Rh2CG449300

Mitochondrial K+-H+ exchange-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011394
Physical Location & Seq
Forward (+)
61014592 .. 61017695
3104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG449300.1

Sequence Viewer

Length: 828 bp
ATGAGAGCGAGATTGATAGTGTTTCCTGTTAAAGGGAGGAACTGGTGTTTTAGCAGATCAATTGATCCTCTGGTTTCGGATTCTGGTTCTGGAAATACTCCTTCCACTCTCAAAGATCTCTGGAAAAAGATCTCTTTCAATTCCAATCCCAATGTTAATTCTAGCTCCAATGCCAATGTCAATCCCTTTGCTGCTAATGCTGAACTGGTAGTAGATTTTATCTCGAACAAGATGAACAAAGCTTGGATTAGTCTGGAGAAGGCGCCTGAGGGAACCTTTAAAAGAAAGATTCATGGGGTGGGATTGAAGCTATTGGCTCGGGTGAAGCCGTCTGAGATTTTCTTGAAATCTATCACTAACGAGGTCTCACATGTTGAAGTTACATACCCTTCAAGCTTAAATGCACGACTTGTGCGTAGGAGGTTACGACATATTGCCTCGAGGGGATCTATCATACACAAGAAGTACTTATACGGCTCCGTTACGTTGCTCCCTCTAACATCTGCATTTATGGTTTTGCCTTTGCCTAATATTCCCTTCTTCTGGTGTTTATTCCGTACTTACTCTCATTGGCGAGCTCTCCAGGGAAGTGAGAAGCTTCTTCAGCTAGTCTCAGATAGCCTGAAGACTCCAGATGGAAATGGAGTTGAGCATGGAACGAAAAAGTTACTTAATTCTCTGTGGGTTTTGCAGCCATCCAAAGAACTTGAGGAACTTCTTCATCAGGGAGATGAACACGATGATCTGAATAAGCAGGCTATATTAAAAGTCTGCAAAGCCTTTGATTTGAACTCGAATGATGTTCTAAAATACAGAGATACCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mit_KHE1

Protein Analysis

275

Amino Acids

31.02

Weight (kDa)

9.77

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mit_KHE1 PF10173 4 - 204 1.5e-43 Mitochondrial K+-H+ exchange-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53760 AT1G53760
fragaria_vesca FvH4_6g36070 FvH4_6g36070
malus_domestica MD09G1171800.v1.1 MD17G1155200.v1.1
prunus_persica Prupe.3G030200_v2.0.a1
pyrus_communis pycom09g08910 pycom17g14800
rosa_chinensis RchiOBHm_Chr2g0148141
rosa_laevigata RLG00000020320
rosa_multiflora Rmu_sc0001516.1_g000097 Rmu_sc0012028.1_g000004
rosa_roxburghii Rroxscaffold_2G00099050
rosa_rugosa Rorug02G0403900
rosa_samantha Rh2AG462500 Rh2BG475500 Rh2CG449300 Rh2DG484200
rosa_wichuraiana Rw2G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 439
AccB1I GGYRCC 1 cut(s) 262
AclWI GGATC 2 cut(s) 59, 454
AcuI CTGAAG 2 cut(s) 587, 644
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 2 cut(s) 467, 559
AfiI CCNNNNNNNGG 2 cut(s) 32, 543
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 6 cut(s) 139, 307, 346, 377, 393, 790
AjnI CCWGG 1 cut(s) 582
AluBI AGCT 7 cut(s) 165, 242, 310, 396, 578, 598, 607
AluI AGCT 7 cut(s) 165, 242, 310, 396, 578, 598, 607
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 2 cut(s) 370, 616
AlwI GGATC 2 cut(s) 59, 454
Ama87I CYCGRG 2 cut(s) 318, 439
ApeKI GCWGC 2 cut(s) 191, 691
Asp700I GAANNNNTTC 1 cut(s) 717
AspLEI GCGC 1 cut(s) 265
AsuHPI GGTGA 1 cut(s) 334
AvaI CYCGRG 2 cut(s) 318, 439
AxyI CCTNAGG 1 cut(s) 267
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 580
BarI GAAGNNNNNNTAC 2 cut(s) 455, 487
BbsI GAAGAC 1 cut(s) 632
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 2 cut(s) 178, 703
BccI CCATC 2 cut(s) 629, 703
BceAI ACGGC 2 cut(s) 313, 490
BciT130I CCWGG 1 cut(s) 584
BcoDI GTCTC 2 cut(s) 370, 616
BfaI CTAG 2 cut(s) 162, 608
BfoI RGCGCY 1 cut(s) 266
BglII AGATCT 2 cut(s) 115, 129
BisI GCNGC 2 cut(s) 192, 692
BlsI GCNGC 2 cut(s) 193, 693
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 1 cut(s) 584
BmeT110I CYCGRG 2 cut(s) 318, 439
BmiI GGNNCC 3 cut(s) 264, 274, 478
BmrFI CCNGG 1 cut(s) 584
BpiI GAAGAC 1 cut(s) 632
BpmI CTGGAG 3 cut(s) 275, 566, 615
BpuEI CTTGAG 1 cut(s) 728
BsaHI GRCGYC 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 370
BsaJI CCNNGG 1 cut(s) 583
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 543
Bse1I ACTGG 2 cut(s) 47, 210
Bse21I CCTNAGG 1 cut(s) 267
BseBI CCWGG 1 cut(s) 584
BseDI CCNNGG 1 cut(s) 583
BseGI GGATG 1 cut(s) 695
BseLI CCNNNNNNNGG 2 cut(s) 32, 543
BseMII CTCAG 3 cut(s) 258, 324, 627
BseNI ACTGG 2 cut(s) 47, 210
BseXI GCAGC 2 cut(s) 178, 703
BshNI GGYRCC 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 580
BsiHKCI CYCGRG 2 cut(s) 318, 439
BslI CCNNNNNNNGG 2 cut(s) 32, 543
BsmAI GTCTC 2 cut(s) 370, 616
Bso31I GGTCTC 1 cut(s) 370
BsoBI CYCGRG 2 cut(s) 318, 439
Bsp1286I GDGCHC 1 cut(s) 580
Bsp143I GATC 6 cut(s) 56, 64, 115, 129, 446, 742
BspCNI CTCAG 3 cut(s) 259, 325, 626
BspLI GGNNCC 3 cut(s) 264, 274, 478
BspPI GGATC 2 cut(s) 59, 454
BspT107I GGYRCC 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 370
BsrI ACTGG 2 cut(s) 47, 210
BssECI CCNNGG 1 cut(s) 583
BssMI GATC 6 cut(s) 56, 64, 115, 129, 446, 742
BssNI GRCGYC 1 cut(s) 263
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 1 cut(s) 823
BstACI GRCGYC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 576, 756
BstDEI CTNAG 3 cut(s) 267, 333, 613
BstENI CCTNNNNNAGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 695
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 6 cut(s) 59, 67, 118, 132, 449, 745
BstMAI GTCTC 2 cut(s) 370, 616
BstMBI GATC 6 cut(s) 56, 64, 115, 129, 446, 742
BstMWI GCNNNNNNNGC 2 cut(s) 197, 604
BstNI CCWGG 1 cut(s) 584
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 582
BstV1I GCAGC 2 cut(s) 178, 703
BstV2I GAAGAC 1 cut(s) 632
BstX2I RGATCY 3 cut(s) 115, 129, 446
BstYI RGATCY 3 cut(s) 115, 129, 446
Bsu36I CCTNAGG 1 cut(s) 267
BtsCI GGATG 1 cut(s) 695
Cac8I GCNNGC 2 cut(s) 576, 756
CfoI GCGC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 466, 558
CviAII CATG 3 cut(s) 293, 371, 653
CviQI GTAC 2 cut(s) 466, 558
DdeI CTNAG 3 cut(s) 267, 333, 613
DinI GGCGCC 1 cut(s) 264
DpnI GATC 6 cut(s) 58, 66, 117, 131, 448, 744
DpnII GATC 6 cut(s) 56, 64, 115, 129, 446, 742
DraI TTTAAA 1 cut(s) 280
Ecl136II GAGCTC 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 580
Eco31I GGTCTC 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 578
Eco57I CTGAAG 2 cut(s) 587, 644
Eco81I CCTNAGG 1 cut(s) 267
Eco88I CYCGRG 2 cut(s) 318, 439
EcoICRI GAGCTC 1 cut(s) 578
EcoNI CCTNNNNNAGG 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 582
EcoT38I GRGCYC 1 cut(s) 580
EgeI GGCGCC 1 cut(s) 264
EheI GGCGCC 1 cut(s) 264
FaeI CATG 3 cut(s) 296, 374, 656
FaiI YATR 9 cut(s) 294, 372, 385, 432, 455, 472, 512, 654, 761
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FatI CATG 3 cut(s) 292, 370, 652
Fnu4HI GCNGC 2 cut(s) 192, 692
FokI GGATG 1 cut(s) 682
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 2 cut(s) 192, 692
FspBI CTAG 2 cut(s) 162, 608
GlaI GCGC 1 cut(s) 264
GluI GCNGC 2 cut(s) 192, 692
GsuI CTGGAG 3 cut(s) 275, 566, 615
HaeII RGCGCY 1 cut(s) 266
HhaI GCGC 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 3 cut(s) 296, 374, 656
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HindIII AAGCTT 3 cut(s) 240, 394, 596
HinfI GANTC 3 cut(s) 80, 289, 628
HphI GGTGA 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 79, 334, 616, 747
Hpy188III TCNNGA 6 cut(s) 90, 121, 223, 254, 343, 632
HpyAV CCTTC 4 cut(s) 111, 253, 399, 547
HpyCH4III ACNGT 1 cut(s) 823
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 4 cut(s) 404, 506, 691, 774
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 604
HpyF3I CTNAG 3 cut(s) 267, 333, 613
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 3 cut(s) 296, 374, 656
HspAI GCGC 1 cut(s) 263
KasI GGCGCC 1 cut(s) 262
Kzo9I GATC 6 cut(s) 56, 64, 115, 129, 446, 742
LmnI GCTCC 3 cut(s) 170, 482, 495
Lsp1109I GCAGC 2 cut(s) 178, 703
MaeI CTAG 2 cut(s) 162, 608
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 4 cut(s) 379, 423, 481, 666
MalI GATC 6 cut(s) 58, 66, 117, 131, 448, 744
MboI GATC 6 cut(s) 56, 64, 115, 129, 446, 742
MboII GAAGA 4 cut(s) 532, 593, 637, 710
MfeI CAATTG 1 cut(s) 60
MflI RGATCY 3 cut(s) 115, 129, 446
MhlI GDGCHC 1 cut(s) 580
MluCI AATT 4 cut(s) 60, 139, 157, 673
Mly113I GGCGCC 1 cut(s) 263
MlyI GAGTC 1 cut(s) 622
MnlI CCTC 9 cut(s) 30, 78, 262, 355, 414, 435, 448, 504, 703
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 6 cut(s) 30, 156, 279, 398, 672, 764
MspR9I CCNGG 1 cut(s) 584
MunI CAATTG 1 cut(s) 60
MvaI CCWGG 1 cut(s) 584
MwoI GCNNNNNNNGC 2 cut(s) 197, 604
NarI GGCGCC 1 cut(s) 263
NdeII GATC 6 cut(s) 56, 64, 115, 129, 446, 742
NlaIII CATG 3 cut(s) 296, 374, 656
NlaIV GGNNCC 3 cut(s) 264, 274, 478
NspI RCATGY 1 cut(s) 374
PaeR7I CTCGAG 1 cut(s) 439
PciI ACATGT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 2 cut(s) 80, 289
PkrI GCNGC 2 cut(s) 193, 693
PleI GAGTC 1 cut(s) 622
PluTI GGCGCC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 622
PscI ACATGT 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 582
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 3 cut(s) 264, 274, 478
PspXI VCTCGAGB 1 cut(s) 439
PsuI RGATCY 3 cut(s) 115, 129, 446
RsaI GTAC 2 cut(s) 467, 559
RsaNI GTAC 2 cut(s) 466, 558
SacI GAGCTC 1 cut(s) 580
SaqAI TTAA 6 cut(s) 30, 156, 279, 398, 672, 764
SatI GCNGC 2 cut(s) 192, 692
Sau3AI GATC 6 cut(s) 56, 64, 115, 129, 446, 742
ScaI AGTACT 1 cut(s) 467
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 1 cut(s) 584
SduI GDGCHC 1 cut(s) 580
SfoI GGCGCC 1 cut(s) 264
Sfr274I CTCGAG 1 cut(s) 439
SlaI CTCGAG 1 cut(s) 439
SmlI CTYRAG 2 cut(s) 439, 707
SmoI CTYRAG 2 cut(s) 439, 707
Sse9I AATT 4 cut(s) 60, 139, 157, 673
SspDI GGCGCC 1 cut(s) 262
SspI AATATT 1 cut(s) 532
SspMI CTAG 2 cut(s) 162, 608
SstI GAGCTC 1 cut(s) 580
StyD4I CCNGG 1 cut(s) 582
TaaI ACNGT 1 cut(s) 823
TaiI ACGT 1 cut(s) 488
TaqI TCGA 3 cut(s) 224, 440, 794
TasI AATT 4 cut(s) 60, 139, 157, 673
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 2 cut(s) 80, 289
Tru1I TTAA 6 cut(s) 30, 156, 279, 398, 672, 764
Tru9I TTAA 6 cut(s) 30, 156, 279, 398, 672, 764
TseI GCWGC 2 cut(s) 191, 691
TspDTI ATGAA 4 cut(s) 248, 281, 710, 747
TspGWI ACGGA 2 cut(s) 469, 545
XagI CCTNNNNNAGG 1 cut(s) 30
XceI RCATGY 1 cut(s) 374
XhoI CTCGAG 1 cut(s) 439
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 2 cut(s) 162, 608
ZrmI AGTACT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.