AT1G60860

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007229
Physical Location & Seq
Reverse (-)
22400857 .. 22407824
6968 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60860.2

Sequence Viewer

Length: 2331 bp
ATGGCGGGTTTTATCAATCTTGAGGATTCCCCAATGTTTCAAAAACAGGTCTTCTCCTTGGAAGGGACAAGTGATGAGCTTAAAGATCGTTGTCAGAAGTTATACAAAGGTGTTAAGAAGTTCATGGGAGCTTTAGGAGAGGCATCTACTGGAGTTAGTGCCTTTGCAGATTCTCTTGAAGAGTTTGGTGCTGGACATGATGATCCAGTCAGTGTTTCAATTGGAGGTCCAGTAATATCTAAGTTCATAAATACACTTCGAGAGCTCTCCTCTTATAAGGAGTTTCTTCGCTCACAGGTGGAGCATGTATTACTTGAGAGGCTGACAAACTTTATGACTGTTGATCTACAAGAAGCAAAGGAGTCTCGGCGTCGGTTCGATAAAGCTGTCCATTCTTATGATCAGGCACGCGAAAAGTTTGTGTCGCTAAAGAAGAATACCAGAGGAGACATTGTTGCTGAGTTGGAAGAGGATTTGGAGAACTCGAAGTCAGCATTTGAGAAAAGTCGTTTCAATCTAGTAAATTCACTGATGACCATTGAAGCAAAAAAGAAGTACGAGTTCTTGGAATCTATAAGTGCAATTATGGATTCCCATTTCAAATATTTTAAGCTTGGTTATGATCTATTAAGTCAATTGGAGCCTTACATCCATCAGGTATTGACATACGCACAACAGTCAAAAGAACAGTCTAAGATTGAACAAGATCGATTTGCACAACGCATTCAAGAATTCAGGACTCAATCTGAATTAGACAGCCAACAGGCCTCTGCTAAAGCAGATCCCTCCGATGTTGGTGGAAACCATGTTTATAGAGCTATCCCCCGTAAAAACGTAGAAGCAAACTCAGTTTCAACAGCAGACAAGGAAGTAACTAAACAAGGGTATCTATTAAAGCGATCAGCTAGTTTGAGAGCTGATTGGAAAAGGAGATTCTTTGTGCTTGACAACCATGGATCCCTATACTATTACAGAAACACCGGTAATAAATCAGCGAAATCTCAGCATTACTACAGTGGTTTAGGAGAGCATAGTAGCGGCGTCTTTGGGAGATTTCGTACAAGACACAATCGATCAGCTTCACAGGGAAGCCTAGATTGCAATATGATCGATCTCCGTACTTCACTGATAAAATTGGACGCAGAGGACACAGATCTTCGACTCTGTTTTAGAATTATATCTCCTCAAAAGACTTACACACTACAGGCTGAAAATGGAGCTGATAGGATGGATTGGGTAAATAAGATCACAGCAGCTATAACAATACGTCTGAATTCTCATTTTCTGCAGCAGTCACCAGCAAGATACTTGGATAAAAAGAATACTAGTTCTGGTCCTGCCACTGAAAACCTCACCCTGAATCAAAAAGAAGATTATAATCAAAGACTAAATGTGGGGGATGACGTCCTTACAATACTCAGAGAAATTCCTGGAAATAACACTTGTGCAGAATGCAATGCACCTGACCCCGATTGGGCATCATTGAATCTTGGAGTTTTGATGTGCATTGAATGCTCTGGTGTTCACAGGAATCTCGGCGTTCATATATCCAAGGTGAGATCGCTCACATTGGATGTGAAAGTATGGGAGCCTACAATCTTGGACTTATTTCGGAACTTAGGCAACGGATACTGTAATTCAGTCTGGGAGGAGCTACTTCATCACCTTGACGATGACAGTGAAAAGGGATCGACAGACACACTTGCATCAGTTTCGAAACCATCTTCAGAAGATTGGTTTACTCTGAAGGAGAAATATATTAACGGAAAGTACCTGGAGAAAGCACTAGTTGTCAAAGATGAAAGAGAAGCAAATTCTACTGCCTCAAGTAGAATATGGGAAGCGGTTCAAAGCAGGAACATAAGAGACATCTACCGACTCATTGTAAAAGCAGATGCAAATATCATCAATACCAAGTTCGATGATATTACTGACCTTGATGTATATCACCACCACCATGTTGATGCACCAGATGAAGTAAAGAAAAGGCACGATCCAAATGCGTGTCAGAGAATCAAGAATTCGAACGAAGCAAGAAACTGTCTTCAAGGCTGCTCATTGTTGCATGTGGCGTGTCAAAGCGGTGACCCAATCTTGCTTGAACTGCTGTTGCAGTTCGGGGCTGACATAAACATGAGAGATTACCACGGACGAACTCCATTACACCATTGCATCGCATCAGGGAACAACGCATTTGCAAAGGTTTTGCTGAGAAGAGGTGCTCGTCCATCAATAGAGGATGGTGGAGGATTAAGTGTGTTGGAAAGGGCAATGGAGATGGGAGCCATAACCGATGAAGAGCTCTTCCTTCTATTAGCAGAGTGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

776

Amino Acids

87.8

Weight (kDa)

6.21

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 4 - 124 1.3e-18 BAR domain of APPL family
PH PF00169 180 - 309 6.2e-13 PH domain
ArfGap PF01412 358 - 453 4e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 554 - 636 5.2e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 569 - 612 8.8e-06 Ankyrin repeats (many copies)
Ank PF00023 573 - 603 3.6e-06 Ankyrin repeat
Ank_3 PF13606 573 - 601 1e-05 Ankyrin repeat
Ank_4 PF13637 577 - 626 1.4e-08 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 276, 1377
AatII GACGTC 1 cut(s) 1407
AccII CGCG 1 cut(s) 411
AciI CCGC 4 cut(s) 5, 1038, 1844, 2082
AclWI GGATC 6 cut(s) 197, 776, 951, 964, 1696, 1988
AcsI RAATTY 6 cut(s) 523, 731, 1273, 1425, 1813, 2020
AcuI CTGAAG 2 cut(s) 1710, 1766
AcyI GRCGYC 3 cut(s) 370, 1041, 1404
AfaI GTAC 4 cut(s) 557, 1060, 1120, 1772
AfiI CCNNNNNNNGG 2 cut(s) 63, 1474
AgeI ACCGGT 1 cut(s) 980
AhlI ACTAGT 2 cut(s) 1325, 1786
AjnI CCWGG 2 cut(s) 1429, 1773
Alw21I GWGCWC 3 cut(s) 267, 2224, 2304
Alw26I GTCTC 3 cut(s) 369, 441, 1860
AlwI GGATC 6 cut(s) 197, 776, 951, 964, 1696, 1988
AoxI GGCC 1 cut(s) 765
ApeKI GCWGC 3 cut(s) 1253, 1288, 2052
ApoI RAATTY 6 cut(s) 523, 731, 1273, 1425, 1813, 2020
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AsiGI ACCGGT 1 cut(s) 980
Asp700I GAANNNNTTC 1 cut(s) 1845
AspS9I GGNCC 2 cut(s) 227, 1334
AsuHPI GGTGA 6 cut(s) 1287, 1345, 1567, 1655, 1940, 2096
AsuII TTCGAA 2 cut(s) 1715, 2024
AvaII GGWCC 2 cut(s) 227, 1334
BaeI ACNNNNGTAYC 2 cut(s) 869, 902
BamHI GGATCC 1 cut(s) 956
BanII GRGCYC 2 cut(s) 267, 2304
BbsI GAAGAC 2 cut(s) 43, 2036
Bbv12I GWGCWC 3 cut(s) 267, 2224, 2304
BbvI GCAGC 3 cut(s) 1265, 1300, 2039
BccI CCATC 6 cut(s) 660, 1222, 1729, 2234, 2236, 2272
BcgI CGANNNNNNTGC 6 cut(s) 1090, 1124, 1695, 1729, 1982, 2016
BciT130I CCWGG 2 cut(s) 1431, 1775
BciVI GTATCC 1 cut(s) 1622
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 3 cut(s) 369, 441, 1860
BcuI ACTAGT 2 cut(s) 1325, 1786
BfaI CTAG 5 cut(s) 518, 906, 1094, 1326, 1787
BfmI CTRYAG 3 cut(s) 1012, 1202, 1286
BfuI GTATCC 1 cut(s) 1622
BglII AGATCT 1 cut(s) 1153
BisI GCNGC 4 cut(s) 1039, 1254, 1289, 2053
BlsI GCNGC 4 cut(s) 1040, 1255, 1290, 2054
Bme1390I CCNGG 2 cut(s) 1431, 1775
Bme18I GGWCC 2 cut(s) 227, 1334
BmgT120I GGNCC 2 cut(s) 227, 1334
BmiI GGNNCC 4 cut(s) 642, 958, 1590, 2284
BmrFI CCNGG 2 cut(s) 1431, 1775
BmsI GCATC 7 cut(s) 152, 1487, 1715, 1885, 1954, 2181, 2186
BpiI GAAGAC 2 cut(s) 43, 2036
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BpmI CTGGAG 2 cut(s) 171, 1796
Bpu14I TTCGAA 2 cut(s) 1715, 2024
BpuEI CTTGAG 3 cut(s) 41, 335, 1810
Bsa29I ATCGAT 3 cut(s) 709, 1072, 1110
BsaBI GATNNNNATC 2 cut(s) 1377, 1944
BsaHI GRCGYC 3 cut(s) 370, 1041, 1404
BsaJI CCNNGG 4 cut(s) 57, 952, 1551, 2146
BsaWI WCCGGW 1 cut(s) 980
BsaXI ACNNNNNCTCC 6 cut(s) 216, 246, 438, 468, 1042, 1072
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 1474
Bse118I RCCGGY 1 cut(s) 980
Bse1I ACTGG 3 cut(s) 154, 206, 230
Bse3DI GCAATG 3 cut(s) 1462, 2167, 2277
Bse8I GATNNNNATC 2 cut(s) 1377, 1944
BseBI CCWGG 2 cut(s) 1431, 1775
BseCI ATCGAT 3 cut(s) 709, 1072, 1110
BseDI CCNNGG 4 cut(s) 57, 952, 1551, 2146
BseGI GGATG 5 cut(s) 648, 1233, 1405, 1579, 2245
BseJI GATNNNNATC 2 cut(s) 1377, 1944
BseLI CCNNNNNNNGG 2 cut(s) 63, 1474
BseMI GCAATG 3 cut(s) 1462, 2167, 2277
BseMII CTCAG 5 cut(s) 450, 861, 1016, 1432, 2201
BseNI ACTGG 3 cut(s) 154, 206, 230
BseRI GAGGAG 4 cut(s) 259, 459, 1173, 1664
BseXI GCAGC 3 cut(s) 1265, 1300, 2039
BsgI GTGCAG 1 cut(s) 1467
Bsh1236I CGCG 1 cut(s) 411
BshFI GGCC 1 cut(s) 767
BshTI ACCGGT 1 cut(s) 980
BshVI ATCGAT 3 cut(s) 709, 1072, 1110
BsiHKAI GWGCWC 3 cut(s) 267, 2224, 2304
BsiSI CCGG 1 cut(s) 981
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 63, 1474
BsmAI GTCTC 3 cut(s) 369, 441, 1860
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 3 cut(s) 723, 1457, 1517
BsnI GGCC 1 cut(s) 767
Bsp119I TTCGAA 2 cut(s) 1715, 2024
Bsp1286I GDGCHC 3 cut(s) 267, 2224, 2304
Bsp19I CCATGG 1 cut(s) 952
BspACI CCGC 4 cut(s) 5, 1038, 1844, 2082
BspANI GGCC 1 cut(s) 767
BspCNI CTCAG 5 cut(s) 451, 860, 1015, 1431, 2202
BspDI ATCGAT 3 cut(s) 709, 1072, 1110
BspFNI CGCG 1 cut(s) 411
BspLI GGNNCC 4 cut(s) 642, 958, 1590, 2284
BspMAI CTGCAG 1 cut(s) 1290
BspPI GGATC 6 cut(s) 197, 776, 951, 964, 1696, 1988
BspQI GCTCTTC 2 cut(s) 2292, 2309
BspT104I TTCGAA 2 cut(s) 1715, 2024
BsrDI GCAATG 3 cut(s) 1462, 2167, 2277
BsrFI RCCGGY 1 cut(s) 980
BsrI ACTGG 3 cut(s) 154, 206, 230
BssAI RCCGGY 1 cut(s) 980
BssECI CCNNGG 4 cut(s) 57, 952, 1551, 2146
BssNI GRCGYC 3 cut(s) 370, 1041, 1404
BssT1I CCWWGG 3 cut(s) 57, 952, 1551
Bst2UI CCWGG 2 cut(s) 1431, 1775
Bst4CI ACNGT 7 cut(s) 340, 678, 690, 1016, 1634, 1679, 2042
Bst6I CTCTTC 5 cut(s) 174, 462, 2209, 2292, 2309
BstACI GRCGYC 3 cut(s) 370, 1041, 1404
BstAPI GCANNNNNTGC 1 cut(s) 1512
BstBI TTCGAA 2 cut(s) 1715, 2024
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 8 cut(s) 240, 459, 693, 847, 1002, 1418, 1618, 2210
BstDSI CCRYGG 2 cut(s) 952, 2146
BstEII GGTNACC 1 cut(s) 2084
BstF5I GGATG 5 cut(s) 648, 1233, 1405, 1579, 2245
BstFNI CGCG 1 cut(s) 411
BstMAI GTCTC 3 cut(s) 369, 441, 1860
BstMWI GCNNNNNNNGC 3 cut(s) 1098, 1512, 2104
BstNI CCWGG 2 cut(s) 1431, 1775
BstNSI RCATGY 2 cut(s) 308, 2069
BstPI GGTNACC 1 cut(s) 2084
BstSCI CCNGG 2 cut(s) 1429, 1773
BstSFI CTRYAG 3 cut(s) 1012, 1202, 1286
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 3 cut(s) 1265, 1300, 2039
BstV2I GAAGAC 2 cut(s) 43, 2036
BstX2I RGATCY 3 cut(s) 781, 956, 1153
BstYI RGATCY 3 cut(s) 781, 956, 1153
Bsu15I ATCGAT 3 cut(s) 709, 1072, 1110
BsuI GTATCC 1 cut(s) 1622
BsuRI GGCC 1 cut(s) 767
BsuTUI ATCGAT 3 cut(s) 709, 1072, 1110
BtgI CCRYGG 2 cut(s) 952, 2146
BtgZI GCGATG 1 cut(s) 2158
BtsCI GGATG 5 cut(s) 648, 1233, 1405, 1579, 2245
BtsIMutI CAGTG 6 cut(s) 217, 527, 1021, 1124, 1341, 1684
Cac8I GCNNGC 1 cut(s) 409
Cfr10I RCCGGY 1 cut(s) 980
Cfr13I GGNCC 2 cut(s) 227, 1334
ClaI ATCGAT 3 cut(s) 709, 1072, 1110
CseI GACGC 3 cut(s) 359, 1030, 1148
Csp6I GTAC 4 cut(s) 556, 1059, 1119, 1771
CspAI ACCGGT 1 cut(s) 980
CviAII CATG 8 cut(s) 124, 197, 305, 806, 953, 1958, 2066, 2134
CviQI GTAC 4 cut(s) 556, 1059, 1119, 1771
DdeI CTNAG 8 cut(s) 240, 459, 693, 847, 1002, 1418, 1618, 2210
Eam1104I CTCTTC 5 cut(s) 174, 462, 2209, 2292, 2309
EarI CTCTTC 5 cut(s) 174, 462, 2209, 2292, 2309
Ecl136II GAGCTC 2 cut(s) 265, 2302
Eco130I CCWWGG 3 cut(s) 57, 952, 1551
Eco147I AGGCCT 1 cut(s) 767
Eco24I GRGCYC 2 cut(s) 267, 2304
Eco47I GGWCC 2 cut(s) 227, 1334
Eco53kI GAGCTC 2 cut(s) 265, 2302
Eco57I CTGAAG 2 cut(s) 1710, 1766
Eco91I GGTNACC 1 cut(s) 2084
EcoICRI GAGCTC 2 cut(s) 265, 2302
EcoO65I GGTNACC 1 cut(s) 2084
EcoRI GAATTC 3 cut(s) 731, 1273, 2020
EcoRII CCWGG 2 cut(s) 1429, 1773
EcoT14I CCWWGG 3 cut(s) 57, 952, 1551
EcoT38I GRGCYC 2 cut(s) 267, 2304
ErhI CCWWGG 3 cut(s) 57, 952, 1551
FaeI CATG 8 cut(s) 127, 200, 308, 809, 956, 1961, 2069, 2137
FaqI GGGAC 1 cut(s) 79
FatI CATG 8 cut(s) 123, 196, 304, 805, 952, 1957, 2065, 2133
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 4 cut(s) 1039, 1254, 1289, 2053
FokI GGATG 5 cut(s) 635, 1240, 1412, 1586, 2252
FriOI GRGCYC 2 cut(s) 267, 2304
Fsp4HI GCNGC 4 cut(s) 1039, 1254, 1289, 2053
FspBI CTAG 5 cut(s) 518, 906, 1094, 1326, 1787
GluI GCNGC 4 cut(s) 1039, 1254, 1289, 2053
GsuI CTGGAG 2 cut(s) 171, 1796
HaeIII GGCC 1 cut(s) 767
HapII CCGG 1 cut(s) 981
HgaI GACGC 3 cut(s) 359, 1030, 1148
Hin1I GRCGYC 3 cut(s) 370, 1041, 1404
Hin1II CATG 8 cut(s) 127, 200, 308, 809, 956, 1961, 2069, 2137
HindIII AAGCTT 1 cut(s) 611
HpaII CCGG 1 cut(s) 981
HphI GGTGA 6 cut(s) 1287, 1345, 1567, 1655, 1940, 2096
Hpy166II GTNNAC 2 cut(s) 1525, 1740
Hpy188I TCNGA 9 cut(s) 96, 748, 790, 1272, 1421, 1614, 1729, 1746, 2010
Hpy188III TCNNGA 6 cut(s) 20, 176, 260, 728, 736, 2017
Hpy8I GTNNAC 2 cut(s) 1525, 1740
Hpy99I CGWCG 1 cut(s) 375
HpyAV CCTTC 3 cut(s) 56, 1741, 2318
HpyCH4III ACNGT 7 cut(s) 340, 678, 690, 1016, 1634, 1679, 2042
HpyCH4IV ACGT 3 cut(s) 834, 1267, 1404
HpyF10VI GCNNNNNNNGC 3 cut(s) 1098, 1512, 2104
HpyF3I CTNAG 8 cut(s) 240, 459, 693, 847, 1002, 1418, 1618, 2210
HpySE526I ACGT 3 cut(s) 834, 1267, 1404
Hsp92I GRCGYC 3 cut(s) 370, 1041, 1404
Hsp92II CATG 8 cut(s) 127, 200, 308, 809, 956, 1961, 2069, 2137
Ksp22I TGATCA 1 cut(s) 400
LguI GCTCTTC 2 cut(s) 2292, 2309
LmnI GCTCC 7 cut(s) 128, 301, 640, 1217, 1588, 1651, 2282
Lsp1109I GCAGC 3 cut(s) 1265, 1300, 2039
LweI GCATC 7 cut(s) 152, 1487, 1715, 1885, 1954, 2181, 2186
MaeI CTAG 5 cut(s) 518, 906, 1094, 1326, 1787
MaeII ACGT 3 cut(s) 834, 1267, 1404
MaeIII GTNAC 3 cut(s) 871, 1293, 2084
MfeI CAATTG 2 cut(s) 219, 635
MflI RGATCY 3 cut(s) 781, 956, 1153
MhlI GDGCHC 3 cut(s) 267, 2224, 2304
MlyI GAGTC 4 cut(s) 371, 733, 1155, 1872
MmeI TCCRAC 2 cut(s) 444, 2241
MroXI GAANNNNTTC 1 cut(s) 1845
MseI TTAA 7 cut(s) 81, 114, 609, 629, 893, 1761, 2252
MslI CAYNNNNRTG 4 cut(s) 396, 1956, 1962, 2132
MspI CCGG 1 cut(s) 981
MspR9I CCNGG 2 cut(s) 1431, 1775
MunI CAATTG 2 cut(s) 219, 635
Mva1269I GAATGC 3 cut(s) 723, 1457, 1517
MvaI CCWGG 2 cut(s) 1431, 1775
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 3 cut(s) 1098, 1512, 2104
NcoI CCATGG 1 cut(s) 952
NlaIII CATG 8 cut(s) 127, 200, 308, 809, 956, 1961, 2069, 2137
NlaIV GGNNCC 4 cut(s) 642, 958, 1590, 2284
NmeAIII GCCGAG 2 cut(s) 346, 1515
NmuCI GTSAC 2 cut(s) 1293, 2084
NspI RCATGY 2 cut(s) 308, 2069
NspV TTCGAA 2 cut(s) 1715, 2024
PceI AGGCCT 1 cut(s) 767
PciSI GCTCTTC 2 cut(s) 2292, 2309
PctI GAATGC 3 cut(s) 723, 1457, 1517
PdmI GAANNNNTTC 1 cut(s) 1845
PfeI GAWTC 9 cut(s) 26, 170, 569, 590, 933, 1360, 1486, 1531, 2013
PfoI TCCNGGA 1 cut(s) 1429
PinAI ACCGGT 1 cut(s) 980
PkrI GCNGC 4 cut(s) 1040, 1255, 1290, 2054
PleI GAGTC 4 cut(s) 370, 733, 1155, 1872
PpsI GAGTC 4 cut(s) 370, 733, 1155, 1872
PsiI TTATAA 2 cut(s) 276, 1377
Psp124BI GAGCTC 2 cut(s) 267, 2304
Psp6I CCWGG 2 cut(s) 1429, 1773
PspEI GGTNACC 1 cut(s) 2084
PspGI CCWGG 2 cut(s) 1429, 1773
PspN4I GGNNCC 4 cut(s) 642, 958, 1590, 2284
PspPI GGNCC 2 cut(s) 227, 1334
PstI CTGCAG 1 cut(s) 1290
PsuI RGATCY 3 cut(s) 781, 956, 1153
RsaI GTAC 4 cut(s) 557, 1060, 1120, 1772
RsaNI GTAC 4 cut(s) 556, 1059, 1119, 1771
RseI CAYNNNNRTG 4 cut(s) 396, 1956, 1962, 2132
SacI GAGCTC 2 cut(s) 267, 2304
SapI GCTCTTC 2 cut(s) 2292, 2309
SaqAI TTAA 7 cut(s) 81, 114, 609, 629, 893, 1761, 2252
SatI GCNGC 4 cut(s) 1039, 1254, 1289, 2053
Sau96I GGNCC 2 cut(s) 227, 1334
SchI GAGTC 4 cut(s) 371, 733, 1155, 1872
ScrFI CCNGG 2 cut(s) 1431, 1775
SduI GDGCHC 3 cut(s) 267, 2224, 2304
SfaNI GCATC 7 cut(s) 152, 1487, 1715, 1885, 1954, 2181, 2186
SfcI CTRYAG 3 cut(s) 1012, 1202, 1286
SfuI TTCGAA 2 cut(s) 1715, 2024
SinI GGWCC 2 cut(s) 227, 1334
SmiMI CAYNNNNRTG 4 cut(s) 396, 1956, 1962, 2132
SmlI CTYRAG 3 cut(s) 20, 314, 1825
SmoI CTYRAG 3 cut(s) 20, 314, 1825
SpeI ACTAGT 2 cut(s) 1325, 1786
SseBI AGGCCT 1 cut(s) 767
SsiI CCGC 4 cut(s) 5, 1038, 1844, 2082
SspI AATATT 1 cut(s) 605
SspMI CTAG 5 cut(s) 518, 906, 1094, 1326, 1787
SstI GAGCTC 2 cut(s) 267, 2304
StuI AGGCCT 1 cut(s) 767
StyD4I CCNGG 2 cut(s) 1429, 1773
StyI CCWWGG 3 cut(s) 57, 952, 1551
TaaI ACNGT 7 cut(s) 340, 678, 690, 1016, 1634, 1679, 2042
TaiI ACGT 3 cut(s) 837, 1270, 1407
TauI GCSGC 1 cut(s) 1041
TfiI GAWTC 9 cut(s) 26, 170, 569, 590, 933, 1360, 1486, 1531, 2013
Tru1I TTAA 7 cut(s) 81, 114, 609, 629, 893, 1761, 2252
Tru9I TTAA 7 cut(s) 81, 114, 609, 629, 893, 1761, 2252
TscAI CASTG 6 cut(s) 217, 534, 1021, 1131, 1348, 1684
TseFI GTSAC 2 cut(s) 1293, 2084
TseI GCWGC 3 cut(s) 1253, 1288, 2052
Tsp45I GTSAC 2 cut(s) 1293, 2084
TspDTI ATGAA 7 cut(s) 112, 235, 1532, 1649, 1815, 1989, 2310
TspGWI ACGGA 4 cut(s) 1106, 1641, 1779, 2163
TspRI CASTG 6 cut(s) 217, 534, 1021, 1131, 1348, 1684
VpaK11BI GGWCC 2 cut(s) 227, 1334
XapI RAATTY 6 cut(s) 523, 731, 1273, 1425, 1813, 2020
XceI RCATGY 2 cut(s) 308, 2069
XmnI GAANNNNTTC 1 cut(s) 1845
XspI CTAG 5 cut(s) 518, 906, 1094, 1326, 1787
ZraI GACGTC 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.