Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0010393

Physical Location & Seq

Genomic Coordinates Forward (+)

66650621 .. 66658688

Gene Structure 8068 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ41403

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3030 bp

ATGAGTGGATTCCTTGGGAAAGGAGTGATAGCGTTCAACCCTCTTCTTCTACTCGGACGTTACCCTTTCTCTTTCGCGCCGTCACACCTCCCTCGGAATCGGAATCGGAATCGCTCCCTCACCACCGCCAAGCACTTGGCTTCAATCGCAACGTCGTCGTTTCGTCCCAAGATGCCCGGCGTCGAATCTCAGGGAGGAGGACTAGAGTGGCCGGCACAGCGCGTGCGGGACACCTTCCTCGAGTTCTTCAGGGATGAGAAGAAACACGAGTACTGGAAGTCAAGCCCGGTCGTCCCCCACAACGACCCAACTCTTCTCTTTGCTAATGCTGGAATGAACCAGTTTAAGCCCATTTTTTTGGGGACGGCGGACCCGAATACTGGGCTAAGCAAGCTGACTAGAGCATGCAACACGCAGAAATGTATAAGGGCAGGAGGGAAGCATAATGATCTCGAAGATGTAGGGAAAGACACCTACCATCACACTTTTTTCGAGATGCTTGGTAATTGGTCGTTCGGGGACTATTTCAAGAGTGAAGCCATTGAATGGGCTTGGAAGCTTCTCACTCAGGCAAGCAGATATCGATTTTATGCCACTTATTTTGGTGGTGATGAGAAGCTGGGTCTTGCTCCTGATTATGAAGCTAGAGATTTATGGCTTAAGTTTCTACCGCCTGGACGTGTACTGCCTTTTGGCTGTAAGGATAACTTTTGGGAGATGGGTGATACTGGTCCTTGCGGCCCTTGCACTGAGATCCATTATGATAGAGTTGGTAACCGGGATGCTGCATCATTAGTCAACTACGATGACCCTACCTGCCTCGAGATCTGGAACCTTGTCTTTATTCAGTTCAATAGGGAAAGTGATGGCTCTCTTAAACCATTGCCTGCTAAGCATGTTGACACTGGGATGGGCTTTGAAAGATTGACTTCTGTACTTCAGAACAAGATGAGCAATTATGACACTGATGTCTTCATGCCTATATTTGATGCTATTCAGCAGGCTACAGGGGCTCGCCCATATTCTGGGAAAGTTGGAGATGATGATACAGACAAAGTTGACATGGCTTACAGGGTTGTTGCAGATCATATAAGAACTCTTTCATTTGCCATTGCTGATGGGTCCCGTCCAGGCAATGATGGTCGTGAATATGTTCTGAGACGTATTCTCCGTCGAGCTGTCCGATATGGAAGAGAAGTGCTAAAAGCTCAAGAAGGATTTTTCAATGGGCTTGTACCGGTGTTGGCGGCACTGATGGGTGATGTATTTCCGGAGCTAAAACAACATGAAGCAGGCATTAGGGAGATAATCAAAGAGGAGGAAGAAGCATTTGGCAAGACTCTAATTAAGGGAATAGAAAAATTCAAAAAGGCAGCTCAAGATGTTCAAGGCAAAACATTTAGTGGGCAGGAGGCTTTTGTCTTGTGGGACACATTTGGGTTCCCGTTAGATTTGACTCAGTTGATGGCAGAGGAACAGGGATTAGAAGTTGATGTTGAGGGTTTCAATGTCGCTATGGATGAGGCTCGAGAAAGATCAAGGAATGCTCAAAATAAGGAAGAAGGTGGTGCCATTGTTATGAATGCTGAAGCTACTGCTTCATTGCACAAGAGTGGAATTGCTGCAACAGACGACAGCTTCAAATATATTTGGTTTCAGGACCATGAAAGTGTAATTAAAGCAATTTACACTGGTTCAGAGTTTATGGATAATGTTGCTGCTGGCAGCTTAGTTGGTGTTATTTTGGAGTCTACAAGTTTCTATGCAGAGCAAGGTGGACAGATATTTGATACTGGATCACTAAAAGGAGAGACCGGCTCATTTCAAGTCCGCAATGTTCAAATTTTTGGAGGTTTTGTTGTCCACATTGGTTCCTTCTCTGGAGAGCATGGCAAACTCTCTGTGGGTGACAAAGTGGTTTGTAAGGTTGACTATAAAAGGCGTACACTCATTGCGCCTAACCATACTTGCACACACATGTTGAACTTTGCTCTGAGGGAAGTTCTTGGCAGTCATGTTGACCAGAAGGGTTCCATTGTTCTTCCTGAAAAATTGAGATTTGATTTTTCTCATGGTAAGCCGGTGGACCCTGAACAGTTGAGAAGAATTGAATCAATTGTGAATGAGCAAATAAAAGCTGAATTAGATGTTTCTGTTAAGGAGGTAGCCCTGGTTGAAGCTGAGCGCATCAATGGTTTAAGGGCTGTATTTGGAGAGGTCTATCCTGACCCAGTAAGAGTTGTGGCTGTTGGGCGAAAAGTTGAGGACCTCCTGTCTGACCCTAAAAATGAGGAATGGTCATCAATATCAACAGAATTTTGTGGAGGGACCCATATAACAAACACACGTGAAGCTGAGGCCTTTGCCCTTTTATCTGAAGAGGGAACTGCTAAGGGAATCCGAAGGATAACTGCTGTCACAACTAATGCTGCTTTTGATGCAATTAAATTGGCAGATTCACTTGAGCAGGAAGTAATTGAAGCTTCCAAGGCTGAAGAGAGCTTGCTCGAGAAGAAGATAGCTTCCTTTAAATCTCGCCTAGACGCAGCACAAATTCCAGCGGCTAAGAAAGCTGATATCAGGGACAAGATTGCCCAACTTCAGAACAAAGTGAGAAAGGCACAGAAGAAGATTGCAGAGCAAAATTTACAGAAAGCTGTCAAGGTTGCAAGGGAGATGGCGGAAGTTGCTGCATCAGATGGGAAGTCTTTCTGTGTATCCCATGTTGATGTTGGTTTGGATACTGCAGCAGTTCGTGAAGCAGTTTGTAAAGTCATAGAGAAGGGAATGCCTGCGATGGTTTTTAGCATGGATGAAACAACAAATAAGGCTGTGGTGTGTGCTGGAGTACCAGGAGACAAAGGCAAGCAATTGGAGGTGTCAGAGTGGTTGACTGCAGCTATGGGTCCCCTGAAAGGAAGGGGTGGTAAAGGAAAAGGTGGCCTTGCTACTGGCCAGGGAACAGATGCATCTCATCTAAACGAGGCAATAGATCTGGCAACCACTTTTGCGCAGATGAAATTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1009

Amino Acids

111.31

Weight (kDa)

6.02

Isoelectric Point (pI)

33.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	75 - 647	1.4e-213	tRNA synthetases class II (A)
tRNA_SAD	PF07973	745 - 803	4.9e-12	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	902 - 1004	2e-09	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50200 AT1G50200
fragaria_vesca	FvH4_4g36780 FvH4_4g36780
malus_domestica	MD13G1007000.v1.1 MD16G1000800.v1.1
prunus_persica	Prupe.1G345900_v2.0.a1
pyrus_communis	pycom13g00590 pycom16g00460
rosa_chinensis	RchiOBHm_Chr4g0446511
rosa_laevigata	RLG00000005653
rosa_multiflora	Rmu_co8401425.1_g000001 Rmu_sc0005526.1_g000002 Rmu_sc0009884.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386930
rosa_rugosa	Rorug04G0372100 Rorug04G0372200
rosa_samantha	Rh4BG442800 Rh4CG460300 Rh4DG441500
rosa_wichuraiana	Rw4G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	968
Acc16I	TGCGCA	1 cut(s)	3012
Acc36I	ACCTGC	1 cut(s)	824
AccB1I	GGYRCC	1 cut(s)	1568
AccB7I	CCANNNNNTGG	2 cut(s)	546, 1025
AccI	GTMKAC	1 cut(s)	1751
AccII	CGCG	2 cut(s)	77, 222
AccIII	TCCGGA	1 cut(s)	1270
AciI	CCGC	9 cut(s)	126, 226, 368, 671, 738, 1247, 1831, 2561, 2681
AclWI	GGATC	2 cut(s)	748, 1804
AcoI	YGGCCR	2 cut(s)	209, 2953
AcsI	RAATTY	5 cut(s)	1361, 1842, 2315, 2553, 2644
AcuI	CTGAAG	6 cut(s)	232, 923, 1608, 2397, 2514, 2585
AcvI	CACGTG	1 cut(s)	2348
AcyI	GRCGYC	1 cut(s)	180
AfaI	GTAC	6 cut(s)	272, 684, 936, 1236, 1945, 2850
AfiI	CCNNNNNNNGG	4 cut(s)	380, 546, 1025, 2915
AflII	CTTAAG	1 cut(s)	659
AflIII	ACRYGT	3 cut(s)	679, 1977, 2345
AgeI	ACCGGT	1 cut(s)	1237
AhdI	GACNNNNNGTC	1 cut(s)	2272
AjiI	CACGTC	1 cut(s)	680
AjnI	CCWGG	5 cut(s)	673, 1129, 2169, 2851, 2955
AjuI	GAANNNNNNNTTGG	2 cut(s)	1314, 1346
AleI	CACNNNNGTG	1 cut(s)	1611
Alw26I	GTCTC	3 cut(s)	1153, 1805, 2850
AlwI	GGATC	2 cut(s)	748, 1804
Ama87I	CYCGRG	4 cut(s)	239, 821, 1527, 2507
Aor13HI	TCCGGA	1 cut(s)	1270
AoxI	GGCC	5 cut(s)	209, 739, 2358, 2941, 2953
ApoI	RAATTY	5 cut(s)	1361, 1842, 2315, 2553, 2644
AsiGI	ACCGGT	1 cut(s)	1237
Asp700I	GAANNNNTTC	3 cut(s)	1099, 1152, 2708
AspLEI	GCGC	5 cut(s)	79, 222, 1957, 2187, 3013
AspS9I	GGNCC	9 cut(s)	370, 731, 740, 1122, 1660, 2086, 2266, 2328, 2906
AsuC2I	CCSGG	3 cut(s)	177, 287, 779
AsuHPI	GGTGA	5 cut(s)	112, 620, 734, 1271, 1919
AvaI	CYCGRG	4 cut(s)	239, 821, 1527, 2507
AvaII	GGWCC	8 cut(s)	370, 731, 1122, 1660, 2086, 2266, 2328, 2906
BalI	TGGCCA	1 cut(s)	2955
BanI	GGYRCC	1 cut(s)	1568
BanII	GRGCYC	1 cut(s)	1015
BauI	CACGAG	1 cut(s)	266
BbrPI	CACGTG	1 cut(s)	2348
BbsI	GAAGAC	1 cut(s)	964
BbvCI	CCTCAGC	1 cut(s)	2355
BceAI	ACGGC	2 cut(s)	64, 381
BcgI	CGANNNNNNTGC	2 cut(s)	138, 172
BciT130I	CCWGG	5 cut(s)	675, 1131, 2171, 2853, 2957
BciVI	GTATCC	2 cut(s)	2728, 2734
BcnI	CCSGG	3 cut(s)	177, 287, 779
BcoDI	GTCTC	3 cut(s)	1153, 1805, 2850
BfaI	CTAG	4 cut(s)	203, 399, 645, 2540
BfmI	CTRYAG	3 cut(s)	1005, 2745, 2895
BfrI	CTTAAG	1 cut(s)	659
BfuAI	ACCTGC	1 cut(s)	824
BfuI	GTATCC	2 cut(s)	2728, 2734
BglII	AGATCT	2 cut(s)	825, 2992
BlpI	GCTNAGC	3 cut(s)	386, 891, 2181
BmcAI	AGTACT	1 cut(s)	272
Bme1390I	CCNGG	8 cut(s)	177, 287, 675, 779, 1131, 2171, 2853, 2957
Bme18I	GGWCC	8 cut(s)	370, 731, 1122, 1660, 2086, 2266, 2328, 2906
BmeRI	GACNNNNNGTC	1 cut(s)	2272
BmeT110I	CYCGRG	4 cut(s)	239, 821, 1527, 2507
BmgBI	CACGTC	1 cut(s)	680
BmgT120I	GGNCC	9 cut(s)	370, 731, 740, 1122, 1660, 2086, 2266, 2328, 2906
BmrFI	CCNGG	8 cut(s)	177, 287, 675, 779, 1131, 2171, 2853, 2957
BmrI	ACTGGG	3 cut(s)	390, 915, 2225
BmuI	ACTGGG	3 cut(s)	390, 915, 2225
BpiI	GAAGAC	1 cut(s)	964
BplI	GAGNNNNNCTC	2 cut(s)	1802, 1834
BpmI	CTGGAG	2 cut(s)	1902, 2865
Bpu10I	CCTNAGC	2 cut(s)	2355, 2391
Bpu1102I	GCTNAGC	3 cut(s)	386, 891, 2181
BpuEI	CTTGAG	3 cut(s)	1194, 1362, 2483
BpuMI	CCSGG	3 cut(s)	177, 287, 779
Bsa29I	ATCGAT	1 cut(s)	583
BsaAI	YACGTR	1 cut(s)	2348
BsaHI	GRCGYC	1 cut(s)	180
BsaI	GGTCTC	1 cut(s)	1805
BsaJI	CCNNGG	5 cut(s)	13, 92, 2169, 2487, 2956
BsaWI	WCCGGW	2 cut(s)	1237, 1270
BsaXI	ACNNNNNCTCC	4 cut(s)	1152, 1182, 1842, 1872
Bsc4I	CCNNNNNNNGG	4 cut(s)	380, 546, 1025, 2915
Bse118I	RCCGGY	4 cut(s)	211, 1237, 1814, 2080
Bse1I	ACTGG	9 cut(s)	278, 340, 385, 733, 910, 1696, 1798, 2231, 2956
Bse3DI	GCAATG	6 cut(s)	881, 1110, 1141, 1601, 1840, 1950
BseAI	TCCGGA	1 cut(s)	1270
BseBI	CCWGG	5 cut(s)	675, 1131, 2171, 2853, 2957
BseCI	ATCGAT	1 cut(s)	583
BseDI	CCNNGG	5 cut(s)	13, 92, 2169, 2487, 2956
BseGI	GGATG	5 cut(s)	259, 787, 915, 1525, 2818
BseLI	CCNNNNNNNGG	4 cut(s)	380, 546, 1025, 2915
BseMI	GCAATG	6 cut(s)	881, 1110, 1141, 1601, 1840, 1950
BseMII	CTCAG	8 cut(s)	203, 581, 741, 1148, 1472, 1985, 2172, 2346
BseNI	ACTGG	9 cut(s)	278, 340, 385, 733, 910, 1696, 1798, 2231, 2956
BseRI	GAGGAG	2 cut(s)	210, 1331
BseYI	CCCAGC	1 cut(s)	619
Bsh1236I	CGCG	2 cut(s)	77, 222
Bsh1285I	CGRYCG	1 cut(s)	291
BshFI	GGCC	5 cut(s)	211, 741, 2360, 2943, 2955
BshNI	GGYRCC	1 cut(s)	1568
BshTI	ACCGGT	1 cut(s)	1237
BshVI	ATCGAT	1 cut(s)	583
BsiEI	CGRYCG	1 cut(s)	291
BsiHKCI	CYCGRG	4 cut(s)	239, 821, 1527, 2507
BsiSI	CCGG	8 cut(s)	177, 212, 287, 778, 1238, 1271, 1815, 2081
BslI	CCNNNNNNNGG	4 cut(s)	380, 546, 1025, 2915
BsmAI	GTCTC	3 cut(s)	1153, 1805, 2850
BsmBI	CGTCTC	1 cut(s)	1153
BsmI	GAATGC	3 cut(s)	1549, 1588, 2793
BsnI	GGCC	5 cut(s)	211, 741, 2360, 2943, 2955
Bso31I	GGTCTC	1 cut(s)	1805
BsoBI	CYCGRG	4 cut(s)	239, 821, 1527, 2507
Bsp1286I	GDGCHC	1 cut(s)	1015
Bsp13I	TCCGGA	1 cut(s)	1270
Bsp143I	GATC	7 cut(s)	448, 753, 825, 1084, 1535, 1796, 2992
Bsp1720I	GCTNAGC	3 cut(s)	386, 891, 2181
BspACI	CCGC	9 cut(s)	126, 226, 368, 671, 738, 1247, 1831, 2561, 2681
BspANI	GGCC	5 cut(s)	211, 741, 2360, 2943, 2955
BspCNI	CTCAG	8 cut(s)	202, 580, 742, 1149, 1471, 1986, 2173, 2347
BspDI	ATCGAT	1 cut(s)	583
BspEI	TCCGGA	1 cut(s)	1270
BspFNI	CGCG	2 cut(s)	77, 222
BspMAI	CTGCAG	2 cut(s)	2749, 2899
BspMI	ACCTGC	1 cut(s)	824
BspPI	GGATC	2 cut(s)	748, 1804
BspT107I	GGYRCC	1 cut(s)	1568
BspTI	CTTAAG	1 cut(s)	659
BspTNI	GGTCTC	1 cut(s)	1805
BsrDI	GCAATG	6 cut(s)	881, 1110, 1141, 1601, 1840, 1950
BsrFI	RCCGGY	4 cut(s)	211, 1237, 1814, 2080
BsrI	ACTGG	9 cut(s)	278, 340, 385, 733, 910, 1696, 1798, 2231, 2956
BssAI	RCCGGY	4 cut(s)	211, 1237, 1814, 2080
BssECI	CCNNGG	5 cut(s)	13, 92, 2169, 2487, 2956
BssMI	GATC	7 cut(s)	448, 753, 825, 1084, 1535, 1796, 2992
BssNI	GRCGYC	1 cut(s)	180
BssSI	CACGAG	1 cut(s)	266
BssT1I	CCWWGG	2 cut(s)	13, 2487
Bst2BI	CACGAG	1 cut(s)	266
Bst2UI	CCWGG	5 cut(s)	675, 1131, 2171, 2853, 2957
Bst4CI	ACNGT	1 cut(s)	2097
Bst6I	CTCTTC	5 cut(s)	48, 318, 1186, 2373, 2490
BstACI	GRCGYC	1 cut(s)	180
BstAFI	CTTAAG	1 cut(s)	659
BstBAI	YACGTR	1 cut(s)	2348
BstEII	GGTNACC	1 cut(s)	773
BstF5I	GGATG	5 cut(s)	259, 787, 915, 1525, 2818
BstFNI	CGCG	2 cut(s)	77, 222
BstHHI	GCGC	5 cut(s)	79, 222, 1957, 2187, 3013
BstKTI	GATC	7 cut(s)	451, 756, 828, 1087, 1538, 1799, 2995
BstMAI	GTCTC	3 cut(s)	1153, 1805, 2850
BstMBI	GATC	7 cut(s)	448, 753, 825, 1084, 1535, 1796, 2992
BstMCI	CGRYCG	1 cut(s)	291
BstNI	CCWGG	5 cut(s)	675, 1131, 2171, 2853, 2957
BstNSI	RCATGY	3 cut(s)	408, 899, 1981
BstPI	GGTNACC	1 cut(s)	773
BstSCI	CCNGG	8 cut(s)	175, 285, 673, 777, 1129, 2169, 2851, 2955
BstSFI	CTRYAG	3 cut(s)	1005, 2745, 2895
BstUI	CGCG	2 cut(s)	77, 222
BstV2I	GAAGAC	1 cut(s)	964
BstX2I	RGATCY	3 cut(s)	753, 825, 2992
BstXI	CCANNNNNNTGG	2 cut(s)	136, 358
BstYI	RGATCY	3 cut(s)	753, 825, 2992
Bsu15I	ATCGAT	1 cut(s)	583
BsuI	GTATCC	2 cut(s)	2728, 2734
BsuRI	GGCC	5 cut(s)	211, 741, 2360, 2943, 2955
BsuTUI	ATCGAT	1 cut(s)	583
BtgZI	GCGATG	1 cut(s)	2810
BtrI	CACGTC	1 cut(s)	680
BtsCI	GGATG	5 cut(s)	259, 787, 915, 1525, 2818
BtsIMutI	CAGTG	5 cut(s)	747, 903, 963, 1250, 1689
BveI	ACCTGC	1 cut(s)	824
CfoI	GCGC	5 cut(s)	79, 222, 1957, 2187, 3013
Cfr10I	RCCGGY	4 cut(s)	211, 1237, 1814, 2080
Cfr13I	GGNCC	9 cut(s)	370, 731, 740, 1122, 1660, 2086, 2266, 2328, 2906
ClaI	ATCGAT	1 cut(s)	583
CseI	GACGC	2 cut(s)	169, 2552
Csp6I	GTAC	6 cut(s)	271, 683, 935, 1235, 1944, 2849
CspAI	ACCGGT	1 cut(s)	1237
CspCI	CAANNNNNGTGG	2 cut(s)	583, 618
CviQI	GTAC	6 cut(s)	271, 683, 935, 1235, 1944, 2849
DpnI	GATC	7 cut(s)	450, 755, 827, 1086, 1537, 1798, 2994
DpnII	GATC	7 cut(s)	448, 753, 825, 1084, 1535, 1796, 2992
DraI	TTTAAA	1 cut(s)	2530
DrdI	GACNNNNNNGTC	1 cut(s)	968
DriI	GACNNNNNGTC	1 cut(s)	2272
DseDI	GACNNNNNNGTC	1 cut(s)	968
EaeI	YGGCCR	2 cut(s)	209, 2953
Eam1104I	CTCTTC	5 cut(s)	48, 318, 1186, 2373, 2490
Eam1105I	GACNNNNNGTC	1 cut(s)	2272
EarI	CTCTTC	5 cut(s)	48, 318, 1186, 2373, 2490
EciI	GGCGGA	2 cut(s)	383, 2696
Eco130I	CCWWGG	2 cut(s)	13, 2487
Eco147I	AGGCCT	1 cut(s)	2360
Eco24I	GRGCYC	1 cut(s)	1015
Eco31I	GGTCTC	1 cut(s)	1805
Eco32I	GATATC	2 cut(s)	581, 2578
Eco47I	GGWCC	8 cut(s)	370, 731, 1122, 1660, 2086, 2266, 2328, 2906
Eco57I	CTGAAG	6 cut(s)	232, 923, 1608, 2397, 2514, 2585
Eco72I	CACGTG	1 cut(s)	2348
Eco88I	CYCGRG	4 cut(s)	239, 821, 1527, 2507
Eco91I	GGTNACC	1 cut(s)	773
EcoO109I	RGGNCCY	4 cut(s)	1122, 2266, 2328, 2906
EcoO65I	GGTNACC	1 cut(s)	773
EcoRII	CCWGG	5 cut(s)	673, 1129, 2169, 2851, 2955
EcoRV	GATATC	2 cut(s)	581, 2578
EcoT14I	CCWWGG	2 cut(s)	13, 2487
EcoT22I	ATGCAT	1 cut(s)	2971
EcoT38I	GRGCYC	1 cut(s)	1015
ErhI	CCWWGG	2 cut(s)	13, 2487
Esp3I	CGTCTC	1 cut(s)	1153
FalI	AAGNNNNNCTT	6 cut(s)	692, 724, 913, 945, 2928, 2960
FauI	CCCGC	1 cut(s)	219
FblI	GTMKAC	1 cut(s)	1751
FokI	GGATG	5 cut(s)	266, 794, 922, 1532, 2825
FriOI	GRGCYC	1 cut(s)	1015
FspBI	CTAG	4 cut(s)	203, 399, 645, 2540
FspI	TGCGCA	1 cut(s)	3012
GlaI	GCGC	5 cut(s)	78, 221, 1956, 2186, 3012
GsaI	CCCAGC	1 cut(s)	623
GsuI	CTGGAG	2 cut(s)	1902, 2865
HaeIII	GGCC	5 cut(s)	211, 741, 2360, 2943, 2955
HapII	CCGG	8 cut(s)	177, 212, 287, 778, 1238, 1271, 1815, 2081
HgaI	GACGC	2 cut(s)	169, 2552
HhaI	GCGC	5 cut(s)	79, 222, 1957, 2187, 3013
Hin1I	GRCGYC	1 cut(s)	180
Hin6I	GCGC	5 cut(s)	77, 220, 1955, 2185, 3011
HinP1I	GCGC	5 cut(s)	77, 220, 1955, 2185, 3011
HincII	GTYRAC	6 cut(s)	799, 901, 1060, 1930, 2020, 2892
HindII	GTYRAC	6 cut(s)	799, 901, 1060, 1930, 2020, 2892
HindIII	AAGCTT	2 cut(s)	557, 2481
HpaII	CCGG	8 cut(s)	177, 212, 287, 778, 1238, 1271, 1815, 2081
HphI	GGTGA	5 cut(s)	112, 620, 734, 1271, 1919
Hpy99I	CGWCG	4 cut(s)	157, 160, 185, 1176
HpyAV	CCTTC	8 cut(s)	244, 1208, 1556, 1885, 2020, 2397, 2776, 2913
HpyCH4III	ACNGT	1 cut(s)	2097
HpyCH4IV	ACGT	5 cut(s)	58, 152, 679, 1162, 2347
HpySE526I	ACGT	5 cut(s)	58, 152, 679, 1162, 2347
Hsp92I	GRCGYC	1 cut(s)	180
HspAI	GCGC	5 cut(s)	77, 220, 1955, 2185, 3011
KflI	GGGWCCC	3 cut(s)	1122, 2328, 2906
Kpn2I	TCCGGA	1 cut(s)	1270
KroI	GCCGGC	1 cut(s)	211
KroNI	GCCGGC	1 cut(s)	213
Kzo9I	GATC	7 cut(s)	448, 753, 825, 1084, 1535, 1796, 2992
LmnI	GCTCC	3 cut(s)	119, 634, 1273
MaeI	CTAG	4 cut(s)	203, 399, 645, 2540
MaeII	ACGT	5 cut(s)	58, 152, 679, 1162, 2347
MaeIII	GTNAC	5 cut(s)	59, 81, 773, 1907, 2416
MalI	GATC	7 cut(s)	450, 755, 827, 1086, 1537, 1798, 2994
MboI	GATC	7 cut(s)	448, 753, 825, 1084, 1535, 1796, 2992
MfeI	CAATTG	2 cut(s)	2115, 2870
MflI	RGATCY	3 cut(s)	753, 825, 2992
MhlI	GDGCHC	1 cut(s)	1015
MlsI	TGGCCA	1 cut(s)	2955
MluNI	TGGCCA	1 cut(s)	2955
MlyI	GAGTC	3 cut(s)	1333, 1450, 1757
MmeI	TCCRAC	1 cut(s)	1015
Mox20I	TGGCCA	1 cut(s)	2955
Mph1103I	ATGCAT	1 cut(s)	2971
MroI	TCCGGA	1 cut(s)	1270
MroNI	GCCGGC	1 cut(s)	211
MroXI	GAANNNNTTC	3 cut(s)	1099, 1152, 2708
MscI	TGGCCA	1 cut(s)	2955
MseI	TTAA	9 cut(s)	345, 660, 876, 1347, 1677, 2157, 2198, 2445, 2529
MslI	CAYNNNNRTG	6 cut(s)	908, 1577, 1611, 1668, 1976, 2727
Msp20I	TGGCCA	1 cut(s)	2955
MspA1I	CMGCKG	1 cut(s)	2561
MspCI	CTTAAG	1 cut(s)	659
MspI	CCGG	8 cut(s)	177, 212, 287, 778, 1238, 1271, 1815, 2081
MspR9I	CCNGG	8 cut(s)	177, 287, 675, 779, 1131, 2171, 2853, 2957
MunI	CAATTG	2 cut(s)	2115, 2870
Mva1269I	GAATGC	3 cut(s)	1549, 1588, 2793
MvaI	CCWGG	5 cut(s)	675, 1131, 2171, 2853, 2957
MvnI	CGCG	2 cut(s)	77, 222
NaeI	GCCGGC	1 cut(s)	213
NciI	CCSGG	3 cut(s)	177, 287, 779
NdeII	GATC	7 cut(s)	448, 753, 825, 1084, 1535, 1796, 2992
NgoMIV	GCCGGC	1 cut(s)	211
NmuCI	GTSAC	3 cut(s)	81, 1907, 2416
NsbI	TGCGCA	1 cut(s)	3012
NsiI	ATGCAT	1 cut(s)	2971
NspI	RCATGY	3 cut(s)	408, 899, 1981
OliI	CACNNNNGTG	1 cut(s)	1611
PaeI	GCATGC	1 cut(s)	408
PaeR7I	CTCGAG	4 cut(s)	239, 821, 1527, 2507
PceI	AGGCCT	1 cut(s)	2360
PciI	ACATGT	1 cut(s)	1977
PcsI	WCGNNNNNNNCGW	3 cut(s)	371, 1168, 1180
PctI	GAATGC	3 cut(s)	1549, 1588, 2793
PdiI	GCCGGC	1 cut(s)	213
PdmI	GAANNNNTTC	3 cut(s)	1099, 1152, 2708
PfeI	GAWTC	8 cut(s)	9, 97, 103, 109, 185, 2111, 2397, 2456
PflMI	CCANNNNNTGG	2 cut(s)	546, 1025
PinAI	ACCGGT	1 cut(s)	1237
PleI	GAGTC	3 cut(s)	1333, 1450, 1756
PmaCI	CACGTG	1 cut(s)	2348
PmlI	CACGTG	1 cut(s)	2348
PpsI	GAGTC	3 cut(s)	1333, 1450, 1756
Ppu21I	YACGTR	1 cut(s)	2348
PpuMI	RGGWCCY	4 cut(s)	1122, 2266, 2328, 2906
PscI	ACATGT	1 cut(s)	1977
Psp5II	RGGWCCY	4 cut(s)	1122, 2266, 2328, 2906
Psp6I	CCWGG	5 cut(s)	673, 1129, 2169, 2851, 2955
PspCI	CACGTG	1 cut(s)	2348
PspEI	GGTNACC	1 cut(s)	773
PspFI	CCCAGC	1 cut(s)	619
PspGI	CCWGG	5 cut(s)	673, 1129, 2169, 2851, 2955
PspPI	GGNCC	9 cut(s)	370, 731, 740, 1122, 1660, 2086, 2266, 2328, 2906
PspPPI	RGGWCCY	4 cut(s)	1122, 2266, 2328, 2906
PspXI	VCTCGAGB	1 cut(s)	239
PstI	CTGCAG	2 cut(s)	2749, 2899
PsuI	RGATCY	3 cut(s)	753, 825, 2992
RsaI	GTAC	6 cut(s)	272, 684, 936, 1236, 1945, 2850
RsaNI	GTAC	6 cut(s)	271, 683, 935, 1235, 1944, 2849
RseI	CAYNNNNRTG	6 cut(s)	908, 1577, 1611, 1668, 1976, 2727
SaqAI	TTAA	9 cut(s)	345, 660, 876, 1347, 1677, 2157, 2198, 2445, 2529
Sau3AI	GATC	7 cut(s)	448, 753, 825, 1084, 1535, 1796, 2992
Sau96I	GGNCC	9 cut(s)	370, 731, 740, 1122, 1660, 2086, 2266, 2328, 2906
ScaI	AGTACT	1 cut(s)	272
SchI	GAGTC	3 cut(s)	1333, 1450, 1757
ScrFI	CCNGG	8 cut(s)	177, 287, 675, 779, 1131, 2171, 2853, 2957
SduI	GDGCHC	1 cut(s)	1015
SfcI	CTRYAG	3 cut(s)	1005, 2745, 2895
Sfr274I	CTCGAG	4 cut(s)	239, 821, 1527, 2507
SinI	GGWCC	8 cut(s)	370, 731, 1122, 1660, 2086, 2266, 2328, 2906
SlaI	CTCGAG	4 cut(s)	239, 821, 1527, 2507
SmiMI	CAYNNNNRTG	6 cut(s)	908, 1577, 1611, 1668, 1976, 2727
SmlI	CTYRAG	8 cut(s)	239, 659, 821, 1209, 1377, 1527, 2462, 2507
SmoI	CTYRAG	8 cut(s)	239, 659, 821, 1209, 1377, 1527, 2462, 2507
SphI	GCATGC	1 cut(s)	408
SseBI	AGGCCT	1 cut(s)	2360
SsiI	CCGC	9 cut(s)	126, 226, 368, 671, 738, 1247, 1831, 2561, 2681
SspMI	CTAG	4 cut(s)	203, 399, 645, 2540
StuI	AGGCCT	1 cut(s)	2360
StyD4I	CCNGG	8 cut(s)	175, 285, 673, 777, 1129, 2169, 2851, 2955
StyI	CCWWGG	2 cut(s)	13, 2487
TaaI	ACNGT	1 cut(s)	2097
TaiI	ACGT	5 cut(s)	61, 155, 682, 1165, 2350
TaqI	TCGA	9 cut(s)	183, 240, 453, 492, 583, 822, 1174, 1528, 2508
TatI	WGTACW	3 cut(s)	270, 682, 934
TauI	GCSGC	3 cut(s)	741, 1250, 2564
TfiI	GAWTC	8 cut(s)	9, 97, 103, 109, 185, 2111, 2397, 2456
Tru1I	TTAA	9 cut(s)	345, 660, 876, 1347, 1677, 2157, 2198, 2445, 2529
Tru9I	TTAA	9 cut(s)	345, 660, 876, 1347, 1677, 2157, 2198, 2445, 2529
TscAI	CASTG	5 cut(s)	754, 910, 970, 1257, 1696
TseFI	GTSAC	3 cut(s)	81, 1907, 2416
Tsp45I	GTSAC	3 cut(s)	81, 1907, 2416
TspDTI	ATGAA	9 cut(s)	350, 654, 964, 1092, 1302, 1590, 1595, 1680, 2829
TspGWI	ACGGA	1 cut(s)	1160
TspRI	CASTG	5 cut(s)	754, 910, 970, 1257, 1696
Van91I	CCANNNNNTGG	2 cut(s)	546, 1025
Vha464I	CTTAAG	1 cut(s)	659
VpaK11BI	GGWCC	8 cut(s)	370, 731, 1122, 1660, 2086, 2266, 2328, 2906
XapI	RAATTY	5 cut(s)	1361, 1842, 2315, 2553, 2644
XceI	RCATGY	3 cut(s)	408, 899, 1981
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2729
XhoI	CTCGAG	4 cut(s)	239, 821, 1527, 2507
XmiI	GTMKAC	1 cut(s)	1751
XmnI	GAANNNNTTC	3 cut(s)	1099, 1152, 2708
XspI	CTAG	4 cut(s)	203, 399, 645, 2540
ZrmI	AGTACT	1 cut(s)	272
Zsp2I	ATGCAT	1 cut(s)	2971

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.