Rh2BG475500

Mitochondrial K+-H+ exchange-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011394
Physical Location & Seq
Forward (+)
67055207 .. 67057976
2770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG475500.1

Sequence Viewer

Length: 597 bp
ATGAACAAAGCTTGGATTAGTCTGGAGAAGGCGCCTGAGGGAACCTTTAAAAGAAAGATTCATGGGGTGGGATTGAAGCTATTGGCTCGGGTGAAGCCGTCTGAGATTTTCTTGAAATCTATCACTAACGAGGTCTCACATGTTGAAGTTACATACCCTTCAAGCTTAAATGCACGACTTGTGCGTAGGAGGTTACGACATATTGCCTCGAGGGGATCTATCATACACAAGAAGTACTTATACGGCTCCGTTACGTTGCTCCCTCTGACATCTGCATTTATGGTTTTGCCTTTGCCTAATATTCCCTTCTTCTGGTGTTTATTCCGTACTTACTCTCATTGGCGAGCTCTCCAGGGAAGCGAGAAGCTTCTTCAGCTAGTCTCAGATAGCCCAAAGACTCCAGATGGAAATGGAGTTGAGCATGGAACGAAAAAGTTACTTAATTCTCCGTGGGTTTTGCAGCCATCCAAAGAACTTGAGGAACTTCTTCATCAGGGAGATGAACACAATGATCTGAATAAGCAGGCTATATTAAAAGTCTGCAAAGCCTTTGATTTGAACTCGAATGATGTTCTAAAATACAGAGATACCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mit_KHE1

Protein Analysis

198

Amino Acids

22.6

Weight (kDa)

9.75

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mit_KHE1 PF10173 1 - 127 9.4e-39 Mitochondrial K+-H+ exchange-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53760 AT1G53760
fragaria_vesca FvH4_6g36070 FvH4_6g36070
malus_domestica MD09G1171800.v1.1 MD17G1155200.v1.1
prunus_persica Prupe.3G030200_v2.0.a1
pyrus_communis pycom09g08910 pycom17g14800
rosa_chinensis RchiOBHm_Chr2g0148141
rosa_laevigata RLG00000020320
rosa_multiflora Rmu_sc0001516.1_g000097 Rmu_sc0012028.1_g000004
rosa_roxburghii Rroxscaffold_2G00099050
rosa_rugosa Rorug02G0403900
rosa_samantha Rh2AG462500 Rh2BG475500 Rh2CG449300 Rh2DG484200
rosa_wichuraiana Rw2G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 31
AclWI GGATC 1 cut(s) 223
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 236, 328
AfiI CCNNNNNNNGG 1 cut(s) 312
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 5 cut(s) 76, 115, 146, 162, 559
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 6 cut(s) 11, 79, 165, 347, 367, 376
AluI AGCT 6 cut(s) 11, 79, 165, 347, 367, 376
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 2 cut(s) 139, 385
AlwI GGATC 1 cut(s) 223
Ama87I CYCGRG 2 cut(s) 87, 208
ApeKI GCWGC 1 cut(s) 460
Asp700I GAANNNNTTC 1 cut(s) 486
AspLEI GCGC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 103
AvaI CYCGRG 2 cut(s) 87, 208
AxyI CCTNAGG 1 cut(s) 36
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 349
BarI GAAGNNNNNNTAC 2 cut(s) 224, 256
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 1 cut(s) 472
BccI CCATC 2 cut(s) 398, 472
BceAI ACGGC 2 cut(s) 82, 259
BciT130I CCWGG 1 cut(s) 353
BcoDI GTCTC 2 cut(s) 139, 385
BfaI CTAG 1 cut(s) 377
BfoI RGCGCY 1 cut(s) 35
BisI GCNGC 1 cut(s) 461
BlsI GCNGC 1 cut(s) 462
BmcAI AGTACT 1 cut(s) 236
Bme1390I CCNGG 1 cut(s) 353
BmeT110I CYCGRG 2 cut(s) 87, 208
BmiI GGNNCC 3 cut(s) 33, 43, 247
BmrFI CCNGG 1 cut(s) 353
BpmI CTGGAG 3 cut(s) 44, 335, 384
BpuEI CTTGAG 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 352, 449
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse21I CCTNAGG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 352, 449
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMII CTCAG 3 cut(s) 27, 93, 396
BseXI GCAGC 1 cut(s) 472
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 349
BsiHKCI CYCGRG 2 cut(s) 87, 208
BslI CCNNNNNNNGG 1 cut(s) 312
BsmAI GTCTC 2 cut(s) 139, 385
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 2 cut(s) 87, 208
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 2 cut(s) 215, 511
BspCNI CTCAG 3 cut(s) 28, 94, 395
BspLI GGNNCC 3 cut(s) 33, 43, 247
BspPI GGATC 1 cut(s) 223
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 139
BssECI CCNNGG 2 cut(s) 352, 449
BssMI GATC 2 cut(s) 215, 511
BssNI GRCGYC 1 cut(s) 32
Bst2UI CCWGG 1 cut(s) 353
BstACI GRCGYC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 345, 525
BstDEI CTNAG 3 cut(s) 36, 102, 382
BstDSI CCRYGG 1 cut(s) 449
BstF5I GGATG 1 cut(s) 464
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 2 cut(s) 218, 514
BstMAI GTCTC 2 cut(s) 139, 385
BstMBI GATC 2 cut(s) 215, 511
BstMWI GCNNNNNNNGC 1 cut(s) 373
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 472
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
Bsu36I CCTNAGG 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 449
BtsCI GGATG 1 cut(s) 464
Cac8I GCNNGC 2 cut(s) 345, 525
CfoI GCGC 1 cut(s) 34
Csp6I GTAC 2 cut(s) 235, 327
CviAII CATG 4 cut(s) 62, 140, 422, 592
CviQI GTAC 2 cut(s) 235, 327
DdeI CTNAG 3 cut(s) 36, 102, 382
DinI GGCGCC 1 cut(s) 33
DpnI GATC 2 cut(s) 217, 513
DpnII GATC 2 cut(s) 215, 511
DraI TTTAAA 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 139
Eco53kI GAGCTC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 356
Eco81I CCTNAGG 1 cut(s) 36
Eco88I CYCGRG 2 cut(s) 87, 208
EcoICRI GAGCTC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 349
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
FaeI CATG 4 cut(s) 65, 143, 425, 595
FalI AAGNNNNNCTT 2 cut(s) 221, 253
FatI CATG 4 cut(s) 61, 139, 421, 591
Fnu4HI GCNGC 1 cut(s) 461
FokI GGATG 1 cut(s) 451
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 1 cut(s) 461
FspBI CTAG 1 cut(s) 377
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 461
GsuI CTGGAG 3 cut(s) 44, 335, 384
HaeII RGCGCY 1 cut(s) 35
HhaI GCGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 65, 143, 425, 595
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HindIII AAGCTT 3 cut(s) 9, 163, 365
HinfI GANTC 2 cut(s) 58, 397
HphI GGTGA 1 cut(s) 103
Hpy188I TCNGA 4 cut(s) 103, 267, 385, 516
Hpy188III TCNNGA 3 cut(s) 23, 112, 401
HpyAV CCTTC 3 cut(s) 22, 168, 316
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 4 cut(s) 173, 275, 460, 543
HpyF10VI GCNNNNNNNGC 1 cut(s) 373
HpyF3I CTNAG 3 cut(s) 36, 102, 382
HpySE526I ACGT 1 cut(s) 254
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 65, 143, 425, 595
HspAI GCGC 1 cut(s) 32
KasI GGCGCC 1 cut(s) 31
Kzo9I GATC 2 cut(s) 215, 511
LmnI GCTCC 2 cut(s) 251, 264
LpnPI CCDG 8 cut(s) 8, 48, 298, 338, 365, 414, 479, 509
Lsp1109I GCAGC 1 cut(s) 472
MaeI CTAG 1 cut(s) 377
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 4 cut(s) 148, 192, 250, 435
MalI GATC 2 cut(s) 217, 513
MboI GATC 2 cut(s) 215, 511
MboII GAAGA 3 cut(s) 301, 362, 479
MflI RGATCY 1 cut(s) 215
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 1 cut(s) 442
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 391
MnlI CCTC 7 cut(s) 31, 124, 183, 204, 217, 273, 472
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 4 cut(s) 48, 167, 441, 533
MspR9I CCNGG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 1 cut(s) 373
NarI GGCGCC 1 cut(s) 32
NdeII GATC 2 cut(s) 215, 511
NlaIII CATG 4 cut(s) 65, 143, 425, 595
NlaIV GGNNCC 3 cut(s) 33, 43, 247
NspI RCATGY 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 208
PciI ACATGT 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 1 cut(s) 462
PleI GAGTC 1 cut(s) 391
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 391
PscI ACATGT 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 3 cut(s) 33, 43, 247
PspXI VCTCGAGB 1 cut(s) 208
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 2 cut(s) 236, 328
RsaNI GTAC 2 cut(s) 235, 327
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 4 cut(s) 48, 167, 441, 533
SatI GCNGC 1 cut(s) 461
Sau3AI GATC 2 cut(s) 215, 511
ScaI AGTACT 1 cut(s) 236
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 1 cut(s) 349
SfoI GGCGCC 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 208
SlaI CTCGAG 1 cut(s) 208
SmlI CTYRAG 2 cut(s) 208, 476
SmoI CTYRAG 2 cut(s) 208, 476
Sse9I AATT 1 cut(s) 442
SspDI GGCGCC 1 cut(s) 31
SspI AATATT 1 cut(s) 301
SspMI CTAG 1 cut(s) 377
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 351
TaiI ACGT 1 cut(s) 257
TaqI TCGA 2 cut(s) 209, 563
TasI AATT 1 cut(s) 442
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 4 cut(s) 48, 167, 441, 533
Tru9I TTAA 4 cut(s) 48, 167, 441, 533
TseI GCWGC 1 cut(s) 460
TspDTI ATGAA 4 cut(s) 17, 50, 479, 516
TspGWI ACGGA 3 cut(s) 238, 314, 438
XceI RCATGY 1 cut(s) 143
XhoI CTCGAG 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 486
XspI CTAG 1 cut(s) 377
ZrmI AGTACT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.