Rroxscaffold_2G00108420

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008507
Physical Location & Seq
Forward (+)
32411524 .. 32425217
13694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108420.1

Sequence Viewer

Length: 1383 bp
ATGTTGAGAAACCGGCTTTTGGGTTTGGGGCTACTTGATGACATTGAAATACTTGCTATTAAGATGGAGCAAAGCACTTCTTGCTCAATACAAGAGTTCGCAAAAGTGCTGAGAGGGAACAAAGCCTCTTTTCACCACTATATTGATATGGATCACCCTATGACAGTGTCACGTGATAATGCAAATAGTACATCTTATTGTTTGGCCGTCGAAGCTCTGCCATCAAAGAGTAATGGATTCATTCGAGTGGATTGCTATGGTGGCCTTAATCAGATGAGAAGAGATTTGTGTGATGGGGTTGGTATTGCCCGTCTTTTAAATGCAACCCTTGTTCTGCCAAAGTTTGAGGTGGCTGCTTATTGGAATGAATCAAGTGGTTTTGCAGACGTCTTTGATGTTGACTACTTCATTCAACAGTTGAATGGCTTTATCAGAGTTGTCAAAGAGTTACCACACGAGTTTGCATCAAAAGAACCCTTCCGTGTAGATTGCAGCAAACGTAAAGGCCAATTTGATTATATTGAAAATGTTCTTCCGTCTTTATTAGAACATCATTATATATCCATCACACCAGCAATGAGCCAAAGAAGGGACAGGTATCCTCAGTTTGCAAAAGCTGCCCTTTGTCAAGCATGTTACAGTGCATTACGCCTCACGAAAGCCCTGGAGAAGAAGGCTTCCGAGCTTCTTGAAGCCATTCCTAAACCTTTTCTGTCACTTCACCTTCGTTTTGAGCCTGATATGGTAGCCTACAGTCAATGTGAATACCCCTACCTTTCTCCTGCCTCCATTGAAGCTGTTAAGGCAGCACAAGGTGATAGAAAACCTTGGACTGGGGAGCTTGCCCAAAGTTGGAGGAAACGAGGGAAATGTCCTCTTACACCTAATGAGACAGCATTCATACTCCAAGTGCTTTCCATCCCAACCAACACAAACATTTATCTTGCAGCTGGGGATGGCCTGATGGAAATTGAAGGATTAACATCTGTTTACACAAATGTAGTTACCAAGTCTAGCTTACTTAGTGGGGAGGATTTCAAAAGCATGCATGGGAACACCAAAGCTGCTTTGGATTATTATGTGTCTATTAACAGTGATTCATATATAGCAACATATTTTGGAAATATGGATAAGATGGTTGCAGCAATGCGAGCTTATAACGGCTTGTACAAGACTCTTTTCTTAAGCAGAAGAGCTTTTGCAGAGTACACGTCTCAAGGTTTGATAGGGAAGGAATTGATGCAAGCCCTATGGAAGACTCATAGAGATGATTTTGATGCCATACATACTCGAGCCCTCCGTCCAGCTTCACTTTCCTGCAAGTCAATTATAGCCCTTTTAAAAAAACCTCTAAGACTCATGGTTGATTGTGATATTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

460

Amino Acids

51.76

Weight (kDa)

8.35

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 80 - 387 1.3e-63 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1158
AatII GACGTC 1 cut(s) 390
AclWI GGATC 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 204
AcvI CACGTG 1 cut(s) 173
AcyI GRCGYC 1 cut(s) 387
AdeI CACNNNGTG 1 cut(s) 815
AfaI GTAC 3 cut(s) 190, 1169, 1208
AfiI CCNNNNNNNGG 4 cut(s) 19, 589, 833, 852
AflII CTTAAG 1 cut(s) 1183
AflIII ACRYGT 1 cut(s) 1209
AgsI TTSAA 8 cut(s) 47, 413, 421, 524, 692, 794, 974, 1039
AjiI CACGTC 1 cut(s) 1212
AjnI CCWGG 1 cut(s) 663
Alw26I GTCTC 2 cut(s) 884, 1218
AlwI GGATC 1 cut(s) 159
Ama87I CYCGRG 1 cut(s) 1290
AoxI GGCC 4 cut(s) 204, 262, 505, 958
ApeKI GCWGC 7 cut(s) 353, 492, 617, 806, 947, 1064, 1142
Asp700I GAANNNNTTC 2 cut(s) 528, 696
AsuHPI GGTGA 4 cut(s) 125, 146, 713, 827
AvaI CYCGRG 1 cut(s) 1290
BanII GRGCYC 1 cut(s) 1297
BauI CACGAG 1 cut(s) 455
BbrPI CACGTG 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 1262
BbvI GCAGC 7 cut(s) 340, 504, 604, 818, 959, 1051, 1154
BccI CCATC 8 cut(s) 58, 229, 287, 572, 926, 950, 958, 1129
BceAI ACGGC 2 cut(s) 191, 1177
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 1 cut(s) 665
BciVI GTATCC 1 cut(s) 609
BcoDI GTCTC 2 cut(s) 884, 1218
BfaI CTAG 1 cut(s) 1014
BfmI CTRYAG 1 cut(s) 751
BfrI CTTAAG 1 cut(s) 1183
BfuI GTATCC 1 cut(s) 609
BisI GCNGC 7 cut(s) 354, 493, 618, 807, 948, 1065, 1143
BlsI GCNGC 7 cut(s) 355, 494, 619, 808, 949, 1066, 1144
Bme1390I CCNGG 1 cut(s) 665
BmeT110I CYCGRG 1 cut(s) 1290
BmgBI CACGTC 1 cut(s) 1212
BmrFI CCNGG 1 cut(s) 665
BmrI ACTGGG 1 cut(s) 843
BmsI GCATC 3 cut(s) 473, 1230, 1267
BmuI ACTGGG 1 cut(s) 843
BpiI GAAGAC 1 cut(s) 1262
BpmI CTGGAG 1 cut(s) 686
BpuEI CTTGAG 1 cut(s) 1200
BsaAI YACGTR 1 cut(s) 173
BsaBI GATNNNNATC 2 cut(s) 150, 982
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 2 cut(s) 663, 827
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 589, 833, 852
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 1 cut(s) 838
Bse3DI GCAATG 2 cut(s) 582, 1152
Bse8I GATNNNNATC 2 cut(s) 150, 982
BseBI CCWGG 1 cut(s) 665
BseDI CCNNGG 2 cut(s) 663, 827
BseGI GGATG 2 cut(s) 918, 961
BseJI GATNNNNATC 2 cut(s) 150, 982
BseLI CCNNNNNNNGG 4 cut(s) 19, 589, 833, 852
BseMI GCAATG 2 cut(s) 582, 1152
BseMII CTCAG 2 cut(s) 101, 617
BseNI ACTGG 1 cut(s) 838
BseXI GCAGC 7 cut(s) 340, 504, 604, 818, 959, 1051, 1154
BseYI CCCAGC 1 cut(s) 950
BshFI GGCC 4 cut(s) 206, 264, 507, 960
BsiHKCI CYCGRG 1 cut(s) 1290
BsiSI CCGG 1 cut(s) 13
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 4 cut(s) 19, 589, 833, 852
BsmAI GTCTC 2 cut(s) 884, 1218
BsmBI CGTCTC 1 cut(s) 1218
BsmFI GGGAC 1 cut(s) 605
BsmI GAATGC 1 cut(s) 896
BsnI GGCC 4 cut(s) 206, 264, 507, 960
BsoBI CYCGRG 1 cut(s) 1290
Bsp1286I GDGCHC 1 cut(s) 1297
Bsp1407I TGTACA 1 cut(s) 1167
Bsp143I GATC 1 cut(s) 151
BspANI GGCC 4 cut(s) 206, 264, 507, 960
BspCNI CTCAG 2 cut(s) 102, 616
BspPI GGATC 1 cut(s) 159
BspQI GCTCTTC 1 cut(s) 1186
BspTI CTTAAG 1 cut(s) 1183
BsrDI GCAATG 2 cut(s) 582, 1152
BsrFI RCCGGY 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 1167
BsrI ACTGG 1 cut(s) 838
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 2 cut(s) 663, 827
BssMI GATC 1 cut(s) 151
BssNI GRCGYC 1 cut(s) 387
BssSI CACGAG 1 cut(s) 455
BssT1I CCWWGG 1 cut(s) 827
Bst2BI CACGAG 1 cut(s) 455
Bst2UI CCWGG 1 cut(s) 665
Bst4CI ACNGT 5 cut(s) 166, 417, 641, 755, 1094
Bst6I CTCTTC 2 cut(s) 274, 1186
BstACI GRCGYC 1 cut(s) 387
BstAFI CTTAAG 1 cut(s) 1183
BstAPI GCANNNNNTGC 2 cut(s) 81, 617
BstAUI TGTACA 1 cut(s) 1167
BstBAI YACGTR 1 cut(s) 173
BstC8I GCNNGC 4 cut(s) 843, 1046, 1152, 1245
BstDEI CTNAG 4 cut(s) 110, 603, 1022, 1352
BstF5I GGATG 2 cut(s) 918, 961
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 2 cut(s) 884, 1218
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 6 cut(s) 81, 212, 261, 617, 803, 1151
BstNI CCWGG 1 cut(s) 665
BstNSI RCATGY 2 cut(s) 636, 1048
BstSCI CCNGG 1 cut(s) 663
BstSFI CTRYAG 1 cut(s) 751
BstV1I GCAGC 7 cut(s) 340, 504, 604, 818, 959, 1051, 1154
BstV2I GAAGAC 1 cut(s) 1262
BsuI GTATCC 1 cut(s) 609
BsuRI GGCC 4 cut(s) 206, 264, 507, 960
BtrI CACGTC 1 cut(s) 1212
BtsCI GGATG 2 cut(s) 918, 961
BtsIMutI CAGTG 3 cut(s) 171, 646, 1099
Cac8I GCNNGC 4 cut(s) 843, 1046, 1152, 1245
Cfr10I RCCGGY 1 cut(s) 12
Csp6I GTAC 3 cut(s) 189, 1168, 1207
CviAII CATG 4 cut(s) 633, 1045, 1049, 1360
CviQI GTAC 3 cut(s) 189, 1168, 1207
DdeI CTNAG 4 cut(s) 110, 603, 1022, 1352
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
DraI TTTAAA 2 cut(s) 318, 1341
DraIII CACNNNGTG 1 cut(s) 815
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 2 cut(s) 274, 1186
EarI CTCTTC 2 cut(s) 274, 1186
Eco130I CCWWGG 1 cut(s) 827
Eco24I GRGCYC 1 cut(s) 1297
Eco72I CACGTG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 1290
EcoRII CCWGG 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 827
EcoT22I ATGCAT 1 cut(s) 1050
EcoT38I GRGCYC 1 cut(s) 1297
ErhI CCWWGG 1 cut(s) 827
Esp3I CGTCTC 1 cut(s) 1218
FaeI CATG 4 cut(s) 636, 1048, 1052, 1363
FalI AAGNNNNNCTT 6 cut(s) 64, 96, 606, 638, 1001, 1033
FaqI GGGAC 1 cut(s) 605
FatI CATG 4 cut(s) 632, 1044, 1048, 1359
Fnu4HI GCNGC 7 cut(s) 354, 493, 618, 807, 948, 1065, 1143
FokI GGATG 2 cut(s) 905, 968
FriOI GRGCYC 1 cut(s) 1297
Fsp4HI GCNGC 7 cut(s) 354, 493, 618, 807, 948, 1065, 1143
FspBI CTAG 1 cut(s) 1014
GluI GCNGC 7 cut(s) 354, 493, 618, 807, 948, 1065, 1143
GsaI CCCAGC 1 cut(s) 954
GsuI CTGGAG 1 cut(s) 686
HaeIII GGCC 4 cut(s) 206, 264, 507, 960
HapII CCGG 1 cut(s) 13
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 4 cut(s) 636, 1048, 1052, 1363
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 6 cut(s) 237, 368, 1097, 1174, 1258, 1356
HpaII CCGG 1 cut(s) 13
HphI GGTGA 4 cut(s) 125, 146, 713, 827
Hpy166II GTNNAC 3 cut(s) 400, 991, 1209
Hpy188I TCNGA 3 cut(s) 273, 434, 682
Hpy188III TCNNGA 2 cut(s) 655, 689
Hpy8I GTNNAC 3 cut(s) 400, 991, 1209
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 6 cut(s) 487, 582, 667, 734, 968, 1225
HpyCH4III ACNGT 5 cut(s) 166, 417, 641, 755, 1094
HpyCH4IV ACGT 4 cut(s) 172, 387, 499, 1211
HpyF10VI GCNNNNNNNGC 6 cut(s) 81, 212, 261, 617, 803, 1151
HpyF3I CTNAG 4 cut(s) 110, 603, 1022, 1352
HpySE526I ACGT 4 cut(s) 172, 387, 499, 1211
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 4 cut(s) 636, 1048, 1052, 1363
Kzo9I GATC 1 cut(s) 151
LguI GCTCTTC 1 cut(s) 1186
LmnI GCTCC 2 cut(s) 67, 838
Lsp1109I GCAGC 7 cut(s) 340, 504, 604, 818, 959, 1051, 1154
LweI GCATC 3 cut(s) 473, 1230, 1267
MaeI CTAG 1 cut(s) 1014
MaeII ACGT 4 cut(s) 172, 387, 499, 1211
MaeIII GTNAC 5 cut(s) 168, 447, 635, 714, 1003
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 5 cut(s) 291, 524, 682, 1203, 1267
MhlI GDGCHC 1 cut(s) 1297
MluCI AATT 4 cut(s) 509, 969, 1235, 1326
MlyI GAGTC 3 cut(s) 1168, 1252, 1350
MmeI TCCRAC 1 cut(s) 833
Mph1103I ATGCAT 1 cut(s) 1050
MroXI GAANNNNTTC 2 cut(s) 528, 696
MseI TTAA 8 cut(s) 60, 267, 317, 801, 980, 1089, 1184, 1340
MslI CAYNNNNRTG 2 cut(s) 245, 1266
MspA1I CMGCKG 1 cut(s) 950
MspCI CTTAAG 1 cut(s) 1183
MspI CCGG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 665
Mva1269I GAATGC 1 cut(s) 896
MvaI CCWGG 1 cut(s) 665
MwoI GCNNNNNNNGC 6 cut(s) 81, 212, 261, 617, 803, 1151
NdeII GATC 1 cut(s) 151
NlaIII CATG 4 cut(s) 636, 1048, 1052, 1363
NmuCI GTSAC 2 cut(s) 168, 714
NsiI ATGCAT 1 cut(s) 1050
NspI RCATGY 2 cut(s) 636, 1048
PaeI GCATGC 1 cut(s) 1048
PaeR7I CTCGAG 1 cut(s) 1290
PciSI GCTCTTC 1 cut(s) 1186
PcsI WCGNNNNNNNCGW 1 cut(s) 1297
PctI GAATGC 1 cut(s) 896
PdmI GAANNNNTTC 2 cut(s) 528, 696
PfeI GAWTC 3 cut(s) 237, 368, 1097
PflFI GACNNNGTC 1 cut(s) 166
PkrI GCNGC 7 cut(s) 355, 494, 619, 808, 949, 1066, 1144
PleI GAGTC 3 cut(s) 1168, 1252, 1350
PmaCI CACGTG 1 cut(s) 173
PmlI CACGTG 1 cut(s) 173
PpsI GAGTC 3 cut(s) 1168, 1252, 1350
Ppu21I YACGTR 1 cut(s) 173
PsiI TTATAA 1 cut(s) 1158
Psp6I CCWGG 1 cut(s) 663
PspCI CACGTG 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 950
PspGI CCWGG 1 cut(s) 663
PspXI VCTCGAGB 1 cut(s) 1290
PsyI GACNNNGTC 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 950
RsaI GTAC 3 cut(s) 190, 1169, 1208
RsaNI GTAC 3 cut(s) 189, 1168, 1207
RseI CAYNNNNRTG 2 cut(s) 245, 1266
SapI GCTCTTC 1 cut(s) 1186
SaqAI TTAA 8 cut(s) 60, 267, 317, 801, 980, 1089, 1184, 1340
SatI GCNGC 7 cut(s) 354, 493, 618, 807, 948, 1065, 1143
Sau3AI GATC 1 cut(s) 151
SchI GAGTC 3 cut(s) 1168, 1252, 1350
ScrFI CCNGG 1 cut(s) 665
SduI GDGCHC 1 cut(s) 1297
SfaNI GCATC 3 cut(s) 473, 1230, 1267
SfcI CTRYAG 1 cut(s) 751
Sfr274I CTCGAG 1 cut(s) 1290
SlaI CTCGAG 1 cut(s) 1290
SmiMI CAYNNNNRTG 2 cut(s) 245, 1266
SmlI CTYRAG 3 cut(s) 1183, 1215, 1290
SmoI CTYRAG 3 cut(s) 1183, 1215, 1290
SphI GCATGC 1 cut(s) 1048
Sse9I AATT 4 cut(s) 509, 969, 1235, 1326
SspMI CTAG 1 cut(s) 1014
StyD4I CCNGG 1 cut(s) 663
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 5 cut(s) 166, 417, 641, 755, 1094
TaiI ACGT 4 cut(s) 175, 390, 502, 1214
TaqI TCGA 3 cut(s) 210, 244, 1291
TasI AATT 4 cut(s) 509, 969, 1235, 1326
TatI WGTACW 3 cut(s) 188, 1167, 1206
TfiI GAWTC 3 cut(s) 237, 368, 1097
Tru1I TTAA 8 cut(s) 60, 267, 317, 801, 980, 1089, 1184, 1340
Tru9I TTAA 8 cut(s) 60, 267, 317, 801, 980, 1089, 1184, 1340
TscAI CASTG 3 cut(s) 171, 646, 1099
TseFI GTSAC 2 cut(s) 168, 714
TseI GCWGC 7 cut(s) 353, 492, 617, 806, 947, 1064, 1142
Tsp45I GTSAC 2 cut(s) 168, 714
TspDTI ATGAA 5 cut(s) 229, 381, 397, 889, 1089
TspGWI ACGGA 3 cut(s) 470, 525, 1289
TspRI CASTG 3 cut(s) 171, 646, 1099
Tth111I GACNNNGTC 1 cut(s) 166
Vha464I CTTAAG 1 cut(s) 1183
XceI RCATGY 2 cut(s) 636, 1048
XcmI CCANNNNNNNNNTGG 1 cut(s) 1066
XhoI CTCGAG 1 cut(s) 1290
XmnI GAANNNNTTC 2 cut(s) 528, 696
XspI CTAG 1 cut(s) 1014
ZraI GACGTC 1 cut(s) 388
Zsp2I ATGCAT 1 cut(s) 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.