FvH4_4g12940

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003592
Physical Location & Seq
Reverse (-)
16587054 .. 16591818
4765 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12940.t2

Sequence Viewer

Length: 750 bp
ATGCATGCTCTATCACCAATGAGTGTGAGGGTAGTGAGAGTGGGAGCTTCAGTGGATACGCAACAAAGACTCCCGTACAACCGCAACGCCCCAAGAAAGATCAAGGACAGCTCCAGCACTCTGTCTACCACCCAAAACCCTCCAACTGTCACTCTGACCGCCTCAACTACTCGTACGGTTGACCATGTTTTTGAGCTTCTCAAACGCCCAAATCAAGGAATGGATGACTTATATTTAGGCTACGAACAATGGTTGCCTAGTCCACCAAAGATACAGAAGCCTAGTTCCCCAAAGATACAGAAACTCAGTCCACCAAAGGTGCAGAAGCCTCAGACTGTACTGAATGCAGCGGCATTAGCTTACCTCGGTGACTGCATATATGAGCTTTATGCTCGCCGACATTTTTTGTTCCCCCCCTTGAGTATTGAAGAATATAATGATCGTGTGATGGCCGTTGTACGTTGTGAAGCACAGGATGCATTGCTCCAGAAACTTATGAAGGACAACTTTCTAACAGAAGAAGAGAGGAATGTTCTTCGGTGGGGAAAGAACATAAGTTCAGCCAAGACACGAACGAAAAAGCGTGCTGGTGCAGCAGTCTATAACAGAGCATCTTCACTGGAGACATTAATTGGACATCTCTATCTGACAGATATGAAACGTTTGGAAGAAGTAATGGTGAAGTTGGGATTTTTGACTGACTCTTCTCAACTGATTCCATCAGAGGATGTGAATGAGCCAACATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

250

Amino Acids

28.12

Weight (kDa)

9.43

Isoelectric Point (pI)

54.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 118 - 218 9.7e-21 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 125
AciI CCGC 3 cut(s) 82, 159, 350
AclI AACGTT 1 cut(s) 661
AcoI YGGCCR 1 cut(s) 450
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 4 cut(s) 77, 175, 339, 459
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 1 cut(s) 428
AluBI AGCT 5 cut(s) 47, 111, 196, 359, 385
AluI AGCT 5 cut(s) 47, 111, 196, 359, 385
Alw26I GTCTC 1 cut(s) 617
AoxI GGCC 1 cut(s) 450
ApeKI GCWGC 2 cut(s) 347, 593
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AseI ATTAAT 1 cut(s) 629
AsuHPI GGTGA 3 cut(s) 6, 380, 691
BbvI GCAGC 2 cut(s) 359, 605
BccI CCATC 2 cut(s) 442, 727
BceAI ACGGC 1 cut(s) 437
BciVI GTATCC 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 617
BfaI CTAG 3 cut(s) 258, 282, 748
BfuI GTATCC 1 cut(s) 49
BisI GCNGC 3 cut(s) 348, 351, 594
BlsI GCNGC 3 cut(s) 349, 352, 595
BmsI GCATC 2 cut(s) 466, 620
BpmI CTGGAG 3 cut(s) 97, 470, 641
BpuEI CTTGAG 1 cut(s) 439
BsaJI CCNNGG 1 cut(s) 364
BsaXI ACNNNNNCTCC 4 cut(s) 36, 54, 66, 84
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 624
Bse3DI GCAATG 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 364
BseGI GGATG 4 cut(s) 229, 481, 733, 743
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMI GCAATG 1 cut(s) 479
BseMII CTCAG 2 cut(s) 319, 344
BseNI ACTGG 1 cut(s) 624
BseXI GCAGC 2 cut(s) 359, 605
BsgI GTGCAG 2 cut(s) 341, 612
BshFI GGCC 1 cut(s) 452
BsiWI CGTACG 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 1 cut(s) 617
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 1 cut(s) 452
Bsp143I GATC 2 cut(s) 99, 439
BspACI CCGC 3 cut(s) 82, 159, 350
BspANI GGCC 1 cut(s) 452
BspCNI CTCAG 2 cut(s) 318, 343
BsrDI GCAATG 1 cut(s) 479
BsrI ACTGG 1 cut(s) 624
BssECI CCNNGG 1 cut(s) 364
BssMI GATC 2 cut(s) 99, 439
Bst4CI ACNGT 3 cut(s) 148, 178, 337
Bst6I CTCTTC 2 cut(s) 516, 709
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 3 cut(s) 6, 394, 585
BstDEI CTNAG 2 cut(s) 305, 330
BstF5I GGATG 4 cut(s) 229, 481, 733, 743
BstKTI GATC 2 cut(s) 102, 442
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 2 cut(s) 99, 439
BstMWI GCNNNNNNNGC 3 cut(s) 356, 476, 593
BstNSI RCATGY 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 359, 605
BsuI GTATCC 1 cut(s) 49
BsuRI GGCC 1 cut(s) 452
BtsCI GGATG 4 cut(s) 229, 481, 733, 743
BtsIMutI CAGTG 2 cut(s) 57, 617
Cac8I GCNNGC 3 cut(s) 6, 394, 585
Csp6I GTAC 4 cut(s) 76, 174, 338, 458
CviAII CATG 2 cut(s) 5, 185
CviQI GTAC 4 cut(s) 76, 174, 338, 458
DdeI CTNAG 2 cut(s) 305, 330
DpnI GATC 2 cut(s) 101, 441
DpnII GATC 2 cut(s) 99, 439
EaeI YGGCCR 1 cut(s) 450
Eam1104I CTCTTC 2 cut(s) 516, 709
EarI CTCTTC 2 cut(s) 516, 709
Eco57I CTGAAG 1 cut(s) 33
EcoT22I ATGCAT 2 cut(s) 6, 481
FaeI CATG 2 cut(s) 8, 188
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FatI CATG 2 cut(s) 4, 184
FblI GTMKAC 1 cut(s) 125
Fnu4HI GCNGC 3 cut(s) 348, 351, 594
FokI GGATG 4 cut(s) 236, 488, 730, 740
Fsp4HI GCNGC 3 cut(s) 348, 351, 594
FspBI CTAG 3 cut(s) 258, 282, 748
GluI GCNGC 3 cut(s) 348, 351, 594
GsuI CTGGAG 3 cut(s) 97, 470, 641
HaeIII GGCC 1 cut(s) 452
Hin1II CATG 2 cut(s) 8, 188
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 3 cut(s) 69, 701, 715
HphI GGTGA 3 cut(s) 6, 380, 691
Hpy166II GTNNAC 4 cut(s) 126, 181, 263, 311
Hpy188I TCNGA 4 cut(s) 156, 333, 648, 724
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 4 cut(s) 126, 181, 263, 311
HpyAV CCTTC 1 cut(s) 493
HpyCH4III ACNGT 3 cut(s) 148, 178, 337
HpyCH4IV ACGT 2 cut(s) 460, 661
HpyCH4V TGCA 6 cut(s) 4, 322, 347, 375, 479, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 476, 593
HpyF3I CTNAG 2 cut(s) 305, 330
HpySE526I ACGT 2 cut(s) 460, 661
Hsp92II CATG 2 cut(s) 8, 188
Kzo9I GATC 2 cut(s) 99, 439
LmnI GCTCC 3 cut(s) 44, 116, 489
LpnPI CCDG 5 cut(s) 127, 458, 500, 573, 605
Lsp1109I GCAGC 2 cut(s) 359, 605
LweI GCATC 2 cut(s) 466, 620
MaeI CTAG 3 cut(s) 258, 282, 748
MaeII ACGT 2 cut(s) 460, 661
MaeIII GTNAC 2 cut(s) 148, 368
MalI GATC 2 cut(s) 101, 441
MboI GATC 2 cut(s) 99, 439
MboII GAAGA 7 cut(s) 440, 527, 530, 533, 606, 680, 696
MluCI AATT 1 cut(s) 630
MlyI GAGTC 2 cut(s) 63, 695
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 7 cut(s) 21, 150, 172, 339, 374, 519, 718
Mph1103I ATGCAT 2 cut(s) 6, 481
MseI TTAA 1 cut(s) 629
MspA1I CMGCKG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 356, 476, 593
NdeII GATC 2 cut(s) 99, 439
NlaIII CATG 2 cut(s) 8, 188
NmuCI GTSAC 2 cut(s) 148, 368
NsiI ATGCAT 2 cut(s) 6, 481
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PctI GAATGC 1 cut(s) 349
PfeI GAWTC 1 cut(s) 715
Pfl23II CGTACG 1 cut(s) 173
PkrI GCNGC 3 cut(s) 349, 352, 595
PleI GAGTC 2 cut(s) 63, 695
PpsI GAGTC 2 cut(s) 63, 695
PshBI ATTAAT 1 cut(s) 629
Psp1406I AACGTT 1 cut(s) 661
PspLI CGTACG 1 cut(s) 173
RsaI GTAC 4 cut(s) 77, 175, 339, 459
RsaNI GTAC 4 cut(s) 76, 174, 338, 458
SaqAI TTAA 1 cut(s) 629
SatI GCNGC 3 cut(s) 348, 351, 594
Sau3AI GATC 2 cut(s) 99, 439
SchI GAGTC 2 cut(s) 63, 695
SetI ASST 9 cut(s) 49, 113, 198, 321, 361, 366, 387, 463, 664
SfaNI GCATC 2 cut(s) 466, 620
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
SphI GCATGC 1 cut(s) 8
Sse9I AATT 1 cut(s) 630
SsiI CCGC 3 cut(s) 82, 159, 350
SspMI CTAG 3 cut(s) 258, 282, 748
TaaI ACNGT 3 cut(s) 148, 178, 337
TaiI ACGT 2 cut(s) 463, 664
TasI AATT 1 cut(s) 630
TatI WGTACW 1 cut(s) 337
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 1 cut(s) 715
Tru1I TTAA 1 cut(s) 629
Tru9I TTAA 1 cut(s) 629
TscAI CASTG 2 cut(s) 57, 624
TseFI GTSAC 2 cut(s) 148, 368
TseI GCWGC 2 cut(s) 347, 593
Tsp45I GTSAC 2 cut(s) 148, 368
TspDTI ATGAA 2 cut(s) 512, 671
TspRI CASTG 2 cut(s) 57, 624
VspI ATTAAT 1 cut(s) 629
XceI RCATGY 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 125
XspI CTAG 3 cut(s) 258, 282, 748
Zsp2I ATGCAT 2 cut(s) 6, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.