AT1G53760

Mitochondrial K+-H+ exchange-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011394
Physical Location & Seq
Reverse (-)
20068965 .. 20071171
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53760.2

Sequence Viewer

Length: 819 bp
ATGAGAGCGAGATTGGTGGTTTTTCCGATCAAAGGGAAGAAATGGTGTTTCAGCAGATCTGTTGACCCTTTCGCTGCTCAATCACCGTCTGGTGTTACTCCGACCACTGTACGAGGTTTATGGAAGAAGATATCATCTGAATCGAAACCCATCAATGCCAACGCAGAGCTTCTCGTCGATTTCATCTCTGACAAGATGAATAAAGCTTGGGTGGGTCTTGAGAAAGCGCCTGATGGATCAATAAAGAACAAGATTCATGGGTTTGGATTGAAGCTTCTGGCTCGTGTTAAGCCTTCTGAGATTTTCTTGAAGTCTATATCTAAGGAGGTCACTTCTGTACAAGTCACTTATCCTCCGAGTTTAGATCCTAGACTCGTACGCAGGAGACTACGGCACATTGCCATGAGTGGAACAATCCTGCACAAGAAGTACCTAGTTGGTTCAGTTACTTTGCTTCCATTGACCAGTGCATTTATGGTATTGCCATTACCAAACATACCATTCTTCTGGGTCTTATTCCGCACATATTCTCACTGGCGGGCTCTTCAGGGAAGTGAGAAGCTGCTTAAGCTCATCTCAAACGAGGCAAATCCTGATAAACCAGACTCAACTGATGATGCAGATGAAAGCAAGAACAGCAACACCAAGCCGGAACAAAAATCTCAATCTCCCACTTGTATTTTGCTTCCATCGGAAGAGTTATATCAGCTAATTCGTGAAGCTAGCGAGGAAGGACTCGACGAGGCGACCATAATAGAGATTTGCAAATCCTTTGATCTCAATAAGAACGATGTTCTTAAATATCGCAATCTAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mit_KHE1

Protein Analysis

272

Amino Acids

30.61

Weight (kDa)

9.52

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mit_KHE1 PF10173 4 - 183 2e-40 Mitochondrial K+-H+ exchange-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53760 AT1G53760
fragaria_vesca FvH4_6g36070 FvH4_6g36070
malus_domestica MD09G1171800.v1.1 MD17G1155200.v1.1
prunus_persica Prupe.3G030200_v2.0.a1
pyrus_communis pycom09g08910 pycom17g14800
rosa_chinensis RchiOBHm_Chr2g0148141
rosa_laevigata RLG00000020320
rosa_multiflora Rmu_sc0001516.1_g000097 Rmu_sc0012028.1_g000004
rosa_roxburghii Rroxscaffold_2G00099050
rosa_rugosa Rorug02G0403900
rosa_samantha Rh2AG462500 Rh2BG475500 Rh2CG449300 Rh2DG484200
rosa_wichuraiana Rw2G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 520, 538
AclWI GGATC 2 cut(s) 244, 359
AcuI CTGAAG 1 cut(s) 530
AfaI GTAC 4 cut(s) 111, 339, 378, 431
AfiI CCNNNNNNNGG 1 cut(s) 32
AflII CTTAAG 1 cut(s) 566
AgsI TTSAA 2 cut(s) 271, 310
AluBI AGCT 7 cut(s) 169, 206, 274, 562, 571, 709, 722
AluI AGCT 7 cut(s) 169, 206, 274, 562, 571, 709, 722
Alw26I GTCTC 1 cut(s) 379
AlwI GGATC 2 cut(s) 244, 359
ApeKI GCWGC 2 cut(s) 74, 562
AspLEI GCGC 1 cut(s) 229
AsuHPI GGTGA 1 cut(s) 75
AsuNHI GCTAGC 1 cut(s) 722
BanII GRGCYC 1 cut(s) 544
BauI CACGAG 1 cut(s) 282
BbvI GCAGC 2 cut(s) 61, 549
BccI CCATC 3 cut(s) 158, 227, 697
BceAI ACGGC 1 cut(s) 407
BcoDI GTCTC 1 cut(s) 379
BfaI CTAG 4 cut(s) 369, 434, 723, 812
BfoI RGCGCY 1 cut(s) 230
BfrI CTTAAG 1 cut(s) 566
BglII AGATCT 1 cut(s) 56
BisI GCNGC 2 cut(s) 75, 563
BlsI GCNGC 2 cut(s) 76, 564
BmsI GCATC 1 cut(s) 607
BmtI GCTAGC 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 2 cut(s) 465, 539
Bse3DI GCAATG 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 2 cut(s) 465, 539
BseXI GCAGC 2 cut(s) 61, 549
BsgI GTGCAG 1 cut(s) 404
BsiSI CCGG 1 cut(s) 650
BsiWI CGTACG 1 cut(s) 376
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 544
Bsp1407I TGTACA 1 cut(s) 337
Bsp143I GATC 5 cut(s) 27, 56, 236, 364, 775
BspACI CCGC 2 cut(s) 520, 538
BspCNI CTCAG 1 cut(s) 289
BspOI GCTAGC 1 cut(s) 726
BspPI GGATC 2 cut(s) 244, 359
BspQI GCTCTTC 1 cut(s) 549
BspTI CTTAAG 1 cut(s) 566
BsrDI GCAATG 1 cut(s) 396
BsrGI TGTACA 1 cut(s) 337
BsrI ACTGG 2 cut(s) 465, 539
BssMI GATC 5 cut(s) 27, 56, 236, 364, 775
BssSI CACGAG 1 cut(s) 282
Bst2BI CACGAG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 87, 109
Bst6I CTCTTC 2 cut(s) 549, 690
BstAFI CTTAAG 1 cut(s) 566
BstAUI TGTACA 1 cut(s) 337
BstC8I GCNNGC 2 cut(s) 540, 724
BstDEI CTNAG 2 cut(s) 297, 321
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 5 cut(s) 30, 59, 239, 367, 778
BstMAI GTCTC 1 cut(s) 379
BstMBI GATC 5 cut(s) 27, 56, 236, 364, 775
BstMWI GCNNNNNNNGC 2 cut(s) 568, 636
BstV1I GCAGC 2 cut(s) 61, 549
BstX2I RGATCY 2 cut(s) 56, 364
BstXI CCANNNNNNTGG 1 cut(s) 507
BstYI RGATCY 2 cut(s) 56, 364
BtsIMutI CAGTG 3 cut(s) 105, 472, 532
Cac8I GCNNGC 2 cut(s) 540, 724
CfoI GCGC 1 cut(s) 229
Csp6I GTAC 4 cut(s) 110, 338, 377, 430
CviAII CATG 2 cut(s) 257, 403
CviQI GTAC 4 cut(s) 110, 338, 377, 430
DdeI CTNAG 2 cut(s) 297, 321
DpnI GATC 5 cut(s) 29, 58, 238, 366, 777
DpnII GATC 5 cut(s) 27, 56, 236, 364, 775
Eam1104I CTCTTC 2 cut(s) 549, 690
EarI CTCTTC 2 cut(s) 549, 690
Eco24I GRGCYC 1 cut(s) 544
Eco32I GATATC 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 530
EcoRV GATATC 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 544
FaeI CATG 2 cut(s) 260, 406
FaiI YATR 9 cut(s) 121, 258, 317, 404, 476, 497, 526, 703, 752
FatI CATG 2 cut(s) 256, 402
FauI CCCGC 1 cut(s) 531
Fnu4HI GCNGC 2 cut(s) 75, 563
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 2 cut(s) 75, 563
FspBI CTAG 4 cut(s) 369, 434, 723, 812
GlaI GCGC 1 cut(s) 228
GluI GCNGC 2 cut(s) 75, 563
HaeII RGCGCY 1 cut(s) 230
HapII CCGG 1 cut(s) 650
HhaI GCGC 1 cut(s) 229
Hin1II CATG 2 cut(s) 260, 406
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HindIII AAGCTT 2 cut(s) 204, 272
HinfI GANTC 5 cut(s) 140, 253, 372, 605, 735
HpaII CCGG 1 cut(s) 650
HphI GGTGA 1 cut(s) 75
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 7 cut(s) 27, 102, 139, 190, 298, 357, 694
Hpy188III TCNNGA 4 cut(s) 218, 307, 593, 716
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 2 cut(s) 179, 743
HpyAV CCTTC 2 cut(s) 303, 725
HpyCH4III ACNGT 2 cut(s) 87, 109
HpyCH4V TGCA 4 cut(s) 421, 470, 620, 765
HpyF10VI GCNNNNNNNGC 2 cut(s) 568, 636
HpyF3I CTNAG 2 cut(s) 297, 321
Hsp92II CATG 2 cut(s) 260, 406
HspAI GCGC 1 cut(s) 227
Kzo9I GATC 5 cut(s) 27, 56, 236, 364, 775
LguI GCTCTTC 1 cut(s) 549
Lsp1109I GCAGC 2 cut(s) 61, 549
LweI GCATC 1 cut(s) 607
MaeI CTAG 4 cut(s) 369, 434, 723, 812
MaeIII GTNAC 4 cut(s) 94, 328, 343, 445
MalI GATC 5 cut(s) 29, 58, 238, 366, 777
MboI GATC 5 cut(s) 27, 56, 236, 364, 775
MboII GAAGA 6 cut(s) 49, 136, 139, 496, 536, 707
MflI RGATCY 2 cut(s) 56, 364
MhlI GDGCHC 1 cut(s) 544
MluCI AATT 1 cut(s) 711
MlyI GAGTC 3 cut(s) 366, 599, 729
MmeI TCCRAC 1 cut(s) 125
MnlI CCTC 6 cut(s) 107, 319, 363, 577, 721, 736
MseI TTAA 3 cut(s) 288, 567, 798
MslI CAYNNNNRTG 1 cut(s) 401
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 1 cut(s) 650
MwoI GCNNNNNNNGC 2 cut(s) 568, 636
NdeII GATC 5 cut(s) 27, 56, 236, 364, 775
NheI GCTAGC 1 cut(s) 722
NlaIII CATG 2 cut(s) 260, 406
NmuCI GTSAC 2 cut(s) 328, 343
PciSI GCTCTTC 1 cut(s) 549
PfeI GAWTC 2 cut(s) 140, 253
Pfl23II CGTACG 1 cut(s) 376
PkrI GCNGC 2 cut(s) 76, 564
PleI GAGTC 3 cut(s) 366, 599, 729
PpsI GAGTC 3 cut(s) 366, 599, 729
PspLI CGTACG 1 cut(s) 376
PsuI RGATCY 2 cut(s) 56, 364
RsaI GTAC 4 cut(s) 111, 339, 378, 431
RsaNI GTAC 4 cut(s) 110, 338, 377, 430
RseI CAYNNNNRTG 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 3 cut(s) 288, 567, 798
SatI GCNGC 2 cut(s) 75, 563
Sau3AI GATC 5 cut(s) 27, 56, 236, 364, 775
SchI GAGTC 3 cut(s) 366, 599, 729
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 1 cut(s) 607
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 2 cut(s) 218, 566
SmoI CTYRAG 2 cut(s) 218, 566
Sse9I AATT 1 cut(s) 711
SsiI CCGC 2 cut(s) 520, 538
SspMI CTAG 4 cut(s) 369, 434, 723, 812
TaaI ACNGT 2 cut(s) 87, 109
TaqI TCGA 3 cut(s) 143, 177, 738
TasI AATT 1 cut(s) 711
TatI WGTACW 1 cut(s) 337
TfiI GAWTC 2 cut(s) 140, 253
Tru1I TTAA 3 cut(s) 288, 567, 798
Tru9I TTAA 3 cut(s) 288, 567, 798
TscAI CASTG 3 cut(s) 112, 472, 539
TseFI GTSAC 2 cut(s) 328, 343
TseI GCWGC 2 cut(s) 74, 562
Tsp45I GTSAC 2 cut(s) 328, 343
TspDTI ATGAA 4 cut(s) 172, 212, 245, 639
TspRI CASTG 3 cut(s) 112, 472, 539
Vha464I CTTAAG 1 cut(s) 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XspI CTAG 4 cut(s) 369, 434, 723, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.