RLG00000014049

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003592
Physical Location & Seq
Reverse (-)
46197385 .. 46201913
4529 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014049

Sequence Viewer

Length: 777 bp
ATGCATGCTATATCAGTGCCTTCACCGATGAGTGTGAGAGTAGTGAGAGTGGGAGCTTCTTGGGATACCCAACAAAGACTCCCATACAACCGCAACGCCCCAAGAAAGTTAAAGCAAACCCCCTCCTCCACCACTATTCCCACCACCGAAACCCCTCCAACTGTCTCTCTAACCGCCTCAACTACCCGTACGGCCGACCACGTCTTTGAACTTCTCAAACGCCCAACTCAAGAGGAGAGAGTAGGAATGGATGAGTTATATATGGGCTACGACCGATGGTTGCCTAGTCCACCCAAGGTACAGAAGCCTAGTTCTCCCAAGGTACAGAAGCCTAGTACACCAAAGGTACAGAAGCCTCAGACCGTACTCAATGCAGTCTCATTAGCTTACCTCGGCGACTGTATTTATGAGCTATATGCTCGCAGGCACTTTTTGTTTCCCCCCTTGAGTATTGAAGAGTATAATGATCGTGTGATGGCTGTTGTACGCTGTGAAGCACAGGACGCACTGCTCCTGAGACTTCTAAAGGACAATTTTTTATCAGAAGAAGAGAGGAATGTCCTTAGGTGGGGAAAGAACATCAGTTCAGCTAAAACACGAACAAAGAAGCGTGCTGGTGCAGCGGTCTATAACAGAGCATCTTCACTGGAAACATTAGTTGGACATCTCTACCTGACAGATATGAAGCGTTTGGAAGAAGTCATGGTGAAGTTGGGGTTCTCAACTGATTCTTCTATGCAGTTGACATCAGAGGATGCAAATGGGCAAGCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

259

Amino Acids

29.08

Weight (kDa)

9.54

Isoelectric Point (pI)

57.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 127 - 227 4.5e-19 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 91, 174, 623
AcoI YGGCCR 1 cut(s) 192
AfaI GTAC 7 cut(s) 190, 300, 324, 337, 348, 366, 486
AfiI CCNNNNNNNGG 1 cut(s) 568
AgsI TTSAA 2 cut(s) 209, 455
AjiI CACGTC 1 cut(s) 202
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AluBI AGCT 4 cut(s) 56, 386, 412, 590
AluI AGCT 4 cut(s) 56, 386, 412, 590
Alw26I GTCTC 3 cut(s) 169, 382, 511
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 620
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AsuHPI GGTGA 2 cut(s) 15, 718
AxyI CCTNAGG 1 cut(s) 563
BbvI GCAGC 1 cut(s) 632
BccI CCATC 2 cut(s) 270, 469
BceAI ACGGC 1 cut(s) 207
BciVI GTATCC 1 cut(s) 58
BcoDI GTCTC 3 cut(s) 169, 382, 511
BfaI CTAG 4 cut(s) 285, 309, 333, 775
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 1 cut(s) 621
BlsI GCNGC 1 cut(s) 622
BmgBI CACGTC 1 cut(s) 202
BmsI GCATC 2 cut(s) 647, 745
BpuEI CTTGAG 2 cut(s) 213, 466
BsaJI CCNNGG 3 cut(s) 294, 318, 391
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 568
Bse1I ACTGG 1 cut(s) 651
Bse21I CCTNAGG 1 cut(s) 563
BseDI CCNNGG 3 cut(s) 294, 318, 391
BseGI GGATG 3 cut(s) 256, 760, 770
BseLI CCNNNNNNNGG 1 cut(s) 568
BseMII CTCAG 2 cut(s) 371, 506
BseNI ACTGG 1 cut(s) 651
BseRI GAGGAG 2 cut(s) 115, 248
BseX3I CGGCCG 1 cut(s) 192
BseXI GCAGC 1 cut(s) 632
BsgI GTGCAG 1 cut(s) 639
Bsh1285I CGRYCG 2 cut(s) 195, 274
BshFI GGCC 1 cut(s) 194
BsiEI CGRYCG 2 cut(s) 195, 274
BsiWI CGTACG 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 568
BsmAI GTCTC 3 cut(s) 169, 382, 511
BsnI GGCC 1 cut(s) 194
Bsp143I GATC 1 cut(s) 466
BspACI CCGC 3 cut(s) 91, 174, 623
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 2 cut(s) 370, 507
BsrI ACTGG 1 cut(s) 651
BssECI CCNNGG 3 cut(s) 294, 318, 391
BssMI GATC 1 cut(s) 466
BssT1I CCWWGG 2 cut(s) 294, 318
Bst4CI ACNGT 3 cut(s) 163, 364, 401
Bst6I CTCTTC 2 cut(s) 450, 543
BstC8I GCNNGC 5 cut(s) 6, 421, 425, 612, 768
BstDEI CTNAG 3 cut(s) 357, 515, 563
BstF5I GGATG 3 cut(s) 256, 760, 770
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 3 cut(s) 169, 382, 511
BstMBI GATC 1 cut(s) 466
BstMCI CGRYCG 2 cut(s) 195, 274
BstMWI GCNNNNNNNGC 2 cut(s) 503, 620
BstNSI RCATGY 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 632
BstZI CGGCCG 1 cut(s) 192
Bsu36I CCTNAGG 1 cut(s) 563
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 1 cut(s) 194
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 3 cut(s) 256, 760, 770
BtsI GCAGTG 1 cut(s) 506
BtsIMutI CAGTG 3 cut(s) 21, 506, 644
Cac8I GCNNGC 5 cut(s) 6, 421, 425, 612, 768
CseI GACGC 1 cut(s) 512
Csp6I GTAC 7 cut(s) 189, 299, 323, 336, 347, 365, 485
CviAII CATG 2 cut(s) 5, 703
CviQI GTAC 7 cut(s) 189, 299, 323, 336, 347, 365, 485
DdeI CTNAG 3 cut(s) 357, 515, 563
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 192
EagI CGGCCG 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 450, 543
EarI CTCTTC 2 cut(s) 450, 543
EclXI CGGCCG 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 294, 318
Eco52I CGGCCG 1 cut(s) 192
Eco81I CCTNAGG 1 cut(s) 563
EcoT14I CCWWGG 2 cut(s) 294, 318
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 294, 318
FaeI CATG 2 cut(s) 8, 706
FatI CATG 2 cut(s) 4, 702
Fnu4HI GCNGC 1 cut(s) 621
FokI GGATG 3 cut(s) 263, 757, 767
Fsp4HI GCNGC 1 cut(s) 621
FspBI CTAG 4 cut(s) 285, 309, 333, 775
GluI GCNGC 1 cut(s) 621
HaeIII GGCC 1 cut(s) 194
HgaI GACGC 1 cut(s) 512
Hin1II CATG 2 cut(s) 8, 706
HincII GTYRAC 1 cut(s) 744
HindII GTYRAC 1 cut(s) 744
HinfI GANTC 2 cut(s) 78, 728
HphI GGTGA 2 cut(s) 15, 718
Hpy166II GTNNAC 3 cut(s) 290, 338, 744
Hpy188I TCNGA 3 cut(s) 360, 544, 751
Hpy188III TCNNGA 2 cut(s) 230, 514
Hpy8I GTNNAC 3 cut(s) 290, 338, 744
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 3 cut(s) 163, 364, 401
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 5 cut(s) 4, 374, 620, 739, 758
HpyF10VI GCNNNNNNNGC 2 cut(s) 503, 620
HpyF3I CTNAG 3 cut(s) 357, 515, 563
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 2 cut(s) 8, 706
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 53, 516
LpnPI CCDG 6 cut(s) 409, 485, 527, 600, 632, 686
Lsp1109I GCAGC 1 cut(s) 632
LweI GCATC 2 cut(s) 647, 745
MaeI CTAG 4 cut(s) 285, 309, 333, 775
MaeII ACGT 1 cut(s) 201
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 6 cut(s) 467, 557, 560, 633, 707, 723
MluCI AATT 1 cut(s) 532
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 2 cut(s) 182, 640
MnlI CCTC 9 cut(s) 133, 136, 165, 187, 226, 366, 401, 546, 745
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 623
MwoI GCNNNNNNNGC 2 cut(s) 503, 620
NdeII GATC 1 cut(s) 466
NlaIII CATG 2 cut(s) 8, 706
NmeAIII GCCGAG 1 cut(s) 372
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 728
Pfl23II CGTACG 1 cut(s) 188
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 622
PleI GAGTC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 72
PspLI CGTACG 1 cut(s) 188
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 7 cut(s) 190, 300, 324, 337, 348, 366, 486
RsaNI GTAC 7 cut(s) 189, 299, 323, 336, 347, 365, 485
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 1 cut(s) 621
Sau3AI GATC 1 cut(s) 466
SchI GAGTC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 647, 745
SmlI CTYRAG 2 cut(s) 228, 445
SmoI CTYRAG 2 cut(s) 228, 445
SphI GCATGC 1 cut(s) 8
Sse9I AATT 1 cut(s) 532
SsiI CCGC 3 cut(s) 91, 174, 623
SspMI CTAG 4 cut(s) 285, 309, 333, 775
StyI CCWWGG 2 cut(s) 294, 318
TaaI ACNGT 3 cut(s) 163, 364, 401
TaiI ACGT 1 cut(s) 204
TaqII GACCGA 1 cut(s) 288
TasI AATT 1 cut(s) 532
TatI WGTACW 1 cut(s) 335
TfiI GAWTC 1 cut(s) 728
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 3 cut(s) 21, 513, 651
TseI GCWGC 1 cut(s) 620
TspDTI ATGAA 1 cut(s) 698
TspRI CASTG 3 cut(s) 21, 513, 651
Tth111I GACNNNGTC 1 cut(s) 200
XceI RCATGY 1 cut(s) 8
XspI CTAG 4 cut(s) 285, 309, 333, 775
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.