Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0010393

Physical Location & Seq

Genomic Coordinates Forward (+)

63785036 .. 63792553

Gene Structure 7518 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G037060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3021 bp

ATGAGTGGATTCCTTGCGAAAGGAGTGATAGCGTTCAACCCTCTTCTTCTACTCGGACGTTACCCTTTCTCTTTCGCGCCGTCACACCTCCCTCGGAATCGGAATCGGAATCGGAATCGCTCCCTCACCACCGCCAAGCACTTGGCTTCAATCGCAACGTCGTCGTTTCGTCCCAAGATGCCCGGCGTCGAATCTCAGGGAGGAGGACTAGAGTGGCCGGCACAGCGCGTGCGGGACACCTTCCTCGAGTTCTTCAGGGATGAGAAGAAACACGAGTACTGGAAGTCAAGCCCGGTCGTCCCCCACAACGACCCAACTCTTCTCTTTGCTAATGCTGGAATGAACCAGTTTAAGCCCATTTTTTTGGGGACGGCGGACCCGAATACTGGGCTAAGCAAGCTGACTAGAGCATGCAACACGCAGAAATGTATAAGGGCAGGAGGGAAGCATAATGATCTGGAAGATGTAGGGAAAGACACCTACCATCACACTTTTTTCGAGATGCTTGGTAATTGGTCGTTCGGGGACTATTTCAAGAGTGAAGCCATTGAATGGGCTTGGAAGCTTCTCACTCAGGTATATAAGCTGCCAGAAGATCGATTTTATGCCACTTATTTTGGTGGTGATGAGAAGCTGGGTCTTGCTCCTGATTATGAAGCTAGAGATTTATGGCTTAAGTTTCTACAACCTGGACGTGTACTGCCTTTTGGCTGTAAGGATAACTTTTGGGAGATGGGTGATACTGGTCCTTGCGGCCCTTGCACTGAGATCCATTATGATAGAGTTGGTAACCGGGATGCTGCATCATTAGTCAACTACGATGACCCTACCTGCCTCGAGATCTGGAACCTTGTCTTTATTCAGTTCAATAGGGAAAGTGATGGCTCTCTTAAACCATTGCCTGCTAAGCATGTTGACACTGGGATGGGCTTTGAAAGATTGACTTCTGTACTTCAGAACAAGATGAGCAATTATGACACTGATGTCTTCATGCCTATATTTGATGCTATTCAGCAGGCTACAGGGGCTCGCCCATATTCTGGGAAAGTTGGAGATGATGATACAGACAAAGTTGACATGGCTTACAGGGTTGTTGCAGATCATATAAGAACTCTTTCATTTGCCATTGCTGATGGGTCCCGTCCAGGCAATGATGGTCGTGAATATGTTCTGAGACGTATTCTCCGTCGAGCTGTCCGATATGGAAGAGAAGTGCTAAAAGCTCAAGAAGGATTTTTCAATGGGCTTGTACCGGTGTTGGCGGCACTGATGGGTGATGTATTTCCGGAGCTAAAACAACATGAAGCAGGCATTAGGGAGATAATCAAAGAGGAGGAAGAAGCATTTGGCAAGACTCTAATTAAGGGAATAGAAAAATTCAAAAAGGCAGCTGAAGATGTTCAAGGCAAAACATTTAGTGGGCAGGAGGCTTTTGTCTTGTGGGACACATTTGGGTTCCCGTTAGATTTGACTCAGTTGATGGCAGAGGAACAGGGATTAGAAGTTGATGTTGAGGGTTTCAATGTCGCTATGGATGAGGCTCGAGAAAGATCAAGGAATGCTCAAAATAAGGAAGAAGGTGGTGCCATTGTTATGAATGCTGAAGCTACTGCTTCATTGCACAAGAGTGGAATTGCTGCAACAGACGACAGCTTCAAATATATTTGGTTTCAGGACCATGAAAGTGTAATTAAAGCAATTTACACTGGTTCAGAGTTTATGGATAATGTTGCTGCTGGCAGCTTAGTTGGTGTTATTTTGGAGTCTACAAGTTTCTATGCAGAGCAAGGTGGACAGATATTTGATACTGGATCACTAAAAGGAGAGACCGGCTCATTTCAAGTCCGCAATGTTCAAATTTTTGGAGGTTTTGTTGTCCACATTGGTTCCTTCTCTGGAGAGCATGGCAAACTCTCTGTGGGTGACAAAGTGGTTTGTAAGGTTGACTATAAAAGGCGTACACTCATTGCGCCTAACCATACTTGCACACACATGTTGAACTTTGCTCTGAGGGAAGTTCTTGGCAGTCATGTTGACCAGAAGGGTTCCATTGTTCTTCCTGAAAAATTGAGATTTGATTTTTCTCATGTTGAGAAAATTGAATCAATTGTGAATGAGCAAATAAAAGCTGAATTAGATGTTTCTGTTAAGGAGGTAGCCCTGGTTGAAGCTGAGCGCATCAATGGTTTAAGGGCTGTATTTGGAGAGGTCTATCCTGACCCAGTAAGAGTAGTGGCTGTTGGGCGAAAAGTTGAGGACCTCCTGTCTGACCCTAAAAATGAGGAATGGTCATCAATATCAACAGAATTTTGTGGAGGGACCCATATAACAAACACACGTGAAGCTGAGGCCTTTGCCCTTTTATCTGAAGAGGGAACTGCTAAGGGAATCCGAAGGATAACTGCTGTCACAACTAATGCTGCTTTTGATGCAATTAAATTGGCAGATTCACTTGAGCAGGAAGTAATTGAAGCTTCCAAGGCTGAAGAGAGCTTGCTGGAGAAGAAGATAGCTTCCTTTAAATCTCGCCTAGATGCAGCACAAATTCCAGCGGCTAAGAAAGCTGATATCAGGGACAAGATTGCCCAACTTCAGAACAAAGTGAGAAAGGCACAGAAGAAGATTGCAGAGCAAAATTTACAGAAAGCTGTCAAGGTTGCAAGGGAGATGGCGGAAGTTGCTGCATCAGATGGGAAGTCTTTCTGTGTATCCCATGTTGATGTTGGTTTGGATACTGCTGCAGTTCGTGAAGCAGTTTGTAAAGTCATAGAGAAGGGAATGCCTGCGATGGTTTTTAGCACAGATGAAACAACAAATAAGGCTGTGGTGTGTGCTGGAGTACCAGGAGACAAAGGCAAGCAATTGGAGGTGTCAGAGTGGTTGACTGCAGCTATGGGTCCCCTGAAAGGAAGGGGTGGTAAAGGAAAAGGTGGCCTTGCTACTGGCCAGGGAACAGATGCATCTCATCTAAACGAGGCAATAGATCTAGCAACCACCTTTGCGCAGATGAAATTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1006

Amino Acids

111.04

Weight (kDa)

5.87

Isoelectric Point (pI)

32.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	77 - 652	1.7e-218	tRNA synthetases class II (A)
tRNA_SAD	PF07973	742 - 800	4.8e-12	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	899 - 1001	9.3e-10	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50200 AT1G50200
fragaria_vesca	FvH4_4g36780 FvH4_4g36780
malus_domestica	MD13G1007000.v1.1 MD16G1000800.v1.1
prunus_persica	Prupe.1G345900_v2.0.a1
pyrus_communis	pycom13g00590 pycom16g00460
rosa_chinensis	RchiOBHm_Chr4g0446511
rosa_laevigata	RLG00000005653
rosa_multiflora	Rmu_co8401425.1_g000001 Rmu_sc0005526.1_g000002 Rmu_sc0009884.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386930
rosa_rugosa	Rorug04G0372100 Rorug04G0372200
rosa_samantha	Rh4BG442800 Rh4CG460300 Rh4DG441500
rosa_wichuraiana	Rw4G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	983
Acc16I	TGCGCA	1 cut(s)	3003
Acc36I	ACCTGC	1 cut(s)	839
AccB1I	GGYRCC	1 cut(s)	1583
AccB7I	CCANNNNNTGG	2 cut(s)	552, 1040
AccI	GTMKAC	1 cut(s)	1766
AccII	CGCG	2 cut(s)	77, 228
AccIII	TCCGGA	1 cut(s)	1285
AciI	CCGC	8 cut(s)	132, 232, 374, 753, 1262, 1846, 2552, 2672
AclWI	GGATC	2 cut(s)	763, 1819
AcoI	YGGCCR	2 cut(s)	215, 2944
AcsI	RAATTY	5 cut(s)	1376, 1857, 2306, 2544, 2635
AcuI	CTGAAG	7 cut(s)	238, 938, 1413, 1623, 2388, 2505, 2576
AcvI	CACGTG	1 cut(s)	2339
AcyI	GRCGYC	1 cut(s)	186
AfaI	GTAC	6 cut(s)	278, 699, 951, 1251, 1960, 2841
AfiI	CCNNNNNNNGG	4 cut(s)	386, 552, 1040, 2906
AflII	CTTAAG	1 cut(s)	674
AflIII	ACRYGT	3 cut(s)	694, 1992, 2336
AgeI	ACCGGT	1 cut(s)	1252
AhdI	GACNNNNNGTC	1 cut(s)	2263
AjiI	CACGTC	1 cut(s)	695
AjnI	CCWGG	5 cut(s)	688, 1144, 2160, 2842, 2946
AjuI	GAANNNNNNNTTGG	2 cut(s)	1329, 1361
AleI	CACNNNNGTG	1 cut(s)	1626
Alw26I	GTCTC	3 cut(s)	1168, 1820, 2841
AlwI	GGATC	2 cut(s)	763, 1819
Ama87I	CYCGRG	3 cut(s)	245, 836, 1542
Aor13HI	TCCGGA	1 cut(s)	1285
AoxI	GGCC	5 cut(s)	215, 754, 2349, 2932, 2944
ApoI	RAATTY	5 cut(s)	1376, 1857, 2306, 2544, 2635
AsiGI	ACCGGT	1 cut(s)	1252
Asp700I	GAANNNNTTC	4 cut(s)	1114, 1167, 1398, 2699
AspLEI	GCGC	5 cut(s)	79, 228, 1972, 2178, 3004
AspS9I	GGNCC	8 cut(s)	376, 746, 755, 1137, 1675, 2257, 2319, 2897
AsuC2I	CCSGG	3 cut(s)	183, 293, 794
AsuHPI	GGTGA	5 cut(s)	118, 635, 749, 1286, 1934
AvaI	CYCGRG	3 cut(s)	245, 836, 1542
AvaII	GGWCC	7 cut(s)	376, 746, 1137, 1675, 2257, 2319, 2897
BalI	TGGCCA	1 cut(s)	2946
BanI	GGYRCC	1 cut(s)	1583
BanII	GRGCYC	1 cut(s)	1030
BauI	CACGAG	1 cut(s)	272
BbrPI	CACGTG	1 cut(s)	2339
BbsI	GAAGAC	1 cut(s)	979
BbvCI	CCTCAGC	1 cut(s)	2346
BceAI	ACGGC	2 cut(s)	64, 387
BcgI	CGANNNNNNTGC	2 cut(s)	144, 178
BciT130I	CCWGG	5 cut(s)	690, 1146, 2162, 2844, 2948
BciVI	GTATCC	2 cut(s)	2719, 2725
BcnI	CCSGG	3 cut(s)	183, 293, 794
BcoDI	GTCTC	3 cut(s)	1168, 1820, 2841
BfaI	CTAG	5 cut(s)	209, 405, 660, 2531, 2987
BfmI	CTRYAG	3 cut(s)	1020, 2739, 2886
BfrI	CTTAAG	1 cut(s)	674
BfuAI	ACCTGC	1 cut(s)	839
BfuI	GTATCC	2 cut(s)	2719, 2725
BglII	AGATCT	2 cut(s)	840, 2983
BlpI	GCTNAGC	3 cut(s)	392, 906, 2172
BmcAI	AGTACT	1 cut(s)	278
Bme1390I	CCNGG	8 cut(s)	183, 293, 690, 794, 1146, 2162, 2844, 2948
Bme18I	GGWCC	7 cut(s)	376, 746, 1137, 1675, 2257, 2319, 2897
BmeRI	GACNNNNNGTC	1 cut(s)	2263
BmeT110I	CYCGRG	3 cut(s)	245, 836, 1542
BmgBI	CACGTC	1 cut(s)	695
BmgT120I	GGNCC	8 cut(s)	376, 746, 755, 1137, 1675, 2257, 2319, 2897
BmrFI	CCNGG	8 cut(s)	183, 293, 690, 794, 1146, 2162, 2844, 2948
BmrI	ACTGGG	3 cut(s)	396, 930, 2216
BmuI	ACTGGG	3 cut(s)	396, 930, 2216
BpiI	GAAGAC	1 cut(s)	979
BplI	GAGNNNNNCTC	2 cut(s)	1817, 1849
BpmI	CTGGAG	3 cut(s)	1917, 2519, 2856
Bpu10I	CCTNAGC	2 cut(s)	2346, 2382
Bpu1102I	GCTNAGC	3 cut(s)	392, 906, 2172
BpuEI	CTTGAG	2 cut(s)	1209, 2474
BpuMI	CCSGG	3 cut(s)	183, 293, 794
Bsa29I	ATCGAT	1 cut(s)	598
BsaAI	YACGTR	1 cut(s)	2339
BsaHI	GRCGYC	1 cut(s)	186
BsaI	GGTCTC	1 cut(s)	1820
BsaJI	CCNNGG	4 cut(s)	92, 2160, 2478, 2947
BsaWI	WCCGGW	2 cut(s)	1252, 1285
BsaXI	ACNNNNNCTCC	4 cut(s)	1167, 1197, 1857, 1887
Bsc4I	CCNNNNNNNGG	4 cut(s)	386, 552, 1040, 2906
Bse118I	RCCGGY	3 cut(s)	217, 1252, 1829
Bse1I	ACTGG	9 cut(s)	284, 346, 391, 748, 925, 1711, 1813, 2222, 2947
Bse3DI	GCAATG	6 cut(s)	896, 1125, 1156, 1616, 1855, 1965
BseAI	TCCGGA	1 cut(s)	1285
BseBI	CCWGG	5 cut(s)	690, 1146, 2162, 2844, 2948
BseCI	ATCGAT	1 cut(s)	598
BseDI	CCNNGG	4 cut(s)	92, 2160, 2478, 2947
BseGI	GGATG	4 cut(s)	265, 802, 930, 1540
BseLI	CCNNNNNNNGG	4 cut(s)	386, 552, 1040, 2906
BseMI	GCAATG	6 cut(s)	896, 1125, 1156, 1616, 1855, 1965
BseMII	CTCAG	8 cut(s)	209, 587, 756, 1163, 1487, 2000, 2163, 2337
BseNI	ACTGG	9 cut(s)	284, 346, 391, 748, 925, 1711, 1813, 2222, 2947
BseRI	GAGGAG	2 cut(s)	216, 1346
BseYI	CCCAGC	1 cut(s)	634
Bsh1236I	CGCG	2 cut(s)	77, 228
Bsh1285I	CGRYCG	1 cut(s)	297
BshFI	GGCC	5 cut(s)	217, 756, 2351, 2934, 2946
BshNI	GGYRCC	1 cut(s)	1583
BshTI	ACCGGT	1 cut(s)	1252
BshVI	ATCGAT	1 cut(s)	598
BsiEI	CGRYCG	1 cut(s)	297
BsiHKCI	CYCGRG	3 cut(s)	245, 836, 1542
BsiSI	CCGG	7 cut(s)	183, 218, 293, 793, 1253, 1286, 1830
BslI	CCNNNNNNNGG	4 cut(s)	386, 552, 1040, 2906
BsmAI	GTCTC	3 cut(s)	1168, 1820, 2841
BsmBI	CGTCTC	1 cut(s)	1168
BsmI	GAATGC	3 cut(s)	1564, 1603, 2784
BsnI	GGCC	5 cut(s)	217, 756, 2351, 2934, 2946
Bso31I	GGTCTC	1 cut(s)	1820
BsoBI	CYCGRG	3 cut(s)	245, 836, 1542
Bsp1286I	GDGCHC	1 cut(s)	1030
Bsp13I	TCCGGA	1 cut(s)	1285
Bsp143I	GATC	8 cut(s)	454, 595, 768, 840, 1099, 1550, 1811, 2983
Bsp1720I	GCTNAGC	3 cut(s)	392, 906, 2172
BspACI	CCGC	8 cut(s)	132, 232, 374, 753, 1262, 1846, 2552, 2672
BspANI	GGCC	5 cut(s)	217, 756, 2351, 2934, 2946
BspCNI	CTCAG	8 cut(s)	208, 586, 757, 1164, 1486, 2001, 2164, 2338
BspDI	ATCGAT	1 cut(s)	598
BspEI	TCCGGA	1 cut(s)	1285
BspFNI	CGCG	2 cut(s)	77, 228
BspMAI	CTGCAG	2 cut(s)	2743, 2890
BspMI	ACCTGC	1 cut(s)	839
BspPI	GGATC	2 cut(s)	763, 1819
BspT107I	GGYRCC	1 cut(s)	1583
BspTI	CTTAAG	1 cut(s)	674
BspTNI	GGTCTC	1 cut(s)	1820
BsrDI	GCAATG	6 cut(s)	896, 1125, 1156, 1616, 1855, 1965
BsrFI	RCCGGY	3 cut(s)	217, 1252, 1829
BsrI	ACTGG	9 cut(s)	284, 346, 391, 748, 925, 1711, 1813, 2222, 2947
BssAI	RCCGGY	3 cut(s)	217, 1252, 1829
BssECI	CCNNGG	4 cut(s)	92, 2160, 2478, 2947
BssMI	GATC	8 cut(s)	454, 595, 768, 840, 1099, 1550, 1811, 2983
BssNI	GRCGYC	1 cut(s)	186
BssSI	CACGAG	1 cut(s)	272
BssT1I	CCWWGG	1 cut(s)	2478
Bst2BI	CACGAG	1 cut(s)	272
Bst2UI	CCWGG	5 cut(s)	690, 1146, 2162, 2844, 2948
Bst6I	CTCTTC	5 cut(s)	48, 324, 1201, 2364, 2481
BstACI	GRCGYC	1 cut(s)	186
BstAFI	CTTAAG	1 cut(s)	674
BstBAI	YACGTR	1 cut(s)	2339
BstEII	GGTNACC	1 cut(s)	788
BstF5I	GGATG	4 cut(s)	265, 802, 930, 1540
BstFNI	CGCG	2 cut(s)	77, 228
BstHHI	GCGC	5 cut(s)	79, 228, 1972, 2178, 3004
BstKTI	GATC	8 cut(s)	457, 598, 771, 843, 1102, 1553, 1814, 2986
BstMAI	GTCTC	3 cut(s)	1168, 1820, 2841
BstMBI	GATC	8 cut(s)	454, 595, 768, 840, 1099, 1550, 1811, 2983
BstMCI	CGRYCG	1 cut(s)	297
BstNI	CCWGG	5 cut(s)	690, 1146, 2162, 2844, 2948
BstNSI	RCATGY	3 cut(s)	414, 914, 1996
BstPI	GGTNACC	1 cut(s)	788
BstSCI	CCNGG	8 cut(s)	181, 291, 688, 792, 1144, 2160, 2842, 2946
BstSFI	CTRYAG	3 cut(s)	1020, 2739, 2886
BstUI	CGCG	2 cut(s)	77, 228
BstV2I	GAAGAC	1 cut(s)	979
BstX2I	RGATCY	3 cut(s)	768, 840, 2983
BstXI	CCANNNNNNTGG	2 cut(s)	142, 364
BstYI	RGATCY	3 cut(s)	768, 840, 2983
Bsu15I	ATCGAT	1 cut(s)	598
BsuI	GTATCC	2 cut(s)	2719, 2725
BsuRI	GGCC	5 cut(s)	217, 756, 2351, 2934, 2946
BsuTUI	ATCGAT	1 cut(s)	598
BtgZI	GCGATG	1 cut(s)	2801
BtrI	CACGTC	1 cut(s)	695
BtsCI	GGATG	4 cut(s)	265, 802, 930, 1540
BtsIMutI	CAGTG	5 cut(s)	762, 918, 978, 1265, 1704
BveI	ACCTGC	1 cut(s)	839
CfoI	GCGC	5 cut(s)	79, 228, 1972, 2178, 3004
Cfr10I	RCCGGY	3 cut(s)	217, 1252, 1829
Cfr13I	GGNCC	8 cut(s)	376, 746, 755, 1137, 1675, 2257, 2319, 2897
ClaI	ATCGAT	1 cut(s)	598
CseI	GACGC	1 cut(s)	175
Csp6I	GTAC	6 cut(s)	277, 698, 950, 1250, 1959, 2840
CspAI	ACCGGT	1 cut(s)	1252
CspCI	CAANNNNNGTGG	2 cut(s)	598, 633
CviQI	GTAC	6 cut(s)	277, 698, 950, 1250, 1959, 2840
DpnI	GATC	8 cut(s)	456, 597, 770, 842, 1101, 1552, 1813, 2985
DpnII	GATC	8 cut(s)	454, 595, 768, 840, 1099, 1550, 1811, 2983
DraI	TTTAAA	1 cut(s)	2521
DrdI	GACNNNNNNGTC	1 cut(s)	983
DriI	GACNNNNNGTC	1 cut(s)	2263
DseDI	GACNNNNNNGTC	1 cut(s)	983
EaeI	YGGCCR	2 cut(s)	215, 2944
Eam1104I	CTCTTC	5 cut(s)	48, 324, 1201, 2364, 2481
Eam1105I	GACNNNNNGTC	1 cut(s)	2263
EarI	CTCTTC	5 cut(s)	48, 324, 1201, 2364, 2481
EciI	GGCGGA	2 cut(s)	389, 2687
Eco130I	CCWWGG	1 cut(s)	2478
Eco147I	AGGCCT	1 cut(s)	2351
Eco24I	GRGCYC	1 cut(s)	1030
Eco31I	GGTCTC	1 cut(s)	1820
Eco32I	GATATC	1 cut(s)	2569
Eco47I	GGWCC	7 cut(s)	376, 746, 1137, 1675, 2257, 2319, 2897
Eco57I	CTGAAG	7 cut(s)	238, 938, 1413, 1623, 2388, 2505, 2576
Eco72I	CACGTG	1 cut(s)	2339
Eco88I	CYCGRG	3 cut(s)	245, 836, 1542
Eco91I	GGTNACC	1 cut(s)	788
EcoO109I	RGGNCCY	4 cut(s)	1137, 2257, 2319, 2897
EcoO65I	GGTNACC	1 cut(s)	788
EcoRII	CCWGG	5 cut(s)	688, 1144, 2160, 2842, 2946
EcoRV	GATATC	1 cut(s)	2569
EcoT14I	CCWWGG	1 cut(s)	2478
EcoT22I	ATGCAT	1 cut(s)	2962
EcoT38I	GRGCYC	1 cut(s)	1030
ErhI	CCWWGG	1 cut(s)	2478
Esp3I	CGTCTC	1 cut(s)	1168
FalI	AAGNNNNNCTT	6 cut(s)	707, 739, 928, 960, 2919, 2951
FauI	CCCGC	1 cut(s)	225
FblI	GTMKAC	1 cut(s)	1766
FokI	GGATG	4 cut(s)	272, 809, 937, 1547
FriOI	GRGCYC	1 cut(s)	1030
FspBI	CTAG	5 cut(s)	209, 405, 660, 2531, 2987
FspI	TGCGCA	1 cut(s)	3003
GlaI	GCGC	5 cut(s)	78, 227, 1971, 2177, 3003
GsaI	CCCAGC	1 cut(s)	638
GsuI	CTGGAG	3 cut(s)	1917, 2519, 2856
HaeIII	GGCC	5 cut(s)	217, 756, 2351, 2934, 2946
HapII	CCGG	7 cut(s)	183, 218, 293, 793, 1253, 1286, 1830
HgaI	GACGC	1 cut(s)	175
HhaI	GCGC	5 cut(s)	79, 228, 1972, 2178, 3004
Hin1I	GRCGYC	1 cut(s)	186
Hin6I	GCGC	5 cut(s)	77, 226, 1970, 2176, 3002
HinP1I	GCGC	5 cut(s)	77, 226, 1970, 2176, 3002
HincII	GTYRAC	6 cut(s)	814, 916, 1075, 1945, 2035, 2883
HindII	GTYRAC	6 cut(s)	814, 916, 1075, 1945, 2035, 2883
HindIII	AAGCTT	2 cut(s)	563, 2472
HpaII	CCGG	7 cut(s)	183, 218, 293, 793, 1253, 1286, 1830
HphI	GGTGA	5 cut(s)	118, 635, 749, 1286, 1934
Hpy99I	CGWCG	4 cut(s)	163, 166, 191, 1191
HpyAV	CCTTC	8 cut(s)	250, 1223, 1571, 1900, 2035, 2388, 2767, 2904
HpyCH4IV	ACGT	5 cut(s)	58, 158, 694, 1177, 2338
HpySE526I	ACGT	5 cut(s)	58, 158, 694, 1177, 2338
Hsp92I	GRCGYC	1 cut(s)	186
HspAI	GCGC	5 cut(s)	77, 226, 1970, 2176, 3002
KflI	GGGWCCC	3 cut(s)	1137, 2319, 2897
Kpn2I	TCCGGA	1 cut(s)	1285
KroI	GCCGGC	1 cut(s)	217
KroNI	GCCGGC	1 cut(s)	219
Kzo9I	GATC	8 cut(s)	454, 595, 768, 840, 1099, 1550, 1811, 2983
LmnI	GCTCC	3 cut(s)	125, 649, 1288
MaeI	CTAG	5 cut(s)	209, 405, 660, 2531, 2987
MaeII	ACGT	5 cut(s)	58, 158, 694, 1177, 2338
MaeIII	GTNAC	5 cut(s)	59, 81, 788, 1922, 2407
MalI	GATC	8 cut(s)	456, 597, 770, 842, 1101, 1552, 1813, 2985
MboI	GATC	8 cut(s)	454, 595, 768, 840, 1099, 1550, 1811, 2983
MfeI	CAATTG	2 cut(s)	2106, 2861
MflI	RGATCY	3 cut(s)	768, 840, 2983
MhlI	GDGCHC	1 cut(s)	1030
MlsI	TGGCCA	1 cut(s)	2946
MluNI	TGGCCA	1 cut(s)	2946
MlyI	GAGTC	3 cut(s)	1348, 1465, 1772
MmeI	TCCRAC	1 cut(s)	1030
Mox20I	TGGCCA	1 cut(s)	2946
Mph1103I	ATGCAT	1 cut(s)	2962
MroI	TCCGGA	1 cut(s)	1285
MroNI	GCCGGC	1 cut(s)	217
MroXI	GAANNNNTTC	4 cut(s)	1114, 1167, 1398, 2699
MscI	TGGCCA	1 cut(s)	2946
MseI	TTAA	9 cut(s)	351, 675, 891, 1362, 1692, 2148, 2189, 2436, 2520
MslI	CAYNNNNRTG	6 cut(s)	923, 1592, 1626, 1683, 1991, 2718
Msp20I	TGGCCA	1 cut(s)	2946
MspA1I	CMGCKG	2 cut(s)	1391, 2552
MspCI	CTTAAG	1 cut(s)	674
MspI	CCGG	7 cut(s)	183, 218, 293, 793, 1253, 1286, 1830
MspR9I	CCNGG	8 cut(s)	183, 293, 690, 794, 1146, 2162, 2844, 2948
MunI	CAATTG	2 cut(s)	2106, 2861
Mva1269I	GAATGC	3 cut(s)	1564, 1603, 2784
MvaI	CCWGG	5 cut(s)	690, 1146, 2162, 2844, 2948
MvnI	CGCG	2 cut(s)	77, 228
NaeI	GCCGGC	1 cut(s)	219
NciI	CCSGG	3 cut(s)	183, 293, 794
NdeII	GATC	8 cut(s)	454, 595, 768, 840, 1099, 1550, 1811, 2983
NgoMIV	GCCGGC	1 cut(s)	217
NmuCI	GTSAC	3 cut(s)	81, 1922, 2407
NsbI	TGCGCA	1 cut(s)	3003
NsiI	ATGCAT	1 cut(s)	2962
NspI	RCATGY	3 cut(s)	414, 914, 1996
OliI	CACNNNNGTG	1 cut(s)	1626
PaeI	GCATGC	1 cut(s)	414
PaeR7I	CTCGAG	3 cut(s)	245, 836, 1542
PceI	AGGCCT	1 cut(s)	2351
PciI	ACATGT	1 cut(s)	1992
PcsI	WCGNNNNNNNCGW	3 cut(s)	377, 1183, 1195
PctI	GAATGC	3 cut(s)	1564, 1603, 2784
PdiI	GCCGGC	1 cut(s)	219
PdmI	GAANNNNTTC	4 cut(s)	1114, 1167, 1398, 2699
PfeI	GAWTC	9 cut(s)	9, 97, 103, 109, 115, 191, 2102, 2388, 2447
PflMI	CCANNNNNTGG	2 cut(s)	552, 1040
PinAI	ACCGGT	1 cut(s)	1252
PleI	GAGTC	3 cut(s)	1348, 1465, 1771
PmaCI	CACGTG	1 cut(s)	2339
PmlI	CACGTG	1 cut(s)	2339
PpsI	GAGTC	3 cut(s)	1348, 1465, 1771
Ppu21I	YACGTR	1 cut(s)	2339
PpuMI	RGGWCCY	4 cut(s)	1137, 2257, 2319, 2897
PscI	ACATGT	1 cut(s)	1992
Psp5II	RGGWCCY	4 cut(s)	1137, 2257, 2319, 2897
Psp6I	CCWGG	5 cut(s)	688, 1144, 2160, 2842, 2946
PspCI	CACGTG	1 cut(s)	2339
PspEI	GGTNACC	1 cut(s)	788
PspFI	CCCAGC	1 cut(s)	634
PspGI	CCWGG	5 cut(s)	688, 1144, 2160, 2842, 2946
PspPI	GGNCC	8 cut(s)	376, 746, 755, 1137, 1675, 2257, 2319, 2897
PspPPI	RGGWCCY	4 cut(s)	1137, 2257, 2319, 2897
PspXI	VCTCGAGB	1 cut(s)	245
PstI	CTGCAG	2 cut(s)	2743, 2890
PsuI	RGATCY	3 cut(s)	768, 840, 2983
PvuII	CAGCTG	1 cut(s)	1391
RsaI	GTAC	6 cut(s)	278, 699, 951, 1251, 1960, 2841
RsaNI	GTAC	6 cut(s)	277, 698, 950, 1250, 1959, 2840
RseI	CAYNNNNRTG	6 cut(s)	923, 1592, 1626, 1683, 1991, 2718
SaqAI	TTAA	9 cut(s)	351, 675, 891, 1362, 1692, 2148, 2189, 2436, 2520
Sau3AI	GATC	8 cut(s)	454, 595, 768, 840, 1099, 1550, 1811, 2983
Sau96I	GGNCC	8 cut(s)	376, 746, 755, 1137, 1675, 2257, 2319, 2897
ScaI	AGTACT	1 cut(s)	278
SchI	GAGTC	3 cut(s)	1348, 1465, 1772
ScrFI	CCNGG	8 cut(s)	183, 293, 690, 794, 1146, 2162, 2844, 2948
SduI	GDGCHC	1 cut(s)	1030
SfcI	CTRYAG	3 cut(s)	1020, 2739, 2886
Sfr274I	CTCGAG	3 cut(s)	245, 836, 1542
SinI	GGWCC	7 cut(s)	376, 746, 1137, 1675, 2257, 2319, 2897
SlaI	CTCGAG	3 cut(s)	245, 836, 1542
SmiMI	CAYNNNNRTG	6 cut(s)	923, 1592, 1626, 1683, 1991, 2718
SmlI	CTYRAG	6 cut(s)	245, 674, 836, 1224, 1542, 2453
SmoI	CTYRAG	6 cut(s)	245, 674, 836, 1224, 1542, 2453
SphI	GCATGC	1 cut(s)	414
SseBI	AGGCCT	1 cut(s)	2351
SsiI	CCGC	8 cut(s)	132, 232, 374, 753, 1262, 1846, 2552, 2672
SspMI	CTAG	5 cut(s)	209, 405, 660, 2531, 2987
StuI	AGGCCT	1 cut(s)	2351
StyD4I	CCNGG	8 cut(s)	181, 291, 688, 792, 1144, 2160, 2842, 2946
StyI	CCWWGG	1 cut(s)	2478
TaiI	ACGT	5 cut(s)	61, 161, 697, 1180, 2341
TaqI	TCGA	7 cut(s)	189, 246, 498, 598, 837, 1189, 1543
TatI	WGTACW	3 cut(s)	276, 697, 949
TauI	GCSGC	3 cut(s)	756, 1265, 2555
TfiI	GAWTC	9 cut(s)	9, 97, 103, 109, 115, 191, 2102, 2388, 2447
Tru1I	TTAA	9 cut(s)	351, 675, 891, 1362, 1692, 2148, 2189, 2436, 2520
Tru9I	TTAA	9 cut(s)	351, 675, 891, 1362, 1692, 2148, 2189, 2436, 2520
TscAI	CASTG	5 cut(s)	769, 925, 985, 1272, 1711
TseFI	GTSAC	3 cut(s)	81, 1922, 2407
Tsp45I	GTSAC	3 cut(s)	81, 1922, 2407
TspDTI	ATGAA	9 cut(s)	356, 669, 979, 1107, 1317, 1605, 1610, 1695, 2820
TspGWI	ACGGA	1 cut(s)	1175
TspRI	CASTG	5 cut(s)	769, 925, 985, 1272, 1711
Van91I	CCANNNNNTGG	2 cut(s)	552, 1040
Vha464I	CTTAAG	1 cut(s)	674
VpaK11BI	GGWCC	7 cut(s)	376, 746, 1137, 1675, 2257, 2319, 2897
XapI	RAATTY	5 cut(s)	1376, 1857, 2306, 2544, 2635
XceI	RCATGY	3 cut(s)	414, 914, 1996
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2720
XhoI	CTCGAG	3 cut(s)	245, 836, 1542
XmiI	GTMKAC	1 cut(s)	1766
XmnI	GAANNNNTTC	4 cut(s)	1114, 1167, 1398, 2699
XspI	CTAG	5 cut(s)	209, 405, 660, 2531, 2987
ZrmI	AGTACT	1 cut(s)	278
Zsp2I	ATGCAT	1 cut(s)	2962

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.