AT3G13740

Ribonuclease III family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003592
Physical Location & Seq
Forward (+)
4504067 .. 4506320
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G13740.3

Sequence Viewer

Length: 744 bp
ATGGTGATTTTATCGGCGAGCTCCACTGTGAGAGCTGCCTTAGATACACAACCTAGACTTCCCTACAACCCTAATGCGCCGCGAAAAGTTAAGAAAATCCCTAACGGTAATTCATTCTTACCGCCGCCGTCACCTGCACCGTCTCCAGGAATCAGCATCTCCGTCGCCGATTTGCTCAAGCGTCCTTCAAGTAAAGAGATAACTGTTGACGTTGATGATACTTATATGGGATATGAGACATGGTCTCCAAGTCCACCAAAGTTGGAGAAACCAAGATCAGTCTTCAATCCTGCTTCTTTAGCCTTTATAGGCGATTCCATTTATGAGCTATATGCTCGTAGGCATTTTCTTTTCCCTCCGCTTAGTATTGAAGATTATAACGATCGTGTTAGAGCTGTGGTGCGCTGTGAAGCTCAGTATGCTTTGCTGAATAAACTTGTTGATGATGACTTTCTAACAAAGGAAGAGAGGGATATTCTTAGGTGGGGAAAGAATCTAGGTTCTGCTAGAACACGTACAAGAAGGCGTGCAGGTAATGCGGTTTATAACAAGGCATCATCATTGGAAACACTAATTGGTTATCTGTATCTGACAAACGGGAAAAGATTAGAAAAAATAATGGAGAAGCTAGGATTCTCAAGTGATTCTTCAACTGAGATCGTGATTGAAGAAACCAACCCTAAACCATCAGAATCATCCAACCTGCCAAACTTCATACTCAACGAGCAAGTGGTGTCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

247

Amino Acids

27.68

Weight (kDa)

9.04

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 99 - 200 8.7e-22 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 378, 546
AarI CACCTGC 1 cut(s) 142
Acc36I ACCTGC 3 cut(s) 142, 521, 711
AccII CGCG 1 cut(s) 82
AciI CCGC 5 cut(s) 80, 122, 125, 359, 539
AfaI GTAC 1 cut(s) 517
AfiI CCNNNNNNNGG 1 cut(s) 146
AflIII ACRYGT 1 cut(s) 512
AgsI TTSAA 5 cut(s) 189, 286, 371, 651, 668
AjnI CCWGG 1 cut(s) 145
AjuI GAANNNNNNNTTGG 2 cut(s) 558, 590
AluBI AGCT 6 cut(s) 21, 35, 328, 395, 413, 628
AluI AGCT 6 cut(s) 21, 35, 328, 395, 413, 628
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 3 cut(s) 147, 230, 249
ApeKI GCWGC 1 cut(s) 35
AspLEI GCGC 2 cut(s) 79, 405
AsuHPI GGTGA 2 cut(s) 16, 123
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 22
BccI CCATC 1 cut(s) 694
BceAI ACGGC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BciT130I CCWGG 1 cut(s) 147
BcoDI GTCTC 3 cut(s) 147, 230, 249
BfaI CTAG 4 cut(s) 54, 497, 507, 629
BfuAI ACCTGC 3 cut(s) 142, 521, 711
BisI GCNGC 3 cut(s) 36, 80, 125
BlsI GCNGC 3 cut(s) 37, 81, 126
Bme1390I CCNGG 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 2 cut(s) 165, 563
BpiI GAAGAC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 129
BpuEI CTTGAG 2 cut(s) 161, 622
BsaAI YACGTR 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 1 cut(s) 146
BseBI CCWGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 695
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 2 cut(s) 428, 645
BseXI GCAGC 1 cut(s) 22
BsgI GTGCAG 2 cut(s) 120, 549
Bsh1236I CGCG 1 cut(s) 82
Bsh1285I CGRYCG 1 cut(s) 385
BsiEI CGRYCG 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 147, 230, 249
BsmBI CGTCTC 1 cut(s) 147
Bso31I GGTCTC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 3 cut(s) 275, 382, 657
BspACI CCGC 5 cut(s) 80, 122, 125, 359, 539
BspCNI CTCAG 2 cut(s) 427, 646
BspFNI CGCG 1 cut(s) 82
BspMI ACCTGC 3 cut(s) 142, 521, 711
BspTNI GGTCTC 1 cut(s) 249
BssMI GATC 3 cut(s) 275, 382, 657
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 4 cut(s) 28, 107, 141, 205
Bst6I CTCTTC 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBAI YACGTR 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 19, 528
BstDEI CTNAG 5 cut(s) 40, 362, 414, 479, 654
BstF5I GGATG 1 cut(s) 695
BstFNI CGCG 1 cut(s) 82
BstHHI GCGC 2 cut(s) 79, 405
BstKTI GATC 3 cut(s) 278, 385, 660
BstMAI GTCTC 3 cut(s) 147, 230, 249
BstMBI GATC 3 cut(s) 275, 382, 657
BstMCI CGRYCG 1 cut(s) 385
BstMWI GCNNNNNNNGC 3 cut(s) 299, 419, 536
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BstUI CGCG 1 cut(s) 82
BstV1I GCAGC 1 cut(s) 22
BstV2I GAAGAC 1 cut(s) 274
BtsCI GGATG 1 cut(s) 695
BtsIMutI CAGTG 1 cut(s) 24
BveI ACCTGC 3 cut(s) 142, 521, 711
Cac8I GCNNGC 2 cut(s) 19, 528
CfoI GCGC 2 cut(s) 79, 405
CseI GACGC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 516
CspCI CAANNNNNGTGG 2 cut(s) 243, 278
CviAII CATG 1 cut(s) 240
CviJI RGCY 7 cut(s) 21, 35, 302, 328, 395, 413, 628
CviKI_1 RGCY 7 cut(s) 21, 35, 302, 328, 395, 413, 628
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 5 cut(s) 40, 362, 414, 479, 654
DpnI GATC 3 cut(s) 277, 384, 659
DpnII GATC 3 cut(s) 275, 382, 657
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 1 cut(s) 243
FalI AAGNNNNNCTT 2 cut(s) 631, 663
FatI CATG 1 cut(s) 239
Fnu4HI GCNGC 3 cut(s) 36, 80, 125
FokI GGATG 1 cut(s) 682
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 3 cut(s) 36, 80, 125
FspBI CTAG 4 cut(s) 54, 497, 507, 629
GlaI GCGC 2 cut(s) 78, 404
GluI GCNGC 3 cut(s) 36, 80, 125
GsuI CTGGAG 1 cut(s) 129
HgaI GACGC 1 cut(s) 170
HhaI GCGC 2 cut(s) 79, 405
Hin1II CATG 1 cut(s) 243
Hin6I GCGC 2 cut(s) 77, 403
HinP1I GCGC 2 cut(s) 77, 403
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 6 cut(s) 150, 314, 493, 633, 644, 692
HphI GGTGA 2 cut(s) 16, 123
Hpy166II GTNNAC 2 cut(s) 208, 254
Hpy188I TCNGA 2 cut(s) 591, 691
Hpy188III TCNNGA 1 cut(s) 661
Hpy8I GTNNAC 2 cut(s) 208, 254
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 2 cut(s) 195, 516
HpyCH4III ACNGT 4 cut(s) 28, 107, 141, 205
HpyCH4IV ACGT 2 cut(s) 210, 514
HpyCH4V TGCA 2 cut(s) 137, 530
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 419, 536
HpyF3I CTNAG 5 cut(s) 40, 362, 414, 479, 654
HpySE526I ACGT 2 cut(s) 210, 514
Hsp92II CATG 1 cut(s) 243
HspAI GCGC 2 cut(s) 77, 403
Kzo9I GATC 3 cut(s) 275, 382, 657
LmnI GCTCC 1 cut(s) 26
LpnPI CCDG 6 cut(s) 132, 147, 159, 303, 516, 716
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 2 cut(s) 165, 563
MaeI CTAG 4 cut(s) 54, 497, 507, 629
MaeII ACGT 2 cut(s) 210, 514
MaeIII GTNAC 1 cut(s) 129
MalI GATC 3 cut(s) 277, 384, 659
MboI GATC 3 cut(s) 275, 382, 657
MboII GAAGA 5 cut(s) 274, 383, 476, 639, 680
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 2 cut(s) 109, 573
MmeI TCCRAC 2 cut(s) 243, 723
MnlI CCTC 2 cut(s) 366, 462
MseI TTAA 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 3 cut(s) 299, 419, 536
NdeII GATC 3 cut(s) 275, 382, 657
NlaIII CATG 1 cut(s) 243
NmuCI GTSAC 1 cut(s) 129
PaqCI CACCTGC 1 cut(s) 142
PfeI GAWTC 6 cut(s) 150, 314, 493, 633, 644, 692
PflFI GACNNNGTC 1 cut(s) 241
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 3 cut(s) 37, 81, 126
Ple19I CGATCG 1 cut(s) 385
Ppu21I YACGTR 1 cut(s) 515
PsiI TTATAA 2 cut(s) 378, 546
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PsyI GACNNNGTC 1 cut(s) 241
PvuI CGATCG 1 cut(s) 385
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 3 cut(s) 36, 80, 125
Sau3AI GATC 3 cut(s) 275, 382, 657
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 23
SfaNI GCATC 2 cut(s) 165, 563
SmlI CTYRAG 2 cut(s) 176, 637
SmoI CTYRAG 2 cut(s) 176, 637
Sse9I AATT 2 cut(s) 109, 573
SsiI CCGC 5 cut(s) 80, 122, 125, 359, 539
SspMI CTAG 4 cut(s) 54, 497, 507, 629
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 4 cut(s) 28, 107, 141, 205
TaiI ACGT 2 cut(s) 213, 517
TasI AATT 2 cut(s) 109, 573
TauI GCSGC 2 cut(s) 82, 127
TfiI GAWTC 6 cut(s) 150, 314, 493, 633, 644, 692
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 129
TspDTI ATGAA 2 cut(s) 102, 703
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 31
Tth111I GACNNNGTC 1 cut(s) 241
XspI CTAG 4 cut(s) 54, 497, 507, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.