pycom13g00700

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007229
Physical Location & Seq
Forward (+)
438943 .. 444236
5294 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00700.1

Sequence Viewer

Length: 1455 bp
ATGGCGGCCTTCATCAAGCTCGAGGATTCCCCAATGTTTCAGAAGCAGATATATTCTATGGAACAAACAGCTGATGAGTTGAAAGATCGCTGCCAAAAGTTATACAAAGGGTCTAAGAAGTTTATGGGAGCTCTCGAAGAAGCATGTAATGGAGACACCACTTTTTCGGAATCTTTGGAAGCATTTGGCGGTGGACTTGACGATCCTATTAGCGTATCTGTTGGAGGTCCAGTATTATCCAAATTTGTTTCTGCATTTCAAGAGCTTGCCACTTATAAAGAGCTTCTTCGTACCCAAGTAGAGCATGTTTTAGTTGATCGGTTGATGCACTTTATGACAGTTGATCTGCAAGAAGCAAAGGAATCACGACGTCGCTTTGACAAAGTTATACATGGTTATGATCAGGCACGGGAAAAGTTTGTGTCCTTAAAGAAGAATACACGGGAAGACATTGTTGCAGAACTAGAGGAGGGATATGATTTATTCAGCCAAATGGAGCCATATATTCACCAGGTGTTAACATACGCACAACAATCTAAAGAACAGGCCACAGTTGAACAAGATAAACTTCACAGAAGGATTCAGGCGTATAGGACACAAGCTGAGCTGGACAGTATACGAGCTTCCAGTAATATTGAGCCTCCTGCAGCGGCTGATGGGAATCGCCCCTTTGGTTTAGCTTCATACAAAAACATAGAAGCAATCATGCAGTCCAGTGCAAATGGGAAGGTCCAGACGATCAAGCAAGGATATCTATTGAAACGATCTTCAAGTTTGAGGGGAGATTGGAAGAGGAGGTTTTTTGTACTGAACAGTCATGGGGCTTTATTTTATTACAGAATTAAGGGTACCAAACCCATGAGCGGCTCTCAATCACATCATTTTACGCGCTCACCGGAACATCACAGTGGTGTGTTTGGTAGATTTCGTTCAAGAAATAGGGTAGCAGCATCACTTAATGAAAATGATCTGGGTTGCCGTACTGTTGATTTGCGTACTTCAACAATAAAGATGGATGCAGAGGATACAGATCTTCGACTTTGCTTCAGAATAATATCCCCATTGAAAACTTACACATTGCAGGCTGAAAATGAAGCAGATAGGATTGATTGGACAAACAAAATAACAGGAGCTATTGCATCGCTTTTGAATTTTCAGCTTCTTGATCAGCCACATCCTGGAAGTGTGGATATTGAGAGTTGTAACTCTTCTCTTGGCACTGATGATATGCGTCGAGTAAAAAGCACTGAAAGTTTAGGAAATACCATAAAACTCAACAGAGTAGATTCTGTGTCTAGTATTCTCAGGAGAATTCCTGGAAATGATCTTTGTGCAGAGTGCAGTGCTCCTGAGCCTGACTGGGCATCTCTTAACCTTGGCATACTGTTATGCATCGAATGTTCTGGTGTTCATCGGAATCTTGGTGTACACATTTCAAAGGTGAGATCGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

485

Amino Acids

54.52

Weight (kDa)

7.61

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 7 - 154 2.7e-21 BAR domain of APPL family
PH PF00169 247 - 379 4.5e-11 PH domain
ArfGap PF01412 433 - 484 9.6e-22 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
AatII GACGTC 1 cut(s) 373
Acc65I GGTACC 1 cut(s) 848
AccB1I GGYRCC 1 cut(s) 848
AccB7I CCANNNNNTGG 1 cut(s) 1178
AccBSI CCGCTC 1 cut(s) 864
AccI GTMKAC 1 cut(s) 616
AccII CGCG 1 cut(s) 889
AciI CCGC 4 cut(s) 5, 189, 650, 864
AclWI GGATC 1 cut(s) 197
AcsI RAATTY 3 cut(s) 242, 1150, 1311
AcuI CTGAAG 1 cut(s) 1030
AcyI GRCGYC 1 cut(s) 370
AdeI CACNNNGTG 1 cut(s) 514
AfaI GTAC 6 cut(s) 292, 807, 850, 982, 997, 1428
AfiI CCNNNNNNNGG 2 cut(s) 863, 1178
AjnI CCWGG 3 cut(s) 510, 1177, 1315
AleI CACNNNNGTG 1 cut(s) 909
Alw21I GWGCWC 2 cut(s) 133, 1348
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 1 cut(s) 197
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 2 cut(s) 6, 546
ApeKI GCWGC 3 cut(s) 90, 647, 947
ApoI RAATTY 3 cut(s) 242, 1150, 1311
Asp718I GGTACC 1 cut(s) 848
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 2 cut(s) 227, 730
AsuHPI GGTGA 3 cut(s) 500, 885, 1453
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 2 cut(s) 227, 730
BanI GGYRCC 1 cut(s) 848
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 607, 639
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 2 cut(s) 133, 1348
BbvI GCAGC 3 cut(s) 77, 659, 959
BccI CCATC 2 cut(s) 650, 1006
BceAI ACGGC 1 cut(s) 963
BciT130I CCWGG 3 cut(s) 512, 1179, 1317
BciVI GTATCC 1 cut(s) 1018
BclI TGATCA 2 cut(s) 400, 1165
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 2 cut(s) 464, 1296
BfmI CTRYAG 1 cut(s) 645
BfuI GTATCC 1 cut(s) 1018
BglII AGATCT 1 cut(s) 1030
BisI GCNGC 6 cut(s) 6, 91, 648, 651, 865, 948
BlpI GCTNAGC 1 cut(s) 603
BlsI GCNGC 6 cut(s) 7, 92, 649, 652, 866, 949
Bme1390I CCNGG 3 cut(s) 512, 1179, 1317
Bme18I GGWCC 2 cut(s) 227, 730
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 2 cut(s) 227, 730
BmiI GGNNCC 2 cut(s) 498, 850
BmrFI CCNGG 3 cut(s) 512, 1179, 1317
BmrI ACTGGG 1 cut(s) 1369
BmsI GCATC 6 cut(s) 315, 959, 1006, 1148, 1373, 1401
BmuI ACTGGG 1 cut(s) 1369
BpiI GAAGAC 1 cut(s) 453
BplI GAGNNNNNCTC 2 cut(s) 853, 885
Bpu10I CCTNAGC 1 cut(s) 1350
Bpu1102I GCTNAGC 1 cut(s) 603
Bsa29I ATCGAT 1 cut(s) 1448
BsaBI GATNNNNATC 2 cut(s) 660, 1029
BsaHI GRCGYC 1 cut(s) 370
BsaJI CCNNGG 1 cut(s) 1375
BsaWI WCCGGW 1 cut(s) 895
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 2 cut(s) 863, 1178
Bse1I ACTGG 4 cut(s) 230, 627, 714, 1364
Bse3DI GCAATG 1 cut(s) 1076
Bse8I GATNNNNATC 2 cut(s) 660, 1029
BseBI CCWGG 3 cut(s) 512, 1179, 1317
BseCI ATCGAT 1 cut(s) 1448
BseDI CCNNGG 1 cut(s) 1375
BseGI GGATG 2 cut(s) 1021, 1174
BseJI GATNNNNATC 2 cut(s) 660, 1029
BseLI CCNNNNNNNGG 2 cut(s) 863, 1178
BseMI GCAATG 1 cut(s) 1076
BseMII CTCAG 3 cut(s) 594, 1318, 1341
BseNI ACTGG 4 cut(s) 230, 627, 714, 1364
BseRI GAGGAG 2 cut(s) 482, 808
BseXI GCAGC 3 cut(s) 77, 659, 959
BsgI GTGCAG 2 cut(s) 1353, 1360
Bsh1236I CGCG 1 cut(s) 889
BshFI GGCC 2 cut(s) 8, 548
BshNI GGYRCC 1 cut(s) 848
BshVI ATCGAT 1 cut(s) 1448
BsiHKAI GWGCWC 2 cut(s) 133, 1348
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 1 cut(s) 896
BslI CCNNNNNNNGG 2 cut(s) 863, 1178
BsmAI GTCTC 1 cut(s) 147
BsnI GGCC 2 cut(s) 8, 548
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 2 cut(s) 133, 1348
Bsp1407I TGTACA 1 cut(s) 1426
Bsp1720I GCTNAGC 1 cut(s) 603
BspACI CCGC 4 cut(s) 5, 189, 650, 864
BspANI GGCC 2 cut(s) 8, 548
BspCNI CTCAG 3 cut(s) 595, 1317, 1342
BspDI ATCGAT 1 cut(s) 1448
BspFNI CGCG 1 cut(s) 889
BspLI GGNNCC 2 cut(s) 498, 850
BspMAI CTGCAG 1 cut(s) 649
BspPI GGATC 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 848
BsrBI CCGCTC 1 cut(s) 864
BsrDI GCAATG 1 cut(s) 1076
BsrGI TGTACA 1 cut(s) 1426
BsrI ACTGG 4 cut(s) 230, 627, 714, 1364
BssECI CCNNGG 1 cut(s) 1375
BssNAI GTATAC 1 cut(s) 617
BssNI GRCGYC 1 cut(s) 370
BssT1I CCWWGG 1 cut(s) 1375
Bst1107I GTATAC 1 cut(s) 617
Bst2UI CCWGG 3 cut(s) 512, 1179, 1317
Bst4CI ACNGT 7 cut(s) 340, 553, 614, 815, 908, 985, 1386
Bst6I CTCTTC 2 cut(s) 785, 1213
BstACI GRCGYC 1 cut(s) 370
BstAUI TGTACA 1 cut(s) 1426
BstC8I GCNNGC 2 cut(s) 267, 1083
BstDEI CTNAG 4 cut(s) 114, 603, 1304, 1350
BstF5I GGATG 2 cut(s) 1021, 1174
BstFNI CGCG 1 cut(s) 889
BstHHI GCGC 1 cut(s) 891
BstMAI GTCTC 1 cut(s) 147
BstNI CCWGG 3 cut(s) 512, 1179, 1317
BstNSI RCATGY 2 cut(s) 147, 308
BstSCI CCNGG 3 cut(s) 510, 1177, 1315
BstSFI CTRYAG 1 cut(s) 645
BstUI CGCG 1 cut(s) 889
BstV1I GCAGC 3 cut(s) 77, 659, 959
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 1 cut(s) 1030
BstYI RGATCY 1 cut(s) 1030
BstZ17I GTATAC 1 cut(s) 617
Bsu15I ATCGAT 1 cut(s) 1448
BsuI GTATCC 1 cut(s) 1018
BsuRI GGCC 2 cut(s) 8, 548
BsuTUI ATCGAT 1 cut(s) 1448
BtgZI GCGATG 1 cut(s) 1125
BtsCI GGATG 2 cut(s) 1021, 1174
BtsI GCAGTG 1 cut(s) 1348
BtsIMutI CAGTG 5 cut(s) 721, 913, 1218, 1245, 1348
Cac8I GCNNGC 2 cut(s) 267, 1083
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 1 cut(s) 891
Cfr13I GGNCC 2 cut(s) 227, 730
ClaI ATCGAT 1 cut(s) 1448
CseI GACGC 1 cut(s) 1220
CsiI ACCWGGT 1 cut(s) 510
Csp6I GTAC 6 cut(s) 291, 806, 849, 981, 996, 1427
CviAII CATG 6 cut(s) 144, 305, 392, 706, 818, 859
CviQI GTAC 6 cut(s) 291, 806, 849, 981, 996, 1427
DdeI CTNAG 4 cut(s) 114, 603, 1304, 1350
DraIII CACNNNGTG 1 cut(s) 514
Eam1104I CTCTTC 2 cut(s) 785, 1213
EarI CTCTTC 2 cut(s) 785, 1213
Ecl136II GAGCTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 1375
Eco24I GRGCYC 1 cut(s) 133
Eco32I GATATC 1 cut(s) 752
Eco47I GGWCC 2 cut(s) 227, 730
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 1030
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 1311
EcoRII CCWGG 3 cut(s) 510, 1177, 1315
EcoRV GATATC 1 cut(s) 752
EcoT14I CCWWGG 1 cut(s) 1375
EcoT22I ATGCAT 1 cut(s) 1394
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 1375
FaeI CATG 6 cut(s) 147, 308, 395, 709, 821, 862
FalI AAGNNNNNCTT 4 cut(s) 270, 302, 552, 584
FatI CATG 6 cut(s) 143, 304, 391, 705, 817, 858
FbaI TGATCA 2 cut(s) 400, 1165
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 6 cut(s) 6, 91, 648, 651, 865, 948
FokI GGATG 2 cut(s) 1028, 1161
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 6 cut(s) 6, 91, 648, 651, 865, 948
FspBI CTAG 2 cut(s) 464, 1296
GlaI GCGC 1 cut(s) 890
GluI GCNGC 6 cut(s) 6, 91, 648, 651, 865, 948
HaeIII GGCC 2 cut(s) 8, 548
HapII CCGG 1 cut(s) 896
HgaI GACGC 1 cut(s) 1220
HhaI GCGC 1 cut(s) 891
Hin1I GRCGYC 1 cut(s) 370
Hin1II CATG 6 cut(s) 147, 308, 395, 709, 821, 862
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HinfI GANTC 7 cut(s) 26, 170, 362, 580, 661, 1286, 1417
HpaI GTTAAC 1 cut(s) 519
HpaII CCGG 1 cut(s) 896
HphI GGTGA 3 cut(s) 500, 885, 1453
Hpy166II GTNNAC 5 cut(s) 194, 519, 617, 1427, 1429
Hpy188I TCNGA 4 cut(s) 42, 169, 1049, 1416
Hpy188III TCNNGA 8 cut(s) 134, 260, 366, 733, 933, 1163, 1306, 1349
Hpy8I GTNNAC 5 cut(s) 194, 519, 617, 1427, 1429
Hpy99I CGWCG 3 cut(s) 372, 375, 1236
HpyAV CCTTC 3 cut(s) 19, 570, 721
HpyCH4III ACNGT 7 cut(s) 340, 553, 614, 815, 908, 985, 1386
HpyCH4IV ACGT 1 cut(s) 370
HpyF3I CTNAG 4 cut(s) 114, 603, 1304, 1350
HpySE526I ACGT 1 cut(s) 370
Hsp92I GRCGYC 1 cut(s) 370
Hsp92II CATG 6 cut(s) 147, 308, 395, 709, 821, 862
HspAI GCGC 1 cut(s) 889
KpnI GGTACC 1 cut(s) 852
Ksp22I TGATCA 2 cut(s) 400, 1165
KspAI GTTAAC 1 cut(s) 519
LmnI GCTCC 4 cut(s) 128, 496, 1130, 1351
Lsp1109I GCAGC 3 cut(s) 77, 659, 959
LweI GCATC 6 cut(s) 315, 959, 1006, 1148, 1373, 1401
MabI ACCWGGT 1 cut(s) 510
MaeI CTAG 2 cut(s) 464, 1296
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 1 cut(s) 1202
MbiI CCGCTC 1 cut(s) 864
MboII GAAGA 8 cut(s) 149, 278, 445, 458, 759, 802, 1025, 1200
MflI RGATCY 1 cut(s) 1030
MhlI GDGCHC 2 cut(s) 133, 1348
MluCI AATT 4 cut(s) 242, 840, 1150, 1311
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 9 cut(s) 16, 218, 460, 463, 651, 771, 786, 789, 1015
Mph1103I ATGCAT 1 cut(s) 1394
MseI TTAA 5 cut(s) 428, 518, 843, 957, 1371
MslI CAYNNNNRTG 3 cut(s) 396, 906, 909
MspA1I CMGCKG 2 cut(s) 71, 650
MspI CCGG 1 cut(s) 896
MspR9I CCNGG 3 cut(s) 512, 1179, 1317
MvaI CCWGG 3 cut(s) 512, 1179, 1317
MvnI CGCG 1 cut(s) 889
NlaIII CATG 6 cut(s) 147, 308, 395, 709, 821, 862
NlaIV GGNNCC 2 cut(s) 498, 850
NsiI ATGCAT 1 cut(s) 1394
NspI RCATGY 2 cut(s) 147, 308
OliI CACNNNNGTG 1 cut(s) 909
PaeR7I CTCGAG 1 cut(s) 20
PfeI GAWTC 7 cut(s) 26, 170, 362, 580, 661, 1286, 1417
PflMI CCANNNNNTGG 1 cut(s) 1178
PfoI TCCNGGA 2 cut(s) 1177, 1315
PkrI GCNGC 6 cut(s) 7, 92, 649, 652, 866, 949
PsiI TTATAA 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 133
Psp6I CCWGG 3 cut(s) 510, 1177, 1315
PspGI CCWGG 3 cut(s) 510, 1177, 1315
PspN4I GGNNCC 2 cut(s) 498, 850
PspPI GGNCC 2 cut(s) 227, 730
PspXI VCTCGAGB 1 cut(s) 20
PsrI GAACNNNNNNTAC 2 cut(s) 913, 945
PstI CTGCAG 1 cut(s) 649
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 1030
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 6 cut(s) 292, 807, 850, 982, 997, 1428
RsaNI GTAC 6 cut(s) 291, 806, 849, 981, 996, 1427
RseI CAYNNNNRTG 3 cut(s) 396, 906, 909
SacI GAGCTC 1 cut(s) 133
SaqAI TTAA 5 cut(s) 428, 518, 843, 957, 1371
SatI GCNGC 6 cut(s) 6, 91, 648, 651, 865, 948
Sau96I GGNCC 2 cut(s) 227, 730
ScrFI CCNGG 3 cut(s) 512, 1179, 1317
SduI GDGCHC 2 cut(s) 133, 1348
SexAI ACCWGGT 1 cut(s) 510
SfaNI GCATC 6 cut(s) 315, 959, 1006, 1148, 1373, 1401
SfcI CTRYAG 1 cut(s) 645
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 2 cut(s) 227, 730
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 3 cut(s) 396, 906, 909
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 4 cut(s) 242, 840, 1150, 1311
SsiI CCGC 4 cut(s) 5, 189, 650, 864
SspI AATATT 1 cut(s) 634
SspMI CTAG 2 cut(s) 464, 1296
SstI GAGCTC 1 cut(s) 133
StyD4I CCNGG 3 cut(s) 510, 1177, 1315
StyI CCWWGG 1 cut(s) 1375
TaaI ACNGT 7 cut(s) 340, 553, 614, 815, 908, 985, 1386
TaiI ACGT 1 cut(s) 373
TaqI TCGA 6 cut(s) 21, 135, 1036, 1234, 1395, 1448
TasI AATT 4 cut(s) 242, 840, 1150, 1311
TatI WGTACW 2 cut(s) 805, 1426
TauI GCSGC 3 cut(s) 8, 653, 867
TfiI GAWTC 7 cut(s) 26, 170, 362, 580, 661, 1286, 1417
Tru1I TTAA 5 cut(s) 428, 518, 843, 957, 1371
Tru9I TTAA 5 cut(s) 428, 518, 843, 957, 1371
TscAI CASTG 5 cut(s) 721, 913, 1225, 1252, 1348
TseI GCWGC 3 cut(s) 90, 647, 947
TspDTI ATGAA 4 cut(s) 672, 975, 1107, 1400
TspRI CASTG 5 cut(s) 721, 913, 1225, 1252, 1348
Van91I CCANNNNNTGG 1 cut(s) 1178
VpaK11BI GGWCC 2 cut(s) 227, 730
XapI RAATTY 3 cut(s) 242, 1150, 1311
XceI RCATGY 2 cut(s) 147, 308
XhoI CTCGAG 1 cut(s) 20
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 2 cut(s) 464, 1296
ZraI GACGTC 1 cut(s) 371
Zsp2I ATGCAT 1 cut(s) 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.