Rh4DG170200

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003592
Physical Location & Seq
Reverse (-)
33934509 .. 33936602
2094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG170200.1

Sequence Viewer

Length: 300 bp
ATGAGGCACTTTTTGTTTCCCCCCTTGAGTATTGAAGAATGCAATGACCGTGTGAAGGCAGTTGTACGTTGTGAAGCACAGGATGCATTGCTTCAGAAACTAATGAAGGACAAATGTTTAACAGAAGAAGAGAGGAATGTTCTTCAGTGGGGAAAGAACATAAGTTCAGCTAAGACACGAACCAAAAAGCATGCTGGTGCAGCAGTCTATAACAGAGCATCTTCACTGGAGACATTAGTTGGACATTTCTATCTGACGGATATGAAACGTTTGGAAGAAGTAATGGTGAGGTTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

99

Amino Acids

11.43

Weight (kDa)

9.12

Isoelectric Point (pI)

56.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 4 - 87 5e-12 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 268
AcuI CTGAAG 2 cut(s) 77, 128
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 1 cut(s) 35
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw26I GTCTC 1 cut(s) 224
ApeKI GCWGC 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 298
BbvI GCAGC 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 224
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
BmsI GCATC 2 cut(s) 73, 227
BpmI CTGGAG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 2 cut(s) 49, 86
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 2 cut(s) 49, 86
BseNI ACTGG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 212
BsgI GTGCAG 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 49, 86
BsrI ACTGG 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 50
Bst6I CTCTTC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 224
BstMWI GCNNNNNNNGC 2 cut(s) 83, 200
BstNSI RCATGY 1 cut(s) 194
BstV1I GCAGC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 2 cut(s) 152, 224
Cac8I GCNNGC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 65
CviAII CATG 1 cut(s) 191
CviJI RGCY 1 cut(s) 170
CviKI_1 RGCY 1 cut(s) 170
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco57I CTGAAG 2 cut(s) 77, 128
EcoT22I ATGCAT 1 cut(s) 88
FaeI CATG 1 cut(s) 194
FaiI YATR 4 cut(s) 161, 192, 210, 263
FatI CATG 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 248
Hin1II CATG 1 cut(s) 194
HphI GGTGA 1 cut(s) 298
Hpy188I TCNGA 2 cut(s) 96, 255
HpyAV CCTTC 2 cut(s) 49, 100
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4IV ACGT 2 cut(s) 67, 268
HpyCH4V TGCA 3 cut(s) 42, 86, 200
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 200
HpyF3I CTNAG 1 cut(s) 171
HpySE526I ACGT 2 cut(s) 67, 268
Hsp92II CATG 1 cut(s) 194
LpnPI CCDG 3 cut(s) 65, 180, 212
Lsp1109I GCAGC 1 cut(s) 212
LweI GCATC 2 cut(s) 73, 227
MaeII ACGT 2 cut(s) 67, 268
MboII GAAGA 6 cut(s) 47, 134, 137, 140, 213, 287
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 2 cut(s) 126, 282
Mph1103I ATGCAT 1 cut(s) 88
MseI TTAA 1 cut(s) 119
MslI CAYNNNNRTG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 44
MwoI GCNNNNNNNGC 2 cut(s) 83, 200
NlaIII CATG 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 88
NspI RCATGY 1 cut(s) 194
PaeI GCATGC 1 cut(s) 194
PctI GAATGC 1 cut(s) 44
PkrI GCNGC 1 cut(s) 202
Psp1406I AACGTT 1 cut(s) 268
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 195
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 1 cut(s) 201
SetI ASST 4 cut(s) 70, 172, 271, 293
SfaNI GCATC 2 cut(s) 73, 227
SgeI CNNG 7 cut(s) 37, 62, 92, 189, 203, 207, 239
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
SphI GCATGC 1 cut(s) 194
TaaI ACNGT 1 cut(s) 50
TaiI ACGT 2 cut(s) 70, 271
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 2 cut(s) 152, 231
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 119, 278
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 2 cut(s) 152, 231
XceI RCATGY 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.