cut(s) 27, 173
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 3 cut(s) 201, 441, 1458
BspACI CCGC 4 cut(s) 104, 510, 636, 1556
BspANI GGCC 9 cut(s) 13, 103, 119, 234, 509, 640, 820, 1064, 1493
BspCNI CTCAG 4 cut(s) 268, 271, 517, 727
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 3 cut(s) 443, 797, 1271
BspPI GGATC 3 cut(s) 436, 449, 1466
BspTNI GGTCTC 2 cut(s) 99, 599
BsrDI GCAATG 3 cut(s) 295, 408, 1155
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 2 cut(s) 1341, 1376
BssECI CCNNGG 4 cut(s) 98, 111, 158, 815
BssMI GATC 3 cut(s) 201, 441, 1458
BssNI GRCGYC 2 cut(s) 44, 143
BssT1I CCWWGG 3 cut(s) 98, 111, 815
Bst2UI CCWGG 2 cut(s) 121, 344
Bst4CI ACNGT 5 cut(s) 429, 556, 670, 1107, 1529
Bst6I CTCTTC 1 cut(s) 185
BstACI GRCGYC 2 cut(s) 44, 143
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstAUI TGTACA 1 cut(s) 429
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 3 cut(s) 501, 540, 638
BstDEI CTNAG 5 cut(s) 255, 279, 504, 714, 1462
BstF5I GGATG 2 cut(s) 265, 1128
BstKTI GATC 3 cut(s) 204, 444, 1461
BstMAI GTCTC 5 cut(s) 11, 99, 288, 599, 717
BstMBI GATC 3 cut(s) 201, 441, 1458
BstMWI GCNNNNNNNGC 6 cut(s) 305, 509, 575, 767, 887, 1007
BstNI CCWGG 2 cut(s) 121, 344
BstSCI CCNGG 4 cut(s) 83, 119, 342, 469
BstV1I GCAGC 3 cut(s) 295, 890, 1197
BstX2I RGATCY 2 cut(s) 441, 1458
BstYI RGATCY 2 cut(s) 441, 1458
Bsu36I CCTNAGG 1 cut(s) 255
BsuRI GGCC 9 cut(s) 13, 103, 119, 234, 509, 640, 820, 1064, 1493
BtsCI GGATG 2 cut(s) 265, 1128
BtsIMutI CAGTG 4 cut(s) 276, 675, 1103, 1534
Cac8I GCNNGC 3 cut(s) 501, 540, 638
CciI TCATGA 1 cut(s) 1123
Cfr13I GGNCC 5 cut(s) 232, 638, 796, 1062, 1270
CseI GACGC 1 cut(s) 52
Csp6I GTAC 7 cut(s) 430, 481, 559, 983, 1108, 1245, 1432
CviAII CATG 9 cut(s) 208, 458, 610, 980, 988, 1078, 1124, 1204, 1402
CviQI GTAC 7 cut(s) 430, 481, 559, 983, 1108, 1245, 1432
DdeI CTNAG 5 cut(s) 255, 279, 504, 714, 1462
DpnI GATC 3 cut(s) 203, 443, 1460
DpnII GATC 3 cut(s) 201, 441, 1458
DraI TTTAAA 1 cut(s) 967
DraIII CACNNNGTG 1 cut(s) 438
DrdI GACNNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 3 cut(s) 98, 111, 815
Eco24I GRGCYC 2 cut(s) 27, 173
Eco31I GGTCTC 2 cut(s) 99, 599
Eco47I GGWCC 2 cut(s) 796, 1270
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 1468
Eco72I CACGTG 1 cut(s) 50
Eco81I CCTNAGG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 796
EcoRII CCWGG 2 cut(s) 119, 342
EcoT14I CCWWGG 3 cut(s) 98, 111, 815
EcoT38I GRGCYC 2 cut(s) 27, 173
ErhI CCWWGG 3 cut(s) 98, 111, 815
FaeI CATG 9 cut(s) 211, 461, 613, 983, 991, 1081, 1127, 1207, 1405
FalI AAGNNNNNCTT 2 cut(s) 1421, 1453
FaqI GGGAC 1 cut(s) 193
FatI CATG 9 cut(s) 207, 457, 609, 979, 987, 1077, 1123, 1203, 1401
FauI CCCGC 1 cut(s) 629
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 6 cut(s) 104, 309, 510, 879, 1186, 1556
FokI GGATG 2 cut(s) 272, 1115
FriOI GRGCYC 2 cut(s) 27, 173
Fsp4HI GCNGC 6 cut(s) 104, 309, 510, 879, 1186, 1556
FspBI CTAG 7 cut(s) 99, 377, 683, 935, 1163, 1239, 1560
GluI GCNGC 6 cut(s) 104, 309, 510, 879, 1186, 1556
GsaI CCCAGC 2 cut(s) 977, 1353
GsuI CTGGAG 2 cut(s) 403, 1107
HaeIII GGCC 9 cut(s) 13, 103, 119, 234, 509, 640, 820, 1064, 1493
HapII CCGG 6 cut(s) 14, 38, 65, 84, 471, 741
HgaI GACGC 1 cut(s) 52
Hin1I GRCGYC 2 cut(s) 44, 143
Hin1II CATG 9 cut(s) 211, 461, 613, 983, 991, 1081, 1127, 1207, 1405
HincII GTYRAC 1 cut(s) 1525
HindII GTYRAC 1 cut(s) 1525
HinfI GANTC 9 cut(s) 34, 136, 181, 281, 339, 589, 655, 940, 1113
HpaII CCGG 6 cut(s) 14, 38, 65, 84, 471, 741
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 6 cut(s) 60, 135, 432, 559, 1525, 1532
Hpy188I TCNGA 6 cut(s) 141, 201, 723, 917, 1052, 1194
Hpy188III TCNNGA 8 cut(s) 31, 166, 257, 625, 935, 1018, 1086, 1124
Hpy8I GTNNAC 6 cut(s) 60, 135, 432, 559, 1525, 1532
Hpy99I CGWCG 4 cut(s) 46, 130, 145, 148
HpyAV CCTTC 4 cut(s) 205, 966, 1342, 1492
HpyCH4III ACNGT 5 cut(s) 429, 556, 670, 1107, 1529
HpyCH4IV ACGT 2 cut(s) 49, 143
HpyCH4V TGCA 6 cut(s) 578, 878, 911, 1046, 1103, 1148
HpyF10VI GCNNNNNNNGC 6 cut(s) 305, 509, 575, 767, 887, 1007
HpyF3I CTNAG 5 cut(s) 255, 279, 504, 714, 1462
HpySE526I ACGT 2 cut(s) 49, 143
Hsp92I GRCGYC 2 cut(s) 44, 143
Hsp92II CATG 9 cut(s) 211, 461, 613, 983, 991, 1081, 1127, 1207, 1405
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 3 cut(s) 201, 441, 1458
LmnI GCTCC 3 cut(s) 496, 758, 998
Lsp1109I GCAGC 3 cut(s) 295, 890, 1197
LweI GCATC 1 cut(s) 250
MaeI CTAG 7 cut(s) 99, 377, 683, 935, 1163, 1239, 1560
MaeII ACGT 2 cut(s) 49, 143
MaeIII GTNAC 4 cut(s) 763, 920, 1232, 1378
MalI GATC 3 cut(s) 203, 443, 1460
MboI GATC 3 cut(s) 201, 441, 1458
MboII GAAGA 4 cut(s) 202, 347, 1303, 1540
MfeI CAATTG 4 cut(s) 1174, 1441, 1453, 1500
MflI RGATCY 2 cut(s) 441, 1458
MhlI GDGCHC 2 cut(s) 27, 173
MluCI AATT 7 cut(s) 730, 925, 1174, 1441, 1453, 1500, 1538
MlyI GAGTC 3 cut(s) 130, 175, 290
MmeI TCCRAC 2 cut(s) 164, 316
MroXI GAANNNNTTC 4 cut(s) 397, 465, 843, 1547
MseI TTAA 3 cut(s) 404, 966, 1536
MslI CAYNNNNRTG 2 cut(s) 1122, 1319
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 6 cut(s) 14, 38, 65, 84, 471, 741
MspR9I CCNGG 4 cut(s) 85, 121, 344, 471
MunI CAATTG 4 cut(s) 1174, 1441, 1453, 1500
Mva1269I GAATGC 2 cut(s) 911, 1105
MvaI CCWGG 2 cut(s) 121, 344
MwoI GCNNNNNNNGC 6 cut(s) 305, 509, 575, 767, 887, 1007
NciI CCSGG 2 cut(s) 85, 471
NdeII GATC 3 cut(s) 201, 441, 1458
NlaIII CATG 9 cut(s) 211, 461, 613, 983, 991, 1081, 1127, 1207, 1405
NlaIV GGNNCC 3 cut(s) 443, 797, 1271
NmeAIII GCCGAG 1 cut(s) 842
NmuCI GTSAC 3 cut(s) 763, 920, 1378
PagI TCATGA 1 cut(s) 1123
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 1249
PctI GAATGC 2 cut(s) 911, 1105
PdmI GAANNNNTTC 4 cut(s) 397, 465, 843, 1547
PfeI GAWTC 6 cut(s) 34, 339, 589, 655, 940, 1113
PflMI CCANNNNNTGG 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 6 cut(s) 105, 310, 511, 880, 1187, 1557
PleI GAGTC 3 cut(s) 130, 175, 289
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 3 cut(s) 130, 175, 289
Ppu21I YACGTR 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 796
Psp124BI GAGCTC 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 796
Psp6I CCWGG 2 cut(s) 119, 342
PspCI CACGTG 1 cut(s) 50
PspFI CCCAGC 2 cut(s) 973, 1349
PspGI CCWGG 2 cut(s) 119, 342
PspN4I GGNNCC 3 cut(s) 443, 797, 1271
PspPI GGNCC 5 cut(s) 232, 638, 796, 1062, 1270
PspPPI RGGWCCY 1 cut(s) 796
PsuI RGATCY 2 cut(s) 441, 1458
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 7 cut(s) 431, 482, 560, 984, 1109, 1246, 1433
RsaNI GTAC 7 cut(s) 430, 481, 559, 983, 1108, 1245, 1432
RseI CAYNNNNRTG 2 cut(s) 1122, 1319
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 3 cut(s) 404, 966, 1536
SatI GCNGC 6 cut(s) 104, 309, 510, 879, 1186, 1556
Sau3AI GATC 3 cut(s) 201, 441, 1458
Sau96I GGNCC 5 cut(s) 232, 638, 796, 1062, 1270
SchI GAGTC 3 cut(s) 130, 175, 290
ScrFI CCNGG 4 cut(s) 85, 121, 344, 471
SduI GDGCHC 2 cut(s) 27, 173
SfaNI GCATC 1 cut(s) 250
SinI GGWCC 2 cut(s) 796, 1270
SmiMI CAYNNNNRTG 2 cut(s) 1122, 1319
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SpeI ACTAGT 2 cut(s) 1162, 1238
Sse9I AATT 7 cut(s) 730, 925, 1174, 1441, 1453, 1500, 1538
SsiI CCGC 4 cut(s) 104, 510, 636, 1556
SspMI CTAG 7 cut(s) 99, 377, 683, 935, 1163, 1239, 1560
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 4 cut(s) 83, 119, 342, 469
StyI CCWWGG 3 cut(s) 98, 111, 815
TaaI ACNGT 5 cut(s) 429, 556, 670, 1107, 1529
TaiI ACGT 2 cut(s) 52, 146
TaqI TCGA 3 cut(s) 32, 653, 1285
TasI AATT 7 cut(s) 730, 925, 1174, 1441, 1453, 1500, 1538
TatI WGTACW 2 cut(s) 429, 1431
TauI GCSGC 3 cut(s) 106, 512, 1558
TfiI GAWTC 6 cut(s) 34, 339, 589, 655, 940, 1113
Tru1I TTAA 3 cut(s) 404, 966, 1536
Tru9I TTAA 3 cut(s) 404, 966, 1536
TscAI CASTG 4 cut(s) 283, 675, 1110, 1534
TseFI GTSAC 3 cut(s) 763, 920, 1378
TseI GCWGC 3 cut(s) 308, 878, 1185
Tsp45I GTSAC 3 cut(s) 763, 920, 1378
TspGWI ACGGA 2 cut(s) 1125, 1262
TspRI CASTG 4 cut(s) 283, 675, 1110, 1534
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 796, 1270
XbaI TCTAGA 1 cut(s) 934
XmaJI CCTAGG 1 cut(s) 98
XmnI GAANNNNTTC 4 cut(s) 397, 465, 843, 1547
XspI CTAG 7 cut(s) 99, 377, 683, 935, 1163, 1239, 1560
ZraI GACGTC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.