Rorug04G0370500

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007229
Physical Location & Seq
Reverse (-)
54135069 .. 54135522
454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0370500.1

Sequence Viewer

Length: 339 bp
ATGGAAAATGAGGCGGTAGCAGCTGAGACTGCAGTGCCTAAGGTGGCAGTGGTGGTCTGCCTGCTGAGGGGGAAAACAGTTCTGTTGGGACGACGTCGCTCCTCTCTTGGCAACTCAACCTTTTCTCTCCCTGGAGGTCACCTCCAGTTCGGTGAGAGCTTTGAGGAGTGTGCGGCCAGAGAAGTGAAGGAAGAGACTGGTTTGGACATTGACAAGATAGAATTGCTAACAGTCACTAACAATCTGTTTGTAGACGAACCCAAACCATTGCAGTCCGTTGCAATTTTCATTCGAGCAGTGCTACTGCTGGCCTATCCTGATCAAGTTCCTCGGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

112

Amino Acids

12.21

Weight (kDa)

5.15

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 14 - 90 8.7e-18 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 97
AccI GTMKAC 1 cut(s) 252
AciI CCGC 2 cut(s) 14, 173
AcoI YGGCCR 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 94
AfiI CCNNNNNNNGG 1 cut(s) 67
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 2 cut(s) 23, 159
AluI AGCT 2 cut(s) 23, 159
Alw26I GTCTC 2 cut(s) 20, 188
AoxI GGCC 2 cut(s) 174, 309
ApeKI GCWGC 1 cut(s) 20
AsuHPI GGTGA 2 cut(s) 131, 164
AxyI CCTNAGG 1 cut(s) 39
BbvCI CCTCAGC 1 cut(s) 65
BbvI GCAGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 132
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 2 cut(s) 20, 188
BfmI CTRYAG 1 cut(s) 30
BisI GCNGC 2 cut(s) 21, 174
BlsI GCNGC 2 cut(s) 22, 175
Bme1390I CCNGG 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 132
BplI GAGNNNNNCTC 2 cut(s) 126, 158
BpmI CTGGAG 2 cut(s) 128, 153
Bpu10I CCTNAGC 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 94
BsaJI CCNNGG 2 cut(s) 130, 329
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 2 cut(s) 145, 202
Bse21I CCTNAGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 266
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 130, 329
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 266
BseMII CTCAG 2 cut(s) 15, 56
BseNI ACTGG 2 cut(s) 145, 202
BseRI GAGGAG 2 cut(s) 91, 179
BseXI GCAGC 1 cut(s) 32
BshFI GGCC 2 cut(s) 176, 311
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 2 cut(s) 20, 188
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 2 cut(s) 176, 311
Bsp143I GATC 1 cut(s) 319
BspACI CCGC 2 cut(s) 14, 173
BspANI GGCC 2 cut(s) 176, 311
BspCNI CTCAG 2 cut(s) 16, 57
BspMAI CTGCAG 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 266
BsrI ACTGG 2 cut(s) 145, 202
BssECI CCNNGG 2 cut(s) 130, 329
BssMI GATC 1 cut(s) 319
BssNI GRCGYC 1 cut(s) 94
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 79, 232
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 94
BstC8I GCNNGC 2 cut(s) 62, 309
BstDEI CTNAG 3 cut(s) 24, 39, 65
BstEII GGTNACC 1 cut(s) 137
BstKTI GATC 1 cut(s) 322
BstMAI GTCTC 2 cut(s) 20, 188
BstMBI GATC 1 cut(s) 319
BstMWI GCNNNNNNNGC 2 cut(s) 20, 29
BstNI CCWGG 1 cut(s) 132
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 32
Bsu36I CCTNAGG 1 cut(s) 39
BsuRI GGCC 2 cut(s) 176, 311
BtsI GCAGTG 3 cut(s) 39, 54, 303
BtsIMutI CAGTG 3 cut(s) 39, 54, 303
Cac8I GCNNGC 2 cut(s) 62, 309
CviJI RGCY 4 cut(s) 23, 159, 176, 311
CviKI_1 RGCY 4 cut(s) 23, 159, 176, 311
DdeI CTNAG 3 cut(s) 24, 39, 65
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco81I CCTNAGG 1 cut(s) 39
Eco91I GGTNACC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 130
FaqI GGGAC 1 cut(s) 102
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 2 cut(s) 21, 174
Fsp4HI GCNGC 2 cut(s) 21, 174
GluI GCNGC 2 cut(s) 21, 174
GsuI CTGGAG 2 cut(s) 128, 153
HaeIII GGCC 2 cut(s) 176, 311
Hin1I GRCGYC 1 cut(s) 94
HphI GGTGA 2 cut(s) 131, 164
Hpy166II GTNNAC 1 cut(s) 253
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 2 cut(s) 96, 99
HpyAV CCTTC 1 cut(s) 181
HpyCH4III ACNGT 2 cut(s) 79, 232
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 3 cut(s) 32, 271, 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 29
HpyF3I CTNAG 3 cut(s) 24, 39, 65
HpySE526I ACGT 1 cut(s) 94
Hsp92I GRCGYC 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 1 cut(s) 319
LmnI GCTCC 1 cut(s) 104
LpnPI CCDG 8 cut(s) 74, 117, 144, 158, 183, 190, 293, 330
Lsp1109I GCAGC 1 cut(s) 32
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 2 cut(s) 137, 232
MalI GATC 1 cut(s) 321
MboI GATC 1 cut(s) 319
MboII GAAGA 1 cut(s) 203
MluCI AATT 2 cut(s) 221, 282
MnlI CCTC 7 cut(s) 4, 60, 112, 128, 152, 157, 339
MseI TTAA 1 cut(s) 337
MspA1I CMGCKG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 2 cut(s) 20, 29
NdeII GATC 1 cut(s) 319
NmuCI GTSAC 2 cut(s) 137, 232
PflFI GACNNNGTC 1 cut(s) 93
PkrI GCNGC 2 cut(s) 22, 175
Psp6I CCWGG 1 cut(s) 130
PspEI GGTNACC 1 cut(s) 137
PspGI CCWGG 1 cut(s) 130
PstI CTGCAG 1 cut(s) 34
PsyI GACNNNGTC 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 23
SaqAI TTAA 1 cut(s) 337
SatI GCNGC 2 cut(s) 21, 174
Sau3AI GATC 1 cut(s) 319
ScrFI CCNGG 1 cut(s) 132
SetI ASST 7 cut(s) 25, 45, 97, 122, 139, 144, 161
SfcI CTRYAG 1 cut(s) 30
Sse9I AATT 2 cut(s) 221, 282
SsiI CCGC 2 cut(s) 14, 173
StyD4I CCNGG 1 cut(s) 130
TaaI ACNGT 2 cut(s) 79, 232
TaiI ACGT 1 cut(s) 97
TaqI TCGA 1 cut(s) 292
TasI AATT 2 cut(s) 221, 282
TauI GCSGC 1 cut(s) 176
Tru1I TTAA 1 cut(s) 337
Tru9I TTAA 1 cut(s) 337
TscAI CASTG 3 cut(s) 39, 54, 303
TseFI GTSAC 2 cut(s) 137, 232
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 2 cut(s) 137, 232
TspDTI ATGAA 1 cut(s) 277
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 3 cut(s) 39, 54, 303
Tth111I GACNNNGTC 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 252
ZraI GACGTC 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.