AT1G52630

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008507
Physical Location & Seq
Reverse (-)
19606335 .. 19608648
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G52630.1

Sequence Viewer

Length: 1320 bp
ATGATTGGATCTCCGGTGAAACCACTGTTCGTCTTCGTCTTAACTTTCTCTCTTCTCCTCGTCGTTATTCTTCTATCCCCGTCGCCACATATTCTGCAGATTCCGTTTCCTTCCGGTTCATCAGTGGGTTCTTCTGATATATGGAGTGTGAAGAGGATAATGGAATGGAGACCTTGCAAATGGTGGTTACAAGGACACCTCACTCCTCTACCTGCTAAAACTAATGGATATATACGCGTTGATTGCTATGGCGGCCTGAATCAGATGAGACGCGATTTGTGTGATGGAGTTGGGATTGCCCGTTTGTTGAATGCTACACTTGTTCTGCCAAAGTTTGAGGTAGCTGCCTATTGGAATGAGTCAAGTGGATTTGCAGATGTGTTTGATGTAGACTACTTTATTCAAAAGATGAGTGGTTATATCGAAGTTGTTAAAGAATTGCCAAAAGATATTGCATCAAAAGAACCATTCAAAGTAGACTGCAGCAAAAGGAAGGGTCAATTTGATTACATTGAAAGTGTTCTTCCACTGTTGTTGGAACATCATTACATTTCTTTTACACCAGCAATGAGCCAACGCAGGGACAGGTATCCCGAATATGCAAGAGCCACTCTGTGTCAAGCCTGTTATTCGGCTATACACCTTACTAGTTCCTTGGAGAAGAAGGCCGTCGAGCTTTTTGATGCTATTCCCAAACCCTTCTTGTCGCTTCACCTCAGATTTGAACCTGACATGGTAGCGTACAGCCAATGTGAATACCCAAACCTCTCTCCTTCATCTATCGCTGCCATTGAAGCTGCTCGTGCTGATAGGAAGCCATGGACTGGAGAACTAGCTCAGACATGGAGAAAGCGAGGCAAATGCCCTCTGACTCCTAATGAAACAGTGTTGATGCTTCAGTCACTTAACATCCCAACAAGCACAAACATATACTTAGCAGCTGGAGACGGTCTAATGGAGATGGAAGGATTCACATCCGTTTATACAAATGTGTTCACAAAGTCTGTTCTACTTAACCAAGAGGATTTCACCAGAATGCATGGAAACACAAAAGCTGCATTGGATTATCACGTGTCCATCAATAGTGACGCATATGTGGCTACGTACTTTGGAAACATGGATAAAATAGTTGCAGCTATGCGAACATATAAACAGATGCATAATACACTGTTCCTGAGCAGAAAAGCATTTGCGGAGCTCACTTCTCAGGGTCTTGAAGGTGCAGAGTTGAAGAAAGCTCTCTGGGAAGTTCATAAAAGCGATTTTGCCATTGGCAGAGGATTTGCTTTGCCCGACTGCTTCTGTGAATTTGAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

439

Amino Acids

49.55

Weight (kDa)

7.12

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 77 - 383 1.4e-62 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 220
AccB7I CCANNNNNTGG 1 cut(s) 824
AccI GTMKAC 2 cut(s) 390, 477
AccII CGCG 2 cut(s) 237, 273
AciI CCGC 2 cut(s) 252, 1193
AclWI GGATC 1 cut(s) 16
AcsI RAATTY 1 cut(s) 1307
AcuI CTGAAG 1 cut(s) 881
AcvI CACGTG 1 cut(s) 1072
AdeI CACNNNGTG 1 cut(s) 615
AfaI GTAC 2 cut(s) 743, 1106
AfiI CCNNNNNNNGG 2 cut(s) 580, 824
AflIII ACRYGT 2 cut(s) 235, 1071
AgsI TTSAA 8 cut(s) 310, 404, 472, 515, 725, 794, 1217, 1231
AhlI ACTAGT 1 cut(s) 647
Alw21I GWGCWC 1 cut(s) 1200
Alw26I GTCTC 3 cut(s) 163, 262, 939
AlwI GGATC 1 cut(s) 16
AoxI GGCC 2 cut(s) 253, 666
ApeKI GCWGC 7 cut(s) 344, 483, 785, 797, 938, 1055, 1133
ApoI RAATTY 1 cut(s) 1307
Asp700I GAANNNNTTC 1 cut(s) 519
AsuHPI GGTGA 3 cut(s) 28, 704, 1021
BanII GRGCYC 1 cut(s) 1200
BauI CACGAG 1 cut(s) 801
BbrPI CACGTG 1 cut(s) 1072
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 1200
BbvI GCAGC 7 cut(s) 331, 495, 772, 784, 950, 1042, 1145
BccI CCATC 3 cut(s) 278, 955, 1085
BceAI ACGGC 1 cut(s) 653
BcgI CGANNNNNNTGC 2 cut(s) 843, 877
BciVI GTATCC 1 cut(s) 600
BcoDI GTCTC 3 cut(s) 163, 262, 939
BcuI ACTAGT 1 cut(s) 647
BfaI CTAG 2 cut(s) 648, 833
BfmI CTRYAG 2 cut(s) 95, 481
BfuAI ACCTGC 1 cut(s) 220
BfuI GTATCC 1 cut(s) 600
BisI GCNGC 8 cut(s) 253, 345, 484, 786, 798, 939, 1056, 1134
BlsI GCNGC 8 cut(s) 254, 346, 485, 787, 799, 940, 1057, 1135
BmsI GCATC 4 cut(s) 464, 673, 882, 1146
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 2 cut(s) 846, 963
Bpu10I CCTNAGC 1 cut(s) 1175
BsaAI YACGTR 2 cut(s) 1072, 1104
BsaBI GATNNNNATC 1 cut(s) 973
BsaI GGTCTC 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 654, 818
BsaWI WCCGGW 2 cut(s) 13, 113
Bsc4I CCNNNNNNNGG 2 cut(s) 580, 824
Bse1I ACTGG 1 cut(s) 829
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 1 cut(s) 973
BseDI CCNNGG 2 cut(s) 654, 818
BseGI GGATG 2 cut(s) 909, 974
BseJI GATNNNNATC 1 cut(s) 973
BseLI CCNNNNNNNGG 2 cut(s) 580, 824
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 4 cut(s) 730, 851, 1166, 1220
BseNI ACTGG 1 cut(s) 829
BseRI GAGGAG 2 cut(s) 47, 195
BseXI GCAGC 7 cut(s) 331, 495, 772, 784, 950, 1042, 1145
BsgI GTGCAG 1 cut(s) 1242
Bsh1236I CGCG 2 cut(s) 237, 273
BshFI GGCC 2 cut(s) 255, 668
BsiHKAI GWGCWC 1 cut(s) 1200
BsiSI CCGG 2 cut(s) 14, 114
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 2 cut(s) 580, 824
BsmAI GTCTC 3 cut(s) 163, 262, 939
BsmBI CGTCTC 2 cut(s) 262, 939
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 2 cut(s) 316, 1041
BsnI GGCC 2 cut(s) 255, 668
Bso31I GGTCTC 1 cut(s) 163
Bsp1286I GDGCHC 1 cut(s) 1200
Bsp143I GATC 1 cut(s) 8
Bsp19I CCATGG 1 cut(s) 818
BspACI CCGC 2 cut(s) 252, 1193
BspANI GGCC 2 cut(s) 255, 668
BspCNI CTCAG 4 cut(s) 729, 850, 1167, 1219
BspFNI CGCG 2 cut(s) 237, 273
BspMAI CTGCAG 2 cut(s) 99, 485
BspMI ACCTGC 1 cut(s) 220
BspPI GGATC 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 1 cut(s) 829
BssECI CCNNGG 2 cut(s) 654, 818
BssMI GATC 1 cut(s) 8
BssSI CACGAG 1 cut(s) 801
BssT1I CCWWGG 2 cut(s) 654, 818
Bst2BI CACGAG 1 cut(s) 801
Bst4CI ACNGT 5 cut(s) 27, 531, 886, 950, 1170
Bst6I CTCTTC 2 cut(s) 57, 146
BstBAI YACGTR 2 cut(s) 1072, 1104
BstDEI CTNAG 5 cut(s) 716, 837, 934, 1175, 1206
BstDSI CCRYGG 1 cut(s) 818
BstF5I GGATG 2 cut(s) 909, 974
BstFNI CGCG 2 cut(s) 237, 273
BstKTI GATC 1 cut(s) 11
BstMAI GTCTC 3 cut(s) 163, 262, 939
BstMBI GATC 1 cut(s) 8
BstMWI GCNNNNNNNGC 5 cut(s) 243, 252, 794, 803, 1097
BstSFI CTRYAG 2 cut(s) 95, 481
BstSNI TACGTA 1 cut(s) 1104
BstUI CGCG 2 cut(s) 237, 273
BstV1I GCAGC 7 cut(s) 331, 495, 772, 784, 950, 1042, 1145
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuI GTATCC 1 cut(s) 600
BsuRI GGCC 2 cut(s) 255, 668
BtgI CCRYGG 1 cut(s) 818
BtsCI GGATG 2 cut(s) 909, 974
BtsIMutI CAGTG 5 cut(s) 23, 129, 527, 891, 1166
BveI ACCTGC 1 cut(s) 220
CseI GACGC 2 cut(s) 279, 1097
Csp6I GTAC 2 cut(s) 742, 1105
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 5 cut(s) 733, 819, 843, 1040, 1117
CviQI GTAC 2 cut(s) 742, 1105
DdeI CTNAG 5 cut(s) 716, 837, 934, 1175, 1206
DpnI GATC 1 cut(s) 10
DpnII GATC 1 cut(s) 8
DraIII CACNNNGTG 1 cut(s) 615
Eam1104I CTCTTC 2 cut(s) 57, 146
EarI CTCTTC 2 cut(s) 57, 146
Ecl136II GAGCTC 1 cut(s) 1198
Eco105I TACGTA 1 cut(s) 1104
Eco130I CCWWGG 2 cut(s) 654, 818
Eco24I GRGCYC 1 cut(s) 1200
Eco31I GGTCTC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 1198
Eco57I CTGAAG 1 cut(s) 881
Eco72I CACGTG 1 cut(s) 1072
EcoICRI GAGCTC 1 cut(s) 1198
EcoT14I CCWWGG 2 cut(s) 654, 818
EcoT22I ATGCAT 2 cut(s) 1041, 1161
EcoT38I GRGCYC 1 cut(s) 1200
ErhI CCWWGG 2 cut(s) 654, 818
Esp3I CGTCTC 2 cut(s) 262, 939
FaeI CATG 5 cut(s) 736, 822, 846, 1043, 1120
FaqI GGGAC 1 cut(s) 596
FatI CATG 5 cut(s) 732, 818, 842, 1039, 1116
FauNDI CATATG 1 cut(s) 1093
FblI GTMKAC 2 cut(s) 390, 477
Fnu4HI GCNGC 8 cut(s) 253, 345, 484, 786, 798, 939, 1056, 1134
FokI GGATG 2 cut(s) 896, 961
FriOI GRGCYC 1 cut(s) 1200
Fsp4HI GCNGC 8 cut(s) 253, 345, 484, 786, 798, 939, 1056, 1134
FspBI CTAG 2 cut(s) 648, 833
GluI GCNGC 8 cut(s) 253, 345, 484, 786, 798, 939, 1056, 1134
GsuI CTGGAG 2 cut(s) 846, 963
HaeIII GGCC 2 cut(s) 255, 668
HapII CCGG 2 cut(s) 14, 114
HgaI GACGC 2 cut(s) 279, 1097
Hin1II CATG 5 cut(s) 736, 822, 846, 1043, 1120
HinfI GANTC 5 cut(s) 100, 259, 359, 871, 969
HpaII CCGG 2 cut(s) 14, 114
HphI GGTGA 3 cut(s) 28, 704, 1021
Hpy166II GTNNAC 3 cut(s) 391, 478, 996
Hpy188I TCNGA 5 cut(s) 136, 264, 719, 840, 870
Hpy188III TCNNGA 3 cut(s) 593, 1174, 1214
Hpy8I GTNNAC 3 cut(s) 391, 478, 996
Hpy99I CGWCG 3 cut(s) 65, 85, 674
HpyAV CCTTC 7 cut(s) 120, 487, 658, 709, 783, 959, 1211
HpyCH4III ACNGT 5 cut(s) 27, 531, 886, 950, 1170
HpyCH4IV ACGT 2 cut(s) 1071, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 243, 252, 794, 803, 1097
HpyF3I CTNAG 5 cut(s) 716, 837, 934, 1175, 1206
HpySE526I ACGT 2 cut(s) 1071, 1103
Hsp92II CATG 5 cut(s) 736, 822, 846, 1043, 1120
Kzo9I GATC 1 cut(s) 8
LmnI GCTCC 1 cut(s) 1195
Lsp1109I GCAGC 7 cut(s) 331, 495, 772, 784, 950, 1042, 1145
LweI GCATC 4 cut(s) 464, 673, 882, 1146
MaeI CTAG 2 cut(s) 648, 833
MaeII ACGT 2 cut(s) 1071, 1103
MaeIII GTNAC 3 cut(s) 186, 900, 1085
MalI GATC 1 cut(s) 10
MboI GATC 1 cut(s) 8
MboII GAAGA 8 cut(s) 25, 44, 62, 123, 163, 515, 673, 1243
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 1 cut(s) 1200
MluCI AATT 3 cut(s) 437, 500, 1307
MluI ACGCGT 1 cut(s) 235
MlyI GAGTC 2 cut(s) 368, 865
MmeI TCCRAC 1 cut(s) 516
Mph1103I ATGCAT 2 cut(s) 1041, 1161
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 4 cut(s) 41, 432, 906, 1014
MslI CAYNNNNRTG 1 cut(s) 1034
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 2 cut(s) 14, 114
Mva1269I GAATGC 2 cut(s) 316, 1041
MvnI CGCG 2 cut(s) 237, 273
MwoI GCNNNNNNNGC 5 cut(s) 243, 252, 794, 803, 1097
NcoI CCATGG 1 cut(s) 818
NdeI CATATG 1 cut(s) 1093
NdeII GATC 1 cut(s) 8
NlaIII CATG 5 cut(s) 736, 822, 846, 1043, 1120
NmuCI GTSAC 2 cut(s) 900, 1085
NsiI ATGCAT 2 cut(s) 1041, 1161
PctI GAATGC 2 cut(s) 316, 1041
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 3 cut(s) 100, 259, 969
PflMI CCANNNNNTGG 1 cut(s) 824
PkrI GCNGC 8 cut(s) 254, 346, 485, 787, 799, 940, 1057, 1135
PleI GAGTC 2 cut(s) 367, 865
PmaCI CACGTG 1 cut(s) 1072
PmlI CACGTG 1 cut(s) 1072
PpsI GAGTC 2 cut(s) 367, 865
Ppu21I YACGTR 2 cut(s) 1072, 1104
Psp124BI GAGCTC 1 cut(s) 1200
PspCI CACGTG 1 cut(s) 1072
PstI CTGCAG 2 cut(s) 99, 485
PsuI RGATCY 1 cut(s) 8
PvuII CAGCTG 1 cut(s) 941
RsaI GTAC 2 cut(s) 743, 1106
RsaNI GTAC 2 cut(s) 742, 1105
RseI CAYNNNNRTG 1 cut(s) 1034
SacI GAGCTC 1 cut(s) 1200
SaqAI TTAA 4 cut(s) 41, 432, 906, 1014
SatI GCNGC 8 cut(s) 253, 345, 484, 786, 798, 939, 1056, 1134
Sau3AI GATC 1 cut(s) 8
SchI GAGTC 2 cut(s) 368, 865
SduI GDGCHC 1 cut(s) 1200
SfaNI GCATC 4 cut(s) 464, 673, 882, 1146
SfcI CTRYAG 2 cut(s) 95, 481
SmiMI CAYNNNNRTG 1 cut(s) 1034
SnaBI TACGTA 1 cut(s) 1104
SpeI ACTAGT 1 cut(s) 647
Sse9I AATT 3 cut(s) 437, 500, 1307
SsiI CCGC 2 cut(s) 252, 1193
SspMI CTAG 2 cut(s) 648, 833
SstI GAGCTC 1 cut(s) 1200
StyI CCWWGG 2 cut(s) 654, 818
TaaI ACNGT 5 cut(s) 27, 531, 886, 950, 1170
TaiI ACGT 2 cut(s) 1074, 1106
TaqI TCGA 2 cut(s) 423, 672
TasI AATT 3 cut(s) 437, 500, 1307
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 3 cut(s) 100, 259, 969
Tru1I TTAA 4 cut(s) 41, 432, 906, 1014
Tru9I TTAA 4 cut(s) 41, 432, 906, 1014
TscAI CASTG 5 cut(s) 30, 129, 534, 891, 1173
TseFI GTSAC 2 cut(s) 900, 1085
TseI GCWGC 7 cut(s) 344, 483, 785, 797, 938, 1055, 1133
Tsp45I GTSAC 2 cut(s) 900, 1085
TspDTI ATGAA 4 cut(s) 108, 765, 894, 1241
TspGWI ACGGA 2 cut(s) 93, 967
TspRI CASTG 5 cut(s) 30, 129, 534, 891, 1173
Van91I CCANNNNNTGG 1 cut(s) 824
XapI RAATTY 1 cut(s) 1307
XmiI GTMKAC 2 cut(s) 390, 477
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 648, 833
Zsp2I ATGCAT 2 cut(s) 1041, 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.