AT1G55140

Ribonuclease III family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003592
Physical Location & Seq
Reverse (-)
20572405 .. 20574382
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G55140.1

Sequence Viewer

Length: 714 bp
ATGGCGATTCTATCAGCGAGCTCTACTATAGTGAGAGCTGCTTTAGATACGCAGCCGAAGCTCCGATACAATCCCAATGCGCCACGCAATGTTAAGAAAAATTCTAATTTGAGTTCTTTTGTTCCACCGTCGTCTCCTTCTTCATCTTCTCCGGCGACAAACTTAAGCGTCTCCGTTTCTGATTTACTCAAACGTCCTGCAAGCAAAGATATTGGTGATGGATTTGATGATACTTGTGTTGGATATGAGAAATGGTTTCCAAGTCCACCAAAAGTGGAGAAACCTAGATCTGTCTTCAATGCTGCCTCATTAGCTTATATTGGCGATTCAATCTATGAGATATATGCTCGTAGGCATTTTCTTTTTCCTCCACTTAGTATTGAAGAATATAATGACCGTGTTAGAGCAGTGGTGCGATGTGAAGCACAATATGCTTTGCTGCAGAAGCTTGTTGATGATGACTTCTTAACGAAAGATGAAAGAGATATACTTCGGTGGGGAAAAAACGTAGGCTCGGTTAAAACACGGTCAACTAGACGTGCCGGTGTTGCTGTTTATAACAAGGCCTCATCATTGGAAACACTAATTGGGTATTTGTATCTATCAAACGGGAAAAGACTGGAAGAAATGATGCAGAAGCTAGGATTCTCAAGTGGTTCTTCAACAGAGAGAATGGTTAAGGAAGCTGGCTCAAACAAACCATCTTTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

237

Amino Acids

26.3

Weight (kDa)

9.55

Isoelectric Point (pI)

52.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 103 - 204 1.3e-21 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
AcsI RAATTY 1 cut(s) 100
AflII CTTAAG 1 cut(s) 163
AgsI TTSAA 5 cut(s) 298, 330, 383, 663, 709
AjiI CACGTC 1 cut(s) 539
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
AluBI AGCT 7 cut(s) 21, 38, 61, 314, 448, 640, 686
AluI AGCT 7 cut(s) 21, 38, 61, 314, 448, 640, 686
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 2 cut(s) 138, 175
AoxI GGCC 1 cut(s) 564
ApeKI GCWGC 4 cut(s) 38, 52, 302, 439
ApoI RAATTY 1 cut(s) 100
AspLEI GCGC 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 227
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BanII GRGCYC 1 cut(s) 23
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 4 cut(s) 25, 64, 289, 426
BccI CCATC 2 cut(s) 212, 709
BcoDI GTCTC 2 cut(s) 138, 175
BfaI CTAG 3 cut(s) 285, 534, 641
BfmI CTRYAG 2 cut(s) 27, 440
BfrI CTTAAG 1 cut(s) 163
BglII AGATCT 1 cut(s) 287
BisI GCNGC 4 cut(s) 39, 53, 303, 440
BlsI GCNGC 4 cut(s) 40, 54, 304, 441
BmgBI CACGTC 1 cut(s) 539
BmsI GCATC 1 cut(s) 621
BpiI GAAGAC 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 634
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 1 cut(s) 624
Bse3DI GCAATG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 624
BseXI GCAGC 4 cut(s) 25, 64, 289, 426
BshFI GGCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 2 cut(s) 152, 543
BsmAI GTCTC 2 cut(s) 138, 175
BsmBI CGTCTC 2 cut(s) 138, 175
BsnI GGCC 1 cut(s) 566
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 1 cut(s) 287
BspANI GGCC 1 cut(s) 566
BspMAI CTGCAG 1 cut(s) 444
BspTI CTTAAG 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 1 cut(s) 624
BssAI RCCGGY 1 cut(s) 542
BssMI GATC 1 cut(s) 287
Bst4CI ACNGT 3 cut(s) 129, 398, 528
BstAFI CTTAAG 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 431
BstC8I GCNNGC 3 cut(s) 19, 202, 688
BstDEI CTNAG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 1 cut(s) 290
BstMAI GTCTC 2 cut(s) 138, 175
BstMBI GATC 1 cut(s) 287
BstMWI GCNNNNNNNGC 5 cut(s) 58, 311, 431, 445, 548
BstSFI CTRYAG 2 cut(s) 27, 440
BstV1I GCAGC 4 cut(s) 25, 64, 289, 426
BstV2I GAAGAC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BsuRI GGCC 1 cut(s) 566
BtgZI GCGATG 1 cut(s) 430
BtrI CACGTC 1 cut(s) 539
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 3 cut(s) 19, 202, 688
CfoI GCGC 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 542
CseI GACGC 1 cut(s) 157
DdeI CTNAG 1 cut(s) 374
DpnI GATC 1 cut(s) 289
DpnII GATC 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 566
Eco24I GRGCYC 1 cut(s) 23
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 23
Esp3I CGTCTC 2 cut(s) 138, 175
FalI AAGNNNNNCTT 2 cut(s) 643, 675
Fnu4HI GCNGC 4 cut(s) 39, 53, 303, 440
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 4 cut(s) 39, 53, 303, 440
FspBI CTAG 3 cut(s) 285, 534, 641
GlaI GCGC 1 cut(s) 81
GluI GCNGC 4 cut(s) 39, 53, 303, 440
HaeIII GGCC 1 cut(s) 566
HapII CCGG 2 cut(s) 152, 543
HgaI GACGC 1 cut(s) 157
HhaI GCGC 1 cut(s) 82
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 531
HindII GTYRAC 1 cut(s) 531
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 3 cut(s) 7, 326, 645
HpaII CCGG 2 cut(s) 152, 543
HphI GGTGA 1 cut(s) 227
Hpy166II GTNNAC 2 cut(s) 266, 531
Hpy188I TCNGA 2 cut(s) 65, 181
Hpy8I GTNNAC 2 cut(s) 266, 531
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 1 cut(s) 147
HpyCH4III ACNGT 3 cut(s) 129, 398, 528
HpyCH4IV ACGT 3 cut(s) 193, 507, 538
HpyCH4V TGCA 3 cut(s) 200, 442, 634
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 311, 431, 445, 548
HpyF3I CTNAG 1 cut(s) 374
HpySE526I ACGT 3 cut(s) 193, 507, 538
HspAI GCGC 1 cut(s) 80
Kzo9I GATC 1 cut(s) 287
LmnI GCTCC 1 cut(s) 66
LpnPI CCDG 5 cut(s) 165, 210, 556, 605, 672
Lsp1109I GCAGC 4 cut(s) 25, 64, 289, 426
LweI GCATC 1 cut(s) 621
MaeI CTAG 3 cut(s) 285, 534, 641
MaeII ACGT 3 cut(s) 193, 507, 538
MalI GATC 1 cut(s) 289
MboI GATC 1 cut(s) 287
MboII GAAGA 6 cut(s) 132, 138, 286, 395, 635, 651
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 3 cut(s) 100, 106, 585
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 3 cut(s) 316, 378, 577
MseI TTAA 5 cut(s) 93, 164, 467, 519, 678
MspCI CTTAAG 1 cut(s) 163
MspI CCGG 2 cut(s) 152, 543
MwoI GCNNNNNNNGC 5 cut(s) 58, 311, 431, 445, 548
NdeII GATC 1 cut(s) 287
PceI AGGCCT 1 cut(s) 566
PfeI GAWTC 3 cut(s) 7, 326, 645
PkrI GCNGC 4 cut(s) 40, 54, 304, 441
PsiI TTATAA 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 23
PstI CTGCAG 1 cut(s) 444
PsuI RGATCY 1 cut(s) 287
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 5 cut(s) 93, 164, 467, 519, 678
SatI GCNGC 4 cut(s) 39, 53, 303, 440
Sau3AI GATC 1 cut(s) 287
SduI GDGCHC 1 cut(s) 23
SfaNI GCATC 1 cut(s) 621
SfcI CTRYAG 2 cut(s) 27, 440
SmlI CTYRAG 2 cut(s) 163, 649
SmoI CTYRAG 2 cut(s) 163, 649
Sse9I AATT 3 cut(s) 100, 106, 585
SseBI AGGCCT 1 cut(s) 566
SspMI CTAG 3 cut(s) 285, 534, 641
SstI GAGCTC 1 cut(s) 23
StuI AGGCCT 1 cut(s) 566
TaaI ACNGT 3 cut(s) 129, 398, 528
TaiI ACGT 3 cut(s) 196, 510, 541
TasI AATT 3 cut(s) 100, 106, 585
TfiI GAWTC 3 cut(s) 7, 326, 645
Tru1I TTAA 5 cut(s) 93, 164, 467, 519, 678
Tru9I TTAA 5 cut(s) 93, 164, 467, 519, 678
TscAI CASTG 1 cut(s) 414
TseI GCWGC 4 cut(s) 38, 52, 302, 439
TspDTI ATGAA 2 cut(s) 132, 492
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 1 cut(s) 414
Vha464I CTTAAG 1 cut(s) 163
XapI RAATTY 1 cut(s) 100
XspI CTAG 3 cut(s) 285, 534, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.