FvH4_6g30130

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008507
Physical Location & Seq
Reverse (-)
23278537 .. 23281959
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30130.t1

Sequence Viewer

Length: 1323 bp
ATGGCGGTGATTTCAGTGAAGCCCATGTTCACCGTAGTGGTGGTCACTCTCTCCCTCATCCTTGCCGTCCTTGTCTTCTTTCCCAGTTCGCCTTTCTACCAGTCCCCGCTCTCTCTCGCTTCACCCCAGAGAAAATTGGATATATGGAGTGTTAGGAGGCTGGTGGAGTGGAGACCTTGCAAGTGGTGGCTACAAGGACATCTCACTGCTCTGCCATCAAAGAGTAATGGATTCATTCGAGTGGATTGCTATGGGGGACTTAATCAGATGAGAAGAGATTTATGTGATGGTGTTGGTGTTGCCCGTCTTTTAAATGCAACCCTTGTTCTGCCAAAGTTTGAGGTGGCTGCTTATTGGAATGAATCAAGTGGTTTTGCAGATGTCTTTGATGTCGACTACTTTATTCAACGGATGAATGGCTTCATCACTGTTGTCAAAGAGTTACCATCGGAGATTGGTTCAAAAGAACCCTTCCATGTGGATTGTAGCAAGCGCAAAGGCCAATTTGATTACACAGAAAGTGTTCTTCCATCCCTATTAGAACATCATTATATTTCAATCACGCCTGCTATGAGCCAAAGAAGGGATAGGTATCCTACGTATGCAAAAGCTGCACTTTGCCAAGCATGTTACAGTGCTTTACGCCTCACAAAATCCTTGGAGAAGAAAGCCTCTGAGCTTCTTGATGCCATACCTAAACCTTTTCTCTCACTTCACCTTCGTTTTGAGCCGGATATGGTAGCCTACAGTCAATGTGAATATCCCAACCTAGCTCCTGCTTCTATTGAAGCCATCAAGGCAGCACAGGGAGATAGAAAGCCGTGGACTGGAGAGCTTGCTCAAATTTGGAGGAAGCGAGGAAAATGTCCTCTTACGCCTAATGAGACAGCCTTCATACTACAAGTGCTTTCCATCCCAACAAATACAAACATTTATCTGGCAGCTGGGGACGGTCTGATGGAAATTGAAGGATTAACATCCATTTACACCAATGTAGTTACCAAATCTAGCCTACTTAGTGGTGAGGATTTCAAAAGCATGCATGGAAACACAAAAGCAGCATTGGACTACTACGTGTCTATTAACAGTGATTCATATATAGCAACATATTTCGGAAATATGGATAAGATGGTTGCAGCAATGCGAACTTTTAATGGGTTGTACAAGACTCTTTTCTTAAGCAGAAGAGCTTTTGCTGACCTCACTTCTCAAGGGTTAAGCAGTAATGAATTGGTGCATGCACTGTGGAAGGCTCACAAAGATGATTTTGTCATGGGAAGAGGATCTGCATTGCCTGATTGCTTTTGTGAGTTCAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

441

Amino Acids

49.38

Weight (kDa)

8.66

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 78 - 384 5.1e-63 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 393
AciI CCGC 2 cut(s) 5, 107
AclWI GGATC 1 cut(s) 1291
AcsI RAATTY 1 cut(s) 843
AfaI GTAC 1 cut(s) 1163
AfiI CCNNNNNNNGG 2 cut(s) 583, 827
AflII CTTAAG 1 cut(s) 1177
AflIII ACRYGT 1 cut(s) 1074
AgsI TTSAA 7 cut(s) 407, 462, 558, 788, 968, 1033, 1315
AleI CACNNNNGTG 1 cut(s) 35
AluBI AGCT 6 cut(s) 611, 679, 773, 835, 944, 1190
AluI AGCT 6 cut(s) 611, 679, 773, 835, 944, 1190
Alw26I GTCTC 2 cut(s) 166, 878
AlwI GGATC 1 cut(s) 1291
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 6 cut(s) 347, 611, 800, 941, 1058, 1136
ApoI RAATTY 1 cut(s) 843
Asp700I GAANNNNTTC 2 cut(s) 419, 522
AspLEI GCGC 1 cut(s) 495
AsuHPI GGTGA 5 cut(s) 19, 22, 114, 707, 1034
BbsI GAAGAC 1 cut(s) 67
BbvI GCAGC 6 cut(s) 334, 598, 812, 953, 1070, 1148
BccI CCATC 8 cut(s) 223, 281, 454, 538, 800, 920, 952, 1123
BceAI ACGGC 2 cut(s) 50, 805
BcgI CGANNNNNNTGC 2 cut(s) 228, 262
BciVI GTATCC 1 cut(s) 603
BcoDI GTCTC 2 cut(s) 166, 878
BfaI CTAG 2 cut(s) 770, 1008
BfmI CTRYAG 1 cut(s) 745
BfrI CTTAAG 1 cut(s) 1177
BfuI GTATCC 1 cut(s) 603
BglI GCCNNNNNGGC 1 cut(s) 797
BisI GCNGC 6 cut(s) 348, 612, 801, 942, 1059, 1137
BlsI GCNGC 6 cut(s) 349, 613, 802, 943, 1060, 1138
BmrI ACTGGG 1 cut(s) 78
BmsI GCATC 1 cut(s) 676
BmuI ACTGGG 1 cut(s) 78
BpiI GAAGAC 1 cut(s) 67
BpmI CTGGAG 1 cut(s) 849
BpuEI CTTGAG 1 cut(s) 1194
BsaAI YACGTR 2 cut(s) 600, 1075
BsaBI GATNNNNATC 2 cut(s) 591, 976
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 657, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 583, 827
Bse1I ACTGG 3 cut(s) 84, 100, 832
Bse3DI GCAATG 2 cut(s) 1146, 1289
Bse8I GATNNNNATC 2 cut(s) 591, 976
BseDI CCNNGG 2 cut(s) 657, 821
BseGI GGATG 5 cut(s) 57, 417, 530, 912, 977
BseJI GATNNNNATC 2 cut(s) 591, 976
BseLI CCNNNNNNNGG 2 cut(s) 583, 827
BseMI GCAATG 2 cut(s) 1146, 1289
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 3 cut(s) 84, 100, 832
BseXI GCAGC 6 cut(s) 334, 598, 812, 953, 1070, 1148
BseYI CCCAGC 1 cut(s) 944
BsgI GTGCAG 1 cut(s) 597
BshFI GGCC 1 cut(s) 501
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 3 cut(s) 88, 270, 962
BslI CCNNNNNNNGG 2 cut(s) 583, 827
BsmAI GTCTC 2 cut(s) 166, 878
BsmFI GGGAC 3 cut(s) 88, 270, 962
BsnI GGCC 1 cut(s) 501
Bso31I GGTCTC 1 cut(s) 166
Bsp1407I TGTACA 1 cut(s) 1161
Bsp143I GATC 1 cut(s) 1283
BspACI CCGC 2 cut(s) 5, 107
BspANI GGCC 1 cut(s) 501
BspCNI CTCAG 1 cut(s) 667
BspPI GGATC 1 cut(s) 1291
BspQI GCTCTTC 1 cut(s) 1180
BspTI CTTAAG 1 cut(s) 1177
BspTNI GGTCTC 1 cut(s) 166
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 2 cut(s) 1146, 1289
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 3 cut(s) 84, 100, 832
BssECI CCNNGG 2 cut(s) 657, 821
BssMI GATC 1 cut(s) 1283
BssT1I CCWWGG 1 cut(s) 657
Bst4CI ACNGT 7 cut(s) 34, 430, 635, 749, 953, 1088, 1245
Bst6I CTCTTC 3 cut(s) 268, 1180, 1273
BstAFI CTTAAG 1 cut(s) 1177
BstAPI GCANNNNNTGC 1 cut(s) 611
BstAUI TGTACA 1 cut(s) 1161
BstBAI YACGTR 2 cut(s) 600, 1075
BstC8I GCNNGC 5 cut(s) 491, 567, 837, 1040, 1239
BstDEI CTNAG 2 cut(s) 675, 1016
BstDSI CCRYGG 1 cut(s) 821
BstF5I GGATG 5 cut(s) 57, 417, 530, 912, 977
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 1 cut(s) 1286
BstMAI GTCTC 2 cut(s) 166, 878
BstMBI GATC 1 cut(s) 1283
BstMWI GCNNNNNNNGC 2 cut(s) 611, 797
BstNSI RCATGY 3 cut(s) 630, 1042, 1241
BstSFI CTRYAG 1 cut(s) 745
BstSNI TACGTA 1 cut(s) 600
BstV1I GCAGC 6 cut(s) 334, 598, 812, 953, 1070, 1148
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 1283
BstYI RGATCY 1 cut(s) 1283
BsuI GTATCC 1 cut(s) 603
BsuRI GGCC 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 821
BtsCI GGATG 5 cut(s) 57, 417, 530, 912, 977
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 6 cut(s) 21, 204, 426, 640, 1093, 1241
Cac8I GCNNGC 5 cut(s) 491, 567, 837, 1040, 1239
CfoI GCGC 1 cut(s) 495
Csp6I GTAC 1 cut(s) 1162
CviAII CATG 7 cut(s) 25, 476, 627, 1039, 1043, 1238, 1273
CviQI GTAC 1 cut(s) 1162
DdeI CTNAG 2 cut(s) 675, 1016
DpnI GATC 1 cut(s) 1285
DpnII GATC 1 cut(s) 1283
DraI TTTAAA 1 cut(s) 312
Eam1104I CTCTTC 3 cut(s) 268, 1180, 1273
EarI CTCTTC 3 cut(s) 268, 1180, 1273
Eco105I TACGTA 1 cut(s) 600
Eco130I CCWWGG 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 657
EcoT22I ATGCAT 1 cut(s) 1044
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 7 cut(s) 28, 479, 630, 1042, 1046, 1241, 1276
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 3 cut(s) 88, 270, 962
FatI CATG 7 cut(s) 24, 475, 626, 1038, 1042, 1237, 1272
FauI CCCGC 1 cut(s) 114
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 6 cut(s) 348, 612, 801, 942, 1059, 1137
FokI GGATG 5 cut(s) 44, 424, 517, 899, 964
Fsp4HI GCNGC 6 cut(s) 348, 612, 801, 942, 1059, 1137
FspBI CTAG 2 cut(s) 770, 1008
GlaI GCGC 1 cut(s) 494
GluI GCNGC 6 cut(s) 348, 612, 801, 942, 1059, 1137
GsaI CCCAGC 1 cut(s) 948
GsuI CTGGAG 1 cut(s) 849
HaeIII GGCC 1 cut(s) 501
HapII CCGG 1 cut(s) 731
HhaI GCGC 1 cut(s) 495
Hin1II CATG 7 cut(s) 28, 479, 630, 1042, 1046, 1241, 1276
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 4 cut(s) 231, 362, 1091, 1168
HpaII CCGG 1 cut(s) 731
HphI GGTGA 5 cut(s) 19, 22, 114, 707, 1034
Hpy166II GTNNAC 3 cut(s) 30, 394, 825
Hpy188I TCNGA 5 cut(s) 267, 451, 676, 957, 1115
Hpy188III TCNNGA 1 cut(s) 683
Hpy8I GTNNAC 3 cut(s) 30, 394, 825
HpyAV CCTTC 6 cut(s) 481, 576, 728, 901, 962, 1243
HpyCH4III ACNGT 7 cut(s) 34, 430, 635, 749, 953, 1088, 1245
HpyCH4IV ACGT 2 cut(s) 599, 1074
HpyF10VI GCNNNNNNNGC 2 cut(s) 611, 797
HpyF3I CTNAG 2 cut(s) 675, 1016
HpySE526I ACGT 2 cut(s) 599, 1074
Hsp92II CATG 7 cut(s) 28, 479, 630, 1042, 1046, 1241, 1276
HspAI GCGC 1 cut(s) 493
Kzo9I GATC 1 cut(s) 1283
LguI GCTCTTC 1 cut(s) 1180
LmnI GCTCC 1 cut(s) 778
Lsp1109I GCAGC 6 cut(s) 334, 598, 812, 953, 1070, 1148
LweI GCATC 1 cut(s) 676
MaeI CTAG 2 cut(s) 770, 1008
MaeII ACGT 2 cut(s) 599, 1074
MaeIII GTNAC 4 cut(s) 43, 441, 629, 997
MalI GATC 1 cut(s) 1285
MbiI CCGCTC 1 cut(s) 109
MboI GATC 1 cut(s) 1283
MboII GAAGA 6 cut(s) 67, 285, 518, 676, 1197, 1290
MflI RGATCY 1 cut(s) 1283
MluCI AATT 6 cut(s) 134, 503, 843, 963, 1229, 1316
MlyI GAGTC 1 cut(s) 1162
Mph1103I ATGCAT 1 cut(s) 1044
MroXI GAANNNNTTC 2 cut(s) 419, 522
MseI TTAA 7 cut(s) 261, 311, 974, 1083, 1152, 1178, 1217
MslI CAYNNNNRTG 3 cut(s) 35, 239, 1260
MspA1I CMGCKG 1 cut(s) 944
MspCI CTTAAG 1 cut(s) 1177
MspI CCGG 1 cut(s) 731
MwoI GCNNNNNNNGC 2 cut(s) 611, 797
NdeII GATC 1 cut(s) 1283
NlaIII CATG 7 cut(s) 28, 479, 630, 1042, 1046, 1241, 1276
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 1044
NspI RCATGY 3 cut(s) 630, 1042, 1241
OliI CACNNNNGTG 1 cut(s) 35
PaeI GCATGC 2 cut(s) 1042, 1241
PciSI GCTCTTC 1 cut(s) 1180
PdmI GAANNNNTTC 2 cut(s) 419, 522
PfeI GAWTC 3 cut(s) 231, 362, 1091
PkrI GCNGC 6 cut(s) 349, 613, 802, 943, 1060, 1138
PleI GAGTC 1 cut(s) 1162
PpsI GAGTC 1 cut(s) 1162
Ppu21I YACGTR 2 cut(s) 600, 1075
PspFI CCCAGC 1 cut(s) 944
PsuI RGATCY 1 cut(s) 1283
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 1 cut(s) 1163
RsaNI GTAC 1 cut(s) 1162
RseI CAYNNNNRTG 3 cut(s) 35, 239, 1260
SalI GTCGAC 1 cut(s) 392
SapI GCTCTTC 1 cut(s) 1180
SaqAI TTAA 7 cut(s) 261, 311, 974, 1083, 1152, 1178, 1217
SatI GCNGC 6 cut(s) 348, 612, 801, 942, 1059, 1137
Sau3AI GATC 1 cut(s) 1283
SchI GAGTC 1 cut(s) 1162
SfaNI GCATC 1 cut(s) 676
SfcI CTRYAG 1 cut(s) 745
SmiMI CAYNNNNRTG 3 cut(s) 35, 239, 1260
SmlI CTYRAG 2 cut(s) 1177, 1209
SmoI CTYRAG 2 cut(s) 1177, 1209
SnaBI TACGTA 1 cut(s) 600
SphI GCATGC 2 cut(s) 1042, 1241
Sse9I AATT 6 cut(s) 134, 503, 843, 963, 1229, 1316
SsiI CCGC 2 cut(s) 5, 107
SspMI CTAG 2 cut(s) 770, 1008
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 7 cut(s) 34, 430, 635, 749, 953, 1088, 1245
TaiI ACGT 2 cut(s) 602, 1077
TaqI TCGA 2 cut(s) 238, 393
TasI AATT 6 cut(s) 134, 503, 843, 963, 1229, 1316
TatI WGTACW 1 cut(s) 1161
TfiI GAWTC 3 cut(s) 231, 362, 1091
Tru1I TTAA 7 cut(s) 261, 311, 974, 1083, 1152, 1178, 1217
Tru9I TTAA 7 cut(s) 261, 311, 974, 1083, 1152, 1178, 1217
TscAI CASTG 6 cut(s) 21, 211, 433, 640, 1093, 1248
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 6 cut(s) 347, 611, 800, 941, 1058, 1136
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 7 cut(s) 223, 375, 412, 428, 883, 1083, 1242
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 6 cut(s) 21, 211, 433, 640, 1093, 1248
Vha464I CTTAAG 1 cut(s) 1177
XapI RAATTY 1 cut(s) 843
XceI RCATGY 3 cut(s) 630, 1042, 1241
XmiI GTMKAC 1 cut(s) 393
XmnI GAANNNNTTC 2 cut(s) 419, 522
XspI CTAG 2 cut(s) 770, 1008
Zsp2I ATGCAT 1 cut(s) 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.