MD17G1155200.v1.1

Mitochondrial K+-H+ exchange-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011394
Physical Location & Seq
Forward (+)
14785150 .. 14788381
3232 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1155200.v1.1.491

Sequence Viewer

Length: 846 bp
ATGAGAGCGAGAGTGATAGTTTTTCCGGTTAGAGGGAGGAACTGGTGCTTCAGCAGATCCGTTGAGCCTCTGGTTTCAGACTCTGCATCTTCCCCTCCATCTCAGACTCCTTCAACTCTCAAAGGCCTGTGGAAGAAGTACAATTCCAATGCCAATGACAAGGCCATTGCCAATCCTTTAGCTGTCAACGCTGAGCTCCTCATCGATTTTGTCTCTACCAAGATGAACATAGCTTGGATCGGTCTGGAGAAGTCGCCTCAGGGAAGTTTTAAGAACAAGATTCATGGATTGGGATTGAAGCTGCTGTCTCGTGTTAAGCCGTCTGAGATTTTCCTAAAATCGATCTCTAATGAGGTCACAGGTGTTGAAGTTAAATACCCTTCCAGCTTAAATGCACAACATGTGCGTAGGAGGTTACGACATATTGCCATGAGGGGATCTATCATACACAGAAAGTATTTATATGGTTCAGTTACGTTGCTTCCTCTGACATCAGCATGTGCTGTTTTGCCTTTGCCTAATATTCCCTTCTTCTGGTGTTTATTCCGTACTTATTCTCATTGGCGAGCTCTCAAGGGAAGTGAGAAGCTCCTTAAGCTAGTCTCAGATAGCTCTGTGGCACCAAGTTCCACTACCGATGGAAATAAAAAAGAGCATATTGACTCTCAGCGTGGAAGCAAAGAGATATTCAATTCTCCTTATGTTTTGCAGCCATCGAAAGAGCTTGAGGAGCTTCTTCGTCATGGAGGCGAGCGCGAGGGTCTCAAAGAATGTGCTATATTGGATATCTGCAAAATCTATGATTTGAACACACATGACGTTCTAAAGTACCGGGACTCGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mit_KHE1

Protein Analysis

282

Amino Acids

31.6

Weight (kDa)

9.72

Isoelectric Point (pI)

53.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mit_KHE1 PF10173 4 - 201 2.3e-41 Mitochondrial K+-H+ exchange-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53760 AT1G53760
fragaria_vesca FvH4_6g36070 FvH4_6g36070
malus_domestica MD09G1171800.v1.1 MD17G1155200.v1.1
prunus_persica Prupe.3G030200_v2.0.a1
pyrus_communis pycom09g08910 pycom17g14800
rosa_chinensis RchiOBHm_Chr2g0148141
rosa_laevigata RLG00000020320
rosa_multiflora Rmu_sc0001516.1_g000097 Rmu_sc0012028.1_g000004
rosa_roxburghii Rroxscaffold_2G00099050
rosa_rugosa Rorug02G0403900
rosa_samantha Rh2AG462500 Rh2BG475500 Rh2CG449300 Rh2DG484200
rosa_wichuraiana Rw2G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 619
AccII CGCG 1 cut(s) 756
AclWI GGATC 3 cut(s) 51, 245, 445
AcuI CTGAAG 1 cut(s) 34
AfaI GTAC 3 cut(s) 140, 550, 830
AfiI CCNNNNNNNGG 2 cut(s) 32, 534
AflII CTTAAG 1 cut(s) 593
AflIII ACRYGT 1 cut(s) 400
AgsI TTSAA 5 cut(s) 114, 298, 368, 691, 808
Alw21I GWGCWC 2 cut(s) 198, 571
Alw26I GTCTC 4 cut(s) 217, 312, 607, 767
AlwI GGATC 3 cut(s) 51, 245, 445
AlwNI CAGNNNCTG 1 cut(s) 83
AoxI GGCC 2 cut(s) 124, 162
ApeKI GCWGC 2 cut(s) 301, 709
AspLEI GCGC 1 cut(s) 756
AsuC2I CCSGG 1 cut(s) 833
AxyI CCTNAGG 1 cut(s) 258
BanI GGYRCC 1 cut(s) 619
BanII GRGCYC 2 cut(s) 198, 571
BauI CACGAG 1 cut(s) 309
Bbv12I GWGCWC 2 cut(s) 198, 571
BbvI GCAGC 2 cut(s) 288, 721
BccI CCATC 3 cut(s) 106, 632, 721
BceAI ACGGC 1 cut(s) 304
BcnI CCSGG 1 cut(s) 833
BcoDI GTCTC 4 cut(s) 217, 312, 607, 767
BfaI CTAG 1 cut(s) 599
BfrI CTTAAG 1 cut(s) 593
BisI GCNGC 2 cut(s) 302, 710
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 2 cut(s) 303, 711
Bme1390I CCNGG 1 cut(s) 833
BmiI GGNNCC 1 cut(s) 621
BmrFI CCNGG 1 cut(s) 833
BmsI GCATC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 266
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 2 cut(s) 557, 746
BpuMI CCSGG 1 cut(s) 833
Bsa29I ATCGAT 2 cut(s) 204, 341
BsaI GGTCTC 1 cut(s) 767
BsaWI WCCGGW 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 534
Bse1I ACTGG 1 cut(s) 47
Bse21I CCTNAGG 1 cut(s) 258
Bse3DI GCAATG 1 cut(s) 165
BseCI ATCGAT 2 cut(s) 204, 341
BseLI CCNNNNNNNGG 2 cut(s) 32, 534
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 6 cut(s) 116, 183, 272, 315, 618, 680
BseNI ACTGG 1 cut(s) 47
BseRI GAGGAG 2 cut(s) 188, 743
BseXI GCAGC 2 cut(s) 288, 721
Bsh1236I CGCG 1 cut(s) 756
BshFI GGCC 2 cut(s) 126, 164
BshNI GGYRCC 1 cut(s) 619
BshVI ATCGAT 2 cut(s) 204, 341
BsiHKAI GWGCWC 2 cut(s) 198, 571
BsiSI CCGG 2 cut(s) 26, 832
BslI CCNNNNNNNGG 2 cut(s) 32, 534
BsmAI GTCTC 4 cut(s) 217, 312, 607, 767
BsnI GGCC 2 cut(s) 126, 164
Bso31I GGTCTC 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 198, 571
Bsp143I GATC 4 cut(s) 56, 237, 342, 437
Bsp1720I GCTNAGC 1 cut(s) 192
BspANI GGCC 2 cut(s) 126, 164
BspCNI CTCAG 6 cut(s) 115, 184, 271, 316, 617, 679
BspDI ATCGAT 2 cut(s) 204, 341
BspFNI CGCG 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 621
BspPI GGATC 3 cut(s) 51, 245, 445
BspT107I GGYRCC 1 cut(s) 619
BspTI CTTAAG 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 767
BsrDI GCAATG 1 cut(s) 165
BsrI ACTGG 1 cut(s) 47
BssMI GATC 4 cut(s) 56, 237, 342, 437
BssSI CACGAG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 309
BstAFI CTTAAG 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 567, 752
BstDEI CTNAG 6 cut(s) 102, 192, 258, 324, 604, 666
BstFNI CGCG 1 cut(s) 756
BstHHI GCGC 1 cut(s) 756
BstKTI GATC 4 cut(s) 59, 240, 345, 440
BstMAI GTCTC 4 cut(s) 217, 312, 607, 767
BstMBI GATC 4 cut(s) 56, 237, 342, 437
BstMWI GCNNNNNNNGC 3 cut(s) 188, 595, 730
BstNSI RCATGY 2 cut(s) 404, 501
BstSCI CCNGG 1 cut(s) 831
BstUI CGCG 1 cut(s) 756
BstV1I GCAGC 2 cut(s) 288, 721
BstX2I RGATCY 2 cut(s) 56, 437
BstYI RGATCY 2 cut(s) 56, 437
Bsu15I ATCGAT 2 cut(s) 204, 341
Bsu36I CCTNAGG 1 cut(s) 258
BsuRI GGCC 2 cut(s) 126, 164
BsuTUI ATCGAT 2 cut(s) 204, 341
Cac8I GCNNGC 2 cut(s) 567, 752
CaiI CAGNNNCTG 1 cut(s) 83
CfoI GCGC 1 cut(s) 756
ClaI ATCGAT 2 cut(s) 204, 341
Csp6I GTAC 3 cut(s) 139, 549, 829
CviAII CATG 6 cut(s) 284, 401, 430, 498, 743, 815
CviQI GTAC 3 cut(s) 139, 549, 829
DdeI CTNAG 6 cut(s) 102, 192, 258, 324, 604, 666
DpnI GATC 4 cut(s) 58, 239, 344, 439
DpnII GATC 4 cut(s) 56, 237, 342, 437
Ecl136II GAGCTC 2 cut(s) 196, 569
Eco147I AGGCCT 1 cut(s) 126
Eco24I GRGCYC 2 cut(s) 198, 571
Eco31I GGTCTC 1 cut(s) 767
Eco32I GATATC 1 cut(s) 787
Eco53kI GAGCTC 2 cut(s) 196, 569
Eco57I CTGAAG 1 cut(s) 34
Eco81I CCTNAGG 1 cut(s) 258
EcoICRI GAGCTC 2 cut(s) 196, 569
EcoRV GATATC 1 cut(s) 787
EcoT38I GRGCYC 2 cut(s) 198, 571
FaeI CATG 6 cut(s) 287, 404, 433, 501, 746, 818
FatI CATG 6 cut(s) 283, 400, 429, 497, 742, 814
Fnu4HI GCNGC 2 cut(s) 302, 710
FriOI GRGCYC 2 cut(s) 198, 571
Fsp4HI GCNGC 2 cut(s) 302, 710
FspBI CTAG 1 cut(s) 599
GlaI GCGC 1 cut(s) 755
GluI GCNGC 2 cut(s) 302, 710
GsuI CTGGAG 1 cut(s) 266
HaeIII GGCC 2 cut(s) 126, 164
HapII CCGG 2 cut(s) 26, 832
HhaI GCGC 1 cut(s) 756
Hin1II CATG 6 cut(s) 287, 404, 433, 501, 746, 818
Hin6I GCGC 1 cut(s) 754
HinP1I GCGC 1 cut(s) 754
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 5 cut(s) 80, 106, 280, 662, 836
HpaII CCGG 2 cut(s) 26, 832
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 5 cut(s) 79, 105, 325, 489, 607
Hpy188III TCNNGA 1 cut(s) 245
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 3 cut(s) 120, 390, 538
HpyCH4IV ACGT 2 cut(s) 476, 819
HpyCH4V TGCA 4 cut(s) 86, 395, 709, 792
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 595, 730
HpyF3I CTNAG 6 cut(s) 102, 192, 258, 324, 604, 666
HpySE526I ACGT 2 cut(s) 476, 819
Hsp92II CATG 6 cut(s) 287, 404, 433, 501, 746, 818
HspAI GCGC 1 cut(s) 754
Kzo9I GATC 4 cut(s) 56, 237, 342, 437
LmnI GCTCC 3 cut(s) 201, 594, 730
LpnPI CCDG 9 cut(s) 28, 39, 56, 140, 230, 245, 345, 397, 520
Lsp1109I GCAGC 2 cut(s) 288, 721
LweI GCATC 1 cut(s) 95
MaeI CTAG 1 cut(s) 599
MaeII ACGT 2 cut(s) 476, 819
MaeIII GTNAC 3 cut(s) 355, 414, 472
MalI GATC 4 cut(s) 58, 239, 344, 439
MboI GATC 4 cut(s) 56, 237, 342, 437
MboII GAAGA 4 cut(s) 81, 145, 523, 728
MflI RGATCY 2 cut(s) 56, 437
MhlI GDGCHC 2 cut(s) 198, 571
MluCI AATT 2 cut(s) 142, 691
MlyI GAGTC 4 cut(s) 74, 100, 656, 830
MseI TTAA 5 cut(s) 270, 315, 372, 389, 594
MslI CAYNNNNRTG 1 cut(s) 496
MspCI CTTAAG 1 cut(s) 593
MspI CCGG 2 cut(s) 26, 832
MspR9I CCNGG 1 cut(s) 833
MvnI CGCG 1 cut(s) 756
MwoI GCNNNNNNNGC 3 cut(s) 188, 595, 730
NciI CCSGG 1 cut(s) 833
NdeII GATC 4 cut(s) 56, 237, 342, 437
NlaIII CATG 6 cut(s) 287, 404, 433, 501, 746, 818
NlaIV GGNNCC 1 cut(s) 621
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 2 cut(s) 404, 501
PceI AGGCCT 1 cut(s) 126
PciI ACATGT 1 cut(s) 400
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 2 cut(s) 303, 711
PleI GAGTC 4 cut(s) 74, 100, 656, 830
PpsI GAGTC 4 cut(s) 74, 100, 656, 830
PscI ACATGT 1 cut(s) 400
Psp124BI GAGCTC 2 cut(s) 198, 571
PspN4I GGNNCC 1 cut(s) 621
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 2 cut(s) 56, 437
RsaI GTAC 3 cut(s) 140, 550, 830
RsaNI GTAC 3 cut(s) 139, 549, 829
RseI CAYNNNNRTG 1 cut(s) 496
SacI GAGCTC 2 cut(s) 198, 571
SaqAI TTAA 5 cut(s) 270, 315, 372, 389, 594
SatI GCNGC 2 cut(s) 302, 710
Sau3AI GATC 4 cut(s) 56, 237, 342, 437
SchI GAGTC 4 cut(s) 74, 100, 656, 830
ScrFI CCNGG 1 cut(s) 833
SduI GDGCHC 2 cut(s) 198, 571
SfaNI GCATC 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 496
SmlI CTYRAG 3 cut(s) 572, 593, 725
SmoI CTYRAG 3 cut(s) 572, 593, 725
Sse9I AATT 2 cut(s) 142, 691
SseBI AGGCCT 1 cut(s) 126
SspI AATATT 1 cut(s) 523
SspMI CTAG 1 cut(s) 599
SstI GAGCTC 2 cut(s) 198, 571
StuI AGGCCT 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 831
TaiI ACGT 2 cut(s) 479, 822
TaqI TCGA 4 cut(s) 204, 341, 716, 839
TaqII GACCGA 1 cut(s) 230
TasI AATT 2 cut(s) 142, 691
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 5 cut(s) 270, 315, 372, 389, 594
Tru9I TTAA 5 cut(s) 270, 315, 372, 389, 594
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 2 cut(s) 301, 709
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 2 cut(s) 239, 272
TspGWI ACGGA 2 cut(s) 49, 536
Vha464I CTTAAG 1 cut(s) 593
XceI RCATGY 2 cut(s) 404, 501
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.