MD09G1171800.v1.1

Mitochondrial K+-H+ exchange-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011394
Physical Location & Seq
Reverse (-)
14517057 .. 14520505
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1171800.v1.1.491

Sequence Viewer

Length: 846 bp
ATGAGAGCGAGAGTGATAGTTTTTCCGGTTAAAGGGAGGAACTGGTGCTTCAGCAGATCCATTGAGCCTCTGGTTTCAGACTCTGCCTCTGCCTCTGCCTCTCAAACTCCTTCAACTCTCAAAGACCTCTGGAAAAAGTACAAGTCCAATGCCAATGACAAGGCCATTACCAATCCCTTAGCTGCCAACGCTGAGCTCCTCATCGATTTCGTCTCTACCAAGATGAACAGAGCTTGGATCGGTCTGGAGAAGTCGCCTCAGGGAAGTTTTAAGAACAAGCTTCATGGATTGGGATTGAAGCTGTTGGCTCGTGTTAAGCCGTCTGAGATTTTCCTGAAATCGATCTCTACTGAGGTCACAGGTGTTGAAGTTAAATACCCGTCCAGCTTAAATGCACGACTTGTACGTAGGAGGCTACGGCATATTGCCATGAGGGGATCTATCATACACAAAAAGTTTTTATATGGTTCAGTTACGTTGCTTCCTCTGACATCTGCCTTTACCGTTTTGCCTCTGCCTAATATTCCCTTCTTCTGGTGTTTATTCCGTACTTATTCTCATTGGCGAGCTCTCAAGGGAAGTGAGAAGCTCCTTCAGCTAGTCTCAGATAGCTCTGTGACACCAAGTTCCACTACTGATGGAAATGAAAATGAGCATATTGACTCTCAGCATGGAAGCAAAGAGATATTCGATTCTCCTTACGTTTTGCAGCCATCAAAAGAGCTTGAGGAACTTCTTTGTTATGGAGGCGAGCGTGAGGGTCTCAAAGAATGTGCGATATTAGATATCTGCAAAACCTTCGATTTGAACACGAATGATGTTTTAAAGTACAGGGGCTCGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mit_KHE1

Protein Analysis

282

Amino Acids

31.49

Weight (kDa)

9.5

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mit_KHE1 PF10173 4 - 201 7.1e-42 Mitochondrial K+-H+ exchange-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53760 AT1G53760
fragaria_vesca FvH4_6g36070 FvH4_6g36070
malus_domestica MD09G1171800.v1.1 MD17G1155200.v1.1
prunus_persica Prupe.3G030200_v2.0.a1
pyrus_communis pycom09g08910 pycom17g14800
rosa_chinensis RchiOBHm_Chr2g0148141
rosa_laevigata RLG00000020320
rosa_multiflora Rmu_sc0001516.1_g000097 Rmu_sc0012028.1_g000004
rosa_roxburghii Rroxscaffold_2G00099050
rosa_rugosa Rorug02G0403900
rosa_samantha Rh2AG462500 Rh2BG475500 Rh2CG449300 Rh2DG484200
rosa_wichuraiana Rw2G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 51, 245, 445
AcuI CTGAAG 2 cut(s) 34, 578
AfaI GTAC 4 cut(s) 140, 405, 550, 830
AfiI CCNNNNNNNGG 2 cut(s) 32, 534
AgsI TTSAA 4 cut(s) 114, 298, 368, 808
Alw21I GWGCWC 2 cut(s) 198, 571
Alw26I GTCTC 3 cut(s) 217, 607, 767
AlwI GGATC 3 cut(s) 51, 245, 445
AlwNI CAGNNNCTG 1 cut(s) 83
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 182, 709
AxyI CCTNAGG 1 cut(s) 258
BanII GRGCYC 3 cut(s) 198, 571, 839
BauI CACGAG 1 cut(s) 309
Bbv12I GWGCWC 2 cut(s) 198, 571
BbvI GCAGC 2 cut(s) 169, 721
BccI CCATC 2 cut(s) 632, 721
BceAI ACGGC 2 cut(s) 304, 434
BcgI CGANNNNNNTGC 2 cut(s) 781, 815
BcoDI GTCTC 3 cut(s) 217, 607, 767
BfaI CTAG 1 cut(s) 599
BisI GCNGC 2 cut(s) 183, 710
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 2 cut(s) 184, 711
BpmI CTGGAG 1 cut(s) 266
Bpu10I CCTNAGC 1 cut(s) 178
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 2 cut(s) 557, 746
Bsa29I ATCGAT 2 cut(s) 204, 341
BsaAI YACGTR 1 cut(s) 407
BsaI GGTCTC 1 cut(s) 767
BsaWI WCCGGW 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 534
Bse1I ACTGG 1 cut(s) 47
Bse21I CCTNAGG 1 cut(s) 258
BseCI ATCGAT 2 cut(s) 204, 341
BseLI CCNNNNNNNGG 2 cut(s) 32, 534
BseMII CTCAG 6 cut(s) 183, 272, 315, 342, 618, 680
BseNI ACTGG 1 cut(s) 47
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 2 cut(s) 169, 721
BshFI GGCC 1 cut(s) 164
BshVI ATCGAT 2 cut(s) 204, 341
BsiHKAI GWGCWC 2 cut(s) 198, 571
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 2 cut(s) 32, 534
BsmAI GTCTC 3 cut(s) 217, 607, 767
BsmBI CGTCTC 1 cut(s) 217
BsnI GGCC 1 cut(s) 164
Bso31I GGTCTC 1 cut(s) 767
Bsp1286I GDGCHC 3 cut(s) 198, 571, 839
Bsp143I GATC 4 cut(s) 56, 237, 342, 437
Bsp1720I GCTNAGC 1 cut(s) 192
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 6 cut(s) 184, 271, 316, 343, 617, 679
BspDI ATCGAT 2 cut(s) 204, 341
BspPI GGATC 3 cut(s) 51, 245, 445
BspTNI GGTCTC 1 cut(s) 767
BsrI ACTGG 1 cut(s) 47
BssMI GATC 4 cut(s) 56, 237, 342, 437
BssSI CACGAG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 505
BstBAI YACGTR 1 cut(s) 407
BstC8I GCNNGC 2 cut(s) 567, 752
BstDEI CTNAG 7 cut(s) 178, 192, 258, 324, 351, 604, 666
BstKTI GATC 4 cut(s) 59, 240, 345, 440
BstMAI GTCTC 3 cut(s) 217, 607, 767
BstMBI GATC 4 cut(s) 56, 237, 342, 437
BstMWI GCNNNNNNNGC 2 cut(s) 188, 595
BstSNI TACGTA 1 cut(s) 407
BstV1I GCAGC 2 cut(s) 169, 721
BstX2I RGATCY 2 cut(s) 56, 437
BstYI RGATCY 2 cut(s) 56, 437
Bsu15I ATCGAT 2 cut(s) 204, 341
Bsu36I CCTNAGG 1 cut(s) 258
BsuRI GGCC 1 cut(s) 164
BsuTUI ATCGAT 2 cut(s) 204, 341
Cac8I GCNNGC 2 cut(s) 567, 752
CaiI CAGNNNCTG 1 cut(s) 83
ClaI ATCGAT 2 cut(s) 204, 341
Csp6I GTAC 4 cut(s) 139, 404, 549, 829
CviAII CATG 3 cut(s) 284, 430, 671
CviQI GTAC 4 cut(s) 139, 404, 549, 829
DdeI CTNAG 7 cut(s) 178, 192, 258, 324, 351, 604, 666
DpnI GATC 4 cut(s) 58, 239, 344, 439
DpnII GATC 4 cut(s) 56, 237, 342, 437
DraI TTTAAA 1 cut(s) 825
Ecl136II GAGCTC 2 cut(s) 196, 569
Eco105I TACGTA 1 cut(s) 407
Eco24I GRGCYC 3 cut(s) 198, 571, 839
Eco31I GGTCTC 1 cut(s) 767
Eco32I GATATC 1 cut(s) 787
Eco53kI GAGCTC 2 cut(s) 196, 569
Eco57I CTGAAG 2 cut(s) 34, 578
Eco81I CCTNAGG 1 cut(s) 258
EcoICRI GAGCTC 2 cut(s) 196, 569
EcoRV GATATC 1 cut(s) 787
EcoT38I GRGCYC 3 cut(s) 198, 571, 839
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 3 cut(s) 287, 433, 674
FaiI YATR 9 cut(s) 285, 423, 431, 446, 463, 465, 657, 672, 744
FatI CATG 3 cut(s) 283, 429, 670
Fnu4HI GCNGC 2 cut(s) 183, 710
FriOI GRGCYC 3 cut(s) 198, 571, 839
Fsp4HI GCNGC 2 cut(s) 183, 710
FspBI CTAG 1 cut(s) 599
GluI GCNGC 2 cut(s) 183, 710
GsuI CTGGAG 1 cut(s) 266
HaeIII GGCC 1 cut(s) 164
HapII CCGG 1 cut(s) 26
Hin1II CATG 3 cut(s) 287, 433, 674
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 3 cut(s) 80, 662, 692
HpaII CCGG 1 cut(s) 26
Hpy188I TCNGA 4 cut(s) 79, 325, 489, 607
Hpy188III TCNNGA 3 cut(s) 130, 245, 334
Hpy99I CGWCG 1 cut(s) 844
HpyAV CCTTC 4 cut(s) 120, 538, 602, 808
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 4 cut(s) 406, 476, 702, 842
HpyCH4V TGCA 3 cut(s) 395, 709, 792
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 595
HpyF3I CTNAG 7 cut(s) 178, 192, 258, 324, 351, 604, 666
HpySE526I ACGT 4 cut(s) 406, 476, 702, 842
Hsp92II CATG 3 cut(s) 287, 433, 674
Kzo9I GATC 4 cut(s) 56, 237, 342, 437
LmnI GCTCC 2 cut(s) 201, 594
Lsp1109I GCAGC 2 cut(s) 169, 721
MaeI CTAG 1 cut(s) 599
MaeII ACGT 4 cut(s) 406, 476, 702, 842
MaeIII GTNAC 3 cut(s) 355, 472, 616
MalI GATC 4 cut(s) 58, 239, 344, 439
MboI GATC 4 cut(s) 56, 237, 342, 437
MboII GAAGA 1 cut(s) 523
MflI RGATCY 2 cut(s) 56, 437
MhlI GDGCHC 3 cut(s) 198, 571, 839
MlyI GAGTC 2 cut(s) 74, 656
MseI TTAA 6 cut(s) 30, 270, 315, 372, 389, 824
MspI CCGG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 188, 595
NdeII GATC 4 cut(s) 56, 237, 342, 437
NlaIII CATG 3 cut(s) 287, 433, 674
NmuCI GTSAC 2 cut(s) 355, 616
PcsI WCGNNNNNNNCGW 1 cut(s) 403
PfeI GAWTC 1 cut(s) 692
PkrI GCNGC 2 cut(s) 184, 711
PleI GAGTC 2 cut(s) 74, 656
PpsI GAGTC 2 cut(s) 74, 656
Ppu21I YACGTR 1 cut(s) 407
Psp124BI GAGCTC 2 cut(s) 198, 571
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 2 cut(s) 56, 437
RsaI GTAC 4 cut(s) 140, 405, 550, 830
RsaNI GTAC 4 cut(s) 139, 404, 549, 829
SacI GAGCTC 2 cut(s) 198, 571
SaqAI TTAA 6 cut(s) 30, 270, 315, 372, 389, 824
SatI GCNGC 2 cut(s) 183, 710
Sau3AI GATC 4 cut(s) 56, 237, 342, 437
SchI GAGTC 2 cut(s) 74, 656
SduI GDGCHC 3 cut(s) 198, 571, 839
SmlI CTYRAG 2 cut(s) 572, 725
SmoI CTYRAG 2 cut(s) 572, 725
SnaBI TACGTA 1 cut(s) 407
SspI AATATT 1 cut(s) 523
SspMI CTAG 1 cut(s) 599
SstI GAGCTC 2 cut(s) 198, 571
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 4 cut(s) 409, 479, 705, 845
TaqI TCGA 5 cut(s) 204, 341, 690, 801, 839
TaqII GACCGA 1 cut(s) 230
TatI WGTACW 2 cut(s) 138, 828
TfiI GAWTC 1 cut(s) 692
Tru1I TTAA 6 cut(s) 30, 270, 315, 372, 389, 824
Tru9I TTAA 6 cut(s) 30, 270, 315, 372, 389, 824
TseFI GTSAC 2 cut(s) 355, 616
TseI GCWGC 2 cut(s) 182, 709
Tsp45I GTSAC 2 cut(s) 355, 616
TspDTI ATGAA 3 cut(s) 239, 272, 660
TspGWI ACGGA 1 cut(s) 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.