Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0010393

Physical Location & Seq

Genomic Coordinates Forward (+)

69110313 .. 69117836

Gene Structure 7524 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG460300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3042 bp

ATGAGTGGTGGATTCCTTGGGAAAGGAGTGATAGCGTTCAACTCTCTTCTTCTACTCAGACGTTACCCTTTCTCTTTCGCGCCGTCACACCTCCCTCGGAATCGGAATCGGAATCGCTCCCTCACCACCGCCAAGCACTTGGCCGCAATCGCAACGTCGTCGTTTCGTCCCAAGATGCCCGGCGTCGAATCTCAGGGAGGAGGACTAGAGTGGCCGGCACAGCGCGTGCGGGACACCTTCCTCGAGTTCTTCAGGGATGAGAAGAAACACGAGTACTGGAAGTCAAGCCCGGTCGTCCCCCACAACGATCCAACTCTTCTCTTTGCTAATGCTGGAATGAACCAGTTTAAGCCCATTTTTTTGGGGACGGCGGACCCGAACACTGGGCTAAGCAAGCTGACTAGAGCATGCAACACGCAGAAATGTATAAGGGCAGGAGGGAAGCATAATGATCTCGAAGATGTAGGGAAAGACACCTACCATCACACTTTTTTCGAGATGCTTGGTAATTGGTCGTTCGGGGACTATTTCAAGAGTGAAGCCATTGAGTGGGCTTGGAAGCTTCTCACTCAGGTTTATAAGCTGCCAGAAGATCGATTTTATGCCACTTATTTTGGCGGTGATGAGAAGCTGGGTCTTGCTCCTGATTATGAAGCAAGAGATTTATGGCTTAAGTTTCTACCACCTGGACGTGTACTGCCTTTTGGCTGTAAGGATAACTTTTGGGAGATGGGTGATACTGGTCCTTGCGGCCCTTGCACTGAGATCCATTATGATAGAGTTGGTAACCGGGATGCTGCATCATTAGTCAACTATGATGACCCTACCTGCCTCGAGATCTGGAACCTTGTCTTTATTCAGTTCAATCGGGAAAGTGATGGCTCTCTTAAACCATTGCCTGCTAAGCATGTTGACACTGGGATGGGATTTGAAAGATTGACTTCTGTACTTCAGAACAAGATGAGCAATTATGACACTGATGTCTTCATGCCTATATTTGATGCTATTCAGCAGGCTACAGGGGCTCGCCCATATTCTGGGAAAGTTGGAGATGATGATACAGACAAAGTTGACATGGCATACAGAGTTGTTGCAGATCATATAAGAACTCTTTCATTTGCCATTGCTGATGGGTCCCGTCCAGGCAATGATGGTCGTGAATATGTTCTGAGACGTATTCTCCGTCGAGCTGTCCGATATGGAAGAGAAGTGCTAAAAGCTCAAGAAGGATTTTTCAATGGGCTTGTACCGGTGCTGGCGGCACTGATGGGTGATGTATTTCCGGAGCTAAAACAACATGAAGCAGGCATTAGGGAGATAATCAAAGAGGAGGAAGAAGCATTTGGCAAGACTCTAATTAAGGGAATAGAAAAATTCAAAAAGGCAGCTCAAGATGTTCAAGGCAAAACATTTAGTGGGCAGGAGGCTTTTGTCTTGTGGGACACATTTGGGTTCCCGTTAGATTTGACTCAGTTGATGGCAGAGGAACAGGGATTAGAAGTTGATGTTGAGGGTTTCAATGTCGCTATGGATGAGGCTCGAGAAAGATCAAGGAATGCTCAAAATAAGGAAGAAGGTGGTGCCATTGTTATGAATGCTGAAGCTACTGCTTCATTGCACAAGAGTGGAATTGCTGCAACAGACGACAGCTTCAAATATATTTGGTTTCAGGACCATGAAAGTGTAATTAAAGCAATTTACACTGGTTCAGAGTTTATGGATAATGTTGCTGCTGGCAGCTTAGTTGGTGTTATTTTGGAGTCTACAAGTTTCTATGCAGAGCAAGGTGGACAGATATTTGATACTGGATCACTAAAAGGAGAGACCGGCTCATTTCAAGTCCGCAATGTTCAAATTTTTGGAGGTTTTGTTGTCCACATTGGTTCCTTCTCTGGAGAGCATGGCAAACTCTCTGTGGGTGACAAAGTGGTTTGTAAGGTTGACTATAAAAGGCGTACACTCATTGCGCCTAACCATACCTGCACACACATGTTGAACTTTGCTCTGAGGGAAGTTCTTGGCAGTCATGTTGACCAGAAGGGTTCCATTGTTCTTCCTGAAAAATTGAGATTTGATTTTTCTCATGGTAAGCCAGTGGACCCTGAACAGTTGAGAAGAATTGAATCAATTGTGAATGAGCAAATAAAAGCTGAATTAGATGTTTCTGTTAAGGAGGTAGCTCTGGTTGAAGCTGAGCGCATCAATGGTTTAAGGGCTGTATTTGGAGAGGTCTATCCTGACCCAGTAAGAGTTGTGGCTGTTGGGCGAAAAGTTGAGGACCTCCTGTCTGACCCTAAAAATGAGGAATGGTCATCAATATCAACAGAATTTTGTGGAGGGACCCATATAACAAACACACGTGAAGCTGAGGCCTTTGCCCTTTTATCTGAAGAGGGAACTGCTAAGGGAATCCGAAGGATAACTGCTGTCACCACTAATGCTGCTTTTGATGCAATTAAATTGGCAGATTCACTTGAGCAGGAAGTAATTGAAGCTTCCAAGGCTGAAGAGAGCTTGCTCGAGAAGAAGATAGCTTCCTTTAAATCTCGCCTAGACGCAGCACAAATTCCAGCGGCTAAGAAAGCTGATATCAGGGACAAGATTTCCCAACTTCAGAACAAAGTGAGAAAGGCACAGAAGAAGATTGCAGAGCAAAATTTACAGAAAGCTGTCAAGGTTGCAAGGGAGATGGCGGAAGTTGCTGCATCAGATGGCAAGTCTTTCTGTGTATCCCATGTTGATGTTGGTTTGGATACTGCTGCAGTTCGTGAAGCAGTTTGTAAAGTCATAGAGAAGGGAATGCCTGCGATGGTTTTTAGCACGGATGAAACAACAAATAAGGCTGTGGTGTGTGCTGGAGTACCAGGAGACAAAGGCAAGCAATTGGAGGTGTCAGAGTGGTTGACTGCAGCTATGGGTCCCCTGAAAGGAAGGGGTGGTAAAGGAAAAGGTGGCCTTGCTACTGGCCAGGGAACAGATGCATCGCATCTAAACGAGGCAATAGACCTGGCAACCACTTTTGCGCAGATGAAATTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1013

Amino Acids

111.79

Weight (kDa)

5.97

Isoelectric Point (pI)

34.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	76 - 651	5.2e-219	tRNA synthetases class II (A)
tRNA_SAD	PF07973	749 - 807	4.9e-12	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	906 - 1008	9.4e-10	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50200 AT1G50200
fragaria_vesca	FvH4_4g36780 FvH4_4g36780
malus_domestica	MD13G1007000.v1.1 MD16G1000800.v1.1
prunus_persica	Prupe.1G345900_v2.0.a1
pyrus_communis	pycom13g00590 pycom16g00460
rosa_chinensis	RchiOBHm_Chr4g0446511
rosa_laevigata	RLG00000005653
rosa_multiflora	Rmu_co8401425.1_g000001 Rmu_sc0005526.1_g000002 Rmu_sc0009884.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386930
rosa_rugosa	Rorug04G0372100 Rorug04G0372200
rosa_samantha	Rh4BG442800 Rh4CG460300 Rh4DG441500
rosa_wichuraiana	Rw4G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	579
AasI	GACNNNNNNGTC	1 cut(s)	980
Acc16I	TGCGCA	1 cut(s)	3024
Acc36I	ACCTGC	2 cut(s)	836, 1988
AccB1I	GGYRCC	1 cut(s)	1580
AccB7I	CCANNNNNTGG	2 cut(s)	549, 1037
AccI	GTMKAC	1 cut(s)	1763
AccII	CGCG	2 cut(s)	80, 225
AccIII	TCCGGA	1 cut(s)	1282
AclWI	GGATC	3 cut(s)	302, 760, 1816
AcoI	YGGCCR	3 cut(s)	141, 212, 2965
AcsI	RAATTY	5 cut(s)	1373, 1854, 2327, 2565, 2656
AcuI	CTGAAG	6 cut(s)	235, 935, 1620, 2409, 2526, 2597
AcvI	CACGTG	1 cut(s)	2360
AcyI	GRCGYC	1 cut(s)	183
AfaI	GTAC	6 cut(s)	275, 696, 948, 1248, 1957, 2862
AfiI	CCNNNNNNNGG	4 cut(s)	383, 549, 1037, 2927
AflII	CTTAAG	1 cut(s)	671
AflIII	ACRYGT	3 cut(s)	691, 1989, 2357
AgeI	ACCGGT	1 cut(s)	1249
AhdI	GACNNNNNGTC	1 cut(s)	2284
AjiI	CACGTC	1 cut(s)	692
AjnI	CCWGG	5 cut(s)	685, 1141, 2863, 2967, 3006
AjuI	GAANNNNNNNTTGG	2 cut(s)	1326, 1358
AleI	CACNNNNGTG	1 cut(s)	1623
Alw26I	GTCTC	3 cut(s)	1165, 1817, 2862
AlwI	GGATC	3 cut(s)	302, 760, 1816
Ama87I	CYCGRG	4 cut(s)	242, 833, 1539, 2519
Aor13HI	TCCGGA	1 cut(s)	1282
AoxI	GGCC	6 cut(s)	141, 212, 751, 2370, 2953, 2965
ApoI	RAATTY	5 cut(s)	1373, 1854, 2327, 2565, 2656
AsiGI	ACCGGT	1 cut(s)	1249
Asp700I	GAANNNNTTC	2 cut(s)	1111, 1164
AspLEI	GCGC	5 cut(s)	82, 225, 1969, 2199, 3025
AspS9I	GGNCC	9 cut(s)	373, 743, 752, 1134, 1672, 2098, 2278, 2340, 2918
AsuC2I	CCSGG	3 cut(s)	180, 290, 791
AsuHPI	GGTGA	6 cut(s)	115, 632, 746, 1283, 1931, 2422
AvaI	CYCGRG	4 cut(s)	242, 833, 1539, 2519
AvaII	GGWCC	8 cut(s)	373, 743, 1134, 1672, 2098, 2278, 2340, 2918
BalI	TGGCCA	1 cut(s)	2967
BanI	GGYRCC	1 cut(s)	1580
BanII	GRGCYC	1 cut(s)	1027
BauI	CACGAG	1 cut(s)	269
BbrPI	CACGTG	1 cut(s)	2360
BbsI	GAAGAC	1 cut(s)	976
BbvCI	CCTCAGC	1 cut(s)	2367
BceAI	ACGGC	2 cut(s)	67, 384
BcgI	CGANNNNNNTGC	2 cut(s)	141, 175
BciT130I	CCWGG	5 cut(s)	687, 1143, 2865, 2969, 3008
BciVI	GTATCC	2 cut(s)	2740, 2746
BcnI	CCSGG	3 cut(s)	180, 290, 791
BcoDI	GTCTC	3 cut(s)	1165, 1817, 2862
BfaI	CTAG	3 cut(s)	206, 402, 2552
BfmI	CTRYAG	3 cut(s)	1017, 2760, 2907
BfrI	CTTAAG	1 cut(s)	671
BfuAI	ACCTGC	2 cut(s)	836, 1988
BfuI	GTATCC	2 cut(s)	2740, 2746
BglII	AGATCT	1 cut(s)	837
BlpI	GCTNAGC	3 cut(s)	389, 903, 2193
BmcAI	AGTACT	1 cut(s)	275
Bme1390I	CCNGG	8 cut(s)	180, 290, 687, 791, 1143, 2865, 2969, 3008
Bme18I	GGWCC	8 cut(s)	373, 743, 1134, 1672, 2098, 2278, 2340, 2918
BmeRI	GACNNNNNGTC	1 cut(s)	2284
BmeT110I	CYCGRG	4 cut(s)	242, 833, 1539, 2519
BmgBI	CACGTC	1 cut(s)	692
BmgT120I	GGNCC	9 cut(s)	373, 743, 752, 1134, 1672, 2098, 2278, 2340, 2918
BmrFI	CCNGG	8 cut(s)	180, 290, 687, 791, 1143, 2865, 2969, 3008
BmrI	ACTGGG	3 cut(s)	393, 927, 2237
BmuI	ACTGGG	3 cut(s)	393, 927, 2237
BpiI	GAAGAC	1 cut(s)	976
BplI	GAGNNNNNCTC	2 cut(s)	1814, 1846
BpmI	CTGGAG	2 cut(s)	1914, 2877
Bpu10I	CCTNAGC	2 cut(s)	2367, 2403
Bpu1102I	GCTNAGC	3 cut(s)	389, 903, 2193
BpuEI	CTTGAG	3 cut(s)	1206, 1374, 2495
BpuMI	CCSGG	3 cut(s)	180, 290, 791
Bsa29I	ATCGAT	1 cut(s)	595
BsaAI	YACGTR	1 cut(s)	2360
BsaHI	GRCGYC	1 cut(s)	183
BsaI	GGTCTC	1 cut(s)	1817
BsaJI	CCNNGG	4 cut(s)	16, 95, 2499, 2968
BsaWI	WCCGGW	2 cut(s)	1249, 1282
BsaXI	ACNNNNNCTCC	4 cut(s)	1164, 1194, 1854, 1884
Bsc4I	CCNNNNNNNGG	4 cut(s)	383, 549, 1037, 2927
Bse118I	RCCGGY	3 cut(s)	214, 1249, 1826
Bse3DI	GCAATG	6 cut(s)	893, 1122, 1153, 1613, 1852, 1962
BseAI	TCCGGA	1 cut(s)	1282
BseBI	CCWGG	5 cut(s)	687, 1143, 2865, 2969, 3008
BseCI	ATCGAT	1 cut(s)	595
BseDI	CCNNGG	4 cut(s)	16, 95, 2499, 2968
BseGI	GGATG	5 cut(s)	262, 799, 927, 1537, 2830
BseLI	CCNNNNNNNGG	4 cut(s)	383, 549, 1037, 2927
BseMI	GCAATG	6 cut(s)	893, 1122, 1153, 1613, 1852, 1962
BseMII	CTCAG	9 cut(s)	70, 206, 584, 753, 1160, 1484, 1997, 2184, 2358
BseRI	GAGGAG	2 cut(s)	213, 1343
BseYI	CCCAGC	1 cut(s)	631
BsgI	GTGCAG	1 cut(s)	1966
Bsh1236I	CGCG	2 cut(s)	80, 225
Bsh1285I	CGRYCG	1 cut(s)	294
BshFI	GGCC	6 cut(s)	143, 214, 753, 2372, 2955, 2967
BshNI	GGYRCC	1 cut(s)	1580
BshTI	ACCGGT	1 cut(s)	1249
BshVI	ATCGAT	1 cut(s)	595
BsiEI	CGRYCG	1 cut(s)	294
BsiHKCI	CYCGRG	4 cut(s)	242, 833, 1539, 2519
BsiSI	CCGG	7 cut(s)	180, 215, 290, 790, 1250, 1283, 1827
BslI	CCNNNNNNNGG	4 cut(s)	383, 549, 1037, 2927
BsmAI	GTCTC	3 cut(s)	1165, 1817, 2862
BsmBI	CGTCTC	1 cut(s)	1165
BsmI	GAATGC	3 cut(s)	1561, 1600, 2805
BsnI	GGCC	6 cut(s)	143, 214, 753, 2372, 2955, 2967
Bso31I	GGTCTC	1 cut(s)	1817
BsoBI	CYCGRG	4 cut(s)	242, 833, 1539, 2519
Bsp1286I	GDGCHC	1 cut(s)	1027
Bsp13I	TCCGGA	1 cut(s)	1282
Bsp143I	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
Bsp1720I	GCTNAGC	3 cut(s)	389, 903, 2193
BspANI	GGCC	6 cut(s)	143, 214, 753, 2372, 2955, 2967
BspCNI	CTCAG	9 cut(s)	69, 205, 583, 754, 1161, 1483, 1998, 2185, 2359
BspDI	ATCGAT	1 cut(s)	595
BspEI	TCCGGA	1 cut(s)	1282
BspFNI	CGCG	2 cut(s)	80, 225
BspMAI	CTGCAG	2 cut(s)	2764, 2911
BspMI	ACCTGC	2 cut(s)	836, 1988
BspPI	GGATC	3 cut(s)	302, 760, 1816
BspT107I	GGYRCC	1 cut(s)	1580
BspTI	CTTAAG	1 cut(s)	671
BspTNI	GGTCTC	1 cut(s)	1817
BsrDI	GCAATG	6 cut(s)	893, 1122, 1153, 1613, 1852, 1962
BsrFI	RCCGGY	3 cut(s)	214, 1249, 1826
BssAI	RCCGGY	3 cut(s)	214, 1249, 1826
BssECI	CCNNGG	4 cut(s)	16, 95, 2499, 2968
BssMI	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
BssNI	GRCGYC	1 cut(s)	183
BssSI	CACGAG	1 cut(s)	269
BssT1I	CCWWGG	2 cut(s)	16, 2499
Bst2BI	CACGAG	1 cut(s)	269
Bst2UI	CCWGG	5 cut(s)	687, 1143, 2865, 2969, 3008
Bst4CI	ACNGT	1 cut(s)	2109
Bst6I	CTCTTC	5 cut(s)	51, 321, 1198, 2385, 2502
BstACI	GRCGYC	1 cut(s)	183
BstAFI	CTTAAG	1 cut(s)	671
BstBAI	YACGTR	1 cut(s)	2360
BstEII	GGTNACC	1 cut(s)	785
BstF5I	GGATG	5 cut(s)	262, 799, 927, 1537, 2830
BstFNI	CGCG	2 cut(s)	80, 225
BstHHI	GCGC	5 cut(s)	82, 225, 1969, 2199, 3025
BstKTI	GATC	8 cut(s)	310, 454, 595, 768, 840, 1099, 1550, 1811
BstMAI	GTCTC	3 cut(s)	1165, 1817, 2862
BstMBI	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
BstMCI	CGRYCG	1 cut(s)	294
BstNI	CCWGG	5 cut(s)	687, 1143, 2865, 2969, 3008
BstNSI	RCATGY	3 cut(s)	411, 911, 1993
BstPI	GGTNACC	1 cut(s)	785
BstSCI	CCNGG	8 cut(s)	178, 288, 685, 789, 1141, 2863, 2967, 3006
BstSFI	CTRYAG	3 cut(s)	1017, 2760, 2907
BstUI	CGCG	2 cut(s)	80, 225
BstV2I	GAAGAC	1 cut(s)	976
BstX2I	RGATCY	2 cut(s)	765, 837
BstXI	CCANNNNNNTGG	2 cut(s)	139, 361
BstYI	RGATCY	2 cut(s)	765, 837
Bsu15I	ATCGAT	1 cut(s)	595
BsuI	GTATCC	2 cut(s)	2740, 2746
BsuRI	GGCC	6 cut(s)	143, 214, 753, 2372, 2955, 2967
BsuTUI	ATCGAT	1 cut(s)	595
BtgZI	GCGATG	2 cut(s)	2822, 2967
BtrI	CACGTC	1 cut(s)	692
BtsCI	GGATG	5 cut(s)	262, 799, 927, 1537, 2830
BtsIMutI	CAGTG	7 cut(s)	381, 759, 915, 975, 1262, 1701, 2100
BveI	ACCTGC	2 cut(s)	836, 1988
CfoI	GCGC	5 cut(s)	82, 225, 1969, 2199, 3025
Cfr10I	RCCGGY	3 cut(s)	214, 1249, 1826
Cfr13I	GGNCC	9 cut(s)	373, 743, 752, 1134, 1672, 2098, 2278, 2340, 2918
ClaI	ATCGAT	1 cut(s)	595
CseI	GACGC	2 cut(s)	172, 2564
Csp6I	GTAC	6 cut(s)	274, 695, 947, 1247, 1956, 2861
CspAI	ACCGGT	1 cut(s)	1249
CspCI	CAANNNNNGTGG	2 cut(s)	595, 630
CviQI	GTAC	6 cut(s)	274, 695, 947, 1247, 1956, 2861
DpnI	GATC	8 cut(s)	309, 453, 594, 767, 839, 1098, 1549, 1810
DpnII	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
DraI	TTTAAA	1 cut(s)	2542
DrdI	GACNNNNNNGTC	1 cut(s)	980
DriI	GACNNNNNGTC	1 cut(s)	2284
DseDI	GACNNNNNNGTC	1 cut(s)	980
EaeI	YGGCCR	3 cut(s)	141, 212, 2965
Eam1104I	CTCTTC	5 cut(s)	51, 321, 1198, 2385, 2502
Eam1105I	GACNNNNNGTC	1 cut(s)	2284
EarI	CTCTTC	5 cut(s)	51, 321, 1198, 2385, 2502
EciI	GGCGGA	2 cut(s)	386, 2708
Eco130I	CCWWGG	2 cut(s)	16, 2499
Eco147I	AGGCCT	1 cut(s)	2372
Eco24I	GRGCYC	1 cut(s)	1027
Eco31I	GGTCTC	1 cut(s)	1817
Eco32I	GATATC	1 cut(s)	2590
Eco47I	GGWCC	8 cut(s)	373, 743, 1134, 1672, 2098, 2278, 2340, 2918
Eco57I	CTGAAG	6 cut(s)	235, 935, 1620, 2409, 2526, 2597
Eco72I	CACGTG	1 cut(s)	2360
Eco88I	CYCGRG	4 cut(s)	242, 833, 1539, 2519
Eco91I	GGTNACC	1 cut(s)	785
EcoO109I	RGGNCCY	4 cut(s)	1134, 2278, 2340, 2918
EcoO65I	GGTNACC	1 cut(s)	785
EcoRII	CCWGG	5 cut(s)	685, 1141, 2863, 2967, 3006
EcoRV	GATATC	1 cut(s)	2590
EcoT14I	CCWWGG	2 cut(s)	16, 2499
EcoT22I	ATGCAT	1 cut(s)	2983
EcoT38I	GRGCYC	1 cut(s)	1027
ErhI	CCWWGG	2 cut(s)	16, 2499
Esp3I	CGTCTC	1 cut(s)	1165
FalI	AAGNNNNNCTT	6 cut(s)	704, 736, 925, 957, 2940, 2972
FauI	CCCGC	1 cut(s)	222
FblI	GTMKAC	1 cut(s)	1763
FokI	GGATG	5 cut(s)	269, 806, 934, 1544, 2837
FriOI	GRGCYC	1 cut(s)	1027
FspBI	CTAG	3 cut(s)	206, 402, 2552
FspI	TGCGCA	1 cut(s)	3024
GlaI	GCGC	5 cut(s)	81, 224, 1968, 2198, 3024
GsaI	CCCAGC	1 cut(s)	635
GsuI	CTGGAG	2 cut(s)	1914, 2877
HaeIII	GGCC	6 cut(s)	143, 214, 753, 2372, 2955, 2967
HapII	CCGG	7 cut(s)	180, 215, 290, 790, 1250, 1283, 1827
HgaI	GACGC	2 cut(s)	172, 2564
HhaI	GCGC	5 cut(s)	82, 225, 1969, 2199, 3025
Hin1I	GRCGYC	1 cut(s)	183
Hin6I	GCGC	5 cut(s)	80, 223, 1967, 2197, 3023
HinP1I	GCGC	5 cut(s)	80, 223, 1967, 2197, 3023
HincII	GTYRAC	6 cut(s)	811, 913, 1072, 1942, 2032, 2904
HindII	GTYRAC	6 cut(s)	811, 913, 1072, 1942, 2032, 2904
HindIII	AAGCTT	2 cut(s)	560, 2493
HpaII	CCGG	7 cut(s)	180, 215, 290, 790, 1250, 1283, 1827
HphI	GGTGA	6 cut(s)	115, 632, 746, 1283, 1931, 2422
Hpy99I	CGWCG	4 cut(s)	160, 163, 188, 1188
HpyAV	CCTTC	8 cut(s)	247, 1220, 1568, 1897, 2032, 2409, 2788, 2925
HpyCH4III	ACNGT	1 cut(s)	2109
HpyCH4IV	ACGT	5 cut(s)	61, 155, 691, 1174, 2359
HpySE526I	ACGT	5 cut(s)	61, 155, 691, 1174, 2359
Hsp92I	GRCGYC	1 cut(s)	183
HspAI	GCGC	5 cut(s)	80, 223, 1967, 2197, 3023
KflI	GGGWCCC	3 cut(s)	1134, 2340, 2918
Kpn2I	TCCGGA	1 cut(s)	1282
KroI	GCCGGC	1 cut(s)	214
KroNI	GCCGGC	1 cut(s)	216
Kzo9I	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
LmnI	GCTCC	3 cut(s)	122, 646, 1285
MaeI	CTAG	3 cut(s)	206, 402, 2552
MaeII	ACGT	5 cut(s)	61, 155, 691, 1174, 2359
MaeIII	GTNAC	5 cut(s)	62, 84, 785, 1919, 2428
MalI	GATC	8 cut(s)	309, 453, 594, 767, 839, 1098, 1549, 1810
MboI	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
MfeI	CAATTG	2 cut(s)	2127, 2882
MflI	RGATCY	2 cut(s)	765, 837
MhlI	GDGCHC	1 cut(s)	1027
MlsI	TGGCCA	1 cut(s)	2967
MluNI	TGGCCA	1 cut(s)	2967
MlyI	GAGTC	3 cut(s)	1345, 1462, 1769
MmeI	TCCRAC	2 cut(s)	335, 1027
Mox20I	TGGCCA	1 cut(s)	2967
Mph1103I	ATGCAT	1 cut(s)	2983
MroI	TCCGGA	1 cut(s)	1282
MroNI	GCCGGC	1 cut(s)	214
MroXI	GAANNNNTTC	2 cut(s)	1111, 1164
MscI	TGGCCA	1 cut(s)	2967
MseI	TTAA	9 cut(s)	348, 672, 888, 1359, 1689, 2169, 2210, 2457, 2541
MslI	CAYNNNNRTG	6 cut(s)	920, 1589, 1623, 1680, 1988, 2739
Msp20I	TGGCCA	1 cut(s)	2967
MspA1I	CMGCKG	1 cut(s)	2573
MspCI	CTTAAG	1 cut(s)	671
MspI	CCGG	7 cut(s)	180, 215, 290, 790, 1250, 1283, 1827
MspR9I	CCNGG	8 cut(s)	180, 290, 687, 791, 1143, 2865, 2969, 3008
MunI	CAATTG	2 cut(s)	2127, 2882
Mva1269I	GAATGC	3 cut(s)	1561, 1600, 2805
MvaI	CCWGG	5 cut(s)	687, 1143, 2865, 2969, 3008
MvnI	CGCG	2 cut(s)	80, 225
NaeI	GCCGGC	1 cut(s)	216
NciI	CCSGG	3 cut(s)	180, 290, 791
NdeII	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
NgoMIV	GCCGGC	1 cut(s)	214
NmuCI	GTSAC	3 cut(s)	84, 1919, 2428
NsbI	TGCGCA	1 cut(s)	3024
NsiI	ATGCAT	1 cut(s)	2983
NspI	RCATGY	3 cut(s)	411, 911, 1993
OliI	CACNNNNGTG	1 cut(s)	1623
PaeI	GCATGC	1 cut(s)	411
PaeR7I	CTCGAG	4 cut(s)	242, 833, 1539, 2519
PceI	AGGCCT	1 cut(s)	2372
PciI	ACATGT	1 cut(s)	1989
PcsI	WCGNNNNNNNCGW	3 cut(s)	374, 1180, 1192
PctI	GAATGC	3 cut(s)	1561, 1600, 2805
PdiI	GCCGGC	1 cut(s)	216
PdmI	GAANNNNTTC	2 cut(s)	1111, 1164
PfeI	GAWTC	8 cut(s)	12, 100, 106, 112, 188, 2123, 2409, 2468
PflMI	CCANNNNNTGG	2 cut(s)	549, 1037
PinAI	ACCGGT	1 cut(s)	1249
PleI	GAGTC	3 cut(s)	1345, 1462, 1768
PmaCI	CACGTG	1 cut(s)	2360
PmlI	CACGTG	1 cut(s)	2360
PpsI	GAGTC	3 cut(s)	1345, 1462, 1768
Ppu21I	YACGTR	1 cut(s)	2360
PpuMI	RGGWCCY	4 cut(s)	1134, 2278, 2340, 2918
PscI	ACATGT	1 cut(s)	1989
PsiI	TTATAA	1 cut(s)	579
Psp5II	RGGWCCY	4 cut(s)	1134, 2278, 2340, 2918
Psp6I	CCWGG	5 cut(s)	685, 1141, 2863, 2967, 3006
PspCI	CACGTG	1 cut(s)	2360
PspEI	GGTNACC	1 cut(s)	785
PspFI	CCCAGC	1 cut(s)	631
PspGI	CCWGG	5 cut(s)	685, 1141, 2863, 2967, 3006
PspPI	GGNCC	9 cut(s)	373, 743, 752, 1134, 1672, 2098, 2278, 2340, 2918
PspPPI	RGGWCCY	4 cut(s)	1134, 2278, 2340, 2918
PspXI	VCTCGAGB	1 cut(s)	242
PstI	CTGCAG	2 cut(s)	2764, 2911
PsuI	RGATCY	2 cut(s)	765, 837
RsaI	GTAC	6 cut(s)	275, 696, 948, 1248, 1957, 2862
RsaNI	GTAC	6 cut(s)	274, 695, 947, 1247, 1956, 2861
RseI	CAYNNNNRTG	6 cut(s)	920, 1589, 1623, 1680, 1988, 2739
SaqAI	TTAA	9 cut(s)	348, 672, 888, 1359, 1689, 2169, 2210, 2457, 2541
Sau3AI	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
Sau96I	GGNCC	9 cut(s)	373, 743, 752, 1134, 1672, 2098, 2278, 2340, 2918
ScaI	AGTACT	1 cut(s)	275
SchI	GAGTC	3 cut(s)	1345, 1462, 1769
ScrFI	CCNGG	8 cut(s)	180, 290, 687, 791, 1143, 2865, 2969, 3008
SduI	GDGCHC	1 cut(s)	1027
SfcI	CTRYAG	3 cut(s)	1017, 2760, 2907
Sfr274I	CTCGAG	4 cut(s)	242, 833, 1539, 2519
SinI	GGWCC	8 cut(s)	373, 743, 1134, 1672, 2098, 2278, 2340, 2918
SlaI	CTCGAG	4 cut(s)	242, 833, 1539, 2519
SmiMI	CAYNNNNRTG	6 cut(s)	920, 1589, 1623, 1680, 1988, 2739
SmlI	CTYRAG	8 cut(s)	242, 671, 833, 1221, 1389, 1539, 2474, 2519
SmoI	CTYRAG	8 cut(s)	242, 671, 833, 1221, 1389, 1539, 2474, 2519
SphI	GCATGC	1 cut(s)	411
SseBI	AGGCCT	1 cut(s)	2372
SspMI	CTAG	3 cut(s)	206, 402, 2552
StuI	AGGCCT	1 cut(s)	2372
StyD4I	CCNGG	8 cut(s)	178, 288, 685, 789, 1141, 2863, 2967, 3006
StyI	CCWWGG	2 cut(s)	16, 2499
TaaI	ACNGT	1 cut(s)	2109
TaiI	ACGT	5 cut(s)	64, 158, 694, 1177, 2362
TaqI	TCGA	9 cut(s)	186, 243, 456, 495, 595, 834, 1186, 1540, 2520
TatI	WGTACW	3 cut(s)	273, 694, 946
TauI	GCSGC	4 cut(s)	146, 753, 1262, 2576
TfiI	GAWTC	8 cut(s)	12, 100, 106, 112, 188, 2123, 2409, 2468
Tru1I	TTAA	9 cut(s)	348, 672, 888, 1359, 1689, 2169, 2210, 2457, 2541
Tru9I	TTAA	9 cut(s)	348, 672, 888, 1359, 1689, 2169, 2210, 2457, 2541
TscAI	CASTG	7 cut(s)	388, 766, 922, 982, 1269, 1708, 2100
TseFI	GTSAC	3 cut(s)	84, 1919, 2428
Tsp45I	GTSAC	3 cut(s)	84, 1919, 2428
TspDTI	ATGAA	9 cut(s)	353, 666, 976, 1104, 1314, 1602, 1607, 1692, 2841
TspGWI	ACGGA	2 cut(s)	1172, 2837
TspRI	CASTG	7 cut(s)	388, 766, 922, 982, 1269, 1708, 2100
Van91I	CCANNNNNTGG	2 cut(s)	549, 1037
Vha464I	CTTAAG	1 cut(s)	671
VpaK11BI	GGWCC	8 cut(s)	373, 743, 1134, 1672, 2098, 2278, 2340, 2918
XapI	RAATTY	5 cut(s)	1373, 1854, 2327, 2565, 2656
XceI	RCATGY	3 cut(s)	411, 911, 1993
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2741
XhoI	CTCGAG	4 cut(s)	242, 833, 1539, 2519
XmiI	GTMKAC	1 cut(s)	1763
XmnI	GAANNNNTTC	2 cut(s)	1111, 1164
XspI	CTAG	3 cut(s)	206, 402, 2552
ZrmI	AGTACT	1 cut(s)	275
Zsp2I	ATGCAT	1 cut(s)	2983

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.