Rroxscaffold_7G00168220

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004402
Physical Location & Seq
Reverse (-)
9122719 .. 9125463
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00168220.1

Sequence Viewer

Length: 1173 bp
ATGGACGAGAGGCCCGGAGACAGAGCTCATCTCGATTCCGGCTACGCCGCGTGGAGTGTCCACGCCGGAGATACAGACACCCCTCAGGACAGAGGTCGCCTAGGCCGCCATCCAAGGAGGCCAAGTCGTCGGCGTGGACTCCGACGTCGTTCATATCCCCTAGGAGTTGGGGAACGCTTTCCTTTAACCATAGCCTACTTTTGGCAACTATACACATTGTGGATTTTAGTTCAGCTACATGAAGCTGTTCCCGGAAAGAGGTACAACAGATATGTGGAGCTTGCTCAAGCAGCCTTTGGGGAAAGATTGGGTGTTTGGCTTGCTCTGTTCCCAACTGTATACCTATCAGCAGGAACTGCAACAGCTTTGATTCTCATAGGAGGAGAGACCATGAAACTATTTTTCCAGATTCTTCCAAACCTCAATTCAATAGCCGGACTTTCATTAGTAGGAGCTGTCACAGCCATCACTTACTCCACAATGGTTTGGGTCCTCTCTGTAAGCCAACCAAGGCCACCCACAATTTCTTATGAACCGCTTCCATTGCCATCTTTCTCTGCTTCCCTCTTTGCAGCCTTGAACGCACTTGGTATTATAGCCTTTGCATTCAGAGGCCACAATTTAGTTCTAGAGATTCAGGCAACAATGCCATCAACCTTTAAGCACCCAGCTCATGTACCCATGTGGAAAGGTGCAAAGGTTGCCCTATTTCTTCATTGCCATGTGTGTGTTCCCTGTTGCGATTGGGAGGCTTCTGGGCTTATGGAAACCTTATACAAAAATATGCCTTCTGGAGGGATACTGAATGCATTATACGCGTTCCACAGTCATGACATCCCCAGAGGGCTACTGGCAATGAGTTTCCTGCTAGTTGTGTTCAATTGTTTGAGCAGTTTTCAGATATACTCTATGCCCGTATTCGACAGTGTGGCACTCCCCTTCCTGTCTAGTCTTGCTGGACTATTGGGAGGACTGACCCTTCCAGTCACATTTGCTTACCCTTGCTTCATGTGGGTTCTCATAAAAAGCCCACAAAGTACAGCTTTAATTGGTATTTCAATTGGGATCTTGGGCTGGTTGGGGATTGCCTTTAGTTTGGCTTTTTCAATTGGAGGCATTTGGAGTATGGTCAACAGTGGACTTAAATTGAAGTTCTTCAAGCCGCCTAGCTGA

Protein Analysis

390

Amino Acids

42.85

Weight (kDa)

9.49

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 137 - 240 3.6e-11 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 144
AatII GACGTC 1 cut(s) 148
AccI GTMKAC 1 cut(s) 339
AccII CGCG 2 cut(s) 50, 818
AciI CCGC 4 cut(s) 48, 106, 536, 1163
AclWI GGATC 1 cut(s) 1073
AcyI GRCGYC 1 cut(s) 145
AdeI CACNNNGTG 1 cut(s) 219
AfaI GTAC 3 cut(s) 263, 678, 1039
AfiI CCNNNNNNNGG 3 cut(s) 201, 258, 794
AflIII ACRYGT 1 cut(s) 816
AgsI TTSAA 7 cut(s) 429, 580, 880, 1059, 1107, 1150, 1159
AjuI GAANNNNNNNTTGG 2 cut(s) 409, 441
AluBI AGCT 9 cut(s) 26, 235, 245, 280, 365, 455, 671, 1043, 1170
AluI AGCT 9 cut(s) 26, 235, 245, 280, 365, 455, 671, 1043, 1170
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 2 cut(s) 12, 380
AlwI GGATC 1 cut(s) 1073
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 5 cut(s) 11, 103, 119, 512, 613
ApeKI GCWGC 2 cut(s) 290, 572
Asp700I GAANNNNTTC 4 cut(s) 177, 246, 537, 1154
AspA2I CCTAGG 2 cut(s) 100, 160
AspS9I GGNCC 2 cut(s) 12, 490
AsuC2I CCSGG 2 cut(s) 15, 252
AvaII GGWCC 1 cut(s) 490
AvrII CCTAGG 2 cut(s) 100, 160
AxyI CCTNAGG 1 cut(s) 84
BanII GRGCYC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 2 cut(s) 302, 584
BccI CCATC 4 cut(s) 117, 473, 556, 658
BciVI GTATCC 1 cut(s) 792
BcnI CCSGG 2 cut(s) 15, 252
BcoDI GTCTC 2 cut(s) 12, 380
BfaI CTAG 6 cut(s) 101, 161, 629, 869, 948, 1167
BfuI GTATCC 1 cut(s) 792
BisI GCNGC 5 cut(s) 48, 106, 291, 573, 1163
BlnI CCTAGG 2 cut(s) 100, 160
BlsI GCNGC 5 cut(s) 49, 107, 292, 574, 1164
Bme1390I CCNGG 2 cut(s) 15, 252
Bme18I GGWCC 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 12, 490
BmiI GGNNCC 1 cut(s) 491
BmrFI CCNGG 2 cut(s) 15, 252
BoxI GACNNNNGTC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 15, 47
BpmI CTGGAG 1 cut(s) 813
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 2 cut(s) 15, 252
BsaHI GRCGYC 1 cut(s) 145
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 4 cut(s) 100, 113, 160, 509
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 258, 794
Bse1I ACTGG 2 cut(s) 855, 983
Bse21I CCTNAGG 1 cut(s) 84
Bse3DI GCAATG 3 cut(s) 542, 715, 861
BseDI CCNNGG 4 cut(s) 100, 113, 160, 509
BseGI GGATG 2 cut(s) 109, 834
BseLI CCNNNNNNNGG 3 cut(s) 201, 258, 794
BseMI GCAATG 3 cut(s) 542, 715, 861
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 855, 983
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 2 cut(s) 302, 584
BseYI CCCAGC 1 cut(s) 667
Bsh1236I CGCG 2 cut(s) 50, 818
BshFI GGCC 5 cut(s) 13, 105, 121, 514, 615
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 5 cut(s) 15, 39, 66, 252, 435
BslI CCNNNNNNNGG 3 cut(s) 201, 258, 794
BsmAI GTCTC 2 cut(s) 12, 380
BsmI GAATGC 2 cut(s) 605, 811
BsnI GGCC 5 cut(s) 13, 105, 121, 514, 615
Bso31I GGTCTC 1 cut(s) 380
Bsp1286I GDGCHC 1 cut(s) 28
Bsp143I GATC 1 cut(s) 1065
BspACI CCGC 4 cut(s) 48, 106, 536, 1163
BspANI GGCC 5 cut(s) 13, 105, 121, 514, 615
BspCNI CTCAG 1 cut(s) 97
BspFNI CGCG 2 cut(s) 50, 818
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 491
BspPI GGATC 1 cut(s) 1073
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 3 cut(s) 542, 715, 861
BsrI ACTGG 2 cut(s) 855, 983
BssECI CCNNGG 4 cut(s) 100, 113, 160, 509
BssMI GATC 1 cut(s) 1065
BssNAI GTATAC 1 cut(s) 340
BssNI GRCGYC 1 cut(s) 145
BssT1I CCWWGG 4 cut(s) 100, 113, 160, 509
Bst1107I GTATAC 1 cut(s) 340
Bst4CI ACNGT 4 cut(s) 337, 827, 926, 1136
BstACI GRCGYC 1 cut(s) 145
BstAPI GCANNNNNTGC 2 cut(s) 356, 701
BstC8I GCNNGC 2 cut(s) 282, 321
BstDEI CTNAG 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 792
BstF5I GGATG 2 cut(s) 109, 834
BstFNI CGCG 2 cut(s) 50, 818
BstKTI GATC 1 cut(s) 1068
BstMAI GTCTC 2 cut(s) 12, 380
BstMBI GATC 1 cut(s) 1065
BstMWI GCNNNNNNNGC 8 cut(s) 105, 290, 356, 461, 544, 581, 701, 815
BstPAI GACNNNNGTC 1 cut(s) 93
BstSCI CCNGG 2 cut(s) 13, 250
BstUI CGCG 2 cut(s) 50, 818
BstV1I GCAGC 2 cut(s) 302, 584
BstX2I RGATCY 1 cut(s) 1065
BstYI RGATCY 1 cut(s) 1065
BstZ17I GTATAC 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 84
BsuI GTATCC 1 cut(s) 792
BsuRI GGCC 5 cut(s) 13, 105, 121, 514, 615
BtsCI GGATG 2 cut(s) 109, 834
BtsIMutI CAGTG 2 cut(s) 931, 1141
Cac8I GCNNGC 2 cut(s) 282, 321
CaiI CAGNNNCTG 1 cut(s) 356
CciI TCATGA 1 cut(s) 829
Cfr13I GGNCC 2 cut(s) 12, 490
Csp6I GTAC 3 cut(s) 262, 677, 1038
CviAII CATG 7 cut(s) 239, 391, 674, 682, 722, 830, 1009
CviQI GTAC 3 cut(s) 262, 677, 1038
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 1067
DpnII GATC 1 cut(s) 1065
DraIII CACNNNGTG 1 cut(s) 219
DrdI GACNNNNNNGTC 1 cut(s) 144
DseDI GACNNNNNNGTC 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 4 cut(s) 100, 113, 160, 509
Eco24I GRGCYC 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 380
Eco47I GGWCC 1 cut(s) 490
Eco53kI GAGCTC 1 cut(s) 26
Eco81I CCTNAGG 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 792
EcoO109I RGGNCCY 1 cut(s) 490
EcoT14I CCWWGG 4 cut(s) 100, 113, 160, 509
EcoT22I ATGCAT 1 cut(s) 811
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 4 cut(s) 100, 113, 160, 509
FaeI CATG 7 cut(s) 242, 394, 677, 685, 725, 833, 1012
FalI AAGNNNNNCTT 2 cut(s) 1027, 1059
FatI CATG 7 cut(s) 238, 390, 673, 681, 721, 829, 1008
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 5 cut(s) 48, 106, 291, 573, 1163
FokI GGATG 2 cut(s) 96, 821
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 5 cut(s) 48, 106, 291, 573, 1163
FspBI CTAG 6 cut(s) 101, 161, 629, 869, 948, 1167
GluI GCNGC 5 cut(s) 48, 106, 291, 573, 1163
GsaI CCCAGC 1 cut(s) 671
GsuI CTGGAG 1 cut(s) 813
HaeIII GGCC 5 cut(s) 13, 105, 121, 514, 615
HapII CCGG 5 cut(s) 15, 39, 66, 252, 435
Hin1I GRCGYC 1 cut(s) 145
Hin1II CATG 7 cut(s) 242, 394, 677, 685, 725, 833, 1012
HincII GTYRAC 1 cut(s) 1132
HindII GTYRAC 1 cut(s) 1132
HinfI GANTC 5 cut(s) 35, 138, 370, 409, 634
HpaII CCGG 5 cut(s) 15, 39, 66, 252, 435
Hpy166II GTNNAC 5 cut(s) 61, 137, 340, 1132, 1139
Hpy188I TCNGA 3 cut(s) 143, 611, 900
Hpy188III TCNNGA 6 cut(s) 32, 86, 406, 629, 792, 830
Hpy8I GTNNAC 5 cut(s) 61, 137, 340, 1132, 1139
Hpy99I CGWCG 3 cut(s) 132, 147, 150
HpyAV CCTTC 3 cut(s) 798, 949, 989
HpyCH4III ACNGT 4 cut(s) 337, 827, 926, 1136
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 5 cut(s) 359, 572, 605, 695, 809
HpyF10VI GCNNNNNNNGC 8 cut(s) 105, 290, 356, 461, 544, 581, 701, 815
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 1 cut(s) 145
Hsp92I GRCGYC 1 cut(s) 145
Hsp92II CATG 7 cut(s) 242, 394, 677, 685, 725, 833, 1012
Kzo9I GATC 1 cut(s) 1065
LmnI GCTCC 2 cut(s) 277, 452
Lsp1109I GCAGC 2 cut(s) 302, 584
MaeI CTAG 6 cut(s) 101, 161, 629, 869, 948, 1167
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 2 cut(s) 457, 985
MalI GATC 1 cut(s) 1067
MboI GATC 1 cut(s) 1065
MboII GAAGA 3 cut(s) 404, 704, 1147
MfeI CAATTG 3 cut(s) 880, 1059, 1107
MflI RGATCY 1 cut(s) 1065
MhlI GDGCHC 1 cut(s) 28
MluCI AATT 8 cut(s) 424, 522, 619, 880, 1047, 1059, 1107, 1145
MluI ACGCGT 1 cut(s) 816
MlyI GAGTC 1 cut(s) 132
MmeI TCCRAC 1 cut(s) 166
Mph1103I ATGCAT 1 cut(s) 811
MroXI GAANNNNTTC 4 cut(s) 177, 246, 537, 1154
MseI TTAA 4 cut(s) 185, 660, 1046, 1143
MslI CAYNNNNRTG 3 cut(s) 720, 726, 828
MspI CCGG 5 cut(s) 15, 39, 66, 252, 435
MspR9I CCNGG 2 cut(s) 15, 252
MunI CAATTG 3 cut(s) 880, 1059, 1107
Mva1269I GAATGC 2 cut(s) 605, 811
MvnI CGCG 2 cut(s) 50, 818
MwoI GCNNNNNNNGC 8 cut(s) 105, 290, 356, 461, 544, 581, 701, 815
NciI CCSGG 2 cut(s) 15, 252
NdeII GATC 1 cut(s) 1065
NlaIII CATG 7 cut(s) 242, 394, 677, 685, 725, 833, 1012
NlaIV GGNNCC 1 cut(s) 491
NmuCI GTSAC 2 cut(s) 457, 985
NsiI ATGCAT 1 cut(s) 811
PagI TCATGA 1 cut(s) 829
PctI GAATGC 2 cut(s) 605, 811
PdmI GAANNNNTTC 4 cut(s) 177, 246, 537, 1154
PfeI GAWTC 4 cut(s) 35, 370, 409, 634
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 5 cut(s) 49, 107, 292, 574, 1164
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PpuMI RGGWCCY 1 cut(s) 490
PshAI GACNNNNGTC 1 cut(s) 93
Psp124BI GAGCTC 1 cut(s) 28
Psp5II RGGWCCY 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 667
PspN4I GGNNCC 1 cut(s) 491
PspPI GGNCC 2 cut(s) 12, 490
PspPPI RGGWCCY 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 1065
RsaI GTAC 3 cut(s) 263, 678, 1039
RsaNI GTAC 3 cut(s) 262, 677, 1038
RseI CAYNNNNRTG 3 cut(s) 720, 726, 828
SacI GAGCTC 1 cut(s) 28
SaqAI TTAA 4 cut(s) 185, 660, 1046, 1143
SatI GCNGC 5 cut(s) 48, 106, 291, 573, 1163
Sau3AI GATC 1 cut(s) 1065
Sau96I GGNCC 2 cut(s) 12, 490
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 2 cut(s) 15, 252
SduI GDGCHC 1 cut(s) 28
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 3 cut(s) 720, 726, 828
SmlI CTYRAG 1 cut(s) 285
SmoI CTYRAG 1 cut(s) 285
Sse9I AATT 8 cut(s) 424, 522, 619, 880, 1047, 1059, 1107, 1145
SsiI CCGC 4 cut(s) 48, 106, 536, 1163
SspMI CTAG 6 cut(s) 101, 161, 629, 869, 948, 1167
SstI GAGCTC 1 cut(s) 28
StyD4I CCNGG 2 cut(s) 13, 250
StyI CCWWGG 4 cut(s) 100, 113, 160, 509
TaaI ACNGT 4 cut(s) 337, 827, 926, 1136
TaiI ACGT 1 cut(s) 148
TaqI TCGA 2 cut(s) 33, 921
TasI AATT 8 cut(s) 424, 522, 619, 880, 1047, 1059, 1107, 1145
TatI WGTACW 1 cut(s) 1037
TauI GCSGC 3 cut(s) 50, 108, 1165
TfiI GAWTC 4 cut(s) 35, 370, 409, 634
Tru1I TTAA 4 cut(s) 185, 660, 1046, 1143
Tru9I TTAA 4 cut(s) 185, 660, 1046, 1143
TscAI CASTG 2 cut(s) 931, 1141
TseFI GTSAC 2 cut(s) 457, 985
TseI GCWGC 2 cut(s) 290, 572
Tsp45I GTSAC 2 cut(s) 457, 985
TspDTI ATGAA 7 cut(s) 141, 255, 407, 432, 546, 704, 997
TspRI CASTG 2 cut(s) 931, 1141
VpaK11BI GGWCC 1 cut(s) 490
XagI CCTNNNNNAGG 1 cut(s) 792
XbaI TCTAGA 1 cut(s) 628
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XmaJI CCTAGG 2 cut(s) 100, 160
XmiI GTMKAC 1 cut(s) 339
XmnI GAANNNNTTC 4 cut(s) 177, 246, 537, 1154
XspI CTAG 6 cut(s) 101, 161, 629, 869, 948, 1167
ZraI GACGTC 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.