Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0010393

Physical Location & Seq

Genomic Coordinates Forward (+)

65656543 .. 65664253

Gene Structure 7711 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00386930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3039 bp

ATGAGTGGATTCCTTGGGAAAGGAGTGATAGCGTTCAACTCTTCTCTTCTATTCAGACGTTACCCTTTCTCTTTCGCGCCGTCACACCTCCCTCGGAATCGGAATCGGAATCGCTCCCTCACCACCGCCAAGCACTTGGCTTCAATTGCAACGTCGTCGTTTCGTCCCAAGATGCCCGGCGTCGAATCTCAGGGAGGAGGACTAGAGTGGCCGGCACAGCGCGTGCGGGACACCTTCCTCGAGTTCTTCAGGGATGAGAAGAAACACGAGTACTGGAAGTCAAGCCCGGTCGTCCCCCACAACGATCCAACTCTTCTCTTTGCTAATGCTGGAATGAACCAGTTTAAGCCCATTTTTTTGGGGACGGCGGACCCGAACACTGGGCTAAGCAAGCTGACTAGAGCATGCAACACGCAGAAATGTATAAGGGCAGGAGGGAAGCATAATGATCTCGAAGATGTAGGGAAAGACACCTACCATCACACTTTTTTCGAGATGCTTGGTAATTGGTCGTTCGGGGACTATTTCAAGAGTGAAGCCATTGAGTGGGCTTGGAAGCTTCTCACTCAGGTTTATAAGCTGCCAGAAGATCGATTTTATGCCACTTATTTTGGCGGTGATGAGAAGCTGGGTCTTGCTCCTGATTATGAAGCTAGAGATTTATGGCTTAAGTTTCTACCACCTGGACGTGTACTGCCTTTTGGCTGTAAGGATAACTTTTGGGAGATGGGTGATACTGGTCCTTGCGGACCTTGCACTGAGATCCATTATGATAGAGTTGGTAACCGGGATGCTGCATCATTAGTCAACTATGATGACCCTACCTGCCTCGAGATCTGGAACCTTGTCTTTATTCAGTTCAATAGGGAAAGTGATGGCTCTCTTAAACCATTGCCTGCTAAGCATGTTGACACTGGGATGGGCTTTGAAAGATTGACTTCTGTACTTCAGAACAAGATGAGCAATTATGACACTGATGTCTTCATGCCTATATTTGATGCTATTCAGCAGGCTACAGGGGCTCGCCCATATTCTGGGAAAGTTGGAGATGATGATACAGACAAAGTTGACATGGCATACAGGGTTGTTGCAGATCATATAAGAACCCTTTCATTTGCCATTGCTGATGGGTCCCGTCCAGGCAATGATGGTCGTGAATATGTTCTGAGACGTATTCTCCGTCGAGCTGTCCGATATGGAAGAGAAGTGCTAAAAGCTCAAGAAGGATTTTTCAATGGGCTTGTACCGGTGTTGGCGGCATTGATGGGTGATGTATTTCCGGAGCTACAACAACATGAAGCAGGCATTAGGGAGATAATCAAAGAGGAGGAAGAAGCATTTGGCAAGACTCTAATTAAGGGAATAGAAAAATTCAAAAAGGCAGCTCAAGATGTTCAAGGCAAAACATTTAGTGGGCAGGAGGCTTTTGTCTTGTGGGACACATTTGGGTTCCCGTTAGATTTGACTCAGTTGATGGCAGAGGAACAGGGATTAGAAGTTGATGTTGAGGGTTTCAATGTCGCTATGGATGAGGCTCGAGAAAGATCAAGGAATGCTCAAAACAAGGAAGAAGGTGGTGCCATTGTTATGAATGCTGAAGCTACTGCTTCATTGCACCAGAGTGGAATTGCTGCAACAGACGACAGCTTCAAATATATTTGGTTTCAGGACCATGAAAGTGTAATTAAAGCAATTTACACTGGTTCAGAGTTTATGGATAATGTTGCTGCTGGCAGCTTAGTTGGTGTTATTTTGGAGTCTACAAGTTTCTATGCAGAGCAAGGTGGACAGATATTTGATACTGGATCACTAAAAGGAGAGACTGGCTCATTTCAAGTCCGCAATGTTCAAATTTTTGGAGGTTTTGTTGTCCACATTGGTTCCTTCTCTGGAGAGCATGGCAAACTCTCTGTGGGTGACAAAGTGGTTTGTAAGGTTGACTATAAAAGGCGTACACTCATTGCGCCTAACCATACTTGCACACACATGTTGAACTTTGCTCTGAGGGAAGTTCTTGGCAGTCATGTTGACCAGAAGGGTTCCATTGTTCTTCCTGAAAAATTGAGATTTGATTTTTCTCATGGTAAGCCGGTGGACCCTGAACAGTTGAGAAGAATTGAATCAATTGTGAATGAGCAAATAAAAGCTGAATTAGATGTTTCTGTTAAGGAGGTAGCCCTGGTTGAAGCTGAGCGCATCAATGGTTTAAGGGCTGTATTTGGAGAGGTCTATCCTGACCCAGTAAGAGTTGTGGCTGTTGGGCGAAAAGTTGAGGACCTCCTGTCTGACCCTAAAAATGAGGAATGGTCATCAATATCAACAGAATTTTGTGGAGGGACCCATATAACAAACACACGTGAAGCTGAGGCCTTTGCCCTTTTATCTGAAGAGGGAACTGCTAAGGGAATCCGAAGGATAACTGCTGTCACAACTAATGCTGCTTTTGATGCAATTAAATTGGCAGATTCACTTGAGCAGGAAGTAATTGAAGCTTCCAAGGCTGAAGAGAGCTTGCTCGAGAAGAAGATAGCTTCCTTTAAATCTCGCCTAGACGCAGCACAAATTCCAGCAGCTAAGAAAGCTGATATCAGGGACAAGATTGCCCAACTTCAGAACAAAGTGAGAAAGGCACAGAAGAAGATTGCAGAGCAAAATTTACAGAAAGCTGTCAAGGTTGCAAGGGAGATGGCGGAAGTTGCTGCATCAGATGGGAAGTCTTTCTGTGTATCCCATGTTGATGTTGGTTTGGATACTGCTGCAGTTCGTGAAGCAGTTTGTAAAGTCATAGAGAAGGGAATGCCTGCGATGGTTTTTAGCACGGATGAAACAACAAATAAGGCTGTGGTGTGTGCTGGAGTACCAGGAGACAAAGGCAAGCAATTGGAGGTGTCAGAGTGGTTGACTGCAGCTATGGGTCCCCTGAAAGGAAGGGGTGGTAAAGGAAAAGGTGGCCTTGCTACTGGCCAGGGAACTGATGCATCTCATCTAAACGAGGCAATAGACCTGGCAACTACTTTTGCGCAGATGAAATTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1012

Amino Acids

111.74

Weight (kDa)

5.87

Isoelectric Point (pI)

34.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	75 - 650	8.9e-219	tRNA synthetases class II (A)
tRNA_SAD	PF07973	748 - 806	4.9e-12	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	905 - 1007	9.4e-10	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50200 AT1G50200
fragaria_vesca	FvH4_4g36780 FvH4_4g36780
malus_domestica	MD13G1007000.v1.1 MD16G1000800.v1.1
prunus_persica	Prupe.1G345900_v2.0.a1
pyrus_communis	pycom13g00590 pycom16g00460
rosa_chinensis	RchiOBHm_Chr4g0446511
rosa_laevigata	RLG00000005653
rosa_multiflora	Rmu_co8401425.1_g000001 Rmu_sc0005526.1_g000002 Rmu_sc0009884.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386930
rosa_rugosa	Rorug04G0372100 Rorug04G0372200
rosa_samantha	Rh4BG442800 Rh4CG460300 Rh4DG441500
rosa_wichuraiana	Rw4G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	576
AasI	GACNNNNNNGTC	1 cut(s)	977
Acc16I	TGCGCA	1 cut(s)	3021
Acc36I	ACCTGC	1 cut(s)	833
AccB1I	GGYRCC	1 cut(s)	1577
AccB7I	CCANNNNNTGG	2 cut(s)	546, 1034
AccI	GTMKAC	1 cut(s)	1760
AccII	CGCG	2 cut(s)	77, 222
AccIII	TCCGGA	1 cut(s)	1279
AciI	CCGC	8 cut(s)	126, 226, 368, 615, 747, 1256, 1840, 2690
AclWI	GGATC	3 cut(s)	299, 757, 1813
AcoI	YGGCCR	2 cut(s)	209, 2962
AcsI	RAATTY	5 cut(s)	1370, 1851, 2324, 2562, 2653
AcuI	CTGAAG	6 cut(s)	232, 932, 1617, 2406, 2523, 2594
AcvI	CACGTG	1 cut(s)	2357
AcyI	GRCGYC	1 cut(s)	180
AfaI	GTAC	6 cut(s)	272, 693, 945, 1245, 1954, 2859
AfiI	CCNNNNNNNGG	4 cut(s)	380, 546, 1034, 2924
AflII	CTTAAG	1 cut(s)	668
AflIII	ACRYGT	3 cut(s)	688, 1986, 2354
AgeI	ACCGGT	1 cut(s)	1246
AhdI	GACNNNNNGTC	1 cut(s)	2281
AjiI	CACGTC	1 cut(s)	689
AjnI	CCWGG	6 cut(s)	682, 1138, 2178, 2860, 2964, 3003
AjuI	GAANNNNNNNTTGG	2 cut(s)	1323, 1355
AleI	CACNNNNGTG	1 cut(s)	1620
Alw26I	GTCTC	3 cut(s)	1162, 1814, 2859
AlwI	GGATC	3 cut(s)	299, 757, 1813
Ama87I	CYCGRG	4 cut(s)	239, 830, 1536, 2516
Aor13HI	TCCGGA	1 cut(s)	1279
AoxI	GGCC	4 cut(s)	209, 2367, 2950, 2962
ApoI	RAATTY	5 cut(s)	1370, 1851, 2324, 2562, 2653
AsiGI	ACCGGT	1 cut(s)	1246
Asp700I	GAANNNNTTC	3 cut(s)	1108, 1161, 2717
AspLEI	GCGC	5 cut(s)	79, 222, 1966, 2196, 3022
AspS9I	GGNCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
AsuC2I	CCSGG	3 cut(s)	177, 287, 788
AsuHPI	GGTGA	5 cut(s)	112, 629, 743, 1280, 1928
AvaI	CYCGRG	4 cut(s)	239, 830, 1536, 2516
AvaII	GGWCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
BalI	TGGCCA	1 cut(s)	2964
BanI	GGYRCC	1 cut(s)	1577
BanII	GRGCYC	1 cut(s)	1024
BauI	CACGAG	1 cut(s)	266
BbrPI	CACGTG	1 cut(s)	2357
BbsI	GAAGAC	1 cut(s)	973
BbvCI	CCTCAGC	1 cut(s)	2364
BceAI	ACGGC	2 cut(s)	64, 381
BcgI	CGANNNNNNTGC	2 cut(s)	138, 172
BciT130I	CCWGG	6 cut(s)	684, 1140, 2180, 2862, 2966, 3005
BciVI	GTATCC	2 cut(s)	2737, 2743
BcnI	CCSGG	3 cut(s)	177, 287, 788
BcoDI	GTCTC	3 cut(s)	1162, 1814, 2859
BfaI	CTAG	4 cut(s)	203, 399, 654, 2549
BfmI	CTRYAG	3 cut(s)	1014, 2757, 2904
BfrI	CTTAAG	1 cut(s)	668
BfuAI	ACCTGC	1 cut(s)	833
BfuI	GTATCC	2 cut(s)	2737, 2743
BglII	AGATCT	1 cut(s)	834
BlpI	GCTNAGC	3 cut(s)	386, 900, 2190
BmcAI	AGTACT	1 cut(s)	272
Bme1390I	CCNGG	9 cut(s)	177, 287, 684, 788, 1140, 2180, 2862, 2966, 3005
Bme18I	GGWCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
BmeRI	GACNNNNNGTC	1 cut(s)	2281
BmeT110I	CYCGRG	4 cut(s)	239, 830, 1536, 2516
BmgBI	CACGTC	1 cut(s)	689
BmgT120I	GGNCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
BmrFI	CCNGG	9 cut(s)	177, 287, 684, 788, 1140, 2180, 2862, 2966, 3005
BmrI	ACTGGG	3 cut(s)	390, 924, 2234
BmuI	ACTGGG	3 cut(s)	390, 924, 2234
BpiI	GAAGAC	1 cut(s)	973
BplI	GAGNNNNNCTC	2 cut(s)	1811, 1843
BpmI	CTGGAG	2 cut(s)	1911, 2874
Bpu10I	CCTNAGC	2 cut(s)	2364, 2400
Bpu1102I	GCTNAGC	3 cut(s)	386, 900, 2190
BpuEI	CTTGAG	3 cut(s)	1203, 1371, 2492
BpuMI	CCSGG	3 cut(s)	177, 287, 788
Bsa29I	ATCGAT	1 cut(s)	592
BsaAI	YACGTR	1 cut(s)	2357
BsaHI	GRCGYC	1 cut(s)	180
BsaJI	CCNNGG	5 cut(s)	13, 92, 2178, 2496, 2965
BsaWI	WCCGGW	2 cut(s)	1246, 1279
BsaXI	ACNNNNNCTCC	4 cut(s)	1161, 1191, 1851, 1881
Bsc4I	CCNNNNNNNGG	4 cut(s)	380, 546, 1034, 2924
Bse118I	RCCGGY	3 cut(s)	211, 1246, 2089
Bse3DI	GCAATG	6 cut(s)	890, 1119, 1150, 1610, 1849, 1959
BseAI	TCCGGA	1 cut(s)	1279
BseBI	CCWGG	6 cut(s)	684, 1140, 2180, 2862, 2966, 3005
BseCI	ATCGAT	1 cut(s)	592
BseDI	CCNNGG	5 cut(s)	13, 92, 2178, 2496, 2965
BseGI	GGATG	5 cut(s)	259, 796, 924, 1534, 2827
BseLI	CCNNNNNNNGG	4 cut(s)	380, 546, 1034, 2924
BseMI	GCAATG	6 cut(s)	890, 1119, 1150, 1610, 1849, 1959
BseMII	CTCAG	8 cut(s)	203, 581, 750, 1157, 1481, 1994, 2181, 2355
BseRI	GAGGAG	2 cut(s)	210, 1340
BseYI	CCCAGC	1 cut(s)	628
Bsh1236I	CGCG	2 cut(s)	77, 222
Bsh1285I	CGRYCG	1 cut(s)	291
BshFI	GGCC	4 cut(s)	211, 2369, 2952, 2964
BshNI	GGYRCC	1 cut(s)	1577
BshTI	ACCGGT	1 cut(s)	1246
BshVI	ATCGAT	1 cut(s)	592
BsiEI	CGRYCG	1 cut(s)	291
BsiHKCI	CYCGRG	4 cut(s)	239, 830, 1536, 2516
BsiSI	CCGG	7 cut(s)	177, 212, 287, 787, 1247, 1280, 2090
BslI	CCNNNNNNNGG	4 cut(s)	380, 546, 1034, 2924
BsmAI	GTCTC	3 cut(s)	1162, 1814, 2859
BsmBI	CGTCTC	1 cut(s)	1162
BsmI	GAATGC	3 cut(s)	1558, 1597, 2802
BsnI	GGCC	4 cut(s)	211, 2369, 2952, 2964
BsoBI	CYCGRG	4 cut(s)	239, 830, 1536, 2516
Bsp1286I	GDGCHC	1 cut(s)	1024
Bsp13I	TCCGGA	1 cut(s)	1279
Bsp143I	GATC	8 cut(s)	304, 448, 589, 762, 834, 1093, 1544, 1805
Bsp1720I	GCTNAGC	3 cut(s)	386, 900, 2190
BspACI	CCGC	8 cut(s)	126, 226, 368, 615, 747, 1256, 1840, 2690
BspANI	GGCC	4 cut(s)	211, 2369, 2952, 2964
BspCNI	CTCAG	8 cut(s)	202, 580, 751, 1158, 1480, 1995, 2182, 2356
BspDI	ATCGAT	1 cut(s)	592
BspEI	TCCGGA	1 cut(s)	1279
BspFNI	CGCG	2 cut(s)	77, 222
BspMAI	CTGCAG	2 cut(s)	2761, 2908
BspMI	ACCTGC	1 cut(s)	833
BspPI	GGATC	3 cut(s)	299, 757, 1813
BspT107I	GGYRCC	1 cut(s)	1577
BspTI	CTTAAG	1 cut(s)	668
BsrDI	GCAATG	6 cut(s)	890, 1119, 1150, 1610, 1849, 1959
BsrFI	RCCGGY	3 cut(s)	211, 1246, 2089
BssAI	RCCGGY	3 cut(s)	211, 1246, 2089
BssECI	CCNNGG	5 cut(s)	13, 92, 2178, 2496, 2965
BssMI	GATC	8 cut(s)	304, 448, 589, 762, 834, 1093, 1544, 1805
BssNI	GRCGYC	1 cut(s)	180
BssSI	CACGAG	1 cut(s)	266
BssT1I	CCWWGG	2 cut(s)	13, 2496
Bst2BI	CACGAG	1 cut(s)	266
Bst2UI	CCWGG	6 cut(s)	684, 1140, 2180, 2862, 2966, 3005
Bst4CI	ACNGT	1 cut(s)	2106
Bst6I	CTCTTC	6 cut(s)	46, 51, 318, 1195, 2382, 2499
BstACI	GRCGYC	1 cut(s)	180
BstAFI	CTTAAG	1 cut(s)	668
BstBAI	YACGTR	1 cut(s)	2357
BstEII	GGTNACC	1 cut(s)	782
BstF5I	GGATG	5 cut(s)	259, 796, 924, 1534, 2827
BstFNI	CGCG	2 cut(s)	77, 222
BstHHI	GCGC	5 cut(s)	79, 222, 1966, 2196, 3022
BstKTI	GATC	8 cut(s)	307, 451, 592, 765, 837, 1096, 1547, 1808
BstMAI	GTCTC	3 cut(s)	1162, 1814, 2859
BstMBI	GATC	8 cut(s)	304, 448, 589, 762, 834, 1093, 1544, 1805
BstMCI	CGRYCG	1 cut(s)	291
BstNI	CCWGG	6 cut(s)	684, 1140, 2180, 2862, 2966, 3005
BstNSI	RCATGY	3 cut(s)	408, 908, 1990
BstPI	GGTNACC	1 cut(s)	782
BstSCI	CCNGG	9 cut(s)	175, 285, 682, 786, 1138, 2178, 2860, 2964, 3003
BstSFI	CTRYAG	3 cut(s)	1014, 2757, 2904
BstUI	CGCG	2 cut(s)	77, 222
BstV2I	GAAGAC	1 cut(s)	973
BstX2I	RGATCY	2 cut(s)	762, 834
BstXI	CCANNNNNNTGG	2 cut(s)	136, 358
BstYI	RGATCY	2 cut(s)	762, 834
Bsu15I	ATCGAT	1 cut(s)	592
BsuI	GTATCC	2 cut(s)	2737, 2743
BsuRI	GGCC	4 cut(s)	211, 2369, 2952, 2964
BsuTUI	ATCGAT	1 cut(s)	592
BtgZI	GCGATG	1 cut(s)	2819
BtrI	CACGTC	1 cut(s)	689
BtsCI	GGATG	5 cut(s)	259, 796, 924, 1534, 2827
BtsIMutI	CAGTG	5 cut(s)	378, 756, 912, 972, 1698
BveI	ACCTGC	1 cut(s)	833
CfoI	GCGC	5 cut(s)	79, 222, 1966, 2196, 3022
Cfr10I	RCCGGY	3 cut(s)	211, 1246, 2089
Cfr13I	GGNCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
ClaI	ATCGAT	1 cut(s)	592
CseI	GACGC	2 cut(s)	169, 2561
Csp6I	GTAC	6 cut(s)	271, 692, 944, 1244, 1953, 2858
CspAI	ACCGGT	1 cut(s)	1246
CspCI	CAANNNNNGTGG	2 cut(s)	592, 627
CviQI	GTAC	6 cut(s)	271, 692, 944, 1244, 1953, 2858
DpnI	GATC	8 cut(s)	306, 450, 591, 764, 836, 1095, 1546, 1807
DpnII	GATC	8 cut(s)	304, 448, 589, 762, 834, 1093, 1544, 1805
DraI	TTTAAA	1 cut(s)	2539
DrdI	GACNNNNNNGTC	1 cut(s)	977
DriI	GACNNNNNGTC	1 cut(s)	2281
DseDI	GACNNNNNNGTC	1 cut(s)	977
EaeI	YGGCCR	2 cut(s)	209, 2962
Eam1104I	CTCTTC	6 cut(s)	46, 51, 318, 1195, 2382, 2499
Eam1105I	GACNNNNNGTC	1 cut(s)	2281
EarI	CTCTTC	6 cut(s)	46, 51, 318, 1195, 2382, 2499
EciI	GGCGGA	2 cut(s)	383, 2705
Eco130I	CCWWGG	2 cut(s)	13, 2496
Eco147I	AGGCCT	1 cut(s)	2369
Eco24I	GRGCYC	1 cut(s)	1024
Eco32I	GATATC	1 cut(s)	2587
Eco47I	GGWCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
Eco57I	CTGAAG	6 cut(s)	232, 932, 1617, 2406, 2523, 2594
Eco72I	CACGTG	1 cut(s)	2357
Eco88I	CYCGRG	4 cut(s)	239, 830, 1536, 2516
Eco91I	GGTNACC	1 cut(s)	782
EcoO109I	RGGNCCY	4 cut(s)	1131, 2275, 2337, 2915
EcoO65I	GGTNACC	1 cut(s)	782
EcoRII	CCWGG	6 cut(s)	682, 1138, 2178, 2860, 2964, 3003
EcoRV	GATATC	1 cut(s)	2587
EcoT14I	CCWWGG	2 cut(s)	13, 2496
EcoT22I	ATGCAT	1 cut(s)	2980
EcoT38I	GRGCYC	1 cut(s)	1024
ErhI	CCWWGG	2 cut(s)	13, 2496
Esp3I	CGTCTC	1 cut(s)	1162
FalI	AAGNNNNNCTT	6 cut(s)	701, 733, 922, 954, 2937, 2969
FauI	CCCGC	1 cut(s)	219
FblI	GTMKAC	1 cut(s)	1760
FokI	GGATG	5 cut(s)	266, 803, 931, 1541, 2834
FriOI	GRGCYC	1 cut(s)	1024
FspBI	CTAG	4 cut(s)	203, 399, 654, 2549
FspI	TGCGCA	1 cut(s)	3021
GlaI	GCGC	5 cut(s)	78, 221, 1965, 2195, 3021
GsaI	CCCAGC	1 cut(s)	632
GsuI	CTGGAG	2 cut(s)	1911, 2874
HaeIII	GGCC	4 cut(s)	211, 2369, 2952, 2964
HapII	CCGG	7 cut(s)	177, 212, 287, 787, 1247, 1280, 2090
HgaI	GACGC	2 cut(s)	169, 2561
HhaI	GCGC	5 cut(s)	79, 222, 1966, 2196, 3022
Hin1I	GRCGYC	1 cut(s)	180
Hin6I	GCGC	5 cut(s)	77, 220, 1964, 2194, 3020
HinP1I	GCGC	5 cut(s)	77, 220, 1964, 2194, 3020
HincII	GTYRAC	6 cut(s)	808, 910, 1069, 1939, 2029, 2901
HindII	GTYRAC	6 cut(s)	808, 910, 1069, 1939, 2029, 2901
HindIII	AAGCTT	2 cut(s)	557, 2490
HpaII	CCGG	7 cut(s)	177, 212, 287, 787, 1247, 1280, 2090
HphI	GGTGA	5 cut(s)	112, 629, 743, 1280, 1928
Hpy99I	CGWCG	4 cut(s)	157, 160, 185, 1185
HpyAV	CCTTC	8 cut(s)	244, 1217, 1565, 1894, 2029, 2406, 2785, 2922
HpyCH4III	ACNGT	1 cut(s)	2106
HpyCH4IV	ACGT	5 cut(s)	58, 152, 688, 1171, 2356
HpySE526I	ACGT	5 cut(s)	58, 152, 688, 1171, 2356
Hsp92I	GRCGYC	1 cut(s)	180
HspAI	GCGC	5 cut(s)	77, 220, 1964, 2194, 3020
KflI	GGGWCCC	3 cut(s)	1131, 2337, 2915
Kpn2I	TCCGGA	1 cut(s)	1279
KroI	GCCGGC	1 cut(s)	211
KroNI	GCCGGC	1 cut(s)	213
Kzo9I	GATC	8 cut(s)	304, 448, 589, 762, 834, 1093, 1544, 1805
LmnI	GCTCC	3 cut(s)	119, 643, 1282
MaeI	CTAG	4 cut(s)	203, 399, 654, 2549
MaeII	ACGT	5 cut(s)	58, 152, 688, 1171, 2356
MaeIII	GTNAC	5 cut(s)	59, 81, 782, 1916, 2425
MalI	GATC	8 cut(s)	306, 450, 591, 764, 836, 1095, 1546, 1807
MboI	GATC	8 cut(s)	304, 448, 589, 762, 834, 1093, 1544, 1805
MfeI	CAATTG	3 cut(s)	144, 2124, 2879
MflI	RGATCY	2 cut(s)	762, 834
MhlI	GDGCHC	1 cut(s)	1024
MlsI	TGGCCA	1 cut(s)	2964
MluNI	TGGCCA	1 cut(s)	2964
MlyI	GAGTC	3 cut(s)	1342, 1459, 1766
MmeI	TCCRAC	2 cut(s)	332, 1024
Mox20I	TGGCCA	1 cut(s)	2964
Mph1103I	ATGCAT	1 cut(s)	2980
MroI	TCCGGA	1 cut(s)	1279
MroNI	GCCGGC	1 cut(s)	211
MroXI	GAANNNNTTC	3 cut(s)	1108, 1161, 2717
MscI	TGGCCA	1 cut(s)	2964
MseI	TTAA	9 cut(s)	345, 669, 885, 1356, 1686, 2166, 2207, 2454, 2538
MslI	CAYNNNNRTG	6 cut(s)	917, 1586, 1620, 1677, 1985, 2736
Msp20I	TGGCCA	1 cut(s)	2964
MspCI	CTTAAG	1 cut(s)	668
MspI	CCGG	7 cut(s)	177, 212, 287, 787, 1247, 1280, 2090
MspR9I	CCNGG	9 cut(s)	177, 287, 684, 788, 1140, 2180, 2862, 2966, 3005
MunI	CAATTG	3 cut(s)	144, 2124, 2879
Mva1269I	GAATGC	3 cut(s)	1558, 1597, 2802
MvaI	CCWGG	6 cut(s)	684, 1140, 2180, 2862, 2966, 3005
MvnI	CGCG	2 cut(s)	77, 222
NaeI	GCCGGC	1 cut(s)	213
NciI	CCSGG	3 cut(s)	177, 287, 788
NdeII	GATC	8 cut(s)	304, 448, 589, 762, 834, 1093, 1544, 1805
NgoMIV	GCCGGC	1 cut(s)	211
NmuCI	GTSAC	3 cut(s)	81, 1916, 2425
NsbI	TGCGCA	1 cut(s)	3021
NsiI	ATGCAT	1 cut(s)	2980
NspI	RCATGY	3 cut(s)	408, 908, 1990
OliI	CACNNNNGTG	1 cut(s)	1620
PaeI	GCATGC	1 cut(s)	408
PaeR7I	CTCGAG	4 cut(s)	239, 830, 1536, 2516
PceI	AGGCCT	1 cut(s)	2369
PciI	ACATGT	1 cut(s)	1986
PcsI	WCGNNNNNNNCGW	3 cut(s)	371, 1177, 1189
PctI	GAATGC	3 cut(s)	1558, 1597, 2802
PdiI	GCCGGC	1 cut(s)	213
PdmI	GAANNNNTTC	3 cut(s)	1108, 1161, 2717
PfeI	GAWTC	8 cut(s)	9, 97, 103, 109, 185, 2120, 2406, 2465
PflMI	CCANNNNNTGG	2 cut(s)	546, 1034
PinAI	ACCGGT	1 cut(s)	1246
PleI	GAGTC	3 cut(s)	1342, 1459, 1765
PmaCI	CACGTG	1 cut(s)	2357
PmlI	CACGTG	1 cut(s)	2357
PpsI	GAGTC	3 cut(s)	1342, 1459, 1765
Ppu21I	YACGTR	1 cut(s)	2357
PpuMI	RGGWCCY	4 cut(s)	1131, 2275, 2337, 2915
PscI	ACATGT	1 cut(s)	1986
PsiI	TTATAA	1 cut(s)	576
Psp5II	RGGWCCY	4 cut(s)	1131, 2275, 2337, 2915
Psp6I	CCWGG	6 cut(s)	682, 1138, 2178, 2860, 2964, 3003
PspCI	CACGTG	1 cut(s)	2357
PspEI	GGTNACC	1 cut(s)	782
PspFI	CCCAGC	1 cut(s)	628
PspGI	CCWGG	6 cut(s)	682, 1138, 2178, 2860, 2964, 3003
PspPI	GGNCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
PspPPI	RGGWCCY	4 cut(s)	1131, 2275, 2337, 2915
PspXI	VCTCGAGB	1 cut(s)	239
PstI	CTGCAG	2 cut(s)	2761, 2908
PsuI	RGATCY	2 cut(s)	762, 834
RsaI	GTAC	6 cut(s)	272, 693, 945, 1245, 1954, 2859
RsaNI	GTAC	6 cut(s)	271, 692, 944, 1244, 1953, 2858
RseI	CAYNNNNRTG	6 cut(s)	917, 1586, 1620, 1677, 1985, 2736
SaqAI	TTAA	9 cut(s)	345, 669, 885, 1356, 1686, 2166, 2207, 2454, 2538
Sau3AI	GATC	8 cut(s)	304, 448, 589, 762, 834, 1093, 1544, 1805
Sau96I	GGNCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
ScaI	AGTACT	1 cut(s)	272
SchI	GAGTC	3 cut(s)	1342, 1459, 1766
ScrFI	CCNGG	9 cut(s)	177, 287, 684, 788, 1140, 2180, 2862, 2966, 3005
SduI	GDGCHC	1 cut(s)	1024
SfcI	CTRYAG	3 cut(s)	1014, 2757, 2904
Sfr274I	CTCGAG	4 cut(s)	239, 830, 1536, 2516
SinI	GGWCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
SlaI	CTCGAG	4 cut(s)	239, 830, 1536, 2516
SmiMI	CAYNNNNRTG	6 cut(s)	917, 1586, 1620, 1677, 1985, 2736
SmlI	CTYRAG	8 cut(s)	239, 668, 830, 1218, 1386, 1536, 2471, 2516
SmoI	CTYRAG	8 cut(s)	239, 668, 830, 1218, 1386, 1536, 2471, 2516
SphI	GCATGC	1 cut(s)	408
SseBI	AGGCCT	1 cut(s)	2369
SsiI	CCGC	8 cut(s)	126, 226, 368, 615, 747, 1256, 1840, 2690
SspMI	CTAG	4 cut(s)	203, 399, 654, 2549
StuI	AGGCCT	1 cut(s)	2369
StyD4I	CCNGG	9 cut(s)	175, 285, 682, 786, 1138, 2178, 2860, 2964, 3003
StyI	CCWWGG	2 cut(s)	13, 2496
TaaI	ACNGT	1 cut(s)	2106
TaiI	ACGT	5 cut(s)	61, 155, 691, 1174, 2359
TaqI	TCGA	9 cut(s)	183, 240, 453, 492, 592, 831, 1183, 1537, 2517
TatI	WGTACW	3 cut(s)	270, 691, 943
TauI	GCSGC	1 cut(s)	1259
TfiI	GAWTC	8 cut(s)	9, 97, 103, 109, 185, 2120, 2406, 2465
Tru1I	TTAA	9 cut(s)	345, 669, 885, 1356, 1686, 2166, 2207, 2454, 2538
Tru9I	TTAA	9 cut(s)	345, 669, 885, 1356, 1686, 2166, 2207, 2454, 2538
TscAI	CASTG	5 cut(s)	385, 763, 919, 979, 1705
TseFI	GTSAC	3 cut(s)	81, 1916, 2425
Tsp45I	GTSAC	3 cut(s)	81, 1916, 2425
TspDTI	ATGAA	9 cut(s)	350, 663, 973, 1101, 1311, 1599, 1604, 1689, 2838
TspGWI	ACGGA	2 cut(s)	1169, 2834
TspRI	CASTG	5 cut(s)	385, 763, 919, 979, 1705
Van91I	CCANNNNNTGG	2 cut(s)	546, 1034
Vha464I	CTTAAG	1 cut(s)	668
VpaK11BI	GGWCC	9 cut(s)	370, 740, 749, 1131, 1669, 2095, 2275, 2337, 2915
XapI	RAATTY	5 cut(s)	1370, 1851, 2324, 2562, 2653
XceI	RCATGY	3 cut(s)	408, 908, 1990
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2738
XhoI	CTCGAG	4 cut(s)	239, 830, 1536, 2516
XmiI	GTMKAC	1 cut(s)	1760
XmnI	GAANNNNTTC	3 cut(s)	1108, 1161, 2717
XspI	CTAG	4 cut(s)	203, 399, 654, 2549
ZrmI	AGTACT	1 cut(s)	272
Zsp2I	ATGCAT	1 cut(s)	2980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.