RchiOBHm_Chr2g0136261

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008507
Physical Location & Seq
Reverse (-)
53627513 .. 53629875
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50708

Sequence Viewer

Length: 906 bp
ATGGCGGTGATTTCAGTGAAGCCCATGTTCACTATAGTGGTGGTCACTCTCTCTCTCATCCTTGCCGTCCTTGTCTTCTCATCCCCCACTTCGCCTTTCTACCAATCCCCCCTCTCTCTTGCTTCACCCCAAAGAAAATTGGATACATGGAGTGTTGGGAGGTTGGTAGAGTGGAGACCTTGCAAGTGGTGGCTACAAGGACATCGCACCGCTCTGCCATCAAAGAGTAATGGATTCATTCGAGTGGATTGCTATGGTCGCCTTAATCAGATGAGAAGAGATTTATGTGATGGTGTTGGTGTTGCCCGTCTTTTAAATGCAACCCTTGTTTTGCCAAAGTTTGAGGTGGCTGCTTATTGGAATGAATCAAGTGGTTTTGCAGATATCTTTGATGTCGACTACTTCGTTCAACGGATGAATGGCTTCATCAGTGTTGTCAAAGAGTTACCATCTGAGATTGCATCAAAAGAACCCTTCCATGTGGATTGTAGCAAACGCAACGGCCAATTTGATTACATAGAAAGTGTTCTTCCATCCCTATTAGAACATCATTATATTTCAATCACGCCAGCAATGAGCCAAAGAAGGGATAGGTATCCTCAGTATGCAAAAGCTGCCCTTTGCCAAGCATGTTACAGTGCTTTACGCCTCACAAAATCCCTGAAGAAGAAAGCCTCGGAGCTTCTTGATGCCATACCTAAACCTTTTCTCTCACTTCACCTTCGTTTTGAGCCTGATATGGTAGCTTACAGTCAATGTGAATATCCATACCTAGCTCCTGCTTCTATTGAAGCCATCAAGGCAGCACAGGGAGATAGAAAGCCATGGACCGGAGAGCTTGCCCAAATTTGGAGGAAACGAGGAAAATGTCCTCTTACGCCTAATGAGACAGCCTTCATACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

301

Amino Acids

34.1

Weight (kDa)

9.2

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 79 - 296 1.8e-41 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 212
AccI GTMKAC 1 cut(s) 396
AciI CCGC 2 cut(s) 5, 210
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 1 cut(s) 846
AcuI CTGAAG 1 cut(s) 683
AfiI CCNNNNNNNGG 3 cut(s) 586, 830, 849
AgsI TTSAA 3 cut(s) 410, 561, 791
AleI CACNNNNGTG 1 cut(s) 35
AluBI AGCT 5 cut(s) 614, 682, 746, 776, 838
AluI AGCT 5 cut(s) 614, 682, 746, 776, 838
Alw26I GTCTC 2 cut(s) 169, 881
AoxI GGCC 1 cut(s) 502
ApeKI GCWGC 3 cut(s) 350, 614, 803
ApoI RAATTY 1 cut(s) 846
Asp700I GAANNNNTTC 2 cut(s) 422, 525
AspS9I GGNCC 1 cut(s) 828
AsuHPI GGTGA 3 cut(s) 19, 117, 710
AvaII GGWCC 1 cut(s) 828
BbsI GAAGAC 1 cut(s) 67
BbvI GCAGC 3 cut(s) 337, 601, 815
BccI CCATC 5 cut(s) 226, 284, 457, 541, 803
BceAI ACGGC 2 cut(s) 50, 517
BcgI CGANNNNNNTGC 2 cut(s) 231, 265
BciVI GTATCC 2 cut(s) 136, 606
BcoDI GTCTC 2 cut(s) 169, 881
BfaI CTAG 1 cut(s) 773
BfmI CTRYAG 1 cut(s) 33
BfuI GTATCC 2 cut(s) 136, 606
BglI GCCNNNNNGGC 1 cut(s) 800
BisI GCNGC 3 cut(s) 351, 615, 804
BlsI GCNGC 3 cut(s) 352, 616, 805
Bme18I GGWCC 1 cut(s) 828
BmgT120I GGNCC 1 cut(s) 828
BmsI GCATC 2 cut(s) 470, 679
BpiI GAAGAC 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 2 cut(s) 675, 824
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 3 cut(s) 586, 830, 849
Bse3DI GCAATG 1 cut(s) 579
Bse8I GATNNNNATC 1 cut(s) 594
BseDI CCNNGG 2 cut(s) 675, 824
BseGI GGATG 4 cut(s) 57, 80, 420, 533
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 3 cut(s) 586, 830, 849
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 2 cut(s) 444, 614
BseXI GCAGC 3 cut(s) 337, 601, 815
BshFI GGCC 1 cut(s) 504
BsiSI CCGG 1 cut(s) 831
BslI CCNNNNNNNGG 3 cut(s) 586, 830, 849
BsmAI GTCTC 2 cut(s) 169, 881
BsnI GGCC 1 cut(s) 504
Bso31I GGTCTC 1 cut(s) 169
Bsp19I CCATGG 1 cut(s) 824
BspACI CCGC 2 cut(s) 5, 210
BspANI GGCC 1 cut(s) 504
BspCNI CTCAG 2 cut(s) 445, 613
BspTNI GGTCTC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 579
BssECI CCNNGG 2 cut(s) 675, 824
BssT1I CCWWGG 1 cut(s) 824
Bst4CI ACNGT 2 cut(s) 638, 752
Bst6I CTCTTC 1 cut(s) 271
BstAPI GCANNNNNTGC 1 cut(s) 614
BstC8I GCNNGC 2 cut(s) 570, 840
BstDEI CTNAG 2 cut(s) 453, 600
BstDSI CCRYGG 1 cut(s) 824
BstF5I GGATG 4 cut(s) 57, 80, 420, 533
BstMAI GTCTC 2 cut(s) 169, 881
BstMWI GCNNNNNNNGC 3 cut(s) 258, 614, 800
BstNSI RCATGY 1 cut(s) 633
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 337, 601, 815
BstV2I GAAGAC 1 cut(s) 67
BsuI GTATCC 2 cut(s) 136, 606
BsuRI GGCC 1 cut(s) 504
BtgI CCRYGG 1 cut(s) 824
BtgZI GCGATG 1 cut(s) 188
BtsCI GGATG 4 cut(s) 57, 80, 420, 533
BtsIMutI CAGTG 3 cut(s) 21, 436, 643
Cac8I GCNNGC 2 cut(s) 570, 840
Cfr13I GGNCC 1 cut(s) 828
CviAII CATG 5 cut(s) 25, 147, 479, 630, 825
DdeI CTNAG 2 cut(s) 453, 600
DraI TTTAAA 1 cut(s) 315
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 824
Eco31I GGTCTC 1 cut(s) 169
Eco32I GATATC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 828
Eco57I CTGAAG 1 cut(s) 683
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 824
ErhI CCWWGG 1 cut(s) 824
FaeI CATG 5 cut(s) 28, 150, 482, 633, 828
FalI AAGNNNNNCTT 2 cut(s) 603, 635
FatI CATG 5 cut(s) 24, 146, 478, 629, 824
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 3 cut(s) 351, 615, 804
FokI GGATG 4 cut(s) 44, 67, 427, 520
Fsp4HI GCNGC 3 cut(s) 351, 615, 804
FspBI CTAG 1 cut(s) 773
GluI GCNGC 3 cut(s) 351, 615, 804
HaeIII GGCC 1 cut(s) 504
HapII CCGG 1 cut(s) 831
Hin1II CATG 5 cut(s) 28, 150, 482, 633, 828
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 2 cut(s) 234, 365
HpaII CCGG 1 cut(s) 831
HphI GGTGA 3 cut(s) 19, 117, 710
Hpy166II GTNNAC 2 cut(s) 30, 397
Hpy188I TCNGA 3 cut(s) 270, 454, 679
Hpy188III TCNNGA 1 cut(s) 686
Hpy8I GTNNAC 2 cut(s) 30, 397
HpyAV CCTTC 4 cut(s) 484, 579, 731, 904
HpyCH4III ACNGT 2 cut(s) 638, 752
HpyCH4V TGCA 5 cut(s) 183, 320, 380, 461, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 614, 800
HpyF3I CTNAG 2 cut(s) 453, 600
Hsp92II CATG 5 cut(s) 28, 150, 482, 633, 828
LmnI GCTCC 2 cut(s) 679, 781
LpnPI CCDG 6 cut(s) 582, 674, 747, 792, 794, 844
Lsp1109I GCAGC 3 cut(s) 337, 601, 815
LweI GCATC 2 cut(s) 470, 679
MaeI CTAG 1 cut(s) 773
MaeIII GTNAC 3 cut(s) 43, 444, 632
MbiI CCGCTC 1 cut(s) 212
MboII GAAGA 5 cut(s) 67, 288, 521, 676, 679
MluCI AATT 3 cut(s) 137, 506, 846
MnlI CCTC 9 cut(s) 122, 153, 337, 609, 659, 685, 846, 854, 882
MroXI GAANNNNTTC 2 cut(s) 422, 525
MseI TTAA 3 cut(s) 264, 314, 904
MslI CAYNNNNRTG 2 cut(s) 35, 242
MspI CCGG 1 cut(s) 831
MwoI GCNNNNNNNGC 3 cut(s) 258, 614, 800
NcoI CCATGG 1 cut(s) 824
NlaIII CATG 5 cut(s) 28, 150, 482, 633, 828
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 633
OliI CACNNNNGTG 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PdmI GAANNNNTTC 2 cut(s) 422, 525
PfeI GAWTC 2 cut(s) 234, 365
PkrI GCNGC 3 cut(s) 352, 616, 805
PspPI GGNCC 1 cut(s) 828
RseI CAYNNNNRTG 2 cut(s) 35, 242
SalI GTCGAC 1 cut(s) 395
SaqAI TTAA 3 cut(s) 264, 314, 904
SatI GCNGC 3 cut(s) 351, 615, 804
Sau96I GGNCC 1 cut(s) 828
SfaNI GCATC 2 cut(s) 470, 679
SfcI CTRYAG 1 cut(s) 33
SinI GGWCC 1 cut(s) 828
SmiMI CAYNNNNRTG 2 cut(s) 35, 242
Sse9I AATT 3 cut(s) 137, 506, 846
SsiI CCGC 2 cut(s) 5, 210
SspMI CTAG 1 cut(s) 773
StyI CCWWGG 1 cut(s) 824
TaaI ACNGT 2 cut(s) 638, 752
TaqI TCGA 2 cut(s) 241, 396
TasI AATT 3 cut(s) 137, 506, 846
TfiI GAWTC 2 cut(s) 234, 365
Tru1I TTAA 3 cut(s) 264, 314, 904
Tru9I TTAA 3 cut(s) 264, 314, 904
TscAI CASTG 3 cut(s) 21, 436, 643
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 3 cut(s) 350, 614, 803
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 5 cut(s) 226, 378, 415, 431, 886
TspGWI ACGGA 1 cut(s) 427
TspRI CASTG 3 cut(s) 21, 436, 643
VpaK11BI GGWCC 1 cut(s) 828
XapI RAATTY 1 cut(s) 846
XceI RCATGY 1 cut(s) 633
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 2 cut(s) 422, 525
XspI CTAG 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.