Rorug06G0028500

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003592
Physical Location & Seq
Reverse (-)
3396317 .. 3397686
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0028500.1

Sequence Viewer

Length: 924 bp
ATGTGTTTTTACGAAGTTGACATTAAACTTGAGCAACATGTTCGAGAACTGAAAGAAGAGGTGAATAGAATGCTAATTGACTCCTTCAAAAACCCTTCACAACAATTGGACTTGGTTGATGACATTCAACGCCTAGGCGTGTCCTATCATTTTGAAAATGAGATTGATGAAATTTTGAAACAAATTCACCACAACTACTCTTATGGTAGTGAAGATGACCTTTACACTACTGCTCTCCGTTTTCGATTGCTGAGACAACAAGGTTACAATGTTTCATGCGATAATATGATATATAAAGGCTCGTATAATGGATTCCCTGGTAATGCAGATATGTTCAACAAGTACATGGAAGCAAATGATCAAAAATTTAAGGCATCTCCCTTGAGTGATCCGCCCATCTTAGGATACACAGAGAAGACATACTTGAAGAGGCTAGCTCTAGCTTTCAGCACCACTCATCTTGAGTCGATCAAACACGGTTTAAGCCCTCCACTTTCGAAACAAGTAGCTCATTCCTTAACTCAGCCACTTCGAAAGGGCATCACCAGGGTGGAAGCTAAGTATTACTTGTCAATCTATCATGAACGCGATTCACATAACGAGACTCTCCTAACTTTTGCAAAGTTGGATTTCAACCTACTGCAGCAAGTCCATCAAAAGGAACTATGTGAAATTACAAGGTGGTGGAAGGACTTGGACGTGAGAAACACGTTGCCGTTTACAAGAGACAGAGTTACCGAAGTTTACTTCAGCTGGGCTTTGTCAGTGTCCTTCGAACCTCAATATTCCTTTGCTAGGAGGACATCATGCAAAGTCACCGCCATGACATCTATTATCGATGACATTTATGATGCCCATGGCACACTCGAAGAATTAGAGCTCTTTACTAAAGCTATTCAGAGGTTAATTTATTATTTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

307

Amino Acids

36.1

Weight (kDa)

6.01

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 9 - 173 1e-32 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 230 - 301 1.2e-24 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 271 - 302 1.5e-06 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 588
AciI CCGC 2 cut(s) 392, 819
AclWI GGATC 1 cut(s) 383
AcsI RAATTY 3 cut(s) 171, 183, 365
AcuI CTGAAG 1 cut(s) 733
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 3 cut(s) 401, 658, 795
AflIII ACRYGT 2 cut(s) 37, 708
AgsI TTSAA 7 cut(s) 88, 128, 155, 178, 337, 427, 634
AjiI CACGTC 1 cut(s) 700
AjnI CCWGG 2 cut(s) 316, 545
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 7 cut(s) 437, 443, 509, 557, 753, 880, 893
AluI AGCT 7 cut(s) 437, 443, 509, 557, 753, 880, 893
Alw21I GWGCWC 1 cut(s) 882
Alw26I GTCTC 3 cut(s) 247, 596, 720
AlwI GGATC 1 cut(s) 383
ApeKI GCWGC 1 cut(s) 643
ApoI RAATTY 3 cut(s) 171, 183, 365
AspA2I CCTAGG 1 cut(s) 133
AsuHPI GGTGA 4 cut(s) 73, 179, 535, 808
AsuII TTCGAA 3 cut(s) 497, 532, 774
AsuNHI GCTAGC 1 cut(s) 433
AvrII CCTAGG 1 cut(s) 133
BanII GRGCYC 1 cut(s) 882
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 882
BbvI GCAGC 1 cut(s) 655
BccI CCATC 2 cut(s) 404, 660
BceAI ACGGC 1 cut(s) 700
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BciT130I CCWGG 2 cut(s) 318, 547
BciVI GTATCC 1 cut(s) 398
BclI TGATCA 1 cut(s) 358
BcoDI GTCTC 3 cut(s) 247, 596, 720
BfaI CTAG 4 cut(s) 134, 434, 440, 795
BfmI CTRYAG 1 cut(s) 641
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 1 cut(s) 644
BlnI CCTAGG 1 cut(s) 133
BlsI GCNGC 1 cut(s) 645
Bme1390I CCNGG 2 cut(s) 318, 547
BmgBI CACGTC 1 cut(s) 700
BmrFI CCNGG 2 cut(s) 318, 547
BmsI GCATC 3 cut(s) 383, 549, 841
BmtI GCTAGC 1 cut(s) 437
BpiI GAAGAC 1 cut(s) 422
BplI GAGNNNNNCTC 2 cut(s) 421, 453
Bpu14I TTCGAA 3 cut(s) 497, 532, 774
BpuEI CTTGAG 3 cut(s) 50, 403, 482
Bsa29I ATCGAT 1 cut(s) 837
BsaJI CCNNGG 4 cut(s) 133, 316, 546, 856
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 658, 795
BseBI CCWGG 2 cut(s) 318, 547
BseCI ATCGAT 1 cut(s) 837
BseDI CCNNGG 4 cut(s) 133, 316, 546, 856
BseLI CCNNNNNNNGG 3 cut(s) 401, 658, 795
BseMII CTCAG 2 cut(s) 242, 536
BseXI GCAGC 1 cut(s) 655
BseYI CCCAGC 1 cut(s) 753
Bsh1236I CGCG 1 cut(s) 588
BshVI ATCGAT 1 cut(s) 837
BsiHKAI GWGCWC 1 cut(s) 882
BslI CCNNNNNNNGG 3 cut(s) 401, 658, 795
BsmAI GTCTC 3 cut(s) 247, 596, 720
BsmI GAATGC 1 cut(s) 75
Bsp119I TTCGAA 3 cut(s) 497, 532, 774
Bsp1286I GDGCHC 1 cut(s) 882
Bsp143I GATC 3 cut(s) 358, 388, 468
Bsp19I CCATGG 1 cut(s) 856
BspACI CCGC 2 cut(s) 392, 819
BspCNI CTCAG 2 cut(s) 243, 535
BspDI ATCGAT 1 cut(s) 837
BspFNI CGCG 1 cut(s) 588
BspHI TCATGA 1 cut(s) 580
BspMAI CTGCAG 1 cut(s) 645
BspOI GCTAGC 1 cut(s) 437
BspPI GGATC 1 cut(s) 383
BspT104I TTCGAA 3 cut(s) 497, 532, 774
BssECI CCNNGG 4 cut(s) 133, 316, 546, 856
BssMI GATC 3 cut(s) 358, 388, 468
BssT1I CCWWGG 2 cut(s) 133, 856
Bst2UI CCWGG 2 cut(s) 318, 547
Bst4CI ACNGT 1 cut(s) 479
Bst6I CTCTTC 2 cut(s) 51, 422
BstBI TTCGAA 3 cut(s) 497, 532, 774
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 4 cut(s) 251, 400, 522, 558
BstDSI CCRYGG 1 cut(s) 856
BstENI CCTNNNNNAGG 1 cut(s) 793
BstFNI CGCG 1 cut(s) 588
BstKTI GATC 3 cut(s) 361, 391, 471
BstMAI GTCTC 3 cut(s) 247, 596, 720
BstMBI GATC 3 cut(s) 358, 388, 468
BstNI CCWGG 2 cut(s) 318, 547
BstNSI RCATGY 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 316, 545
BstSFI CTRYAG 1 cut(s) 641
BstUI CGCG 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 655
BstV2I GAAGAC 1 cut(s) 422
Bsu15I ATCGAT 1 cut(s) 837
BsuI GTATCC 1 cut(s) 398
BsuTUI ATCGAT 1 cut(s) 837
BtgI CCRYGG 1 cut(s) 856
BtrI CACGTC 1 cut(s) 700
BtsIMutI CAGTG 1 cut(s) 771
Cac8I GCNNGC 1 cut(s) 435
CciI TCATGA 1 cut(s) 580
ClaI ATCGAT 1 cut(s) 837
Csp6I GTAC 1 cut(s) 343
CspCI CAANNNNNGTGG 2 cut(s) 442, 477
CviAII CATG 7 cut(s) 38, 276, 346, 581, 807, 823, 857
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 4 cut(s) 251, 400, 522, 558
DpnI GATC 3 cut(s) 360, 390, 470
DpnII GATC 3 cut(s) 358, 388, 468
DraI TTTAAA 1 cut(s) 918
Eam1104I CTCTTC 2 cut(s) 51, 422
EarI CTCTTC 2 cut(s) 51, 422
EciI GGCGGA 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 2 cut(s) 133, 856
Eco24I GRGCYC 1 cut(s) 882
Eco53kI GAGCTC 1 cut(s) 880
Eco57I CTGAAG 1 cut(s) 733
EcoICRI GAGCTC 1 cut(s) 880
EcoNI CCTNNNNNAGG 1 cut(s) 793
EcoRII CCWGG 2 cut(s) 316, 545
EcoT14I CCWWGG 2 cut(s) 133, 856
EcoT38I GRGCYC 1 cut(s) 882
ErhI CCWWGG 2 cut(s) 133, 856
FaeI CATG 7 cut(s) 41, 279, 349, 584, 810, 826, 860
FalI AAGNNNNNCTT 6 cut(s) 204, 236, 407, 439, 551, 583
FatI CATG 7 cut(s) 37, 275, 345, 580, 806, 822, 856
FbaI TGATCA 1 cut(s) 358
Fnu4HI GCNGC 1 cut(s) 644
FriOI GRGCYC 1 cut(s) 882
Fsp4HI GCNGC 1 cut(s) 644
FspBI CTAG 4 cut(s) 134, 434, 440, 795
GluI GCNGC 1 cut(s) 644
GsaI CCCAGC 1 cut(s) 757
Hin1II CATG 7 cut(s) 41, 279, 349, 584, 810, 826, 860
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HinfI GANTC 5 cut(s) 80, 312, 464, 590, 604
HphI GGTGA 4 cut(s) 73, 179, 535, 808
Hpy166II GTNNAC 3 cut(s) 19, 720, 745
Hpy188I TCNGA 1 cut(s) 900
Hpy188III TCNNGA 3 cut(s) 44, 461, 581
Hpy8I GTNNAC 3 cut(s) 19, 720, 745
HpyAV CCTTC 4 cut(s) 94, 105, 682, 781
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4IV ACGT 2 cut(s) 699, 710
HpyCH4V TGCA 4 cut(s) 326, 620, 643, 810
HpyF3I CTNAG 4 cut(s) 251, 400, 522, 558
HpySE526I ACGT 2 cut(s) 699, 710
Hsp92II CATG 7 cut(s) 41, 279, 349, 584, 810, 826, 860
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 3 cut(s) 358, 388, 468
LpnPI CCDG 5 cut(s) 303, 330, 532, 559, 739
Lsp1109I GCAGC 1 cut(s) 655
LweI GCATC 3 cut(s) 383, 549, 841
MaeI CTAG 4 cut(s) 134, 434, 440, 795
MaeII ACGT 2 cut(s) 699, 710
MaeIII GTNAC 3 cut(s) 263, 733, 814
MalI GATC 3 cut(s) 360, 390, 470
MboI GATC 3 cut(s) 358, 388, 468
MboII GAAGA 5 cut(s) 68, 224, 427, 439, 881
MfeI CAATTG 1 cut(s) 104
MhlI GDGCHC 1 cut(s) 882
MluCI AATT 8 cut(s) 75, 104, 171, 183, 365, 672, 872, 906
MlyI GAGTC 3 cut(s) 74, 473, 598
MmeI TCCRAC 1 cut(s) 606
MnlI CCTC 6 cut(s) 52, 423, 498, 789, 792, 894
MseI TTAA 6 cut(s) 24, 369, 482, 518, 905, 917
MslI CAYNNNNRTG 2 cut(s) 548, 821
MspA1I CMGCKG 1 cut(s) 753
MspR9I CCNGG 2 cut(s) 318, 547
MunI CAATTG 1 cut(s) 104
Mva1269I GAATGC 1 cut(s) 75
MvaI CCWGG 2 cut(s) 318, 547
MvnI CGCG 1 cut(s) 588
NcoI CCATGG 1 cut(s) 856
NdeII GATC 3 cut(s) 358, 388, 468
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 7 cut(s) 41, 279, 349, 584, 810, 826, 860
NmuCI GTSAC 1 cut(s) 814
NspI RCATGY 1 cut(s) 41
NspV TTCGAA 3 cut(s) 497, 532, 774
OliI CACNNNNGTG 1 cut(s) 548
PagI TCATGA 1 cut(s) 580
PciI ACATGT 1 cut(s) 37
PctI GAATGC 1 cut(s) 75
PfeI GAWTC 2 cut(s) 312, 590
PkrI GCNGC 1 cut(s) 645
PleI GAGTC 3 cut(s) 74, 472, 598
PpsI GAGTC 3 cut(s) 74, 472, 598
PscI ACATGT 1 cut(s) 37
Psp124BI GAGCTC 1 cut(s) 882
Psp6I CCWGG 2 cut(s) 316, 545
PspFI CCCAGC 1 cut(s) 753
PspGI CCWGG 2 cut(s) 316, 545
PstI CTGCAG 1 cut(s) 645
PvuII CAGCTG 1 cut(s) 753
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 2 cut(s) 548, 821
SacI GAGCTC 1 cut(s) 882
SaqAI TTAA 6 cut(s) 24, 369, 482, 518, 905, 917
SatI GCNGC 1 cut(s) 644
Sau3AI GATC 3 cut(s) 358, 388, 468
SchI GAGTC 3 cut(s) 74, 473, 598
ScrFI CCNGG 2 cut(s) 318, 547
SduI GDGCHC 1 cut(s) 882
SfaNI GCATC 3 cut(s) 383, 549, 841
SfcI CTRYAG 1 cut(s) 641
SfuI TTCGAA 3 cut(s) 497, 532, 774
SmiMI CAYNNNNRTG 2 cut(s) 548, 821
SmlI CTYRAG 3 cut(s) 29, 382, 461
SmoI CTYRAG 3 cut(s) 29, 382, 461
Sse9I AATT 8 cut(s) 75, 104, 171, 183, 365, 672, 872, 906
SsiI CCGC 2 cut(s) 392, 819
SspI AATATT 1 cut(s) 785
SspMI CTAG 4 cut(s) 134, 434, 440, 795
SstI GAGCTC 1 cut(s) 882
StyD4I CCNGG 2 cut(s) 316, 545
StyI CCWWGG 2 cut(s) 133, 856
TaaI ACNGT 1 cut(s) 479
TaiI ACGT 2 cut(s) 702, 713
TaqI TCGA 8 cut(s) 43, 244, 467, 497, 532, 774, 837, 867
TasI AATT 8 cut(s) 75, 104, 171, 183, 365, 672, 872, 906
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 2 cut(s) 312, 590
Tru1I TTAA 6 cut(s) 24, 369, 482, 518, 905, 917
Tru9I TTAA 6 cut(s) 24, 369, 482, 518, 905, 917
TscAI CASTG 1 cut(s) 771
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 1 cut(s) 643
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 3 cut(s) 183, 264, 597
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 771
XagI CCTNNNNNAGG 1 cut(s) 793
XapI RAATTY 3 cut(s) 171, 183, 365
XceI RCATGY 1 cut(s) 41
XmaJI CCTAGG 1 cut(s) 133
XspI CTAG 4 cut(s) 134, 434, 440, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.