Rroxscaffold_7G00201220

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003592
Physical Location & Seq
Reverse (-)
48791966 .. 48796634
4669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00201220.1

Sequence Viewer

Length: 801 bp
ATGCTGTTCATTTTCTCAATGATGCATTCTGGAAAGTCTTCATCGTGGGAATCTTATCTTGTAGAGGAGAGAGTAGGAATGGATGAGTTATATATGGGCTACGACCGATGGTTGCCTAGTCCACCGCAGGTACAGAAGCCTAGTTCTTCCAAGGTACAGAAGCCTAGTACACCAAAGGTACAGAAGCCTCAGACCGTACTCAATGCAGTCTCATTAGCTTACCTCGGCGACTGTATTTATGAGATAGAACTGCTTCCTGTTATAATTGCGACATATACAGTTATGCAATGTGTTTATATGGACTTAACTGCTGTTTTTAAAAGCAGCTATATGCTCGCAGGCACTTTTTGTTCCCCCTTGAGTATTGAAGAGTATAATGATCGTGTGATGGCTGTTGTACGCTGTGAAGCACAGGATGCACTGCTCCAGAGACTTCTAAAGGACAATTTTTTATCAGAAGAAGAGAGGAATGTCCTTAGGTGGGGAAAGAACATCAGTTCAGCTAAAACACGAACAAAGAAGCGTGCTGGTGCAGCAGTCTATAACAGAGCATCTTCACTGGAAACATTAGTTGGACATCTCTACCTCACGGATGTAAAGCGTTTGGAAGAAGTCATGGTGAAGTTGGGGTTCTCAACTGATTCTTCTATGCAGTTGACATCAGAAGATGCAAATGGTAAGTCTGCACCAGCATCAGAAGATGCAAATGGTAAGTTTGCACCAGCTTCTTCTATGTACAGTGTACATGTATCTGTGCTTAGTTTGCTGAAGAACTTCCCCTTCAAAGATGGCTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

266

Amino Acids

29.67

Weight (kDa)

6.84

Isoelectric Point (pI)

55.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 72 - 197 2.4e-11 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 118
AciI CCGC 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 788
AfaI GTAC 8 cut(s) 132, 156, 169, 180, 198, 399, 737, 744
AfiI CCNNNNNNNGG 1 cut(s) 481
AflIII ACRYGT 1 cut(s) 745
AgsI TTSAA 2 cut(s) 368, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 555, 587
AluBI AGCT 4 cut(s) 218, 327, 503, 725
AluI AGCT 4 cut(s) 218, 327, 503, 725
Alw26I GTCTC 2 cut(s) 214, 424
ApeKI GCWGC 2 cut(s) 324, 533
ArsI GACNNNNNNTTYG 2 cut(s) 665, 697
Asp700I GAANNNNTTC 3 cut(s) 37, 252, 773
AsuHPI GGTGA 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 336, 545
BccI CCATC 3 cut(s) 102, 382, 782
BcoDI GTCTC 2 cut(s) 214, 424
BfaI CTAG 3 cut(s) 117, 141, 165
BfuAI ACCTGC 1 cut(s) 118
BisI GCNGC 2 cut(s) 325, 534
BlsI GCNGC 2 cut(s) 326, 535
BmsI GCATC 6 cut(s) 12, 406, 560, 658, 691, 701
BpiI GAAGAC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 150, 223
Bsc4I CCNNNNNNNGG 1 cut(s) 481
Bse1I ACTGG 1 cut(s) 564
Bse21I CCTNAGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 293
BseDI CCNNGG 2 cut(s) 150, 223
BseGI GGATG 3 cut(s) 88, 421, 598
BseLI CCNNNNNNNGG 1 cut(s) 481
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 564
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 2 cut(s) 336, 545
BsgI GTGCAG 2 cut(s) 552, 669
Bsh1285I CGRYCG 1 cut(s) 106
BsiEI CGRYCG 1 cut(s) 106
BslI CCNNNNNNNGG 1 cut(s) 481
BsmAI GTCTC 2 cut(s) 214, 424
BsmI GAATGC 1 cut(s) 25
Bsp1407I TGTACA 2 cut(s) 735, 742
Bsp143I GATC 1 cut(s) 379
BspACI CCGC 1 cut(s) 125
BspCNI CTCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 293
BsrGI TGTACA 2 cut(s) 735, 742
BsrI ACTGG 1 cut(s) 564
BssECI CCNNGG 2 cut(s) 150, 223
BssMI GATC 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 4 cut(s) 196, 233, 280, 740
Bst6I CTCTTC 2 cut(s) 363, 456
BstAPI GCANNNNNTGC 1 cut(s) 416
BstAUI TGTACA 2 cut(s) 735, 742
BstC8I GCNNGC 3 cut(s) 336, 340, 525
BstDEI CTNAG 3 cut(s) 189, 476, 758
BstF5I GGATG 3 cut(s) 88, 421, 598
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 2 cut(s) 214, 424
BstMBI GATC 1 cut(s) 379
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 3 cut(s) 416, 533, 763
BstNSI RCATGY 1 cut(s) 749
BstV1I GCAGC 2 cut(s) 336, 545
BstV2I GAAGAC 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 476
BtsCI GGATG 3 cut(s) 88, 421, 598
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 3 cut(s) 419, 557, 745
BveI ACCTGC 1 cut(s) 118
Cac8I GCNNGC 3 cut(s) 336, 340, 525
Csp6I GTAC 8 cut(s) 131, 155, 168, 179, 197, 398, 736, 743
CviAII CATG 2 cut(s) 616, 746
CviQI GTAC 8 cut(s) 131, 155, 168, 179, 197, 398, 736, 743
DdeI CTNAG 3 cut(s) 189, 476, 758
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
DraI TTTAAA 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 363, 456
EarI CTCTTC 2 cut(s) 363, 456
Eco130I CCWWGG 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 788
Eco81I CCTNAGG 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 619, 749
FatI CATG 2 cut(s) 615, 745
Fnu4HI GCNGC 2 cut(s) 325, 534
FokI GGATG 3 cut(s) 95, 428, 605
Fsp4HI GCNGC 2 cut(s) 325, 534
FspBI CTAG 3 cut(s) 117, 141, 165
GluI GCNGC 2 cut(s) 325, 534
GsuI CTGGAG 1 cut(s) 410
Hin1II CATG 2 cut(s) 619, 749
HincII GTYRAC 1 cut(s) 657
HindII GTYRAC 1 cut(s) 657
HinfI GANTC 2 cut(s) 50, 641
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 4 cut(s) 122, 170, 657, 743
Hpy188I TCNGA 4 cut(s) 192, 457, 664, 697
Hpy188III TCNNGA 2 cut(s) 30, 427
Hpy8I GTNNAC 4 cut(s) 122, 170, 657, 743
HpyAV CCTTC 1 cut(s) 790
HpyCH4III ACNGT 4 cut(s) 196, 233, 280, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 416, 533, 763
HpyF3I CTNAG 3 cut(s) 189, 476, 758
Hsp92II CATG 2 cut(s) 619, 749
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 1 cut(s) 429
Lsp1109I GCAGC 2 cut(s) 336, 545
LweI GCATC 6 cut(s) 12, 406, 560, 658, 691, 701
MaeI CTAG 3 cut(s) 117, 141, 165
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MluCI AATT 2 cut(s) 264, 445
MmeI TCCRAC 1 cut(s) 553
MnlI CCTC 5 cut(s) 58, 198, 233, 459, 596
Mph1103I ATGCAT 1 cut(s) 27
MroXI GAANNNNTTC 3 cut(s) 37, 252, 773
MseI TTAA 2 cut(s) 305, 318
Mva1269I GAATGC 1 cut(s) 25
MwoI GCNNNNNNNGC 3 cut(s) 416, 533, 763
NdeII GATC 1 cut(s) 379
NlaIII CATG 2 cut(s) 619, 749
NmeAIII GCCGAG 1 cut(s) 204
NsiI ATGCAT 1 cut(s) 27
NspI RCATGY 1 cut(s) 749
PciI ACATGT 1 cut(s) 745
PctI GAATGC 1 cut(s) 25
PdmI GAANNNNTTC 3 cut(s) 37, 252, 773
PfeI GAWTC 2 cut(s) 50, 641
PkrI GCNGC 2 cut(s) 326, 535
PscI ACATGT 1 cut(s) 745
PsiI TTATAA 1 cut(s) 263
RsaI GTAC 8 cut(s) 132, 156, 169, 180, 198, 399, 737, 744
RsaNI GTAC 8 cut(s) 131, 155, 168, 179, 197, 398, 736, 743
SaqAI TTAA 2 cut(s) 305, 318
SatI GCNGC 2 cut(s) 325, 534
Sau3AI GATC 1 cut(s) 379
SfaNI GCATC 6 cut(s) 12, 406, 560, 658, 691, 701
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
Sse9I AATT 2 cut(s) 264, 445
SsiI CCGC 1 cut(s) 125
SspMI CTAG 3 cut(s) 117, 141, 165
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 4 cut(s) 196, 233, 280, 740
TaqII GACCGA 1 cut(s) 120
TasI AATT 2 cut(s) 264, 445
TatI WGTACW 3 cut(s) 167, 735, 742
TfiI GAWTC 2 cut(s) 50, 641
Tru1I TTAA 2 cut(s) 305, 318
Tru9I TTAA 2 cut(s) 305, 318
TscAI CASTG 3 cut(s) 426, 564, 745
TseI GCWGC 2 cut(s) 324, 533
TspDTI ATGAA 1 cut(s) 30
TspGWI ACGGA 1 cut(s) 605
TspRI CASTG 3 cut(s) 426, 564, 745
XceI RCATGY 1 cut(s) 749
XmnI GAANNNNTTC 3 cut(s) 37, 252, 773
XspI CTAG 3 cut(s) 117, 141, 165
Zsp2I ATGCAT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.