RLG00000020320

Mitochondrial K+-H+ exchange-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011394
Physical Location & Seq
Forward (+)
63691375 .. 63694502
3128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020320

Sequence Viewer

Length: 834 bp
ATGAGAGCGAGATTGATAGTGTTTCCTGTTAAAGGGAGGAACTGGTGTTTTAGCAGATCAATTGATCCTCTGGTTTCGGATTCTGGTGCTGGTAATACTCCTTCCACTCTCAAAGATCTCTGGAAAAGGATCTCTTTCAATTCAAATCCCAACGTTAATTCTAGTTCCAATGCCAATGCCACTGTCAATCCCTTTGCTGCTAATGCTGAACTGGTAGTCGATTTCGTATCTAACAAGATGAACAAAGCTTGGATTAGTCTGGAGAAGGCGCCTGAGGGAACCTTTAAAAGAAAAATTCATGGGTTGGGATTGAAGCTATTGGCTCGGGTGAAGCCGTCTGAGATTTTCTTGAAATCTATCACTAACGAGGTCTCACATGTTGAAGTTACATACCCTTCAAGCTTAAATGCACGACTTGTGCGTAGGAGGTTACGACATATTGCCTCGAGGGGATCTATCATACACAAGAAGTACTTATACGGTTCGGTTACGTTGCTCCCTCTGACATCTGCATTTATGGTTTTGCCTTTGCCTAATATTCCCTTCTTCTGGTGTTTATTCCGTACTTACTCTCATTGGCGAGCTCTCCAGGGAAGCGAGAAGCTTCTTCAGCTAGTCTCAGATAGGCCGAAGACTCCACATGGAAATGGAGTTGAGCATGGAACGAAAAAGTTACTTAATTCTCCGTGGGTTTTGCAGCCATCCAAAGAACTTGAGGAACTTCTTCATCAGGGAGATGAACACGATGATCTGAATAAGCAGGCTATATTAAAAATCTGCAAAGCCTTTGATTTGAACTCGAATGATGTTCTAAAATACAGAGATACCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mit_KHE1

Protein Analysis

278

Amino Acids

31.31

Weight (kDa)

9.92

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mit_KHE1 PF10173 4 - 206 1.2e-43 Mitochondrial K+-H+ exchange-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53760 AT1G53760
fragaria_vesca FvH4_6g36070 FvH4_6g36070
malus_domestica MD09G1171800.v1.1 MD17G1155200.v1.1
prunus_persica Prupe.3G030200_v2.0.a1
pyrus_communis pycom09g08910 pycom17g14800
rosa_chinensis RchiOBHm_Chr2g0148141
rosa_laevigata RLG00000020320
rosa_multiflora Rmu_sc0001516.1_g000097 Rmu_sc0012028.1_g000004
rosa_roxburghii Rroxscaffold_2G00099050
rosa_rugosa Rorug02G0403900
rosa_samantha Rh2AG462500 Rh2BG475500 Rh2CG449300 Rh2DG484200
rosa_wichuraiana Rw2G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 445
AccB1I GGYRCC 1 cut(s) 268
AclI AACGTT 1 cut(s) 153
AclWI GGATC 3 cut(s) 59, 137, 460
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 593
AcyI GRCGYC 1 cut(s) 269
AfaI GTAC 2 cut(s) 473, 565
AfiI CCNNNNNNNGG 2 cut(s) 32, 549
AflIII ACRYGT 1 cut(s) 376
AgsI TTSAA 7 cut(s) 139, 144, 313, 352, 383, 399, 796
AjnI CCWGG 1 cut(s) 588
AluBI AGCT 6 cut(s) 248, 316, 402, 584, 604, 613
AluI AGCT 6 cut(s) 248, 316, 402, 584, 604, 613
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 2 cut(s) 376, 622
AlwI GGATC 3 cut(s) 59, 137, 460
Ama87I CYCGRG 2 cut(s) 324, 445
AoxI GGCC 1 cut(s) 626
ApeKI GCWGC 2 cut(s) 197, 697
ApoI RAATTY 1 cut(s) 294
Asp700I GAANNNNTTC 1 cut(s) 723
AspLEI GCGC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 340
AvaI CYCGRG 2 cut(s) 324, 445
AxyI CCTNAGG 1 cut(s) 273
BanI GGYRCC 1 cut(s) 268
BanII GRGCYC 1 cut(s) 586
BarI GAAGNNNNNNTAC 4 cut(s) 85, 117, 461, 493
BbsI GAAGAC 1 cut(s) 638
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 2 cut(s) 184, 709
BccI CCATC 1 cut(s) 709
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 1 cut(s) 590
BcoDI GTCTC 2 cut(s) 376, 622
BfaI CTAG 2 cut(s) 162, 614
BfoI RGCGCY 1 cut(s) 272
BglII AGATCT 1 cut(s) 115
BisI GCNGC 2 cut(s) 198, 698
BlsI GCNGC 2 cut(s) 199, 699
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 1 cut(s) 590
BmeT110I CYCGRG 2 cut(s) 324, 445
BmiI GGNNCC 2 cut(s) 270, 280
BmrFI CCNGG 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 638
BpmI CTGGAG 2 cut(s) 281, 572
BpuEI CTTGAG 1 cut(s) 734
BsaHI GRCGYC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 376
BsaJI CCNNGG 2 cut(s) 589, 686
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 549
Bse1I ACTGG 2 cut(s) 47, 216
Bse21I CCTNAGG 1 cut(s) 273
BseBI CCWGG 1 cut(s) 590
BseDI CCNNGG 2 cut(s) 589, 686
BseGI GGATG 1 cut(s) 701
BseLI CCNNNNNNNGG 2 cut(s) 32, 549
BseMII CTCAG 3 cut(s) 264, 330, 633
BseNI ACTGG 2 cut(s) 47, 216
BseXI GCAGC 2 cut(s) 184, 709
BshFI GGCC 1 cut(s) 628
BshNI GGYRCC 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 586
BsiHKCI CYCGRG 2 cut(s) 324, 445
BslI CCNNNNNNNGG 2 cut(s) 32, 549
BsmAI GTCTC 2 cut(s) 376, 622
BsnI GGCC 1 cut(s) 628
Bso31I GGTCTC 1 cut(s) 376
BsoBI CYCGRG 2 cut(s) 324, 445
Bsp1286I GDGCHC 1 cut(s) 586
Bsp143I GATC 6 cut(s) 56, 64, 115, 129, 452, 748
BspANI GGCC 1 cut(s) 628
BspCNI CTCAG 3 cut(s) 265, 331, 632
BspLI GGNNCC 2 cut(s) 270, 280
BspPI GGATC 3 cut(s) 59, 137, 460
BspT107I GGYRCC 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 376
BsrI ACTGG 2 cut(s) 47, 216
BssECI CCNNGG 2 cut(s) 589, 686
BssMI GATC 6 cut(s) 56, 64, 115, 129, 452, 748
BssNI GRCGYC 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 2 cut(s) 184, 482
BstACI GRCGYC 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 582, 762
BstDEI CTNAG 3 cut(s) 273, 339, 619
BstDSI CCRYGG 1 cut(s) 686
BstENI CCTNNNNNAGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 701
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 6 cut(s) 59, 67, 118, 132, 455, 751
BstMAI GTCTC 2 cut(s) 376, 622
BstMBI GATC 6 cut(s) 56, 64, 115, 129, 452, 748
BstMWI GCNNNNNNNGC 2 cut(s) 203, 610
BstNI CCWGG 1 cut(s) 590
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 588
BstV1I GCAGC 2 cut(s) 184, 709
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 3 cut(s) 115, 129, 452
BstYI RGATCY 3 cut(s) 115, 129, 452
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 1 cut(s) 628
BtgI CCRYGG 1 cut(s) 686
BtsCI GGATG 1 cut(s) 701
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 2 cut(s) 582, 762
CfoI GCGC 1 cut(s) 271
Csp6I GTAC 2 cut(s) 472, 564
CviAII CATG 5 cut(s) 299, 377, 641, 659, 829
CviQI GTAC 2 cut(s) 472, 564
DdeI CTNAG 3 cut(s) 273, 339, 619
DinI GGCGCC 1 cut(s) 270
DpnI GATC 6 cut(s) 58, 66, 117, 131, 454, 750
DpnII GATC 6 cut(s) 56, 64, 115, 129, 452, 748
DraI TTTAAA 1 cut(s) 286
Ecl136II GAGCTC 1 cut(s) 584
Eco24I GRGCYC 1 cut(s) 586
Eco31I GGTCTC 1 cut(s) 376
Eco53kI GAGCTC 1 cut(s) 584
Eco57I CTGAAG 1 cut(s) 593
Eco81I CCTNAGG 1 cut(s) 273
Eco88I CYCGRG 2 cut(s) 324, 445
EcoICRI GAGCTC 1 cut(s) 584
EcoNI CCTNNNNNAGG 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 588
EcoT38I GRGCYC 1 cut(s) 586
EgeI GGCGCC 1 cut(s) 270
EheI GGCGCC 1 cut(s) 270
FaeI CATG 5 cut(s) 302, 380, 644, 662, 832
FalI AAGNNNNNCTT 4 cut(s) 118, 150, 458, 490
FatI CATG 5 cut(s) 298, 376, 640, 658, 828
Fnu4HI GCNGC 2 cut(s) 198, 698
FokI GGATG 1 cut(s) 688
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 2 cut(s) 198, 698
FspBI CTAG 2 cut(s) 162, 614
GlaI GCGC 1 cut(s) 270
GluI GCNGC 2 cut(s) 198, 698
GsuI CTGGAG 2 cut(s) 281, 572
HaeII RGCGCY 1 cut(s) 272
HaeIII GGCC 1 cut(s) 628
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 5 cut(s) 302, 380, 644, 662, 832
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HindIII AAGCTT 3 cut(s) 246, 400, 602
HinfI GANTC 2 cut(s) 80, 634
HphI GGTGA 1 cut(s) 340
Hpy188I TCNGA 5 cut(s) 79, 340, 504, 622, 753
Hpy188III TCNNGA 3 cut(s) 121, 260, 349
HpyAV CCTTC 4 cut(s) 111, 259, 405, 553
HpyCH4III ACNGT 2 cut(s) 184, 482
HpyCH4IV ACGT 2 cut(s) 153, 491
HpyCH4V TGCA 4 cut(s) 410, 512, 697, 780
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 610
HpyF3I CTNAG 3 cut(s) 273, 339, 619
HpySE526I ACGT 2 cut(s) 153, 491
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 5 cut(s) 302, 380, 644, 662, 832
HspAI GCGC 1 cut(s) 269
KasI GGCGCC 1 cut(s) 268
Kzo9I GATC 6 cut(s) 56, 64, 115, 129, 452, 748
LmnI GCTCC 1 cut(s) 501
Lsp1109I GCAGC 2 cut(s) 184, 709
MaeI CTAG 2 cut(s) 162, 614
MaeII ACGT 2 cut(s) 153, 491
MaeIII GTNAC 4 cut(s) 385, 429, 487, 672
MalI GATC 6 cut(s) 58, 66, 117, 131, 454, 750
MboI GATC 6 cut(s) 56, 64, 115, 129, 452, 748
MboII GAAGA 4 cut(s) 538, 599, 643, 716
MfeI CAATTG 1 cut(s) 60
MflI RGATCY 3 cut(s) 115, 129, 452
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 5 cut(s) 60, 139, 157, 294, 679
Mly113I GGCGCC 1 cut(s) 269
MlyI GAGTC 1 cut(s) 628
MnlI CCTC 9 cut(s) 30, 78, 268, 361, 420, 441, 454, 510, 709
MroXI GAANNNNTTC 1 cut(s) 723
MseI TTAA 6 cut(s) 30, 156, 285, 404, 678, 770
MslI CAYNNNNRTG 1 cut(s) 645
MspR9I CCNGG 1 cut(s) 590
MunI CAATTG 1 cut(s) 60
MvaI CCWGG 1 cut(s) 590
MwoI GCNNNNNNNGC 2 cut(s) 203, 610
NarI GGCGCC 1 cut(s) 269
NdeII GATC 6 cut(s) 56, 64, 115, 129, 452, 748
NlaIII CATG 5 cut(s) 302, 380, 644, 662, 832
NlaIV GGNNCC 2 cut(s) 270, 280
NspI RCATGY 1 cut(s) 380
PaeR7I CTCGAG 1 cut(s) 445
PciI ACATGT 1 cut(s) 376
PcsI WCGNNNNNNNCGW 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 723
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 2 cut(s) 199, 699
PleI GAGTC 1 cut(s) 628
PluTI GGCGCC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 628
PscI ACATGT 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 586
Psp1406I AACGTT 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 588
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 2 cut(s) 270, 280
PspXI VCTCGAGB 1 cut(s) 445
PsuI RGATCY 3 cut(s) 115, 129, 452
RsaI GTAC 2 cut(s) 473, 565
RsaNI GTAC 2 cut(s) 472, 564
RseI CAYNNNNRTG 1 cut(s) 645
SacI GAGCTC 1 cut(s) 586
SaqAI TTAA 6 cut(s) 30, 156, 285, 404, 678, 770
SatI GCNGC 2 cut(s) 198, 698
Sau3AI GATC 6 cut(s) 56, 64, 115, 129, 452, 748
ScaI AGTACT 1 cut(s) 473
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 1 cut(s) 590
SduI GDGCHC 1 cut(s) 586
SfoI GGCGCC 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 445
SlaI CTCGAG 1 cut(s) 445
SmiMI CAYNNNNRTG 1 cut(s) 645
SmlI CTYRAG 2 cut(s) 445, 713
SmoI CTYRAG 2 cut(s) 445, 713
Sse9I AATT 5 cut(s) 60, 139, 157, 294, 679
SspDI GGCGCC 1 cut(s) 268
SspI AATATT 1 cut(s) 538
SspMI CTAG 2 cut(s) 162, 614
SstI GAGCTC 1 cut(s) 586
StyD4I CCNGG 1 cut(s) 588
TaaI ACNGT 2 cut(s) 184, 482
TaiI ACGT 2 cut(s) 156, 494
TaqI TCGA 3 cut(s) 219, 446, 800
TasI AATT 5 cut(s) 60, 139, 157, 294, 679
TatI WGTACW 1 cut(s) 471
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 6 cut(s) 30, 156, 285, 404, 678, 770
Tru9I TTAA 6 cut(s) 30, 156, 285, 404, 678, 770
TscAI CASTG 1 cut(s) 187
TseI GCWGC 2 cut(s) 197, 697
TspDTI ATGAA 4 cut(s) 254, 287, 716, 753
TspGWI ACGGA 2 cut(s) 551, 675
TspRI CASTG 1 cut(s) 187
XagI CCTNNNNNAGG 1 cut(s) 30
XapI RAATTY 1 cut(s) 294
XceI RCATGY 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 723
XspI CTAG 2 cut(s) 162, 614
ZrmI AGTACT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.