Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010393

Physical Location & Seq

Genomic Coordinates Reverse (-)

449663 .. 458697

Gene Structure 9035 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1007000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGAGGAGTGGATTTCGTGAGAACGGAACAATAGCCTTCCGCTTCCTTCACCTCTTACAACCTTACTCTCGTTTCGCTTCTCCCTCTTCTCCCATTCATTTCCCGCCGCCGCGCCTCTCTCGCAGTCGCCCCCTCGCCGCGGGCTACAGGTGCTACTCATCCTCCGCCACGTCCTCGTCTCCCTCCGCCATGGCGGGCGGTGAATCGCAGGAAGTGGAGTGGCCGGCGAAGCGCGTCAGGGACACCTTCGTCAAGTTTTTCGAGGACAAGGGCCACGTCTTCTGGAAGTCTAGCCCCGTCGTTCCTCACAACGATCCCACTCTTTTATTCGCCAATGCTGGTATGAACCAGTTCAAGCCCATTTTTCTGGGCACAGCAGACCCAAACACTCAGTTGAGCAAGCTGACTCGAGCATGCAACACCCAGAAATGTATAAGAGCAGGCGGGAAGCACAACGATCTCGATGATGTAGGGAAAGACACTTACCACCACACTTTCTTTGAGATGCTCGGCAATTGGTCTTTCGGGGATTATTTCAAGAAAGAAGCCATTGGATGGGCTTGGGAGCTTCTCACAAAAGTTTATAAGCTGCCAGAAGATCGAATTTATGCCACCTATTTTGGTGGTGATGAGAAGGCTGGTCTTGCTCCTGATGATGAAGCTAGAGAGTTATGGCTTAAGTTTCTACCAGCCGGCCATGTACTACCGTTTGGCAGTAAGGATAATTTCTGGGAGATGGGCGATACGGGTCCTTGTGGTCCTTGCACAGAAATCCATTACGATAGAATAGGAAATCGTGATGCTGCATCATTAGTCAACAACGATGATCCTACCTGCATTGAAATTTGGAACCTCGTCTTTATTCAGTTCAATAGGGAAAGTGATGGTTCTCTAAAACCTCTCCCTGCTAAGCATGTTGACACTGGGATGGGATTTGAAAGATTAACTTCTATACTTCAGAACAAGATGAGCAATTATGATACTGATGTGTTCATGCCCATCTTTGATGCTATCCAACAGGCAACAGGGGCTCCACCATATTCTGGGAAAGTTGGTGTGGATGATGTAGACAAAATTGACATGGCATACAGAGTTGTTGCCGATCATATAAGAACTCTTTCATTTTCCATTGCTGATGGGTCTCGTCCAGGCAATGACGGTCGTGAATATGTTCTGAGGCGCATTCTTCGTCGTGCTGTTCGGTATGGAAACGATGTACTAAAAGCTAAAGAAGGATTTTTCAATGGGCTTGTACACATTCTGGTGACAGTGATGGGTGATGTTTTTCCAGAGGTACAACAACATGAAGCACATATAAGGGAGGTAATCAAAGAGGAGGAAGAAACTTTTGAGAAGACTTTACAGAAGGGAATAGAAAGATTCAAGAAGGCTGCTCAGGATGTTCAAGGCAAAACATTTAGTGGGCAGGATGCTTTTGTTTTGTGGGACACATTTGGGTTTCCATTAGATTTGACTCAGTTGATGGCAGAGGAAAGGGGATTAATAGTTGATGTTGAGGGTTTCAATAATGCTATGGATGAGGCTCGAGAAAGATCAAGGAGCGCTCAAAATAAGCAAGCTGGTGGTGCTATTGTTATGGATGCTGATGCTACTGCTACATTGCACAAGAGGGGGGTTGCTGCAACAGATGACAGCTTTAAATTTATTTGGAACCAGGACCATGAAAGTGTGGTAAAAGCAATTTATACTGGTTCTGAGTTTATTAATAGTGCTTCTGCTGGCAATGAAGTTGGTATAGTTTTGGAGTCTACAAATTTTTATGCTGAGCAAGGTGGTCAGATTTTTGATACTGGGTCACTTGAAGGATCCTCTCTCTCATTTCAAGTTTGCAATGTTCAAATTTATGGAGGTTTTGTTGTCCACATTGGCTCTTTTTCTGGAGAGGGTGGCAAACTCTTAGTCGGTGACAAAGTAATTTGCAAGGTTGACTACAATAGGCGTAAACTCATTGCGCCCAACCATACCTGCACGCACATGTTGAATTTTGCTCTGAGGGAAATTCTTGGCAATCATGTCGACCAGAAGGGTTCTATTGTTCTTGCTGAGAAATTGAGATTTGATTTTTCACATGGCAAGCCAGTAGATCCTGACAGTTTGAGAAGAATAGAATCAATTGTGAATGAGCAAATCACAGCTGAATTAGGTGTATTTGCTAAGGAGGTAGCCCTGGTTGATGCCAAGCGCATCAATGGTTTAAGGGCTGTATTTGGAGAGGTATATCCCGACCCAGTAAGAGTGGTAGCTATTGGGCGAAAGGTTGAGGACCTTTTGGAATACCCTGAAAATGAGGAATGGTTATCAATTTCAGCAGAACTTTGTGGAGGGACACATATATCAAATACACGTGAAGCAAAGGCCTTTGCTCTTTTATCTGAGGAGGGAATTGCTAAGGGAATACGGAGAATAACTGCTGTCACAAGTGAAGTTGCTTTTCAGGCAATTGAATTGGCTCGGTCGCTTGAGCAAGACGTACATGAAGCGTCCAAGGCTGAAGGAAGCTTGCTGGAGAAGAAAGTAGCTTCCCTAAAATCTCGTGTGGACTCGGCACCAATTCCAGCTGCTAAGAAAGCTGAAATCAGGGCCAAGATGGCTGTGCTTCAGAACCAATTAAAGAAAGCTCAGAAGATGATTGCAGAGCAAAATATAAAGAATGCTGTCAAGGTTGCAACTGAGATGGCTGAAGTTGCTGCTTCTGATGGTAAAGCTTACTGTGTTTCCCAAGTTGATGTTGGTTTGGATGCAGCTGCAGTTCGTGAGGCTGTTTTGAAGATCACTGGGATGGGAATTCCCGCCATGGTTTTCAGCACTGATGAGACCACAAATAAAGCTTTGGTGTGTGCTGGCGTACCAGGGGACAAGGGCACTCAGTTGGAGGTCTCAGAGTGGTTGACAGCAGCTATGGGTCCTTTAAATGGAAGATGCGGAAAAGGAAAAGGTGGCCTTGCTTCTGGCCAGGGAACAGATGCATCTCGTCTAAATGAGGCGATAGACCTTGCAACGAGTTTTGCACAGATGAAACTGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

1016

Amino Acids

111.52

Weight (kDa)

5.89

Isoelectric Point (pI)

37.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	79 - 652	5.1e-221	tRNA synthetases class II (A)
tRNA_SAD	PF07973	751 - 809	4.5e-14	Threonyl and Alanyl tRNA synthetase second additional domain
DHHA1	PF02272	908 - 1010	1.4e-08	DHHA1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50200 AT1G50200
fragaria_vesca	FvH4_4g36780 FvH4_4g36780
malus_domestica	MD13G1007000.v1.1 MD16G1000800.v1.1
prunus_persica	Prupe.1G345900_v2.0.a1
pyrus_communis	pycom13g00590 pycom16g00460
rosa_chinensis	RchiOBHm_Chr4g0446511
rosa_laevigata	RLG00000005653
rosa_multiflora	Rmu_co8401425.1_g000001 Rmu_sc0005526.1_g000002 Rmu_sc0009884.1_g000001
rosa_roxburghii	Rroxscaffold_5G00386930
rosa_rugosa	Rorug04G0372100 Rorug04G0372200
rosa_samantha	Rh4BG442800 Rh4CG460300 Rh4DG441500
rosa_wichuraiana	Rw4G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	585
AasI	GACNNNNNNGTC	1 cut(s)	248
Acc36I	ACCTGC	2 cut(s)	842, 1994
AccB1I	GGYRCC	1 cut(s)	2566
AccB7I	CCANNNNNTGG	2 cut(s)	555, 1043
AccI	GTMKAC	3 cut(s)	1068, 1769, 2037
AccII	CGCG	3 cut(s)	112, 140, 234
AclWI	GGATC	5 cut(s)	308, 821, 1821, 1834, 2099
AcoI	YGGCCR	3 cut(s)	221, 694, 2971
AcsI	RAATTY	8 cut(s)	603, 843, 1661, 1774, 1860, 2002, 2019, 2805
AcuI	CTGAAG	4 cut(s)	941, 2532, 2603, 2721
AcvI	CACGTG	1 cut(s)	2366
AfaI	GTAC	6 cut(s)	702, 1218, 1254, 1296, 2493, 2868
AfeI	AGCGCT	1 cut(s)	1564
AfiI	CCNNNNNNNGG	4 cut(s)	139, 555, 1043, 2933
AflII	CTTAAG	1 cut(s)	677
AflIII	ACRYGT	2 cut(s)	1995, 2363
AjiI	CACGTC	2 cut(s)	171, 277
AjnI	CCWGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
Alw26I	GTCTC	4 cut(s)	183, 1146, 2828, 2902
AlwI	GGATC	5 cut(s)	308, 821, 1821, 1834, 2099
Ama87I	CYCGRG	2 cut(s)	408, 1545
Aor51HI	AGCGCT	1 cut(s)	1564
AoxI	GGCC	7 cut(s)	221, 271, 694, 2376, 2601, 2959, 2971
ApeKI	GCWGC	9 cut(s)	589, 803, 1391, 1640, 2579, 2708, 2762, 2765, 2915
ApoI	RAATTY	8 cut(s)	603, 843, 1661, 1774, 1860, 2002, 2019, 2805
ArsI	GACNNNNNNTTYG	2 cut(s)	1905, 1937
AseI	ATTAAT	2 cut(s)	1502, 1725
Asp700I	GAANNNNTTC	3 cut(s)	350, 1117, 1170
AspLEI	GCGC	6 cut(s)	114, 234, 1182, 1565, 1975, 2205
AspS9I	GGNCC	7 cut(s)	271, 749, 758, 1678, 2284, 2601, 2924
AsuHPI	GGTGA	6 cut(s)	41, 212, 638, 1276, 1289, 1937
AvaI	CYCGRG	2 cut(s)	408, 1545
AvaII	GGWCC	5 cut(s)	749, 758, 1678, 2284, 2924
BaeGI	GKGCMC	2 cut(s)	374, 2885
BaeI	ACNNNNGTAYC	2 cut(s)	972, 1005
BalI	TGGCCA	1 cut(s)	2973
BamHI	GGATCC	1 cut(s)	1826
BanI	GGYRCC	1 cut(s)	2566
BanII	GRGCYC	1 cut(s)	1033
BauI	CACGAG	1 cut(s)	2553
BbrPI	CACGTG	1 cut(s)	2366
BbsI	GAAGAC	2 cut(s)	271, 1361
BbvI	GCAGC	9 cut(s)	576, 790, 1378, 1627, 2566, 2695, 2752, 2774, 2927
BcgI	CGANNNNNNTGC	2 cut(s)	2017, 2051
BciT130I	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
BcoDI	GTCTC	4 cut(s)	183, 1146, 2828, 2902
BfaI	CTAG	2 cut(s)	291, 663
BfmI	CTRYAG	2 cut(s)	145, 2766
BfoI	RGCGCY	1 cut(s)	1566
BfrI	CTTAAG	1 cut(s)	677
BfuAI	ACCTGC	2 cut(s)	842, 1994
BglI	GCCNNNNNGGC	1 cut(s)	2609
BlpI	GCTNAGC	2 cut(s)	909, 1785
Bme1390I	CCNGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
Bme18I	GGWCC	5 cut(s)	749, 758, 1678, 2284, 2924
BmeT110I	CYCGRG	2 cut(s)	408, 1545
BmgBI	CACGTC	2 cut(s)	171, 277
BmgT120I	GGNCC	7 cut(s)	271, 749, 758, 1678, 2284, 2601, 2924
BmiI	GGNNCC	7 cut(s)	750, 851, 1032, 1673, 1828, 2568, 2925
BmrFI	CCNGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
BmrI	ACTGGG	4 cut(s)	933, 1821, 2243, 2805
BmuI	ACTGGG	4 cut(s)	933, 1821, 2243, 2805
BpiI	GAAGAC	2 cut(s)	271, 1361
BpmI	CTGGAG	2 cut(s)	1920, 2546
Bpu10I	CCTNAGC	3 cut(s)	1395, 2175, 2409
Bpu1102I	GCTNAGC	2 cut(s)	909, 1785
BpuEI	CTTGAG	1 cut(s)	2501
BsaAI	YACGTR	1 cut(s)	2366
BsaI	GGTCTC	3 cut(s)	1146, 2828, 2902
BsaJI	CCNNGG	7 cut(s)	138, 189, 2187, 2505, 2814, 2870, 2974
BsaXI	ACNNNNNCTCC	6 cut(s)	146, 176, 1015, 1045, 1860, 1890
Bsc4I	CCNNNNNNNGG	4 cut(s)	139, 555, 1043, 2933
Bse118I	RCCGGY	2 cut(s)	223, 692
Bse1I	ACTGG	7 cut(s)	349, 928, 1714, 1816, 2099, 2249, 2800
Bse3DI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1858, 1968
BseBI	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
BseDI	CCNNGG	7 cut(s)	138, 189, 2187, 2505, 2814, 2870, 2974
BseLI	CCNNNNNNNGG	4 cut(s)	139, 555, 1043, 2933
BseMI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1858, 1968
BseNI	ACTGG	7 cut(s)	349, 928, 1714, 1816, 2099, 2249, 2800
BseRI	GAGGAG	3 cut(s)	19, 1349, 2411
BseSI	GKGCMC	2 cut(s)	374, 2885
BseXI	GCAGC	9 cut(s)	576, 790, 1378, 1627, 2566, 2695, 2752, 2774, 2927
BsgI	GTGCAG	1 cut(s)	1972
Bsh1236I	CGCG	3 cut(s)	112, 140, 234
Bsh1285I	CGRYCG	2 cut(s)	1162, 2477
BshFI	GGCC	7 cut(s)	223, 273, 696, 2378, 2603, 2961, 2973
BshNI	GGYRCC	1 cut(s)	2566
BsiEI	CGRYCG	2 cut(s)	1162, 2477
BsiHKCI	CYCGRG	2 cut(s)	408, 1545
BsiSI	CCGG	2 cut(s)	224, 693
BslFI	GGGAC	4 cut(s)	254, 1460, 2359, 2888
BslI	CCNNNNNNNGG	4 cut(s)	139, 555, 1043, 2933
BsmAI	GTCTC	4 cut(s)	183, 1146, 2828, 2902
BsmBI	CGTCTC	1 cut(s)	183
BsmFI	GGGAC	4 cut(s)	254, 1460, 2359, 2888
BsmI	GAATGC	2 cut(s)	1182, 2677
BsnI	GGCC	7 cut(s)	223, 273, 696, 2378, 2603, 2961, 2973
Bso31I	GGTCTC	3 cut(s)	1146, 2828, 2902
BsoBI	CYCGRG	2 cut(s)	408, 1545
Bsp1286I	GDGCHC	3 cut(s)	374, 1033, 2885
Bsp1407I	TGTACA	1 cut(s)	1252
Bsp143I	GATC	9 cut(s)	313, 457, 598, 826, 1102, 1553, 1826, 2104, 2790
Bsp1720I	GCTNAGC	2 cut(s)	909, 1785
Bsp19I	CCATGG	2 cut(s)	189, 2814
BspANI	GGCC	7 cut(s)	223, 273, 696, 2378, 2603, 2961, 2973
BspFNI	CGCG	3 cut(s)	112, 140, 234
BspLI	GGNNCC	7 cut(s)	750, 851, 1032, 1673, 1828, 2568, 2925
BspMAI	CTGCAG	1 cut(s)	2770
BspMI	ACCTGC	2 cut(s)	842, 1994
BspPI	GGATC	5 cut(s)	308, 821, 1821, 1834, 2099
BspT107I	GGYRCC	1 cut(s)	2566
BspTI	CTTAAG	1 cut(s)	677
BspTNI	GGTCTC	3 cut(s)	1146, 2828, 2902
BsrDI	GCAATG	6 cut(s)	1128, 1159, 1619, 1750, 1858, 1968
BsrFI	RCCGGY	2 cut(s)	223, 692
BsrGI	TGTACA	1 cut(s)	1252
BsrI	ACTGG	7 cut(s)	349, 928, 1714, 1816, 2099, 2249, 2800
BssAI	RCCGGY	2 cut(s)	223, 692
BssECI	CCNNGG	7 cut(s)	138, 189, 2187, 2505, 2814, 2870, 2974
BssMI	GATC	9 cut(s)	313, 457, 598, 826, 1102, 1553, 1826, 2104, 2790
BssSI	CACGAG	1 cut(s)	2553
BssT1I	CCWWGG	3 cut(s)	189, 2505, 2814
Bst2BI	CACGAG	1 cut(s)	2553
Bst2UI	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
Bst4CI	ACNGT	5 cut(s)	708, 1160, 1270, 2114, 2732
Bst6I	CTCTTC	1 cut(s)	91
BstAFI	CTTAAG	1 cut(s)	677
BstAUI	TGTACA	1 cut(s)	1252
BstBAI	YACGTR	1 cut(s)	2366
BstDSI	CCRYGG	3 cut(s)	138, 189, 2814
BstFNI	CGCG	3 cut(s)	112, 140, 234
BstH2I	RGCGCY	1 cut(s)	1566
BstHHI	GCGC	6 cut(s)	114, 234, 1182, 1565, 1975, 2205
BstKTI	GATC	9 cut(s)	316, 460, 601, 829, 1105, 1556, 1829, 2107, 2793
BstMAI	GTCTC	4 cut(s)	183, 1146, 2828, 2902
BstMBI	GATC	9 cut(s)	313, 457, 598, 826, 1102, 1553, 1826, 2104, 2790
BstMCI	CGRYCG	2 cut(s)	1162, 2477
BstNI	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
BstNSI	RCATGY	3 cut(s)	417, 917, 1999
BstSCI	CCNGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
BstSFI	CTRYAG	2 cut(s)	145, 2766
BstSLI	GKGCMC	2 cut(s)	374, 2885
BstUI	CGCG	3 cut(s)	112, 140, 234
BstV1I	GCAGC	9 cut(s)	576, 790, 1378, 1627, 2566, 2695, 2752, 2774, 2927
BstV2I	GAAGAC	2 cut(s)	271, 1361
BstX2I	RGATCY	2 cut(s)	1826, 2104
BstXI	CCANNNNNNTGG	1 cut(s)	367
BstYI	RGATCY	2 cut(s)	1826, 2104
BsuRI	GGCC	7 cut(s)	223, 273, 696, 2378, 2603, 2961, 2973
BtgI	CCRYGG	3 cut(s)	138, 189, 2814
BtrI	CACGTC	2 cut(s)	171, 277
BtsIMutI	CAGTG	4 cut(s)	921, 1275, 2793, 2826
BveI	ACCTGC	2 cut(s)	842, 1994
CfoI	GCGC	6 cut(s)	114, 234, 1182, 1565, 1975, 2205
Cfr10I	RCCGGY	2 cut(s)	223, 692
Cfr13I	GGNCC	7 cut(s)	271, 749, 758, 1678, 2284, 2601, 2924
Cfr42I	CCGCGG	1 cut(s)	141
CseI	GACGC	2 cut(s)	223, 2490
Csp6I	GTAC	6 cut(s)	701, 1217, 1253, 1295, 2492, 2867
CspCI	CAANNNNNGTGG	2 cut(s)	601, 636
CviQI	GTAC	6 cut(s)	701, 1217, 1253, 1295, 2492, 2867
DpnI	GATC	9 cut(s)	315, 459, 600, 828, 1104, 1555, 1828, 2106, 2792
DpnII	GATC	9 cut(s)	313, 457, 598, 826, 1102, 1553, 1826, 2104, 2790
DraI	TTTAAA	2 cut(s)	1660, 2931
DrdI	GACNNNNNNGTC	1 cut(s)	248
DseDI	GACNNNNNNGTC	1 cut(s)	248
EaeI	YGGCCR	3 cut(s)	221, 694, 2971
Eam1104I	CTCTTC	1 cut(s)	91
EarI	CTCTTC	1 cut(s)	91
EciI	GGCGGA	2 cut(s)	154, 175
Eco130I	CCWWGG	3 cut(s)	189, 2505, 2814
Eco147I	AGGCCT	1 cut(s)	2378
Eco24I	GRGCYC	1 cut(s)	1033
Eco31I	GGTCTC	3 cut(s)	1146, 2828, 2902
Eco47I	GGWCC	5 cut(s)	749, 758, 1678, 2284, 2924
Eco47III	AGCGCT	1 cut(s)	1564
Eco57I	CTGAAG	4 cut(s)	941, 2532, 2603, 2721
Eco72I	CACGTG	1 cut(s)	2366
Eco88I	CYCGRG	2 cut(s)	408, 1545
EcoO109I	RGGNCCY	3 cut(s)	749, 2284, 2924
EcoRI	GAATTC	1 cut(s)	2805
EcoRII	CCWGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
EcoT14I	CCWWGG	3 cut(s)	189, 2505, 2814
EcoT22I	ATGCAT	1 cut(s)	2989
EcoT38I	GRGCYC	1 cut(s)	1033
ErhI	CCWWGG	3 cut(s)	189, 2505, 2814
Esp3I	CGTCTC	1 cut(s)	183
FalI	AAGNNNNNCTT	4 cut(s)	931, 963, 2946, 2978
FaqI	GGGAC	4 cut(s)	254, 1460, 2359, 2888
FauI	CCCGC	5 cut(s)	111, 133, 187, 437, 2818
FblI	GTMKAC	3 cut(s)	1068, 1769, 2037
FriOI	GRGCYC	1 cut(s)	1033
FspBI	CTAG	2 cut(s)	291, 663
GlaI	GCGC	6 cut(s)	113, 233, 1181, 1564, 1974, 2204
GsuI	CTGGAG	2 cut(s)	1920, 2546
HaeII	RGCGCY	1 cut(s)	1566
HaeIII	GGCC	7 cut(s)	223, 273, 696, 2378, 2603, 2961, 2973
HapII	CCGG	2 cut(s)	224, 693
HgaI	GACGC	2 cut(s)	223, 2490
HhaI	GCGC	6 cut(s)	114, 234, 1182, 1565, 1975, 2205
Hin6I	GCGC	6 cut(s)	112, 232, 1180, 1563, 1973, 2203
HinP1I	GCGC	6 cut(s)	112, 232, 1180, 1563, 1973, 2203
HincII	GTYRAC	5 cut(s)	817, 919, 1948, 2038, 2910
HindII	GTYRAC	5 cut(s)	817, 919, 1948, 2038, 2910
HindIII	AAGCTT	3 cut(s)	2518, 2724, 2847
HinfI	GANTC	7 cut(s)	203, 406, 1380, 1474, 1766, 2129, 2561
HpaII	CCGG	2 cut(s)	224, 693
HphI	GGTGA	6 cut(s)	41, 212, 638, 1276, 1289, 1937
Hpy99I	CGWCG	2 cut(s)	302, 1194
HpyCH4III	ACNGT	5 cut(s)	708, 1160, 1270, 2114, 2732
HpyCH4IV	ACGT	4 cut(s)	170, 276, 2365, 2490
HpySE526I	ACGT	4 cut(s)	170, 276, 2365, 2490
HspAI	GCGC	6 cut(s)	112, 232, 1180, 1563, 1973, 2203
KroI	GCCGGC	2 cut(s)	223, 692
KroNI	GCCGGC	2 cut(s)	225, 694
KspI	CCGCGG	1 cut(s)	141
Kzo9I	GATC	9 cut(s)	313, 457, 598, 826, 1102, 1553, 1826, 2104, 2790
LmnI	GCTCC	4 cut(s)	565, 652, 1036, 1560
Lsp1109I	GCAGC	9 cut(s)	576, 790, 1378, 1627, 2566, 2695, 2752, 2774, 2927
MaeI	CTAG	2 cut(s)	291, 663
MaeII	ACGT	4 cut(s)	170, 276, 2365, 2490
MaeIII	GTNAC	4 cut(s)	1264, 1815, 1925, 2434
MalI	GATC	9 cut(s)	315, 459, 600, 828, 1104, 1555, 1828, 2106, 2792
MboI	GATC	9 cut(s)	313, 457, 598, 826, 1102, 1553, 1826, 2104, 2790
MfeI	CAATTG	3 cut(s)	514, 2133, 2460
MflI	RGATCY	2 cut(s)	1826, 2104
MhlI	GDGCHC	3 cut(s)	374, 1033, 2885
MlsI	TGGCCA	1 cut(s)	2973
MluNI	TGGCCA	1 cut(s)	2973
MlyI	GAGTC	4 cut(s)	400, 1468, 1775, 2555
MmeI	TCCRAC	2 cut(s)	1039, 2871
Mox20I	TGGCCA	1 cut(s)	2973
Mph1103I	ATGCAT	1 cut(s)	2989
MroNI	GCCGGC	2 cut(s)	223, 692
MroXI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
MscI	TGGCCA	1 cut(s)	2973
MseI	TTAA	8 cut(s)	678, 944, 1502, 1659, 1725, 2216, 2630, 2930
MslI	CAYNNNNRTG	5 cut(s)	926, 1262, 1686, 1994, 2798
Msp20I	TGGCCA	1 cut(s)	2973
MspA1I	CMGCKG	4 cut(s)	140, 2156, 2579, 2765
MspCI	CTTAAG	1 cut(s)	677
MspI	CCGG	2 cut(s)	224, 693
MspR9I	CCNGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
MunI	CAATTG	3 cut(s)	514, 2133, 2460
Mva1269I	GAATGC	2 cut(s)	1182, 2677
MvaI	CCWGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
MvnI	CGCG	3 cut(s)	112, 140, 234
NaeI	GCCGGC	2 cut(s)	225, 694
NcoI	CCATGG	2 cut(s)	189, 2814
NdeII	GATC	9 cut(s)	313, 457, 598, 826, 1102, 1553, 1826, 2104, 2790
NgoMIV	GCCGGC	2 cut(s)	223, 692
NlaIV	GGNNCC	7 cut(s)	750, 851, 1032, 1673, 1828, 2568, 2925
NmeAIII	GCCGAG	2 cut(s)	489, 2543
NmuCI	GTSAC	4 cut(s)	1264, 1815, 1925, 2434
NsiI	ATGCAT	1 cut(s)	2989
NspI	RCATGY	3 cut(s)	417, 917, 1999
PaeI	GCATGC	1 cut(s)	417
PaeR7I	CTCGAG	2 cut(s)	408, 1545
PceI	AGGCCT	1 cut(s)	2378
PciI	ACATGT	1 cut(s)	1995
PctI	GAATGC	2 cut(s)	1182, 2677
PdiI	GCCGGC	2 cut(s)	225, 694
PdmI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
PfeI	GAWTC	3 cut(s)	203, 1380, 2129
PflMI	CCANNNNNTGG	2 cut(s)	555, 1043
PleI	GAGTC	4 cut(s)	400, 1468, 1774, 2555
PmaCI	CACGTG	1 cut(s)	2366
PmlI	CACGTG	1 cut(s)	2366
PpsI	GAGTC	4 cut(s)	400, 1468, 1774, 2555
Ppu21I	YACGTR	1 cut(s)	2366
PpuMI	RGGWCCY	3 cut(s)	749, 2284, 2924
PscI	ACATGT	1 cut(s)	1995
PshBI	ATTAAT	2 cut(s)	1502, 1725
PsiI	TTATAA	1 cut(s)	585
Psp5II	RGGWCCY	3 cut(s)	749, 2284, 2924
Psp6I	CCWGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
PspCI	CACGTG	1 cut(s)	2366
PspGI	CCWGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
PspN4I	GGNNCC	7 cut(s)	750, 851, 1032, 1673, 1828, 2568, 2925
PspPI	GGNCC	7 cut(s)	271, 749, 758, 1678, 2284, 2601, 2924
PspPPI	RGGWCCY	3 cut(s)	749, 2284, 2924
PspXI	VCTCGAGB	1 cut(s)	408
PstI	CTGCAG	1 cut(s)	2770
PsuI	RGATCY	2 cut(s)	1826, 2104
PvuII	CAGCTG	3 cut(s)	2156, 2579, 2765
RsaI	GTAC	6 cut(s)	702, 1218, 1254, 1296, 2493, 2868
RsaNI	GTAC	6 cut(s)	701, 1217, 1253, 1295, 2492, 2867
RseI	CAYNNNNRTG	5 cut(s)	926, 1262, 1686, 1994, 2798
SacII	CCGCGG	1 cut(s)	141
SalI	GTCGAC	1 cut(s)	2036
SaqAI	TTAA	8 cut(s)	678, 944, 1502, 1659, 1725, 2216, 2630, 2930
Sau3AI	GATC	9 cut(s)	313, 457, 598, 826, 1102, 1553, 1826, 2104, 2790
Sau96I	GGNCC	7 cut(s)	271, 749, 758, 1678, 2284, 2601, 2924
SchI	GAGTC	4 cut(s)	400, 1468, 1775, 2555
ScrFI	CCNGG	5 cut(s)	1149, 1676, 2189, 2871, 2975
SduI	GDGCHC	3 cut(s)	374, 1033, 2885
SfcI	CTRYAG	2 cut(s)	145, 2766
Sfr274I	CTCGAG	2 cut(s)	408, 1545
Sfr303I	CCGCGG	1 cut(s)	141
SgrBI	CCGCGG	1 cut(s)	141
SinI	GGWCC	5 cut(s)	749, 758, 1678, 2284, 2924
SlaI	CTCGAG	2 cut(s)	408, 1545
SmiMI	CAYNNNNRTG	5 cut(s)	926, 1262, 1686, 1994, 2798
SmlI	CTYRAG	4 cut(s)	408, 677, 1545, 2480
SmoI	CTYRAG	4 cut(s)	408, 677, 1545, 2480
SphI	GCATGC	1 cut(s)	417
SseBI	AGGCCT	1 cut(s)	2378
SspMI	CTAG	2 cut(s)	291, 663
StuI	AGGCCT	1 cut(s)	2378
StyD4I	CCNGG	5 cut(s)	1147, 1674, 2187, 2869, 2973
StyI	CCWWGG	3 cut(s)	189, 2505, 2814
TaaI	ACNGT	5 cut(s)	708, 1160, 1270, 2114, 2732
TaiI	ACGT	4 cut(s)	173, 279, 2368, 2493
TaqI	TCGA	6 cut(s)	261, 409, 462, 601, 1546, 2037
TaqII	GACCGA	1 cut(s)	2463
TatI	WGTACW	3 cut(s)	700, 1216, 1252
TauI	GCSGC	3 cut(s)	109, 112, 140
TfiI	GAWTC	3 cut(s)	203, 1380, 2129
Tru1I	TTAA	8 cut(s)	678, 944, 1502, 1659, 1725, 2216, 2630, 2930
Tru9I	TTAA	8 cut(s)	678, 944, 1502, 1659, 1725, 2216, 2630, 2930
TscAI	CASTG	4 cut(s)	928, 1275, 2800, 2833
TseFI	GTSAC	4 cut(s)	1264, 1815, 1925, 2434
TseI	GCWGC	9 cut(s)	589, 803, 1391, 1640, 2579, 2708, 2762, 2765, 2915
Tsp45I	GTSAC	4 cut(s)	1264, 1815, 1925, 2434
TspDTI	ATGAA	9 cut(s)	86, 359, 672, 982, 1110, 1320, 1698, 1761, 2511
TspGWI	ACGGA	2 cut(s)	39, 2434
TspRI	CASTG	4 cut(s)	928, 1275, 2800, 2833
Van91I	CCANNNNNTGG	2 cut(s)	555, 1043
Vha464I	CTTAAG	1 cut(s)	677
VpaK11BI	GGWCC	5 cut(s)	749, 758, 1678, 2284, 2924
VspI	ATTAAT	2 cut(s)	1502, 1725
XapI	RAATTY	8 cut(s)	603, 843, 1661, 1774, 1860, 2002, 2019, 2805
XceI	RCATGY	3 cut(s)	417, 917, 1999
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2747
XhoI	CTCGAG	2 cut(s)	408, 1545
XmiI	GTMKAC	3 cut(s)	1068, 1769, 2037
XmnI	GAANNNNTTC	3 cut(s)	350, 1117, 1170
XspI	CTAG	2 cut(s)	291, 663
Zsp2I	ATGCAT	1 cut(s)	2989

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.