MD13G1008500.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007229
Physical Location & Seq
Forward (+)
518804 .. 526029
7226 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1008500.v1.1.491

Sequence Viewer

Length: 2376 bp
ATGGCGGCCTTCATCAAGCTCGAGGATTCCCCAATGTTTCAGAAGCAGATATATTCTATGGAACAAACAGCTGATGAGTTGAAAGATCGCTGCCAAAAATTATACAAAGGGTCTAAGAAGTTTATGGGAGCTCTCGAAGAAGCACGTAACGGAGACACCACTTTTTCGGAATCTTTGGAAGCATTTGGCGGTGGACTTGACGATCCTATTAGTGTATCCGTTGGAGGTCCTGTATTATCCAAATTCGTTACTGCATTTCAAGAGCTTGCCACTTATAAAGAGCTTCTTCGTACCCAAGTAGAGCATGTTTTAGTTGATCGGTTGATGCACTTTATGACAGTTGATCTGCAAGAAGCAAAGGAATCACGACGTCGCTTTGACAAAGTTATACATGGTTATGATCAGGCACGGGAAAAGTTTGTGTCCTTAAAGAAGAATACACGGGAAGACATTGTTGCAGAACTAGAGGAGGATCTACAAAATTCAAAATCAGCATTCGAGAAAAGCCGCTTCAACCTAGTAAACTCACTAATGAATATTGAAGCAAAAAAGAAGTATGCGTTTTTGGAATCTATCAGTGCAATCATGGATGCTCATCTGAGATACTTTAAGCTGGGATACGATTTATTCAGCCAAATGGAACCATATATTCACCAGGTGTTAACATACGCACAACAATCTAAAGAACAGGCCACTGTTGAACAAGATAAACTTCACAGAAGGATTCAGGAGTATAGAACACAAGCTGAGCTGGACAGTATACGAGCTTCCAATAATATTGAGCCTCCTGCAGCGGCTGATGGAAATCGCCCCTTTGGTTTAGCTTCATACAAAAACATAGAAGCAATAATGCAGTCCAGTGCAAATGGGAAGGTCCAGACGATCAAGCAAGGATATCTATTGAAACGATCTTCAAGTTTGAGGGGAGATTGGAAGAGGAGGTTTTTCGTACTGAACAGTCATGGGGCTTTATTTTATTACAGAATTAAGGGTACCAAACCCATGGGCGGCTCTCAATCACATCATTTCACGCGCTCACCGGAACATCACAGTGGTGTGTTTGGTAGATTTCGTTCAAGAAATAGGGCTGTAGCATCACTTAATGAAAATGTTCTGGGTTGCCGTACTGTTGATTTGCGTACTTCAACAATAAAGATGGATGCAGAGGATACAGATCTTCGACTTTGCTTCAGAATAATATCCCCATTGAAAACTTACACATTGCAGGCTGAAAATGAAGCAGATAGGATTGATTGGACAAACAAAATAACAGGAGCTATTGCATCGCTTTTGAATTTTCAGCTTCTTGATCAGCCATATCCTGGAAGTGTGGATATTGAGAGTTGTAACTCTTCTCTTGGCACTGATGATATGCGTCGAGTAAAAAGCACTGAAAGTTTAGGAAATACCATAAAACTCGACAGAGTAGATTCTGTGTCTAGTATTCTCAGGAGAATTCCTGGAAATGATCTTTGTGTGGAGTGCAGTGCTCCTGAGCCTGACTGGGCATCTCTTAACCTTGGCATACTGTTATGCATCGAATGTTCTGGTGTTCATCGGAATCTTGGTGTACACATTTCAAAGGTGAGATCTCTAACATTAGATGTCAAGGTTTGGGAACCTACACTTGTGGACTATTTTCGCAACTTGGGTAATGCATATTGTAATTCTATATGGGAGGGAACGCTTCTACTTGAGAATGAAAAACTGGATGGTTCGAAGGGCATGAGAGCTTCAATTTTAAAACCAGGCCCCAAGGATACAATTCAGCATAAGGAAATATACATTCAAGCAAAGTATGTAGAGAAAGTGATGGTTATTAGAGATGTAGCTGTATCGGGCATGCCTGTTCATGCAGCAGATATATGGGAAGCAGTCAAGACTGGTAATTTACAAGAAGTCTACCGTATTATCATGATGTCAGATGTGAATATTGTCGACACCACCTTTGAGAATGTGGTTACTCATGATTTATATCACCATGCTGATGCAAAACACTCGGAGTCGGATTTGAACACAATGGAGATGAAACAGTACGATCCAGTAGCATGTGAGAGAATCAAGGAGTCCAATGAGCCAGGGAACTGTCTTCAAGGTTGTTCATTGTTGCATCTGGCTTGTCACTCTGGCAATCCTATGATGGTTGAATTGTTGCTGCAATTTGGTGCCAGCATGAATTTGCGTGATTTCCATGGAAGGACTCCTTTGCACCACTGTATCGCCAGCGGGAACAACTCATTGGCAAAGTCTCTACTTAGGAGGGGTGCACGCCCGTCAATTCAAGACGGAGGAGGGCATAGTGCATTAGAAAGAGCGATGGGGATGGGAGCAATTACAGATGAGGAGTTGTTCATACTACTTTCTGAACACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

792

Amino Acids

89.14

Weight (kDa)

6.46

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 7 - 238 5.1e-37 BAR domain of APPL family
BAR PF03114 19 - 217 1.1e-12 BAR domain
PH PF00169 295 - 427 2e-10 PH domain
ArfGap PF01412 481 - 605 1.2e-30 Putative GTPase activating protein for Arf
Ank_2 PF12796 689 - 762 8.1e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 695 - 738 3.5e-06 Ankyrin repeats (many copies)
Ank PF00023 699 - 729 1.4e-06 Ankyrin repeat
Ank_4 PF13637 703 - 747 1.8e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 276
AatII GACGTC 1 cut(s) 373
Acc65I GGTACC 1 cut(s) 992
AccB1I GGYRCC 2 cut(s) 992, 2165
AccB7I CCANNNNNTGG 1 cut(s) 1322
AccI GTMKAC 3 cut(s) 760, 1902, 1938
AccII CGCG 1 cut(s) 1033
AciI CCGC 6 cut(s) 5, 189, 508, 794, 1008, 2226
AclWI GGATC 3 cut(s) 197, 480, 2033
AcsI RAATTY 5 cut(s) 242, 481, 1294, 1455, 2176
AcuI CTGAAG 1 cut(s) 1174
AcyI GRCGYC 1 cut(s) 370
AdeI CACNNNGTG 1 cut(s) 658
AfaI GTAC 7 cut(s) 292, 951, 994, 1126, 1141, 1572, 2036
AfiI CCNNNNNNNGG 2 cut(s) 1007, 1322
AjnI CCWGG 5 cut(s) 654, 1321, 1459, 1747, 2077
AjuI GAANNNNNNNTTGG 2 cut(s) 2222, 2254
AleI CACNNNNGTG 1 cut(s) 1053
Alw21I GWGCWC 3 cut(s) 133, 1492, 2269
Alw26I GTCTC 2 cut(s) 147, 2253
Alw44I GTGCAC 1 cut(s) 2265
AlwI GGATC 3 cut(s) 197, 480, 2033
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 3 cut(s) 6, 690, 1750
ApaLI GTGCAC 1 cut(s) 2265
ApeKI GCWGC 4 cut(s) 90, 791, 1856, 2155
ApoI RAATTY 5 cut(s) 242, 481, 1294, 1455, 2176
ArsI GACNNNNNNTTYG 2 cut(s) 1931, 1963
Asp700I GAANNNNTTC 1 cut(s) 1110
Asp718I GGTACC 1 cut(s) 992
AspLEI GCGC 1 cut(s) 1035
AspS9I GGNCC 3 cut(s) 227, 874, 1751
AsuHPI GGTGA 4 cut(s) 644, 1029, 1597, 1970
AsuII TTCGAA 1 cut(s) 1718
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 2 cut(s) 227, 874
BaeGI GKGCMC 1 cut(s) 2269
BaeI ACNNNNGTAYC 2 cut(s) 2200, 2233
BanI GGYRCC 2 cut(s) 992, 2165
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 751, 783
BauI CACGAG 1 cut(s) 2369
BbsI GAAGAC 2 cut(s) 453, 2081
Bbv12I GWGCWC 3 cut(s) 133, 1492, 2269
BbvI GCAGC 4 cut(s) 77, 803, 1868, 2142
BccI CCATC 7 cut(s) 794, 1150, 1706, 1807, 2134, 2311, 2317
BceAI ACGGC 1 cut(s) 1107
BcgI CGANNNNNNTGC 2 cut(s) 1980, 2014
BciT130I CCWGG 5 cut(s) 656, 1323, 1461, 1749, 2079
BciVI GTATCC 4 cut(s) 226, 611, 1162, 1753
BclI TGATCA 2 cut(s) 400, 1309
BcoDI GTCTC 2 cut(s) 147, 2253
BfaI CTAG 3 cut(s) 464, 518, 1440
BfmI CTRYAG 2 cut(s) 789, 1089
BfuI GTATCC 4 cut(s) 226, 611, 1162, 1753
BglII AGATCT 2 cut(s) 1174, 1589
BisI GCNGC 8 cut(s) 6, 91, 508, 792, 795, 1009, 1857, 2156
BlpI GCTNAGC 1 cut(s) 747
BlsI GCNGC 8 cut(s) 7, 92, 509, 793, 796, 1010, 1858, 2157
Bme1390I CCNGG 5 cut(s) 656, 1323, 1461, 1749, 2079
Bme18I GGWCC 2 cut(s) 227, 874
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 3 cut(s) 227, 874, 1751
BmiI GGNNCC 5 cut(s) 642, 994, 1620, 1753, 2167
BmrFI CCNGG 5 cut(s) 656, 1323, 1461, 1749, 2079
BmrI ACTGGG 1 cut(s) 1513
BmsI GCATC 9 cut(s) 315, 580, 1103, 1150, 1292, 1517, 1545, 1978, 2119
BmuI ACTGGG 1 cut(s) 1513
BpiI GAAGAC 2 cut(s) 453, 2081
Bpu10I CCTNAGC 1 cut(s) 1494
Bpu1102I GCTNAGC 1 cut(s) 747
Bpu14I TTCGAA 1 cut(s) 1718
BpuEI CTTGAG 1 cut(s) 1715
BsaAI YACGTR 1 cut(s) 146
BsaBI GATNNNNATC 4 cut(s) 594, 804, 1173, 1974
BsaHI GRCGYC 1 cut(s) 370
BsaJI CCNNGG 5 cut(s) 1002, 1519, 1755, 2078, 2191
BsaWI WCCGGW 1 cut(s) 1039
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 2 cut(s) 1007, 1322
Bse1I ACTGG 5 cut(s) 858, 1508, 1713, 1888, 2042
Bse3DI GCAATG 1 cut(s) 1220
Bse8I GATNNNNATC 4 cut(s) 594, 804, 1173, 1974
BseBI CCWGG 5 cut(s) 656, 1323, 1461, 1749, 2079
BseDI CCNNGG 5 cut(s) 1002, 1519, 1755, 2078, 2191
BseGI GGATG 4 cut(s) 595, 1165, 1717, 2328
BseJI GATNNNNATC 4 cut(s) 594, 804, 1173, 1974
BseLI CCNNNNNNNGG 2 cut(s) 1007, 1322
BseMI GCAATG 1 cut(s) 1220
BseMII CTCAG 4 cut(s) 590, 738, 1462, 1485
BseNI ACTGG 5 cut(s) 858, 1508, 1713, 1888, 2042
BseRI GAGGAG 4 cut(s) 482, 952, 2304, 2357
BseSI GKGCMC 1 cut(s) 2269
BseXI GCAGC 4 cut(s) 77, 803, 1868, 2142
BseYI CCCAGC 1 cut(s) 613
BsgI GTGCAG 1 cut(s) 1504
Bsh1236I CGCG 1 cut(s) 1033
BshFI GGCC 3 cut(s) 8, 692, 1752
BshNI GGYRCC 2 cut(s) 992, 2165
BsiHKAI GWGCWC 3 cut(s) 133, 1492, 2269
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 1 cut(s) 1040
BslI CCNNNNNNNGG 2 cut(s) 1007, 1322
BsmAI GTCTC 2 cut(s) 147, 2253
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 3 cut(s) 8, 692, 1752
BsoBI CYCGRG 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 1718
Bsp1286I GDGCHC 3 cut(s) 133, 1492, 2269
Bsp1407I TGTACA 1 cut(s) 1570
Bsp1720I GCTNAGC 1 cut(s) 747
Bsp19I CCATGG 2 cut(s) 1002, 2191
BspACI CCGC 6 cut(s) 5, 189, 508, 794, 1008, 2226
BspANI GGCC 3 cut(s) 8, 692, 1752
BspCNI CTCAG 4 cut(s) 591, 739, 1461, 1486
BspFNI CGCG 1 cut(s) 1033
BspHI TCATGA 2 cut(s) 1914, 1966
BspLI GGNNCC 5 cut(s) 642, 994, 1620, 1753, 2167
BspMAI CTGCAG 1 cut(s) 793
BspPI GGATC 3 cut(s) 197, 480, 2033
BspT104I TTCGAA 1 cut(s) 1718
BspT107I GGYRCC 2 cut(s) 992, 2165
BsrDI GCAATG 1 cut(s) 1220
BsrGI TGTACA 1 cut(s) 1570
BsrI ACTGG 5 cut(s) 858, 1508, 1713, 1888, 2042
BssECI CCNNGG 5 cut(s) 1002, 1519, 1755, 2078, 2191
BssNAI GTATAC 1 cut(s) 761
BssNI GRCGYC 1 cut(s) 370
BssSI CACGAG 1 cut(s) 2369
BssT1I CCWWGG 4 cut(s) 1002, 1519, 1755, 2191
Bst1107I GTATAC 1 cut(s) 761
Bst2BI CACGAG 1 cut(s) 2369
Bst2UI CCWGG 5 cut(s) 656, 1323, 1461, 1749, 2079
Bst6I CTCTTC 2 cut(s) 929, 1357
BstACI GRCGYC 1 cut(s) 370
BstAUI TGTACA 1 cut(s) 1570
BstBAI YACGTR 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 1718
BstC8I GCNNGC 6 cut(s) 267, 1227, 1844, 2170, 2224, 2269
BstDEI CTNAG 6 cut(s) 114, 599, 747, 1448, 1494, 2255
BstDSI CCRYGG 2 cut(s) 1002, 2191
BstF5I GGATG 4 cut(s) 595, 1165, 1717, 2328
BstFNI CGCG 1 cut(s) 1033
BstHHI GCGC 1 cut(s) 1035
BstMAI GTCTC 2 cut(s) 147, 2253
BstNI CCWGG 5 cut(s) 656, 1323, 1461, 1749, 2079
BstNSI RCATGY 3 cut(s) 308, 1846, 2052
BstSCI CCNGG 5 cut(s) 654, 1321, 1459, 1747, 2077
BstSFI CTRYAG 2 cut(s) 789, 1089
BstSLI GKGCMC 1 cut(s) 2269
BstUI CGCG 1 cut(s) 1033
BstV1I GCAGC 4 cut(s) 77, 803, 1868, 2142
BstV2I GAAGAC 2 cut(s) 453, 2081
BstX2I RGATCY 3 cut(s) 472, 1174, 1589
BstXI CCANNNNNNTGG 1 cut(s) 1003
BstYI RGATCY 3 cut(s) 472, 1174, 1589
BstZ17I GTATAC 1 cut(s) 761
BsuI GTATCC 4 cut(s) 226, 611, 1162, 1753
BsuRI GGCC 3 cut(s) 8, 692, 1752
BtgI CCRYGG 2 cut(s) 1002, 2191
BtgZI GCGATG 2 cut(s) 1269, 2330
BtsCI GGATG 4 cut(s) 595, 1165, 1717, 2328
BtsI GCAGTG 1 cut(s) 1492
BtsIMutI CAGTG 8 cut(s) 583, 693, 865, 1057, 1362, 1389, 1492, 2212
Cac8I GCNNGC 6 cut(s) 267, 1227, 1844, 2170, 2224, 2269
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 2 cut(s) 1914, 1966
CfoI GCGC 1 cut(s) 1035
Cfr13I GGNCC 3 cut(s) 227, 874, 1751
CseI GACGC 1 cut(s) 1364
CsiI ACCWGGT 1 cut(s) 654
Csp6I GTAC 7 cut(s) 291, 950, 993, 1125, 1140, 1571, 2035
CviQI GTAC 7 cut(s) 291, 950, 993, 1125, 1140, 1571, 2035
DdeI CTNAG 6 cut(s) 114, 599, 747, 1448, 1494, 2255
DraI TTTAAA 1 cut(s) 1743
DraIII CACNNNGTG 1 cut(s) 658
Eam1104I CTCTTC 2 cut(s) 929, 1357
EarI CTCTTC 2 cut(s) 929, 1357
Ecl136II GAGCTC 1 cut(s) 131
Eco130I CCWWGG 4 cut(s) 1002, 1519, 1755, 2191
Eco24I GRGCYC 1 cut(s) 133
Eco32I GATATC 1 cut(s) 896
Eco47I GGWCC 2 cut(s) 227, 874
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 1174
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 131
EcoO109I RGGNCCY 2 cut(s) 227, 1751
EcoRI GAATTC 1 cut(s) 1455
EcoRII CCWGG 5 cut(s) 654, 1321, 1459, 1747, 2077
EcoRV GATATC 1 cut(s) 896
EcoT14I CCWWGG 4 cut(s) 1002, 1519, 1755, 2191
EcoT22I ATGCAT 2 cut(s) 1538, 1660
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 4 cut(s) 1002, 1519, 1755, 2191
FalI AAGNNNNNCTT 6 cut(s) 270, 302, 696, 728, 2188, 2220
FauI CCCGC 1 cut(s) 2219
FbaI TGATCA 2 cut(s) 400, 1309
FblI GTMKAC 3 cut(s) 760, 1902, 1938
Fnu4HI GCNGC 8 cut(s) 6, 91, 508, 792, 795, 1009, 1857, 2156
FokI GGATG 4 cut(s) 602, 1172, 1724, 2335
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 8 cut(s) 6, 91, 508, 792, 795, 1009, 1857, 2156
FspBI CTAG 3 cut(s) 464, 518, 1440
GlaI GCGC 1 cut(s) 1034
GluI GCNGC 8 cut(s) 6, 91, 508, 792, 795, 1009, 1857, 2156
GsaI CCCAGC 1 cut(s) 617
HaeIII GGCC 3 cut(s) 8, 692, 1752
HapII CCGG 1 cut(s) 1040
HgaI GACGC 1 cut(s) 1364
HhaI GCGC 1 cut(s) 1035
Hin1I GRCGYC 1 cut(s) 370
Hin6I GCGC 1 cut(s) 1033
HinP1I GCGC 1 cut(s) 1033
HincII GTYRAC 2 cut(s) 663, 1939
HindII GTYRAC 2 cut(s) 663, 1939
HpaI GTTAAC 1 cut(s) 663
HpaII CCGG 1 cut(s) 1040
HphI GGTGA 4 cut(s) 644, 1029, 1597, 1970
Hpy188I TCNGA 9 cut(s) 42, 169, 600, 1193, 1560, 1924, 2002, 2008, 2365
Hpy99I CGWCG 3 cut(s) 372, 375, 1380
HpyAV CCTTC 5 cut(s) 19, 714, 865, 1714, 2190
HpyCH4IV ACGT 2 cut(s) 145, 370
HpyF3I CTNAG 6 cut(s) 114, 599, 747, 1448, 1494, 2255
HpySE526I ACGT 2 cut(s) 145, 370
Hsp92I GRCGYC 1 cut(s) 370
HspAI GCGC 1 cut(s) 1033
KpnI GGTACC 1 cut(s) 996
Ksp22I TGATCA 2 cut(s) 400, 1309
KspAI GTTAAC 1 cut(s) 663
LmnI GCTCC 4 cut(s) 128, 1274, 1495, 2327
Lsp1109I GCAGC 4 cut(s) 77, 803, 1868, 2142
LweI GCATC 9 cut(s) 315, 580, 1103, 1150, 1292, 1517, 1545, 1978, 2119
MabI ACCWGGT 1 cut(s) 654
MaeI CTAG 3 cut(s) 464, 518, 1440
MaeII ACGT 2 cut(s) 145, 370
MaeIII GTNAC 5 cut(s) 146, 247, 1346, 1960, 2120
MboII GAAGA 9 cut(s) 149, 278, 445, 458, 903, 946, 1169, 1344, 2081
MflI RGATCY 3 cut(s) 472, 1174, 1589
MhlI GDGCHC 3 cut(s) 133, 1492, 2269
MlyI GAGTC 3 cut(s) 2012, 2075, 2194
MmeI TCCRAC 2 cut(s) 202, 1986
Mph1103I ATGCAT 2 cut(s) 1538, 1660
MroXI GAANNNNTTC 1 cut(s) 1110
MseI TTAA 7 cut(s) 428, 609, 662, 987, 1101, 1515, 1742
MslI CAYNNNNRTG 4 cut(s) 396, 1050, 1053, 1986
MspA1I CMGCKG 3 cut(s) 71, 794, 2226
MspI CCGG 1 cut(s) 1040
MspR9I CCNGG 5 cut(s) 656, 1323, 1461, 1749, 2079
Mva1269I GAATGC 1 cut(s) 494
MvaI CCWGG 5 cut(s) 656, 1323, 1461, 1749, 2079
MvnI CGCG 1 cut(s) 1033
NcoI CCATGG 2 cut(s) 1002, 2191
NlaIV GGNNCC 5 cut(s) 642, 994, 1620, 1753, 2167
NmuCI GTSAC 1 cut(s) 2120
NsiI ATGCAT 2 cut(s) 1538, 1660
NspI RCATGY 3 cut(s) 308, 1846, 2052
NspV TTCGAA 1 cut(s) 1718
OliI CACNNNNGTG 1 cut(s) 1053
PaeI GCATGC 1 cut(s) 1846
PaeR7I CTCGAG 1 cut(s) 20
PagI TCATGA 2 cut(s) 1914, 1966
PctI GAATGC 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 1110
PfeI GAWTC 8 cut(s) 26, 170, 362, 569, 724, 1430, 1561, 2058
PflMI CCANNNNNTGG 1 cut(s) 1322
PfoI TCCNGGA 2 cut(s) 1321, 1459
PkrI GCNGC 8 cut(s) 7, 92, 509, 793, 796, 1010, 1858, 2157
PleI GAGTC 3 cut(s) 2011, 2074, 2194
PpsI GAGTC 3 cut(s) 2011, 2074, 2194
Ppu21I YACGTR 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 227
PsiI TTATAA 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 133
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 5 cut(s) 654, 1321, 1459, 1747, 2077
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 5 cut(s) 654, 1321, 1459, 1747, 2077
PspN4I GGNNCC 5 cut(s) 642, 994, 1620, 1753, 2167
PspPI GGNCC 3 cut(s) 227, 874, 1751
PspPPI RGGWCCY 1 cut(s) 227
PspXI VCTCGAGB 1 cut(s) 20
PsrI GAACNNNNNNTAC 2 cut(s) 1057, 1089
PstI CTGCAG 1 cut(s) 793
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 3 cut(s) 472, 1174, 1589
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 7 cut(s) 292, 951, 994, 1126, 1141, 1572, 2036
RsaNI GTAC 7 cut(s) 291, 950, 993, 1125, 1140, 1571, 2035
RseI CAYNNNNRTG 4 cut(s) 396, 1050, 1053, 1986
SacI GAGCTC 1 cut(s) 133
SalI GTCGAC 1 cut(s) 1937
SaqAI TTAA 7 cut(s) 428, 609, 662, 987, 1101, 1515, 1742
SatI GCNGC 8 cut(s) 6, 91, 508, 792, 795, 1009, 1857, 2156
Sau96I GGNCC 3 cut(s) 227, 874, 1751
SchI GAGTC 3 cut(s) 2012, 2075, 2194
ScrFI CCNGG 5 cut(s) 656, 1323, 1461, 1749, 2079
SduI GDGCHC 3 cut(s) 133, 1492, 2269
SexAI ACCWGGT 1 cut(s) 654
SfaNI GCATC 9 cut(s) 315, 580, 1103, 1150, 1292, 1517, 1545, 1978, 2119
SfcI CTRYAG 2 cut(s) 789, 1089
Sfr274I CTCGAG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 1718
SinI GGWCC 2 cut(s) 227, 874
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 4 cut(s) 396, 1050, 1053, 1986
SmlI CTYRAG 2 cut(s) 20, 1694
SmoI CTYRAG 2 cut(s) 20, 1694
SphI GCATGC 1 cut(s) 1846
SsiI CCGC 6 cut(s) 5, 189, 508, 794, 1008, 2226
SspI AATATT 3 cut(s) 538, 778, 1933
SspMI CTAG 3 cut(s) 464, 518, 1440
SstI GAGCTC 1 cut(s) 133
StyD4I CCNGG 5 cut(s) 654, 1321, 1459, 1747, 2077
StyI CCWWGG 4 cut(s) 1002, 1519, 1755, 2191
TaiI ACGT 2 cut(s) 148, 373
TaqI TCGA 9 cut(s) 21, 135, 498, 1180, 1378, 1419, 1539, 1718, 1938
TatI WGTACW 1 cut(s) 1570
TauI GCSGC 4 cut(s) 8, 510, 797, 1011
TfiI GAWTC 8 cut(s) 26, 170, 362, 569, 724, 1430, 1561, 2058
Tru1I TTAA 7 cut(s) 428, 609, 662, 987, 1101, 1515, 1742
Tru9I TTAA 7 cut(s) 428, 609, 662, 987, 1101, 1515, 1742
TscAI CASTG 8 cut(s) 583, 700, 865, 1057, 1369, 1396, 1492, 2219
TseFI GTSAC 1 cut(s) 2120
TseI GCWGC 4 cut(s) 90, 791, 1856, 2155
Tsp45I GTSAC 1 cut(s) 2120
TspGWI ACGGA 3 cut(s) 165, 208, 2301
TspRI CASTG 8 cut(s) 583, 700, 865, 1057, 1369, 1396, 1492, 2219
Van91I CCANNNNNTGG 1 cut(s) 1322
VneI GTGCAC 1 cut(s) 2265
VpaK11BI GGWCC 2 cut(s) 227, 874
XapI RAATTY 5 cut(s) 242, 481, 1294, 1455, 2176
XceI RCATGY 3 cut(s) 308, 1846, 2052
XhoI CTCGAG 1 cut(s) 20
XmiI GTMKAC 3 cut(s) 760, 1902, 1938
XmnI GAANNNNTTC 1 cut(s) 1110
XspI CTAG 3 cut(s) 464, 518, 1440
ZraI GACGTC 1 cut(s) 371
Zsp2I ATGCAT 2 cut(s) 1538, 1660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.