Rh6BG151000

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003592
Physical Location & Seq
Forward (+)
24258530 .. 24263788
5259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG151000.1

Sequence Viewer

Length: 726 bp
ATGCATGCTATATCAGTGCCTTCACCGATGAGTGTGAGACTAGTGAGAGTGGGAGCTTCTTGGGATACCCAACAAAGACTCCCATACAACCGCAACGCCCCAAGAAAGTTAAAGCAAACCCCCTCCTCCACCACTATTCCCACCCCCGAAACCCCTCCAACTGTCTCTCTAACTGCCTCAACTACCCGTACGGCCGACCACGTCTTTGAACTTCTCAAACGCCCAACTCAAGAGGAGAGAGTCGGAATGGATGAGTTATATATGGGCTACGACCGATGGTTGCCTAGTCCACCTAAGGTACAGAAGCCTAGTTCTCCCAAGGTACAGAAGCCTAGTACACCAAAGGTACAGAAGCCTCAGACCGTACTCAATGCAGTCTCATTAGCTTACCTCGGCGACTGTATTTATGAGCTATATGCTCGCAGGCACTTTTTGTTCCCCCCCTTGAGTATTGAAGAGTATAATGATCGTGTGATGGCTGTTGTACGCTGTGAAGCACAGGATGCACTGCTCCAGAGACTTCTAAAGGACAATTTTTTATCAGAAGAAGAGAGGAATGTCCTTAGGTGGGGAAAGAACATCAGTTCAGCTAAAACACGAACAAAGAAGCGTGCTGGTGCAGCAGTCTATAACAGAGCATCTTCACTGGAAACATTAGTCAGTGCCCCCTTGTGCTTTCCTTGGACTTTTTTCATCAACTCTAGGCTTTGGAATATTGTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

241

Amino Acids

27.48

Weight (kDa)

9.86

Isoelectric Point (pI)

61.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 127 - 222 8.6e-17 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 91
AcoI YGGCCR 1 cut(s) 192
AfaI GTAC 7 cut(s) 190, 300, 324, 337, 348, 366, 486
AfiI CCNNNNNNNGG 1 cut(s) 568
AgsI TTSAA 2 cut(s) 209, 455
AhlI ACTAGT 1 cut(s) 40
AjiI CACGTC 1 cut(s) 202
AluBI AGCT 4 cut(s) 56, 386, 412, 590
AluI AGCT 4 cut(s) 56, 386, 412, 590
Alw26I GTCTC 4 cut(s) 31, 169, 382, 511
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 620
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AsuHPI GGTGA 1 cut(s) 15
AxyI CCTNAGG 2 cut(s) 294, 563
BaeGI GKGCMC 1 cut(s) 667
BbvI GCAGC 1 cut(s) 632
BccI CCATC 2 cut(s) 270, 469
BceAI ACGGC 1 cut(s) 207
BciVI GTATCC 1 cut(s) 58
BcoDI GTCTC 4 cut(s) 31, 169, 382, 511
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 5 cut(s) 41, 285, 309, 333, 702
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 1 cut(s) 621
BlsI GCNGC 1 cut(s) 622
BmgBI CACGTC 1 cut(s) 202
BmsI GCATC 2 cut(s) 493, 647
BpmI CTGGAG 1 cut(s) 497
BpuEI CTTGAG 2 cut(s) 213, 466
BsaJI CCNNGG 3 cut(s) 318, 391, 680
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 568
Bse1I ACTGG 1 cut(s) 651
Bse21I CCTNAGG 2 cut(s) 294, 563
BseDI CCNNGG 3 cut(s) 318, 391, 680
BseGI GGATG 2 cut(s) 256, 508
BseLI CCNNNNNNNGG 1 cut(s) 568
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 651
BseRI GAGGAG 2 cut(s) 115, 248
BseSI GKGCMC 1 cut(s) 667
BseX3I CGGCCG 1 cut(s) 192
BseXI GCAGC 1 cut(s) 632
BsgI GTGCAG 1 cut(s) 639
Bsh1285I CGRYCG 2 cut(s) 195, 274
BshFI GGCC 1 cut(s) 194
BsiEI CGRYCG 2 cut(s) 195, 274
BsiWI CGTACG 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 568
BsmAI GTCTC 4 cut(s) 31, 169, 382, 511
BsnI GGCC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 667
Bsp143I GATC 1 cut(s) 466
BspACI CCGC 1 cut(s) 91
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 1 cut(s) 370
BsrI ACTGG 1 cut(s) 651
BssECI CCNNGG 3 cut(s) 318, 391, 680
BssMI GATC 1 cut(s) 466
BssT1I CCWWGG 2 cut(s) 318, 680
Bst4CI ACNGT 3 cut(s) 163, 364, 401
Bst6I CTCTTC 2 cut(s) 450, 543
BstAPI GCANNNNNTGC 1 cut(s) 503
BstC8I GCNNGC 4 cut(s) 6, 421, 425, 612
BstDEI CTNAG 3 cut(s) 294, 357, 563
BstF5I GGATG 2 cut(s) 256, 508
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 4 cut(s) 31, 169, 382, 511
BstMBI GATC 1 cut(s) 466
BstMCI CGRYCG 2 cut(s) 195, 274
BstMWI GCNNNNNNNGC 2 cut(s) 503, 620
BstNSI RCATGY 1 cut(s) 8
BstSLI GKGCMC 1 cut(s) 667
BstV1I GCAGC 1 cut(s) 632
BstZI CGGCCG 1 cut(s) 192
Bsu36I CCTNAGG 2 cut(s) 294, 563
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 1 cut(s) 194
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 2 cut(s) 256, 508
BtsI GCAGTG 1 cut(s) 506
BtsIMutI CAGTG 4 cut(s) 21, 506, 644, 667
Cac8I GCNNGC 4 cut(s) 6, 421, 425, 612
Csp6I GTAC 7 cut(s) 189, 299, 323, 336, 347, 365, 485
CviAII CATG 1 cut(s) 5
CviQI GTAC 7 cut(s) 189, 299, 323, 336, 347, 365, 485
DdeI CTNAG 3 cut(s) 294, 357, 563
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 192
EagI CGGCCG 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 450, 543
EarI CTCTTC 2 cut(s) 450, 543
EclXI CGGCCG 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 318, 680
Eco52I CGGCCG 1 cut(s) 192
Eco81I CCTNAGG 2 cut(s) 294, 563
EcoT14I CCWWGG 2 cut(s) 318, 680
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 318, 680
FaeI CATG 1 cut(s) 8
FatI CATG 1 cut(s) 4
Fnu4HI GCNGC 1 cut(s) 621
FokI GGATG 2 cut(s) 263, 515
Fsp4HI GCNGC 1 cut(s) 621
FspBI CTAG 5 cut(s) 41, 285, 309, 333, 702
GluI GCNGC 1 cut(s) 621
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 1 cut(s) 194
Hin1II CATG 1 cut(s) 8
HinfI GANTC 2 cut(s) 78, 240
HphI GGTGA 1 cut(s) 15
Hpy166II GTNNAC 2 cut(s) 290, 338
Hpy188I TCNGA 3 cut(s) 245, 360, 544
Hpy188III TCNNGA 2 cut(s) 230, 514
Hpy8I GTNNAC 2 cut(s) 290, 338
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 3 cut(s) 163, 364, 401
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 4 cut(s) 4, 374, 506, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 503, 620
HpyF3I CTNAG 3 cut(s) 294, 357, 563
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 1 cut(s) 8
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 53, 516
LpnPI CCDG 5 cut(s) 409, 485, 527, 600, 632
Lsp1109I GCAGC 1 cut(s) 632
LweI GCATC 2 cut(s) 493, 647
MaeI CTAG 5 cut(s) 41, 285, 309, 333, 702
MaeII ACGT 1 cut(s) 201
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 4 cut(s) 467, 557, 560, 633
MhlI GDGCHC 1 cut(s) 667
MluCI AATT 1 cut(s) 532
MlyI GAGTC 2 cut(s) 72, 249
MmeI TCCRAC 2 cut(s) 182, 223
MnlI CCTC 8 cut(s) 133, 136, 165, 187, 226, 366, 401, 546
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 503, 620
NdeII GATC 1 cut(s) 466
NlaIII CATG 1 cut(s) 8
NmeAIII GCCGAG 1 cut(s) 372
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
Pfl23II CGTACG 1 cut(s) 188
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 622
PleI GAGTC 2 cut(s) 72, 248
PpsI GAGTC 2 cut(s) 72, 248
PspLI CGTACG 1 cut(s) 188
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 7 cut(s) 190, 300, 324, 337, 348, 366, 486
RsaNI GTAC 7 cut(s) 189, 299, 323, 336, 347, 365, 485
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 1 cut(s) 621
Sau3AI GATC 1 cut(s) 466
SchI GAGTC 2 cut(s) 72, 249
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 2 cut(s) 493, 647
SmlI CTYRAG 2 cut(s) 228, 445
SmoI CTYRAG 2 cut(s) 228, 445
SpeI ACTAGT 1 cut(s) 40
SphI GCATGC 1 cut(s) 8
Sse9I AATT 1 cut(s) 532
SsiI CCGC 1 cut(s) 91
SspI AATATT 1 cut(s) 715
SspMI CTAG 5 cut(s) 41, 285, 309, 333, 702
StyI CCWWGG 2 cut(s) 318, 680
TaaI ACNGT 3 cut(s) 163, 364, 401
TaiI ACGT 1 cut(s) 204
TaqII GACCGA 1 cut(s) 288
TasI AATT 1 cut(s) 532
TatI WGTACW 1 cut(s) 335
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 4 cut(s) 21, 513, 651, 667
TseI GCWGC 1 cut(s) 620
TspDTI ATGAA 1 cut(s) 682
TspRI CASTG 4 cut(s) 21, 513, 651, 667
Tth111I GACNNNGTC 1 cut(s) 200
XceI RCATGY 1 cut(s) 8
XspI CTAG 5 cut(s) 41, 285, 309, 333, 702
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.