Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010393

Physical Location & Seq

Genomic Coordinates Forward (+)

39943 .. 48426

Gene Structure 8484 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1000800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3051 bp

ATGAGGAGTGGAATTCGTGAGAAAGGAGCTATAGCCTTCCGCGCCCTTCTCATTTCACTGCCTTGCCCTCGTTTCACGTCTCCTTCTACTCACACTCATTTGCCGCCGCCACTCCTCTATCGCGGACGGCCCCTCGCCGCTGCCTACAGGTGCCACTCATCCTCGGCGAAGGCCTCGACTCCCTCCGCCATGCCGGGCGCTGAATCTCAGGGGCTGGAGTGGCCGGCGAAGCGCGTCAGGGACACCTTCGTCAAGTTTTTCGAGGAGAAGGGCCACGTCTTCTGGAAGTCTAGCCCCGTCGTTCCCCACAACGATCCCACTCTTTTATTCGCCAATGCTGGTATGAACCAGTTCAAGCCCATTTTTCTGGGGACAGCGGACCCAAACACTCAGTTGAGCAAGCTGACTCGAGCATGCAACACCCAGAAATGTATAAGAGCAGGCGGGAAGCACAACGATCTCGATGATGTAGGGAAAGACACTTACCACCACACCTTCTTTGAGATGCTTGGCAATTGGTCTTTTGGGGATTATTTCAAGAACGAAGCCATTGGATGGGCTTGGGAGCTTCTCACAAAAGTTTATAAGCTGCCAGAAGATCGAATTTATGCCACCTATTTTGGTGGTGATGAGAAGGCTGGTCTTGCTCCTGATGATGAAGCTAGAGAGTTGTGGCTTAAGTTTCTACCAGCTGGTCATGTACTACCGTTTGGCTGTAAGGATAACTTCTGGGAGATGGGAGATACTGGTCCTTGTGGTCCTTGCACTGAAATTCATTATGATAGAATCGGAAATCGTGATGCTGCATCGTTAGTCAACAACGATGATCCTACCTGCATTGAAATTTGGAACCTTGTCTTTATTCAGTTCAATAGGGAAAGTGATGGTTCTCTAAAACCGCTCCCTGCTAAGCATGTTGACACTGGGATGGGATTTGAAAGATTAACTTCCATACTTCAGAACAAGATGAGCAATTATGATACTGATGTGTTCATGCCCATCTTTGATGCTATCCAACAGGCAACAGGGGCTCCACCATATTCTGGGAAAGTTGGTGTGGATGATGTAGACAAAATCGACATGGCATACAGAGTTGTTGCAGATCATATAAGAACTCTTTCATTTTCCATTGCTGATGGGTCTCGTCCAGGCAATGACGGCCGCGAATATGTTCTGAGGCGCATTCTTCGTCGCGCTGTTCGGTATGGAAATGATGTCCTAAAGGCTAAAGAAGGATTTTTCAATGGGCTTGTGCACATTCTGGTGACACTGATGGGTGATGTTTTCCCAGAGGTACAACAACATGAAGCACATATCAGGGAGATAATCAAAGAGGAGGAAGAAACTTTTGAGAAGACTTTACAGAAGGGAATAGAAAGATTCAAGAAGGCTGCTCAGGATGTTCAAGGGAAAACATTTAGTGGGCAGGATGCTTTTGTTTTGTGGGACACATTTGGGTTTCCATTAGATTTGACTCAGTTGATGGCAGAGGAAAGGGGATTGATGGTTGATGTTGAGGGTTTCAATAATGCTATGGATGAGGCTCGAGAAAGATCAAGGAGCGCTCAAAATAAGCAAGCTGGTGGTGCTATTGTTATGGATGCTGATGCGACTGCTACATTGCACAAGAGGGGAGTTGCTGCAACAGATGACAGCTTTAAATTTATTTGGAACCAGGACCATCAAAGTGTGGTAAAAGCAATTTACACTGGTTCTGAGTTTATTAATAGTGCTTCTGCTGGCAATGAAGTTGGTATAGTTTTGGAGTCTACAAATTTTTATGCTGAGCAGGGTGGTCAGGATATTTTTGATACTGGATCACTTGAAGGATCTTCTTTCTCGTTTCAAGTTTGCAATGTTCAAATTTATGGAGGTTTTGTTGTCCACATTGGTTCTTTTTCTGGAGAGAGTGGCAAACTCTTAGTCGGTGAAAAAGTAAATTGCAAGGTTGATTACAATAGGCGTAAACTCATTGCCCCTAACCATACCTGCACGCACATGTTGAATTTTGCTCTGAGGGAAGTTCTTGGAAATCATGTCGACCAGAAGGGTTCTATTGTTCTTGCTGAGAAATTGAGATTTGATTTTTCACATGGCAAGCCGGTAGATCCTGACAGTTTGAGAAGAATAGAGTCAATTGTGAATGAGCAAATCAGAGCTGAATTAGGTGTATATGCTAAAGAGGTAGCCCTGGTTGATGCCAAGCGCATCAATGGTTTAAGGGCTGTATTTGGAGAGGTATATCCTGACCCAGTAAGAGTGGTGGCTATTGGGCGAAAGGTTGAGGATCTTTTGGATTACCCCGAAAATGAGGAATGGTTATCAATTTCAGCAGAACTTTGTGGAGGGACACATATATCAAATACACGGGAAGCAAAGGCCTTTGCTCTTTTATCTGAGGAGGGAATTGCTAAGGGAATACGGAGAATAACTGCTGTCACAAGTGAAGGTGCTTTTCAGGCAATTGAATTGGCTCGGTCGCTTGAGCAAGACGTACATGAAGCGTCCAAGGCTGAAGGAAGCTTGCTGGAGAAGAAAGTAGCTTCCCTAAAATCTCGTGTGGACTCAGCACCAATTCCAGCTGCTAAGAAAGCTGAAATCAGGACCAAGATGGCTGTACTTCAGACCCAATTGAAGAAAGCACAGAAGATGATTGCAGAGCAAAATATAAAGAATGCTGTCAAGGTTGCAACTGAGATGGCTGAAGTTGCTGCTTCAGATGGGAAAGCTTACTGTGTTTCCCAAGTTGATGTTGGTTTGGATACAGCTGCAGTTCGTGAGGCTGTTTTGAAGATCACTGGGATGGGAATGCCTGCCATGGTTTTCAGCACGGATGAGACCACAAATAAAGCTTTGGTGTGTGCTGGCGTACCAGGGGACAAGGGCACTCAGTTGGAGGTCTCAGAGTGGTTGACAGCAGCTATGGGTCCTTTGAATGGAAGATGCGGAAAAGGAAAAGGTGGCCTTGCTACTGGCCAGGGAACAGATGCATCTCGTCTGAATGAGGCGATAGACCTTGCAACGAGTTTTGCACAGATGAAATTGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1017

Amino Acids

111.65

Weight (kDa)

6.01

Isoelectric Point (pI)

35.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	79 - 653	1.6e-219	tRNA synthetases class II (A)
tRNA_SAD	PF07973	752 - 810	4.4e-14	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	909 - 1011	1.6e-08	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50200 AT1G50200
fragaria_vesca	FvH4_4g36780 FvH4_4g36780
malus_domestica	MD13G1007000.v1.1 MD16G1000800.v1.1
prunus_persica	Prupe.1G345900_v2.0.a1
pyrus_communis	pycom13g00590 pycom16g00460
rosa_chinensis	RchiOBHm_Chr4g0446511
rosa_laevigata	RLG00000005653
rosa_multiflora	Rmu_co8401425.1_g000001 Rmu_sc0005526.1_g000002 Rmu_sc0009884.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386930
rosa_rugosa	Rorug04G0372100 Rorug04G0372200
rosa_samantha	Rh4BG442800 Rh4CG460300 Rh4DG441500
rosa_wichuraiana	Rw4G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	585
AasI	GACNNNNNNGTC	1 cut(s)	248
Acc36I	ACCTGC	2 cut(s)	842, 1997
AccB1I	GGYRCC	1 cut(s)	150
AccB7I	CCANNNNNTGG	2 cut(s)	555, 1043
AccBSI	CCGCTC	1 cut(s)	901
AccI	GTMKAC	3 cut(s)	1068, 1769, 2040
AccII	CGCG	5 cut(s)	42, 123, 234, 1164, 1194
AclWI	GGATC	6 cut(s)	308, 821, 1825, 1837, 2102, 2295
AcoI	YGGCCR	3 cut(s)	221, 1159, 2974
AcsI	RAATTY	8 cut(s)	12, 603, 771, 843, 1661, 1774, 1863, 2005
AcuI	CTGAAG	5 cut(s)	941, 2535, 2606, 2700, 2724
AfaI	GTAC	5 cut(s)	702, 1296, 2496, 2619, 2871
AfeI	AGCGCT	1 cut(s)	1564
AfiI	CCNNNNNNNGG	3 cut(s)	555, 1043, 2936
AflII	CTTAAG	1 cut(s)	677
AflIII	ACRYGT	1 cut(s)	1998
AjiI	CACGTC	2 cut(s)	78, 277
AjnI	CCWGG	5 cut(s)	1147, 1674, 2190, 2872, 2976
Alw21I	GWGCWC	1 cut(s)	1257
Alw26I	GTCTC	4 cut(s)	84, 1146, 2831, 2905
Alw44I	GTGCAC	1 cut(s)	1253
AlwI	GGATC	6 cut(s)	308, 821, 1825, 1837, 2102, 2295
AlwNI	CAGNNNCTG	1 cut(s)	214
Ama87I	CYCGRG	2 cut(s)	408, 1545
Aor51HI	AGCGCT	1 cut(s)	1564
AoxI	GGCC	8 cut(s)	128, 171, 221, 271, 1159, 2379, 2962, 2974
ApaLI	GTGCAC	1 cut(s)	1253
ApeKI	GCWGC	9 cut(s)	140, 589, 803, 1391, 1640, 2582, 2711, 2768, 2918
ApoI	RAATTY	8 cut(s)	12, 603, 771, 843, 1661, 1774, 1863, 2005
ArsI	GACNNNNNNTTYG	2 cut(s)	1908, 1940
AseI	ATTAAT	1 cut(s)	1725
Asp700I	GAANNNNTTC	3 cut(s)	350, 1117, 1170
AspLEI	GCGC	7 cut(s)	44, 200, 234, 1182, 1196, 1565, 2208
AspS9I	GGNCC	8 cut(s)	129, 271, 379, 749, 758, 1678, 2604, 2927
AsuC2I	CCSGG	1 cut(s)	195
AsuHPI	GGTGA	4 cut(s)	638, 1276, 1289, 1940
AvaI	CYCGRG	2 cut(s)	408, 1545
AvaII	GGWCC	6 cut(s)	379, 749, 758, 1678, 2604, 2927
BaeGI	GKGCMC	2 cut(s)	1257, 2888
BaeI	ACNNNNGTAYC	2 cut(s)	972, 1005
BalI	TGGCCA	1 cut(s)	2976
BanI	GGYRCC	1 cut(s)	150
BanII	GRGCYC	1 cut(s)	1033
BauI	CACGAG	1 cut(s)	2556
BbsI	GAAGAC	2 cut(s)	271, 1361
Bbv12I	GWGCWC	1 cut(s)	1257
BbvI	GCAGC	9 cut(s)	127, 576, 790, 1378, 1627, 2569, 2698, 2755, 2930
BceAI	ACGGC	2 cut(s)	143, 1174
BciT130I	CCWGG	5 cut(s)	1149, 1676, 2192, 2874, 2978
BciVI	GTATCC	1 cut(s)	2755
BcnI	CCSGG	1 cut(s)	195
BcoDI	GTCTC	4 cut(s)	84, 1146, 2831, 2905
BfaI	CTAG	2 cut(s)	291, 663
BfmI	CTRYAG	3 cut(s)	30, 145, 2769
BfoI	RGCGCY	2 cut(s)	201, 1566
BfrI	CTTAAG	1 cut(s)	677
BfuAI	ACCTGC	2 cut(s)	842, 1997
BfuI	GTATCC	1 cut(s)	2755
BlpI	GCTNAGC	2 cut(s)	909, 1785
Bme1390I	CCNGG	6 cut(s)	195, 1149, 1676, 2192, 2874, 2978
Bme18I	GGWCC	6 cut(s)	379, 749, 758, 1678, 2604, 2927
BmeT110I	CYCGRG	2 cut(s)	408, 1545
BmgBI	CACGTC	2 cut(s)	78, 277
BmgT120I	GGNCC	8 cut(s)	129, 271, 379, 749, 758, 1678, 2604, 2927
BmiI	GGNNCC	7 cut(s)	131, 152, 381, 851, 1032, 1673, 2928
BmrFI	CCNGG	6 cut(s)	195, 1149, 1676, 2192, 2874, 2978
BmrI	ACTGGG	3 cut(s)	933, 2246, 2808
BmuI	ACTGGG	3 cut(s)	933, 2246, 2808
BpiI	GAAGAC	2 cut(s)	271, 1361
BpmI	CTGGAG	3 cut(s)	236, 1923, 2549
Bpu10I	CCTNAGC	2 cut(s)	1395, 2412
Bpu1102I	GCTNAGC	2 cut(s)	909, 1785
BpuEI	CTTGAG	1 cut(s)	2504
BpuMI	CCSGG	1 cut(s)	195
BsaI	GGTCTC	3 cut(s)	1146, 2831, 2905
BsaJI	CCNNGG	6 cut(s)	162, 2190, 2508, 2817, 2873, 2977
BsaXI	ACNNNNNCTCC	4 cut(s)	1015, 1045, 1863, 1893
Bsc4I	CCNNNNNNNGG	3 cut(s)	555, 1043, 2936
Bse118I	RCCGGY	2 cut(s)	223, 2101
Bse1I	ACTGG	8 cut(s)	349, 751, 928, 1714, 1819, 2252, 2803, 2977
Bse3DI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1861, 1971
BseBI	CCWGG	5 cut(s)	1149, 1676, 2192, 2874, 2978
BseDI	CCNNGG	6 cut(s)	162, 2190, 2508, 2817, 2873, 2977
BseLI	CCNNNNNNNGG	3 cut(s)	555, 1043, 2936
BseMI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1861, 1971
BseNI	ACTGG	8 cut(s)	349, 751, 928, 1714, 1819, 2252, 2803, 2977
BseRI	GAGGAG	5 cut(s)	19, 104, 278, 1349, 2414
BseSI	GKGCMC	2 cut(s)	1257, 2888
BseX3I	CGGCCG	1 cut(s)	1159
BseXI	GCAGC	9 cut(s)	127, 576, 790, 1378, 1627, 2569, 2698, 2755, 2930
BsgI	GTGCAG	1 cut(s)	1975
Bsh1236I	CGCG	5 cut(s)	42, 123, 234, 1164, 1194
Bsh1285I	CGRYCG	2 cut(s)	1162, 2480
BshFI	GGCC	8 cut(s)	130, 173, 223, 273, 1161, 2381, 2964, 2976
BshNI	GGYRCC	1 cut(s)	150
BsiEI	CGRYCG	2 cut(s)	1162, 2480
BsiHKAI	GWGCWC	1 cut(s)	1257
BsiHKCI	CYCGRG	2 cut(s)	408, 1545
BsiSI	CCGG	3 cut(s)	194, 224, 2102
BslFI	GGGAC	5 cut(s)	254, 385, 1460, 2362, 2891
BslI	CCNNNNNNNGG	3 cut(s)	555, 1043, 2936
BsmAI	GTCTC	4 cut(s)	84, 1146, 2831, 2905
BsmBI	CGTCTC	1 cut(s)	84
BsmFI	GGGAC	5 cut(s)	254, 385, 1460, 2362, 2891
BsmI	GAATGC	3 cut(s)	1182, 2680, 2814
BsnI	GGCC	8 cut(s)	130, 173, 223, 273, 1161, 2381, 2964, 2976
Bso31I	GGTCTC	3 cut(s)	1146, 2831, 2905
BsoBI	CYCGRG	2 cut(s)	408, 1545
Bsp1286I	GDGCHC	3 cut(s)	1033, 1257, 2888
Bsp1720I	GCTNAGC	2 cut(s)	909, 1785
Bsp19I	CCATGG	1 cut(s)	2817
BspANI	GGCC	8 cut(s)	130, 173, 223, 273, 1161, 2381, 2964, 2976
BspFNI	CGCG	5 cut(s)	42, 123, 234, 1164, 1194
BspLI	GGNNCC	7 cut(s)	131, 152, 381, 851, 1032, 1673, 2928
BspMAI	CTGCAG	1 cut(s)	2773
BspMI	ACCTGC	2 cut(s)	842, 1997
BspPI	GGATC	6 cut(s)	308, 821, 1825, 1837, 2102, 2295
BspT107I	GGYRCC	1 cut(s)	150
BspTI	CTTAAG	1 cut(s)	677
BspTNI	GGTCTC	3 cut(s)	1146, 2831, 2905
BsrBI	CCGCTC	1 cut(s)	901
BsrDI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1861, 1971
BsrFI	RCCGGY	2 cut(s)	223, 2101
BsrI	ACTGG	8 cut(s)	349, 751, 928, 1714, 1819, 2252, 2803, 2977
BssAI	RCCGGY	2 cut(s)	223, 2101
BssECI	CCNNGG	6 cut(s)	162, 2190, 2508, 2817, 2873, 2977
BssSI	CACGAG	1 cut(s)	2556
BssT1I	CCWWGG	2 cut(s)	2508, 2817
Bst2BI	CACGAG	1 cut(s)	2556
Bst2UI	CCWGG	5 cut(s)	1149, 1676, 2192, 2874, 2978
Bst4CI	ACNGT	3 cut(s)	708, 2117, 2735
BstAFI	CTTAAG	1 cut(s)	677
BstDSI	CCRYGG	1 cut(s)	2817
BstFNI	CGCG	5 cut(s)	42, 123, 234, 1164, 1194
BstH2I	RGCGCY	2 cut(s)	201, 1566
BstHHI	GCGC	7 cut(s)	44, 200, 234, 1182, 1196, 1565, 2208
BstMAI	GTCTC	4 cut(s)	84, 1146, 2831, 2905
BstMCI	CGRYCG	2 cut(s)	1162, 2480
BstNI	CCWGG	5 cut(s)	1149, 1676, 2192, 2874, 2978
BstNSI	RCATGY	3 cut(s)	417, 917, 2002
BstSCI	CCNGG	6 cut(s)	193, 1147, 1674, 2190, 2872, 2976
BstSFI	CTRYAG	3 cut(s)	30, 145, 2769
BstSLI	GKGCMC	2 cut(s)	1257, 2888
BstUI	CGCG	5 cut(s)	42, 123, 234, 1164, 1194
BstV1I	GCAGC	9 cut(s)	127, 576, 790, 1378, 1627, 2569, 2698, 2755, 2930
BstV2I	GAAGAC	2 cut(s)	271, 1361
BstX2I	RGATCY	3 cut(s)	1829, 2107, 2287
BstXI	CCANNNNNNTGG	1 cut(s)	367
BstYI	RGATCY	3 cut(s)	1829, 2107, 2287
BstZI	CGGCCG	1 cut(s)	1159
BsuI	GTATCC	1 cut(s)	2755
BsuRI	GGCC	8 cut(s)	130, 173, 223, 273, 1161, 2381, 2964, 2976
BtgI	CCRYGG	1 cut(s)	2817
BtrI	CACGTC	2 cut(s)	78, 277
BtsI	GCAGTG	1 cut(s)	56
BtsIMutI	CAGTG	6 cut(s)	56, 765, 921, 1268, 1707, 2796
BveI	ACCTGC	2 cut(s)	842, 1997
CaiI	CAGNNNCTG	1 cut(s)	214
CfoI	GCGC	7 cut(s)	44, 200, 234, 1182, 1196, 1565, 2208
Cfr10I	RCCGGY	2 cut(s)	223, 2101
Cfr13I	GGNCC	8 cut(s)	129, 271, 379, 749, 758, 1678, 2604, 2927
CseI	GACGC	2 cut(s)	223, 2493
Csp6I	GTAC	5 cut(s)	701, 1295, 2495, 2618, 2870
CspCI	CAANNNNNGTGG	2 cut(s)	601, 636
CviQI	GTAC	5 cut(s)	701, 1295, 2495, 2618, 2870
DraI	TTTAAA	1 cut(s)	1660
DrdI	GACNNNNNNGTC	1 cut(s)	248
DseDI	GACNNNNNNGTC	1 cut(s)	248
EaeI	YGGCCR	3 cut(s)	221, 1159, 2974
EagI	CGGCCG	1 cut(s)	1159
EciI	GGCGGA	1 cut(s)	175
EclXI	CGGCCG	1 cut(s)	1159
Eco130I	CCWWGG	2 cut(s)	2508, 2817
Eco147I	AGGCCT	2 cut(s)	173, 2381
Eco24I	GRGCYC	1 cut(s)	1033
Eco31I	GGTCTC	3 cut(s)	1146, 2831, 2905
Eco47I	GGWCC	6 cut(s)	379, 749, 758, 1678, 2604, 2927
Eco47III	AGCGCT	1 cut(s)	1564
Eco52I	CGGCCG	1 cut(s)	1159
Eco57I	CTGAAG	5 cut(s)	941, 2535, 2606, 2700, 2724
Eco88I	CYCGRG	2 cut(s)	408, 1545
EcoO109I	RGGNCCY	1 cut(s)	2927
EcoRI	GAATTC	1 cut(s)	12
EcoRII	CCWGG	5 cut(s)	1147, 1674, 2190, 2872, 2976
EcoT14I	CCWWGG	2 cut(s)	2508, 2817
EcoT22I	ATGCAT	1 cut(s)	2992
EcoT38I	GRGCYC	1 cut(s)	1033
ErhI	CCWWGG	2 cut(s)	2508, 2817
Esp3I	CGTCTC	1 cut(s)	84
FalI	AAGNNNNNCTT	6 cut(s)	710, 742, 931, 963, 2949, 2981
FaqI	GGGAC	5 cut(s)	254, 385, 1460, 2362, 2891
FauI	CCCGC	1 cut(s)	437
FblI	GTMKAC	3 cut(s)	1068, 1769, 2040
FriOI	GRGCYC	1 cut(s)	1033
FspBI	CTAG	2 cut(s)	291, 663
GlaI	GCGC	7 cut(s)	43, 199, 233, 1181, 1195, 1564, 2207
GsuI	CTGGAG	3 cut(s)	236, 1923, 2549
HaeII	RGCGCY	2 cut(s)	201, 1566
HaeIII	GGCC	8 cut(s)	130, 173, 223, 273, 1161, 2381, 2964, 2976
HapII	CCGG	3 cut(s)	194, 224, 2102
HgaI	GACGC	2 cut(s)	223, 2493
HhaI	GCGC	7 cut(s)	44, 200, 234, 1182, 1196, 1565, 2208
Hin6I	GCGC	7 cut(s)	42, 198, 232, 1180, 1194, 1563, 2206
HinP1I	GCGC	7 cut(s)	42, 198, 232, 1180, 1194, 1563, 2206
HincII	GTYRAC	4 cut(s)	817, 919, 2041, 2913
HindII	GTYRAC	4 cut(s)	817, 919, 2041, 2913
HindIII	AAGCTT	3 cut(s)	2521, 2727, 2850
HinfI	GANTC	9 cut(s)	178, 203, 406, 786, 1380, 1474, 1766, 2132, 2564
HpaII	CCGG	3 cut(s)	194, 224, 2102
HphI	GGTGA	4 cut(s)	638, 1276, 1289, 1940
Hpy99I	CGWCG	2 cut(s)	302, 1194
HpyCH4III	ACNGT	3 cut(s)	708, 2117, 2735
HpyCH4IV	ACGT	3 cut(s)	77, 276, 2493
HpySE526I	ACGT	3 cut(s)	77, 276, 2493
HspAI	GCGC	7 cut(s)	42, 198, 232, 1180, 1194, 1563, 2206
KroI	GCCGGC	1 cut(s)	223
KroNI	GCCGGC	1 cut(s)	225
LmnI	GCTCC	6 cut(s)	26, 565, 652, 906, 1036, 1560
Lsp1109I	GCAGC	9 cut(s)	127, 576, 790, 1378, 1627, 2569, 2698, 2755, 2930
MaeI	CTAG	2 cut(s)	291, 663
MaeII	ACGT	3 cut(s)	77, 276, 2493
MaeIII	GTNAC	2 cut(s)	1264, 2437
MbiI	CCGCTC	1 cut(s)	901
MfeI	CAATTG	4 cut(s)	514, 2136, 2463, 2630
MflI	RGATCY	3 cut(s)	1829, 2107, 2287
MhlI	GDGCHC	3 cut(s)	1033, 1257, 2888
MlsI	TGGCCA	1 cut(s)	2976
MluNI	TGGCCA	1 cut(s)	2976
MlyI	GAGTC	6 cut(s)	172, 400, 1468, 1775, 2141, 2558
MmeI	TCCRAC	2 cut(s)	1039, 2874
Mox20I	TGGCCA	1 cut(s)	2976
Mph1103I	ATGCAT	1 cut(s)	2992
MroNI	GCCGGC	1 cut(s)	223
MroXI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
MscI	TGGCCA	1 cut(s)	2976
MseI	TTAA	5 cut(s)	678, 944, 1659, 1725, 2219
MslI	CAYNNNNRTG	5 cut(s)	926, 1262, 1686, 1997, 2801
Msp20I	TGGCCA	1 cut(s)	2976
MspA1I	CMGCKG	5 cut(s)	140, 377, 692, 2582, 2768
MspCI	CTTAAG	1 cut(s)	677
MspI	CCGG	3 cut(s)	194, 224, 2102
MspR9I	CCNGG	6 cut(s)	195, 1149, 1676, 2192, 2874, 2978
MunI	CAATTG	4 cut(s)	514, 2136, 2463, 2630
Mva1269I	GAATGC	3 cut(s)	1182, 2680, 2814
MvaI	CCWGG	5 cut(s)	1149, 1676, 2192, 2874, 2978
MvnI	CGCG	5 cut(s)	42, 123, 234, 1164, 1194
NaeI	GCCGGC	1 cut(s)	225
NciI	CCSGG	1 cut(s)	195
NcoI	CCATGG	1 cut(s)	2817
NgoMIV	GCCGGC	1 cut(s)	223
NlaIV	GGNNCC	7 cut(s)	131, 152, 381, 851, 1032, 1673, 2928
NmeAIII	GCCGAG	1 cut(s)	143
NmuCI	GTSAC	2 cut(s)	1264, 2437
NsiI	ATGCAT	1 cut(s)	2992
NspI	RCATGY	3 cut(s)	417, 917, 2002
PaeI	GCATGC	1 cut(s)	417
PaeR7I	CTCGAG	2 cut(s)	408, 1545
PceI	AGGCCT	2 cut(s)	173, 2381
PciI	ACATGT	1 cut(s)	1998
PctI	GAATGC	3 cut(s)	1182, 2680, 2814
PdiI	GCCGGC	1 cut(s)	225
PdmI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
PfeI	GAWTC	3 cut(s)	203, 786, 1380
PflMI	CCANNNNNTGG	2 cut(s)	555, 1043
PleI	GAGTC	6 cut(s)	172, 400, 1468, 1774, 2140, 2558
PpsI	GAGTC	6 cut(s)	172, 400, 1468, 1774, 2140, 2558
PpuMI	RGGWCCY	1 cut(s)	2927
PscI	ACATGT	1 cut(s)	1998
PshBI	ATTAAT	1 cut(s)	1725
PsiI	TTATAA	1 cut(s)	585
Psp5II	RGGWCCY	1 cut(s)	2927
Psp6I	CCWGG	5 cut(s)	1147, 1674, 2190, 2872, 2976
PspGI	CCWGG	5 cut(s)	1147, 1674, 2190, 2872, 2976
PspN4I	GGNNCC	7 cut(s)	131, 152, 381, 851, 1032, 1673, 2928
PspPI	GGNCC	8 cut(s)	129, 271, 379, 749, 758, 1678, 2604, 2927
PspPPI	RGGWCCY	1 cut(s)	2927
PspXI	VCTCGAGB	1 cut(s)	408
PstI	CTGCAG	1 cut(s)	2773
PstNI	CAGNNNCTG	1 cut(s)	214
PsuI	RGATCY	3 cut(s)	1829, 2107, 2287
PvuII	CAGCTG	3 cut(s)	692, 2582, 2768
RsaI	GTAC	5 cut(s)	702, 1296, 2496, 2619, 2871
RsaNI	GTAC	5 cut(s)	701, 1295, 2495, 2618, 2870
RseI	CAYNNNNRTG	5 cut(s)	926, 1262, 1686, 1997, 2801
SalI	GTCGAC	1 cut(s)	2039
SaqAI	TTAA	5 cut(s)	678, 944, 1659, 1725, 2219
Sau96I	GGNCC	8 cut(s)	129, 271, 379, 749, 758, 1678, 2604, 2927
SchI	GAGTC	6 cut(s)	172, 400, 1468, 1775, 2141, 2558
ScrFI	CCNGG	6 cut(s)	195, 1149, 1676, 2192, 2874, 2978
SduI	GDGCHC	3 cut(s)	1033, 1257, 2888
SfcI	CTRYAG	3 cut(s)	30, 145, 2769
Sfr274I	CTCGAG	2 cut(s)	408, 1545
SinI	GGWCC	6 cut(s)	379, 749, 758, 1678, 2604, 2927
SlaI	CTCGAG	2 cut(s)	408, 1545
SmiMI	CAYNNNNRTG	5 cut(s)	926, 1262, 1686, 1997, 2801
SmlI	CTYRAG	4 cut(s)	408, 677, 1545, 2483
SmoI	CTYRAG	4 cut(s)	408, 677, 1545, 2483
SphI	GCATGC	1 cut(s)	417
SseBI	AGGCCT	2 cut(s)	173, 2381
SspMI	CTAG	2 cut(s)	291, 663
StuI	AGGCCT	2 cut(s)	173, 2381
StyD4I	CCNGG	6 cut(s)	193, 1147, 1674, 2190, 2872, 2976
StyI	CCWWGG	2 cut(s)	2508, 2817
TaaI	ACNGT	3 cut(s)	708, 2117, 2735
TaiI	ACGT	3 cut(s)	80, 279, 2496
TaqI	TCGA	8 cut(s)	176, 261, 409, 462, 601, 1077, 1546, 2040
TaqII	GACCGA	1 cut(s)	2466
TatI	WGTACW	2 cut(s)	700, 2617
TauI	GCSGC	4 cut(s)	106, 109, 140, 1164
TfiI	GAWTC	3 cut(s)	203, 786, 1380
Tru1I	TTAA	5 cut(s)	678, 944, 1659, 1725, 2219
Tru9I	TTAA	5 cut(s)	678, 944, 1659, 1725, 2219
TscAI	CASTG	6 cut(s)	63, 772, 928, 1275, 1714, 2803
TseFI	GTSAC	2 cut(s)	1264, 2437
TseI	GCWGC	9 cut(s)	140, 589, 803, 1391, 1640, 2582, 2711, 2768, 2918
Tsp45I	GTSAC	2 cut(s)	1264, 2437
TspDTI	ATGAA	8 cut(s)	359, 672, 764, 982, 1110, 1320, 1761, 2514
TspGWI	ACGGA	2 cut(s)	2437, 2846
TspRI	CASTG	6 cut(s)	63, 772, 928, 1275, 1714, 2803
Van91I	CCANNNNNTGG	2 cut(s)	555, 1043
Vha464I	CTTAAG	1 cut(s)	677
VneI	GTGCAC	1 cut(s)	1253
VpaK11BI	GGWCC	6 cut(s)	379, 749, 758, 1678, 2604, 2927
VspI	ATTAAT	1 cut(s)	1725
XapI	RAATTY	8 cut(s)	12, 603, 771, 843, 1661, 1774, 1863, 2005
XceI	RCATGY	3 cut(s)	417, 917, 2002
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2750
XhoI	CTCGAG	2 cut(s)	408, 1545
XmiI	GTMKAC	3 cut(s)	1068, 1769, 2040
XmnI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
XspI	CTAG	2 cut(s)	291, 663
Zsp2I	ATGCAT	1 cut(s)	2992

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.