Rw4G014630

Ribonuclease III domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003592
Physical Location & Seq
Reverse (-)
33811516 .. 33815787
4272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014630.1

Sequence Viewer

Length: 729 bp
ATGCATGCTGTATCAGCGATGAGTTTGAGAGAAGTGAGAGTGGAAGCTTCATTGGATACGCAACAAAGACTCCCATACAACCGGAACGCTCCAAGAAAGATAAAGGACAGCTCCAGCACTCCAACTATCACTCTAACTGCCTCAACTACTCGTGCGGCTGACCATGTTTTTGAGCTTCTCAAACGCCCAAATCAAGAGGAGACAGTAGGAAGGGATGAGTTATATTTAGGCTACGAGCAATGGTTGCCTAGCCCACCAAAGGTACAGAAGCCTAGTTCCCCAAGGATACAGAAACCTAGTCCAATAAAGGTGCAGAAGCCTCAGACTGTACTGAATGCAGCGGCATTAGCTTACCTTGGTGACTGCATATATGAGGTCCTAACCTTCCTATATCACTTACTATCATTATTTCTGATGCCGCTATATGCTCGCAGGCACTTTTTGTTTCCCCCCTTGAGTATTGAAGAATGCAATGACCGTGTGAAGGCAGTTGTACGTTGTGAAGCACAGGATGCATTGCTCCAGAAACTAATGAAGGACAAATGTTTAACAGAAGAAGAGAGGAATGTTCTTCAGTGGGGAAAGAACATAAGTTCAGCTAAGACACGAACCAAAAAGCATGCTGGTGCAGCAGTCTATAACAGAGCATCTTCACTGGAGACATTAGTTGGACATTTCTATCTGACGGATATGAAACGTTTGGAAGAAGTAATGGTGAGGTTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_3

Protein Analysis

242

Amino Acids

27.6

Weight (kDa)

9.39

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_3 PF00636 115 - 230 1.5e-14 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 155, 341, 419
AclI AACGTT 1 cut(s) 697
AcuI CTGAAG 1 cut(s) 557
AfaI GTAC 3 cut(s) 264, 330, 495
AfiI CCNNNNNNNGG 2 cut(s) 259, 484
AgsI TTSAA 1 cut(s) 464
AluBI AGCT 5 cut(s) 47, 111, 175, 350, 599
AluI AGCT 5 cut(s) 47, 111, 175, 350, 599
Alw26I GTCTC 2 cut(s) 194, 653
ApeKI GCWGC 2 cut(s) 338, 629
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 371, 727
AvaII GGWCC 1 cut(s) 376
BauI CACGAG 1 cut(s) 150
BbvI GCAGC 2 cut(s) 350, 641
BciVI GTATCC 2 cut(s) 49, 279
BcoDI GTCTC 2 cut(s) 194, 653
BfaI CTAG 3 cut(s) 249, 273, 297
BfuI GTATCC 2 cut(s) 49, 279
BisI GCNGC 5 cut(s) 156, 339, 342, 419, 630
BlsI GCNGC 5 cut(s) 157, 340, 343, 420, 631
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 376
BmsI GCATC 3 cut(s) 405, 502, 656
BpmI CTGGAG 3 cut(s) 97, 506, 677
BpuEI CTTGAG 1 cut(s) 475
BsaJI CCNNGG 2 cut(s) 281, 355
BsaWI WCCGGW 1 cut(s) 81
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 484
Bse1I ACTGG 1 cut(s) 660
Bse3DI GCAATG 3 cut(s) 245, 478, 515
BseDI CCNNGG 2 cut(s) 281, 355
BseGI GGATG 2 cut(s) 220, 517
BseLI CCNNNNNNNGG 2 cut(s) 259, 484
BseMI GCAATG 3 cut(s) 245, 478, 515
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 660
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 2 cut(s) 350, 641
BsgI GTGCAG 2 cut(s) 332, 648
BsiSI CCGG 1 cut(s) 82
BslI CCNNNNNNNGG 2 cut(s) 259, 484
BsmAI GTCTC 2 cut(s) 194, 653
BsmI GAATGC 2 cut(s) 340, 473
BspACI CCGC 3 cut(s) 155, 341, 419
BspCNI CTCAG 1 cut(s) 334
BsrDI GCAATG 3 cut(s) 245, 478, 515
BsrI ACTGG 1 cut(s) 660
BssECI CCNNGG 2 cut(s) 281, 355
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 2 cut(s) 281, 355
Bst2BI CACGAG 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 205, 328, 479
Bst6I CTCTTC 1 cut(s) 552
BstAPI GCANNNNNTGC 2 cut(s) 244, 512
BstC8I GCNNGC 4 cut(s) 6, 430, 434, 621
BstDEI CTNAG 2 cut(s) 321, 600
BstF5I GGATG 2 cut(s) 220, 517
BstMAI GTCTC 2 cut(s) 194, 653
BstMWI GCNNNNNNNGC 5 cut(s) 14, 244, 347, 512, 629
BstNSI RCATGY 2 cut(s) 8, 623
BstV1I GCAGC 2 cut(s) 350, 641
BsuI GTATCC 2 cut(s) 49, 279
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 2 cut(s) 220, 517
BtsIMutI CAGTG 2 cut(s) 581, 653
Cac8I GCNNGC 4 cut(s) 6, 430, 434, 621
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 3 cut(s) 263, 329, 494
CviAII CATG 3 cut(s) 5, 164, 620
CviQI GTAC 3 cut(s) 263, 329, 494
DdeI CTNAG 2 cut(s) 321, 600
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco130I CCWWGG 2 cut(s) 281, 355
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 281, 355
EcoT22I ATGCAT 2 cut(s) 6, 517
ErhI CCWWGG 2 cut(s) 281, 355
FaeI CATG 3 cut(s) 8, 167, 623
FatI CATG 3 cut(s) 4, 163, 619
Fnu4HI GCNGC 5 cut(s) 156, 339, 342, 419, 630
FokI GGATG 2 cut(s) 227, 524
Fsp4HI GCNGC 5 cut(s) 156, 339, 342, 419, 630
FspBI CTAG 3 cut(s) 249, 273, 297
GluI GCNGC 5 cut(s) 156, 339, 342, 419, 630
GsuI CTGGAG 3 cut(s) 97, 506, 677
HapII CCGG 1 cut(s) 82
Hin1II CATG 3 cut(s) 8, 167, 623
HindIII AAGCTT 1 cut(s) 45
HinfI GANTC 1 cut(s) 69
HpaII CCGG 1 cut(s) 82
HphI GGTGA 2 cut(s) 371, 727
Hpy188I TCNGA 3 cut(s) 324, 414, 684
Hpy188III TCNNGA 2 cut(s) 194, 523
HpyAV CCTTC 4 cut(s) 204, 394, 478, 529
HpyCH4III ACNGT 3 cut(s) 205, 328, 479
HpyCH4IV ACGT 2 cut(s) 496, 697
HpyCH4V TGCA 7 cut(s) 4, 313, 338, 366, 471, 515, 629
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 244, 347, 512, 629
HpyF3I CTNAG 2 cut(s) 321, 600
HpySE526I ACGT 2 cut(s) 496, 697
Hsp92II CATG 3 cut(s) 8, 167, 623
LmnI GCTCC 3 cut(s) 94, 116, 525
LpnPI CCDG 7 cut(s) 95, 127, 418, 494, 536, 609, 641
Lsp1109I GCAGC 2 cut(s) 350, 641
LweI GCATC 3 cut(s) 405, 502, 656
MaeI CTAG 3 cut(s) 249, 273, 297
MaeII ACGT 2 cut(s) 496, 697
MaeIII GTNAC 1 cut(s) 359
MboII GAAGA 6 cut(s) 476, 563, 566, 569, 642, 716
MlyI GAGTC 1 cut(s) 63
MmeI TCCRAC 2 cut(s) 146, 649
MnlI CCTC 6 cut(s) 151, 190, 330, 367, 555, 711
Mph1103I ATGCAT 2 cut(s) 6, 517
MseI TTAA 1 cut(s) 548
MslI CAYNNNNRTG 1 cut(s) 624
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 1 cut(s) 82
Mva1269I GAATGC 2 cut(s) 340, 473
MwoI GCNNNNNNNGC 5 cut(s) 14, 244, 347, 512, 629
NlaIII CATG 3 cut(s) 8, 167, 623
NmuCI GTSAC 1 cut(s) 359
NsiI ATGCAT 2 cut(s) 6, 517
NspI RCATGY 2 cut(s) 8, 623
PaeI GCATGC 2 cut(s) 8, 623
PctI GAATGC 2 cut(s) 340, 473
PkrI GCNGC 5 cut(s) 157, 340, 343, 420, 631
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
PpuMI RGGWCCY 1 cut(s) 376
Psp1406I AACGTT 1 cut(s) 697
Psp5II RGGWCCY 1 cut(s) 376
PspPI GGNCC 1 cut(s) 376
PspPPI RGGWCCY 1 cut(s) 376
RsaI GTAC 3 cut(s) 264, 330, 495
RsaNI GTAC 3 cut(s) 263, 329, 494
RseI CAYNNNNRTG 1 cut(s) 624
SaqAI TTAA 1 cut(s) 548
SatI GCNGC 5 cut(s) 156, 339, 342, 419, 630
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 63
SfaNI GCATC 3 cut(s) 405, 502, 656
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 624
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
SphI GCATGC 2 cut(s) 8, 623
SsiI CCGC 3 cut(s) 155, 341, 419
SspMI CTAG 3 cut(s) 249, 273, 297
StyI CCWWGG 2 cut(s) 281, 355
TaaI ACNGT 3 cut(s) 205, 328, 479
TaiI ACGT 2 cut(s) 499, 700
TatI WGTACW 1 cut(s) 328
TauI GCSGC 3 cut(s) 158, 344, 421
Tru1I TTAA 1 cut(s) 548
Tru9I TTAA 1 cut(s) 548
TscAI CASTG 2 cut(s) 581, 660
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 2 cut(s) 338, 629
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 3 cut(s) 39, 548, 707
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 2 cut(s) 581, 660
VpaK11BI GGWCC 1 cut(s) 376
XceI RCATGY 2 cut(s) 8, 623
XspI CTAG 3 cut(s) 249, 273, 297
Zsp2I ATGCAT 2 cut(s) 6, 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.