MD09G1194100.v1.1

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003592
Physical Location & Seq
Reverse (-)
17435273 .. 17438785
3513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1194100.v1.1.491

Sequence Viewer

Length: 768 bp
ATGCAAACTCTATCAGTGGCTTTTCCGCCGATGAGTGCGAGAGTAGTGACAGTGAGAGCTTCCGTGGATACACAGCAAAGACTCTCATACAACCGCAACGCCCCAAGAAAGATACAGAAAAGCGAAAGCCCCGCCACCGCCAAACCCCCTCCAACCGTCACACTAACCACCACAACTACCCGAACGGCCGACTCCGTTGTTGACCTCCTCAAACGCACACCTCAAGGGGAAACAGTACAAAAGGATGAGTTATACTTGGGCTATGAGCGATGGTCGCCTACTCCAACAAAGGTACAGAAGCGTTTGCCTAGTCCACCAAAGGTACAGAAGCCTCGGTCTGTACTCAATGCGGCATCGCTAGCTTATATCGGTGATTGCATTTACGAGCTATATGCTCGCAGGCATTTTTTGTTTCCTCCCCTGAATATTGAAGAATATAATGATCGTGTGATGGCAGTTGTGCGTTGTGAAGCACAGGATGCACTGCTCCGGAAACTTCTGAGTGATGATTTTTTATCAGAAGAAGAAAGGAATGTTCTCCGGTGGGGAAAGAATATCGGTTCAGCTAAAACACGAACAAAAAAGCGCGCTGGTTCAGCAGCTTATAACAGAGCATCTTCACTGGAAGCATTGCTTGGTTATCTCTACCTGACAAATATGGAACGTTTAGAAGAAGTCATGATAAAGTTGGGATTCTCAACTGATTCTTCCGTGCAGATAATTTCAGAGGAGCCTGAGGAGGTAAACGGCACGCATCCTAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

256

Amino Acids

28.71

Weight (kDa)

9.24

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 119 - 219 4.9e-22 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AccII CGCG 1 cut(s) 588
AccIII TCCGGA 1 cut(s) 489
AciI CCGC 5 cut(s) 26, 94, 132, 138, 350
AclI AACGTT 1 cut(s) 664
AcoI YGGCCR 1 cut(s) 186
AfaI GTAC 4 cut(s) 237, 294, 324, 342
AgsI TTSAA 1 cut(s) 431
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AluBI AGCT 5 cut(s) 59, 362, 388, 566, 602
AluI AGCT 5 cut(s) 59, 362, 388, 566, 602
Aor13HI TCCGGA 1 cut(s) 489
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 599
AspLEI GCGC 2 cut(s) 588, 590
AsuHPI GGTGA 1 cut(s) 383
AsuNHI GCTAGC 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 735
BbvI GCAGC 1 cut(s) 611
BccI CCATC 2 cut(s) 264, 445
BceAI ACGGC 2 cut(s) 201, 763
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciVI GTATCC 1 cut(s) 61
BfaI CTAG 3 cut(s) 309, 359, 759
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 2 cut(s) 351, 600
BlsI GCNGC 2 cut(s) 352, 601
BmiI GGNNCC 1 cut(s) 732
BmsI GCATC 4 cut(s) 362, 469, 623, 763
BmtI GCTAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 63, 332
BsaWI WCCGGW 2 cut(s) 489, 540
Bse1I ACTGG 1 cut(s) 627
Bse21I CCTNAGG 1 cut(s) 735
Bse3DI GCAATG 1 cut(s) 629
BseAI TCCGGA 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 63, 332
BseGI GGATG 3 cut(s) 250, 484, 754
BseMI GCAATG 1 cut(s) 629
BseMII CTCAG 2 cut(s) 491, 726
BseNI ACTGG 1 cut(s) 627
BsePI GCGCGC 1 cut(s) 586
BseRI GAGGAG 3 cut(s) 197, 743, 752
BseX3I CGGCCG 1 cut(s) 186
BseXI GCAGC 1 cut(s) 611
BsgI GTGCAG 1 cut(s) 734
Bsh1236I CGCG 1 cut(s) 588
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 189
BsiSI CCGG 2 cut(s) 490, 541
BsnI GGCC 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 489
Bsp143I GATC 1 cut(s) 442
BspACI CCGC 5 cut(s) 26, 94, 132, 138, 350
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 2 cut(s) 492, 727
BspEI TCCGGA 1 cut(s) 489
BspFNI CGCG 1 cut(s) 588
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 1 cut(s) 732
BspOI GCTAGC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 629
BsrI ACTGG 1 cut(s) 627
BssECI CCNNGG 2 cut(s) 63, 332
BssHII GCGCGC 1 cut(s) 586
BssMI GATC 1 cut(s) 442
Bst4CI ACNGT 3 cut(s) 52, 157, 235
BstAPI GCANNNNNTGC 1 cut(s) 479
BstC8I GCNNGC 5 cut(s) 360, 397, 401, 588, 752
BstDEI CTNAG 2 cut(s) 500, 735
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 3 cut(s) 250, 484, 754
BstFNI CGCG 1 cut(s) 588
BstHHI GCGC 2 cut(s) 588, 590
BstKTI GATC 1 cut(s) 445
BstMBI GATC 1 cut(s) 442
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 4 cut(s) 274, 359, 479, 596
BstUI CGCG 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 611
BstZI CGGCCG 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 735
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 2 cut(s) 283, 339
BtsCI GGATG 3 cut(s) 250, 484, 754
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 4 cut(s) 21, 57, 482, 620
Cac8I GCNNGC 5 cut(s) 360, 397, 401, 588, 752
CciI TCATGA 1 cut(s) 678
CfoI GCGC 2 cut(s) 588, 590
Csp6I GTAC 4 cut(s) 236, 293, 323, 341
CviAII CATG 1 cut(s) 679
CviQI GTAC 4 cut(s) 236, 293, 323, 341
DdeI CTNAG 2 cut(s) 500, 735
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
EaeI YGGCCR 1 cut(s) 186
EagI CGGCCG 1 cut(s) 186
EciI GGCGGA 1 cut(s) 15
EclXI CGGCCG 1 cut(s) 186
Eco52I CGGCCG 1 cut(s) 186
Eco81I CCTNAGG 1 cut(s) 735
FaeI CATG 1 cut(s) 682
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FatI CATG 1 cut(s) 678
FauI CCCGC 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 351, 600
FokI GGATG 3 cut(s) 257, 491, 741
Fsp4HI GCNGC 2 cut(s) 351, 600
FspBI CTAG 3 cut(s) 309, 359, 759
GlaI GCGC 2 cut(s) 587, 589
GluI GCNGC 2 cut(s) 351, 600
HaeIII GGCC 1 cut(s) 188
HapII CCGG 2 cut(s) 490, 541
HhaI GCGC 2 cut(s) 588, 590
Hin1II CATG 1 cut(s) 682
Hin6I GCGC 2 cut(s) 586, 588
HinP1I GCGC 2 cut(s) 586, 588
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 4 cut(s) 81, 191, 693, 704
HpaII CCGG 2 cut(s) 490, 541
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 3 cut(s) 202, 314, 745
Hpy188I TCNGA 3 cut(s) 501, 520, 727
Hpy188III TCNNGA 2 cut(s) 490, 679
Hpy8I GTNNAC 3 cut(s) 202, 314, 745
HpyCH4III ACNGT 3 cut(s) 52, 157, 235
HpyCH4IV ACGT 1 cut(s) 664
HpyCH4V TGCA 4 cut(s) 4, 378, 482, 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 359, 479, 596
HpyF3I CTNAG 2 cut(s) 500, 735
HpySE526I ACGT 1 cut(s) 664
Hsp92II CATG 1 cut(s) 682
HspAI GCGC 2 cut(s) 586, 588
Kpn2I TCCGGA 1 cut(s) 489
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 2 cut(s) 492, 730
LpnPI CCDG 9 cut(s) 385, 434, 461, 503, 554, 576, 608, 662, 747
Lsp1109I GCAGC 1 cut(s) 611
LweI GCATC 4 cut(s) 362, 469, 623, 763
MaeI CTAG 3 cut(s) 309, 359, 759
MaeII ACGT 1 cut(s) 664
MaeIII GTNAC 2 cut(s) 46, 157
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 6 cut(s) 443, 533, 536, 609, 683, 699
MluCI AATT 1 cut(s) 720
MlyI GAGTC 2 cut(s) 75, 185
MmeI TCCRAC 2 cut(s) 176, 308
MnlI CCTC 9 cut(s) 159, 215, 218, 231, 342, 426, 721, 730, 733
MroI TCCGGA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 759
MspI CCGG 2 cut(s) 490, 541
MvnI CGCG 1 cut(s) 588
MwoI GCNNNNNNNGC 4 cut(s) 274, 359, 479, 596
NdeII GATC 1 cut(s) 442
NheI GCTAGC 1 cut(s) 358
NlaIII CATG 1 cut(s) 682
NlaIV GGNNCC 1 cut(s) 732
NmuCI GTSAC 2 cut(s) 46, 157
PagI TCATGA 1 cut(s) 678
PauI GCGCGC 1 cut(s) 586
PfeI GAWTC 2 cut(s) 693, 704
PkrI GCNGC 2 cut(s) 352, 601
PleI GAGTC 2 cut(s) 75, 185
PpsI GAGTC 2 cut(s) 75, 185
PsiI TTATAA 1 cut(s) 606
Psp1406I AACGTT 1 cut(s) 664
PspN4I GGNNCC 1 cut(s) 732
PteI GCGCGC 1 cut(s) 586
RsaI GTAC 4 cut(s) 237, 294, 324, 342
RsaNI GTAC 4 cut(s) 236, 293, 323, 341
RseI CAYNNNNRTG 1 cut(s) 759
SatI GCNGC 2 cut(s) 351, 600
Sau3AI GATC 1 cut(s) 442
SchI GAGTC 2 cut(s) 75, 185
SfaNI GCATC 4 cut(s) 362, 469, 623, 763
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
Sse9I AATT 1 cut(s) 720
SsiI CCGC 5 cut(s) 26, 94, 132, 138, 350
SspI AATATT 1 cut(s) 427
SspMI CTAG 3 cut(s) 309, 359, 759
TaaI ACNGT 3 cut(s) 52, 157, 235
TaiI ACGT 1 cut(s) 667
TaqII GACCGA 1 cut(s) 324
TasI AATT 1 cut(s) 720
TatI WGTACW 2 cut(s) 235, 340
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 2 cut(s) 693, 704
TscAI CASTG 4 cut(s) 21, 57, 489, 627
TseFI GTSAC 2 cut(s) 46, 157
TseI GCWGC 1 cut(s) 599
Tsp45I GTSAC 2 cut(s) 46, 157
TspGWI ACGGA 3 cut(s) 52, 184, 700
TspRI CASTG 4 cut(s) 21, 57, 489, 627
XspI CTAG 3 cut(s) 309, 359, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.