Rw2G031120

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008507
Physical Location & Seq
Reverse (-)
51164970 .. 51167567
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031120.1

Sequence Viewer

Length: 1056 bp
ATGAGAAGAGATTTATGTGATGGTGTTGGTGTTGCCCGTCTTTTAAATGCAACTCTTGTTTTGCCAAAGTTTGAGGTGGCTGCTTATTGGAATGAATCAAGTGGTTTTGCAGATATCTTTGATGTCGACTACTTCATTCAACGGATGAATGGCTTCATCAGTGTTGTCAAAGAGTTACCATCTGAGATTGCATCAAAAGAACCCTTCCATGTGGATTGCAGCAAACGCAAAGGCCAATTTGATTACATAGAAAGTGTTCTTCCATCCCTATTAGAACATCATTATATTTCAATCACGCCAGCGATGAGCCAAAGAAGGGATAGGTATCCTCAGTATGCAAAAGCTGCCCTTTGCCAAGCATGTTACAGTGCTTTACGCCTCACAAAATCCCTGGAGAAGAAAGCCTCTGAGCTTCTTGATGCCATACCTAAACCTTTTCTCTCACTTCACCTTCGTTTTGAGCCTGATATGGTAGCTTACAGTCAATGTGAATATCCATACCTAGCTCCTGCTTCTATTGAAGCCATCAAGGCAGCACAGGGAGATAGAAAGCCATGGACCGGAGAGCTTGCCCAAATTTGGAGGAAACGAGGAAAATGTCCTCTTACGCCTAATGAGACAGCCTTCATACTTCAAGTGCTTTCCATCCCAACAAATACAAACATTTATCTGGCAGCTGGGGACGGTCTGATGGAAAGTGAAGGCTTAACATCTATTTACACCAATGTAGTTACCAAATCTAGCCTACTTAGTGGGGAGGATTTCAAAAGCATGCATGGAAACACGAAAGCGGCATTGGATTACCATGTGTCTATTAACAGTGATTCATATATAGCAACGTATTTTGGAAATATGGATAAGATGGTTGCAGCAATGCGAGCTTTTAATGGCTTGTACAAGACTCTCTTCTTAAGCAGAAGAGCTTTTGCTGAACTCACTTCTCAAGGTTTGAGAGGTAAGGAATTGGAGCATGCACTGTGGAAGGCTCACAAAGATGATTTTGTCATGGGAAGAGGATCTGCTTTGCCTGATTGCTTTTGTGAGTTCAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

351

Amino Acids

39.43

Weight (kDa)

7.57

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 1 - 295 3e-56 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 126
AciI CCGC 1 cut(s) 791
AclWI GGATC 1 cut(s) 1024
AcsI RAATTY 1 cut(s) 576
AfaI GTAC 1 cut(s) 896
AfiI CCNNNNNNNGG 3 cut(s) 316, 560, 579
AflII CTTAAG 1 cut(s) 910
AgsI TTSAA 6 cut(s) 140, 291, 521, 635, 766, 1048
AjnI CCWGG 1 cut(s) 390
AjuI GAANNNNNNNTTGG 2 cut(s) 779, 811
AluBI AGCT 8 cut(s) 344, 412, 476, 506, 568, 677, 881, 923
AluI AGCT 8 cut(s) 344, 412, 476, 506, 568, 677, 881, 923
Alw26I GTCTC 1 cut(s) 611
AlwI GGATC 1 cut(s) 1024
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 6 cut(s) 80, 219, 344, 533, 674, 869
ApoI RAATTY 1 cut(s) 576
Asp700I GAANNNNTTC 2 cut(s) 152, 255
AspS9I GGNCC 1 cut(s) 558
AsuHPI GGTGA 1 cut(s) 440
AvaII GGWCC 1 cut(s) 558
BbvI GCAGC 6 cut(s) 67, 231, 331, 545, 686, 881
BccI CCATC 7 cut(s) 14, 187, 271, 533, 653, 685, 856
BciT130I CCWGG 1 cut(s) 392
BciVI GTATCC 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 611
BfaI CTAG 2 cut(s) 503, 741
BfrI CTTAAG 1 cut(s) 910
BfuI GTATCC 1 cut(s) 336
BglI GCCNNNNNGGC 1 cut(s) 530
BisI GCNGC 7 cut(s) 81, 220, 345, 534, 675, 792, 870
BlsI GCNGC 7 cut(s) 82, 221, 346, 535, 676, 793, 871
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 558
BmrFI CCNGG 1 cut(s) 392
BmsI GCATC 2 cut(s) 200, 409
BpmI CTGGAG 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 927
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 390, 554
BsaWI WCCGGW 1 cut(s) 560
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 560, 579
Bse3DI GCAATG 1 cut(s) 879
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 2 cut(s) 390, 554
BseGI GGATG 3 cut(s) 150, 263, 645
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 3 cut(s) 316, 560, 579
BseMI GCAATG 1 cut(s) 879
BseMII CTCAG 3 cut(s) 174, 344, 399
BseXI GCAGC 6 cut(s) 67, 231, 331, 545, 686, 881
BseYI CCCAGC 1 cut(s) 677
BshFI GGCC 1 cut(s) 234
BsiSI CCGG 1 cut(s) 561
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 3 cut(s) 316, 560, 579
BsmAI GTCTC 1 cut(s) 611
BsmFI GGGAC 1 cut(s) 695
BsnI GGCC 1 cut(s) 234
Bsp1407I TGTACA 1 cut(s) 894
Bsp143I GATC 1 cut(s) 1016
Bsp19I CCATGG 1 cut(s) 554
BspACI CCGC 1 cut(s) 791
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 3 cut(s) 175, 343, 400
BspPI GGATC 1 cut(s) 1024
BspQI GCTCTTC 1 cut(s) 913
BspTI CTTAAG 1 cut(s) 910
BsrDI GCAATG 1 cut(s) 879
BsrGI TGTACA 1 cut(s) 894
BssECI CCNNGG 2 cut(s) 390, 554
BssMI GATC 1 cut(s) 1016
BssT1I CCWWGG 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 5 cut(s) 368, 482, 686, 821, 978
Bst6I CTCTTC 3 cut(s) 911, 913, 1006
BstAFI CTTAAG 1 cut(s) 910
BstAPI GCANNNNNTGC 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 894
BstC8I GCNNGC 5 cut(s) 300, 570, 773, 879, 972
BstDEI CTNAG 4 cut(s) 183, 330, 408, 749
BstDSI CCRYGG 1 cut(s) 554
BstF5I GGATG 3 cut(s) 150, 263, 645
BstKTI GATC 1 cut(s) 1019
BstMAI GTCTC 1 cut(s) 611
BstMBI GATC 1 cut(s) 1016
BstMWI GCNNNNNNNGC 4 cut(s) 225, 344, 530, 878
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 3 cut(s) 363, 775, 974
BstSCI CCNGG 1 cut(s) 390
BstV1I GCAGC 6 cut(s) 67, 231, 331, 545, 686, 881
BstX2I RGATCY 1 cut(s) 1016
BstYI RGATCY 1 cut(s) 1016
BsuI GTATCC 1 cut(s) 336
BsuRI GGCC 1 cut(s) 234
BtgI CCRYGG 1 cut(s) 554
BtgZI GCGATG 1 cut(s) 317
BtsCI GGATG 3 cut(s) 150, 263, 645
BtsIMutI CAGTG 4 cut(s) 166, 373, 826, 974
Cac8I GCNNGC 5 cut(s) 300, 570, 773, 879, 972
Cfr13I GGNCC 1 cut(s) 558
Csp6I GTAC 1 cut(s) 895
CviAII CATG 8 cut(s) 209, 360, 555, 772, 776, 806, 971, 1006
CviQI GTAC 1 cut(s) 895
DdeI CTNAG 4 cut(s) 183, 330, 408, 749
DpnI GATC 1 cut(s) 1018
DpnII GATC 1 cut(s) 1016
DraI TTTAAA 1 cut(s) 45
Eam1104I CTCTTC 3 cut(s) 911, 913, 1006
EarI CTCTTC 3 cut(s) 911, 913, 1006
Eco130I CCWWGG 1 cut(s) 554
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 390
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 554
EcoT22I ATGCAT 1 cut(s) 777
ErhI CCWWGG 1 cut(s) 554
FaeI CATG 8 cut(s) 212, 363, 558, 775, 779, 809, 974, 1009
FalI AAGNNNNNCTT 4 cut(s) 333, 365, 890, 922
FaqI GGGAC 1 cut(s) 695
FatI CATG 8 cut(s) 208, 359, 554, 771, 775, 805, 970, 1005
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 7 cut(s) 81, 220, 345, 534, 675, 792, 870
FokI GGATG 3 cut(s) 157, 250, 632
Fsp4HI GCNGC 7 cut(s) 81, 220, 345, 534, 675, 792, 870
FspBI CTAG 2 cut(s) 503, 741
GluI GCNGC 7 cut(s) 81, 220, 345, 534, 675, 792, 870
GsaI CCCAGC 1 cut(s) 681
GsuI CTGGAG 1 cut(s) 413
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 561
Hin1II CATG 8 cut(s) 212, 363, 558, 775, 779, 809, 974, 1009
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 3 cut(s) 95, 824, 901
HpaII CCGG 1 cut(s) 561
HphI GGTGA 1 cut(s) 440
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 3 cut(s) 184, 409, 690
Hpy188III TCNNGA 1 cut(s) 416
Hpy8I GTNNAC 1 cut(s) 127
HpyAV CCTTC 6 cut(s) 214, 309, 461, 634, 695, 976
HpyCH4III ACNGT 5 cut(s) 368, 482, 686, 821, 978
HpyCH4IV ACGT 1 cut(s) 839
HpyCH4V TGCA 8 cut(s) 50, 110, 191, 219, 338, 775, 869, 974
HpyF10VI GCNNNNNNNGC 4 cut(s) 225, 344, 530, 878
HpyF3I CTNAG 4 cut(s) 183, 330, 408, 749
HpySE526I ACGT 1 cut(s) 839
Hsp92II CATG 8 cut(s) 212, 363, 558, 775, 779, 809, 974, 1009
Kzo9I GATC 1 cut(s) 1016
LguI GCTCTTC 1 cut(s) 913
LmnI GCTCC 2 cut(s) 511, 967
Lsp1109I GCAGC 6 cut(s) 67, 231, 331, 545, 686, 881
LweI GCATC 2 cut(s) 200, 409
MaeI CTAG 2 cut(s) 503, 741
MaeII ACGT 1 cut(s) 839
MaeIII GTNAC 3 cut(s) 174, 362, 730
MalI GATC 1 cut(s) 1018
MboI GATC 1 cut(s) 1016
MboII GAAGA 6 cut(s) 18, 251, 409, 898, 930, 1023
MflI RGATCY 1 cut(s) 1016
MluCI AATT 4 cut(s) 236, 576, 962, 1049
MlyI GAGTC 1 cut(s) 895
Mph1103I ATGCAT 1 cut(s) 777
MroXI GAANNNNTTC 2 cut(s) 152, 255
MseI TTAA 5 cut(s) 44, 707, 816, 885, 911
MslI CAYNNNNRTG 1 cut(s) 993
MspA1I CMGCKG 1 cut(s) 677
MspCI CTTAAG 1 cut(s) 910
MspI CCGG 1 cut(s) 561
MspR9I CCNGG 1 cut(s) 392
MvaI CCWGG 1 cut(s) 392
MwoI GCNNNNNNNGC 4 cut(s) 225, 344, 530, 878
NcoI CCATGG 1 cut(s) 554
NdeII GATC 1 cut(s) 1016
NlaIII CATG 8 cut(s) 212, 363, 558, 775, 779, 809, 974, 1009
NsiI ATGCAT 1 cut(s) 777
NspI RCATGY 3 cut(s) 363, 775, 974
PaeI GCATGC 2 cut(s) 775, 974
PciSI GCTCTTC 1 cut(s) 913
PdmI GAANNNNTTC 2 cut(s) 152, 255
PfeI GAWTC 2 cut(s) 95, 824
PkrI GCNGC 7 cut(s) 82, 221, 346, 535, 676, 793, 871
PleI GAGTC 1 cut(s) 895
PpsI GAGTC 1 cut(s) 895
Psp6I CCWGG 1 cut(s) 390
PspFI CCCAGC 1 cut(s) 677
PspGI CCWGG 1 cut(s) 390
PspPI GGNCC 1 cut(s) 558
PsuI RGATCY 1 cut(s) 1016
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
RseI CAYNNNNRTG 1 cut(s) 993
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 913
SaqAI TTAA 5 cut(s) 44, 707, 816, 885, 911
SatI GCNGC 7 cut(s) 81, 220, 345, 534, 675, 792, 870
Sau3AI GATC 1 cut(s) 1016
Sau96I GGNCC 1 cut(s) 558
SchI GAGTC 1 cut(s) 895
ScrFI CCNGG 1 cut(s) 392
SfaNI GCATC 2 cut(s) 200, 409
SinI GGWCC 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 993
SmlI CTYRAG 2 cut(s) 910, 942
SmoI CTYRAG 2 cut(s) 910, 942
SphI GCATGC 2 cut(s) 775, 974
Sse9I AATT 4 cut(s) 236, 576, 962, 1049
SsiI CCGC 1 cut(s) 791
SspMI CTAG 2 cut(s) 503, 741
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 1 cut(s) 554
TaaI ACNGT 5 cut(s) 368, 482, 686, 821, 978
TaiI ACGT 1 cut(s) 842
TaqI TCGA 1 cut(s) 126
TasI AATT 4 cut(s) 236, 576, 962, 1049
TatI WGTACW 1 cut(s) 894
TauI GCSGC 1 cut(s) 794
TfiI GAWTC 2 cut(s) 95, 824
Tru1I TTAA 5 cut(s) 44, 707, 816, 885, 911
Tru9I TTAA 5 cut(s) 44, 707, 816, 885, 911
TscAI CASTG 4 cut(s) 166, 373, 826, 981
TseI GCWGC 6 cut(s) 80, 219, 344, 533, 674, 869
TspDTI ATGAA 6 cut(s) 108, 124, 145, 161, 616, 816
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 4 cut(s) 166, 373, 826, 981
Vha464I CTTAAG 1 cut(s) 910
VpaK11BI GGWCC 1 cut(s) 558
XapI RAATTY 1 cut(s) 576
XceI RCATGY 3 cut(s) 363, 775, 974
XmiI GTMKAC 1 cut(s) 126
XmnI GAANNNNTTC 2 cut(s) 152, 255
XspI CTAG 2 cut(s) 503, 741
Zsp2I ATGCAT 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.