pycom09g13880

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008507
Physical Location & Seq
Forward (+)
13047016 .. 13048163
1148 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g13880.1

Sequence Viewer

Length: 645 bp
ATGCAGTATAGTGCAGTGTGGGGTTTGGGGGCTAGGTATGCAAAAGCTTCCCTTTGTCAAGCTTGCTACAGTGCATTATGCCTCACCAAATCCTTGGAGAAGAAAGCTTCTAAGCTTCTTGAAGCCATACCCAAACCATTCCTCTCTTTGCATCTTCGTTTTGAGCCTGATATGGTAGCTTACAGTCAATGTGAATACCCCTACCTTTCTCCTTCCTTCATTGAAGCCATTACGGCTGTGCGAGGAGATAGAAAACCATGGACTAGAGATGTTTTCCATCCCAACGAACACAAACATTTATCTGGCTGCTGGGGACGGTCTGATGGAATTGAAGGGTTAACTTTTGTTTACACAAATGTAGTTACCAAGTCTAGCCTTCTCAGTGGCAAGGATTTCAAAAGCATGCATGGGAACACGAAAGCGGCCTTGGATTACTACGTGTCTATTAACAACGATTCATATATAGCAACGTATGTTGGAAATATGGATAAGATGGTTGCAGCAAAGCGAGCTTTTAACAAGTTCACATGTCAAGGTTTAAGGGGGAAAGAATTGATGCGAGCTCTATGGAAGGCGCATAAAGATGATTTTGTCATGGGGAAAGGATCTGCTTTGCCCAATTGCTTTTGTGAGTTCAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

215

Amino Acids

24.07

Weight (kDa)

9.19

Isoelectric Point (pI)

26.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 24 - 81 7.6e-07 GDP-fucose protein O-fucosyltransferase
O-FucT PF10250 110 - 172 1.9e-07 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 422
AclWI GGATC 1 cut(s) 613
AflIII ACRYGT 2 cut(s) 438, 527
AgsI TTSAA 5 cut(s) 122, 224, 332, 397, 637
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AluBI AGCT 7 cut(s) 47, 62, 107, 115, 179, 512, 563
AluI AGCT 7 cut(s) 47, 62, 107, 115, 179, 512, 563
Alw21I GWGCWC 1 cut(s) 565
AlwI GGATC 1 cut(s) 613
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 2 cut(s) 306, 500
AspLEI GCGC 1 cut(s) 577
AsuHPI GGTGA 1 cut(s) 76
BanII GRGCYC 1 cut(s) 565
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 2 cut(s) 293, 512
BccI CCATC 3 cut(s) 285, 317, 487
BceAI ACGGC 1 cut(s) 249
BfaI CTAG 3 cut(s) 33, 264, 372
BfmI CTRYAG 1 cut(s) 67
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 3 cut(s) 307, 423, 501
BlsI GCNGC 3 cut(s) 308, 424, 502
BmsI GCATC 2 cut(s) 160, 546
BsaAI YACGTR 1 cut(s) 439
BsaJI CCNNGG 3 cut(s) 93, 257, 426
BseDI CCNNGG 3 cut(s) 93, 257, 426
BseGI GGATG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 258
BseXI GCAGC 2 cut(s) 293, 512
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 565
BslFI GGGAC 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 327
BsnI GGCC 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 565
Bsp143I GATC 1 cut(s) 605
Bsp19I CCATGG 1 cut(s) 257
BspACI CCGC 1 cut(s) 422
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 1 cut(s) 393
BspPI GGATC 1 cut(s) 613
BssECI CCNNGG 3 cut(s) 93, 257, 426
BssMI GATC 1 cut(s) 605
BssT1I CCWWGG 3 cut(s) 93, 257, 426
Bst4CI ACNGT 3 cut(s) 71, 185, 318
BstBAI YACGTR 1 cut(s) 439
BstC8I GCNNGC 4 cut(s) 64, 404, 510, 561
BstDEI CTNAG 2 cut(s) 111, 380
BstDSI CCRYGG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 277
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 1 cut(s) 608
BstMBI GATC 1 cut(s) 605
BstMWI GCNNNNNNNGC 3 cut(s) 38, 233, 509
BstNSI RCATGY 2 cut(s) 406, 531
BstSFI CTRYAG 1 cut(s) 67
BstV1I GCAGC 2 cut(s) 293, 512
BstX2I RGATCY 1 cut(s) 605
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 605
BsuRI GGCC 1 cut(s) 425
BtgI CCRYGG 1 cut(s) 257
BtsCI GGATG 1 cut(s) 277
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 3 cut(s) 21, 76, 388
Cac8I GCNNGC 4 cut(s) 64, 404, 510, 561
CfoI GCGC 1 cut(s) 577
CviAII CATG 5 cut(s) 258, 403, 407, 528, 595
DdeI CTNAG 2 cut(s) 111, 380
DpnI GATC 1 cut(s) 607
DpnII GATC 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 563
Eco130I CCWWGG 3 cut(s) 93, 257, 426
Eco24I GRGCYC 1 cut(s) 565
Eco53kI GAGCTC 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 563
EcoT14I CCWWGG 3 cut(s) 93, 257, 426
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 1 cut(s) 565
ErhI CCWWGG 3 cut(s) 93, 257, 426
FaeI CATG 5 cut(s) 261, 406, 410, 531, 598
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 1 cut(s) 327
FatI CATG 5 cut(s) 257, 402, 406, 527, 594
Fnu4HI GCNGC 3 cut(s) 307, 423, 501
FokI GGATG 1 cut(s) 264
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 3 cut(s) 307, 423, 501
FspBI CTAG 3 cut(s) 33, 264, 372
GlaI GCGC 1 cut(s) 576
GluI GCNGC 3 cut(s) 307, 423, 501
GsaI CCCAGC 1 cut(s) 313
HaeIII GGCC 1 cut(s) 425
HhaI GCGC 1 cut(s) 577
Hin1II CATG 5 cut(s) 261, 406, 410, 531, 598
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HindIII AAGCTT 4 cut(s) 45, 60, 105, 113
HinfI GANTC 1 cut(s) 455
HpaI GTTAAC 1 cut(s) 339
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 3 cut(s) 339, 349, 525
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 3 cut(s) 339, 349, 525
HpyAV CCTTC 5 cut(s) 222, 226, 326, 386, 565
HpyCH4III ACNGT 3 cut(s) 71, 185, 318
HpyCH4IV ACGT 2 cut(s) 438, 470
HpyCH4V TGCA 7 cut(s) 4, 14, 41, 74, 151, 406, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 233, 509
HpyF3I CTNAG 2 cut(s) 111, 380
HpySE526I ACGT 2 cut(s) 438, 470
Hsp92II CATG 5 cut(s) 261, 406, 410, 531, 598
HspAI GCGC 1 cut(s) 575
KspAI GTTAAC 1 cut(s) 339
Kzo9I GATC 1 cut(s) 605
LpnPI CCDG 3 cut(s) 180, 288, 295
Lsp1109I GCAGC 2 cut(s) 293, 512
LweI GCATC 2 cut(s) 160, 546
MaeI CTAG 3 cut(s) 33, 264, 372
MaeII ACGT 2 cut(s) 438, 470
MaeIII GTNAC 1 cut(s) 361
MalI GATC 1 cut(s) 607
MboI GATC 1 cut(s) 605
MboII GAAGA 2 cut(s) 112, 146
MfeI CAATTG 1 cut(s) 619
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 1 cut(s) 565
MluCI AATT 4 cut(s) 327, 551, 619, 638
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 3 cut(s) 92, 152, 236
Mph1103I ATGCAT 1 cut(s) 408
MseI TTAA 4 cut(s) 338, 447, 516, 539
MslI CAYNNNNRTG 1 cut(s) 582
MunI CAATTG 1 cut(s) 619
MwoI GCNNNNNNNGC 3 cut(s) 38, 233, 509
NcoI CCATGG 1 cut(s) 257
NdeII GATC 1 cut(s) 605
NlaIII CATG 5 cut(s) 261, 406, 410, 531, 598
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 2 cut(s) 406, 531
PaeI GCATGC 1 cut(s) 406
PciI ACATGT 1 cut(s) 527
PfeI GAWTC 1 cut(s) 455
PkrI GCNGC 3 cut(s) 308, 424, 502
Ppu21I YACGTR 1 cut(s) 439
PscI ACATGT 1 cut(s) 527
Psp124BI GAGCTC 1 cut(s) 565
PspFI CCCAGC 1 cut(s) 309
PsuI RGATCY 1 cut(s) 605
RseI CAYNNNNRTG 1 cut(s) 582
SacI GAGCTC 1 cut(s) 565
SaqAI TTAA 4 cut(s) 338, 447, 516, 539
SatI GCNGC 3 cut(s) 307, 423, 501
Sau3AI GATC 1 cut(s) 605
SduI GDGCHC 1 cut(s) 565
SfaNI GCATC 2 cut(s) 160, 546
SfcI CTRYAG 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 582
SphI GCATGC 1 cut(s) 406
Sse9I AATT 4 cut(s) 327, 551, 619, 638
SsiI CCGC 1 cut(s) 422
SspMI CTAG 3 cut(s) 33, 264, 372
SstI GAGCTC 1 cut(s) 565
StyI CCWWGG 3 cut(s) 93, 257, 426
TaaI ACNGT 3 cut(s) 71, 185, 318
TaiI ACGT 2 cut(s) 441, 473
TasI AATT 4 cut(s) 327, 551, 619, 638
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 1 cut(s) 455
Tru1I TTAA 4 cut(s) 338, 447, 516, 539
Tru9I TTAA 4 cut(s) 338, 447, 516, 539
TscAI CASTG 3 cut(s) 21, 76, 388
TseI GCWGC 2 cut(s) 306, 500
TspDTI ATGAA 2 cut(s) 208, 447
TspRI CASTG 3 cut(s) 21, 76, 388
XceI RCATGY 2 cut(s) 406, 531
XspI CTAG 3 cut(s) 33, 264, 372
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.