FvH4_2g37660

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004402
Physical Location & Seq
Forward (+)
27372860 .. 27377651
4792 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37660.t1

Sequence Viewer

Length: 1557 bp
ATGAGTAGTGAGGTGACGGAAGTGAAAGTAAACTCAATGTCGTCGGCAAGGAGGGCAGCAGATTATTCAGAAATCGAAACTCCGGCAGCCAATATGGCAAGCCCGGTTGGTCCGCCAAAAACACCTAAGAGCCCTTTTGCTTCGCGTTTTATGAGTACTCCTCTTGCCAGCCCCATGAAAAAGGCGATTGTAAGCATGCAGGGCTACTTGGAAGAAGTTGGACACTTCACCAAGCTTGATCCTCAAGACGCCTGGCTACCCATCACCGAGTCGAGAAATGGTAATGCCTACTACTCTGCGTTTCACACTCTGAGTTCCGGTATCGGAGTTCAAGCCCTCCTTCTTCCGCTGTCTTTCACTGTTCTTGGTTGGGTATGGGGAATCATATGCCTCTCAGTGGCTTTCATGTGGCAGTTATATACTCTCTGGTTACTGATACAGCTTCATGAATCACAATCTGGGATGCGCTACAGTCGATACCTCCGCCTTTCCATGGCTGCTTTTGGTGAGAAACTAGGGAAGCTGCTTTCGCTGTTTCCGATCATGTACCTCTCCGGTGGCACGTGTGTGACCCTGATCATGATCGGTGGTGGAACCATGAAGATCTTTTACCAAATCGTTAGCGGTAACAATACAAGTCCTTTGACCATCGTGGAGTGGTACTTGGTGTTCACCAGCACGGCCACTGTTCTAGCTCAACTCCCTAACCTCAACTCCATTGCCGGAGTTTCACTTATCGGAGCGATCACTGCTGTAACTTACTGCACACTCATTTGGGTTGTTTCCATTACCAGGGATAGGCCAGCGGGTGTCAACTATGACTATCCAGCACTTCCTCCCCAAGCAAAATCTGATCCAGCTGCTTCGGTTTTTAGTATTCTCAATGCTATTGGGATAACTGTTTTTGCTTTTAGAGGCCACAATCTTGTGCTCGAAATACAGGGGACTATGCCTTCAAGCATAAAGAGCCCGTCGCGTTTGCCAATGTGGAAAGGGGTGAAGTTTGCATATCTCATCATAGCAATGTGCTTGTTTCCCTTAGCAATCGGAGGCTATTGGGCTTACGGGAATTTGATACCATCAAACGGAGGAATGCTAAATGCTCTGAACAACTACCATGGACACGATACGTCAAAATTTGTGCTGGGTTTTACAAGCCTGCTGGTTGTGATCAACTGCCTAACCTCGTTTCAGATATATGCAATGCCGGTGTTCGACAATCTGGAATTCAGATACACTAGCAGCATGAACAAACCCTGTCCACGGTGGCTCCGCGCAGGGTTTAGGATTTTGTTTGGCTGTTTTGCCTGTTTTATTGCAGTGGCACTACCATTCTTGCCAAGCTTGGCAGGGTTGATCGGAGGAGTTGCTGTACCTATCACATTAGCATATCCATGTTTCATGTGGATACTCATCACAAAACCTCCTAAACATATTGCAATATTCAATTGGGTACTGGGATCCTTGGGGATAGTCCTTAGTATCTTAATTGTCACTGGAGCAATTTGGAATATAGTGACCATCGGAATAGAAGTTCACTTCTTCAAGCCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

519

Amino Acids

56.68

Weight (kDa)

9.27

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 93 - 505 9.4e-55 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 145, 976, 1275
AciI CCGC 6 cut(s) 113, 347, 484, 624, 806, 1273
AclWI GGATC 4 cut(s) 233, 848, 1455, 1468
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 3 cut(s) 1069, 1136, 1226
AcvI CACGTG 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 5 cut(s) 157, 548, 662, 1374, 1455
AfiI CCNNNNNNNGG 6 cut(s) 397, 493, 792, 798, 1085, 1263
AflIII ACRYGT 1 cut(s) 563
AgsI TTSAA 4 cut(s) 332, 957, 1447, 1546
AjnI CCWGG 2 cut(s) 251, 791
AleI CACNNNNGTG 1 cut(s) 566
AloI GAACNNNNNNTCC 2 cut(s) 1518, 1550
AluBI AGCT 6 cut(s) 235, 442, 523, 695, 860, 1344
AluI AGCT 6 cut(s) 235, 442, 523, 695, 860, 1344
Alw21I GWGCWC 1 cut(s) 933
AlwI GGATC 4 cut(s) 233, 848, 1455, 1468
AoxI GGCC 3 cut(s) 681, 800, 916
ApeKI GCWGC 6 cut(s) 56, 86, 497, 523, 860, 1242
ApoI RAATTY 3 cut(s) 1069, 1136, 1226
AspLEI GCGC 2 cut(s) 468, 1277
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 1 cut(s) 104
AsuHPI GGTGA 6 cut(s) 25, 220, 256, 518, 664, 1009
AvaII GGWCC 1 cut(s) 110
BamHI GGATCC 1 cut(s) 1460
BanII GRGCYC 2 cut(s) 134, 971
BarI GAAGNNNNNNTAC 2 cut(s) 593, 625
BbrPI CACGTG 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 933
BbvI GCAGC 6 cut(s) 68, 98, 484, 510, 847, 1254
BccI CCATC 4 cut(s) 269, 656, 1087, 1529
BceAI ACGGC 1 cut(s) 696
BciT130I CCWGG 2 cut(s) 253, 793
BciVI GTATCC 1 cut(s) 1401
BclI TGATCA 2 cut(s) 576, 1170
BcnI CCSGG 1 cut(s) 104
BfaI CTAG 3 cut(s) 515, 692, 1239
BfmI CTRYAG 1 cut(s) 469
BfuI GTATCC 1 cut(s) 1401
BglI GCCNNNNNGGC 1 cut(s) 95
BglII AGATCT 1 cut(s) 603
BisI GCNGC 6 cut(s) 57, 87, 498, 524, 861, 1243
BlsI GCNGC 6 cut(s) 58, 88, 499, 525, 862, 1244
BmcAI AGTACT 1 cut(s) 157
Bme1390I CCNGG 3 cut(s) 104, 253, 793
Bme18I GGWCC 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 3 cut(s) 595, 1271, 1462
BmrFI CCNGG 3 cut(s) 104, 253, 793
BmrI ACTGGG 1 cut(s) 1466
BmsI GCATC 1 cut(s) 453
BmuI ACTGGG 1 cut(s) 1466
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BpmI CTGGAG 1 cut(s) 1518
Bpu10I CCTNAGC 1 cut(s) 1039
BpuEI CTTGAG 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 104
BsaAI YACGTR 1 cut(s) 564
BsaBI GATNNNNATC 2 cut(s) 581, 1412
BsaHI GRCGYC 1 cut(s) 249
BsaJI CCNNGG 5 cut(s) 492, 792, 1117, 1262, 1464
BsaWI WCCGGW 2 cut(s) 317, 554
BsaXI ACNNNNNCTCC 8 cut(s) 698, 728, 1254, 1284, 1356, 1386, 1408, 1438
Bsc4I CCNNNNNNNGG 6 cut(s) 397, 493, 792, 798, 1085, 1263
Bse118I RCCGGY 1 cut(s) 1207
Bse1I ACTGG 2 cut(s) 1461, 1501
Bse3DI GCAATG 3 cut(s) 717, 1029, 1209
Bse8I GATNNNNATC 2 cut(s) 581, 1412
BseBI CCWGG 2 cut(s) 253, 793
BseDI CCNNGG 5 cut(s) 492, 792, 1117, 1262, 1464
BseGI GGATG 1 cut(s) 468
BseJI GATNNNNATC 2 cut(s) 581, 1412
BseLI CCNNNNNNNGG 6 cut(s) 397, 493, 792, 798, 1085, 1263
BseMI GCAATG 3 cut(s) 717, 1029, 1209
BseMII CTCAG 2 cut(s) 302, 408
BseNI ACTGG 2 cut(s) 1461, 1501
BseRI GAGGAG 2 cut(s) 150, 1377
BseXI GCAGC 6 cut(s) 68, 98, 484, 510, 847, 1254
BseYI CCCAGC 1 cut(s) 1144
BsgI GTGCAG 1 cut(s) 748
Bsh1236I CGCG 3 cut(s) 145, 976, 1275
BshFI GGCC 3 cut(s) 683, 802, 918
BsiHKAI GWGCWC 1 cut(s) 933
BsiSI CCGG 6 cut(s) 83, 104, 318, 555, 723, 1208
BslFI GGGAC 1 cut(s) 958
BslI CCNNNNNNNGG 6 cut(s) 397, 493, 792, 798, 1085, 1263
BsmFI GGGAC 1 cut(s) 958
BsmI GAATGC 1 cut(s) 1098
BsnI GGCC 3 cut(s) 683, 802, 918
Bsp1286I GDGCHC 3 cut(s) 134, 933, 971
Bsp19I CCATGG 2 cut(s) 492, 1117
BspACI CCGC 6 cut(s) 113, 347, 484, 624, 806, 1273
BspANI GGCC 3 cut(s) 683, 802, 918
BspCNI CTCAG 2 cut(s) 303, 407
BspFNI CGCG 3 cut(s) 145, 976, 1275
BspHI TCATGA 2 cut(s) 445, 579
BspLI GGNNCC 3 cut(s) 595, 1271, 1462
BspPI GGATC 4 cut(s) 233, 848, 1455, 1468
BsrDI GCAATG 3 cut(s) 717, 1029, 1209
BsrFI RCCGGY 1 cut(s) 1207
BsrI ACTGG 2 cut(s) 1461, 1501
BssAI RCCGGY 1 cut(s) 1207
BssECI CCNNGG 5 cut(s) 492, 792, 1117, 1262, 1464
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 3 cut(s) 492, 1117, 1464
Bst2UI CCWGG 2 cut(s) 253, 793
Bst4CI ACNGT 5 cut(s) 361, 473, 688, 901, 1266
BstACI GRCGYC 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 564
BstC8I GCNNGC 5 cut(s) 100, 169, 197, 804, 1160
BstDEI CTNAG 5 cut(s) 126, 311, 394, 1039, 1478
BstDSI CCRYGG 3 cut(s) 492, 1117, 1262
BstF5I GGATG 1 cut(s) 468
BstFNI CGCG 3 cut(s) 145, 976, 1275
BstHHI GCGC 2 cut(s) 468, 1277
BstMWI GCNNNNNNNGC 6 cut(s) 53, 95, 201, 529, 749, 966
BstNI CCWGG 2 cut(s) 253, 793
BstNSI RCATGY 1 cut(s) 199
BstSCI CCNGG 3 cut(s) 102, 251, 791
BstSFI CTRYAG 1 cut(s) 469
BstUI CGCG 3 cut(s) 145, 976, 1275
BstV1I GCAGC 6 cut(s) 68, 98, 484, 510, 847, 1254
BstX2I RGATCY 2 cut(s) 603, 1460
BstYI RGATCY 2 cut(s) 603, 1460
BsuI GTATCC 1 cut(s) 1401
BsuRI GGCC 3 cut(s) 683, 802, 918
BtgI CCRYGG 3 cut(s) 492, 1117, 1262
BtsCI GGATG 1 cut(s) 468
BtsI GCAGTG 2 cut(s) 747, 1326
BtsIMutI CAGTG 6 cut(s) 357, 402, 684, 747, 1326, 1494
Cac8I GCNNGC 5 cut(s) 100, 169, 197, 804, 1160
CciI TCATGA 2 cut(s) 445, 579
CfoI GCGC 2 cut(s) 468, 1277
Cfr10I RCCGGY 1 cut(s) 1207
Cfr13I GGNCC 1 cut(s) 110
CseI GACGC 1 cut(s) 257
Csp6I GTAC 5 cut(s) 156, 547, 661, 1373, 1454
CviQI GTAC 5 cut(s) 156, 547, 661, 1373, 1454
DdeI CTNAG 5 cut(s) 126, 311, 394, 1039, 1478
EaeI YGGCCR 1 cut(s) 681
EciI GGCGGA 2 cut(s) 102, 473
Eco130I CCWWGG 3 cut(s) 492, 1117, 1464
Eco24I GRGCYC 2 cut(s) 134, 971
Eco47I GGWCC 1 cut(s) 110
Eco72I CACGTG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 1226
EcoRII CCWGG 2 cut(s) 251, 791
EcoT14I CCWWGG 3 cut(s) 492, 1117, 1464
EcoT38I GRGCYC 2 cut(s) 134, 971
ErhI CCWWGG 3 cut(s) 492, 1117, 1464
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FaqI GGGAC 1 cut(s) 958
FauI CCCGC 1 cut(s) 799
FauNDI CATATG 1 cut(s) 386
FbaI TGATCA 2 cut(s) 576, 1170
Fnu4HI GCNGC 6 cut(s) 57, 87, 498, 524, 861, 1243
FokI GGATG 1 cut(s) 475
FriOI GRGCYC 2 cut(s) 134, 971
Fsp4HI GCNGC 6 cut(s) 57, 87, 498, 524, 861, 1243
FspBI CTAG 3 cut(s) 515, 692, 1239
GlaI GCGC 2 cut(s) 467, 1276
GluI GCNGC 6 cut(s) 57, 87, 498, 524, 861, 1243
GsaI CCCAGC 1 cut(s) 1148
GsuI CTGGAG 1 cut(s) 1518
HaeIII GGCC 3 cut(s) 683, 802, 918
HapII CCGG 6 cut(s) 83, 104, 318, 555, 723, 1208
HgaI GACGC 1 cut(s) 257
HhaI GCGC 2 cut(s) 468, 1277
Hin1I GRCGYC 1 cut(s) 249
Hin6I GCGC 2 cut(s) 466, 1275
HinP1I GCGC 2 cut(s) 466, 1275
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HindIII AAGCTT 2 cut(s) 233, 1342
HinfI GANTC 3 cut(s) 269, 381, 449
HpaII CCGG 6 cut(s) 83, 104, 318, 555, 723, 1208
HphI GGTGA 6 cut(s) 25, 220, 256, 518, 664, 1009
Hpy166II GTNNAC 5 cut(s) 31, 672, 814, 1262, 1537
Hpy188III TCNNGA 5 cut(s) 245, 273, 446, 580, 1223
Hpy8I GTNNAC 5 cut(s) 31, 672, 814, 1262, 1537
Hpy99I CGWCG 2 cut(s) 46, 976
HpyAV CCTTC 2 cut(s) 350, 963
HpyCH4III ACNGT 5 cut(s) 361, 473, 688, 901, 1266
HpyCH4IV ACGT 2 cut(s) 563, 1130
HpyCH4V TGCA 6 cut(s) 199, 765, 1007, 1202, 1319, 1439
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 95, 201, 529, 749, 966
HpyF3I CTNAG 5 cut(s) 126, 311, 394, 1039, 1478
HpySE526I ACGT 2 cut(s) 563, 1130
Hsp92I GRCGYC 1 cut(s) 249
HspAI GCGC 2 cut(s) 466, 1275
Ksp22I TGATCA 2 cut(s) 576, 1170
LmnI GCTCC 3 cut(s) 740, 1275, 1499
Lsp1109I GCAGC 6 cut(s) 68, 98, 484, 510, 847, 1254
LweI GCATC 1 cut(s) 453
MaeI CTAG 3 cut(s) 515, 692, 1239
MaeII ACGT 2 cut(s) 563, 1130
MaeIII GTNAC 7 cut(s) 13, 429, 568, 626, 754, 1492, 1516
MboII GAAGA 4 cut(s) 224, 335, 613, 1534
MfeI CAATTG 1 cut(s) 1447
MflI RGATCY 2 cut(s) 603, 1460
MhlI GDGCHC 3 cut(s) 134, 933, 971
MluCI AATT 6 cut(s) 1069, 1136, 1226, 1447, 1488, 1503
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 199
MseI TTAA 1 cut(s) 1487
MslI CAYNNNNRTG 3 cut(s) 566, 1022, 1393
MspA1I CMGCKG 3 cut(s) 349, 806, 860
MspI CCGG 6 cut(s) 83, 104, 318, 555, 723, 1208
MspR9I CCNGG 3 cut(s) 104, 253, 793
MunI CAATTG 1 cut(s) 1447
Mva1269I GAATGC 1 cut(s) 1098
MvaI CCWGG 2 cut(s) 253, 793
MvnI CGCG 3 cut(s) 145, 976, 1275
MwoI GCNNNNNNNGC 6 cut(s) 53, 95, 201, 529, 749, 966
NciI CCSGG 1 cut(s) 104
NcoI CCATGG 2 cut(s) 492, 1117
NdeI CATATG 1 cut(s) 386
NlaIV GGNNCC 3 cut(s) 595, 1271, 1462
NmuCI GTSAC 4 cut(s) 13, 568, 1492, 1516
NspI RCATGY 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 566
PaeI GCATGC 1 cut(s) 199
PagI TCATGA 2 cut(s) 445, 579
PcsI WCGNNNNNNNCGW 1 cut(s) 536
PctI GAATGC 1 cut(s) 1098
PfeI GAWTC 2 cut(s) 381, 449
PkrI GCNGC 6 cut(s) 58, 88, 499, 525, 862, 1244
PleI GAGTC 1 cut(s) 277
PmaCI CACGTG 1 cut(s) 564
PmlI CACGTG 1 cut(s) 564
PpsI GAGTC 1 cut(s) 277
Ppu21I YACGTR 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 251, 791
PspCI CACGTG 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 1144
PspGI CCWGG 2 cut(s) 251, 791
PspN4I GGNNCC 3 cut(s) 595, 1271, 1462
PspPI GGNCC 1 cut(s) 110
PsrI GAACNNNNNNTAC 2 cut(s) 653, 685
PsuI RGATCY 2 cut(s) 603, 1460
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 5 cut(s) 157, 548, 662, 1374, 1455
RsaNI GTAC 5 cut(s) 156, 547, 661, 1373, 1454
RseI CAYNNNNRTG 3 cut(s) 566, 1022, 1393
SaqAI TTAA 1 cut(s) 1487
SatI GCNGC 6 cut(s) 57, 87, 498, 524, 861, 1243
Sau96I GGNCC 1 cut(s) 110
ScaI AGTACT 1 cut(s) 157
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 3 cut(s) 104, 253, 793
SduI GDGCHC 3 cut(s) 134, 933, 971
SfaNI GCATC 1 cut(s) 453
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 110
SmiMI CAYNNNNRTG 3 cut(s) 566, 1022, 1393
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SphI GCATGC 1 cut(s) 199
Sse9I AATT 6 cut(s) 1069, 1136, 1226, 1447, 1488, 1503
SsiI CCGC 6 cut(s) 113, 347, 484, 624, 806, 1273
SspI AATATT 1 cut(s) 1443
SspMI CTAG 3 cut(s) 515, 692, 1239
StyD4I CCNGG 3 cut(s) 102, 251, 791
StyI CCWWGG 3 cut(s) 492, 1117, 1464
TaaI ACNGT 5 cut(s) 361, 473, 688, 901, 1266
TaiI ACGT 2 cut(s) 566, 1133
TaqI TCGA 5 cut(s) 75, 272, 475, 933, 1215
TasI AATT 6 cut(s) 1069, 1136, 1226, 1447, 1488, 1503
TatI WGTACW 1 cut(s) 155
TfiI GAWTC 2 cut(s) 381, 449
Tru1I TTAA 1 cut(s) 1487
Tru9I TTAA 1 cut(s) 1487
TscAI CASTG 6 cut(s) 364, 402, 691, 754, 1326, 1501
TseFI GTSAC 4 cut(s) 13, 568, 1492, 1516
TseI GCWGC 6 cut(s) 56, 86, 497, 523, 860, 1242
Tsp45I GTSAC 4 cut(s) 13, 568, 1492, 1516
TspDTI ATGAA 7 cut(s) 191, 394, 434, 462, 614, 1262, 1390
TspGWI ACGGA 2 cut(s) 32, 1101
TspRI CASTG 6 cut(s) 364, 402, 691, 754, 1326, 1501
VpaK11BI GGWCC 1 cut(s) 110
XapI RAATTY 3 cut(s) 1069, 1136, 1226
XceI RCATGY 1 cut(s) 199
XcmI CCANNNNNNNNNTGG 1 cut(s) 1401
XspI CTAG 3 cut(s) 515, 692, 1239
ZrmI AGTACT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.