Rroxscaffold_5G00386780

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007229
Physical Location & Seq
Reverse (-)
65553857 .. 65564030
10174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386780.1

Sequence Viewer

Length: 2259 bp
ATGGCAGCATTTATCAAGCTCGAGGATTCCCCAATGTTTCAGAAGCAGGTATGTAATCTAGAACAGACAGCTGATGAGTTGAAAGAGCGATGCCAAAAACTATTCAAAGGGTGTAAGAAGTTTATGGCGGCTCTTGGAGAAGCATGTAATGGCGACACCACTTTTGCAGATTCATTGGAAGCATTTGGTGGTGATCTGGATGATCCTATAAGTGTTGCAATAGGAGGTCCAGTACTATCCAAATTCATTTCTGCATTTCGAGAGCTTGCTACATATAAAGAGCTTCTTCGCACCCAAGTAGAGCATGTGTTAGTCAATCGGTTGATGCACTTTATGTCTGTTGATCTGCAAGATGCGAAGTCACGGGAAAAGTTTGTATCCTTAAAGAAGAATACACGGGGAGACATTGTTTCTGAACTAGAGGAGGTAAATTCCCTAATGAATATTGAAGCAAAAAAGAAGTATGAATTTTTGGAATCTATTAGTGCAATCATGGATGGTCATCTAAGATACTTTAAGCTGGTGTTAACATATGCACAACAATCTAAAGAACAGGCTACTGTTGAACAAGATAAACTTCACAAAAGGATTCAGGAATTTAGGACGCAAGCTGAGATGGACAGTTTACGAGCTTCTAGTAACTTTGAGGCTTCTGCAGGGGTTGAAGGACATCGTGCCTTTGGTTTGGCTTCATACAAAAACACAGAACCAATCACCTTTGAAAATGGGAAGCCACAGACAATCAAGGAAGGATATCTTCTGAAACGATCGTCAAATCTGAGGGGAGACTGGAAGAGAAGGTTTTTCGTACTGAACAGTCAAGGGGCTTTATTTTATTACAGAATCAAGGGTACCAAACCCATGGTGGGTTACATTCCTTTTCTAGGAAATTTAGACTTTATTACAAAGCGGGGATCTCAAGCACATCACTTTACACGTTCAACTGAACATAATAGTGGTGTTTTCGGGAGATTCCGCTCAAAACATAGGGCATCGTCACTTAATGAAAACATTCTGGGCTGCCGAACTGTTGATCTGTGCACTTCAACAATTAAGATGGATGCAGAAGATACGGACCTTCGACTTTGCTTCAGAATAATATCTCCATCGAAAACGTACACGTTGCAGGCTGAAAATGAGGCCGATAGGATGGATTGGATGAACAAAATAACAGGAGCCATTACATCTCTGCTGAATTTTCGGCTTCTTGAACAGCCACATCCGGGAAATGCGAATCTTGAGGGTAGTGAATTAGGTGCTGGTGATGTTCGTCGAGTAGCTAGCTGTGAAAGCTTACATAACATACACTTCAATAGAGTTGATTCTGTTTCTACTGCTCTTAGGGGAATCCCTGGAAATGATCTTTGTGCAGAGTGCAGTAGTCCTGAGCCGGATTGGGCTTCTCTCAATCTTGGGATACTGTTATGCATTGAATGTTCTGGTGCTCATCGGAATCTTGGTGTACATATTTCAAAGGTGAGATCTATAACATTAGATGTCAAAGTTTGGGAACCGACAATTGTGGACTTATTTCGCAACTTGGGTAATGCCTACTGTAATTCTGTGTGGGAGGGAAAGCTTCAACTCAAAAACGAAAGATTGGATGGCTCAAATGCCATTAGGGCTTCAATTTCAAAACCATGTCCCCAAGATGCAATTCAGCATAAGGAAATTTACATCCAAGCAAAGTATGTAGAGAAAGTGATGGTCATTAGAGATGTAGCTGTACCTGTCCTTGCAGCAAGTATATGGGAGGCAGTCAAGACTAGTAACTTACAAGAAGTCTATCGGCTTATTGTGATATCAGATATGACTATCGTCGATACCACCTATGATGATGTGGTTGGCGATGATTTATATCACCATGTTGATGCACAAGACTCAGAGTTGAGTTTTCCCTTAATAGAGAGCAAACAGCACGATCCAGCAGCATGTGAGAGAATCAAGACTTCCAATGATCAAGGGAACTGCCTTCAAGGTTGTTCATTATTGCATCTGGCTTGTCACTCTGGCAATGCAATGATGCTCGAATTGTTGCTGCAATTTGGTGCCAACATAAATAGGCGTGATTTCCATGGAAGGACTCCTTTACACCACTGTATCTGCAGTGGGAATAATTCGCTGGCGAAGTTTCTACTTAGAAGAGGTGCACGCCCATCAATTCAAGACGGAGGAGGGCAAAGCGTACTGGAAAGAGCAATGGAGATGGGAGCGATTACAGATGAGGATCTATTCATAAAACTTTCTGAACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_5 ArfGap BAR_3 PH

Protein Analysis

752

Amino Acids

84.29

Weight (kDa)

6.41

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 7 - 124 2.1e-13 BAR domain of APPL family
PH PF00169 248 - 394 8e-12 PH domain
ArfGap PF01412 444 - 569 8.4e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 646 - 723 5.7e-13 Ankyrin repeats (3 copies)
Ank_5 PF13857 656 - 699 3.9e-06 Ankyrin repeats (many copies)
Ank PF00023 660 - 690 1.6e-06 Ankyrin repeat
Ank_4 PF13637 664 - 713 3.2e-07 Ankyrin repeats (many copies)
Ank PF00023 692 - 723 7.8e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 37
Acc65I GGTACC 1 cut(s) 851
AccB1I GGYRCC 2 cut(s) 851, 2048
AccBSI CCGCTC 1 cut(s) 978
AciI CCGC 3 cut(s) 128, 910, 976
AclWI GGATC 4 cut(s) 197, 922, 1916, 2235
AcsI RAATTY 7 cut(s) 242, 430, 467, 596, 889, 1195, 1671
AcuI CTGAAG 1 cut(s) 1075
AfaI GTAC 7 cut(s) 234, 810, 853, 1118, 1464, 1728, 2187
AfiI CCNNNNNNNGG 3 cut(s) 866, 884, 1223
AflIII ACRYGT 2 cut(s) 935, 1119
AhlI ACTAGT 1 cut(s) 1766
AjnI CCWGG 1 cut(s) 1351
AjuI GAANNNNNNNTTGG 2 cut(s) 1641, 1673
Alw21I GWGCWC 3 cut(s) 1043, 1447, 2152
Alw26I GTCTC 2 cut(s) 396, 780
Alw44I GTGCAC 2 cut(s) 1039, 2148
AlwI GGATC 4 cut(s) 197, 922, 1916, 2235
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 1 cut(s) 1140
ApaLI GTGCAC 2 cut(s) 1039, 2148
ApeKI GCWGC 5 cut(s) 5, 1020, 1739, 1928, 2038
ApoI RAATTY 7 cut(s) 242, 430, 467, 596, 889, 1195, 1671
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
Asp700I GAANNNNTTC 1 cut(s) 1011
Asp718I GGTACC 1 cut(s) 851
AspS9I GGNCC 2 cut(s) 227, 1075
AsuC2I CCSGG 1 cut(s) 1224
AsuHPI GGTGA 5 cut(s) 203, 706, 1274, 1489, 1853
AsuNHI GCTAGC 1 cut(s) 1280
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 2 cut(s) 227, 1075
BaeGI GKGCMC 2 cut(s) 1043, 2152
BaeI ACNNNNGTAYC 2 cut(s) 2083, 2116
BanI GGYRCC 2 cut(s) 851, 2048
Bbv12I GWGCWC 3 cut(s) 1043, 1447, 2152
BbvI GCAGC 5 cut(s) 17, 1007, 1751, 1940, 2025
BccI CCATC 9 cut(s) 491, 610, 1051, 1114, 1144, 1598, 1699, 2164, 2200
BciT130I CCWGG 1 cut(s) 1353
BciVI GTATCC 2 cut(s) 388, 1410
BclI TGATCA 1 cut(s) 1957
BcnI CCSGG 1 cut(s) 1224
BcoDI GTCTC 2 cut(s) 396, 780
BcuI ACTAGT 1 cut(s) 1766
BfaI CTAG 6 cut(s) 59, 419, 636, 884, 1281, 1767
BfmI CTRYAG 2 cut(s) 654, 2104
BfuAI ACCTGC 1 cut(s) 37
BfuI GTATCC 2 cut(s) 388, 1410
BglI GCCNNNNNGGC 1 cut(s) 1622
BglII AGATCT 1 cut(s) 1481
BisI GCNGC 6 cut(s) 6, 129, 1021, 1740, 1929, 2039
BlsI GCNGC 6 cut(s) 7, 130, 1022, 1741, 1930, 2040
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 2 cut(s) 1224, 1353
Bme18I GGWCC 2 cut(s) 227, 1075
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 2 cut(s) 227, 1075
BmiI GGNNCC 4 cut(s) 853, 1177, 1512, 2050
BmrFI CCNGG 2 cut(s) 1224, 1353
BmsI GCATC 9 cut(s) 80, 315, 343, 1001, 1051, 1642, 1861, 2002, 2013
BmtI GCTAGC 1 cut(s) 1284
BoxI GACNNNNGTC 1 cut(s) 1817
Bpu10I CCTNAGC 1 cut(s) 1386
BpuEI CTTGAG 2 cut(s) 903, 1259
BpuMI CCSGG 1 cut(s) 1224
BsaBI GATNNNNATC 3 cut(s) 501, 1857, 2226
BsaJI CCNNGG 3 cut(s) 861, 1351, 2074
BsaXI ACNNNNNCTCC 4 cut(s) 216, 246, 393, 423
Bsc4I CCNNNNNNNGG 3 cut(s) 866, 884, 1223
Bse1I ACTGG 3 cut(s) 230, 794, 2193
Bse3DI GCAATG 3 cut(s) 2020, 2025, 2205
Bse8I GATNNNNATC 3 cut(s) 501, 1857, 2226
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 3 cut(s) 861, 1351, 2074
BseGI GGATG 8 cut(s) 205, 502, 1066, 1155, 1164, 1219, 1609, 1677
BseJI GATNNNNATC 3 cut(s) 501, 1857, 2226
BseLI CCNNNNNNNGG 3 cut(s) 866, 884, 1223
BseMI GCAATG 3 cut(s) 2020, 2025, 2205
BseMII CTCAG 4 cut(s) 603, 770, 1377, 1896
BseNI ACTGG 3 cut(s) 230, 794, 2193
BseRI GAGGAG 2 cut(s) 437, 2187
BseSI GKGCMC 2 cut(s) 1043, 2152
BseXI GCAGC 5 cut(s) 17, 1007, 1751, 1940, 2025
BsgI GTGCAG 2 cut(s) 1389, 1396
Bsh1285I CGRYCG 1 cut(s) 770
BshFI GGCC 1 cut(s) 1142
BshNI GGYRCC 2 cut(s) 851, 2048
BsiEI CGRYCG 1 cut(s) 770
BsiHKAI GWGCWC 3 cut(s) 1043, 1447, 2152
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 2 cut(s) 1223, 1391
BslFI GGGAC 1 cut(s) 1629
BslI CCNNNNNNNGG 3 cut(s) 866, 884, 1223
BsmAI GTCTC 2 cut(s) 396, 780
BsmFI GGGAC 1 cut(s) 1629
BsnI GGCC 1 cut(s) 1142
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 3 cut(s) 1043, 1447, 2152
Bsp1407I TGTACA 1 cut(s) 1462
Bsp19I CCATGG 2 cut(s) 861, 2074
BspACI CCGC 3 cut(s) 128, 910, 976
BspANI GGCC 1 cut(s) 1142
BspCNI CTCAG 4 cut(s) 604, 771, 1378, 1895
BspLI GGNNCC 4 cut(s) 853, 1177, 1512, 2050
BspMAI CTGCAG 2 cut(s) 658, 2108
BspMI ACCTGC 1 cut(s) 37
BspOI GCTAGC 1 cut(s) 1284
BspPI GGATC 4 cut(s) 197, 922, 1916, 2235
BspT107I GGYRCC 2 cut(s) 851, 2048
BsrBI CCGCTC 1 cut(s) 978
BsrDI GCAATG 3 cut(s) 2020, 2025, 2205
BsrGI TGTACA 1 cut(s) 1462
BsrI ACTGG 3 cut(s) 230, 794, 2193
BssECI CCNNGG 3 cut(s) 861, 1351, 2074
BssT1I CCWWGG 2 cut(s) 861, 2074
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 7 cut(s) 562, 623, 818, 1030, 1422, 1556, 2099
Bst6I CTCTTC 2 cut(s) 788, 2137
BstAUI TGTACA 1 cut(s) 1462
BstC8I GCNNGC 6 cut(s) 267, 609, 1128, 1282, 2124, 2152
BstDEI CTNAG 7 cut(s) 506, 612, 779, 1340, 1386, 1882, 2138
BstDSI CCRYGG 2 cut(s) 861, 2074
BstENI CCTNNNNNAGG 1 cut(s) 882
BstF5I GGATG 8 cut(s) 205, 502, 1066, 1155, 1164, 1219, 1609, 1677
BstMAI GTCTC 2 cut(s) 396, 780
BstMCI CGRYCG 1 cut(s) 770
BstMWI GCNNNNNNNGC 2 cut(s) 1290, 1622
BstNI CCWGG 1 cut(s) 1353
BstNSI RCATGY 3 cut(s) 147, 308, 1935
BstPAI GACNNNNGTC 1 cut(s) 1817
BstSCI CCNGG 2 cut(s) 1222, 1351
BstSFI CTRYAG 2 cut(s) 654, 2104
BstSLI GKGCMC 2 cut(s) 1043, 2152
BstV1I GCAGC 5 cut(s) 17, 1007, 1751, 1940, 2025
BstX2I RGATCY 3 cut(s) 914, 1481, 2227
BstXI CCANNNNNNTGG 1 cut(s) 862
BstYI RGATCY 3 cut(s) 914, 1481, 2227
BsuI GTATCC 2 cut(s) 388, 1410
BsuRI GGCC 1 cut(s) 1142
BtgI CCRYGG 2 cut(s) 861, 2074
BtgZI GCGATG 2 cut(s) 103, 1863
BtsCI GGATG 8 cut(s) 205, 502, 1066, 1155, 1164, 1219, 1609, 1677
BtsI GCAGTG 1 cut(s) 2113
BtsIMutI CAGTG 2 cut(s) 2095, 2113
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 6 cut(s) 267, 609, 1128, 1282, 2124, 2152
Cfr13I GGNCC 2 cut(s) 227, 1075
CseI GACGC 1 cut(s) 613
Csp6I GTAC 7 cut(s) 233, 809, 852, 1117, 1463, 1727, 2186
CviAII CATG 9 cut(s) 144, 305, 493, 862, 1641, 1865, 1932, 2075, 2252
CviQI GTAC 7 cut(s) 233, 809, 852, 1117, 1463, 1727, 2186
DdeI CTNAG 7 cut(s) 506, 612, 779, 1340, 1386, 1882, 2138
Eam1104I CTCTTC 2 cut(s) 788, 2137
EarI CTCTTC 2 cut(s) 788, 2137
Eco130I CCWWGG 2 cut(s) 861, 2074
Eco32I GATATC 2 cut(s) 755, 1803
Eco47I GGWCC 2 cut(s) 227, 1075
Eco57I CTGAAG 1 cut(s) 1075
Eco88I CYCGRG 1 cut(s) 20
EcoNI CCTNNNNNAGG 1 cut(s) 882
EcoRII CCWGG 1 cut(s) 1351
EcoRV GATATC 2 cut(s) 755, 1803
EcoT14I CCWWGG 2 cut(s) 861, 2074
EcoT22I ATGCAT 1 cut(s) 1430
ErhI CCWWGG 2 cut(s) 861, 2074
FaeI CATG 9 cut(s) 147, 308, 496, 865, 1644, 1868, 1935, 2078, 2255
FalI AAGNNNNNCTT 8 cut(s) 270, 302, 561, 593, 741, 773, 2071, 2103
FaqI GGGAC 1 cut(s) 1629
FatI CATG 9 cut(s) 143, 304, 492, 861, 1640, 1864, 1931, 2074, 2251
FauI CCCGC 1 cut(s) 903
FauNDI CATATG 1 cut(s) 532
FbaI TGATCA 1 cut(s) 1957
Fnu4HI GCNGC 6 cut(s) 6, 129, 1021, 1740, 1929, 2039
FokI GGATG 8 cut(s) 212, 509, 1073, 1162, 1171, 1206, 1616, 1664
Fsp4HI GCNGC 6 cut(s) 6, 129, 1021, 1740, 1929, 2039
FspBI CTAG 6 cut(s) 59, 419, 636, 884, 1281, 1767
GluI GCNGC 6 cut(s) 6, 129, 1021, 1740, 1929, 2039
HaeIII GGCC 1 cut(s) 1142
HapII CCGG 2 cut(s) 1223, 1391
HgaI GACGC 1 cut(s) 613
Hin1II CATG 9 cut(s) 147, 308, 496, 865, 1644, 1868, 1935, 2078, 2255
HincII GTYRAC 1 cut(s) 528
HindII GTYRAC 1 cut(s) 528
HindIII AAGCTT 2 cut(s) 1291, 1577
HpaI GTTAAC 1 cut(s) 528
HpaII CCGG 2 cut(s) 1223, 1391
HphI GGTGA 5 cut(s) 203, 706, 1274, 1489, 1853
Hpy166II GTNNAC 7 cut(s) 528, 626, 1041, 1119, 1463, 1525, 2150
Hpy188I TCNGA 9 cut(s) 42, 415, 762, 780, 1094, 1452, 1807, 1885, 2248
Hpy8I GTNNAC 7 cut(s) 528, 626, 1041, 1119, 1463, 1525, 2150
Hpy99I CGWCG 2 cut(s) 1275, 1823
HpyAV CCTTC 6 cut(s) 659, 743, 792, 1088, 1982, 2073
HpyCH4III ACNGT 7 cut(s) 562, 623, 818, 1030, 1422, 1556, 2099
HpyCH4IV ACGT 3 cut(s) 937, 1115, 1121
HpyF10VI GCNNNNNNNGC 2 cut(s) 1290, 1622
HpyF3I CTNAG 7 cut(s) 506, 612, 779, 1340, 1386, 1882, 2138
HpySE526I ACGT 3 cut(s) 937, 1115, 1121
Hsp92II CATG 9 cut(s) 147, 308, 496, 865, 1644, 1868, 1935, 2078, 2255
KpnI GGTACC 1 cut(s) 855
Ksp22I TGATCA 1 cut(s) 1957
KspAI GTTAAC 1 cut(s) 528
LmnI GCTCC 2 cut(s) 1175, 2210
Lsp1109I GCAGC 5 cut(s) 17, 1007, 1751, 1940, 2025
LweI GCATC 9 cut(s) 80, 315, 343, 1001, 1051, 1642, 1861, 2002, 2013
MaeI CTAG 6 cut(s) 59, 419, 636, 884, 1281, 1767
MaeII ACGT 3 cut(s) 937, 1115, 1121
MaeIII GTNAC 6 cut(s) 360, 638, 869, 996, 1769, 2003
MbiI CCGCTC 1 cut(s) 978
MboII GAAGA 6 cut(s) 278, 400, 749, 805, 1079, 2154
MfeI CAATTG 1 cut(s) 1518
MflI RGATCY 3 cut(s) 914, 1481, 2227
MhlI GDGCHC 3 cut(s) 1043, 1447, 2152
MlyI GAGTC 2 cut(s) 1874, 2077
Mph1103I ATGCAT 1 cut(s) 1430
MroXI GAANNNNTTC 1 cut(s) 1011
MseI TTAA 6 cut(s) 383, 516, 527, 1002, 1053, 1901
MslI CAYNNNNRTG 2 cut(s) 954, 1869
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 1223, 1391
MspR9I CCNGG 2 cut(s) 1224, 1353
MunI CAATTG 1 cut(s) 1518
MvaI CCWGG 1 cut(s) 1353
MwoI GCNNNNNNNGC 2 cut(s) 1290, 1622
NciI CCSGG 1 cut(s) 1224
NcoI CCATGG 2 cut(s) 861, 2074
NdeI CATATG 1 cut(s) 532
NheI GCTAGC 1 cut(s) 1280
NlaIII CATG 9 cut(s) 147, 308, 496, 865, 1644, 1868, 1935, 2078, 2255
NlaIV GGNNCC 4 cut(s) 853, 1177, 1512, 2050
NmuCI GTSAC 3 cut(s) 360, 996, 2003
NsiI ATGCAT 1 cut(s) 1430
NspI RCATGY 3 cut(s) 147, 308, 1935
PaeR7I CTCGAG 1 cut(s) 20
PdmI GAANNNNTTC 1 cut(s) 1011
PfoI TCCNGGA 1 cut(s) 1222
PkrI GCNGC 6 cut(s) 7, 130, 1022, 1741, 1930, 2040
Ple19I CGATCG 1 cut(s) 770
PleI GAGTC 2 cut(s) 1874, 2077
PpsI GAGTC 2 cut(s) 1874, 2077
PshAI GACNNNNGTC 1 cut(s) 1817
Psp6I CCWGG 1 cut(s) 1351
PspGI CCWGG 1 cut(s) 1351
PspN4I GGNNCC 4 cut(s) 853, 1177, 1512, 2050
PspPI GGNCC 2 cut(s) 227, 1075
PspXI VCTCGAGB 1 cut(s) 20
PstI CTGCAG 2 cut(s) 658, 2108
PsuI RGATCY 3 cut(s) 914, 1481, 2227
PvuI CGATCG 1 cut(s) 770
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 7 cut(s) 234, 810, 853, 1118, 1464, 1728, 2187
RsaNI GTAC 7 cut(s) 233, 809, 852, 1117, 1463, 1727, 2186
RseI CAYNNNNRTG 2 cut(s) 954, 1869
SaqAI TTAA 6 cut(s) 383, 516, 527, 1002, 1053, 1901
SatI GCNGC 6 cut(s) 6, 129, 1021, 1740, 1929, 2039
Sau96I GGNCC 2 cut(s) 227, 1075
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 2 cut(s) 1874, 2077
ScrFI CCNGG 2 cut(s) 1224, 1353
SduI GDGCHC 3 cut(s) 1043, 1447, 2152
SfaNI GCATC 9 cut(s) 80, 315, 343, 1001, 1051, 1642, 1861, 2002, 2013
SfcI CTRYAG 2 cut(s) 654, 2104
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 2 cut(s) 227, 1075
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 2 cut(s) 954, 1869
SmlI CTYRAG 3 cut(s) 20, 918, 1238
SmoI CTYRAG 3 cut(s) 20, 918, 1238
SpeI ACTAGT 1 cut(s) 1766
SsiI CCGC 3 cut(s) 128, 910, 976
SspI AATATT 1 cut(s) 445
SspMI CTAG 6 cut(s) 59, 419, 636, 884, 1281, 1767
StyD4I CCNGG 2 cut(s) 1222, 1351
StyI CCWWGG 2 cut(s) 861, 2074
TaaI ACNGT 7 cut(s) 562, 623, 818, 1030, 1422, 1556, 2099
TaiI ACGT 3 cut(s) 940, 1118, 1124
TaqI TCGA 7 cut(s) 21, 259, 1081, 1109, 1273, 1821, 2028
TatI WGTACW 2 cut(s) 232, 1462
TauI GCSGC 1 cut(s) 131
Tru1I TTAA 6 cut(s) 383, 516, 527, 1002, 1053, 1901
Tru9I TTAA 6 cut(s) 383, 516, 527, 1002, 1053, 1901
TscAI CASTG 2 cut(s) 2102, 2113
TseFI GTSAC 3 cut(s) 360, 996, 2003
TseI GCWGC 5 cut(s) 5, 1020, 1739, 1928, 2038
Tsp45I GTSAC 3 cut(s) 360, 996, 2003
TspDTI ATGAA 9 cut(s) 162, 235, 455, 480, 681, 1020, 1175, 1974, 2224
TspGWI ACGGA 2 cut(s) 1088, 2184
TspRI CASTG 2 cut(s) 2102, 2113
VneI GTGCAC 2 cut(s) 1039, 2148
VpaK11BI GGWCC 2 cut(s) 227, 1075
XagI CCTNNNNNAGG 1 cut(s) 882
XapI RAATTY 7 cut(s) 242, 430, 467, 596, 889, 1195, 1671
XbaI TCTAGA 1 cut(s) 58
XceI RCATGY 3 cut(s) 147, 308, 1935
XcmI CCANNNNNNNNNTGG 1 cut(s) 862
XhoI CTCGAG 1 cut(s) 20
XmnI GAANNNNTTC 1 cut(s) 1011
XspI CTAG 6 cut(s) 59, 419, 636, 884, 1281, 1767
ZrmI AGTACT 1 cut(s) 234
Zsp2I ATGCAT 1 cut(s) 1430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.