Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010393

Physical Location & Seq

Genomic Coordinates Reverse (-)

18591194 .. 18598569

Gene Structure 7376 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G50200.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGAGATTAGTGAAGGCAGCTTCTCTTCTGATTTCTTCAACCAAACCTCCGTCTAGGGTTTTTTACTCTTCCCATCTCCGCCGCCCTTTTTTCTCTCACTTCCGTTTTTCTTCCTCTTCGTCGACGTCTTCCTCCGTCGCAGTCATGCCGGGTTCCGAACCATCGGAGACTCAGTGGCCAGCAAAGAGAGTCCGGGATACTTATGTCGATTTCTTCAGAGGCAAAGGCCACAAGTTCTGGCCATCAAGCCCCGTTGTTCCTCATAATGATCCTACTCTTCTCTTCGCTAACGCTGGAATGAACCAGTATAAGCCAATATTTTTGGGTACGGCCGATCCAAATACTGAGCTTAGCAAGCTATCTCGGGCATGTAACACCCAAAAGTGTATTCGTGCTGGTGGAAAGCATAATGATTTGGATGATGTCGGCAAAGATACGTACCATCACACCTTCTTTGAAATGCTTGGTAACTGGTCCTTTGGAGATTATTTCAAGAAGGAAGCTATCGAATGGGCTTGGGAGCTCTTAACCAAGGTCTATGGGCTACCGACTGATCGAATCTACGCTACATATTTTGGCGGTGATGAGAAAGCTGGCCTTCAACCTGATAATGAGGCTAGGGATATATGGTTGAAGGTTCTTCCATCTGGACGTGTGCTGCCATTTGGTTGCAAAGACAATTTCTGGGAAATGGGCGACACAGGTCCGTGTGGACCTTGTACTGAAATACATTATGATCGCATTGGTAACCGTGATGCTGCATCCTTAGTTAACAATGACGATCCAACATGTTTAGAAATATGGAATCTTGTCTTTATTCAGTTCAACCGAGAAAGTGATGGCTCGCTAAAACCTCTGCCTGCTAAGCATGTTGATACCGGGATGGGCTTCGAGAGGTTGACTTCTGTTCTTCAAAACAAAATGAGCAACTATGATACAGATGTGTTCATGCCTATCTTTGATGACATCCAAAAGGCAACGGGAGCTAGGCCATATTCTGGAAAAGTTGGTCCGGAAGATGTTGACAGAGTTGACATGGCATATAGAGTGGTTGCTGATCATATTAGAACCCTATCATTTGCAATTGCGGATGGCTCTCGTCCTGGTAATGAGGGCCGTGAGTATGTTCTGCGGCGTATTCTTCGTCGAGCGGTTCGCTATGGAAAGGAGATCCTAAAAGCTGAAGAAGGATTTTTCAACGGGCTTGTAAGTTCTGTCATTCGGGTGATGGGCGATGTCTTTACAGAGTTGAAGGAACATGAGAAAAAGATCACAGATATAATTAAAGAAGAGGAAGCAAGCTTTTGCAAGACCTTGGCAAAGGGAATTGAGAAATTTCGGAAAGCTGGACAGGCGGTTCAGGGGAATACACTGAGTGGAGATGATGCTTTTATCTTATGGGATACATATGGTTTCCCGTTAGATTTAACTCAGTTGATGGCTGAAGAAAGAGGGTTACTGGTTGATGTTGATGGTTTCAACAAAGCCATGGAAGAGGCGAGGGAAAGATCGAGAAGTGCCCAGAATAAGCAAGCTGGTGGTGCCATTGTTATGGATGCTGATGCTACATCAACATTGCACAAGGCTGGTGTTTCAGCAACAGATGATTCTTTTAAATACATTTGGTTCCAGGACCACGAGAGTGAACTAAAGGCAATCTATACTGGCTCTACTTTCCTAGAAAGTTCAGCTGCTAGTGATAACGTGGGACTAGTATTGGGGTCTACAAGCTTCTATGCTGAGCAGGGTGGTCAGATTTTTGACACTGGACTTATAGAAGGCTCGTTTGGGACATTCAATGTATGCAATGTACAAATATTTGGGGGCTTTGTTCTTCACATTGGTTATCTCTCTAAAGAAACTGGAGAGGTATCTGTGGGTGATAAAGTGATTTGCAAGGTTGACTATGAGAGGCGTAAACTCATCGCTCCTAATCATACTTGCACACATATGTTGAATTATGCCCTGAAGGAAGTGCTCGGGGATCATATTGATCAGAAGGGATCAATTGTTCTTCCTGAAAAACTGCGATTTGATTTTTCTCATGGCAAGCCGGTTGATCCTGAAGATTTGAGAAGGATCGAATCCATAGTGAATAAACAGATCAAGGATGAATTAGATGTGTTTTCCAAGGAAGCTGTGCTTTCTGAAGCCAAGCGCATCAAAGGTCTAAGAGCAGTGTTTGGAGAAGTCTACCCCGATCCCGTCAGAGTGGTGTCAATTGGGAGGAAGGTTGAGGATCTCTTGGCTGATCCTGAAAACAATGAATGGTCATTGCTTTCTTCCGAGTTTTGTGGAGGAACCCACATAACAAACACCCGCGAAGCCAAAGCATTTGCTCTTCTATCGGAGGAGGGAATTGCTAAAGGTATTCGTAGGGTAACTGCTGTGACTACTGAATGTGCTTTTGATGCATTGAATGCGGCGTCCTTACTTGAAAGAGAAGTAGAGGATGCCTCCAGAGCGGAGGGAAGTGCATTGGAAAAGAAAGTTTCTGCTTTGAAAAGCCGAGTAGATGCAGCAATTATCCCAGCAGCTAAAAAGGCAGATATTAGGACTAAGATTGCTTCGCTTCAGAATGAAGTAAGAAAAGCTCAGAAGAAAATAGCGGAACAAAACCTGAAAAAATCTGTCAAATTAGCAACAGAGGCAGCTGAGTCCGCAGCATCAGATGGGAAGACTTTCTGCATAATCCAGCTGGATGTGGGTCTTGATGCAGCAGCTGTGCGAGAGGCCGTTTCAAAAGTCATGGAAAAGAAGGGTATGTCGATAATGGTGTTCAGCACAGATGAAAGCACAAACAAGGCGGTTGTGTGTGCAGGAGTGCCAGAAAAATCAGACCAGTTTAAGCCGTTAGACGTCACTGAATGGTTGACAACTGCATTGGGTCCTCTAAAAGGAAGGTGCGGGAAAGGGAAAGGTGGTCTTGCATCAGGGCAGGGAACGGATGCTTCTCAAGTGCAGGCGGCTTTGGATATGGCTTCATCATTTGCATCAATGAAGCTCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1003

Amino Acids

110.49

Weight (kDa)

6.05

Isoelectric Point (pI)

35.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	64 - 638	6e-223	tRNA synthetases class II (A)
tRNA_SAD	PF07973	736 - 794	8.7e-13	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	902 - 1006	1.4e-11	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50200 AT1G50200
fragaria_vesca	FvH4_4g36780 FvH4_4g36780
malus_domestica	MD13G1007000.v1.1 MD16G1000800.v1.1
prunus_persica	Prupe.1G345900_v2.0.a1
pyrus_communis	pycom13g00590 pycom16g00460
rosa_chinensis	RchiOBHm_Chr4g0446511
rosa_laevigata	RLG00000005653
rosa_multiflora	Rmu_co8401425.1_g000001 Rmu_sc0005526.1_g000002 Rmu_sc0009884.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386930
rosa_rugosa	Rorug04G0372100 Rorug04G0372200
rosa_samantha	Rh4BG442800 Rh4CG460300 Rh4DG441500
rosa_wichuraiana	Rw4G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	128, 2862
AccB1I	GGYRCC	1 cut(s)	1541
AccB7I	CCANNNNNTGG	1 cut(s)	998
AccBSI	CCGCTC	2 cut(s)	1151, 2465
AccI	GTMKAC	3 cut(s)	122, 1724, 2193
AccII	CGCG	1 cut(s)	2322
AccIII	TCCGGA	1 cut(s)	1012
AcoI	YGGCCR	3 cut(s)	176, 239, 330
AcsI	RAATTY	1 cut(s)	1334
AcuI	CTGAAG	7 cut(s)	199, 1203, 1464, 1988, 2085, 2169, 2558
AcyI	GRCGYC	3 cut(s)	125, 2426, 2859
AdeI	CACNNNGTG	1 cut(s)	1376
AfaI	GTAC	4 cut(s)	328, 440, 721, 1812
AfiI	CCNNNNNNNGG	2 cut(s)	998, 2897
AflIII	ACRYGT	2 cut(s)	652, 788
AhlI	ACTAGT	1 cut(s)	1711
AjiI	CACGTC	1 cut(s)	653
AjnI	CCWGG	2 cut(s)	1102, 1629
AjuI	GAANNNNNNNTTGG	4 cut(s)	372, 404, 1770, 1802
AleI	CACNNNNGTG	1 cut(s)	1641
AloI	GAACNNNNNNTCC	4 cut(s)	1341, 1373, 1995, 2027
Alw21I	GWGCWC	2 cut(s)	525, 1980
Alw26I	GTCTC	1 cut(s)	161
AlwNI	CAGNNNCTG	1 cut(s)	2723
Ama87I	CYCGRG	2 cut(s)	363, 1979
Aor13HI	TCCGGA	1 cut(s)	1012
AoxI	GGCC	8 cut(s)	176, 226, 239, 330, 595, 989, 1114, 2733
ApoI	RAATTY	1 cut(s)	1334
Asp700I	GAANNNNTTC	1 cut(s)	2681
AspLEI	GCGC	1 cut(s)	2160
AspS9I	GGNCC	7 cut(s)	474, 704, 713, 1010, 1114, 1633, 2888
AsuC2I	CCSGG	3 cut(s)	150, 194, 880
AsuHPI	GGTGA	3 cut(s)	593, 1237, 1892
AvaI	CYCGRG	2 cut(s)	363, 1979
AvaII	GGWCC	6 cut(s)	474, 704, 713, 1010, 1633, 2888
BaeGI	GKGCMC	1 cut(s)	1522
BaeI	ACNNNNGTAYC	4 cut(s)	189, 222, 927, 960
BalI	TGGCCA	2 cut(s)	178, 241
BanI	GGYRCC	1 cut(s)	1541
BanII	GRGCYC	1 cut(s)	525
BauI	CACGAG	1 cut(s)	1637
BbsI	GAAGAC	2 cut(s)	120, 2684
Bbv12I	GWGCWC	2 cut(s)	525, 1980
BceAI	ACGGC	4 cut(s)	345, 1101, 2720, 2836
BciT130I	CCWGG	2 cut(s)	1104, 1631
BciVI	GTATCC	2 cut(s)	190, 1396
BclI	TGATCA	2 cut(s)	1057, 1993
BcnI	CCSGG	3 cut(s)	150, 194, 880
BcoDI	GTCTC	1 cut(s)	161
BcuI	ACTAGT	1 cut(s)	1711
BfaI	CTAG	6 cut(s)	54, 618, 987, 1679, 1695, 1712
BfuI	GTATCC	2 cut(s)	190, 1396
BlpI	GCTNAGC	3 cut(s)	350, 864, 1740
Bme1390I	CCNGG	5 cut(s)	150, 194, 880, 1104, 1631
Bme18I	GGWCC	6 cut(s)	474, 704, 713, 1010, 1633, 2888
BmeT110I	CYCGRG	2 cut(s)	363, 1979
BmgBI	CACGTC	1 cut(s)	653
BmgT120I	GGNCC	7 cut(s)	474, 704, 713, 1010, 1114, 1633, 2888
BmiI	GGNNCC	5 cut(s)	154, 1543, 1628, 2302, 2889
BmrFI	CCNGG	5 cut(s)	150, 194, 880, 1104, 1631
BoxI	GACNNNNGTC	1 cut(s)	702
BpiI	GAAGAC	2 cut(s)	120, 2684
BplI	GAGNNNNNCTC	2 cut(s)	2441, 2473
BpmI	CTGGAG	2 cut(s)	1884, 2443
Bpu1102I	GCTNAGC	3 cut(s)	350, 864, 1740
BpuEI	CTTGAG	1 cut(s)	2940
BpuMI	CCSGG	3 cut(s)	150, 194, 880
BsaAI	YACGTR	1 cut(s)	438
BsaHI	GRCGYC	3 cut(s)	125, 2426, 2859
BsaJI	CCNNGG	4 cut(s)	531, 1314, 1488, 2130
BsaWI	WCCGGW	1 cut(s)	1012
BsaXI	ACNNNNNCTCC	4 cut(s)	31, 61, 158, 188
Bsc4I	CCNNNNNNNGG	2 cut(s)	998, 2897
Bse118I	RCCGGY	1 cut(s)	2053
Bse1I	ACTGG	7 cut(s)	304, 476, 1464, 1669, 1771, 1867, 2842
Bse3DI	GCAATG	3 cut(s)	1574, 1813, 2273
BseAI	TCCGGA	1 cut(s)	1012
BseBI	CCWGG	2 cut(s)	1104, 1631
BseDI	CCNNGG	4 cut(s)	531, 1314, 1488, 2130
BseLI	CCNNNNNNNGG	2 cut(s)	998, 2897
BseMI	GCAATG	3 cut(s)	1574, 1813, 2273
BseMII	CTCAG	7 cut(s)	185, 336, 1364, 1445, 1731, 2609, 2646
BseNI	ACTGG	7 cut(s)	304, 476, 1464, 1669, 1771, 1867, 2842
BseRI	GAGGAG	1 cut(s)	2366
BseSI	GKGCMC	1 cut(s)	1522
BseX3I	CGGCCG	1 cut(s)	330
BseYI	CCCAGC	1 cut(s)	2530
BsgI	GTGCAG	2 cut(s)	2838, 2981
Bsh1236I	CGCG	1 cut(s)	2322
Bsh1285I	CGRYCG	1 cut(s)	333
BshFI	GGCC	8 cut(s)	178, 228, 241, 332, 597, 991, 1116, 2735
BshNI	GGYRCC	1 cut(s)	1541
BsiEI	CGRYCG	1 cut(s)	333
BsiHKAI	GWGCWC	2 cut(s)	525, 1980
BsiHKCI	CYCGRG	2 cut(s)	363, 1979
BsiSI	CCGG	5 cut(s)	149, 193, 879, 1013, 2054
BslFI	GGGAC	2 cut(s)	1722, 1804
BslI	CCNNNNNNNGG	2 cut(s)	998, 2897
BsmAI	GTCTC	1 cut(s)	161
BsmFI	GGGAC	2 cut(s)	1722, 1804
BsmI	GAATGC	1 cut(s)	2425
BsnI	GGCC	8 cut(s)	178, 228, 241, 332, 597, 991, 1116, 2735
BsoBI	CYCGRG	2 cut(s)	363, 1979
Bsp1286I	GDGCHC	3 cut(s)	525, 1522, 1980
Bsp13I	TCCGGA	1 cut(s)	1012
Bsp1407I	TGTACA	1 cut(s)	1810
Bsp1720I	GCTNAGC	3 cut(s)	350, 864, 1740
Bsp19I	CCATGG	1 cut(s)	1488
BspANI	GGCC	8 cut(s)	178, 228, 241, 332, 597, 991, 1116, 2735
BspCNI	CTCAG	7 cut(s)	184, 337, 1365, 1444, 1732, 2608, 2647
BspEI	TCCGGA	1 cut(s)	1012
BspFNI	CGCG	1 cut(s)	2322
BspLI	GGNNCC	5 cut(s)	154, 1543, 1628, 2302, 2889
BspQI	GCTCTTC	1 cut(s)	2346
BspT107I	GGYRCC	1 cut(s)	1541
BsrBI	CCGCTC	2 cut(s)	1151, 2465
BsrDI	GCAATG	3 cut(s)	1574, 1813, 2273
BsrFI	RCCGGY	1 cut(s)	2053
BsrGI	TGTACA	1 cut(s)	1810
BsrI	ACTGG	7 cut(s)	304, 476, 1464, 1669, 1771, 1867, 2842
BssAI	RCCGGY	1 cut(s)	2053
BssECI	CCNNGG	4 cut(s)	531, 1314, 1488, 2130
BssNI	GRCGYC	3 cut(s)	125, 2426, 2859
BssSI	CACGAG	1 cut(s)	1637
BssT1I	CCWWGG	4 cut(s)	531, 1314, 1488, 2130
Bst2BI	CACGAG	1 cut(s)	1637
Bst2UI	CCWGG	2 cut(s)	1104, 1631
Bst4CI	ACNGT	1 cut(s)	752
Bst6I	CTCTTC	8 cut(s)	30, 73, 121, 282, 287, 1284, 1488, 2346
BstACI	GRCGYC	3 cut(s)	125, 2426, 2859
BstAPI	GCANNNNNTGC	1 cut(s)	2420
BstAUI	TGTACA	1 cut(s)	1810
BstBAI	YACGTR	1 cut(s)	438
BstC8I	GCNNGC	9 cut(s)	180, 356, 595, 845, 861, 1300, 1533, 2051, 2964
BstDSI	CCRYGG	1 cut(s)	1488
BstEII	GGTNACC	1 cut(s)	746
BstENI	CCTNNNNNAGG	1 cut(s)	2895
BstFNI	CGCG	1 cut(s)	2322
BstHHI	GCGC	1 cut(s)	2160
BstMAI	GTCTC	1 cut(s)	161
BstMCI	CGRYCG	1 cut(s)	333
BstNI	CCWGG	2 cut(s)	1104, 1631
BstNSI	RCATGY	3 cut(s)	372, 792, 872
BstPAI	GACNNNNGTC	1 cut(s)	702
BstPI	GGTNACC	1 cut(s)	746
BstSCI	CCNGG	5 cut(s)	148, 192, 878, 1102, 1629
BstSLI	GKGCMC	1 cut(s)	1522
BstSNI	TACGTA	1 cut(s)	438
BstUI	CGCG	1 cut(s)	2322
BstV2I	GAAGAC	2 cut(s)	120, 2684
BstX2I	RGATCY	2 cut(s)	1170, 2239
BstXI	CCANNNNNNTGG	1 cut(s)	1552
BstYI	RGATCY	2 cut(s)	1170, 2239
BstZI	CGGCCG	1 cut(s)	330
BsuI	GTATCC	2 cut(s)	190, 1396
BsuRI	GGCC	8 cut(s)	178, 228, 241, 332, 597, 991, 1116, 2735
BtgI	CCRYGG	1 cut(s)	1488
BtgZI	GCGATG	2 cut(s)	1248, 1909
BtrI	CACGTC	1 cut(s)	653
BtsI	GCAGTG	1 cut(s)	2184
BtsIMutI	CAGTG	5 cut(s)	179, 1370, 1764, 2184, 2862
Cac8I	GCNNGC	9 cut(s)	180, 356, 595, 845, 861, 1300, 1533, 2051, 2964
CaiI	CAGNNNCTG	1 cut(s)	2723
CfoI	GCGC	1 cut(s)	2160
Cfr10I	RCCGGY	1 cut(s)	2053
Cfr13I	GGNCC	7 cut(s)	474, 704, 713, 1010, 1114, 1633, 2888
CseI	GACGC	1 cut(s)	2415
Csp6I	GTAC	4 cut(s)	327, 439, 720, 1811
CviQI	GTAC	4 cut(s)	327, 439, 720, 1811
DraI	TTTAAA	1 cut(s)	1615
DraIII	CACNNNGTG	1 cut(s)	1376
EaeI	YGGCCR	3 cut(s)	176, 239, 330
EagI	CGGCCG	1 cut(s)	330
Eam1104I	CTCTTC	8 cut(s)	30, 73, 121, 282, 287, 1284, 1488, 2346
EarI	CTCTTC	8 cut(s)	30, 73, 121, 282, 287, 1284, 1488, 2346
EciI	GGCGGA	1 cut(s)	68
Ecl136II	GAGCTC	1 cut(s)	523
EclXI	CGGCCG	1 cut(s)	330
Eco105I	TACGTA	1 cut(s)	438
Eco130I	CCWWGG	4 cut(s)	531, 1314, 1488, 2130
Eco24I	GRGCYC	1 cut(s)	525
Eco47I	GGWCC	6 cut(s)	474, 704, 713, 1010, 1633, 2888
Eco52I	CGGCCG	1 cut(s)	330
Eco53kI	GAGCTC	1 cut(s)	523
Eco57I	CTGAAG	7 cut(s)	199, 1203, 1464, 1988, 2085, 2169, 2558
Eco88I	CYCGRG	2 cut(s)	363, 1979
Eco91I	GGTNACC	1 cut(s)	746
EcoICRI	GAGCTC	1 cut(s)	523
EcoNI	CCTNNNNNAGG	1 cut(s)	2895
EcoO109I	RGGNCCY	1 cut(s)	2888
EcoO65I	GGTNACC	1 cut(s)	746
EcoRII	CCWGG	2 cut(s)	1102, 1629
EcoT14I	CCWWGG	4 cut(s)	531, 1314, 1488, 2130
EcoT22I	ATGCAT	1 cut(s)	2416
EcoT38I	GRGCYC	1 cut(s)	525
ErhI	CCWWGG	4 cut(s)	531, 1314, 1488, 2130
FalI	AAGNNNNNCTT	6 cut(s)	582, 614, 2127, 2159, 2910, 2942
FaqI	GGGAC	2 cut(s)	1722, 1804
FauI	CCCGC	2 cut(s)	2327, 2900
FauNDI	CATATG	2 cut(s)	1408, 1950
FbaI	TGATCA	2 cut(s)	1057, 1993
FblI	GTMKAC	3 cut(s)	122, 1724, 2193
FriOI	GRGCYC	1 cut(s)	525
FspBI	CTAG	6 cut(s)	54, 618, 987, 1679, 1695, 1712
GlaI	GCGC	1 cut(s)	2159
GsaI	CCCAGC	1 cut(s)	2534
GsuI	CTGGAG	2 cut(s)	1884, 2443
HaeIII	GGCC	8 cut(s)	178, 228, 241, 332, 597, 991, 1116, 2735
HapII	CCGG	5 cut(s)	149, 193, 879, 1013, 2054
HgaI	GACGC	1 cut(s)	2415
HhaI	GCGC	1 cut(s)	2160
Hin1I	GRCGYC	3 cut(s)	125, 2426, 2859
Hin6I	GCGC	1 cut(s)	2158
HinP1I	GCGC	1 cut(s)	2158
HincII	GTYRAC	7 cut(s)	123, 772, 900, 1024, 1033, 1903, 2874
HindII	GTYRAC	7 cut(s)	123, 772, 900, 1024, 1033, 1903, 2874
HindIII	AAGCTT	2 cut(s)	1300, 1729
HinfI	GANTC	7 cut(s)	169, 189, 558, 805, 1607, 2084, 2657
HpaI	GTTAAC	1 cut(s)	772
HpaII	CCGG	5 cut(s)	149, 193, 879, 1013, 2054
HphI	GGTGA	3 cut(s)	593, 1237, 1892
Hpy99I	CGWCG	4 cut(s)	124, 127, 140, 1149
HpyCH4III	ACNGT	1 cut(s)	752
HpyCH4IV	ACGT	5 cut(s)	125, 437, 652, 1704, 2859
HpySE526I	ACGT	5 cut(s)	125, 437, 652, 1704, 2859
Hsp92I	GRCGYC	3 cut(s)	125, 2426, 2859
HspAI	GCGC	1 cut(s)	2158
Kpn2I	TCCGGA	1 cut(s)	1012
Ksp22I	TGATCA	2 cut(s)	1057, 1993
KspAI	GTTAAC	1 cut(s)	772
LguI	GCTCTTC	1 cut(s)	2346
LmnI	GCTCC	3 cut(s)	520, 983, 1933
MaeI	CTAG	6 cut(s)	54, 618, 987, 1679, 1695, 1712
MaeII	ACGT	5 cut(s)	125, 437, 652, 1704, 2859
MaeIII	GTNAC	7 cut(s)	371, 467, 746, 1455, 2380, 2389, 2860
MbiI	CCGCTC	2 cut(s)	1151, 2465
MfeI	CAATTG	3 cut(s)	1083, 2007, 2220
MflI	RGATCY	2 cut(s)	1170, 2239
MhlI	GDGCHC	3 cut(s)	525, 1522, 1980
MlsI	TGGCCA	2 cut(s)	178, 241
MluNI	TGGCCA	2 cut(s)	178, 241
MlyI	GAGTC	3 cut(s)	163, 198, 2666
MmeI	TCCRAC	1 cut(s)	809
Mox20I	TGGCCA	2 cut(s)	178, 241
Mph1103I	ATGCAT	1 cut(s)	2416
MroI	TCCGGA	1 cut(s)	1012
MroXI	GAANNNNTTC	1 cut(s)	2681
MscI	TGGCCA	2 cut(s)	178, 241
MseI	TTAA	6 cut(s)	527, 771, 1284, 1427, 1614, 2847
MslI	CAYNNNNRTG	4 cut(s)	1223, 1550, 1641, 1949
Msp20I	TGGCCA	2 cut(s)	178, 241
MspA1I	CMGCKG	4 cut(s)	1691, 2654, 2698, 2723
MspI	CCGG	5 cut(s)	149, 193, 879, 1013, 2054
MspR9I	CCNGG	5 cut(s)	150, 194, 880, 1104, 1631
MunI	CAATTG	3 cut(s)	1083, 2007, 2220
Mva1269I	GAATGC	1 cut(s)	2425
MvaI	CCWGG	2 cut(s)	1104, 1631
MvnI	CGCG	1 cut(s)	2322
NciI	CCSGG	3 cut(s)	150, 194, 880
NcoI	CCATGG	1 cut(s)	1488
NdeI	CATATG	2 cut(s)	1408, 1950
NlaIV	GGNNCC	5 cut(s)	154, 1543, 1628, 2302, 2889
NmeAIII	GCCGAG	1 cut(s)	2534
NmuCI	GTSAC	2 cut(s)	2389, 2860
NsiI	ATGCAT	1 cut(s)	2416
NspI	RCATGY	3 cut(s)	372, 792, 872
OliI	CACNNNNGTG	1 cut(s)	1641
PciI	ACATGT	1 cut(s)	788
PciSI	GCTCTTC	1 cut(s)	2346
PctI	GAATGC	1 cut(s)	2425
PdmI	GAANNNNTTC	1 cut(s)	2681
PfeI	GAWTC	4 cut(s)	558, 805, 1607, 2084
PflMI	CCANNNNNTGG	1 cut(s)	998
PfoI	TCCNGGA	2 cut(s)	192, 1629
PleI	GAGTC	3 cut(s)	163, 197, 2665
PpsI	GAGTC	3 cut(s)	163, 197, 2665
Ppu21I	YACGTR	1 cut(s)	438
PpuMI	RGGWCCY	1 cut(s)	2888
PscI	ACATGT	1 cut(s)	788
PshAI	GACNNNNGTC	1 cut(s)	702
Psp124BI	GAGCTC	1 cut(s)	525
Psp5II	RGGWCCY	1 cut(s)	2888
Psp6I	CCWGG	2 cut(s)	1102, 1629
PspEI	GGTNACC	1 cut(s)	746
PspFI	CCCAGC	1 cut(s)	2530
PspGI	CCWGG	2 cut(s)	1102, 1629
PspN4I	GGNNCC	5 cut(s)	154, 1543, 1628, 2302, 2889
PspPI	GGNCC	7 cut(s)	474, 704, 713, 1010, 1114, 1633, 2888
PspPPI	RGGWCCY	1 cut(s)	2888
PstNI	CAGNNNCTG	1 cut(s)	2723
PsuI	RGATCY	2 cut(s)	1170, 2239
PvuII	CAGCTG	4 cut(s)	1691, 2654, 2698, 2723
RsaI	GTAC	4 cut(s)	328, 440, 721, 1812
RsaNI	GTAC	4 cut(s)	327, 439, 720, 1811
RseI	CAYNNNNRTG	4 cut(s)	1223, 1550, 1641, 1949
SacI	GAGCTC	1 cut(s)	525
SalI	GTCGAC	1 cut(s)	121
SapI	GCTCTTC	1 cut(s)	2346
SaqAI	TTAA	6 cut(s)	527, 771, 1284, 1427, 1614, 2847
Sau96I	GGNCC	7 cut(s)	474, 704, 713, 1010, 1114, 1633, 2888
SchI	GAGTC	3 cut(s)	163, 198, 2666
ScrFI	CCNGG	5 cut(s)	150, 194, 880, 1104, 1631
SduI	GDGCHC	3 cut(s)	525, 1522, 1980
SgrDI	CGTCGACG	1 cut(s)	121
SinI	GGWCC	6 cut(s)	474, 704, 713, 1010, 1633, 2888
SmiMI	CAYNNNNRTG	4 cut(s)	1223, 1550, 1641, 1949
SmlI	CTYRAG	1 cut(s)	2955
SmoI	CTYRAG	1 cut(s)	2955
SnaBI	TACGTA	1 cut(s)	438
SpeI	ACTAGT	1 cut(s)	1711
SspI	AATATT	2 cut(s)	318, 1818
SspMI	CTAG	6 cut(s)	54, 618, 987, 1679, 1695, 1712
SstI	GAGCTC	1 cut(s)	525
StyD4I	CCNGG	5 cut(s)	148, 192, 878, 1102, 1629
StyI	CCWWGG	4 cut(s)	531, 1314, 1488, 2130
TaaI	ACNGT	1 cut(s)	752
TaiI	ACGT	5 cut(s)	128, 440, 655, 1707, 2862
TaqI	TCGA	9 cut(s)	122, 207, 507, 556, 891, 1147, 1511, 2082, 2768
TatI	WGTACW	2 cut(s)	719, 1810
TauI	GCSGC	4 cut(s)	84, 1135, 2426, 2969
TfiI	GAWTC	4 cut(s)	558, 805, 1607, 2084
Tru1I	TTAA	6 cut(s)	527, 771, 1284, 1427, 1614, 2847
Tru9I	TTAA	6 cut(s)	527, 771, 1284, 1427, 1614, 2847
TscAI	CASTG	5 cut(s)	179, 1377, 1771, 2184, 2869
TseFI	GTSAC	2 cut(s)	2389, 2860
Tsp45I	GTSAC	2 cut(s)	2389, 2860
TspDTI	ATGAA	7 cut(s)	314, 937, 2127, 2280, 2595, 2805, 2973
TspGWI	ACGGA	5 cut(s)	39, 92, 124, 696, 2960
TspRI	CASTG	5 cut(s)	179, 1377, 1771, 2184, 2869
Van91I	CCANNNNNTGG	1 cut(s)	998
VpaK11BI	GGWCC	6 cut(s)	474, 704, 713, 1010, 1633, 2888
XagI	CCTNNNNNAGG	1 cut(s)	2895
XapI	RAATTY	1 cut(s)	1334
XceI	RCATGY	3 cut(s)	372, 792, 872
XmiI	GTMKAC	3 cut(s)	122, 1724, 2193
XmnI	GAANNNNTTC	1 cut(s)	2681
XspI	CTAG	6 cut(s)	54, 618, 987, 1679, 1695, 1712
ZraI	GACGTC	2 cut(s)	126, 2860
Zsp2I	ATGCAT	1 cut(s)	2416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.