MD08G1004200.v1.1

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004402
Physical Location & Seq
Forward (+)
404340 .. 405913
1574 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1004200.v1.1.491

Sequence Viewer

Length: 933 bp
ATGCAGGATCAGATGGATGAGGTGGAGGAAGTGAAGTCGAGCCCAATGAGTAATCCAAACATGACCCCTAAAAGTCCGTTCGTTTCTCGTTTTATGGCGACTCCTTTGGCGAGTCCGATGAAGAAGGCGATGACAAGCATGCGAGGCTACCTAGAAGAAGTTGGACACTTGACAAAGCTTGATCCTCAGGACGCATGGCTGCCCATCACCGAGTCCAGAAACGGAAATGCCCTCTACTCCGCCTTTCACACGCTCAGTTCCGGAATCGGCATTCCAGCCCTCCTTCTTCCTTTATCTTTCGCTACTCTTGGCTGGGCATGGGGAATTATATGTCTCTCGGTGGCTTTCATGTGGCAGCTCTATACTCTCTGGTTACTGATACAACTGCACGAATCCGAATCGGGAGTGCGCTACAGCAGATACCTTCGACTTTCCATGGCAGCTTTTGGTGAGAAGCTAGGGAAAATTCTGGCATTGTTTCCAATCATGTACCTCTCAGGTGGTACGTGTGTAACCCTGATCATGATCGGAGGTGGGACCATGAAAATATTTTTCCAAATCGTGTGCGGTGAGACATGCCGCCATGTAAGTCCACCGTCAACGATAGAGTGGTACTTGGTGTTCACCGGCACTGCCATTCTTCTCGCTCAGCTTCCTAACCTCAACTCAATAGCTGGGATTTCCCTCATTGGAGCTATCACCGCCGTAAGCTACTGCACACTCATTTGGGTGGTTTCTGTCACCAAAGATAGACCCAATGGTGTTTCTTATAAGCCGCTGGAAACAAAATCTGATGCAGCCACCGTTTTTGGCGTCCTAAATGCGCTTGGGATCACTATCTTTGCTTTTAGAGGCCACAACCTTGTGCTTGAAATACAGGTAATGTATATACATATGAAGTTTCTCTACGTGCACACATGGTCGAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

311

Amino Acids

34.3

Weight (kDa)

8.53

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 74 - 293 1.6e-28 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 771
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 5 cut(s) 240, 567, 580, 702, 776
AclWI GGATC 3 cut(s) 15, 176, 839
AcsI RAATTY 1 cut(s) 465
AcyI GRCGYC 1 cut(s) 813
AfaI GTAC 3 cut(s) 491, 505, 614
AflIII ACRYGT 1 cut(s) 506
AgsI TTSAA 1 cut(s) 872
AluBI AGCT 8 cut(s) 178, 358, 443, 457, 652, 674, 695, 711
AluI AGCT 8 cut(s) 178, 358, 443, 457, 652, 674, 695, 711
Alw21I GWGCWC 1 cut(s) 915
Alw26I GTCTC 2 cut(s) 338, 566
Alw44I GTGCAC 1 cut(s) 911
AlwI GGATC 3 cut(s) 15, 176, 839
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 1 cut(s) 853
ApaLI GTGCAC 1 cut(s) 911
ApeKI GCWGC 4 cut(s) 199, 355, 440, 797
ApoI RAATTY 1 cut(s) 465
AspLEI GCGC 2 cut(s) 411, 826
AspS9I GGNCC 1 cut(s) 537
AsuHPI GGTGA 6 cut(s) 199, 461, 581, 616, 691, 733
AvaII GGWCC 1 cut(s) 537
AxyI CCTNAGG 1 cut(s) 186
BaeGI GKGCMC 1 cut(s) 915
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 2 cut(s) 890, 922
Bbv12I GWGCWC 1 cut(s) 915
BbvI GCAGC 4 cut(s) 186, 367, 452, 809
BccI CCATC 2 cut(s) 7, 212
BceAI ACGGC 1 cut(s) 689
BclI TGATCA 1 cut(s) 519
BcoDI GTCTC 2 cut(s) 338, 566
BfaI CTAG 2 cut(s) 152, 458
BfmI CTRYAG 1 cut(s) 412
BisI GCNGC 6 cut(s) 200, 356, 441, 580, 776, 798
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 6 cut(s) 201, 357, 442, 581, 777, 799
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 1 cut(s) 538
BmsI GCATC 1 cut(s) 784
Bpu1102I GCTNAGC 1 cut(s) 648
BsaAI YACGTR 2 cut(s) 507, 910
BsaBI GATNNNNATC 2 cut(s) 524, 836
BsaHI GRCGYC 1 cut(s) 813
BsaJI CCNNGG 1 cut(s) 435
BsaWI WCCGGW 1 cut(s) 260
Bse118I RCCGGY 1 cut(s) 626
Bse21I CCTNAGG 1 cut(s) 186
Bse8I GATNNNNATC 2 cut(s) 524, 836
BseAI TCCGGA 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 2 cut(s) 524, 836
BseMII CTCAG 4 cut(s) 200, 268, 510, 662
BseSI GKGCMC 1 cut(s) 915
BseXI GCAGC 4 cut(s) 186, 367, 452, 809
BseYI CCCAGC 2 cut(s) 312, 674
BsgI GTGCAG 2 cut(s) 371, 700
BshFI GGCC 1 cut(s) 855
BsiHKAI GWGCWC 1 cut(s) 915
BsiSI CCGG 2 cut(s) 261, 627
BslFI GGGAC 1 cut(s) 550
BsmAI GTCTC 2 cut(s) 338, 566
BsmFI GGGAC 1 cut(s) 550
BsmI GAATGC 1 cut(s) 270
BsnI GGCC 1 cut(s) 855
Bsp1286I GDGCHC 2 cut(s) 44, 915
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 5 cut(s) 7, 181, 519, 525, 831
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 435
BspACI CCGC 5 cut(s) 240, 567, 580, 702, 776
BspANI GGCC 1 cut(s) 855
BspCNI CTCAG 4 cut(s) 199, 267, 509, 661
BspEI TCCGGA 1 cut(s) 260
BspHI TCATGA 1 cut(s) 522
BspLI GGNNCC 1 cut(s) 538
BspPI GGATC 3 cut(s) 15, 176, 839
BsrFI RCCGGY 1 cut(s) 626
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 5 cut(s) 7, 181, 519, 525, 831
BssNI GRCGYC 1 cut(s) 813
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 3 cut(s) 597, 805, 930
BstACI GRCGYC 1 cut(s) 813
BstBAI YACGTR 2 cut(s) 507, 910
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 4 cut(s) 186, 254, 496, 648
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 1 cut(s) 22
BstHHI GCGC 2 cut(s) 411, 826
BstKTI GATC 5 cut(s) 10, 184, 522, 528, 834
BstMAI GTCTC 2 cut(s) 338, 566
BstMBI GATC 5 cut(s) 7, 181, 519, 525, 831
BstMWI GCNNNNNNNGC 2 cut(s) 144, 701
BstNSI RCATGY 2 cut(s) 142, 579
BstSFI CTRYAG 1 cut(s) 412
BstSLI GKGCMC 1 cut(s) 915
BstV1I GCAGC 4 cut(s) 186, 367, 452, 809
Bsu36I CCTNAGG 1 cut(s) 186
BsuRI GGCC 1 cut(s) 855
BtgI CCRYGG 1 cut(s) 435
BtgZI GCGATG 1 cut(s) 143
BtsCI GGATG 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 1 cut(s) 630
Cac8I GCNNGC 1 cut(s) 140
CciI TCATGA 1 cut(s) 522
CfoI GCGC 2 cut(s) 411, 826
Cfr10I RCCGGY 1 cut(s) 626
Cfr13I GGNCC 1 cut(s) 537
CseI GACGC 2 cut(s) 200, 802
Csp6I GTAC 3 cut(s) 490, 504, 613
CspCI CAANNNNNGTGG 2 cut(s) 790, 825
CviQI GTAC 3 cut(s) 490, 504, 613
DdeI CTNAG 4 cut(s) 186, 254, 496, 648
DpnI GATC 5 cut(s) 9, 183, 521, 527, 833
DpnII GATC 5 cut(s) 7, 181, 519, 525, 831
EciI GGCGGA 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 537
Eco81I CCTNAGG 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 435
FaqI GGGAC 1 cut(s) 550
FauNDI CATATG 1 cut(s) 894
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 6 cut(s) 200, 356, 441, 580, 776, 798
FokI GGATG 1 cut(s) 29
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 6 cut(s) 200, 356, 441, 580, 776, 798
FspBI CTAG 2 cut(s) 152, 458
GlaI GCGC 2 cut(s) 410, 825
GluI GCNGC 6 cut(s) 200, 356, 441, 580, 776, 798
GsaI CCCAGC 2 cut(s) 316, 678
HaeIII GGCC 1 cut(s) 855
HapII CCGG 2 cut(s) 261, 627
HgaI GACGC 2 cut(s) 200, 802
HhaI GCGC 2 cut(s) 411, 826
Hin1I GRCGYC 1 cut(s) 813
Hin6I GCGC 2 cut(s) 409, 824
HinP1I GCGC 2 cut(s) 409, 824
HincII GTYRAC 1 cut(s) 600
HindII GTYRAC 1 cut(s) 600
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 6 cut(s) 100, 112, 212, 264, 392, 398
HpaII CCGG 2 cut(s) 261, 627
HphI GGTGA 6 cut(s) 199, 461, 581, 616, 691, 733
Hpy166II GTNNAC 4 cut(s) 593, 600, 624, 913
Hpy188I TCNGA 5 cut(s) 12, 117, 397, 530, 793
Hpy188III TCNNGA 5 cut(s) 188, 216, 261, 402, 523
Hpy8I GTNNAC 4 cut(s) 593, 600, 624, 913
HpyAV CCTTC 3 cut(s) 118, 293, 434
HpyCH4III ACNGT 3 cut(s) 597, 805, 930
HpyCH4IV ACGT 2 cut(s) 506, 909
HpyCH4V TGCA 5 cut(s) 4, 388, 717, 797, 913
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 701
HpyF3I CTNAG 4 cut(s) 186, 254, 496, 648
HpySE526I ACGT 2 cut(s) 506, 909
Hsp92I GRCGYC 1 cut(s) 813
HspAI GCGC 2 cut(s) 409, 824
Kpn2I TCCGGA 1 cut(s) 260
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 5 cut(s) 7, 181, 519, 525, 831
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 4 cut(s) 186, 367, 452, 809
LweI GCATC 1 cut(s) 784
MaeI CTAG 2 cut(s) 152, 458
MaeII ACGT 2 cut(s) 506, 909
MaeIII GTNAC 3 cut(s) 372, 511, 739
MalI GATC 5 cut(s) 9, 183, 521, 527, 833
MboI GATC 5 cut(s) 7, 181, 519, 525, 831
MboII GAAGA 4 cut(s) 133, 167, 278, 632
MhlI GDGCHC 2 cut(s) 44, 915
MluCI AATT 2 cut(s) 324, 465
MlyI GAGTC 3 cut(s) 94, 121, 221
MmeI TCCRAC 1 cut(s) 142
MroI TCCGGA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 728
MspA1I CMGCKG 1 cut(s) 778
MspI CCGG 2 cut(s) 261, 627
Mva1269I GAATGC 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 144, 701
NcoI CCATGG 1 cut(s) 435
NdeI CATATG 1 cut(s) 894
NdeII GATC 5 cut(s) 7, 181, 519, 525, 831
NlaIV GGNNCC 1 cut(s) 538
NmuCI GTSAC 1 cut(s) 739
NspI RCATGY 2 cut(s) 142, 579
PaeI GCATGC 1 cut(s) 142
PagI TCATGA 1 cut(s) 522
PctI GAATGC 1 cut(s) 270
PfeI GAWTC 3 cut(s) 264, 392, 398
PkrI GCNGC 6 cut(s) 201, 357, 442, 581, 777, 799
PleI GAGTC 3 cut(s) 94, 120, 220
PpsI GAGTC 3 cut(s) 94, 120, 220
Ppu21I YACGTR 2 cut(s) 507, 910
PsiI TTATAA 1 cut(s) 771
PspFI CCCAGC 2 cut(s) 312, 674
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 1 cut(s) 537
PsrI GAACNNNNNNTAC 2 cut(s) 605, 637
RsaI GTAC 3 cut(s) 491, 505, 614
RsaNI GTAC 3 cut(s) 490, 504, 613
RseI CAYNNNNRTG 1 cut(s) 728
SatI GCNGC 6 cut(s) 200, 356, 441, 580, 776, 798
Sau3AI GATC 5 cut(s) 7, 181, 519, 525, 831
Sau96I GGNCC 1 cut(s) 537
SchI GAGTC 3 cut(s) 94, 121, 221
SduI GDGCHC 2 cut(s) 44, 915
SfaNI GCATC 1 cut(s) 784
SfcI CTRYAG 1 cut(s) 412
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 728
SphI GCATGC 1 cut(s) 142
Sse9I AATT 2 cut(s) 324, 465
SsiI CCGC 5 cut(s) 240, 567, 580, 702, 776
SspI AATATT 1 cut(s) 549
SspMI CTAG 2 cut(s) 152, 458
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 3 cut(s) 597, 805, 930
TaiI ACGT 2 cut(s) 509, 912
TaqI TCGA 3 cut(s) 38, 427, 923
TasI AATT 2 cut(s) 324, 465
TauI GCSGC 2 cut(s) 582, 778
TfiI GAWTC 3 cut(s) 264, 392, 398
TscAI CASTG 1 cut(s) 637
TseFI GTSAC 1 cut(s) 739
TseI GCWGC 4 cut(s) 199, 355, 440, 797
Tsp45I GTSAC 1 cut(s) 739
TspDTI ATGAA 4 cut(s) 134, 337, 557, 911
TspGWI ACGGA 2 cut(s) 66, 237
TspRI CASTG 1 cut(s) 637
VneI GTGCAC 1 cut(s) 911
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 1 cut(s) 465
XceI RCATGY 2 cut(s) 142, 579
XspI CTAG 2 cut(s) 152, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.