Rroxscaffold_2G00099050

Mitochondrial K+-H+ exchange-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011394
Physical Location & Seq
Reverse (-)
20526680 .. 20537777
11098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099050.1

Sequence Viewer

Length: 858 bp
ATGCTCGCAATGTGTGTTGTATCCATATATAAAGCTCGAAAGTTATGCAACGCAAGAGAGATTCAGCAGGTGTTGTTAGCTCCGCATGGCTCCGGCAACTCATCATCGGAATCCGAAGATTTCCGGGATCCGAGCCTCCTCGGCGATCGTTCTGGTACACTTTCTTTGCTTTGCCTTACCCTGTTTCATTCTTTCGCCTATTTGGTTTCGCTTACTCTTGATCAAAGATTGGAGATACTCGTAGTTACTAATACTCCAAAATACAATAAGTGCCATGTCAAGTCTGAGTCATCGCCTTTGGAACCTTTAAAAAGAAAGATTCATGGGGTGGGATTGAAGCTATTGGCTCGGGTGAAGCCGTCTGAGATTTTCTTGAAATCTATCACTAACGAGGTCTCACATGTTGAAGTTACATACCCTTCAAGCTTAAATGCACGACTTGTGCGTAGGAGGTTACGACATATTGCCTCAAGGGGATCTATCATACACAAGAAGTACTTATACGGTTCCGTTACGTTGCTGCCTCTGACATCTGCATTTATGGTTTTGCCTTTGCCTAATATTCCCTTCTTCTGGTGTTTATTCCGCACTTACTCTCATTGGCGAGCTCTCCAGGGAAGCGAGAAGCTTCTTCAGCTAGTCTCAGATAGCCCGAAGACTCCAGATGGAAATGGAGTTGAGCATGGAACAAAAAAGTTACTTAATTCTCCGTGGGTTTTGCAGCCATCCAAAGAACTTGAGGAACTTCTTCATCAGGGAGATGAACACAATGATCTGAATAAGCAGGCTATATTAAAAGTCTGCAAAGCCTTTGATTTGAACTCGAATGATGTTCTAAAATACAGAGATACCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mit_KHE1

Protein Analysis

285

Amino Acids

32.14

Weight (kDa)

9.39

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mit_KHE1 PF10173 85 - 214 2e-35 Mitochondrial K+-H+ exchange-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53760 AT1G53760
fragaria_vesca FvH4_6g36070 FvH4_6g36070
malus_domestica MD09G1171800.v1.1 MD17G1155200.v1.1
prunus_persica Prupe.3G030200_v2.0.a1
pyrus_communis pycom09g08910 pycom17g14800
rosa_chinensis RchiOBHm_Chr2g0148141
rosa_laevigata RLG00000020320
rosa_multiflora Rmu_sc0001516.1_g000097 Rmu_sc0012028.1_g000004
rosa_roxburghii Rroxscaffold_2G00099050
rosa_rugosa Rorug02G0403900
rosa_samantha Rh2AG462500 Rh2BG475500 Rh2CG449300 Rh2DG484200
rosa_wichuraiana Rw2G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 58
Acc36I ACCTGC 1 cut(s) 58
AciI CCGC 2 cut(s) 83, 586
AclWI GGATC 3 cut(s) 122, 135, 484
AcuI CTGAAG 1 cut(s) 617
AfaI GTAC 2 cut(s) 157, 497
AfiI CCNNNNNNNGG 1 cut(s) 573
AflIII ACRYGT 1 cut(s) 400
AgsI TTSAA 5 cut(s) 337, 376, 407, 423, 820
AjnI CCWGG 1 cut(s) 612
AluBI AGCT 7 cut(s) 35, 80, 340, 426, 608, 628, 637
AluI AGCT 7 cut(s) 35, 80, 340, 426, 608, 628, 637
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 2 cut(s) 400, 646
AlwI GGATC 3 cut(s) 122, 135, 484
Ama87I CYCGRG 1 cut(s) 348
ApeKI GCWGC 2 cut(s) 520, 721
Asp700I GAANNNNTTC 1 cut(s) 747
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 364
AvaI CYCGRG 1 cut(s) 348
BamHI GGATCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 610
BarI GAAGNNNNNNTAC 2 cut(s) 485, 517
BbsI GAAGAC 1 cut(s) 662
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 2 cut(s) 507, 733
BccI CCATC 2 cut(s) 659, 733
BceAI ACGGC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 27, 61
BciT130I CCWGG 1 cut(s) 614
BciVI GTATCC 1 cut(s) 31
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 400, 646
BfaI CTAG 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 58
BfuI GTATCC 1 cut(s) 31
BglI GCCNNNNNGGC 1 cut(s) 141
BisI GCNGC 2 cut(s) 521, 722
BlsI GCNGC 2 cut(s) 522, 723
BmcAI AGTACT 1 cut(s) 497
Bme1390I CCNGG 2 cut(s) 125, 614
BmeT110I CYCGRG 1 cut(s) 348
BmiI GGNNCC 4 cut(s) 91, 129, 303, 508
BmrFI CCNGG 2 cut(s) 125, 614
BpiI GAAGAC 1 cut(s) 662
BpmI CTGGAG 2 cut(s) 596, 645
BpuEI CTTGAG 2 cut(s) 454, 758
BpuMI CCSGG 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 400
BsaJI CCNNGG 3 cut(s) 139, 613, 710
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 573
Bse3DI GCAATG 1 cut(s) 15
BseBI CCWGG 1 cut(s) 614
BseDI CCNNGG 3 cut(s) 139, 613, 710
BseGI GGATG 1 cut(s) 725
BseLI CCNNNNNNNGG 1 cut(s) 573
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 3 cut(s) 276, 354, 657
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 2 cut(s) 507, 733
Bsh1285I CGRYCG 1 cut(s) 148
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 1 cut(s) 348
BsiSI CCGG 2 cut(s) 93, 124
BslI CCNNNNNNNGG 1 cut(s) 573
BsmAI GTCTC 2 cut(s) 400, 646
Bso31I GGTCTC 1 cut(s) 400
BsoBI CYCGRG 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 5 cut(s) 127, 145, 220, 476, 772
BspACI CCGC 2 cut(s) 83, 586
BspCNI CTCAG 3 cut(s) 277, 355, 656
BspLI GGNNCC 4 cut(s) 91, 129, 303, 508
BspMI ACCTGC 1 cut(s) 58
BspPI GGATC 3 cut(s) 122, 135, 484
BspTNI GGTCTC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 15
BssECI CCNNGG 3 cut(s) 139, 613, 710
BssMI GATC 5 cut(s) 127, 145, 220, 476, 772
Bst2UI CCWGG 1 cut(s) 614
Bst4CI ACNGT 2 cut(s) 506, 853
BstC8I GCNNGC 3 cut(s) 6, 606, 786
BstDEI CTNAG 3 cut(s) 285, 363, 643
BstDSI CCRYGG 1 cut(s) 710
BstF5I GGATG 1 cut(s) 725
BstKTI GATC 5 cut(s) 130, 148, 223, 479, 775
BstMAI GTCTC 2 cut(s) 400, 646
BstMBI GATC 5 cut(s) 127, 145, 220, 476, 772
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 2 cut(s) 141, 634
BstNI CCWGG 1 cut(s) 614
BstNSI RCATGY 1 cut(s) 404
BstSCI CCNGG 2 cut(s) 123, 612
BstV1I GCAGC 2 cut(s) 507, 733
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 2 cut(s) 127, 476
BstYI RGATCY 2 cut(s) 127, 476
BsuI GTATCC 1 cut(s) 31
BtgI CCRYGG 1 cut(s) 710
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 1 cut(s) 725
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 3 cut(s) 6, 606, 786
Csp6I GTAC 2 cut(s) 156, 496
CviAII CATG 5 cut(s) 86, 275, 323, 401, 683
CviQI GTAC 2 cut(s) 156, 496
DdeI CTNAG 3 cut(s) 285, 363, 643
DpnI GATC 5 cut(s) 129, 147, 222, 478, 774
DpnII GATC 5 cut(s) 127, 145, 220, 476, 772
DraI TTTAAA 1 cut(s) 309
Ecl136II GAGCTC 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 610
Eco31I GGTCTC 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 608
Eco57I CTGAAG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 348
EcoICRI GAGCTC 1 cut(s) 608
EcoRII CCWGG 1 cut(s) 612
EcoT38I GRGCYC 1 cut(s) 610
FaeI CATG 5 cut(s) 89, 278, 326, 404, 686
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FatI CATG 5 cut(s) 85, 274, 322, 400, 682
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 521, 722
FokI GGATG 1 cut(s) 712
FriOI GRGCYC 1 cut(s) 610
Fsp4HI GCNGC 2 cut(s) 521, 722
FspBI CTAG 1 cut(s) 638
GluI GCNGC 2 cut(s) 521, 722
GsuI CTGGAG 2 cut(s) 596, 645
HapII CCGG 2 cut(s) 93, 124
Hin1II CATG 5 cut(s) 89, 278, 326, 404, 686
HindIII AAGCTT 2 cut(s) 424, 626
HinfI GANTC 5 cut(s) 61, 110, 287, 319, 658
HpaII CCGG 2 cut(s) 93, 124
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 8 cut(s) 109, 115, 132, 286, 364, 528, 646, 777
Hpy188III TCNNGA 3 cut(s) 218, 373, 662
Hpy8I GTNNAC 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 429, 577
HpyCH4III ACNGT 2 cut(s) 506, 853
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 5 cut(s) 48, 434, 536, 721, 804
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 634
HpyF3I CTNAG 3 cut(s) 285, 363, 643
HpySE526I ACGT 1 cut(s) 515
Hsp92II CATG 5 cut(s) 89, 278, 326, 404, 686
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 5 cut(s) 127, 145, 220, 476, 772
LmnI GCTCC 2 cut(s) 85, 95
Lsp1109I GCAGC 2 cut(s) 507, 733
MaeI CTAG 1 cut(s) 638
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 5 cut(s) 244, 409, 453, 511, 696
MalI GATC 5 cut(s) 129, 147, 222, 478, 774
MboI GATC 5 cut(s) 127, 145, 220, 476, 772
MboII GAAGA 5 cut(s) 128, 562, 623, 667, 740
MflI RGATCY 2 cut(s) 127, 476
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 1 cut(s) 703
MlyI GAGTC 2 cut(s) 296, 652
MnlI CCTC 7 cut(s) 146, 149, 385, 444, 478, 534, 733
MroXI GAANNNNTTC 1 cut(s) 747
MseI TTAA 4 cut(s) 308, 428, 702, 794
MspI CCGG 2 cut(s) 93, 124
MspR9I CCNGG 2 cut(s) 125, 614
MvaI CCWGG 1 cut(s) 614
MwoI GCNNNNNNNGC 2 cut(s) 141, 634
NciI CCSGG 1 cut(s) 125
NdeII GATC 5 cut(s) 127, 145, 220, 476, 772
NlaIII CATG 5 cut(s) 89, 278, 326, 404, 686
NlaIV GGNNCC 4 cut(s) 91, 129, 303, 508
NmeAIII GCCGAG 1 cut(s) 120
NspI RCATGY 1 cut(s) 404
PaqCI CACCTGC 1 cut(s) 58
PciI ACATGT 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 747
PfeI GAWTC 3 cut(s) 61, 110, 319
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 2 cut(s) 522, 723
Ple19I CGATCG 1 cut(s) 148
PleI GAGTC 2 cut(s) 295, 652
PpsI GAGTC 2 cut(s) 295, 652
PscI ACATGT 1 cut(s) 400
Psp124BI GAGCTC 1 cut(s) 610
Psp6I CCWGG 1 cut(s) 612
PspGI CCWGG 1 cut(s) 612
PspN4I GGNNCC 4 cut(s) 91, 129, 303, 508
PsuI RGATCY 2 cut(s) 127, 476
PvuI CGATCG 1 cut(s) 148
RsaI GTAC 2 cut(s) 157, 497
RsaNI GTAC 2 cut(s) 156, 496
SacI GAGCTC 1 cut(s) 610
SaqAI TTAA 4 cut(s) 308, 428, 702, 794
SatI GCNGC 2 cut(s) 521, 722
Sau3AI GATC 5 cut(s) 127, 145, 220, 476, 772
ScaI AGTACT 1 cut(s) 497
SchI GAGTC 2 cut(s) 296, 652
ScrFI CCNGG 2 cut(s) 125, 614
SduI GDGCHC 1 cut(s) 610
SmlI CTYRAG 2 cut(s) 469, 737
SmoI CTYRAG 2 cut(s) 469, 737
Sse9I AATT 1 cut(s) 703
SsiI CCGC 2 cut(s) 83, 586
SspI AATATT 1 cut(s) 562
SspMI CTAG 1 cut(s) 638
SstI GAGCTC 1 cut(s) 610
StyD4I CCNGG 2 cut(s) 123, 612
TaaI ACNGT 2 cut(s) 506, 853
TaiI ACGT 1 cut(s) 518
TaqI TCGA 2 cut(s) 37, 824
TasI AATT 1 cut(s) 703
TatI WGTACW 1 cut(s) 495
TfiI GAWTC 3 cut(s) 61, 110, 319
Tru1I TTAA 4 cut(s) 308, 428, 702, 794
Tru9I TTAA 4 cut(s) 308, 428, 702, 794
TseI GCWGC 2 cut(s) 520, 721
TspDTI ATGAA 4 cut(s) 176, 311, 740, 777
TspGWI ACGGA 2 cut(s) 499, 699
XceI RCATGY 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 747
XspI CTAG 1 cut(s) 638
ZrmI AGTACT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.